FastQCFastQC Report
Fri 27 May 2016
SRR939186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452750
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78560.1764302958845657No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76300.17135478075346694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.11246982202009993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG164250.024.8646181
TACATGG161400.024.5621532
GTATCAA217950.023.3139361
ACATGGG164550.022.7679083
TCAACGC244300.020.4925524
ATCAACG245500.020.3730933
CAACGCA246100.020.3034925
TATCAAC250400.020.0500772
AACGCAG254400.019.7622836
GAGTACT94900.019.20662512-13
CATGGGG87800.018.8267524
AGAGTAC187400.018.49166910-11
AGTACTT102750.018.13090312-13
GTATTAG13150.018.0564751
GTAAGGT17100.018.00374
ACGCAGA278350.017.9088157
CGCAGAG280100.017.7967248
TAAGGTG19000.017.6984255
GTATAGA12500.017.4757791
GAGTACA136650.017.2369631
Produced by FastQC (version 0.11.5)