##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1213407 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.451430558749045 34.0 31.0 34.0 31.0 34.0 2 32.645203134644845 34.0 31.0 34.0 31.0 34.0 3 32.71899947832838 34.0 31.0 34.0 31.0 34.0 4 36.1939852003491 37.0 35.0 37.0 35.0 37.0 5 36.16910813931352 37.0 35.0 37.0 35.0 37.0 6 36.234735748186715 37.0 37.0 37.0 35.0 37.0 7 36.19664712664424 37.0 37.0 37.0 35.0 37.0 8 36.197762168835354 37.0 37.0 37.0 35.0 37.0 9 37.91300445769639 39.0 38.0 39.0 35.0 39.0 10-11 37.86629342009729 39.0 38.0 39.0 35.0 39.0 12-13 37.82522805620867 39.0 38.0 39.0 35.0 39.0 14-15 39.10025078147728 40.0 38.0 41.0 36.0 41.0 16-17 39.074416910401865 40.0 38.0 41.0 36.0 41.0 18-19 39.189951928742786 40.5 38.5 41.0 36.0 41.0 20-21 39.27730266926102 41.0 39.0 41.0 36.0 41.0 22-23 39.218163402716485 41.0 39.0 41.0 35.5 41.0 24-25 39.15360509705317 41.0 39.0 41.0 35.0 41.0 26-27 38.98834644929525 40.5 38.5 41.0 35.0 41.0 28-29 38.84026423121014 40.0 38.0 41.0 35.0 41.0 30-31 38.59474026439604 40.0 38.0 41.0 35.0 41.0 32-33 38.33275520909308 40.0 38.0 41.0 34.0 41.0 34-35 38.18168306264921 40.0 38.0 41.0 34.0 41.0 36-37 38.02222873281595 40.0 37.5 41.0 34.0 41.0 38-39 37.846529647513165 40.0 37.0 41.0 33.0 41.0 40-41 37.68479784606484 40.0 37.0 41.0 33.0 41.0 42-43 37.43041287877851 40.0 37.0 41.0 33.0 41.0 44-45 37.058006917711865 40.0 37.0 41.0 32.5 41.0 46-47 36.564868176959585 40.0 37.0 41.0 30.5 41.0 48-49 36.088507401061634 40.0 36.0 41.0 29.5 41.0 50-51 35.793470781032255 40.0 36.0 41.0 27.5 41.0 52-53 35.604580326304365 40.0 36.0 41.0 27.0 41.0 54-55 35.4061955304362 40.0 35.0 41.0 26.0 41.0 56-57 35.13688276068953 39.0 35.0 41.0 25.0 41.0 58-59 34.876765174422104 39.0 35.0 40.5 23.5 41.0 60-61 34.51449390023298 39.0 35.0 40.0 22.0 41.0 62-63 34.17194148377255 38.0 34.5 40.0 21.5 41.0 64-65 33.79351816826506 38.0 34.0 40.0 19.5 41.0 66-67 33.40779309827617 37.0 34.0 40.0 16.0 41.0 68-69 32.924931206099856 37.0 33.5 39.0 10.5 40.5 70-71 32.49531731727277 36.0 33.0 39.0 7.5 40.0 72-73 32.019319568784425 36.0 32.5 38.5 3.5 40.0 74-75 31.525820685062804 35.0 32.0 37.5 2.0 39.5 76-77 29.52711827111596 33.5 29.5 35.5 2.0 38.5 78-79 30.420243166554997 34.5 31.0 36.0 2.0 39.0 80-81 30.3850595884151 35.0 31.0 36.0 2.0 38.5 82-83 30.056063217040943 35.0 31.0 36.0 2.0 37.0 84-85 29.65200134826979 34.5 31.0 35.0 2.0 37.0 86-87 29.157073842494725 34.0 30.0 35.0 2.0 36.0 88-89 28.60573657478488 34.0 29.0 35.0 2.0 36.0 90-91 28.160177088149318 34.0 29.0 35.0 2.0 36.0 92-93 27.91051518575383 34.0 29.0 35.0 2.0 35.0 94-95 27.602630444690035 34.0 28.5 35.0 2.0 35.0 96-97 27.095798029844893 34.0 27.0 35.0 2.0 35.0 98-99 26.451969537014374 33.5 25.5 35.0 2.0 35.0 100-101 23.120975072667292 29.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1701582358316287 1101 2 0.12700487722800347 1101 3 0.22631290273994864 1101 4 0.15423904676735134 1101 5 0.0648570815660321 1101 6 0.09566594259479189 1101 7 0.1212890244871545 1101 8 0.20604243709314574 1101 9 0.2952369617259478 1101 10-11 0.16169352320910235 1101 12-13 0.20274154166418157 1101 14-15 0.32595801903621435 1101 16-17 0.2881901119820611 1101 18-19 0.27701657537453883 1101 20-21 0.3462688271851775 1101 22-23 0.141238511226625 1101 24-25 0.17046194913287138 1101 26-27 0.23403634799544193 1101 28-29 0.11534936027289433 1101 30-31 0.14429634342427988 1101 32-33 0.1469211287601695 1101 34-35 0.1210020532488798 1101 36-37 0.11945467595248971 1101 38-39 0.15522306514996842 1101 40-41 0.15186710746602472 1101 42-43 0.17745648032633454 1101 44-45 0.31916211300386976 1101 46-47 0.2697811973511506 1101 48-49 0.39191258726192046 1101 50-51 0.2850118185833139 1101 52-53 0.19848198812760387 1101 54-55 0.4316981389795629 1101 56-57 0.45446554287681096 1101 58-59 0.3899096224247458 1101 60-61 0.40205211112550643 1101 62-63 0.39245901957105644 1101 64-65 0.5008585294553995 1101 66-67 0.43534383206274896 1101 68-69 0.34664115149093533 1101 70-71 0.3241042642357215 1101 72-73 0.2274220625525345 1101 74-75 0.335690726669565 1101 76-77 0.33513475681290217 1101 78-79 0.49664636791434447 1101 80-81 0.3942570056702337 1101 82-83 0.5279218318750161 1101 84-85 0.2666534776023788 1101 86-87 0.40710875168212723 1101 88-89 0.5669492845177295 1101 90-91 0.5118911981914955 1101 92-93 0.5785399844665875 1101 94-95 0.5920471746172069 1101 96-97 0.49409147177498625 1101 98-99 0.5665721565948836 1101 100-101 0.06901301678803762 1102 1 0.07701050474724269 1102 2 0.11183500804404645 1102 3 0.14750989349178667 1102 4 0.10006970468100462 1102 5 0.029975678528039396 1102 6 -0.020794836551310425 1102 7 0.024400817098204186 1102 8 0.07249315346671636 1102 9 0.22637693010008775 1102 10-11 0.09694101949545342 1102 12-13 0.1921789847615969 1102 14-15 0.2569983180443174 1102 16-17 0.19721402304631397 1102 18-19 0.22387267772731434 1102 20-21 0.143304621904619 1102 22-23 0.06342644548317367 1102 24-25 0.12902920199596934 1102 26-27 0.0923156138922252 1102 28-29 0.09013444820235605 1102 30-31 0.2292472275107258 1102 32-33 0.2404052439549602 1102 34-35 0.22638851163212337 1102 36-37 0.33515595536214704 1102 38-39 0.1850510396576155 1102 40-41 0.13697516247584218 1102 42-43 0.15834440020750407 1102 44-45 0.3216701392629133 1102 46-47 0.4280901723858932 1102 48-49 0.49066863690493534 1102 50-51 0.3899933701670051 1102 52-53 0.24924771719943095 1102 54-55 0.41724703926228557 1102 56-57 0.4314879205662052 1102 58-59 0.49798332025405756 1102 60-61 0.48472928779904123 1102 62-63 0.498029003758127 1102 64-65 0.4457437659305512 1102 66-67 0.4228875572052999 1102 68-69 0.47321453785348666 1102 70-71 0.5128181460700176 1102 72-73 0.4004308315400351 1102 74-75 0.46348075014936896 1102 76-77 0.43840707460484296 1102 78-79 0.4060790192153192 1102 80-81 0.5739977694825882 1102 82-83 0.6382759456322624 1102 84-85 0.5843754575726692 1102 86-87 0.6316293324478579 1102 88-89 0.5691544692414823 1102 90-91 0.7586342141481808 1102 92-93 0.7601088186225127 1102 94-95 0.639958430547054 1102 96-97 0.7066665831842585 1102 98-99 0.66819945066743 1102 100-101 0.27334527995349944 1103 1 0.21274451303072084 1103 2 0.17484007017013425 1103 3 0.18231431134728382 1103 4 0.07391692051165677 1103 5 0.2237998846946283 1103 6 0.1548793466056182 1103 7 0.19028668916447344 1103 8 0.1632800839775257 1103 9 0.3145867782363041 1103 10-11 0.37610116675730865 1103 12-13 0.2277795369935376 1103 14-15 0.3862389991303701 1103 16-17 0.35941374693035044 1103 18-19 0.2441533956288282 1103 20-21 0.338976504095136 1103 22-23 0.3166685071490747 1103 24-25 0.34186629357443366 1103 26-27 0.45376982466075333 1103 28-29 0.3237722760900752 1103 30-31 0.4050962841145065 1103 32-33 0.423267646163886 1103 34-35 0.46923480707621223 1103 36-37 0.5119668159235786 1103 38-39 0.45045140781223836 1103 40-41 0.48857893606075464 1103 42-43 0.42034394001422015 1103 44-45 0.5473926427143638 1103 46-47 0.4830832694866771 1103 48-49 0.628563252643545 1103 50-51 0.7242915754132042 1103 52-53 0.4722642841575535 1103 54-55 0.5469110981670227 1103 56-57 0.6659633002256626 1103 58-59 0.756091925868418 1103 60-61 0.6373961078634665 1103 62-63 0.7766622297130326 1103 64-65 0.879673816551815 1103 66-67 0.8008097845407818 1103 68-69 0.7758169308585465 1103 70-71 0.8369253711045346 1103 72-73 0.8406333165837481 1103 74-75 0.7351328440287936 1103 76-77 0.7635543364548205 1103 78-79 0.876373542915271 1103 80-81 0.8880359655249244 1103 82-83 1.0346933409382935 1103 84-85 0.8977762859205747 1103 86-87 0.9658838193323511 1103 88-89 1.0321020072074276 1103 90-91 1.1302586964307366 1103 92-93 1.0470761448995525 1103 94-95 0.9153058764743456 1103 96-97 0.7925225426872444 1103 98-99 0.7255418344686291 1103 100-101 0.2743369964744353 1104 1 0.08785110903617976 1104 2 0.10463084853675753 1104 3 0.10776874694948901 1104 4 0.09107499332492353 1104 5 0.16477763331793938 1104 6 0.1382848947766533 1104 7 0.15445057944395302 1104 8 0.08807077603159286 1104 9 0.20013633027414812 1104 10-11 0.19739490564456474 1104 12-13 0.20914638261490381 1104 14-15 0.2439717409349953 1104 16-17 0.1701941850528783 1104 18-19 0.10345271030714542 1104 20-21 0.036914065592831946 1104 22-23 0.12198579210134852 1104 24-25 0.1119542460680023 1104 26-27 0.2830781158447877 1104 28-29 0.2335173951150793 1104 30-31 0.269521701435707 1104 32-33 0.14654096350061963 1104 34-35 0.2600144683857408 1104 36-37 0.3046639106429794 1104 38-39 0.20273653270650271 1104 40-41 0.31918925213732763 1104 42-43 0.2898285677481667 1104 44-45 0.24561676189473758 1104 46-47 0.17665643663957553 1104 48-49 0.3526198564171281 1104 50-51 0.31376058057469436 1104 52-53 0.4327801742989337 1104 54-55 0.43798120122421125 1104 56-57 0.43957798371751977 1104 58-59 0.3205285638217745 1104 60-61 0.488616998954285 1104 62-63 0.4862627752083739 1104 64-65 0.5386382385166328 1104 66-67 0.5866212951831145 1104 68-69 0.30296438722755425 1104 70-71 0.19783053086907643 1104 72-73 0.09731477146817724 1104 74-75 0.2878352901266048 1104 76-77 0.33082936278014685 1104 78-79 0.36014591944516283 1104 80-81 0.30708816735820577 1104 82-83 0.5448441289719206 1104 84-85 0.4169445781403631 1104 86-87 0.5610127702895547 1104 88-89 0.4895115084934858 1104 90-91 0.4803096208382023 1104 92-93 0.4694828841838472 1104 94-95 0.422092632675902 1104 96-97 0.37616070319407413 1104 98-99 0.10965126152589022 1104 100-101 -0.3139378744882926 1105 1 0.11484567426729342 1105 2 0.1475215025354828 1105 3 0.13821422629408175 1105 4 0.05775768008881954 1105 5 0.12347355812504901 1105 6 0.06955362755891059 1105 7 0.085618309861907 1105 8 0.10993610452916158 1105 9 0.025145525697730875 1105 10-11 0.1399270854186696 1105 12-13 0.08800414687706848 1105 14-15 0.09101586465951783 1105 16-17 0.09351487345132625 1105 18-19 0.1420314050367537 1105 20-21 0.13354134414304752 1105 22-23 0.14153311633829446 1105 24-25 0.022041520527615432 1105 26-27 0.011050552299565197 1105 28-29 -0.05712069573822731 1105 30-31 -0.05761569797225263 1105 32-33 0.021104864750611796 1105 34-35 0.0037668408891917693 1105 36-37 0.04460554811013395 1105 38-39 0.042801393058113035 1105 40-41 -0.05535234442387349 1105 42-43 0.023138979156051676 1105 44-45 -0.17106260020799624 1105 46-47 -0.31250264292587815 1105 48-49 -0.5020259577503055 1105 50-51 -0.4369857848131389 1105 52-53 -0.44024764932737526 1105 54-55 -0.44799091707312044 1105 56-57 -0.34164332003782505 1105 58-59 -0.30551523248675494 1105 60-61 -0.33124486347881543 1105 62-63 -0.356662135425573 1105 64-65 -0.4757470232183678 1105 66-67 -0.42600063360853113 1105 68-69 -0.39788470598164594 1105 70-71 -0.31740062566691307 1105 72-73 -0.30831914041230846 1105 74-75 -0.3609252004418799 1105 76-77 -0.17085358911495163 1105 78-79 -0.11268149865664867 1105 80-81 -0.1971261562920894 1105 82-83 -0.20827284607460328 1105 84-85 -0.22449029511429686 1105 86-87 -0.04785010300886938 1105 88-89 -0.0693417274960133 1105 90-91 0.07246417802279126 1105 92-93 0.0947649543283049 1105 94-95 -0.07827434258104304 1105 96-97 0.14271478028076245 1105 98-99 0.18920198617576744 1105 100-101 -0.004400137059089815 1106 1 0.029352096644288395 1106 2 0.06912916851857176 1106 3 0.11386548997136714 1106 4 0.06589681018648008 1106 5 0.03385670381765493 1106 6 0.08863620051586452 1106 7 0.08359180619861206 1106 8 0.03403065962316276 1106 9 0.15710007192325293 1106 10-11 0.1586922347249029 1106 12-13 0.1723237416086434 1106 14-15 0.2789585313251095 1106 16-17 0.22318203092657285 1106 18-19 0.1944664805924745 1106 20-21 0.26159109484836307 1106 22-23 0.19421211541187233 1106 24-25 0.2651319022679246 1106 26-27 0.24809453849447038 1106 28-29 0.1997869032870483 1106 30-31 0.25511202118939025 1106 32-33 0.28336505037479043 1106 34-35 0.3213100302941925 1106 36-37 0.34262677914851736 1106 38-39 0.4196046703563425 1106 40-41 0.30131230031606293 1106 42-43 0.39401539958650034 1106 44-45 0.3300184979001486 1106 46-47 0.38852829739927586 1106 48-49 0.33813346484557627 1106 50-51 0.5717090385967367 1106 52-53 0.6579977975009328 1106 54-55 0.723081853712273 1106 56-57 0.7251541660960896 1106 58-59 0.6428056933241493 1106 60-61 0.5896899935957407 1106 62-63 0.5389305219919223 1106 64-65 0.5306329498531213 1106 66-67 0.6321468561196255 1106 68-69 0.5576990021889046 1106 70-71 0.4721337631067897 1106 72-73 0.32712262365549805 1106 74-75 0.2930038662760488 1106 76-77 0.3492886780652995 1106 78-79 0.47418111990269907 1106 80-81 0.36557874552061875 1106 82-83 0.5394819671744848 1106 84-85 0.5812738922286584 1106 86-87 0.6156456925412215 1106 88-89 0.9005123141632154 1106 90-91 0.9157921832695735 1106 92-93 0.8781229088599929 1106 94-95 0.8126910372409917 1106 96-97 0.7867201175211775 1106 98-99 0.9635149820492614 1106 100-101 0.6431142257553368 1107 1 -0.06635893069536536 1107 2 0.010847459007713667 1107 3 0.07516297212047363 1107 4 0.11890306644119875 1107 5 0.10107920914376223 1107 6 0.1308920131843223 1107 7 0.1417545609153592 1107 8 0.10815407790812515 1107 9 0.2100380251026479 1107 10-11 0.18207490999520104 1107 12-13 0.15258492028948467 1107 14-15 0.2671236804867547 1107 16-17 0.28643413220545 1107 18-19 0.28968710720027246 1107 20-21 0.30283387314118926 1107 22-23 0.14024846492714715 1107 24-25 0.21335164784280636 1107 26-27 0.2224563268378148 1107 28-29 0.11341305983462746 1107 30-31 0.18218429044779327 1107 32-33 0.24332141315161238 1107 34-35 0.17465603678989083 1107 36-37 0.12967198810988378 1107 38-39 0.26215150017271327 1107 40-41 0.46064649114717326 1107 42-43 0.5012170763118817 1107 44-45 0.3259567672690551 1107 46-47 0.08385513912647014 1107 48-49 0.23024223403950117 1107 50-51 0.13152373005102191 1107 52-53 0.22132088793113525 1107 54-55 0.0037061898677777094 1107 56-57 0.16168832991295545 1107 58-59 0.172654137241949 1107 60-61 0.33377755660340824 1107 62-63 0.26725465615451327 1107 64-65 0.23611431002061067 1107 66-67 0.11163904961532722 1107 68-69 0.20440249623656115 1107 70-71 0.1975930053570707 1107 72-73 0.07183796099594275 1107 74-75 0.2410455633849118 1107 76-77 0.17068918274060607 1107 78-79 0.42368519352880796 1107 80-81 0.09331040806351965 1107 82-83 -0.028867155169372438 1107 84-85 0.22073610911624897 1107 86-87 0.0661163698007492 1107 88-89 0.060118566120213046 1107 90-91 -0.0170348119476742 1107 92-93 0.15672616469107936 1107 94-95 0.2799516081012072 1107 96-97 0.2025799381219997 1107 98-99 0.3703515059292428 1107 100-101 0.16466883444778446 1108 1 0.01475614351016219 1108 2 0.0034532619150269284 1108 3 -6.456416233646678E-5 1108 4 -0.00777929642207198 1108 5 0.01537094755610724 1108 6 -0.023803375179404895 1108 7 -0.07225400171129337 1108 8 -0.035676965420407214 1108 9 -0.1015505119883997 1108 10-11 0.03410897308453542 1108 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2316 98-99 -1.410809254532161 2316 100-101 -0.8444839099139649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 7.0 5 22.0 6 73.0 7 183.0 8 361.0 9 754.0 10 1373.0 11 2328.0 12 3806.0 13 5649.0 14 6414.0 15 9168.0 16 12838.0 17 19986.0 18 18671.0 19 10046.0 20 7415.0 21 7018.0 22 7432.0 23 7844.0 24 8472.0 25 9505.0 26 10842.0 27 12534.0 28 15009.0 29 17913.0 30 21792.0 31 26706.0 32 32745.0 33 42446.0 34 57449.0 35 86597.0 36 133408.0 37 264530.0 38 315191.0 39 36874.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45041469600951 22.711811703449968 16.128610758030728 19.709162842509794 2 14.119499887506832 24.161390201309207 40.43062220672866 21.288487704455306 3 18.80152331410648 28.689219692980178 32.65656123625461 19.85269575665873 4 11.014688393918942 19.877914005770528 41.53289044813488 27.574507152175652 5 10.273552072799976 40.81746685160049 36.006632564341565 12.902348511257971 6 25.05210535294423 41.66878879057068 18.56780124063896 14.711304615846126 7 23.429154438700287 36.94036708210848 23.37319629769731 16.257282181493927 8 23.424539334287672 34.70055801557103 21.477459747636203 20.397442902505095 9 24.646553052685537 18.917725050209864 24.34409888850155 32.09162300860305 10-11 22.29482770414214 28.75135877739291 28.95586559167699 19.997947926787962 12-13 25.048685230924168 27.725528202820655 28.73697778239288 18.488808783862297 14-15 21.675045327179827 28.761373001483438 29.82911653205868 19.73446513927806 16-17 17.725630816878706 31.62651322290415 30.6774755292417 19.970380430975442 18-19 17.355024882428104 30.848606794178206 33.9316214310703 17.86474689232339 20-21 19.289776637187686 28.491676741604426 33.49164789720185 18.72689872400604 22-23 18.53788547453575 28.867848957522085 33.538004972775006 19.056260595167164 24-25 19.04138117458188 29.412140411833835 33.65665294766285 17.889825465921433 26-27 18.686098662099862 28.391355157516852 35.39258476636608 17.52996141401721 28-29 17.38015356759933 30.190529640920154 34.520733768636575 17.908583022843942 30-31 18.42969645297953 28.877206096571495 34.051017393285896 18.642080057163085 32-33 18.336098275351965 29.445808372623528 34.06490979531188 18.15318355671263 34-35 19.14434518290306 28.8672233335229 33.99261349072318 17.99581799285086 36-37 18.89071433752976 28.252050950625506 34.60430996392701 18.252924747917724 38-39 17.999545817793805 29.929164885863024 33.965451653705436 18.105837642637734 40-41 18.102021867539467 29.869454968207542 34.0234714832235 18.005051681029492 42-43 17.766537417172284 30.0405139264304 32.72170258220132 19.471246074195996 44-45 18.664373330322437 29.037503384168318 32.78788300852872 19.510240276980525 46-47 19.530175401293402 30.159248188338612 32.03053596167428 18.2800404486937 48-49 18.903914168416915 29.900663384153365 32.139991816380345 19.05543063104938 50-51 17.675115470372045 30.30208755268741 32.78142980114249 19.241367175798054 52-53 18.473768488231894 31.426965560607446 31.00793880371549 19.09132714744517 54-55 18.278615501641244 30.127484018140656 31.35209373277062 20.241806747447477 56-57 19.305393820869668 30.21232776801189 30.708657523815177 19.77362088730327 58-59 19.798879799300124 29.844958684769658 30.908621886110733 19.44753962981949 60-61 18.96870891834977 30.643377277391544 31.669764317534625 18.71814948672406 62-63 18.246241410173784 30.408932595104147 31.782645460434466 19.562180534287602 64-65 18.167920120687615 31.249224789750617 31.026499931390028 19.556355158171737 66-67 18.587436730155943 30.335075198550793 30.14502356895697 20.93246450233629 68-69 18.149964643809042 30.224557141044006 30.74459808499661 20.88088013015034 70-71 19.247787428290756 30.234043482524825 30.634939472081502 19.88322961710292 72-73 19.01855684036766 29.162885989614367 31.69921551466243 20.11934165535554 74-75 17.95448682923372 31.50315598970502 30.13016242695155 20.41219475410971 76-77 18.266326874894435 30.052327437359665 30.582539708104495 21.0988059796414 78-79 18.27947374406833 30.104985816736747 30.77356061717386 20.841979822021063 80-81 18.791988346385956 29.820642308635335 30.30631944830769 21.081049896671015 82-83 18.35552574041765 29.851706316862575 31.024276353363227 20.768491589356543 84-85 18.02091136774388 30.039137733670568 30.46640574844219 21.473545150143355 86-87 17.739754451707544 30.293062324318125 30.526713545418016 21.440469678556312 88-89 18.096847415297614 29.986627354177337 29.819096899548757 22.097428330976292 90-91 18.158549682364487 29.02856816628579 30.483080675614627 22.329801475735096 92-93 18.760234612129317 30.011447107194865 30.25575919703776 20.972559083638053 94-95 18.25339725252945 29.199271143153123 30.49347828057692 22.053853323740512 96-97 17.532122362900495 29.278758075402568 30.850036302740957 22.33908325895598 98-99 17.90736830654054 29.79950320837722 29.4551774950428 22.83795099003944 100-101 17.63045498280654 28.8137234500648 30.520377699805714 23.03544386732295 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2708.0 1 1730.0 2 903.5 3 1049.0 4 885.0 5 880.0 6 1489.5 7 2897.5 8 4328.5 9 4629.0 10 3768.5 11 2895.5 12 2785.0 13 2880.0 14 2964.5 15 3111.5 16 3267.0 17 3497.5 18 3743.0 19 4088.5 20 4643.0 21 5243.0 22 5960.5 23 6789.0 24 7759.0 25 9728.5 26 13390.5 27 17099.0 28 18231.0 29 19627.5 30 23512.0 31 27093.0 32 31872.0 33 38118.5 34 44582.0 35 49280.5 36 53834.0 37 58301.0 38 55901.5 39 51315.0 40 48272.5 41 45186.5 42 42039.0 43 40364.0 44 39795.5 45 38811.0 46 37865.5 47 45267.0 48 64845.5 49 60844.5 50 37528.5 51 24794.0 52 17876.5 53 14303.5 54 11887.0 55 10138.0 56 8558.5 57 6904.5 58 5589.0 59 4571.0 60 3588.5 61 2705.5 62 1866.0 63 1259.5 64 874.0 65 572.5 66 377.5 67 284.5 68 210.5 69 148.0 70 108.0 71 93.5 72 86.5 73 81.5 74 80.5 75 61.0 76 34.5 77 26.0 78 23.0 79 20.0 80 16.0 81 9.5 82 5.5 83 3.5 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3467921315766268 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.768880515770883E-4 16-17 0.001648251575934538 18-19 4.1206289398363446E-5 20-21 0.0 22-23 0.0 24-25 4.120628939836345E-4 26-27 0.0010713635243574497 28-29 0.0 30-31 0.003008059126080532 32-33 0.0 34-35 0.009395033982826867 36-37 0.025630312005782065 38-39 0.01957298746422264 40-41 0.012155855372517219 42-43 0.009930715745005592 44-45 0.003008059126080532 46-47 0.0014834264183410841 48-49 6.180943409754517E-4 50-51 4.1206289398363446E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.004367866676226526 60-61 0.010054334613200683 62-63 0.004367866676226526 64-65 0.002183933338113263 66-67 0.004326660386828162 68-69 0.0033789157306658027 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21967072878267555 78-79 4.120628939836345E-4 80-81 0.004079422650437981 82-83 3.2965031518690757E-4 84-85 0.0 86-87 0.11076250590280096 88-89 0.4095905166197327 90-91 0.3946738398575251 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001730664154731265 100-101 7.829194985689055E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1213407.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.15311268862043 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68753348187322 24.825055220642867 2 13.70540947871044 9.361647887401686 3 5.15955943342373 5.286450442600658 4 2.414983910999401 3.299168706142714 5 1.385876844655721 2.366600402403828 6 0.8843557540239072 1.812210103440504 7 0.5854208199803326 1.3995760264537016 8 0.42265605036141995 1.154801577321665 9 0.29770898836200077 0.9150919765148344 >10 1.9034762171462045 13.005056527559134 >50 0.2962802495007416 7.125595701390621 >100 0.2309443592561818 15.242492140505975 >500 0.016392710243572264 3.687350459523671 >1k 0.007955285853498304 5.767567418178721 >5k 9.642770731513096E-4 2.513945405508323 >10k+ 4.821385365756548E-4 2.237390004411105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14579 1.2014929862774815 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12596 1.038068842523572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9783 0.8062422583683794 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7753 0.6389447234110237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6959 0.5735091358464225 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6039 0.49768956335343373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4481 0.36929076558813323 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3978 0.32783723845337964 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3892 0.32074975667686106 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3755 0.3094592333817095 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3619 0.29825112266535464 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3544 0.29207017925560014 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3302 0.27212633518679225 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3261 0.2687474194561264 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2965 0.24435329613229526 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2546 0.2098224256164667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2186 0.180153897249645 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.17306641547312648 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2052 0.1691106116908836 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1985 0.1635889689115029 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1955 0.16111659154760108 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1911 0.1574904380805451 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 0.15353463429830222 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1730 0.14257376131833754 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1670 0.13762900659053393 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1456 0.11999271472803437 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1440 0.11867411346728673 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1374 0.11323488326670277 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1356 0.11175145684836167 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1354 0.11158663169076823 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1306 0.10763082790852534 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.104416737335453 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1261 0.10392226186267263 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.24125787967269E-5 0.0 9 0.0 0.0 0.0 8.24125787967269E-5 0.0 10-11 0.0 0.0 0.0 8.24125787967269E-5 0.0 12-13 0.0 0.0 0.0 8.24125787967269E-5 0.0 14-15 0.0 0.0 0.0 1.2361886819509035E-4 0.0 16-17 0.0 0.0 0.0 3.708566045852711E-4 0.0 18-19 0.0 0.0 0.0 5.768880515770883E-4 0.0 20-21 0.0 0.0 0.0 9.477446561623594E-4 0.0 22-23 0.0 0.0 0.0 0.002101520759316536 0.0 24-25 0.0 0.0 0.0 0.004203041518633072 0.0 26-27 0.0 0.0 0.0 0.007870401275087419 0.0 28-29 0.0 0.0 0.0 0.01969660633241773 0.0 30-31 0.0 0.0 0.0 0.05101338627517395 0.0 32-33 0.0 0.0 0.0 0.10532327570221697 0.0 34-35 0.0 0.0 0.0 0.18584036518661917 0.0 36-37 0.0 0.0 0.0 0.2976330283243792 0.0 38-39 0.0 0.0 0.0 0.45508226011552594 0.0 40-41 0.0 0.0 0.0 0.621184812680329 0.0 42-43 0.0 0.0 0.0 0.7940864029958621 0.0 44-45 0.0 0.0 0.0 1.0025902273515812 0.0 46-47 0.0 0.0 0.0 1.2501164077675504 0.0 48-49 0.0 0.0 0.0 1.4870525718081402 0.0 50-51 0.0 0.0 0.0 1.7581899560493717 0.0 52-53 0.0 0.0 0.0 2.0107433037719415 0.0 54-55 0.0 0.0 0.0 2.2988576792452986 0.0 56-57 0.0 0.0 0.0 2.650594565549729 0.0 58-59 0.0 0.0 0.0 3.0164240028283995 0.0 60-61 0.0 0.0 0.0 3.42255319113867 0.0 62-63 0.0 0.0 0.0 3.8277758410821763 0.0 64-65 0.0 0.0 0.0 4.250099101126003 0.0 66-67 0.0 0.0 0.0 4.7175432480610375 0.0 68-69 0.0 0.0 0.0 5.258870271887339 0.0 70-71 0.0 0.0 0.0 5.855207692060455 0.0 72-73 0.0 0.0 0.0 6.420475570027204 0.0 74-75 0.0 0.0 0.0 7.0372101034525105 0.0 76-77 0.0 0.0 0.0 7.678297553912248 0.0 78-79 0.0 0.0 0.0 8.459651213484015 0.0 80-81 0.0 0.0 0.0 9.291729815305171 0.0 82-83 0.0 0.0 0.0 10.082148858544578 0.0 84-85 0.0 0.0 0.0 10.887649403703787 0.0 86-87 0.0 0.0 0.0 11.736086902416089 0.0 88-89 0.0 0.0 0.0 12.634466423879209 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAATA 25 0.0050446857 56.933247 2 GTAAGCG 85 0.0 39.073444 94-95 TTCGTCG 50 4.9936833E-5 28.467796 46-47 CGTCGGT 35 0.00844064 27.111628 48-49 GAGTACT 8770 0.0 23.18311 12-13 CTATACG 145 2.3557228E-5 22.904179 4 AGGCTTA 520 0.0 21.897402 6 AGTACTT 9005 0.0 21.866781 12-13 CATATAG 420 0.0 21.462929 3 GTACTTT 9340 0.0 21.387287 14-15 CCGTGCA 200 5.447655E-7 21.349968 8 CTATACA 440 0.0 20.487343 4 TGACCGT 210 8.7058834E-7 20.333302 5 GGACAAT 585 0.0 20.275373 6 GTACATG 7340 0.0 20.11157 1 GACCGTG 260 1.5104888E-8 20.072618 6 GTGTAGC 430 0.0 19.933538 1 CATGGGG 2090 0.0 19.74957 4 TATACGT 195 1.0432224E-5 19.464357 5 ACCGTGC 220 1.3600584E-6 19.409061 7 >>END_MODULE