FastQCFastQC Report
Fri 27 May 2016
SRR939187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4532811
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.19151471349676832No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT82660.182359246833808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58490.12903692653410875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG166800.023.1462141
TACATGG163500.022.7214262
GTATCAA240000.021.9381031
ACATGGG168200.021.0428433
GAGTACT106650.020.10956612-13
TCAACGC270950.019.1741684
ATCAACG272350.019.0930253
TATCAAC274450.019.0679442
CAACGCA273700.018.964185
AGTACTT114900.018.74825912-13
AACGCAG281850.018.4326446
GTACTTT115000.018.25746714-15
CATGGGG91500.018.200264
AGAGTAC211700.017.67282310-11
GGTATCA185300.017.6239741
AGGACCG11550.016.8419915
TATACCG2555.5743567E-616.7453375
ACGCAGA310450.016.7192677
CGCAGAG310350.016.6329298
GTAAGGT18750.016.4476434
Produced by FastQC (version 0.11.5)