FastQCFastQC Report
Fri 27 May 2016
SRR939187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4532811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81190.17911622611222927No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78630.17346851655628262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.11752089376768633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA223850.024.8975721
GTACATG170700.023.8894231
TACATGG168300.023.5339182
ACATGGG168950.022.4058113
TCAACGC250850.021.9086674
ATCAACG251750.021.8303413
CAACGCA252150.021.7393465
TATCAAC254650.021.6189462
AACGCAG259600.021.1080076
CATGGGG89150.019.5568334
GAGTACT103400.019.44398712-13
ACGCAGA284400.019.2509257
CGCAGAG286100.019.0707428
AGAGTAC201700.018.6555910-11
AGTACTT110650.018.40539712-13
GTACTTT110950.017.82292414-15
GCAGAGT312150.017.1751969
GGTATCA166700.016.8874911
GTACGTA3151.3510362E-716.5778481
GAGTACA145550.015.4274781
Produced by FastQC (version 0.11.5)