##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939187_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4532811 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.532540844963535 34.0 31.0 34.0 31.0 34.0 2 32.73170313079456 34.0 31.0 34.0 31.0 34.0 3 32.834374960703194 34.0 31.0 34.0 31.0 34.0 4 36.28068940002131 37.0 37.0 37.0 35.0 37.0 5 36.2366383685532 37.0 37.0 37.0 35.0 37.0 6 36.26980432230684 37.0 37.0 37.0 35.0 37.0 7 36.226123259937374 37.0 37.0 37.0 35.0 37.0 8 36.211122193270356 37.0 37.0 37.0 35.0 37.0 9 37.96635906504816 39.0 38.0 39.0 35.0 39.0 10-11 37.91091543415333 39.0 38.0 39.0 35.0 39.0 12-13 37.85229099558751 39.0 38.0 39.0 35.0 39.0 14-15 39.24569290446921 41.0 39.0 41.0 36.0 41.0 16-17 39.19694908964878 40.5 39.0 41.0 36.0 41.0 18-19 39.211428184409186 40.5 38.5 41.0 36.0 41.0 20-21 39.26032797308337 41.0 39.0 41.0 36.0 41.0 22-23 39.222319108385506 41.0 39.0 41.0 36.0 41.0 24-25 39.172736520450556 41.0 39.0 41.0 36.0 41.0 26-27 39.04782683416538 40.5 39.0 41.0 35.0 41.0 28-29 38.91269247714057 40.0 38.0 41.0 35.0 41.0 30-31 38.749493084975306 40.0 38.0 41.0 35.0 41.0 32-33 38.63764019721978 40.0 38.0 41.0 34.5 41.0 34-35 38.612543629107854 40.0 38.0 41.0 35.0 41.0 36-37 38.522305584768475 40.0 38.0 41.0 34.0 41.0 38-39 38.40024457229741 40.0 38.0 41.0 34.0 41.0 40-41 38.2518270671334 40.0 38.0 41.0 34.0 41.0 42-43 38.10874333829493 40.0 38.0 41.0 33.0 41.0 44-45 37.9262306546644 40.0 38.0 41.0 33.0 41.0 46-47 37.69500768948893 40.0 37.5 41.0 33.0 41.0 48-49 37.435000929886556 40.0 37.0 41.0 32.5 41.0 50-51 37.210645557469746 40.0 37.0 41.0 32.0 41.0 52-53 36.99197120727072 40.0 36.0 41.0 31.5 41.0 54-55 36.758318734224744 39.0 36.0 41.0 31.0 41.0 56-57 36.48975139267885 39.0 35.0 41.0 31.0 41.0 58-59 36.19751805226382 39.0 35.0 40.0 30.5 41.0 60-61 35.84778893273953 38.0 35.0 40.0 30.0 41.0 62-63 35.47076681555882 38.0 35.0 40.0 29.0 41.0 64-65 35.078278688434175 37.0 34.0 40.0 28.0 41.0 66-67 34.73109787723335 37.0 34.0 39.5 28.0 41.0 68-69 34.27428068366407 36.0 34.0 39.0 28.0 40.5 70-71 33.85602554353137 35.5 33.5 39.0 26.5 40.0 72-73 33.40895318600312 35.0 33.0 38.0 26.0 40.0 74-75 32.934054056081315 35.0 33.0 37.0 26.0 39.0 76-77 30.928793192568584 33.5 30.0 35.5 22.5 37.5 78-79 31.917995588168136 34.5 31.5 36.0 24.5 38.5 80-81 31.9701497591671 35.0 32.0 36.0 25.0 37.5 82-83 31.6926537417951 35.0 32.0 35.5 24.5 37.0 84-85 31.334398125136918 34.5 32.0 35.0 23.5 36.5 86-87 30.819153611302127 34.0 31.0 35.0 20.5 36.0 88-89 30.29837015044307 34.0 31.0 35.0 18.5 36.0 90-91 29.876783854433818 34.0 30.0 35.0 14.5 35.0 92-93 29.635348793497016 34.0 30.0 35.0 9.0 35.0 94-95 29.345872131002153 34.0 30.0 35.0 3.5 35.0 96-97 28.841783498142764 34.0 29.5 35.0 2.0 35.0 98-99 28.12645034174158 34.0 29.0 35.0 2.0 35.0 100-101 24.510930413820475 30.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12199297972315293 1101 2 0.14993438145746296 1101 3 0.193451130464382 1101 4 0.1415366375705318 1101 5 0.12826023349024496 1101 6 0.10896911828087497 1101 7 0.1237344010522321 1101 8 0.16002073723323207 1101 9 0.20450086477973173 1101 10-11 0.18636887011820846 1101 12-13 0.1778475997238118 1101 14-15 0.26902229190383053 1101 16-17 0.2806638853098775 1101 18-19 0.24677512201574103 1101 20-21 0.26868387651101955 1101 22-23 0.23057434003870725 1101 24-25 0.17691683857398743 1101 26-27 0.1657648738311579 1101 28-29 0.1991553564881201 1101 30-31 0.21419703446459692 1101 32-33 0.2356129485795222 1101 34-35 0.3979817058395625 1101 36-37 0.4184101630080548 1101 38-39 0.33700677916043986 1101 40-41 0.3717963992132596 1101 42-43 0.23206800057054977 1101 44-45 0.3887172225908486 1101 46-47 0.2603872835471748 1101 48-49 0.25445014236409236 1101 50-51 0.2941880672548578 1101 52-53 0.2938590414881048 1101 54-55 0.3742346782638606 1101 56-57 0.3946459387387975 1101 58-59 0.42010285530848535 1101 60-61 0.32487570673023924 1101 62-63 0.2655018703578662 1101 64-65 0.28684867766889255 1101 66-67 0.3208480051783624 1101 68-69 0.23579067045106683 1101 70-71 0.263231826032694 1101 72-73 0.27198852350679914 1101 74-75 0.2941975432142314 1101 76-77 0.2327316581052834 1101 78-79 0.3063488751204808 1101 80-81 0.21748062091101872 1101 82-83 0.27186327245379616 1101 84-85 0.2002012629899319 1101 86-87 0.2028861934065631 1101 88-89 0.3628309366251514 1101 90-91 0.3789743642503076 1101 92-93 0.33612155270197164 1101 94-95 0.30821891964520987 1101 96-97 0.21400638949684136 1101 98-99 0.14981606368416678 1101 100-101 -0.042617692708553534 1102 1 0.18605877258181636 1102 2 0.13772998792286018 1102 3 0.2035273045464976 1102 4 0.12386234150985587 1102 5 0.13424432659763852 1102 6 0.17695282762492326 1102 7 0.13895313471817872 1102 8 0.14607515644467384 1102 9 0.20977960478072077 1102 10-11 0.25289512865114006 1102 12-13 0.21597746275559615 1102 14-15 0.27820845537198124 1102 16-17 0.3199694893988223 1102 18-19 0.22909549772463578 1102 20-21 0.2760590300245269 1102 22-23 0.2953675507617177 1102 24-25 0.30878609622073583 1102 26-27 0.29791090526379804 1102 28-29 0.19745757960237142 1102 30-31 0.2669308579733354 1102 32-33 0.2514838184245818 1102 34-35 0.3475665124721914 1102 36-37 0.4048325570048732 1102 38-39 0.37685707222946263 1102 40-41 0.4159662527392243 1102 42-43 0.4158561566661305 1102 44-45 0.43855190034894775 1102 46-47 0.4058887697312059 1102 48-49 0.49240597166066635 1102 50-51 0.4900697700788541 1102 52-53 0.4432011030948644 1102 54-55 0.34775595539166204 1102 56-57 0.37810635962977557 1102 58-59 0.45582043555964447 1102 60-61 0.4656287262515022 1102 62-63 0.4254492025978749 1102 64-65 0.3633118898334686 1102 66-67 0.45214080059359674 1102 68-69 0.3872631743303714 1102 70-71 0.43228212943128597 1102 72-73 0.31304672314676907 1102 74-75 0.3815437477653205 1102 76-77 0.4936291805435076 1102 78-79 0.5147048806736088 1102 80-81 0.4519765980970334 1102 82-83 0.5329713417155624 1102 84-85 0.5850436241870831 1102 86-87 0.609077270287699 1102 88-89 0.6205227275872325 1102 90-91 0.7112562492147951 1102 92-93 0.7038427404593293 1102 94-95 0.5608649417616149 1102 96-97 0.628022414068159 1102 98-99 0.581263900798529 1102 100-101 0.16397081132374325 1103 1 0.12647787434889324 1103 2 0.1061568651447331 1103 3 0.11086066547955653 1103 4 0.08481429422846531 1103 5 0.1571435342045291 1103 6 0.1278127378287195 1103 7 0.09413546793216909 1103 8 0.13097376452429188 1103 9 0.14528729781968508 1103 10-11 0.18618296077737284 1103 12-13 0.11894026088689458 1103 14-15 0.25903064212344873 1103 16-17 0.21577325844742035 1103 18-19 0.23686210400413188 1103 20-21 0.22986070634981814 1103 22-23 0.21349154020942507 1103 24-25 0.13903314822550783 1103 26-27 0.12375529062439483 1103 28-29 0.29186958159671406 1103 30-31 0.3018954242756635 1103 32-33 0.3441824335574637 1103 34-35 0.35215787304389323 1103 36-37 0.4339007175831213 1103 38-39 0.41149830013083744 1103 40-41 0.46662480636560844 1103 42-43 0.4421788796955184 1103 44-45 0.4656466808583346 1103 46-47 0.41953743882409356 1103 48-49 0.4876341906889863 1103 50-51 0.49149882231209574 1103 52-53 0.48741780511728905 1103 54-55 0.4840160986086559 1103 56-57 0.5376434496940519 1103 58-59 0.5423798057544502 1103 60-61 0.5078710259931611 1103 62-63 0.45595788334909315 1103 64-65 0.5127325320738763 1103 66-67 0.4196222595864185 1103 68-69 0.3345961340452561 1103 70-71 0.4466959880179786 1103 72-73 0.4440892093398787 1103 74-75 0.42132034463280377 1103 76-77 0.3711333760844653 1103 78-79 0.4498543203864891 1103 80-81 0.43154651138535627 1103 82-83 0.5215335252930622 1103 84-85 0.4623051402346583 1103 86-87 0.6197528680197166 1103 88-89 0.41040905251312765 1103 90-91 0.578556885311702 1103 92-93 0.5525069842282484 1103 94-95 0.3952104245944632 1103 96-97 0.39752886844185653 1103 98-99 0.3091584726744614 1103 100-101 0.05760445645725909 1104 1 0.091865354001186 1104 2 0.11860658626307696 1104 3 0.14671052411653562 1104 4 0.08120333140023916 1104 5 0.10424535438608729 1104 6 0.13142846035446354 1104 7 0.11381626797142275 1104 8 0.0977093369657851 1104 9 0.15842790733923096 1104 10-11 0.14902711436768357 1104 12-13 0.13540908889559233 1104 14-15 0.18783298140493798 1104 16-17 0.19784201337645158 1104 18-19 0.19594984939400462 1104 20-21 0.1757825792102352 1104 22-23 0.22687242292096954 1104 24-25 0.1680216282929905 1104 26-27 0.2408428426973117 1104 28-29 0.2063601511714097 1104 30-31 0.18236422519068896 1104 32-33 0.25757049242832863 1104 34-35 0.2598983677448885 1104 36-37 0.33005848837512275 1104 38-39 0.35148180960958797 1104 40-41 0.3849763144208467 1104 42-43 0.33212588595561954 1104 44-45 0.371601007533215 1104 46-47 0.3669090057091893 1104 48-49 0.27485786278829494 1104 50-51 0.2716458310618819 1104 52-53 0.3024479962447657 1104 54-55 0.33760615538086824 1104 56-57 0.4084644352736362 1104 58-59 0.44675345519027587 1104 60-61 0.4553031874889726 1104 62-63 0.3750012866646273 1104 64-65 0.36876477133581176 1104 66-67 0.3618071291670404 1104 68-69 0.2876944408377895 1104 70-71 0.2995914035505578 1104 72-73 0.12173439292873667 1104 74-75 0.27872319371451226 1104 76-77 0.2943299816146485 1104 78-79 0.4270424631744838 1104 80-81 0.37106238058301244 1104 82-83 0.4250173858910422 1104 84-85 0.3616211533948537 1104 86-87 0.37211093200908607 1104 88-89 0.41883829375143833 1104 90-91 0.47113554663579293 1104 92-93 0.520006030458088 1104 94-95 0.508869896063004 1104 96-97 0.6056456355381421 1104 98-99 0.5296484395982262 1104 100-101 0.2698793685310008 1105 1 0.04091498964993434 1105 2 0.05084615486642008 1105 3 0.039717708267481555 1105 4 0.0730957722507668 1105 5 0.09356699870575369 1105 6 0.07902258698673137 1105 7 0.08538319514102 1105 8 0.05635486917105226 1105 9 0.07977740347610052 1105 10-11 0.1097117811744397 1105 12-13 0.13582945029227744 1105 14-15 0.05260634525638608 1105 16-17 0.04803316414145087 1105 18-19 0.14452759887264932 1105 20-21 0.12953062464823972 1105 22-23 0.10481023471387374 1105 24-25 0.054393703164898 1105 26-27 0.12629589818660492 1105 28-29 0.0702786602217742 1105 30-31 0.10723153792711315 1105 32-33 0.02874037162511911 1105 34-35 0.14134635958659914 1105 36-37 0.14997018878218427 1105 38-39 0.13665326070695727 1105 40-41 0.10280769551826552 1105 42-43 0.18023875382450427 1105 44-45 0.19077380582346848 1105 46-47 0.14170642206219242 1105 48-49 0.22219515554152736 1105 50-51 0.19436367420502876 1105 52-53 0.1381292617142691 1105 54-55 0.165713454712062 1105 56-57 0.22392341216193046 1105 58-59 0.17862836532914628 1105 60-61 0.24848880359798642 1105 62-63 0.2640337778180779 1105 64-65 0.10913644138275203 1105 66-67 0.15279657861386653 1105 68-69 0.18374009442032246 1105 70-71 0.1938883747891964 1105 72-73 0.11002431290997094 1105 74-75 0.12945855844056808 1105 76-77 0.18017838221370397 1105 78-79 0.2150198638997587 1105 80-81 0.0523625217801964 1105 82-83 0.13801799031964634 1105 84-85 0.2552971062827787 1105 86-87 0.23666783914634948 1105 88-89 0.5573999959403544 1105 90-91 0.4882178962146142 1105 92-93 0.4295008787707353 1105 94-95 0.2076667794374245 1105 96-97 0.25389522399827413 1105 98-99 0.2844066690349685 1105 100-101 -0.15596305273659894 1106 1 0.050662044503631876 1106 2 0.05099851829051971 1106 3 0.07089620908202221 1106 4 0.024302310626083568 1106 5 0.04279197171000959 1106 6 0.06049647271988334 1106 7 0.05073800599932099 1106 8 0.01521539929150606 1106 9 0.034028351417084934 1106 10-11 0.10487197214332866 1106 12-13 0.128158220963293 1106 14-15 0.06668070634323442 1106 16-17 0.03486810662425199 1106 18-19 0.0286716956560511 1106 20-21 0.10922088292443277 1106 22-23 0.0918785993077691 1106 24-25 0.14900256052498406 1106 26-27 0.15072979491144167 1106 28-29 0.14024248147626395 1106 30-31 0.14933589207351616 1106 32-33 0.115440289170607 1106 34-35 0.005334087099576834 1106 36-37 0.16891647966615864 1106 38-39 0.25554592168371215 1106 40-41 0.1360455410456325 1106 42-43 0.17082308100965804 1106 44-45 0.1745950300484509 1106 46-47 0.1800988937618655 1106 48-49 0.13795558776931927 1106 50-51 0.14354189551844598 1106 52-53 0.28645799807829064 1106 54-55 0.25207374981276587 1106 56-57 0.27513803958878924 1106 58-59 0.23156759199899568 1106 60-61 0.15104406939395432 1106 62-63 0.28658633941552836 1106 64-65 0.39746160241962514 1106 66-67 0.4287857678739613 1106 68-69 0.29947306423403575 1106 70-71 0.2887308224319298 1106 72-73 0.13234412508253257 1106 74-75 0.18968129493138974 1106 76-77 0.23124804576465863 1106 78-79 0.33331021847097375 1106 80-81 0.36631265454322914 1106 82-83 0.3564205321317182 1106 84-85 0.4600461363113233 1106 86-87 0.4707552368917405 1106 88-89 0.7103822913441071 1106 90-91 0.6384172888126116 1106 92-93 0.5496391023309322 1106 94-95 0.5213903010371261 1106 96-97 0.7281675835409764 1106 98-99 0.6851840252066701 1106 100-101 0.18727205327622443 1107 1 -0.06160390194139609 1107 2 -0.05252121881220262 1107 3 -8.624279758393527E-4 1107 4 0.030975399563260453 1107 5 0.025493901416723475 1107 6 0.028738988459046766 1107 7 0.010268821864293898 1107 8 -0.046006311385902166 1107 9 0.039812542390457395 1107 10-11 -0.03286860843354589 1107 12-13 -0.04836302578242879 1107 14-15 -0.004949277928631091 1107 16-17 -0.03167952098805671 1107 18-19 0.027590602819365984 1107 20-21 0.03402222616692541 1107 22-23 0.008402003825786153 1107 24-25 -0.04450438999561612 1107 26-27 -0.04782093504356766 1107 28-29 -0.004374761995691756 1107 30-31 7.607476003457236E-4 1107 32-33 -0.03369609942578222 1107 34-35 -0.02311322664673554 1107 36-37 -0.0020606535307692297 1107 38-39 -0.08605878347223239 1107 40-41 -0.13669251619255363 1107 42-43 -0.09746524884003094 1107 44-45 -0.18054472452382697 1107 46-47 -0.15649736561385197 1107 48-49 -0.17237253240105588 1107 50-51 -0.16944552745538743 1107 52-53 -0.15999266590461048 1107 54-55 -0.20388150762494206 1107 56-57 -0.10716260721648752 1107 58-59 -0.20490640602072574 1107 60-61 -0.21202326506547564 1107 62-63 -0.28190905256018084 1107 64-65 -0.21578573693494718 1107 66-67 -0.2767759938912775 1107 68-69 -0.23854187990768594 1107 70-71 -0.17494673938959693 1107 72-73 -0.21840178723375203 1107 74-75 -0.18709996390700923 1107 76-77 -0.1658082434317052 1107 78-79 -0.027711539349869696 1107 80-81 -0.09327659877238048 1107 82-83 -0.14749650519269153 1107 84-85 -0.05136118372645271 1107 86-87 -0.08100069683582944 1107 88-89 -0.07151864117190954 1107 90-91 0.03929559465614929 1107 92-93 0.08939531234694087 1107 94-95 -0.00781680591398981 1107 96-97 0.04642870727454351 1107 98-99 0.2062090914699013 1107 100-101 -0.0014708093389259602 1108 1 -0.04672921963917531 1108 2 -0.0011229910130410303 1108 3 0.001034052511108996 1108 4 0.0032614667151378285 1108 5 -0.006253705045487834 1108 6 0.027019619755527913 1108 7 0.009620297465474437 1108 8 0.025739490753210248 1108 9 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74-75 -0.2582011286289472 2316 76-77 -0.22997511965855466 2316 78-79 -0.1935567812283452 2316 80-81 -0.028717212680859916 2316 82-83 -0.08325886429714657 2316 84-85 -0.21870304524475515 2316 86-87 -0.27411767437337886 2316 88-89 -0.4946254532212251 2316 90-91 -0.5378707787442671 2316 92-93 -0.617181172046223 2316 94-95 -0.24051920083190126 2316 96-97 -0.49753318427960735 2316 98-99 -0.40283676517614353 2316 100-101 0.052795798654823756 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 17.0 5 53.0 6 129.0 7 318.0 8 853.0 9 1616.0 10 2742.0 11 4351.0 12 6632.0 13 9408.0 14 11418.0 15 15024.0 16 19230.0 17 26927.0 18 25557.0 19 18313.0 20 17655.0 21 18382.0 22 19821.0 23 22115.0 24 25759.0 25 30976.0 26 37218.0 27 45791.0 28 56397.0 29 69225.0 30 86104.0 31 107072.0 32 134220.0 33 176939.0 34 248963.0 35 372833.0 36 630391.0 37 1117897.0 38 1065527.0 39 106921.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.579875521427795 19.207248233720076 14.499810067906257 23.713066176945873 2 15.499388789870128 20.58062866508222 39.91995254159064 24.000030003457017 3 19.83054223968306 24.196221726429805 29.058039260847185 26.915196773039952 4 13.501423288992195 16.43781309214084 36.65030375191024 33.410459866956735 5 14.332342557410843 36.933483438863874 33.7736561264081 14.960517877317189 6 32.51037821784319 35.515092069799515 17.373876828308084 14.600652884049214 7 28.984861711639866 31.995090904959415 20.729829679640293 18.290217703760426 8 25.811003370756026 35.05509053874075 19.987773591265995 19.14613249923723 9 26.163720481617258 17.492522851713872 20.566994741232318 35.77676192543656 10-11 25.11916998083529 26.382856024661077 27.122463301470102 21.37551069303353 12-13 26.10847882252315 23.621313573409523 27.48948720782755 22.780720396239772 14-15 22.84473729523925 25.162166666905666 25.954476517009116 26.03861952084597 16-17 22.467569528378615 28.189721195953304 26.63563757470516 22.707071700962917 18-19 22.441589482196417 27.23255852689154 28.369590647919352 21.956261342992683 20-21 23.22659162272594 26.277457851209768 28.30980599014607 22.18614453591822 22-23 22.794541841696024 26.25439269362874 28.185600502646153 22.765464962029082 24-25 22.74645767404038 26.42049606737923 28.33062142831851 22.502424830261884 26-27 22.52391369669415 26.613719505872584 28.271363703952428 22.591003093480836 28-29 22.403018789003116 27.060261281575603 28.14109169784489 22.39562823157639 30-31 22.624758930798315 26.568338340865864 27.996271073251172 22.810631655084645 32-33 22.387575833186073 26.589979154215783 28.089070998106912 22.93337401449123 34-35 22.994591642833306 26.577886134913825 27.94893363523789 22.47858858701498 36-37 23.00294583212972 26.326843704947184 28.01020945221734 22.66000101070576 38-39 22.65217636910959 26.66044987724562 27.999880401093247 22.687493352551538 40-41 22.96597377730501 26.516571160772262 27.93770813654618 22.579746925376547 42-43 22.68412908788716 26.568814711572852 27.68163257561299 23.065423624926996 44-45 22.76335368078023 26.620768074294343 27.76211434598146 22.853763898943967 46-47 23.11522290790981 26.792035532454584 27.28376711085912 22.80897444877649 48-49 22.871717950666714 26.788992586560813 27.368780074662762 22.970509388109708 50-51 22.755084099065282 26.952525464893846 27.48593642485267 22.806454011188208 52-53 23.124202342699498 26.9078969865238 27.140446147584115 22.827454523192593 54-55 22.76094238211123 26.799087806661255 27.344786711821868 23.095183099405645 56-57 22.819107172127847 26.937986163552814 27.224618454199835 23.018288210119504 58-59 22.97122915301099 26.660197250634237 27.21457994722245 23.15399364913232 60-61 22.719554455117866 26.835664065654413 27.502185406295826 22.942596072931895 62-63 22.613558180403988 26.778167998364744 27.559338453475547 23.048935367755725 64-65 22.440130115975478 26.95933164273901 27.34717776313611 23.253360478149403 66-67 22.502939249912686 26.85397779959004 27.167913296926127 23.47516965357115 68-69 22.695530371907527 26.633682348484633 27.39693191126886 23.27385536833898 70-71 22.762411973763296 26.56978490497231 27.149690021235166 23.51811310002922 72-73 22.676899610418346 26.50679677577556 27.343584367404684 23.472719246401404 74-75 22.493029160051016 27.02358426151013 27.001589080153575 23.48179749828528 76-77 22.59444929378044 26.63426307927824 27.176408525751743 23.594879101189576 78-79 22.626774251584262 26.67658549924478 27.2109692228288 23.485671026342157 80-81 22.66383962693842 26.709303914823458 27.022585982414505 23.604270475823615 82-83 22.63495342599452 26.68069924324687 27.209601335690675 23.474745995067934 84-85 22.57485476451588 26.7013008042912 27.044774203027654 23.679070228165262 86-87 22.48305257313333 26.68926544438766 27.092052565624208 23.7356294168548 88-89 22.525564117385994 26.702303896750905 26.846944694360893 23.925187291502212 90-91 22.423087115903478 26.50747325861053 27.029637323556443 24.03980230192955 92-93 22.478413505438457 26.509444139629913 27.08945949875254 23.92268285617909 94-95 22.32607977698607 26.331783963637573 27.214801146573286 24.12733511280307 96-97 22.242412048505884 26.11123649320477 27.360108330128917 24.28624312816043 98-99 22.233117018495417 26.332785100419283 26.968481571321572 24.46561630976373 100-101 22.334731077778113 25.733937171876235 27.4775033152951 24.45382843505055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 302.0 1 217.0 2 174.0 3 230.0 4 214.5 5 214.0 6 383.0 7 798.5 8 1305.5 9 1533.5 10 1426.5 11 1379.5 12 1520.0 13 1700.5 14 1928.0 15 2189.5 16 2457.0 17 2792.0 18 3149.5 19 3496.5 20 4027.0 21 4838.0 22 5887.5 23 7398.5 24 9405.5 25 12414.5 26 17281.5 27 23429.0 28 28914.0 29 35829.5 30 44555.5 31 54166.0 32 67051.5 33 76696.0 34 83354.5 35 92993.0 36 104749.5 37 115305.5 38 123208.5 39 129599.5 40 135603.0 41 143273.0 42 148046.0 43 152883.5 44 159645.5 45 165640.5 46 170626.0 47 179392.0 48 195069.5 49 194120.5 50 179002.0 51 169602.0 52 163105.0 53 160217.0 54 157399.0 55 146731.0 56 133897.5 57 121636.5 58 111804.0 59 100957.0 60 83376.5 61 68025.0 62 55912.5 63 41940.0 64 30399.0 65 23464.0 66 17403.0 67 13233.0 68 10265.0 69 7913.5 70 6197.5 71 4495.5 72 3386.0 73 2875.0 74 2219.5 75 1611.0 76 1174.5 77 719.5 78 459.5 79 284.5 80 167.5 81 110.5 82 66.0 83 39.5 84 23.5 85 12.5 86 10.0 87 5.5 88 2.0 89 2.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33989945753308487 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001136160320825201 16-17 0.0016656330916951978 18-19 3.3092048179374784E-5 20-21 0.0 22-23 0.0 24-25 4.301966263318722E-4 26-27 0.0011692523690045758 28-29 0.0 30-31 0.003077560480681855 32-33 0.0 34-35 0.010446056541955974 36-37 0.029209247859661478 38-39 0.021995181356557775 40-41 0.01389866023533741 42-43 0.010765946341023264 44-45 0.003033437749776022 46-47 0.001180283051731034 48-49 6.508102808610374E-4 50-51 4.4122730905833045E-5 52-53 1.3236819271749914E-4 54-55 0.0 56-57 0.0 58-59 0.004456395821489138 60-61 0.010435025859229516 62-63 0.004180628753327681 64-65 0.0018862467462243627 66-67 0.004235782166959972 68-69 0.0033533275488433116 70-71 1.1030682726458261E-5 72-73 0.0 74-75 0.0 76-77 0.21741475653849232 78-79 4.301966263318722E-4 80-81 0.0034084809624756024 82-83 5.51534136322913E-4 84-85 0.0 86-87 0.10997590678278887 88-89 0.40867576433255215 90-91 0.39592429510076643 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014560501198924904 100-101 7.059636944933287E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4532811.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.871134113094655 #Duplication Level Percentage of deduplicated Percentage of total 1 67.63598758299322 31.02539457291087 2 16.27584811791151 14.931832236421567 3 6.543112384508514 9.004199571205218 4 3.1864332289976054 5.846612239590817 5 1.7809512503567444 4.084712682699893 6 1.1010408973340882 3.0303596799368426 7 0.7197165041837621 2.31099486007747 8 0.4797748023188212 1.7606251441000098 9 0.3622558678383452 1.49553787471814 >10 1.7211752630389987 14.01485834669465 >50 0.12374745115664058 3.9240298957445656 >100 0.0641777923139138 5.214498292133318 >500 0.0034191575005652107 1.0825537343268352 >1k 0.0022152281463240823 1.7702221380467256 >5k 1.444714008471798E-4 0.5035687313929572 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8681 0.19151471349676832 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8266 0.182359246833808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5849 0.12903692653410875 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.618409635874957E-5 2 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 3 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 4 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 5 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 6 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 7 0.0 0.0 0.0 2.2061365452916523E-5 6.618409635874957E-5 8 0.0 0.0 0.0 2.2061365452916523E-5 8.824546181166609E-5 9 0.0 0.0 0.0 4.4122730905833045E-5 1.3236819271749914E-4 10-11 0.0 0.0 0.0 6.618409635874957E-5 1.3236819271749914E-4 12-13 0.0 0.0 0.0 7.721477908520783E-5 2.8679775088791477E-4 14-15 0.0 0.0 0.0 8.824546181166609E-5 3.640125299731226E-4 16-17 0.0 0.0 0.0 1.3236819271749914E-4 5.294727708699965E-4 18-19 0.0 0.0 0.0 1.3236819271749914E-4 6.397795981345792E-4 20-21 0.0 0.0 0.0 1.4339887544395738E-4 6.949330117668705E-4 22-23 0.0 0.0 0.0 2.0958297180270698E-4 7.390557426727036E-4 24-25 0.0 0.0 0.0 4.1916594360541396E-4 7.500864253991618E-4 26-27 0.0 0.0 0.0 8.162705217579114E-4 7.831784735785366E-4 28-29 0.0 0.0 0.0 0.0028459161434262314 7.942091563049948E-4 30-31 0.0 0.0 0.0 0.01136160320825201 8.052398390314532E-4 32-33 0.0 0.0 0.0 0.028039995490656902 8.603932526637444E-4 34-35 0.0 0.0 0.0 0.053366443030605074 0.0010037921281077018 36-37 0.0 0.0 0.0 0.09634198293288646 0.0010368841762870766 38-39 0.0 0.0 0.0 0.16479839993328643 0.0010810069071929096 40-41 0.0 0.0 0.0 0.24383324166835987 0.0010810069071929096 42-43 0.0 0.0 0.0 0.3254933858923304 0.0010810069071929096 44-45 0.0 0.0 0.0 0.4232803882623829 0.001147191003551659 46-47 0.0 0.0 0.0 0.54111014114641 0.0012464671480897835 48-49 0.0 0.0 0.0 0.6696506869578281 0.0012795591962691585 50-51 0.0 0.0 0.0 0.812795856699077 0.0012795591962691585 52-53 0.0 0.0 0.0 0.956305038970299 0.0013347126099014497 54-55 0.0 0.0 0.0 1.1178935102301861 0.0013678046580808245 56-57 0.0 0.0 0.0 1.31069881360595 0.0013678046580808245 58-59 0.0 0.0 0.0 1.5233041924757065 0.0013678046580808245 60-61 0.0 0.0 0.0 1.757165696959348 0.0013678046580808245 62-63 0.0 0.0 0.0 1.999245060074201 0.001433988754439574 64-65 0.0 0.0 0.0 2.2588521780413964 0.0014560501198924906 66-67 0.0 0.0 0.0 2.5566033968766844 0.0014560501198924906 68-69 0.0 0.0 0.0 2.889465278830289 0.0014781114853454071 70-71 0.0 0.0 0.0 3.256665676111358 0.0015553262644306149 72-73 0.0 0.0 0.0 3.639540673546724 0.0016104796780629063 74-75 0.0 0.0 0.0 4.038299854108191 0.001687694457148114 76-77 0.0 0.0 0.0 4.4752693196341085 0.0017869706016862385 78-79 0.0 0.0 0.0 4.968484236382237 0.0018310933325920715 80-81 0.0 0.0 0.0 5.507046289818835 0.0018752160634979045 82-83 0.0 0.0 0.0 6.063444957224116 0.001985522890762487 84-85 0.0 0.0 0.0 6.639908436508824 0.001985522890762487 86-87 0.0 0.0 0.0 7.243375027107902 0.00202964562166832 88-89 0.0 0.0 0.0 7.88873615070207 0.002073768352574153 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16680 0.0 23.146214 1 TACATGG 16350 0.0 22.721426 2 GTATCAA 24000 0.0 21.938103 1 ACATGGG 16820 0.0 21.042843 3 GAGTACT 10665 0.0 20.109566 12-13 TCAACGC 27095 0.0 19.174168 4 ATCAACG 27235 0.0 19.093025 3 TATCAAC 27445 0.0 19.067944 2 CAACGCA 27370 0.0 18.96418 5 AGTACTT 11490 0.0 18.748259 12-13 AACGCAG 28185 0.0 18.432644 6 GTACTTT 11500 0.0 18.257467 14-15 CATGGGG 9150 0.0 18.20026 4 AGAGTAC 21170 0.0 17.672823 10-11 GGTATCA 18530 0.0 17.623974 1 AGGACCG 1155 0.0 16.841991 5 TATACCG 255 5.5743567E-6 16.745337 5 ACGCAGA 31045 0.0 16.719267 7 CGCAGAG 31035 0.0 16.632929 8 GTAAGGT 1875 0.0 16.447643 4 >>END_MODULE