##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2375770 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.116140451306315 33.0 31.0 34.0 30.0 34.0 2 32.222267727936625 34.0 31.0 34.0 30.0 34.0 3 32.291464241067104 34.0 31.0 34.0 30.0 34.0 4 35.72283849025789 37.0 35.0 37.0 35.0 37.0 5 35.75436847843015 37.0 35.0 37.0 35.0 37.0 6 35.83389301152889 37.0 37.0 37.0 35.0 37.0 7 35.76269630477698 37.0 36.0 37.0 35.0 37.0 8 35.75720671613835 37.0 36.0 37.0 35.0 37.0 9 37.44314053969871 39.0 38.0 39.0 35.0 39.0 10-11 37.3453301455949 39.0 37.5 39.0 34.5 39.0 12-13 37.24289746061277 39.0 37.0 39.0 34.0 39.0 14-15 38.54649902978824 40.0 38.0 41.0 34.0 41.0 16-17 38.446689283895324 40.0 38.0 41.0 34.0 41.0 18-19 38.325612117334586 40.0 38.0 41.0 34.0 41.0 20-21 38.30604119927434 40.0 38.0 41.0 34.0 41.0 22-23 38.360049583924365 40.0 38.0 41.0 34.0 41.0 24-25 38.31008304675957 40.0 38.0 41.0 34.0 41.0 26-27 38.19533688025356 40.0 38.0 41.0 34.0 41.0 28-29 38.05348771135253 40.0 38.0 41.0 33.0 41.0 30-31 37.85547906573448 40.0 38.0 41.0 33.0 41.0 32-33 37.66562146167348 40.0 38.0 41.0 32.5 41.0 34-35 37.359281832837354 40.0 37.0 41.0 31.5 41.0 36-37 37.10482075285066 40.0 37.0 41.0 31.0 41.0 38-39 36.926162044305634 40.0 37.0 41.0 30.5 41.0 40-41 36.74392386468387 40.0 36.5 41.0 30.0 41.0 42-43 36.540362913918436 40.0 36.0 41.0 30.0 41.0 44-45 36.28347946139567 39.5 36.0 41.0 30.0 41.0 46-47 36.171133358868914 40.0 36.0 41.0 29.0 41.0 48-49 36.065905790543695 40.0 36.0 41.0 28.5 41.0 50-51 34.957958893327216 38.5 34.5 40.0 26.5 40.5 52-53 35.11025898971702 38.5 35.0 39.5 27.0 40.5 54-55 35.6388547712952 39.0 35.0 41.0 28.0 41.0 56-57 35.54670780420663 39.0 35.0 41.0 27.0 41.0 58-59 35.33829263775534 39.0 35.0 41.0 27.0 41.0 60-61 35.041808971407164 38.5 35.0 40.5 26.0 41.0 62-63 34.65626996721063 38.0 34.5 40.0 26.0 41.0 64-65 34.279136658851655 37.5 34.0 40.0 25.0 41.0 66-67 33.893014475306956 37.0 34.0 40.0 23.5 41.0 68-69 33.47419089389966 36.0 34.0 39.0 22.5 41.0 70-71 33.05064273898567 36.0 33.0 39.0 22.0 40.5 72-73 32.599160272248575 35.0 33.0 38.5 20.5 40.0 74-75 32.15048047580363 35.0 32.5 37.0 19.5 39.5 76-77 31.70592965649032 35.0 32.0 37.0 18.0 39.0 78-79 31.26662681993627 35.0 31.5 36.5 15.5 39.0 80-81 30.821974138910754 35.0 31.0 36.0 10.0 37.5 82-83 30.422321815663974 35.0 31.0 35.5 7.0 37.0 84-85 30.036913505937022 34.0 31.0 35.0 2.0 37.0 86-87 29.615840969454112 34.0 30.0 35.0 2.0 36.0 88-89 29.19697824284337 34.0 29.5 35.0 2.0 36.0 90-91 28.790782777794146 34.0 29.0 35.0 2.0 35.5 92-93 28.377122995912906 34.0 29.0 35.0 2.0 35.0 94-95 27.88999566456349 34.0 28.5 35.0 2.0 35.0 96-97 27.284997706006894 34.0 27.0 35.0 2.0 35.0 98-99 26.553102783518607 33.5 25.5 35.0 2.0 35.0 100-101 23.546169241972073 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22337208737977932 1101 2 0.3857729840294226 1101 3 0.26194359280204793 1101 4 0.322366338278826 1101 5 0.23468901578328172 1101 6 0.22351264181109087 1101 7 0.2173184227135181 1101 8 0.29928007507790966 1101 9 0.4361090225764883 1101 10-11 0.3663469479784922 1101 12-13 0.4252570590472686 1101 14-15 0.4967560762184817 1101 16-17 0.43028182851122665 1101 18-19 0.6035229994838147 1101 20-21 0.6153527549624513 1101 22-23 0.6839364217517598 1101 24-25 0.6729069325194104 1101 26-27 0.46614150788569475 1101 28-29 0.3976642541849671 1101 30-31 0.5061554427619583 1101 32-33 0.501221751357491 1101 34-35 0.5071512986348097 1101 36-37 0.5612289785808571 1101 38-39 0.31451863844431927 1101 40-41 0.5149584280386961 1101 42-43 0.4953913884223411 1101 44-45 0.4646798761159445 1101 46-47 0.7038754229347646 1101 48-49 0.5966328802226855 1101 50-51 0.5496124725652507 1101 52-53 0.6633000226722814 1101 54-55 0.747462732878013 1101 56-57 0.7054535765172005 1101 58-59 0.7180617519624874 1101 60-61 0.5367855247849249 1101 62-63 0.5830004740769397 1101 64-65 0.4522800722243403 1101 66-67 0.607595471278735 1101 68-69 0.6439050436100118 1101 70-71 0.5168086284739957 1101 72-73 0.6001612308180597 1101 74-75 0.44614266271246805 1101 76-77 0.3413347747096189 1101 78-79 0.258095167724921 1101 80-81 0.273126631296023 1101 82-83 0.17495271980562066 1101 84-85 0.14653956830115433 1101 86-87 0.13634665718731753 1101 88-89 0.1433549530267122 1101 90-91 0.04750037454472178 1101 92-93 0.22722242460750053 1101 94-95 0.12082796889336933 1101 96-97 0.0468686408680874 1101 98-99 0.02370297179487224 1101 100-101 -0.13623203334525869 1102 1 0.3430198798820854 1102 2 0.33284618933025456 1102 3 0.39913325571278335 1102 4 0.20622999074853254 1102 5 0.2498107883956706 1102 6 0.31715353662128365 1102 7 0.33120357141547885 1102 8 0.35253285659587164 1102 9 0.4173990327340533 1102 10-11 0.4379843230759377 1102 12-13 0.46030536651425535 1102 14-15 0.5317642258889563 1102 16-17 0.5094293021133822 1102 18-19 0.551176130610834 1102 20-21 0.4873043057620592 1102 22-23 0.5238390981507877 1102 24-25 0.5912513683023732 1102 26-27 0.6077786467607993 1102 28-29 0.5580237845749707 1102 30-31 0.5745748791354757 1102 32-33 0.6238943708269318 1102 34-35 0.5210943218082207 1102 36-37 0.5477188328316842 1102 38-39 0.5121192101023624 1102 40-41 0.5033681792965794 1102 42-43 0.4289987396613313 1102 44-45 0.3326168283754143 1102 46-47 0.5249582661067578 1102 48-49 0.4427334400696097 1102 50-51 0.3972868637021918 1102 52-53 0.28752875675679945 1102 54-55 0.36320754189225823 1102 56-57 0.4865546088087882 1102 58-59 0.34613205172863815 1102 60-61 0.28314356697423904 1102 62-63 0.2034614619587316 1102 64-65 0.21141124648555376 1102 66-67 0.15934824098558664 1102 68-69 0.30934807859454594 1102 70-71 0.26412337110976836 1102 72-73 0.2229608197912043 1102 74-75 0.15992151136481425 1102 76-77 0.048671840355805074 1102 78-79 0.036661534389224926 1102 80-81 0.16330155393320922 1102 82-83 0.015070949083018093 1102 84-85 -0.13893807325507623 1102 86-87 -0.11565887041525968 1102 88-89 -0.01014290916118199 1102 90-91 0.08060043832474761 1102 92-93 -0.1507333073273962 1102 94-95 -0.19142134015630674 1102 96-97 -0.1460860280663141 1102 98-99 -0.4795722508446758 1102 100-101 -0.8956478555170868 1103 1 0.10307753104439854 1103 2 0.2636620427119283 1103 3 0.29527677362614924 1103 4 0.25817520150549456 1103 5 0.29315602346059677 1103 6 0.29497480884530347 1103 7 0.2552913919768116 1103 8 0.33679780427640793 1103 9 0.3487235188842419 1103 10-11 0.34958747944267543 1103 12-13 0.3869908145261647 1103 14-15 0.4760314438419968 1103 16-17 0.4938370017769742 1103 18-19 0.40884953700566484 1103 20-21 0.34260562137350803 1103 22-23 0.4010900504039725 1103 24-25 0.35412610659039956 1103 26-27 0.3840502860122115 1103 28-29 0.34913383316797564 1103 30-31 0.3327039452737921 1103 32-33 0.4177892870217761 1103 34-35 0.3953717903855889 1103 36-37 0.3475935092031861 1103 38-39 0.26623505362325517 1103 40-41 0.2414630078910065 1103 42-43 0.26024411025465355 1103 44-45 0.27171596590118696 1103 46-47 0.2967910639768334 1103 48-49 0.06655066249440011 1103 50-51 0.12391085383223555 1103 52-53 0.1424722177986908 1103 54-55 0.13261390003118834 1103 56-57 0.26760413973380537 1103 58-59 0.16340228983206373 1103 60-61 0.2955057740460205 1103 62-63 0.0515740399563569 1103 64-65 -0.028766385138652595 1103 66-67 0.035793361160976644 1103 68-69 0.023867617843883693 1103 70-71 0.04245694522958132 1103 72-73 -0.05807586918834318 1103 74-75 -0.08713378467642485 1103 76-77 0.04271879601210671 1103 78-79 0.0624645572914595 1103 80-81 0.04572918613072119 1103 82-83 0.008038149378382542 1103 84-85 0.058811605122986776 1103 86-87 -0.06284992416568258 1103 88-89 0.029044172758965203 1103 90-91 0.04558905256417134 1103 92-93 0.02862108679649822 1103 94-95 -0.15936199660903938 1103 96-97 -0.29763141861051423 1103 98-99 -0.5891814658578269 1103 100-101 -0.88753552977197 1104 1 0.1797501069060914 1104 2 0.29029700193231633 1104 3 0.1982438880093511 1104 4 0.23478817182906653 1104 5 0.27475490460214047 1104 6 0.3225322452084711 1104 7 0.33663160093075817 1104 8 0.2847829477941275 1104 9 0.36103862175662016 1104 10-11 0.3877482303844104 1104 12-13 0.3836441660610461 1104 14-15 0.38902309598513085 1104 16-17 0.47614892627426997 1104 18-19 0.4310239857754965 1104 20-21 0.4172027887457759 1104 22-23 0.5003306300656334 1104 24-25 0.34165769774570975 1104 26-27 0.4319322644054182 1104 28-29 0.40017283605624954 1104 30-31 0.37745322122498237 1104 32-33 0.4408788392802876 1104 34-35 0.38810543947273146 1104 36-37 0.29124591558969115 1104 38-39 0.17350012790345914 1104 40-41 0.3663133887457377 1104 42-43 0.24568455512150678 1104 44-45 0.26192925599922745 1104 46-47 0.3724093173584748 1104 48-49 0.3392607467037365 1104 50-51 0.36529292313050377 1104 52-53 0.3828241766154079 1104 54-55 0.4080603802889442 1104 56-57 0.36031837520033605 1104 58-59 0.33945906652832747 1104 60-61 0.2813415348156312 1104 62-63 0.1728805546127532 1104 64-65 0.2250330276983732 1104 66-67 0.18923888262359156 1104 68-69 0.07946596015840157 1104 70-71 0.04443352474331874 1104 72-73 0.03714342768641643 1104 74-75 0.03361970592337826 1104 76-77 0.12187739384684804 1104 78-79 0.17852818378220192 1104 80-81 0.11613767776283268 1104 82-83 0.02629509679124098 1104 84-85 -0.11817964466075637 1104 86-87 0.050693233281425165 1104 88-89 -0.08826703198165475 1104 90-91 -0.16279118234503542 1104 92-93 -0.18005255351269156 1104 94-95 -0.17917588557985908 1104 96-97 -0.28360595815333056 1104 98-99 -0.18039070550862846 1104 100-101 -0.2963851692993309 1105 1 -0.046269161828988103 1105 2 0.0885478643418125 1105 3 0.12510967551800434 1105 4 0.10197174806958742 1105 5 0.11403900285613133 1105 6 0.09991479217617183 1105 7 0.033295492618215405 1105 8 0.14673030553784372 1105 9 0.17926654662545616 1105 10-11 0.1217788040502441 1105 12-13 0.05947523373323094 1105 14-15 0.04729545054426154 1105 16-17 0.15287403477510253 1105 18-19 0.1545874836904062 1105 20-21 0.168517901574198 1105 22-23 0.2512917936525838 1105 24-25 0.16800865584266944 1105 26-27 0.13321315106060183 1105 28-29 0.15941057886157495 1105 30-31 0.21914634026300917 1105 32-33 0.08511674721188456 1105 34-35 0.11192230971650474 1105 36-37 0.12542321574508009 1105 38-39 0.16321812224970955 1105 40-41 0.2017370705984547 1105 42-43 -0.010251091094801268 1105 44-45 0.04801031198389438 1105 46-47 0.013620267675193531 1105 48-49 0.10389415136304336 1105 50-51 -0.013111412356330732 1105 52-53 0.10809104855332663 1105 54-55 0.0681276788960119 1105 56-57 -0.0031037531786992645 1105 58-59 0.0634917804022308 1105 60-61 0.003235327311521985 1105 62-63 -0.0012589337702877401 1105 64-65 -0.019594899915418296 1105 66-67 0.031008774656740457 1105 68-69 0.015229051562442919 1105 70-71 -0.05057906130998191 1105 72-73 0.03063209123155275 1105 74-75 0.0371680980009188 1105 76-77 -0.021903521759043088 1105 78-79 -0.01794323929318864 1105 80-81 -0.10639742658196383 1105 82-83 -0.03475980705165327 1105 84-85 -0.17403221306056338 1105 86-87 -0.1574714889697475 1105 88-89 -0.0991430633239112 1105 90-91 -0.09377000800986579 1105 92-93 -0.1115759742085487 1105 94-95 -0.09117065815379988 1105 96-97 0.04953876666266055 1105 98-99 -0.14647988055920536 1105 100-101 -0.15627402430516923 1106 1 0.1926333903808981 1106 2 0.26683573943690675 1106 3 0.2659108138818951 1106 4 0.2955133054912267 1106 5 0.2835368081082592 1106 6 0.27383409734090947 1106 7 0.26548209173145665 1106 8 0.2972841008637559 1106 9 0.30920349840008754 1106 10-11 0.3807584370854471 1106 12-13 0.40628294033864876 1106 14-15 0.38963980562320444 1106 16-17 0.43699725577378246 1106 18-19 0.45052578456411396 1106 20-21 0.38410541206787485 1106 22-23 0.32231890611279823 1106 24-25 0.42579244055797005 1106 26-27 0.4235187170790624 1106 28-29 0.40732353735214843 1106 30-31 0.3540870217549923 1106 32-33 0.324553522963555 1106 34-35 0.3913587654264177 1106 36-37 0.4499578627267198 1106 38-39 0.5672609881497621 1106 40-41 0.7051368987606494 1106 42-43 0.6778996091811749 1106 44-45 0.5449606833554199 1106 46-47 0.45196360846797035 1106 48-49 0.5691419252264609 1106 50-51 0.5512822193051434 1106 52-53 0.5691419671962521 1106 54-55 0.40771352024884777 1106 56-57 0.43816612326055804 1106 58-59 0.5691042049154547 1106 60-61 0.6850153503052852 1106 62-63 0.5396202455441781 1106 64-65 0.3866328664147147 1106 66-67 0.4480650306113674 1106 68-69 0.5066925532623614 1106 70-71 0.4654002541453224 1106 72-73 0.4068313246084614 1106 74-75 0.4566283598935499 1106 76-77 0.49166317124952386 1106 78-79 0.46915046154655116 1106 80-81 0.542198574865612 1106 82-83 0.473402488583222 1106 84-85 0.5563578439807237 1106 86-87 0.40534593705752187 1106 88-89 0.4271335955386597 1106 90-91 0.5540086560373858 1106 92-93 0.567698491137083 1106 94-95 0.6801822814297047 1106 96-97 0.7879667954270317 1106 98-99 0.7654852496608555 1106 100-101 0.6439479123413356 1107 1 0.13915104075980622 1107 2 0.10268383625994204 1107 3 0.04143264953869874 1107 4 0.07970371369910367 1107 5 0.11614644290334297 1107 6 0.04112964262265706 1107 7 0.11675848489066709 1107 8 0.17611973276009252 1107 9 0.1787233396369885 1107 10-11 0.08866663211173176 1107 12-13 0.08019924031425063 1107 14-15 0.07581036528208784 1107 16-17 0.07895412318280393 1107 18-19 0.19106266532460126 1107 20-21 0.2724117717219414 1107 22-23 0.2314375274571958 1107 24-25 0.21007978326420584 1107 26-27 0.21148176458111578 1107 28-29 0.1652179184713276 1107 30-31 0.18063662000547254 1107 32-33 0.14301398179080138 1107 34-35 0.2304829716208161 1107 36-37 0.27365120347490546 1107 38-39 0.23597780463506268 1107 40-41 0.2515272200930738 1107 42-43 0.2377014754617548 1107 44-45 0.24896136080997877 1107 46-47 0.29057389384075094 1107 48-49 0.34819707769523944 1107 50-51 0.23319092182947543 1107 52-53 0.12815630243508025 1107 54-55 0.08085750621913945 1107 56-57 0.12348215739257284 1107 58-59 0.15082799080345666 1107 60-61 0.10233872102509878 1107 62-63 0.16141592303628727 1107 64-65 0.208190980276882 1107 66-67 0.06042693600566196 1107 68-69 0.038591216495113656 1107 70-71 0.013527689118184583 1107 72-73 0.15239615135756424 1107 74-75 0.14128442917888862 1107 76-77 0.1344695247772485 1107 78-79 0.04480630593595336 1107 80-81 0.030516930287362243 1107 82-83 -0.03000395371713438 1107 84-85 0.03707183128299718 1107 86-87 0.05608510954322554 1107 88-89 0.0744084219359884 1107 90-91 0.11727951256681379 1107 92-93 0.13359171734247965 1107 94-95 0.27311796000478594 1107 96-97 0.3148819780373948 1107 98-99 0.2442113386572018 1107 100-101 0.14373279878074285 1108 1 0.18131846456277856 1108 2 0.22462242927441167 1108 3 0.24774073017375997 1108 4 0.33056268080276396 1108 5 0.25166124232330134 1108 6 0.3218733733063033 1108 7 0.12484511018962507 1108 8 0.24700392009098948 1108 9 0.32297239866299776 1108 10-11 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-1.7139931790252447 2316 52-53 -1.4667422063907907 2316 54-55 -1.4104830402690354 2316 56-57 -1.379591020530711 2316 58-59 -1.163221695823438 2316 60-61 -1.0147437987607333 2316 62-63 -1.1201240819259013 2316 64-65 -1.2542097849072462 2316 66-67 -1.3658823091301358 2316 68-69 -1.3791985951933654 2316 70-71 -1.3464611639326662 2316 72-73 -1.4864047964926925 2316 74-75 -1.183305596818677 2316 76-77 -1.2044431083119314 2316 78-79 -0.9624376288651852 2316 80-81 -1.1206364912866604 2316 82-83 -1.1191679684886395 2316 84-85 -0.9772717033067053 2316 86-87 -1.1719831241687473 2316 88-89 -1.294877346286576 2316 90-91 -1.4107721805109286 2316 92-93 -1.2954107745400236 2316 94-95 -1.3944621092501315 2316 96-97 -1.6103884433532407 2316 98-99 -1.8295301168466942 2316 100-101 -1.57372749470764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7846.0 3 2226.0 4 2212.0 5 2512.0 6 2776.0 7 3254.0 8 3983.0 9 4836.0 10 5962.0 11 7161.0 12 9263.0 13 14413.0 14 12297.0 15 11721.0 16 13682.0 17 18980.0 18 16403.0 19 12670.0 20 12751.0 21 13190.0 22 14191.0 23 15754.0 24 17645.0 25 20048.0 26 22954.0 27 27022.0 28 31283.0 29 37259.0 30 44961.0 31 55321.0 32 70376.0 33 91539.0 34 128038.0 35 191670.0 36 313579.0 37 518295.0 38 510249.0 39 87415.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6913970561515 18.54682023965567 14.111610006414368 23.650172697778466 2 15.439569562238256 19.591947159449578 40.78834365910867 24.180139619203498 3 19.664052524452273 23.630567343309494 28.967294426019496 27.738085706218733 4 13.050089686703439 15.951378680189354 36.34827605971921 34.650255573388 5 14.409396683141798 36.594684378380954 33.886845478105435 15.10907346037181 6 33.22752245427323 34.952762097801774 17.282555905590748 14.53715954233425 7 29.588082351513844 31.816090830659725 20.2305646922434 18.365262125583026 8 26.121326905611227 35.58606907498429 19.516960390650933 18.775643628753553 9 26.381776822949575 17.164918488563124 20.18825561312608 36.26504907536122 10-11 25.39263371198008 26.32677832789546 26.87963641824455 21.400951541879913 12-13 26.067250824559064 23.48298298393176 27.606926232952496 22.84283995855668 14-15 22.96640660557832 24.99090667265938 25.733600365797127 26.30908635596517 16-17 22.735817126084708 28.2220640713305 26.41066301145742 22.63145579112737 18-19 22.60313056285128 26.875653113364155 28.4705293554249 22.050686968359663 20-21 23.13185527382549 26.179987029047307 28.55507420593989 22.133083491187314 22-23 22.93514205147499 26.066202353361493 28.403469752576427 22.59518584258709 24-25 22.72985837107352 26.194061798955353 28.56608928538321 22.509990544587914 26-27 22.559887376905397 26.462950950088814 28.314334225719385 22.662827447286404 28-29 22.476456364165724 26.625133407967354 28.47211483808391 22.426295389783004 30-31 22.598311526819685 26.380218443610865 28.36706143860847 22.65440859096098 32-33 22.38178264323311 26.420871716412737 28.41330558650356 22.78404005385059 34-35 22.912614347464245 26.494253880882034 28.212440742255712 22.380691029398005 36-37 22.893573914403063 26.277557118891963 28.164449769501793 22.664419197203177 38-39 22.635953321615858 26.48738326860801 28.258330719148084 22.618332690628044 40-41 22.896467367858307 26.31325401081732 28.205524041218833 22.584754580105535 42-43 22.647765658980994 26.341343923583675 28.07911888372534 22.93177153370999 44-45 22.755950871107633 26.56156354051788 28.027996284255707 22.654489304118776 46-47 22.819081442819602 26.75959621089134 27.6408910303358 22.78043131595326 48-49 22.926480550368733 26.670428258738298 27.59912683673802 22.803964354154953 50-51 22.852139746897144 26.94876040191471 27.56067880546013 22.638421045728016 52-53 23.01043062063293 26.848651068406625 27.402106095134776 22.73881221582567 54-55 22.713522213215047 26.778385715035192 27.54227965753181 22.965812414217947 56-57 22.69044922101356 27.120313371056742 27.45259970916486 22.736637698764838 58-59 22.797646311481824 26.73285939828096 27.52120040990349 22.948293880333726 60-61 22.830150475927994 26.74965570356944 27.662685875688066 22.757507944814503 62-63 22.77410316995823 26.785490306149317 27.77343583927462 22.666970684617826 64-65 22.591205943608834 26.63032025855612 27.66289027359456 23.11558352424048 66-67 22.526463148798374 26.91642795205931 27.509677304683912 23.047431594458406 68-69 22.562299059186433 26.699754003579052 27.751185540218636 22.986761397015876 70-71 22.4892663537726 26.67403933628198 27.657224189889835 23.179470120055587 72-73 22.591046996823884 26.712147759739143 27.52897510120682 23.16783014223015 74-75 22.51973482221997 26.93387715754236 27.35018790585008 23.196200114387594 76-77 22.679241036202963 26.728693754545517 27.429280277340705 23.16278493191081 78-79 22.609995116709378 26.750344569131556 27.458093900877873 23.181566413281196 80-81 22.55795100303738 26.857658559303772 27.43099802055334 23.153392417105515 82-83 22.61280819354799 26.82550148315352 27.34654601752708 23.215144305771407 84-85 22.675059807473872 26.730757434423296 27.27889213925676 23.31529061884607 86-87 22.549001358822107 26.674858237457123 27.543053339267253 23.233087064453517 88-89 22.56187712218205 26.58423646716595 27.46250331330647 23.391383097345525 90-91 22.50401792855154 26.686768299030366 27.502308351790273 23.30690542062782 92-93 22.50988150655946 26.45653829877275 27.538764282691492 23.494815911976303 94-95 22.44107966379841 26.408696859133702 27.610653098083628 23.53957037898427 96-97 22.5619331793247 26.195594630884976 27.570733168829882 23.671739020960437 98-99 22.28626066776495 26.270218871412148 27.456828533306364 23.98669192751654 100-101 22.688175796094807 25.653444517424866 27.93409338744462 23.724286299035708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2046.0 1 1164.0 2 325.0 3 393.5 4 352.5 5 329.5 6 587.5 7 1232.5 8 2009.5 9 2383.5 10 2274.5 11 2164.0 12 2336.0 13 2553.5 14 2781.5 15 3037.0 16 3264.0 17 3411.5 18 3510.5 19 3665.0 20 3875.5 21 4314.0 22 5094.5 23 6340.0 24 7758.0 25 9436.0 26 12048.0 27 15015.5 28 17435.0 29 20427.5 30 23968.5 31 28028.5 32 32650.5 33 36612.0 34 40729.5 35 45152.0 36 50067.0 37 55324.5 38 59086.5 39 62203.0 40 65523.0 41 70142.5 42 75112.5 43 78718.0 44 82528.5 45 86377.5 46 87802.0 47 89316.5 48 93752.5 49 94717.0 50 91405.5 51 89270.0 52 87734.0 53 86956.5 54 86325.5 55 80757.5 56 72859.0 57 66060.0 58 59977.5 59 52714.5 60 44224.0 61 36471.0 62 29618.5 63 23575.5 64 17525.5 65 12700.5 66 9546.5 67 7514.5 68 5888.5 69 4352.5 70 3147.5 71 2339.5 72 1765.0 73 1377.5 74 1038.5 75 722.0 76 496.0 77 298.5 78 213.5 79 148.5 80 96.5 81 72.5 82 52.0 83 35.0 84 26.5 85 19.5 86 15.5 87 14.5 88 14.0 89 13.0 90 12.5 91 8.0 92 3.5 93 3.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38757960576991884 2 0.11549939598530161 3 0.1170146941833595 4 0.1290528965345972 5 0.1306102863492678 6 0.07252385542371526 7 0.08191028592835164 8 0.07938478893158848 9 0.07871132306578499 10-11 0.07717497905942074 12-13 0.0810474077877909 14-15 0.07477575691249574 16-17 0.07603850541087731 18-19 0.08601421854809178 20-21 0.08437264550019573 22-23 0.0938222134297512 24-25 0.1063234235637288 26-27 0.10710211847106413 28-29 0.1014828876532661 30-31 0.0928330604393523 32-33 0.09114939577484352 34-35 0.3384797349911818 36-37 0.5269659941829385 38-39 0.5077722170075386 40-41 0.48043371201757745 42-43 0.5087403241896312 44-45 0.5182951211607184 46-47 0.5160011280553253 48-49 0.5223148705472331 50-51 0.5212204885153024 52-53 0.5195789154674063 54-55 0.5144437382406547 56-57 0.48590562217723093 58-59 0.4760982755064673 60-61 0.42411512898975917 62-63 0.4998169014677346 64-65 0.5258084747260888 66-67 0.5373836692945866 68-69 0.5528312925914545 70-71 0.5467490539909167 72-73 0.5330271869751702 74-75 0.5367312492370895 76-77 0.5126337987263077 78-79 0.48765242426665883 80-81 0.49354525059243953 82-83 0.5074775756912495 84-85 0.5023634442728042 86-87 0.5033315514548967 88-89 0.5132862187838049 90-91 0.5308594687196151 92-93 0.5375099441444248 94-95 0.5330482327834766 96-97 0.5345214393649217 98-99 0.5342688896652453 100-101 0.5102556223876891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2375770.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.78156318242201 #Duplication Level Percentage of deduplicated Percentage of total 1 78.28475637766958 46.017003532344994 2 12.58660698988752 14.797208680567758 3 3.9102909390581497 6.895590416876968 4 1.7191202653791153 4.0421030599025825 5 0.8973977116588354 2.6375220143817386 6 0.5529617086793723 1.9502372169717934 7 0.35959772143191215 1.4796401327823454 8 0.2583579841290463 1.2149348934211777 9 0.19020832853811384 1.006266859360742 >10 1.127872218000026 12.201522527880492 >50 0.07907973450297233 3.1545592796014694 >100 0.03238474238317127 3.4794862273433464 >500 0.0010778515912932163 0.4200199848462239 >1k 1.4371354550555095E-4 0.19619799171444524 >5k 1.4371354550555095E-4 0.5077071820037958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6783 0.28550743548407465 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5237 0.22043379620081066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.1360401048923086 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.2091616612719245E-5 0.0 4 0.0 0.0 0.0 4.2091616612719245E-5 0.0 5 0.0 0.0 0.0 4.2091616612719245E-5 0.0 6 1.2627484983815772E-4 0.0 0.0 4.2091616612719245E-5 0.0 7 1.6836646645087698E-4 0.0 0.0 4.2091616612719245E-5 0.0 8 1.6836646645087698E-4 0.0 0.0 4.2091616612719245E-5 0.0 9 1.6836646645087698E-4 0.0 0.0 4.2091616612719245E-5 0.0 10-11 1.6836646645087698E-4 0.0 0.0 6.313742491907887E-5 0.0 12-13 1.6836646645087698E-4 0.0 0.0 2.3150389136995583E-4 0.0 14-15 1.6836646645087698E-4 0.0 0.0 2.735955079826751E-4 0.0 16-17 2.104580830635962E-4 0.0 0.0 3.9987035782083284E-4 0.0 18-19 2.104580830635962E-4 0.0 0.0 6.10328440884429E-4 0.0 20-21 2.104580830635962E-4 4.2091616612719245E-5 0.0 7.997407156416657E-4 0.0 22-23 2.104580830635962E-4 4.2091616612719245E-5 0.0 0.0010312446070116215 0.0 24-25 2.5254969967631544E-4 4.2091616612719245E-5 0.0 0.0013258859233006562 0.0 26-27 2.5254969967631544E-4 4.2091616612719245E-5 0.0 0.00166261885620241 0.0 28-29 2.9464131628903473E-4 4.2091616612719245E-5 0.0 0.0028622299296649084 0.0 30-31 2.9464131628903473E-4 4.2091616612719245E-5 0.0 0.007071391590936832 0.0 32-33 2.9464131628903473E-4 4.2091616612719245E-5 0.0 0.01607899754605875 0.0 34-35 3.9987035782083284E-4 4.2091616612719245E-5 0.0 0.031021521443574084 0.0 36-37 4.209161661271924E-4 4.2091616612719245E-5 0.0 0.05638172045273743 0.0 38-39 4.209161661271924E-4 4.2091616612719245E-5 0.0 0.09117044158314988 0.0 40-41 4.209161661271924E-4 4.2091616612719245E-5 0.0 0.13101015670708865 0.0 42-43 4.209161661271924E-4 4.2091616612719245E-5 0.0 0.17308072751150153 0.0 44-45 4.209161661271924E-4 4.2091616612719245E-5 0.0 0.22531642372788613 0.0 46-47 4.840535910462713E-4 4.2091616612719245E-5 0.0 0.29045320043606915 0.0 48-49 5.471910159653502E-4 4.2091616612719245E-5 0.0 0.3644502624412296 0.0 50-51 5.471910159653502E-4 4.2091616612719245E-5 0.0 0.4444874714303152 0.0 52-53 5.682368242717099E-4 4.2091616612719245E-5 0.0 0.5263977573586669 0.0 54-55 5.892826325780695E-4 4.2091616612719245E-5 0.0 0.6204935662964007 0.0 56-57 6.524200574971482E-4 4.2091616612719245E-5 0.0 0.733341190435101 0.0 58-59 6.734658658035079E-4 4.2091616612719245E-5 0.0 0.8618889875703456 0.0 60-61 6.945116741098676E-4 4.2091616612719245E-5 0.0 1.004768980162221 0.0 62-63 8.418323322543849E-4 4.2091616612719245E-5 0.0 1.1479857056869984 0.0 64-65 8.418323322543849E-4 4.2091616612719245E-5 0.0 1.3011360527323772 0.0 66-67 8.839239488671041E-4 4.2091616612719245E-5 0.0 1.4809093472853012 0.0 68-69 8.839239488671041E-4 4.2091616612719245E-5 0.0 1.6823177327771628 0.0 70-71 9.260155654798234E-4 4.2091616612719245E-5 0.0 1.9129797918148643 0.0 72-73 0.0010312446070116215 4.2091616612719245E-5 0.0 2.1473038214978724 0.0 74-75 0.0010943820319307004 4.2091616612719245E-5 0.0 2.38486890566006 0.0 76-77 0.0011364736485434195 4.2091616612719245E-5 0.0 2.662042201054816 0.0 78-79 0.0011364736485434195 4.2091616612719245E-5 0.0 2.980802013662939 0.0 80-81 0.001220656881768858 4.2091616612719245E-5 0.0 3.3253008498297394 0.0 82-83 0.0012627484983815773 4.2091616612719245E-5 0.0 3.680701414699234 0.0 84-85 0.0013048401149942965 4.2091616612719245E-5 0.0 4.040479507696452 0.0 86-87 0.0013469317316070159 4.2091616612719245E-5 0.0 4.426817410776296 0.0 88-89 0.001389023348219735 4.2091616612719245E-5 0.0 4.852321563114274 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5375 0.0 35.69388 1 GTATCAA 7940 0.0 35.407238 1 ATCAACG 9705 0.0 28.702442 3 TCAACGC 9875 0.0 28.208326 4 TATCAAC 10005 0.0 27.88915 2 CAACGCA 9960 0.0 27.871292 5 AACGCAG 10405 0.0 26.710749 6 GTACATG 8615 0.0 24.088902 1 TACATGG 8625 0.0 23.398476 2 ACGCAGA 11965 0.0 23.109482 7 CGCAGAG 12100 0.0 22.811558 8 ACATGGG 8770 0.0 22.309383 3 GAGTACT 6560 0.0 21.436625 12-13 GCAGAGT 13240 0.0 20.454075 9 ATTCGCG 200 0.0 20.210901 80-81 GTACTTT 6895 0.0 19.914623 14-15 CATGGGG 5085 0.0 18.81904 4 AGTACTT 7030 0.0 18.420685 12-13 AGAGTAC 11700 0.0 18.02799 10-11 CAGAGTA 12680 0.0 17.549473 10-11 >>END_MODULE