FastQCFastQC Report
Fri 27 May 2016
SRR939186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4452750
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84180.189051709617652No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81990.184133400707428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56110.12601201504688114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA231650.025.0977331
GTACATG167650.022.3137761
TACATGG166800.021.8491552
TCAACGC264850.021.7156124
ATCAACG266200.021.6233123
CAACGCA267750.021.533585
TATCAAC270700.021.3515032
AACGCAG276150.020.8785676
ACATGGG169200.020.4734923
GAGTACT111550.019.90972312-13
TATACCG2953.0595402E-919.3031715
AGTACTT120500.019.29736312-13
AGAGTAC213150.019.23600610-11
ACGCAGA301850.019.0537687
CGCAGAG303800.018.8846078
GTACTTT120150.018.32665314-15
CATGGGG90800.017.3508854
GCAGAGT330250.017.3148429
ACCGTAT3059.3963536E-817.1144248
GAGTACA148150.016.6623041
Produced by FastQC (version 0.11.5)