##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4452750 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22999023075627 33.0 31.0 34.0 31.0 34.0 2 32.33783841446297 34.0 31.0 34.0 31.0 34.0 3 32.415213519735 34.0 31.0 34.0 30.0 34.0 4 35.837844253551175 37.0 35.0 37.0 35.0 37.0 5 35.86956015945202 37.0 35.0 37.0 35.0 37.0 6 35.95264095222054 37.0 37.0 37.0 35.0 37.0 7 35.89009780472742 37.0 37.0 37.0 35.0 37.0 8 35.884660939868624 37.0 37.0 37.0 35.0 37.0 9 37.59040211105496 39.0 38.0 39.0 35.0 39.0 10-11 37.50173128965246 39.0 38.0 39.0 35.0 39.0 12-13 37.414637022065016 39.0 38.0 39.0 35.0 39.0 14-15 38.74874032901016 40.0 38.0 41.0 35.0 41.0 16-17 38.66883667396553 40.0 38.0 41.0 34.5 41.0 18-19 38.55949211161642 40.0 38.0 41.0 34.0 41.0 20-21 38.533324799281345 40.0 38.0 41.0 34.0 41.0 22-23 38.58288181460895 40.0 38.0 41.0 34.0 41.0 24-25 38.54040806243333 40.0 38.0 41.0 34.0 41.0 26-27 38.43671820784908 40.0 38.0 41.0 34.0 41.0 28-29 38.31394239514906 40.0 38.0 41.0 34.0 41.0 30-31 38.135151760148226 40.0 38.0 41.0 33.5 41.0 32-33 37.968344618494186 40.0 38.0 41.0 33.0 41.0 34-35 37.68181460894952 40.0 38.0 41.0 33.0 41.0 36-37 37.440341474369774 40.0 37.5 41.0 32.0 41.0 38-39 37.26571130200438 40.0 37.0 41.0 31.5 41.0 40-41 37.097849418898434 40.0 37.0 41.0 31.0 41.0 42-43 36.90516399977542 40.0 37.0 41.0 31.0 41.0 44-45 36.6684416371905 40.0 36.0 41.0 30.0 41.0 46-47 36.61278973667958 40.0 37.0 41.0 30.0 41.0 48-49 36.53561248666554 40.0 36.0 41.0 30.0 41.0 50-51 35.42355667845713 38.5 34.5 40.0 28.5 40.5 52-53 35.564559991016786 38.5 35.0 40.0 28.5 40.5 54-55 36.104641064510695 39.0 35.5 41.0 29.0 41.0 56-57 36.01435551063949 39.0 35.0 41.0 29.0 41.0 58-59 35.81660760204368 39.0 35.0 41.0 28.5 41.0 60-61 35.522871034753805 39.0 35.0 41.0 28.0 41.0 62-63 35.15572185727921 38.0 35.0 40.0 28.0 41.0 64-65 34.78889641232946 37.5 34.5 40.0 27.0 41.0 66-67 34.40325652686542 37.0 34.0 40.0 26.0 41.0 68-69 33.99183908820392 36.5 34.0 39.0 26.0 41.0 70-71 33.567869294256354 36.0 34.0 39.0 25.5 41.0 72-73 33.13082454662849 35.0 33.0 38.5 25.0 40.0 74-75 32.69454303520297 35.0 33.0 37.5 24.0 39.5 76-77 32.26361563078996 35.0 33.0 37.0 22.5 39.0 78-79 31.83726202908315 35.0 32.0 36.5 21.5 39.0 80-81 31.398616136095672 35.0 32.0 36.0 20.0 37.5 82-83 31.00791084161473 35.0 31.5 35.5 18.5 37.0 84-85 30.631232609061815 35.0 31.0 35.0 16.5 37.0 86-87 30.23524642075122 34.0 31.0 35.0 9.0 36.0 88-89 29.819696928864186 34.0 30.5 35.0 4.5 36.0 90-91 29.414207175341083 34.0 30.0 35.0 2.0 35.5 92-93 29.01291303127281 34.0 29.5 35.0 2.0 35.0 94-95 28.53929021391275 34.0 29.0 35.0 2.0 35.0 96-97 27.953745887372975 34.0 29.0 35.0 2.0 35.0 98-99 27.252264106451072 34.0 27.0 35.0 2.0 35.0 100-101 24.256452529335803 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2194639112334471 1101 2 0.33426853755669583 1101 3 0.20533091316043084 1101 4 0.306468659199858 1101 5 0.25907039556165046 1101 6 0.22264372370968033 1101 7 0.23682098251919115 1101 8 0.3533849024085782 1101 9 0.34534842661911824 1101 10-11 0.3317727928480849 1101 12-13 0.3477128779590757 1101 14-15 0.5327396207792461 1101 16-17 0.5005647925353216 1101 18-19 0.6260905396624707 1101 20-21 0.5602102497032888 1101 22-23 0.5544835172294071 1101 24-25 0.5691116490409343 1101 26-27 0.5241854012240026 1101 28-29 0.4803700107305815 1101 30-31 0.5067986519688787 1101 32-33 0.4890171532678451 1101 34-35 0.5786815343487532 1101 36-37 0.4620024470988682 1101 38-39 0.40967238138516393 1101 40-41 0.4954195884080548 1101 42-43 0.5223674823392841 1101 44-45 0.5192718076570983 1101 46-47 0.6129499842254091 1101 48-49 0.5916479041840148 1101 50-51 0.5678892092725434 1101 52-53 0.5695809972292452 1101 54-55 0.5201030972744363 1101 56-57 0.6136808004475114 1101 58-59 0.4029241943248465 1101 60-61 0.36071884635052953 1101 62-63 0.3433651806556739 1101 64-65 0.26319527849239677 1101 66-67 0.35667669436679006 1101 68-69 0.4037831384875119 1101 70-71 0.24890139194072702 1101 72-73 0.30499491505040766 1101 74-75 0.27137417187272916 1101 76-77 0.12105837968873345 1101 78-79 0.007430234838665228 1101 80-81 -0.08079196801515565 1101 82-83 -0.08974666987482749 1101 84-85 -0.11983533621378228 1101 86-87 -0.13696054492187315 1101 88-89 -0.07350579995165418 1101 90-91 -0.01161866509721321 1101 92-93 -0.20376944498313065 1101 94-95 -0.3334481663967992 1101 96-97 -0.37281249646101955 1101 98-99 -0.4866990825467781 1101 100-101 -0.6320221541235327 1102 1 0.30573665968835684 1102 2 0.31307789279333775 1102 3 0.2873426960231953 1102 4 0.2062894513066169 1102 5 0.2796420619955029 1102 6 0.27634805148473873 1102 7 0.23283489979967698 1102 8 0.26266926817818614 1102 9 0.33890329456150425 1102 10-11 0.36836791033240246 1102 12-13 0.3539065259161873 1102 14-15 0.36377747366856994 1102 16-17 0.40033938436376815 1102 18-19 0.4020238977109045 1102 20-21 0.3821947101079246 1102 22-23 0.4610920612523941 1102 24-25 0.3919013644865714 1102 26-27 0.4189370566344266 1102 28-29 0.5071022646942538 1102 30-31 0.42550724211486823 1102 32-33 0.4096617634414983 1102 34-35 0.38293710346732723 1102 36-37 0.3919996097342491 1102 38-39 0.32362736692570593 1102 40-41 0.3061049012025592 1102 42-43 0.28230419603865897 1102 44-45 0.31867318555668334 1102 46-47 0.36380368807537167 1102 48-49 0.32744520083181783 1102 50-51 0.11816737359460205 1102 52-53 0.2628342669441537 1102 54-55 0.23632015384099958 1102 56-57 0.2618448821939694 1102 58-59 0.1475139836347168 1102 60-61 0.159408318364612 1102 62-63 0.17253735036690188 1102 64-65 0.18988899826194938 1102 66-67 0.11045542039991574 1102 68-69 0.11278321971568062 1102 70-71 0.07783121220724354 1102 72-73 0.15681049112817647 1102 74-75 0.007051312663634235 1102 76-77 -0.10442392311020399 1102 78-79 -0.2328479969393129 1102 80-81 -0.2111880551221148 1102 82-83 -0.38940037507390457 1102 84-85 -0.4089248471274445 1102 86-87 -0.33645327640599376 1102 88-89 -0.31276003681580633 1102 90-91 -0.30805546072741663 1102 92-93 -0.4239313729182861 1102 94-95 -0.6371463809791393 1102 96-97 -0.6679838611982696 1102 98-99 -0.8277554043047743 1102 100-101 -0.960511367781443 1103 1 0.24533335653360666 1103 2 0.3237969953266102 1103 3 0.31967567700095145 1103 4 0.30221757076780875 1103 5 0.3387318730496389 1103 6 0.2942535295777091 1103 7 0.31724726478430654 1103 8 0.32848873152284597 1103 9 0.3707602928551239 1103 10-11 0.3695436976160096 1103 12-13 0.3814744524970948 1103 14-15 0.49612934262971464 1103 16-17 0.5633078077275329 1103 18-19 0.5402938409726659 1103 20-21 0.5050769310816463 1103 22-23 0.544224362978099 1103 24-25 0.48618390450626237 1103 26-27 0.48581159200104906 1103 28-29 0.5700353607882747 1103 30-31 0.5293870237461249 1103 32-33 0.5576562668044645 1103 34-35 0.5970305380428229 1103 36-37 0.5407327116611285 1103 38-39 0.6193034081584585 1103 40-41 0.5975851413179427 1103 42-43 0.5573769966272266 1103 44-45 0.6014611702129855 1103 46-47 0.7168552460795041 1103 48-49 0.8308451754093937 1103 50-51 0.7438019713177013 1103 52-53 0.6845183321994526 1103 54-55 0.6528288703770784 1103 56-57 0.6689589936773643 1103 58-59 0.6180101419316983 1103 60-61 0.6378817951237252 1103 62-63 0.5718758169763021 1103 64-65 0.5560232017173163 1103 66-67 0.5630040738091324 1103 68-69 0.5452931396900595 1103 70-71 0.5451778371048235 1103 72-73 0.6900818183164787 1103 74-75 0.5936845633282175 1103 76-77 0.5730891416514936 1103 78-79 0.5372333135593266 1103 80-81 0.5276258015317126 1103 82-83 0.391462906191137 1103 84-85 0.36663899975910397 1103 86-87 0.3456542593628633 1103 88-89 0.2842880864092301 1103 90-91 0.4131774389756053 1103 92-93 0.2863350783364069 1103 94-95 0.2180982053368794 1103 96-97 0.1385901199924362 1103 98-99 0.05545048214281678 1103 100-101 -0.09598766411890836 1104 1 0.14487825137751287 1104 2 0.2690816550394288 1104 3 0.23498708114542666 1104 4 0.14992372346018357 1104 5 0.19204430782934168 1104 6 0.17079274172310477 1104 7 0.1775779098204353 1104 8 0.1476618348370522 1104 9 0.22517530740580582 1104 10-11 0.23054966125337728 1104 12-13 0.2686929579497601 1104 14-15 0.33457589019899814 1104 16-17 0.31169359306341704 1104 18-19 0.32552378337231147 1104 20-21 0.36690052410541796 1104 22-23 0.40608826862263214 1104 24-25 0.3982719430611752 1104 26-27 0.35545708294563383 1104 28-29 0.38084520648497744 1104 30-31 0.4025499716995711 1104 32-33 0.3786042434002539 1104 34-35 0.33574964627766946 1104 36-37 0.3961225287851917 1104 38-39 0.18928111981681894 1104 40-41 0.3765693237766712 1104 42-43 0.41520635205406364 1104 44-45 0.33236429918259347 1104 46-47 0.529715182542084 1104 48-49 0.5089471888307955 1104 50-51 0.49216178033189095 1104 52-53 0.48838539761690924 1104 54-55 0.5256614915865399 1104 56-57 0.5676535092406709 1104 58-59 0.4997248895641704 1104 60-61 0.5295715160499057 1104 62-63 0.49433452383160414 1104 64-65 0.5146179971409524 1104 66-67 0.4760961136382633 1104 68-69 0.43946830138115445 1104 70-71 0.3569411782148322 1104 72-73 0.4070073187519583 1104 74-75 0.34356178410643423 1104 76-77 0.24836433940693325 1104 78-79 0.3591941036498625 1104 80-81 0.31682183503288286 1104 82-83 0.1664444991723002 1104 84-85 0.2377629051037573 1104 86-87 0.1938223302415274 1104 88-89 0.22660342238237163 1104 90-91 0.22734072915886827 1104 92-93 0.1724616575981237 1104 94-95 0.1035027743811554 1104 96-97 0.08676012429858915 1104 98-99 0.11100022134054655 1104 100-101 0.023967701326505164 1105 1 0.0985283395349299 1105 2 0.2110078475593724 1105 3 0.20446324980481734 1105 4 0.17295097715417995 1105 5 0.20690104544573984 1105 6 0.22612277344773446 1105 7 0.2086916723829546 1105 8 0.18326735002317207 1105 9 0.25967619048457635 1105 10-11 0.24444846591855196 1105 12-13 0.31026398805116884 1105 14-15 0.23247603597031485 1105 16-17 0.27128587473182364 1105 18-19 0.38518758401392716 1105 20-21 0.3081362275203503 1105 22-23 0.31184070614822446 1105 24-25 0.4523078249224639 1105 26-27 0.42373657518098895 1105 28-29 0.30873097323132015 1105 30-31 0.3893590218087368 1105 32-33 0.3958251570325686 1105 34-35 0.45607850064753563 1105 36-37 0.5072572329657135 1105 38-39 0.3815931185790262 1105 40-41 0.4196711031496747 1105 42-43 0.277885911194268 1105 44-45 0.35377993099329075 1105 46-47 0.23704177724340525 1105 48-49 0.35427424394178075 1105 50-51 0.3027981211483848 1105 52-53 0.39048841151972624 1105 54-55 0.3317517885532837 1105 56-57 0.43067718447832704 1105 58-59 0.4014230388728137 1105 60-61 0.33177158962517694 1105 62-63 0.3162448828733986 1105 64-65 0.3239331678649151 1105 66-67 0.34117997164049996 1105 68-69 0.3821353873315587 1105 70-71 0.3670710057141804 1105 72-73 0.4478263589482765 1105 74-75 0.34388997644933994 1105 76-77 0.34069402983661234 1105 78-79 0.2919615042386141 1105 80-81 0.34350685037861695 1105 82-83 0.40340081266513295 1105 84-85 0.35396706820011303 1105 86-87 0.27757074695640327 1105 88-89 0.3367880301050654 1105 90-91 0.3335186318524386 1105 92-93 0.2120131293166594 1105 94-95 0.22799537710755047 1105 96-97 0.2138351337086455 1105 98-99 0.19538477324233128 1105 100-101 0.1816942455733539 1106 1 0.13430608605128214 1106 2 0.23015507243762556 1106 3 0.22147914385811163 1106 4 0.2080740190207635 1106 5 0.23337308357071151 1106 6 0.22807010620170587 1106 7 0.20002329236815086 1106 8 0.20074990412587113 1106 9 0.17282338822727894 1106 10-11 0.21539182617082986 1106 12-13 0.25444318646226094 1106 14-15 0.2636627568795191 1106 16-17 0.27865996042445573 1106 18-19 0.3816142010368182 1106 20-21 0.37312944006240656 1106 22-23 0.35280669840125967 1106 24-25 0.34632190113194383 1106 26-27 0.37764565347442414 1106 28-29 0.3703622934638915 1106 30-31 0.3764635062916071 1106 32-33 0.385388265157502 1106 34-35 0.3541377375530246 1106 36-37 0.3757913253327487 1106 38-39 0.45362062736178643 1106 40-41 0.554972406536784 1106 42-43 0.5665425848376273 1106 44-45 0.43522014316472735 1106 46-47 0.4206536427148677 1106 48-49 0.38207842334013975 1106 50-51 0.42509423617074305 1106 52-53 0.41305203783844036 1106 54-55 0.44240705019591786 1106 56-57 0.40976787458058794 1106 58-59 0.4282039819093413 1106 60-61 0.5681488294684485 1106 62-63 0.4464576160635829 1106 64-65 0.42094365147040236 1106 66-67 0.3176204825410167 1106 68-69 0.41433380009231513 1106 70-71 0.3254213880278982 1106 72-73 0.37923697194748485 1106 74-75 0.29852897723270644 1106 76-77 0.35038165919165465 1106 78-79 0.38560825079329675 1106 80-81 0.33777738133801805 1106 82-83 0.38887448630206833 1106 84-85 0.41481746777108697 1106 86-87 0.4449913177503184 1106 88-89 0.39007028909675157 1106 90-91 0.4285495294845312 1106 92-93 0.39809075936437566 1106 94-95 0.5735028130813404 1106 96-97 0.5563263580956068 1106 98-99 0.5503914964318746 1106 100-101 0.5126232858722659 1107 1 0.07774246327466017 1107 2 0.11564068746784528 1107 3 0.09232170353433844 1107 4 0.12195988042282835 1107 5 0.16791481861444169 1107 6 0.12947250279763978 1107 7 0.16182403225106867 1107 8 0.18801884870145358 1107 9 0.1774824703905722 1107 10-11 0.15717056502050752 1107 12-13 0.19204600597095833 1107 14-15 0.23950354369858218 1107 16-17 0.17676682884098938 1107 18-19 0.22948720126860422 1107 20-21 0.26332452431656606 1107 22-23 0.2446968304593682 1107 24-25 0.2386428905194009 1107 26-27 0.34301772439503253 1107 28-29 0.3733813416912426 1107 30-31 0.401533128457487 1107 32-33 0.39659219203047513 1107 34-35 0.4220812187312859 1107 36-37 0.4127986747341197 1107 38-39 0.48436937344055764 1107 40-41 0.4935760834718721 1107 42-43 0.4823798868807998 1107 44-45 0.46649160562630954 1107 46-47 0.601488289422015 1107 48-49 0.5497555584029641 1107 50-51 0.489985547951882 1107 52-53 0.5261641616310442 1107 54-55 0.5245381847500212 1107 56-57 0.4655039195089188 1107 58-59 0.5654604228201663 1107 60-61 0.5402165677337933 1107 62-63 0.5148065635551902 1107 64-65 0.5346001022216527 1107 66-67 0.489465468244191 1107 68-69 0.5061872110988332 1107 70-71 0.6453901694915984 1107 72-73 0.5561250084004143 1107 74-75 0.5916969770593639 1107 76-77 0.538638607697095 1107 78-79 0.5971803022587352 1107 80-81 0.6813458708388893 1107 82-83 0.6427974546991706 1107 84-85 0.579488870783841 1107 86-87 0.4150180133003012 1107 88-89 0.4384767940719243 1107 90-91 0.5395614777067195 1107 92-93 0.5789711663248092 1107 94-95 0.6149136981760464 1107 96-97 0.6404192025461803 1107 98-99 0.5832754012586747 1107 100-101 0.5145250165464859 1108 1 0.024408425040448378 1108 2 0.0616010246348182 1108 3 0.10004047674192407 1108 4 0.1327279831947763 1108 5 0.14285233555621346 1108 6 0.1704867697671233 1108 7 0.051816397392911995 1108 8 0.16116625031486365 1108 9 0.14576296203071593 1108 10-11 0.18369854895224336 1108 12-13 0.19696917593628172 1108 14-15 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2316 70-71 -1.6058855809531352 2316 72-73 -1.7923883402118648 2316 74-75 -1.5144365781352676 2316 76-77 -1.6475265030944541 2316 78-79 -1.3630495086986762 2316 80-81 -1.4540794597865698 2316 82-83 -1.3422425765878145 2316 84-85 -1.330567924813728 2316 86-87 -1.510808226579158 2316 88-89 -1.6701044144230188 2316 90-91 -1.9405765316621064 2316 92-93 -1.9113700885250111 2316 94-95 -1.7846853094279318 2316 96-97 -2.0461831233839973 2316 98-99 -2.3740426400175068 2316 100-101 -2.2008667151068693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11162.0 3 2699.0 4 2816.0 5 3098.0 6 3724.0 7 4520.0 8 5423.0 9 6999.0 10 8517.0 11 10775.0 12 14534.0 13 23872.0 14 19082.0 15 18296.0 16 21664.0 17 28710.0 18 24225.0 19 19264.0 20 19566.0 21 21223.0 22 23335.0 23 26594.0 24 30312.0 25 34855.0 26 39789.0 27 47532.0 28 56397.0 29 67934.0 30 82380.0 31 101742.0 32 129378.0 33 171628.0 34 239935.0 35 360843.0 36 600341.0 37 997469.0 38 997731.0 39 174361.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24555452519788 18.692717355304076 14.160766910773507 23.900961208724535 2 15.23711494579276 20.16692849014975 40.47040090582606 24.12555565823143 3 19.838119398803713 24.051381430744993 28.862426671109514 27.24807249934178 4 13.290795818805929 16.028720130513342 36.91274337735872 33.767740673322 5 14.409416575387224 36.74434732706584 33.81909512708325 15.02714097046369 6 32.44971256779586 35.24789243727092 17.639992709409512 14.662402285523706 7 29.03162220575966 31.851979882908022 20.8465118746224 18.269886036709917 8 25.75913643924582 34.75713814693056 20.27921701823141 19.204508395592203 9 26.258114164912467 17.508278796885275 20.67961876404429 35.55398827415797 10-11 25.12979295607447 26.149943115849034 27.285614625272565 21.434649302803933 12-13 26.145895059997613 23.465424321147633 27.5315428990507 22.85713771980405 14-15 22.927099510280954 25.007933493501504 25.964829677109062 26.10013731910848 16-17 22.580331962964994 28.04966139346053 26.63048510679613 22.739521536778344 18-19 22.52861187923306 27.16450334430401 28.272711794213624 22.034172982249313 20-21 23.285831373455583 26.247871874511482 28.200965398300692 22.265331353732247 22-23 22.86489069739265 26.212327302914414 28.05643017273024 22.866351826962696 24-25 22.808450272002894 26.33998591508923 28.227993451022336 22.623570361885534 26-27 22.535940829643543 26.55348096395873 28.178986348942736 22.731591857454987 28-29 22.423299794884617 26.9708859348037 28.057262352559263 22.54855191775242 30-31 22.662507507885525 26.573936038210196 27.83586773173741 22.927688722166874 32-33 22.424377089709445 26.540165523476393 27.97919273447314 23.056264652341017 34-35 23.022064302169927 26.585383358926613 27.81570856088705 22.576843778016414 36-37 23.04760356589305 26.249219664565825 27.900956948061552 22.80221982147957 38-39 22.661358788754633 26.646691583038358 27.88852598600593 22.803423642201086 40-41 22.967159081664327 26.4877414538611 27.856789119923235 22.688310344551336 42-43 22.727375333238527 26.534635361996926 27.57019735847097 23.167791946293576 44-45 22.77438701934066 26.601082195694403 27.669901981113217 22.95462880385172 46-47 23.078448617988297 26.793777549821634 27.221074916551945 22.906698915638117 48-49 22.87759426592953 26.741709456664854 27.32512015214757 23.05557612525805 50-51 22.745389716505677 26.929550684813368 27.445273008183808 22.87978659049715 52-53 23.07531759062996 26.84742804134892 27.17045379378321 22.906800574237902 54-55 22.755218541872775 26.750380805269813 27.36332632203907 23.131074330818336 56-57 22.83896746354685 26.885238872226097 27.229447780764293 23.046345883462763 58-59 22.957456108580335 26.587906839458984 27.245321900498865 23.209315151461812 60-61 22.69531963198687 26.730831277394586 27.621137241904364 22.952711848714184 62-63 22.605786699668947 26.707774307562676 27.68060949787615 23.005829494892225 64-65 22.47024688087114 26.871824442175935 27.439630464945296 23.21829821200763 66-67 22.514998588766584 26.779565340107254 27.250364098221848 23.455071972904317 68-69 22.750530251431318 26.556281175983855 27.48558745896649 23.207601113618338 70-71 22.75879387169048 26.465208692742998 27.30091908383604 23.47507835173048 72-73 22.67768705510781 26.44661790492499 27.47986963752929 23.395825402437907 74-75 22.50044087320216 26.89673135140225 27.209926319418905 23.392901455976695 76-77 22.599918868016957 26.53855608998923 27.317809147506928 23.543715894486887 78-79 22.68657423785158 26.545564720199998 27.388527700716153 23.379333341232268 80-81 22.727828768264153 26.573293363492777 27.221806433964673 23.477071434278397 82-83 22.696431788087843 26.518466332976832 27.426083427274833 23.359018451660496 84-85 22.664042784167005 26.55986951763531 27.298572871707876 23.477514826489806 86-87 22.603748742878096 26.513723747722622 27.3693510209393 23.513176488459987 88-89 22.69248911263165 26.473786169749147 27.146651099335532 23.68707361828367 90-91 22.605301698936536 26.320528004607834 27.330377571432134 23.74379272502349 92-93 22.74232542722069 26.23397294410099 27.435853954776174 23.58784767390215 94-95 22.61113681965237 26.058161112135313 27.6003424309959 23.73035963721642 96-97 22.58209641358376 25.801241307617868 27.73587351017571 23.88078876862266 98-99 22.596523472824178 25.994339381938182 27.354118285918787 24.055018859318853 100-101 22.87495100075837 25.326893932852666 27.81452603328438 23.98362903310458 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3314.0 1 1809.5 2 328.0 3 373.0 4 357.0 5 351.0 6 571.5 7 1116.0 8 1718.5 9 2009.5 10 2049.5 11 2136.0 12 2432.5 13 2826.5 14 3228.5 15 3658.0 16 3982.5 17 4182.5 18 4400.5 19 4681.5 20 4998.0 21 5427.0 22 6133.5 23 7416.0 24 9400.0 25 12189.5 26 16675.5 27 22942.5 28 28464.5 29 34871.5 30 43013.0 31 52654.0 32 65550.0 33 74535.0 34 81655.0 35 91867.5 36 103149.5 37 113400.0 38 120685.5 39 126483.5 40 132630.0 41 140133.5 42 144801.0 43 149332.5 44 155495.0 45 161687.5 46 166350.5 47 174210.5 48 189917.5 49 189387.0 50 173703.0 51 164139.0 52 158924.5 53 156073.0 54 152705.0 55 143171.5 56 131162.0 57 118980.0 58 109442.0 59 98578.5 60 81735.0 61 67171.5 62 54955.5 63 41660.5 64 30607.0 65 23629.5 66 17533.5 67 13442.5 68 10821.5 69 8557.0 70 6700.5 71 4862.5 72 3652.0 73 3008.0 74 2343.5 75 1777.5 76 1312.0 77 829.0 78 541.0 79 385.5 80 285.0 81 207.5 82 145.5 83 99.0 84 67.0 85 51.0 86 41.5 87 27.5 88 20.0 89 17.5 90 13.0 91 11.5 92 9.0 93 5.5 94 5.0 95 3.5 96 3.5 97 5.0 98 3.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39070237493683674 2 0.1144685868283645 3 0.11568132053225534 4 0.12789848969737805 5 0.12987479647408906 6 0.07139408230868564 7 0.08080399752961653 8 0.07750266689124699 9 0.07707596429172983 10-11 0.07536915389366121 12-13 0.0793105384313065 14-15 0.07321318286452193 16-17 0.0749649093256976 18-19 0.0846106338779406 20-21 0.08321823592162148 22-23 0.09290887653697152 24-25 0.10617034416933356 26-27 0.10733816181011735 28-29 0.10155521868508224 30-31 0.09194318118016956 32-33 0.09018022570321711 34-35 0.33825164224355736 36-37 0.5292235135590365 38-39 0.5104935152433889 40-41 0.4827241592274437 42-43 0.5106956375273707 44-45 0.5197799112907754 46-47 0.5176127112458593 48-49 0.5247543652798832 50-51 0.5239795631912862 52-53 0.5216326988939419 54-55 0.5163662904946381 56-57 0.4877884453427657 58-59 0.4784234461849419 60-61 0.4278142720790522 62-63 0.5019145471899388 64-65 0.5279546347762618 66-67 0.538992757284824 68-69 0.5538599741732637 70-71 0.5498062994778508 72-73 0.5360282971197574 74-75 0.5398686205154118 76-77 0.5149851215540958 78-79 0.4889674919993262 80-81 0.49578350457582393 82-83 0.5095278198865869 84-85 0.503497838414463 86-87 0.5051148166863174 88-89 0.515647633484925 90-91 0.5339060131379484 92-93 0.5404974453989108 94-95 0.5351412048733928 96-97 0.5361181292459716 98-99 0.5368592442872382 100-101 0.5128179215091797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4452750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.655656302386326 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44415726468206 33.09406890814552 2 15.691301013008 14.95558496026391 3 6.022918145515661 8.61078351240301 4 2.9657659825543257 5.65342097351672 5 1.6567490002825973 3.94767304683948 6 1.0009482214932455 2.862050665198018 7 0.6582249006791536 2.1957697745506684 8 0.44787852120881977 1.707515589755885 9 0.33307051664485804 1.4285424659117036 >10 1.5972012522454722 13.535357517246105 >50 0.11627284868250046 3.812188206464567 >100 0.060307321677995886 5.045569284095111 >500 0.002933733936751701 0.9710637670840923 >1k 0.0021293225517613505 1.7182749818734042 >5k 1.4195483678405079E-4 0.4621363466516301 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7856 0.1764302958845657 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7630 0.17135478075346694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5008 0.11246982202009993 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.2458031553534332E-5 2.2458031553534332E-5 6 0.0 0.0 0.0 2.2458031553534332E-5 2.2458031553534332E-5 7 0.0 0.0 0.0 2.2458031553534332E-5 2.2458031553534332E-5 8 0.0 0.0 0.0 2.2458031553534332E-5 6.7374094660603E-5 9 0.0 0.0 0.0 2.2458031553534332E-5 6.7374094660603E-5 10-11 2.2458031553534332E-5 0.0 0.0 8.983212621413733E-5 8.983212621413733E-5 12-13 2.2458031553534332E-5 0.0 0.0 1.2351917354443882E-4 2.1335129975857617E-4 14-15 2.2458031553534332E-5 0.0 0.0 1.5720622087474033E-4 3.36870473303015E-4 16-17 2.2458031553534332E-5 0.0 0.0 1.908932682050418E-4 4.2670259951715233E-4 18-19 2.2458031553534332E-5 0.0 0.0 2.02122283981809E-4 4.940766941777553E-4 20-21 2.2458031553534332E-5 0.0 0.0 2.6949637864241195E-4 5.277637415080569E-4 22-23 2.2458031553534332E-5 0.0 0.0 3.705575206333165E-4 5.726798046151255E-4 24-25 2.2458031553534332E-5 0.0 0.0 6.400538992757284E-4 6.06366851945427E-4 26-27 2.2458031553534332E-5 0.0 0.0 0.0011678176407837852 6.06366851945427E-4 28-29 2.2458031553534332E-5 0.0 0.0 0.0035371399696816574 6.288248834989613E-4 30-31 2.2458031553534332E-5 0.0 0.0 0.011330076918758071 6.512829150524957E-4 32-33 2.2458031553534332E-5 0.0 0.0 0.028566616136095672 6.961989781595643E-4 34-35 2.2458031553534332E-5 0.0 0.0 0.05457301667508843 8.08489135927236E-4 36-37 2.2458031553534332E-5 0.0 0.0 0.09765875021054404 8.08489135927236E-4 38-39 2.2458031553534332E-5 0.0 0.0 0.16428050081410364 8.646342148110719E-4 40-41 2.2458031553534332E-5 0.0 0.0 0.2445230475548818 8.75863230587839E-4 42-43 2.2458031553534332E-5 0.0 0.0 0.3270675425298972 8.870922463646061E-4 44-45 2.2458031553534332E-5 0.0 0.0 0.42570321711302006 9.544663410252091E-4 46-47 2.2458031553534332E-5 0.0 0.0 0.5449104485991803 0.0010330694514625793 48-49 2.2458031553534332E-5 0.0 0.0 0.6736623434955926 0.0011229015776767167 50-51 2.2458031553534332E-5 0.0 0.0 0.8144292852731458 0.001145359609230251 52-53 2.2458031553534332E-5 0.0 0.0 0.9615855370276796 0.0012351917354443882 54-55 2.2458031553534332E-5 0.0 0.0 1.1231373870080286 0.0013250238616585256 56-57 2.2458031553534332E-5 0.0 0.0 1.3158160687215765 0.00134748189321206 58-59 2.2458031553534332E-5 0.0 0.0 1.526595923867273 0.0013811689405423613 60-61 2.2458031553534332E-5 0.0 0.0 1.761753972264331 0.0013923979563191286 62-63 2.2458031553534332E-5 0.0 0.0 2.004278255010948 0.001414855987872663 64-65 2.2458031553534332E-5 0.0 0.0 2.2603671888159003 0.0014597720509797315 66-67 2.2458031553534332E-5 0.0 0.0 2.5558924260288585 0.0014934590983100332 68-69 2.2458031553534332E-5 0.0 0.0 2.888832743810005 0.0015046881140868002 70-71 2.2458031553534332E-5 0.0 0.0 3.2554937959687837 0.0015383751614171017 72-73 2.2458031553534332E-5 0.0 0.0 3.6386951883667398 0.001762955476952445 74-75 2.2458031553534332E-5 0.0 0.0 4.042580427825501 0.0018415585873898153 76-77 2.2458031553534332E-5 0.0 0.0 4.473842007748021 0.001886474650496884 78-79 2.2458031553534332E-5 0.0 0.0 4.965414631407557 0.001886474650496884 80-81 2.2458031553534332E-5 0.0 0.0 5.498489697378025 0.001886474650496884 82-83 2.2458031553534332E-5 0.0 0.0 6.054112627028241 0.00202122283981809 84-85 2.2458031553534332E-5 0.0 0.0 6.627241592274437 0.00202122283981809 86-87 2.2458031553534332E-5 0.0 0.0 7.229768120824209 0.002088596934478693 88-89 2.2458031553534332E-5 0.0 0.0 7.869159508169108 0.002155971029139296 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 16425 0.0 24.864618 1 TACATGG 16140 0.0 24.562153 2 GTATCAA 21795 0.0 23.313936 1 ACATGGG 16455 0.0 22.767908 3 TCAACGC 24430 0.0 20.492552 4 ATCAACG 24550 0.0 20.373093 3 CAACGCA 24610 0.0 20.303492 5 TATCAAC 25040 0.0 20.050077 2 AACGCAG 25440 0.0 19.762283 6 GAGTACT 9490 0.0 19.206625 12-13 CATGGGG 8780 0.0 18.826752 4 AGAGTAC 18740 0.0 18.491669 10-11 AGTACTT 10275 0.0 18.130903 12-13 GTATTAG 1315 0.0 18.056475 1 GTAAGGT 1710 0.0 18.0037 4 ACGCAGA 27835 0.0 17.908815 7 CGCAGAG 28010 0.0 17.796724 8 TAAGGTG 1900 0.0 17.698425 5 GTATAGA 1250 0.0 17.475779 1 GAGTACA 13665 0.0 17.236963 1 >>END_MODULE