FastQCFastQC Report
Fri 27 May 2016
SRR939188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67520.2842025953690804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54410.2290204859898054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34740.14622627611258665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53250.038.9181181
GTATCAA79850.036.394611
ATCAACG100850.028.6556953
TATCAAC103250.028.1734472
TCAACGC102650.028.1069834
CAACGCA103550.027.8626925
AACGCAG107150.026.9708566
ACGCAGA120750.023.8545467
GTACATG82900.023.7918361
CGCAGAG122900.023.282798
TACATGG84200.023.2195992
GTACCGT1754.0867017E-621.6930946
ACATGGG87100.020.7030833
GCAGAGT136750.020.6471069
GAGTACT70650.019.58048412-13
TACCTGG19600.019.1267262
AGAGTAC121200.019.10775410-11
AGTACTT75350.018.92597412-13
GTACTTT74000.018.59787414-15
CAGAGTA129900.018.32121510-11
Produced by FastQC (version 0.11.5)