##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4452750 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.533849868059065 34.0 31.0 34.0 31.0 34.0 2 32.74192016169783 34.0 31.0 34.0 31.0 34.0 3 32.86041457526248 34.0 31.0 34.0 31.0 34.0 4 36.29447554881815 37.0 37.0 37.0 35.0 37.0 5 36.250255909269555 37.0 37.0 37.0 35.0 37.0 6 36.28644770085902 37.0 37.0 37.0 35.0 37.0 7 36.24599112907754 37.0 37.0 37.0 35.0 37.0 8 36.219209252709 37.0 37.0 37.0 35.0 37.0 9 37.978885407894 39.0 38.0 39.0 35.0 39.0 10-11 37.90110167873786 39.0 38.0 39.0 35.0 39.0 12-13 37.824606029981474 39.0 38.0 39.0 35.0 39.0 14-15 39.26553568019763 40.5 39.0 41.0 36.0 41.0 16-17 39.354338779405985 41.0 39.0 41.0 36.0 41.0 18-19 39.374140362697204 41.0 39.0 41.0 36.0 41.0 20-21 39.33391207680647 41.0 39.0 41.0 36.0 41.0 22-23 39.26733737577901 41.0 39.0 41.0 36.0 41.0 24-25 39.19962506316321 41.0 39.0 41.0 36.0 41.0 26-27 39.065456403346246 40.5 39.0 41.0 35.5 41.0 28-29 38.92786963112683 40.0 38.5 41.0 35.0 41.0 30-31 38.76332524844197 40.0 38.0 41.0 35.0 41.0 32-33 38.70581079108416 40.0 38.0 41.0 35.0 41.0 34-35 38.62179720397508 40.0 38.0 41.0 35.0 41.0 36-37 38.48793532086913 40.0 38.0 41.0 34.0 41.0 38-39 38.36970591207681 40.0 38.0 41.0 34.0 41.0 40-41 38.20553006568974 40.0 38.0 41.0 34.0 41.0 42-43 38.034190668687884 40.0 38.0 41.0 33.0 41.0 44-45 37.81033799337489 40.0 37.5 41.0 33.0 41.0 46-47 37.56279748470047 40.0 37.0 41.0 33.0 41.0 48-49 37.270225927797426 40.0 37.0 41.0 32.0 41.0 50-51 37.03011408680029 40.0 36.5 41.0 31.5 41.0 52-53 36.77226691370501 39.0 36.0 41.0 31.0 41.0 54-55 36.53569041603504 39.0 35.5 41.0 31.0 41.0 56-57 36.300791421031946 39.0 35.0 40.0 30.5 41.0 58-59 35.96864634214811 38.5 35.0 40.0 30.0 41.0 60-61 35.59363247431362 38.0 35.0 40.0 29.0 41.0 62-63 35.20636629049464 37.5 34.0 40.0 28.0 41.0 64-65 34.767562854415814 37.0 34.0 40.0 28.0 41.0 66-67 34.35019392510247 36.5 34.0 39.0 27.5 41.0 68-69 33.866965583066644 36.0 33.5 39.0 26.0 40.0 70-71 33.36770422772444 35.0 33.0 38.5 26.0 40.0 72-73 32.80939082589411 35.0 32.0 37.5 25.0 39.5 74-75 32.28191802818483 35.0 32.0 37.0 24.0 39.0 76-77 30.25662994778508 33.0 29.5 35.0 20.5 37.0 78-79 31.26615136713267 34.0 31.0 36.0 22.0 38.0 80-81 31.302708326315198 34.0 31.0 35.5 22.5 37.0 82-83 30.936329908483522 34.0 31.0 35.0 20.5 37.0 84-85 30.479180169558138 34.0 31.0 35.0 19.0 36.0 86-87 29.80294323732525 34.0 30.0 35.0 14.0 36.0 88-89 29.136032002694964 34.0 29.0 35.0 7.0 35.0 90-91 28.598289034866095 33.0 29.0 35.0 2.0 35.0 92-93 28.166207624501713 33.0 28.0 35.0 2.0 35.0 94-95 27.664065802032454 33.0 27.0 34.5 2.0 35.0 96-97 26.898814552804446 32.5 25.5 34.0 2.0 35.0 98-99 25.71553916119252 32.0 24.0 34.0 2.0 35.0 100-101 19.723941496827802 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19041592304917287 1101 2 0.14888374205773403 1101 3 0.1967329511819571 1101 4 0.14200661114367108 1101 5 0.16147157599352369 1101 6 0.18189894780004323 1101 7 0.19499127969705654 1101 8 0.21707828331315682 1101 9 0.22466903322278142 1101 10-11 0.2392886537170753 1101 12-13 0.25873337932879537 1101 14-15 0.31534974176033614 1101 16-17 0.32142511168716936 1101 18-19 0.3158708421607628 1101 20-21 0.2658963529635443 1101 22-23 0.2994271309861105 1101 24-25 0.21214150152565736 1101 26-27 0.22131138814192042 1101 28-29 0.208865173872681 1101 30-31 0.2636127669539121 1101 32-33 0.3082505478394566 1101 34-35 0.31174380033909443 1101 36-37 0.3268832946864393 1101 38-39 0.2843153863302419 1101 40-41 0.3776092801670998 1101 42-43 0.40095882253152837 1101 44-45 0.30720089383209626 1101 46-47 0.2532760027732621 1101 48-49 0.3019249100214054 1101 50-51 0.277695020303689 1101 52-53 0.1865255359731819 1101 54-55 0.28796710455377195 1101 56-57 0.34993133204593363 1101 58-59 0.28386939005471845 1101 60-61 0.258035076787813 1101 62-63 0.2773968829254514 1101 64-65 0.23252352395474674 1101 66-67 0.21628136717134794 1101 68-69 0.2609391086884898 1101 70-71 0.27454135545649905 1101 72-73 0.3780431955442296 1101 74-75 0.15660121618134326 1101 76-77 0.240583303553759 1101 78-79 0.24011111040654143 1101 80-81 0.4255732594590498 1101 82-83 0.29407436902969764 1101 84-85 0.3993133924281409 1101 86-87 0.5107182127955348 1101 88-89 0.57089618510539 1101 90-91 0.6938338897724172 1101 92-93 0.6236015768667578 1101 94-95 0.435559957817393 1101 96-97 0.47381085050058047 1101 98-99 0.4002672048443081 1101 100-101 -0.6574091899871917 1102 1 0.06547266331493518 1102 2 0.08675297538466253 1102 3 0.08096390732183778 1102 4 0.05566113677537032 1102 5 0.07612718963299869 1102 6 0.11058180913400406 1102 7 0.13421159888160616 1102 8 0.12861434173448316 1102 9 0.14016201373167547 1102 10-11 0.15897633843035663 1102 12-13 0.17860252749262884 1102 14-15 0.2354534749114663 1102 16-17 0.22264273319706263 1102 18-19 0.19615798093080627 1102 20-21 0.19762028174246637 1102 22-23 0.148245310300247 1102 24-25 0.160429020578448 1102 26-27 0.22894086345572617 1102 28-29 0.2687935750352821 1102 30-31 0.1816355201166786 1102 32-33 0.1820196529720377 1102 34-35 0.20805045523096055 1102 36-37 0.2439183456254952 1102 38-39 0.22446719447211905 1102 40-41 0.24835551689971425 1102 42-43 0.24110628117190913 1102 44-45 0.31465923488304526 1102 46-47 0.17253428839792662 1102 48-49 0.157681352272526 1102 50-51 0.10329511800234314 1102 52-53 0.1924283154100337 1102 54-55 0.22802953360208278 1102 56-57 0.10714026186057879 1102 58-59 0.081171448387785 1102 60-61 0.05652548357230103 1102 62-63 0.05240135188012829 1102 64-65 0.20102094193888576 1102 66-67 0.24822385312997852 1102 68-69 0.2597645676206213 1102 70-71 0.10355707662071012 1102 72-73 0.11269454962344128 1102 74-75 0.013036644423848998 1102 76-77 0.1440601713730416 1102 78-79 0.1817545727052874 1102 80-81 0.2373902858390906 1102 82-83 0.11659830112519742 1102 84-85 0.03768027477574165 1102 86-87 0.2741147476411534 1102 88-89 0.2405918802091307 1102 90-91 0.21456228581961412 1102 92-93 0.12574252938369312 1102 94-95 0.12556322240283535 1102 96-97 0.026562995809626955 1102 98-99 0.04906294803850031 1102 100-101 -0.23504389172943618 1103 1 0.1271429608398833 1103 2 0.08971768718402728 1103 3 0.13925018807017864 1103 4 0.11939563088282057 1103 5 0.16050461253618664 1103 6 0.15621272830884436 1103 7 0.13996507396790037 1103 8 0.12670683424944684 1103 9 0.23259386154047945 1103 10-11 0.14401837623053382 1103 12-13 0.14780624147821442 1103 14-15 0.12240304771012234 1103 16-17 0.2170806141551722 1103 18-19 0.250545674604858 1103 20-21 0.29174558239568427 1103 22-23 0.23395018837990023 1103 24-25 0.25297394866654344 1103 26-27 0.26042312856102257 1103 28-29 0.24837845760512778 1103 30-31 0.31069361641705484 1103 32-33 0.33434635538051083 1103 34-35 0.37423836494061646 1103 36-37 0.4333318028940809 1103 38-39 0.4076314530699321 1103 40-41 0.4443764667410477 1103 42-43 0.426297661978424 1103 44-45 0.432601622269118 1103 46-47 0.5961224219570127 1103 48-49 0.5159227723407369 1103 50-51 0.5438301610581391 1103 52-53 0.62493967984053 1103 54-55 0.585215125028455 1103 56-57 0.48511095783531033 1103 58-59 0.5564457987539626 1103 60-61 0.6480837793451855 1103 62-63 0.562088768823898 1103 64-65 0.660275929989794 1103 66-67 0.6205397097364767 1103 68-69 0.5263000822569524 1103 70-71 0.5575872605261125 1103 72-73 0.5604391483205191 1103 74-75 0.561620375830941 1103 76-77 0.44062028853631574 1103 78-79 0.544079745365945 1103 80-81 0.693006688917734 1103 82-83 0.5993272144044504 1103 84-85 0.4290864074136813 1103 86-87 0.4287192885709885 1103 88-89 0.6830084305397577 1103 90-91 0.9462297301998 1103 92-93 0.9681644723505087 1103 94-95 0.8602420138800504 1103 96-97 0.6379994840368965 1103 98-99 0.6011211466889073 1103 100-101 -0.2446059849086879 1104 1 0.11293437321260313 1104 2 0.08919553023653748 1104 3 0.09911775749404228 1104 4 0.057683650550643506 1104 5 0.07291797985926252 1104 6 0.057168762167762566 1104 7 0.0932436014274387 1104 8 0.06380941296822584 1104 9 0.07271676965742557 1104 10-11 0.1294856435662055 1104 12-13 0.12953574629192133 1104 14-15 0.02783897773741728 1104 16-17 0.08171668654585318 1104 18-19 0.1718508354883852 1104 20-21 0.15274398330647188 1104 22-23 0.14457921257246653 1104 24-25 0.20170925034305043 1104 26-27 0.16937112304430713 1104 28-29 0.17610069062707367 1104 30-31 0.25050753125523073 1104 32-33 0.23694948532340732 1104 34-35 0.2590412697641611 1104 36-37 0.3182928015843487 1104 38-39 0.24316628514715433 1104 40-41 0.2252365999660313 1104 42-43 0.29776971579661904 1104 44-45 0.2815312268319019 1104 46-47 0.18814377173140429 1104 48-49 0.16013929885802014 1104 50-51 0.12964336723594982 1104 52-53 0.09542821093280196 1104 54-55 0.11562077126804127 1104 56-57 0.14941369154394835 1104 58-59 0.12053497421685222 1104 60-61 0.09100669819463292 1104 62-63 0.04494235785718814 1104 64-65 0.09645250216227197 1104 66-67 0.03368067711837597 1104 68-69 0.11938402799577119 1104 70-71 -0.021754356119139118 1104 72-73 -0.14934878230442195 1104 74-75 -0.1116539217627377 1104 76-77 -0.17430224678424722 1104 78-79 0.1319367948398522 1104 80-81 0.19401371528258338 1104 82-83 0.12735125940307057 1104 84-85 -0.05265434284562076 1104 86-87 -0.051681478065891184 1104 88-89 0.23319198777802796 1104 90-91 0.21333714380255486 1104 92-93 0.02172679276863576 1104 94-95 0.06046131979799796 1104 96-97 -0.0831158326808854 1104 98-99 -0.10972004157721216 1104 100-101 -0.47555212277548264 1105 1 0.010571338190125346 1105 2 0.03660939965121912 1105 3 0.04621603564717702 1105 4 0.056028206996337815 1105 5 0.058571554692008476 1105 6 0.08118930707112071 1105 7 0.07290746225332612 1105 8 0.03616326738197273 1105 9 0.13705876877322254 1105 10-11 0.10942710433371161 1105 12-13 0.03948835619529234 1105 14-15 0.13585469485931156 1105 16-17 0.11451216058849667 1105 18-19 0.1651004173957915 1105 20-21 0.14832664602405288 1105 22-23 0.17583898467971437 1105 24-25 0.17500730817160814 1105 26-27 0.22835609528306122 1105 28-29 0.16896214669016985 1105 30-31 0.232412190345201 1105 32-33 0.2552382935820958 1105 34-35 0.22223996837965387 1105 36-37 0.3169962694270154 1105 38-39 0.28994306645733303 1105 40-41 0.36129223079372963 1105 42-43 0.3112054150488106 1105 44-45 0.34010268298778357 1105 46-47 0.3615757144821288 1105 48-49 0.2546583801551847 1105 50-51 0.29929160021327306 1105 52-53 0.3345093425876513 1105 54-55 0.2840754122651177 1105 56-57 0.3374535475731406 1105 58-59 0.2906076316758188 1105 60-61 0.2788423038602801 1105 62-63 0.30257478895308765 1105 64-65 0.41151132100085164 1105 66-67 0.34675184777513124 1105 68-69 0.28872764080227853 1105 70-71 0.2808255679634115 1105 72-73 0.10188787904345276 1105 74-75 0.1631932164627159 1105 76-77 0.13399091981860067 1105 78-79 0.10751589916251447 1105 80-81 0.31499619589259 1105 82-83 0.36705448324867973 1105 84-85 0.24871148899000062 1105 86-87 0.28108737697603203 1105 88-89 0.2688767455852421 1105 90-91 0.4828396681564975 1105 92-93 0.514144264462594 1105 94-95 0.39307407189171784 1105 96-97 0.0737015529495686 1105 98-99 -0.13505404546361177 1105 100-101 -0.8159799902988745 1106 1 0.019543445939206094 1106 2 0.006964406433915826 1106 3 0.043864049955701034 1106 4 0.0070392561638144 1106 5 0.07649351427860296 1106 6 0.04898500303965392 1106 7 0.0913826062086045 1106 8 0.07676043038403435 1106 9 0.13490482498998801 1106 10-11 0.11237000232951999 1106 12-13 0.14743476396024846 1106 14-15 0.08953306147058981 1106 16-17 0.0247169959054645 1106 18-19 -0.011103026391133142 1106 20-21 0.08763763156579785 1106 22-23 0.15670259021832322 1106 24-25 0.15301334987798754 1106 26-27 0.12072317452909687 1106 28-29 0.20949629094798894 1106 30-31 0.17908851393519853 1106 32-33 0.16836912668394177 1106 34-35 0.04115093545018311 1106 36-37 0.08871458766083862 1106 38-39 0.2912711612013439 1106 40-41 0.2528297120100902 1106 42-43 0.1818494749460129 1106 44-45 0.24285220448568623 1106 46-47 0.2250846719509596 1106 48-49 0.15065787410097897 1106 50-51 0.19523842976941097 1106 52-53 0.30813864622040654 1106 54-55 0.14511158323907125 1106 56-57 0.12501515895826998 1106 58-59 0.09068146893535811 1106 60-61 0.11646168540301005 1106 62-63 0.1381397237289903 1106 64-65 0.16720478783157233 1106 66-67 0.22075393197579274 1106 68-69 0.11479013444981945 1106 70-71 -0.0310994231877757 1106 72-73 0.04651963415892624 1106 74-75 -0.011969376658043984 1106 76-77 0.025700438041191376 1106 78-79 0.13717933979314267 1106 80-81 0.36534966023350535 1106 82-83 0.22577145732066484 1106 84-85 0.18165085698400318 1106 86-87 0.18390717716793503 1106 88-89 0.19201958497777838 1106 90-91 0.32352685453559005 1106 92-93 0.4127868561837289 1106 94-95 0.24675210525940017 1106 96-97 -0.13373603945114354 1106 98-99 0.042798240028080414 1106 100-101 -0.5446602552084876 1107 1 0.004993603826186188 1107 2 0.023302114620499026 1107 3 0.0561398726754021 1107 4 -0.017448855311094746 1107 5 -0.045441479755069736 1107 6 0.05708304441053258 1107 7 0.019173829313032797 1107 8 0.04905228668397399 1107 9 0.040685196989812766 1107 10-11 0.09588559049490897 1107 12-13 0.10122695169758344 1107 14-15 0.11091511816997013 1107 16-17 0.06177078265388758 1107 18-19 0.09248819991820056 1107 20-21 0.06437878381236573 1107 22-23 0.09144068245248604 1107 24-25 0.12746079058771187 1107 26-27 0.09606121923128086 1107 28-29 0.1095671262401865 1107 30-31 0.12665595303795385 1107 32-33 0.06761780087793312 1107 34-35 0.018101196819664267 1107 36-37 0.1619528856186605 1107 38-39 0.07744348472305518 1107 40-41 0.14000134692745547 1107 42-43 0.06748004446537692 1107 44-45 0.06349390429797808 1107 46-47 0.18673549199049688 1107 48-49 0.06889756122949109 1107 50-51 0.1501592763803572 1107 52-53 0.16398369195922413 1107 54-55 0.003560675154780313 1107 56-57 0.0444242998594504 1107 58-59 -0.023246101442325084 1107 60-61 -0.07314696794552589 1107 62-63 -0.05285262714280492 1107 64-65 -0.148869519597433 1107 66-67 -0.11241061692967236 1107 68-69 -0.17967399210712642 1107 70-71 -0.15035684994710863 1107 72-73 -0.2607993431315734 1107 74-75 -0.2845673244771305 1107 76-77 -0.23285193324272058 1107 78-79 -0.06497817433385222 1107 80-81 0.0414710008520629 1107 82-83 -0.03870638715586594 1107 84-85 -0.05269529952712304 1107 86-87 -0.0418980622287215 1107 88-89 -0.28464325009053226 1107 90-91 0.0769031261458828 1107 92-93 0.12123316073012091 1107 94-95 0.18368059580750895 1107 96-97 0.12018760603971756 1107 98-99 0.008757294814220273 1107 100-101 -0.6992986521574132 1108 1 -0.08203357477076878 1108 2 -0.01734663880679932 1108 3 -0.017730663882630893 1108 4 0.016568058184866175 1108 5 -0.02714479674433079 1108 6 4.2480869132077714E-4 1108 7 -0.022851814821812866 1108 8 0.05050934720170375 1108 9 0.041554689331995576 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-0.944747932297723 2316 90-91 -1.109226489395791 2316 92-93 -1.1801799499360754 2316 94-95 -1.0629503133921254 2316 96-97 -1.0704309594469414 2316 98-99 -0.8517929007454192 2316 100-101 0.47929977473148355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 15.0 4 25.0 5 68.0 6 132.0 7 314.0 8 841.0 9 1565.0 10 2937.0 11 4501.0 12 6909.0 13 9512.0 14 11961.0 15 15720.0 16 20397.0 17 28087.0 18 27677.0 19 20786.0 20 20539.0 21 21872.0 22 23409.0 23 26398.0 24 30387.0 25 35995.0 26 42734.0 27 52099.0 28 63328.0 29 78592.0 30 96032.0 31 118920.0 32 151354.0 33 200430.0 34 283164.0 35 434049.0 36 725239.0 37 1135967.0 38 730017.0 39 30776.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61065100501606 19.146999386525206 14.477463823030565 23.76488578542817 2 15.473359160069618 20.544607265173205 39.98515524114311 23.996878333614056 3 19.894806580203245 24.221391275054742 28.982202009993824 26.90160013474819 4 13.518657009713097 16.415922744371457 36.66015383751614 33.40526640839931 5 14.302464768963 36.96082196395486 33.78011341305934 14.956599854022794 6 32.47808657571164 35.541721408118576 17.387591937566672 14.59260007860311 7 29.004143506821627 32.00882600640054 20.770243108191565 18.216787378586265 8 25.78107910841615 35.0354275447757 20.044332154286675 19.139161192521474 9 26.163876256246137 17.512840379540734 20.601538375161418 35.72174498905171 10-11 25.115456740216718 26.360238055134467 27.124013250238615 21.400291954410196 12-13 26.093605075515132 23.61680983661782 27.485677390376733 22.803907697490313 14-15 22.858091234867526 25.136453219063014 25.97678293688808 26.028672609181385 16-17 22.492911790206282 28.205443489093717 26.637205496379057 22.66443922432094 18-19 22.432317107405535 27.24244567963618 28.36068721576554 21.964549997192744 20-21 23.20659143226096 26.27777216326989 28.34240637808096 22.17323002638819 22-23 22.78599741732637 26.24493852113862 28.197653135702655 22.77141092583235 24-25 22.768139723139537 26.377517167205827 28.369904565685665 22.484438543968967 26-27 22.515072375137265 26.565189615639135 28.33240447518354 22.58733353404006 28-29 22.36094178685367 27.065002660716186 28.1771247578883 22.396930794541845 30-31 22.637549828757507 26.565010386839592 28.01073493908259 22.78670484532031 32-33 22.381850221273446 26.600547953696463 28.08760701396003 22.929994811070063 34-35 23.03647293868993 26.581862245786077 27.945489931393546 22.43617488413045 36-37 23.02587456454994 26.30226734485398 28.020839826825238 22.651018263770837 38-39 22.633635639420106 26.663367652144306 28.005432367263428 22.69756434117216 40-41 22.94575771014307 26.51322089347786 27.924279801377494 22.616741595001578 42-43 22.70456360632438 26.564306627771906 27.659737900563208 23.07139186534051 44-45 22.760497009661457 26.622013998820236 27.747561278667167 22.86992771285114 46-47 23.117041293974594 26.8037899487795 27.293502052997475 22.78566670424843 48-49 22.91000624577635 26.76723916247755 27.362036504889602 22.960718086856495 50-51 22.810135292645974 26.941656758860542 27.447471409597462 22.800736538896018 52-53 23.103318174162034 26.88172477682331 27.150558643534893 22.86439840547976 54-55 22.787008028746282 26.78826567851328 27.35882319914659 23.065903093593846 56-57 22.851057369516532 26.909573593654972 27.209433365642973 23.029935671185527 58-59 22.958099312470782 26.61680310311748 27.262189194448695 23.16290838996304 60-61 22.694520984502912 26.808695419776523 27.568154464662896 22.928629131057665 62-63 22.607835692975154 26.75830568430736 27.59341912195295 23.04043950076454 64-65 22.426049323658155 26.972104127223684 27.366601312033563 23.2352452370846 66-67 22.512821854573616 26.837683119821975 27.16616155564836 23.483333469956047 68-69 22.684998182502365 26.616677340324387 27.39153660357137 23.306787873601884 70-71 22.738459392853105 26.563167951810396 27.16430282492068 23.534069830415827 72-73 22.688035483689855 26.524484868901244 27.30311605187805 23.48436359553085 74-75 22.470900005614507 27.005996294424794 26.98335859861883 23.539745101341865 76-77 22.55035903096203 26.622433857319404 27.188835617939578 23.63837149377899 78-79 22.61071965247158 26.659486389293825 27.192077286439158 23.537716671795437 80-81 22.67327069544762 26.686499414461196 27.0167794568576 23.623450433233586 82-83 22.658480714165403 26.6448037729493 27.204278255010948 23.492437257874347 84-85 22.53535455617315 26.678692942563586 27.079074729099993 23.70687777216327 86-87 22.466291524774405 26.677813457400397 27.08489164703388 23.771003370791316 88-89 22.494557823742955 26.648248670241557 26.873727978694994 23.98346552732049 90-91 22.40776420928225 26.446510825381413 27.037329620839586 24.108395344496753 92-93 22.490258828813655 26.43820111167256 27.073909381842682 23.997630677671104 94-95 22.309942085202216 26.278569997673344 27.202055898963966 24.20943201816047 96-97 22.219204111976207 26.04261703920241 27.38527060639724 24.35290824242415 98-99 22.17681950725216 26.27163160514056 26.958140867743573 24.59340801986371 100-101 22.534811450259532 25.26952240054573 27.6369873589 24.558678790294735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 294.0 1 201.0 2 166.5 3 238.0 4 216.5 5 210.5 6 379.0 7 751.5 8 1220.5 9 1443.0 10 1353.5 11 1312.5 12 1434.0 13 1619.0 14 1884.5 15 2142.0 16 2389.0 17 2722.0 18 3054.5 19 3434.5 20 4017.5 21 4795.5 22 5820.5 23 7325.5 24 9281.0 25 12283.5 26 17114.0 27 23332.5 28 28837.0 29 35299.5 30 43428.5 31 52959.5 32 65714.0 33 75137.0 34 82207.5 35 91619.5 36 102817.5 37 113426.5 38 121028.0 39 126834.0 40 133232.0 41 140296.5 42 144479.0 43 149641.5 44 156136.5 45 162399.5 46 167298.5 47 175983.5 48 191283.5 49 190605.5 50 176273.0 51 167134.0 52 160846.0 53 157286.0 54 154656.5 55 144753.5 56 131679.5 57 119627.0 58 109886.0 59 99132.5 60 82433.5 61 67194.5 62 54925.0 63 41271.5 64 30046.5 65 23106.5 66 16887.0 67 12875.0 68 10201.0 69 7870.0 70 6109.5 71 4489.5 72 3394.5 73 2806.0 74 2190.0 75 1629.0 76 1163.5 77 725.5 78 475.5 79 306.5 80 170.5 81 102.0 82 67.0 83 35.5 84 20.0 85 9.5 86 5.5 87 4.5 88 4.0 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.426478019201617 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.523440570434001E-4 16-17 0.0024591544551120096 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.3687047330301494E-5 26-27 0.0033799337488069165 28-29 3.3687047330301494E-5 30-31 0.0 32-33 3.3687047330301494E-5 34-35 0.012239627196676211 36-37 0.026444332154286678 38-39 0.02056032788726068 40-41 0.008635113132333951 42-43 0.008848464432092527 44-45 0.0036045140643422608 46-47 0.005300095446634102 48-49 0.003166582449048341 50-51 7.186570097130986E-4 52-53 0.0 54-55 0.0 56-57 4.4916063107068665E-5 58-59 0.0014934590983100332 60-61 0.002403009376228174 62-63 0.0013811689405423616 64-65 0.0026949637864241197 66-67 0.0022121161080231318 68-69 0.004480377294930099 70-71 1.2351917354443882E-4 72-73 0.0 74-75 0.0 76-77 0.09696255123238448 78-79 0.016618943349615407 80-81 3.031834259727135E-4 82-83 0.0 84-85 0.0 86-87 0.10853966649823142 88-89 0.4236033911627646 90-91 0.4283308068047836 92-93 0.0 94-95 4.4916063107068665E-5 96-97 1.1229015776767166E-5 98-99 1.3474818932120597E-4 100-101 2.807253944191792E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4452750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.441722722163966 #Duplication Level Percentage of deduplicated Percentage of total 1 68.29982677251982 31.719616169411964 2 16.047159207281428 14.90515436765969 3 6.344111102431932 8.83894346213237 4 3.105514957511762 5.769018582651762 5 1.7501291432099375 4.0639506198467155 6 1.0727794326885292 2.9893034972976618 7 0.7056003852624769 2.293850821150841 8 0.47080711827434146 1.7492074914014415 9 0.3522428778546627 1.4722889457764983 >10 1.662064673777497 13.851998374486548 >50 0.12269158391565087 3.9647294774421122 >100 0.061543493729254134 5.108387381944886 >500 0.0032496480958993414 1.0560518144270967 >1k 0.0021340968438473933 1.7168075661936608 >5k 1.455066029895752E-4 0.5006914281767793 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8418 0.189051709617652 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8199 0.184133400707428 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5611 0.12601201504688114 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.7374094660603E-5 2 0.0 0.0 0.0 2.2458031553534332E-5 6.7374094660603E-5 3 0.0 0.0 0.0 2.2458031553534332E-5 6.7374094660603E-5 4 0.0 0.0 0.0 6.7374094660603E-5 6.7374094660603E-5 5 0.0 0.0 0.0 6.7374094660603E-5 6.7374094660603E-5 6 0.0 0.0 0.0 6.7374094660603E-5 6.7374094660603E-5 7 0.0 0.0 0.0 8.983212621413733E-5 8.983212621413733E-5 8 0.0 0.0 0.0 8.983212621413733E-5 1.1229015776767166E-4 9 0.0 0.0 0.0 8.983212621413733E-5 1.1229015776767166E-4 10-11 0.0 0.0 0.0 1.5720622087474033E-4 1.1229015776767166E-4 12-13 0.0 0.0 0.0 1.684352366515075E-4 3.817865364100836E-4 14-15 0.0 0.0 0.0 1.908932682050418E-4 5.277637415080569E-4 16-17 0.0 0.0 0.0 2.3580933131211047E-4 5.726798046151255E-4 18-19 0.0 0.0 0.0 2.4703834708887765E-4 5.951378361686598E-4 20-21 0.0 0.0 0.0 2.807253944191792E-4 7.186570097130986E-4 22-23 0.0 0.0 0.0 4.04244567963618E-4 7.41115041266633E-4 24-25 0.0 0.0 0.0 7.074279939363315E-4 7.748020885969345E-4 26-27 0.0 0.0 0.0 0.0012464207512211554 8.534051990343047E-4 28-29 0.0 0.0 0.0 0.003615743080119027 8.75863230587839E-4 30-31 0.0 0.0 0.0 0.011622031328954018 8.75863230587839E-4 32-33 0.0 0.0 0.0 0.029116837909157263 9.320083094716749E-4 34-35 0.0 0.0 0.0 0.055257986637471224 9.881533883555106E-4 36-37 0.0 0.0 0.0 0.09912975127730055 0.001010611419909045 38-39 0.0 0.0 0.0 0.16633541070125202 0.0010555274830161135 40-41 0.0 0.0 0.0 0.24753242378305543 0.0010555274830161135 42-43 0.0 0.0 0.0 0.3307731177362304 0.0011116725618999495 44-45 0.0 0.0 0.0 0.430430632755039 0.0011229015776767167 46-47 0.0 0.0 0.0 0.5508730559766437 0.001134130593453484 48-49 0.0 0.0 0.0 0.680680478356072 0.0011790466565605525 50-51 0.0 0.0 0.0 0.822424344506204 0.0012576497669979226 52-53 0.0 0.0 0.0 0.9708494750435124 0.0013025658301049914 54-55 0.0 0.0 0.0 1.1343214867216889 0.0013025658301049914 56-57 0.0 0.0 0.0 1.3291673684801526 0.0013250238616585256 58-59 0.0 0.0 0.0 1.5420021335129976 0.00134748189321206 60-61 0.0 0.0 0.0 1.7794733591600695 0.0013699399247655943 62-63 0.0 0.0 0.0 2.0244680253775758 0.001414855987872663 64-65 0.0 0.0 0.0 2.2830722587165235 0.0014373140194261973 66-67 0.0 0.0 0.0 2.5810454213688168 0.0014710010667564988 68-69 0.0 0.0 0.0 2.916467351636629 0.0015271461456403347 70-71 0.0 0.0 0.0 3.285643703329403 0.0015720622087474032 72-73 0.0 0.0 0.0 3.671854471955533 0.0017404974453989108 74-75 2.2458031553534332E-5 0.0 0.0 4.0788501487844595 0.0018415585873898153 76-77 2.2458031553534332E-5 0.0 0.0 4.513177250014037 0.0018640166189433497 78-79 2.2458031553534332E-5 0.0 0.0 5.006883386671158 0.001875245634720117 80-81 2.2458031553534332E-5 0.0 0.0 5.54150805681882 0.001897703666273651 82-83 2.2458031553534332E-5 0.0 0.0 6.099556453876818 0.001976306776711021 84-85 2.2458031553534332E-5 0.0 0.0 6.6745269777104035 0.0019987648082645556 86-87 2.2458031553534332E-5 0.0 0.0 7.279669866936163 0.0020661389029251586 88-89 2.2458031553534332E-5 0.0 0.0 7.920521026332041 0.002111054966032227 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 23165 0.0 25.097733 1 GTACATG 16765 0.0 22.313776 1 TACATGG 16680 0.0 21.849155 2 TCAACGC 26485 0.0 21.715612 4 ATCAACG 26620 0.0 21.623312 3 CAACGCA 26775 0.0 21.53358 5 TATCAAC 27070 0.0 21.351503 2 AACGCAG 27615 0.0 20.878567 6 ACATGGG 16920 0.0 20.473492 3 GAGTACT 11155 0.0 19.909723 12-13 TATACCG 295 3.0595402E-9 19.303171 5 AGTACTT 12050 0.0 19.297363 12-13 AGAGTAC 21315 0.0 19.236006 10-11 ACGCAGA 30185 0.0 19.053768 7 CGCAGAG 30380 0.0 18.884607 8 GTACTTT 12015 0.0 18.326653 14-15 CATGGGG 9080 0.0 17.350885 4 GCAGAGT 33025 0.0 17.314842 9 ACCGTAT 305 9.3963536E-8 17.114424 8 GAGTACA 14815 0.0 16.662304 1 >>END_MODULE