##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1213407 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.211242394349135 33.0 31.0 34.0 31.0 34.0 2 32.29677593750489 34.0 31.0 34.0 31.0 34.0 3 32.3292052872614 34.0 31.0 34.0 30.0 34.0 4 35.777917879161734 37.0 35.0 37.0 35.0 37.0 5 35.84564371229109 37.0 35.0 37.0 35.0 37.0 6 35.96414311109133 37.0 37.0 37.0 35.0 37.0 7 35.91314950383507 37.0 37.0 37.0 35.0 37.0 8 35.91436921000126 37.0 37.0 37.0 35.0 37.0 9 37.59332688866967 39.0 38.0 39.0 35.0 39.0 10-11 37.525987570534866 39.0 38.0 39.0 35.0 39.0 12-13 37.468787059906525 39.0 38.0 39.0 35.0 39.0 14-15 38.724230204704604 40.0 38.0 41.0 35.0 41.0 16-17 38.70377128201832 40.0 38.0 41.0 35.0 41.0 18-19 38.74599124613588 40.0 38.5 41.0 35.0 41.0 20-21 38.69567342202575 40.0 38.5 41.0 35.0 41.0 22-23 38.60606045621955 40.0 38.0 41.0 34.5 41.0 24-25 38.615489279359686 40.5 38.0 41.0 35.0 41.0 26-27 38.50945066247351 40.0 38.0 41.0 34.5 41.0 28-29 38.400056617441635 40.0 38.0 41.0 34.0 41.0 30-31 38.195757070793235 40.0 38.0 41.0 34.0 41.0 32-33 37.9088599291087 40.0 37.0 41.0 34.0 41.0 34-35 37.566446789906436 40.0 37.0 41.0 33.0 41.0 36-37 37.28857506178883 40.0 36.5 41.0 33.0 41.0 38-39 37.0406149791455 40.0 36.0 41.0 32.5 41.0 40-41 36.90731963801099 40.0 36.0 41.0 31.5 41.0 42-43 36.641754580285095 40.0 36.0 41.0 31.0 41.0 44-45 36.246309358690034 40.0 35.5 41.0 30.0 41.0 46-47 35.69629934556171 40.0 35.0 41.0 28.0 41.0 48-49 35.438043459449304 40.0 35.5 41.0 24.5 41.0 50-51 34.398512617777875 38.5 34.0 40.0 23.0 40.5 52-53 34.57780406739042 38.5 35.0 40.0 23.0 40.5 54-55 35.10545389963961 40.0 35.0 41.0 23.0 41.0 56-57 34.99825779808424 40.0 35.0 41.0 22.0 41.0 58-59 34.8542661283477 39.0 35.0 41.0 20.5 41.0 60-61 34.56645544322721 39.0 35.0 41.0 18.5 41.0 62-63 34.25392716541111 39.0 35.0 41.0 13.5 41.0 64-65 33.920190834567464 38.5 35.0 40.5 9.0 41.0 66-67 33.50569553332064 38.0 34.0 40.0 6.5 41.0 68-69 33.08411645886335 37.0 34.0 40.0 2.0 41.0 70-71 32.642588595582524 36.5 33.5 39.0 2.0 41.0 72-73 32.16845625581524 36.0 33.0 39.0 2.0 40.5 74-75 31.69436677058893 35.5 33.0 38.5 2.0 40.0 76-77 31.241665821937737 35.0 32.0 37.0 2.0 39.5 78-79 30.771552743638367 35.0 32.0 37.0 2.0 39.0 80-81 30.287704784956738 35.0 31.0 36.0 2.0 39.0 82-83 29.81996230448646 35.0 31.0 36.0 2.0 37.5 84-85 29.397613084480312 35.0 30.5 35.5 2.0 37.0 86-87 29.04522143023734 34.5 30.0 35.0 2.0 36.5 88-89 28.602558745746478 34.0 29.5 35.0 2.0 36.0 90-91 28.19250507043391 34.0 29.0 35.0 2.0 36.0 92-93 27.78129926726976 34.0 29.0 35.0 2.0 35.5 94-95 27.324740997867988 34.0 27.0 35.0 2.0 35.0 96-97 26.790650622585826 34.0 25.5 35.0 2.0 35.0 98-99 26.207691648391677 34.0 24.5 35.0 2.0 35.0 100-101 23.866241500172656 31.5 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18274700757370255 1101 2 0.3000553433855444 1101 3 0.24901438977308743 1101 4 0.0829389687012494 1101 5 0.1745864730048936 1101 6 0.21603859679515836 1101 7 0.13698210760098561 1101 8 0.2764429060504128 1101 9 0.37845145073646336 1101 10-11 0.35173412121167047 1101 12-13 0.30332721218368164 1101 14-15 0.23031824608354157 1101 16-17 0.30888261740987843 1101 18-19 0.37850128025739593 1101 20-21 0.4770223839511729 1101 22-23 0.5384687991106532 1101 24-25 0.48935471201247793 1101 26-27 0.520187345123297 1101 28-29 0.4871519660319734 1101 30-31 0.3615472103909454 1101 32-33 0.38567397830705374 1101 34-35 0.39584198264064696 1101 36-37 0.4284383040094184 1101 38-39 0.4366846558880866 1101 40-41 0.21062562669020934 1101 42-43 0.11498733347468004 1101 44-45 0.23499915341320587 1101 46-47 0.17423279293306138 1101 48-49 0.320759275501608 1101 50-51 0.21230813470132404 1101 52-53 0.17112342572755068 1101 54-55 0.23969291826069394 1101 56-57 0.12908946805175958 1101 58-59 0.2541976723531221 1101 60-61 0.22249434433432924 1101 62-63 0.2472809707048924 1101 64-65 0.19714991654214487 1101 66-67 0.04256385722725753 1101 68-69 -0.0022150187906575525 1101 70-71 0.10335370287548074 1101 72-73 0.003963755519535539 1101 74-75 -0.02469174156115983 1101 76-77 -0.13816577638044336 1101 78-79 -0.14261766557266142 1101 80-81 -0.2331883958931087 1101 82-83 -0.22986157334093704 1101 84-85 -0.290636890338309 1101 86-87 -0.3076549909972215 1101 88-89 -0.3852854992888233 1101 90-91 -0.4072179979540529 1101 92-93 -0.38341402449669104 1101 94-95 -0.34320638183000085 1101 96-97 -0.32409577317627836 1101 98-99 -0.4456593295319209 1101 100-101 -0.6457095236154267 1102 1 0.16619039249871292 1102 2 0.16116129000493373 1102 3 0.23904841152784684 1102 4 0.15893302687365463 1102 5 0.1742845898288934 1102 6 0.2565743196376573 1102 7 0.23700031642747632 1102 8 0.22325971567234149 1102 9 0.18715143636107712 1102 10-11 0.14743971323812843 1102 12-13 0.24317195797888047 1102 14-15 0.23513927248634303 1102 16-17 0.2508554000060741 1102 18-19 0.36610442367586415 1102 20-21 0.3735315602413678 1102 22-23 0.3600639881470258 1102 24-25 0.17604597656755772 1102 26-27 0.10253724209967885 1102 28-29 0.2534852833926493 1102 30-31 0.20333215456651743 1102 32-33 0.09259764498968792 1102 34-35 0.02596513180927218 1102 36-37 0.12656998257570962 1102 38-39 0.08525382546976346 1102 40-41 0.09138608478595245 1102 42-43 -0.08956487314758022 1102 44-45 -0.07919750191287989 1102 46-47 -0.1368841748181424 1102 48-49 -0.08903467725128422 1102 50-51 -0.026814008190036986 1102 52-53 -0.1577251055868416 1102 54-55 -0.016652378591096806 1102 56-57 -0.11388815425884502 1102 58-59 0.0371609192743918 1102 60-61 0.061390314433516835 1102 62-63 -0.1416303331429276 1102 64-65 -0.04234263221438539 1102 66-67 -0.12474842414702891 1102 68-69 -0.14738009617817482 1102 70-71 -0.104926521380591 1102 72-73 -0.05892521855684407 1102 74-75 -0.02607369565575013 1102 76-77 -0.030735137633978837 1102 78-79 0.1075452555064551 1102 80-81 0.09125024515341806 1102 82-83 -0.08229676429402488 1102 84-85 0.06896203629928621 1102 86-87 0.02462973754751019 1102 88-89 0.11742426160053654 1102 90-91 -0.04106868557684251 1102 92-93 -0.023291354507708917 1102 94-95 -0.07445272808521253 1102 96-97 -0.2312551962439855 1102 98-99 -0.4166751903781538 1102 100-101 -0.5054119530562211 1103 1 0.3489515521857811 1103 2 0.41590588273388107 1103 3 0.46006083674643605 1103 4 0.33705543828644835 1103 5 0.3709481885403463 1103 6 0.36726000762661215 1103 7 0.36350284749052264 1103 8 0.33014420715876724 1103 9 0.5100598578981206 1103 10-11 0.462195810799102 1103 12-13 0.47789814297657784 1103 14-15 0.6851760879488396 1103 16-17 0.6764605541708164 1103 18-19 0.6947836689588911 1103 20-21 0.6430897746914326 1103 22-23 0.5510257921046886 1103 24-25 0.653648369572629 1103 26-27 0.6668400492786901 1103 28-29 0.6163499865679825 1103 30-31 0.5964315563152525 1103 32-33 0.6461273274563268 1103 34-35 0.7201413858359658 1103 36-37 0.5982588919176379 1103 38-39 0.570835969970453 1103 40-41 0.6362641335664421 1103 42-43 0.5027268893926831 1103 44-45 0.4120657286439595 1103 46-47 0.5130352913576459 1103 48-49 0.5252566250957074 1103 50-51 0.6773070226429141 1103 52-53 0.7990305317808506 1103 54-55 0.8005197336960563 1103 56-57 0.7599268480421202 1103 58-59 0.8407297181699249 1103 60-61 0.9278813406645341 1103 62-63 0.9380314247954118 1103 64-65 0.7920752232849253 1103 66-67 0.8751388284990753 1103 68-69 0.7234781642912225 1103 70-71 0.9600297602576973 1103 72-73 0.9013531983782599 1103 74-75 0.6606865436447542 1103 76-77 0.690323306912731 1103 78-79 0.6356860746504935 1103 80-81 0.8548282479145755 1103 82-83 0.9828965168840682 1103 84-85 0.8768474481722741 1103 86-87 0.7857894246160768 1103 88-89 0.9280777360598087 1103 90-91 0.9817615031024012 1103 92-93 1.0314486362884239 1103 94-95 0.7137134016892901 1103 96-97 0.8018188948742839 1103 98-99 0.6092958174451297 1103 100-101 0.45837543764787725 1104 1 0.3247167129860742 1104 2 0.3029987032555539 1104 3 0.3257187973533888 1104 4 0.30670621768882 1104 5 0.37100429170111227 1104 6 0.37839432722473987 1104 7 0.1990523995283624 1104 8 0.31010415813323533 1104 9 0.40783472022959444 1104 10-11 0.44863222433981065 1104 12-13 0.39012172701911396 1104 14-15 0.5481350549601771 1104 16-17 0.4743717903392337 1104 18-19 0.44535419494759765 1104 20-21 0.38744493359285315 1104 22-23 0.40935236428787647 1104 24-25 0.5527308671127216 1104 26-27 0.5967326764602419 1104 28-29 0.5958140719138427 1104 30-31 0.46384986148192553 1104 32-33 0.5272576766769248 1104 34-35 0.4998436938336823 1104 36-37 0.7143320159142519 1104 38-39 0.7309932314457015 1104 40-41 0.5975633101446718 1104 42-43 0.736938596827649 1104 44-45 0.6059483263066099 1104 46-47 0.4793410276900474 1104 48-49 0.4677452749947193 1104 50-51 0.3822186385865649 1104 52-53 0.427595671866122 1104 54-55 0.45514910758478777 1104 56-57 0.39873987778543096 1104 58-59 0.5238094364457524 1104 60-61 0.588606970314622 1104 62-63 0.44724744785812476 1104 64-65 0.48809362512165677 1104 66-67 0.46451763704057214 1104 68-69 0.44974350634787896 1104 70-71 0.4868928167578943 1104 72-73 0.5143428063466402 1104 74-75 0.49207237802822235 1104 76-77 0.4158442031822993 1104 78-79 0.44191697799769614 1104 80-81 0.4920397481994101 1104 82-83 0.5437032803358832 1104 84-85 0.6368454629835085 1104 86-87 0.6155430885840403 1104 88-89 0.7158532888917222 1104 90-91 0.7697821835905891 1104 92-93 0.7992980726674368 1104 94-95 0.7987929818650237 1104 96-97 0.7465641865244095 1104 98-99 0.7357752146382452 1104 100-101 0.6345643930933349 1105 1 0.14477005906815066 1105 2 0.33129704067246735 1105 3 0.28070793539079375 1105 4 0.31919600172052043 1105 5 0.26285685181645846 1105 6 0.2626792851951336 1105 7 0.30005055539352554 1105 8 0.2148867042513345 1105 9 0.16979150636803553 1105 10-11 0.25872294971355103 1105 12-13 0.3177863587687 1105 14-15 0.4675987155720307 1105 16-17 0.3795259409893106 1105 18-19 0.37399770794449694 1105 20-21 0.3477164952829881 1105 22-23 0.31127991024185064 1105 24-25 0.2232540004417345 1105 26-27 0.34325167205906126 1105 28-29 0.21056159546211717 1105 30-31 0.21885305568169855 1105 32-33 0.3533013046481841 1105 34-35 0.3708005481487149 1105 36-37 0.32275594977117095 1105 38-39 0.5000378584986862 1105 40-41 0.4056293589574196 1105 42-43 0.21034088671957818 1105 44-45 0.3196280865269969 1105 46-47 0.43684975387348146 1105 48-49 0.4609646496049109 1105 50-51 0.48338008160734347 1105 52-53 0.558978284814124 1105 54-55 0.5555660790120598 1105 56-57 0.39415815803500465 1105 58-59 0.44509997732182427 1105 60-61 0.4294752203055836 1105 62-63 0.3279417717777591 1105 64-65 0.3345384681513579 1105 66-67 0.17698873563486472 1105 68-69 0.2242536238388766 1105 70-71 0.04715461488615347 1105 72-73 0.1443471578651696 1105 74-75 0.2352322033278007 1105 76-77 0.1408501160290463 1105 78-79 0.21232682034142059 1105 80-81 0.009737466640167725 1105 82-83 0.12813831098588935 1105 84-85 0.24019949899589932 1105 86-87 0.27255757653151136 1105 88-89 0.2812472696558217 1105 90-91 0.20303435030172423 1105 92-93 0.31039276154227835 1105 94-95 0.3649158630248017 1105 96-97 0.4151800585409475 1105 98-99 0.19863075348165182 1105 100-101 0.18666604187276192 1106 1 0.17892985158777464 1106 2 0.25310501470995916 1106 3 0.308837486240364 1106 4 0.2615622052286568 1106 5 0.24802920962802943 1106 6 0.24318559611038637 1106 7 0.2815648681309355 1106 8 0.2640203315313556 1106 9 0.3280332539588855 1106 10-11 0.21814401097469727 1106 12-13 0.34079674651825087 1106 14-15 0.4015119999537333 1106 16-17 0.4601022789542242 1106 18-19 0.45676289602003095 1106 20-21 0.4807287164973886 1106 22-23 0.4817578145672954 1106 24-25 0.4584233294016684 1106 26-27 0.46907057257784146 1106 28-29 0.16098737123179063 1106 30-31 0.26708812364508816 1106 32-33 0.3803695458869285 1106 34-35 0.29387579370781935 1106 36-37 0.15178979456312192 1106 38-39 0.2130287242898632 1106 40-41 0.1319621142512517 1106 42-43 0.2594640139806188 1106 44-45 0.18461157322791877 1106 46-47 0.16497882010679632 1106 48-49 0.11159146035338097 1106 50-51 0.09376758884629766 1106 52-53 -0.12631030013062627 1106 54-55 -0.09885285397601251 1106 56-57 -0.1783606269028013 1106 58-59 -0.11071933512077692 1106 60-61 0.14449016667874304 1106 62-63 -0.04140891512540179 1106 64-65 -0.20095159600375467 1106 66-67 -0.09313232958398032 1106 68-69 0.0037302059696244783 1106 70-71 -0.007577541745050098 1106 72-73 -0.12225034329185291 1106 74-75 -0.00843648715021672 1106 76-77 -0.12391957413154842 1106 78-79 0.11443191260832108 1106 80-81 0.27182884426688503 1106 82-83 0.17936023050243932 1106 84-85 0.32898644795692533 1106 86-87 0.21738118832804076 1106 88-89 0.37171664473507704 1106 90-91 0.38253096323790814 1106 92-93 0.35874252202069457 1106 94-95 0.20817602199508656 1106 96-97 0.1133873014540363 1106 98-99 0.19133525952441488 1106 100-101 0.3786108311575731 1107 1 0.011007892072314007 1107 2 0.11159792653913314 1107 3 -0.025349507626174272 1107 4 0.1503256586321413 1107 5 0.11464788507023371 1107 6 0.11104216886160145 1107 7 0.07958346498622859 1107 8 0.08917904025020107 1107 9 0.1402650300844286 1107 10-11 0.1146433632466568 1107 12-13 0.1321170372898095 1107 14-15 0.21497553378601708 1107 16-17 0.22312898008452464 1107 18-19 0.1934295985010408 1107 20-21 0.23540901205783626 1107 22-23 0.3238107901255347 1107 24-25 0.21615001886672758 1107 26-27 0.12320797380699133 1107 28-29 0.13299961052893394 1107 30-31 0.2766977523833347 1107 32-33 0.2442768194737468 1107 34-35 0.04031060341083759 1107 36-37 0.13475777039764836 1107 38-39 0.12427309887927862 1107 40-41 0.0581172804195873 1107 42-43 0.029333301848154747 1107 44-45 -0.018871730123684927 1107 46-47 -0.0942183041034994 1107 48-49 0.02250890112156867 1107 50-51 0.03372112740607491 1107 52-53 0.008749821924673995 1107 54-55 0.024596128998872757 1107 56-57 -0.03562830435265596 1107 58-59 0.11885842908166211 1107 60-61 -0.04006627775478222 1107 62-63 -0.06120989991761405 1107 64-65 -0.021064702826699033 1107 66-67 -0.15360012123043987 1107 68-69 -0.0064070448392712365 1107 70-71 0.053292393850519204 1107 72-73 0.09111273129817477 1107 74-75 -0.0277486061111496 1107 76-77 0.13071122088554787 1107 78-79 -0.015427198868440684 1107 80-81 -0.06195331957453831 1107 82-83 0.04817227885794395 1107 84-85 -0.1100423130099415 1107 86-87 -0.04047558396025153 1107 88-89 0.06095146610335078 1107 90-91 0.03548725847910461 1107 92-93 0.025032882231158737 1107 94-95 -0.27239789267672165 1107 96-97 -0.044892412686081684 1107 98-99 0.05063207552667137 1107 100-101 0.20830286870738135 1108 1 -0.10928617734236212 1108 2 0.050655100520927476 1108 3 0.1326154336302281 1108 4 -0.019677914009619712 1108 5 0.07929337358506672 1108 6 0.08498384694900807 1108 7 0.036394032977703716 1108 8 0.11142166362348149 1108 9 0.04254302846795355 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pass #Quality Count 2 4411.0 3 772.0 4 695.0 5 691.0 6 855.0 7 959.0 8 1306.0 9 1523.0 10 2042.0 11 3067.0 12 4921.0 13 8442.0 14 7864.0 15 9236.0 16 13045.0 17 19994.0 18 17785.0 19 8291.0 20 7392.0 21 7283.0 22 7353.0 23 7966.0 24 8797.0 25 9601.0 26 10905.0 27 12743.0 28 14474.0 29 17384.0 30 20761.0 31 25056.0 32 31122.0 33 39796.0 34 54129.0 35 82908.0 36 127467.0 37 236630.0 38 316824.0 39 68905.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.233479367505 22.202072924470727 15.78318473570792 19.781262972316355 2 14.042473138685407 23.689113390969712 40.921362480465625 21.347050989879254 3 18.8616307160207 28.737277563628684 32.413500633646635 19.987591086703983 4 10.878392148870459 19.385405209607963 42.14346595948853 27.59273668203305 5 10.404433943501221 40.51948309171169 36.10699628551598 12.969086679271099 6 25.064959490024698 41.290973971660335 18.90803715930294 14.736029379012022 7 23.38509893210251 36.880204917525646 23.668221697004192 16.06647445336765 8 23.384520702092484 34.283107343327714 21.873484096001476 20.458887858578326 9 24.881071106568704 18.846569147861178 24.61793036091527 31.654429384654854 10-11 22.310443614610683 28.52149360178373 29.191670976687938 19.976391806917647 12-13 25.00880591757661 27.692362897843932 28.86687019953755 18.4319609850419 14-15 21.814849858978558 28.535208339692154 29.993474819485126 19.656466981844158 16-17 17.76086059074883 31.551419876581242 30.79872465360389 19.888994879066033 18-19 17.381584163071558 30.821796606855667 33.91878018065807 17.87783904941471 20-21 19.377251842519737 28.610517770115912 33.35197922880246 18.66025115856189 22-23 18.692730428354015 28.797753091747243 33.47267871943062 19.036837760468128 24-25 19.148938803853554 29.34912175234452 33.521783207276016 17.98015623652591 26-27 18.744257109837275 28.428703991378292 35.18756222538825 17.63947667339619 28-29 17.488254726887074 30.115429000045797 34.4170437352836 17.979272537783533 30-31 18.367773794446347 28.98188523880049 33.91115474898824 18.739186217764924 32-33 18.327008777100747 29.447158569873388 34.04064684142765 18.185185811598213 34-35 19.193406688812498 28.873932070042564 33.80275052039945 18.12991072074549 36-37 18.970844060091654 28.254291773071554 34.37799571081502 18.396868456021775 38-39 18.09141837077712 29.931460233190865 33.8111438805199 18.16597751551211 40-41 18.165679934571052 29.85957279135914 33.940272907581615 18.034474366488194 42-43 17.79329726900933 30.075509850893095 32.51622770372713 19.614965176370443 44-45 18.643403197443437 29.132211814168834 32.67506294726094 19.549322041126786 46-47 19.532559234464504 30.081778530868508 32.040049077884845 18.345613156782147 48-49 18.85438473341243 29.944899645295347 32.15943280386468 19.041282817427533 50-51 17.695662742100385 30.338527486387967 32.750866577859085 19.214943193652566 52-53 18.507178380795814 31.419733404005978 31.000335419004593 19.072752796193615 54-55 18.327351742390242 30.062131809200775 31.468109314885634 20.142407133523353 56-57 19.254945840096337 30.17315356061989 30.835400007534 19.736500591749778 58-59 19.888144732256748 29.67980570807042 31.003146317417986 19.42890324225485 60-61 19.068046235696144 30.357441315913587 31.93767117053757 18.636841277852703 62-63 18.350119562553406 30.176296806586873 32.050506070863165 19.423077559996553 64-65 18.25172213014889 30.979541499671598 31.408034481530535 19.360701888648972 66-67 18.710805520835663 30.06466597254492 30.410028816820606 20.81449968979881 68-69 18.182469612904963 29.936357442120066 31.216705705463156 20.66446723951182 70-71 19.333014419462856 29.925676501224917 31.06643555765404 19.67487352165819 72-73 19.109231461165542 28.8929916500803 32.135512048616896 19.862264840137264 74-75 18.144859348596796 31.130553990921438 30.71423246413306 20.010354196348697 76-77 18.483101077181587 29.70506077417613 31.01516103893738 20.796677109704902 78-79 18.60892108108824 29.75393832809217 31.231691100612213 20.405449490207378 80-81 19.167336313312656 29.460809214669048 30.816828147763587 20.555026324254705 82-83 18.753056244842426 29.311836140144433 31.67079134641223 20.26431626860091 84-85 18.492006937851247 29.55700188434955 31.13896943345712 20.812021744342086 86-87 18.31256456812079 29.80835043073018 31.1499595252341 20.729125475914927 88-89 18.676274675530927 29.349165207258103 30.57374323492178 21.40081688228919 90-91 18.896020549786304 28.306112956728068 31.3438498295042 21.45401666398142 92-93 19.606188175705757 29.07320244926189 31.24544180103992 20.075167573992434 94-95 19.205293076134563 28.259849449673325 31.431015023970016 21.1038424502221 96-97 18.66219757864115 28.1368243355179 31.910381890833428 21.29059619500752 98-99 19.133775994029754 28.65883582694073 30.544333505477628 21.663054673551887 100-101 19.077884906838573 27.443448057680747 31.681883005348354 21.796784030132326 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4035.0 1 2460.0 2 1035.5 3 1165.5 4 1019.0 5 988.0 6 1549.5 7 2971.5 8 4573.5 9 4915.0 10 3976.5 11 3212.5 12 3145.0 13 3213.0 14 3325.0 15 3479.5 16 3637.5 17 3775.5 18 4015.5 19 4342.0 20 4696.5 21 5265.0 22 6031.0 23 6864.0 24 7712.0 25 9486.5 26 13314.5 27 17118.5 28 18086.0 29 19322.5 30 23198.0 31 27025.5 32 31667.5 33 37970.5 34 44849.0 35 49299.0 36 53569.0 37 58524.0 38 56233.5 39 51311.0 40 47734.5 41 44821.5 42 41766.0 43 39845.5 44 39522.5 45 38264.0 46 37750.5 47 44517.5 48 64252.0 49 60811.0 50 35668.0 51 22990.5 52 17643.0 53 14601.0 54 12089.5 55 10241.5 56 8627.0 57 6994.5 58 5694.0 59 4707.5 60 3738.5 61 2892.0 62 2100.5 63 1452.0 64 1066.0 65 765.5 66 514.0 67 356.0 68 284.0 69 238.0 70 195.0 71 151.0 72 117.0 73 113.5 74 115.5 75 98.0 76 68.0 77 53.5 78 46.0 79 36.0 80 27.5 81 22.5 82 18.5 83 11.5 84 6.5 85 3.5 86 2.0 87 2.5 88 2.5 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33797398564537706 2 0.11875652604608346 3 0.11331729584549949 4 0.11982788957044092 5 0.13713453111775356 6 0.09172520020075704 7 0.09988404550163302 8 0.10375743670507917 9 0.09296138888270794 10-11 0.09197243793714723 12-13 0.09510411593142284 14-15 0.09724684298013775 16-17 0.08492616245002707 18-19 0.10116144047298227 20-21 0.11076250590280096 22-23 0.11789119396871783 24-25 0.13107720657619415 26-27 0.13041790594582034 28-29 0.1266269273211709 30-31 0.11636656126097839 32-33 0.10981476124663861 34-35 0.3296091088975093 36-37 0.49047846270872014 38-39 0.46488935699233647 40-41 0.44329726134759406 42-43 0.4738723280811797 44-45 0.4819899670926573 46-47 0.48940709918436265 48-49 0.4997910841127503 50-51 0.48850056081759874 52-53 0.4913437947860858 54-55 0.47906432054537346 56-57 0.4572661934536392 58-59 0.43909421982896096 60-61 0.3969813920638335 62-63 0.46736173435623823 64-65 0.4974423256170436 66-67 0.504859457708749 68-69 0.517097725660063 70-71 0.5096805935683575 72-73 0.5018926048720668 74-75 0.5082383734394148 76-77 0.4865638652158756 78-79 0.466537608568271 80-81 0.46814465385480714 82-83 0.48116584130469003 84-85 0.4803417155167227 86-87 0.48330856835340485 88-89 0.4954232174365238 90-91 0.5103398941987313 92-93 0.5030875872646193 94-95 0.5084444048864066 96-97 0.5069197721786671 98-99 0.5016041608462782 100-101 0.48895383000098075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1213407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.46324795003212 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14155803703659 26.29300456069126 2 12.899817964824 9.149388859936645 3 4.906628737217128 5.22014974520052 4 2.37952597794398 3.3754287903748006 5 1.313586007952886 2.329201315186303 6 0.802145698612485 1.7068015081167776 7 0.5277870129660163 1.3101929193934456 8 0.40347290129356383 1.1446767631753993 9 0.2861024808120759 0.913151089454216 >10 1.8093297377193511 12.75899759918944 >50 0.28975222317234317 7.267199194909761 >100 0.21637998212264356 14.720758499872383 >500 0.015090878555847662 3.4738398149956184 >1k 0.007429355596725002 5.616996109656596 >5k 9.286694495906253E-4 2.532843530259533 >10k+ 4.6433472479531264E-4 2.1873696995873293 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13517 1.1139708275953575 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13050 1.075484153297286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9457 0.7793757576806463 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8303 0.6842716417492235 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6565 0.5410385798005122 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6438 0.5305721822933278 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4830 0.3980527555881909 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3865 0.3185246170493495 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3827 0.3153929390550739 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3483 0.2870430119489998 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3473 0.2862188861610325 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3343 0.27550525091745803 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3322 0.27377458676272676 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3049 0.25127595275122033 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2997 0.24699049865379052 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2389 0.19688365074538058 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2205 0.1817197362467828 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2107 0.17364330352470358 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2034 0.16762718527254253 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.16119900412639782 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 0.16086935381121092 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1935 0.15946833997166654 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1799 0.14826022925531168 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1792 0.1476833412037346 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1709 0.1408430971636063 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1571 0.12947016128965796 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1427 0.1176027499429293 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1390 0.1145534845274504 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1367 0.11265799521512566 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1265 0.10425191217785952 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.102603660601925 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1224 0.10087299644719373 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.24125787967269E-5 0.0 9 0.0 0.0 0.0 8.24125787967269E-5 0.0 10-11 0.0 0.0 0.0 8.24125787967269E-5 0.0 12-13 0.0 0.0 0.0 8.24125787967269E-5 0.0 14-15 0.0 0.0 0.0 1.2361886819509035E-4 0.0 16-17 0.0 0.0 0.0 3.708566045852711E-4 0.0 18-19 0.0 0.0 0.0 5.768880515770883E-4 0.0 20-21 0.0 0.0 0.0 9.477446561623594E-4 0.0 22-23 0.0 0.0 0.0 0.0020603144699181727 0.0 24-25 0.0 0.0 0.0 0.0041618352292347085 0.0 26-27 0.0 0.0 0.0 0.0077055761174939655 0.0 28-29 0.0 0.0 0.0 0.019655400043019366 0.0 30-31 0.0 0.0 0.0 0.05113700514336904 0.0 32-33 0.0 0.0 0.0 0.10528206941281862 0.0 34-35 0.0 0.0 0.0 0.18435693876827808 0.0 36-37 0.0 0.0 0.0 0.2951606509604774 0.0 38-39 0.0 0.0 0.0 0.4506319808605027 0.0 40-41 0.0 0.0 0.0 0.6152511070069646 0.0 42-43 0.0 0.0 0.0 0.786133589141978 0.0 44-45 0.0 0.0 0.0 0.9924534801595837 0.0 46-47 0.0 0.0 0.0 1.2373836643434561 0.0 48-49 0.0 0.0 0.0 1.4724655453611195 0.0 50-51 0.0 0.0 0.0 1.7431908667083675 0.0 52-53 0.0 0.0 0.0 1.9930658056200432 0.0 54-55 0.0 0.0 0.0 2.2782133282567187 0.0 56-57 0.0 0.0 0.0 2.627230599460857 0.0 58-59 0.0 0.0 0.0 2.98947508956187 0.0 60-61 0.0 0.0 0.0 3.393131900508238 0.0 62-63 0.0 0.0 0.0 3.794975634721079 0.0 64-65 0.0 0.0 0.0 4.213631535008451 0.0 66-67 0.0 0.0 0.0 4.680169143576722 0.0 68-69 0.0 0.0 0.0 5.219477059222504 0.0 70-71 0.0 0.0 0.0 5.812600388822547 0.0 72-73 0.0 0.0 0.0 6.375601920872386 0.0 74-75 0.0 0.0 0.0 6.989658045486799 0.0 76-77 0.0 0.0 0.0 7.6279022619780505 0.0 78-79 8.24125787967269E-5 0.0 0.0 8.40843179576185 0.0 80-81 8.24125787967269E-5 0.0 0.0 9.240057128399622 0.0 82-83 8.24125787967269E-5 0.0 0.0 10.030970647111811 0.0 84-85 8.24125787967269E-5 0.0 0.0 10.834864146984483 0.0 86-87 8.24125787967269E-5 0.0 0.0 11.683342851986184 0.0 88-89 8.24125787967269E-5 0.0 0.0 12.581763579738702 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 75 9.422365E-10 31.707682 94-95 CATATAG 345 0.0 26.08631 3 CATTCCG 360 0.0 24.991129 9 GACATAT 335 0.0 24.089788 1 GAGTACT 8165 0.0 23.864286 12-13 AGTACTT 8425 0.0 22.172358 12-13 GTACTTT 8800 0.0 22.035551 14-15 GTACATG 7130 0.0 21.704859 1 ACACCGT 110 0.0029294684 21.52435 6 GCGCCGT 90 5.114978E-6 21.138454 94-95 CATGGGT 695 0.0 21.127836 4 CGTATAG 205 6.869959E-7 20.840952 1 TACATGG 7345 0.0 20.63651 2 CGCCGTA 150 1.6370905E-10 20.609993 94-95 ACATGGG 7240 0.0 20.346977 3 GATTGGC 425 0.0 20.0548 9 GTATAGG 450 0.0 20.043335 1 ATGGGTA 430 0.0 19.828148 5 GGCATTC 485 0.0 19.527243 7 CTTTGCG 170 8.127201E-5 19.50416 2 >>END_MODULE