##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193703 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.237894183058934 33.0 31.0 34.0 31.0 34.0 2 32.32708806126817 34.0 31.0 34.0 31.0 34.0 3 32.37024368708129 34.0 31.0 34.0 30.0 34.0 4 35.813505536971924 37.0 35.0 37.0 35.0 37.0 5 35.87544305409302 37.0 35.0 37.0 35.0 37.0 6 35.98684178560329 37.0 37.0 37.0 35.0 37.0 7 35.94899317501925 37.0 37.0 37.0 35.0 37.0 8 35.95463695743413 37.0 37.0 37.0 35.0 37.0 9 37.63043152274896 39.0 38.0 39.0 35.0 39.0 10-11 37.56032363159011 39.0 38.0 39.0 35.0 39.0 12-13 37.50244030550313 39.0 38.0 39.0 35.0 39.0 14-15 38.7515877064898 40.0 38.0 41.0 35.0 41.0 16-17 38.706288331352106 40.0 38.0 41.0 35.0 41.0 18-19 38.721465473405026 40.0 38.5 41.0 35.0 41.0 20-21 38.739050249517675 40.0 38.5 41.0 35.0 41.0 22-23 38.75676403594529 40.5 39.0 41.0 35.0 41.0 24-25 38.69760987448302 41.0 38.5 41.0 35.0 41.0 26-27 38.57262610548855 40.0 38.0 41.0 34.5 41.0 28-29 38.450106936147435 40.0 38.0 41.0 34.0 41.0 30-31 38.206351998780264 40.0 38.0 41.0 34.0 41.0 32-33 37.9249888791433 40.0 37.0 41.0 34.0 41.0 34-35 37.59070053438753 40.0 37.0 41.0 33.0 41.0 36-37 37.30513829654445 40.0 36.5 41.0 33.0 41.0 38-39 37.09855215241982 40.0 36.0 41.0 32.5 41.0 40-41 36.935241429400776 40.0 36.0 41.0 31.5 41.0 42-43 36.66302044981038 40.0 36.0 41.0 31.0 41.0 44-45 36.263761170073295 40.0 36.0 41.0 30.0 41.0 46-47 35.840438115678694 40.0 36.0 41.0 28.0 41.0 48-49 35.552559137406874 40.0 36.0 41.0 25.0 41.0 50-51 34.400817037403776 38.5 34.5 40.0 23.0 40.5 52-53 34.55495629985013 38.5 35.0 40.0 23.0 40.5 54-55 35.105073456295244 40.0 35.0 41.0 23.0 41.0 56-57 35.01862020954961 40.0 35.0 41.0 22.0 41.0 58-59 34.86119914250027 39.0 35.0 41.0 20.5 41.0 60-61 34.568808154122095 39.0 35.0 41.0 18.5 41.0 62-63 34.24586057000778 39.0 35.0 41.0 13.5 41.0 64-65 33.90836037104707 38.5 35.0 40.0 8.0 41.0 66-67 33.49731298321274 38.0 34.0 40.0 4.5 41.0 68-69 33.06132220493707 37.0 34.0 40.0 2.0 41.0 70-71 32.602794413685814 36.5 33.5 39.0 2.0 41.0 72-73 32.125030681836265 36.0 33.0 39.0 2.0 40.5 74-75 31.658074914781984 35.5 32.5 38.5 2.0 40.0 76-77 31.200199295804737 35.0 32.0 37.0 2.0 39.5 78-79 30.723756244224905 35.0 31.5 37.0 2.0 39.0 80-81 30.213337404697818 35.0 31.0 36.0 2.0 39.0 82-83 29.782853858958216 35.0 31.0 36.0 2.0 37.5 84-85 29.355502583138353 35.0 30.5 35.5 2.0 37.0 86-87 28.9711733153054 34.5 30.0 35.0 2.0 36.5 88-89 28.500066599480775 34.0 29.0 35.0 2.0 36.0 90-91 28.053352048206296 34.0 29.0 35.0 2.0 36.0 92-93 27.635703353346685 34.0 28.5 35.0 2.0 35.5 94-95 27.159245222639132 34.0 26.5 35.0 2.0 35.0 96-97 26.578586968450274 34.0 25.0 35.0 2.0 35.0 98-99 25.97836522149982 34.0 24.0 35.0 2.0 35.0 100-101 23.267522993575454 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2765409233332363 1101 2 0.36356459193473256 1101 3 0.23716251112810482 1101 4 0.3118081698866533 1101 5 0.19915521698995065 1101 6 0.1616343761341028 1101 7 0.18366024478578424 1101 8 0.21849097196263756 1101 9 0.3691026526889729 1101 10-11 0.28615235347709245 1101 12-13 0.2328252866108187 1101 14-15 0.4472641094544798 1101 16-17 0.44248084168462753 1101 18-19 0.5210983531709203 1101 20-21 0.5324072111229512 1101 22-23 0.5333340578512349 1101 24-25 0.48725040637731354 1101 26-27 0.49754012146953386 1101 28-29 0.427516966276265 1101 30-31 0.6232066566562153 1101 32-33 0.4759703252469194 1101 34-35 0.5883422414182249 1101 36-37 0.4823915163653538 1101 38-39 0.5003999637441865 1101 40-41 0.46580084505431785 1101 42-43 0.5558967547930607 1101 44-45 0.5824645253369667 1101 46-47 0.8333043687485215 1101 48-49 0.6219776373737957 1101 50-51 0.5505438347406653 1101 52-53 0.6135154625154868 1101 54-55 0.7151180334318425 1101 56-57 0.6068279169836472 1101 58-59 0.6389352779190247 1101 60-61 0.5896837959618608 1101 62-63 0.6014884584498006 1101 64-65 0.5327352344274274 1101 66-67 0.7106894150069607 1101 68-69 0.585910731389923 1101 70-71 0.6635584980589257 1101 72-73 0.457224444528876 1101 74-75 0.46256267701819453 1101 76-77 0.47538746072355664 1101 78-79 0.34405051961148203 1101 80-81 0.3269850084514836 1101 82-83 0.25055172763610756 1101 84-85 0.16195898294184374 1101 86-87 0.16177991047590012 1101 88-89 0.1972541292163683 1101 90-91 0.1808422150016007 1101 92-93 -0.06269844247886525 1101 94-95 -0.19064240107691433 1101 96-97 -0.4223320640220187 1101 98-99 -0.7498711219234657 1101 100-101 -0.9712069984104055 1102 1 0.3519856023353469 1102 2 0.37082765730232126 1102 3 0.4395683327805031 1102 4 0.2965880329054258 1102 5 0.41730018029461746 1102 6 0.4303557946268697 1102 7 0.3861470671671867 1102 8 0.3539921914736155 1102 9 0.5306831069130595 1102 10-11 0.5517012434170994 1102 12-13 0.5042337292868027 1102 14-15 0.6191406563434043 1102 16-17 0.703349189499761 1102 18-19 0.666674163353214 1102 20-21 0.6055408693258713 1102 22-23 0.6893179617063652 1102 24-25 0.7373526572975706 1102 26-27 0.6461349588230618 1102 28-29 0.7514263814609947 1102 30-31 0.8184519138635267 1102 32-33 0.7543688308334708 1102 34-35 0.7711648868220422 1102 36-37 0.7415803490648472 1102 38-39 0.6205920454729252 1102 40-41 0.3545063434038056 1102 42-43 0.42500857679946336 1102 44-45 0.31829564781707376 1102 46-47 0.5214635621812604 1102 48-49 0.5523455106846527 1102 50-51 0.5252226068496171 1102 52-53 0.5067420015543362 1102 54-55 0.5771092913531746 1102 56-57 0.6286441133294147 1102 58-59 0.548604464941711 1102 60-61 0.4712257298392686 1102 62-63 0.5488434846450403 1102 64-65 0.46108958961662694 1102 66-67 0.7003218216986227 1102 68-69 0.5552219551909374 1102 70-71 0.544802429254311 1102 72-73 0.643753623291488 1102 74-75 0.5033040436904948 1102 76-77 0.5194858576091299 1102 78-79 0.4512669845596662 1102 80-81 0.40291319180513696 1102 82-83 0.3345159833144109 1102 84-85 0.4428405108755946 1102 86-87 0.6524758267391455 1102 88-89 0.42259665729912044 1102 90-91 0.7091924701538979 1102 92-93 0.5675812300381899 1102 94-95 0.4104455087142753 1102 96-97 0.5736518998336564 1102 98-99 0.1596375636547016 1102 100-101 -0.01209486640121682 1103 1 0.2702586841672172 1103 2 0.26971583219224726 1103 3 0.3345652683057381 1103 4 0.36869279734682436 1103 5 0.2664626727076964 1103 6 0.40988173671770056 1103 7 0.1812262830973097 1103 8 0.3479830485463111 1103 9 0.3699323452499854 1103 10-11 0.42114372683972334 1103 12-13 0.4241806381278366 1103 14-15 0.6054396322444475 1103 16-17 0.6099936044821206 1103 18-19 0.6699317148824306 1103 20-21 0.5134630564202283 1103 22-23 0.47917816422904025 1103 24-25 0.6869016992902814 1103 26-27 0.6726815284430785 1103 28-29 0.6105744079689543 1103 30-31 0.5801669701874914 1103 32-33 0.5487519802818142 1103 34-35 0.5235607714155961 1103 36-37 0.2793456687158056 1103 38-39 0.4812629030604967 1103 40-41 0.4645843563171752 1103 42-43 0.5277551184976019 1103 44-45 0.39634470119636234 1103 46-47 0.4637836121633896 1103 48-49 0.4188240182705911 1103 50-51 0.6733560496261219 1103 52-53 0.5510823275669239 1103 54-55 0.4316279311818363 1103 56-57 0.4308641990554989 1103 58-59 0.40211365985121716 1103 60-61 0.453459202220877 1103 62-63 0.45405022035067333 1103 64-65 0.33995916972912 1103 66-67 0.31983138691946067 1103 68-69 0.418086956908823 1103 70-71 0.4955752808792724 1103 72-73 0.49495134280738284 1103 74-75 0.3988589215521152 1103 76-77 0.3049924266746693 1103 78-79 0.18446707842467447 1103 80-81 0.37255855434883856 1103 82-83 0.3716238518360697 1103 84-85 0.23297102086558752 1103 86-87 0.17983876451594938 1103 88-89 0.33008318400658965 1103 90-91 0.4482571469087411 1103 92-93 0.2624268725927017 1103 94-95 0.24653323883454448 1103 96-97 0.349474431594782 1103 98-99 0.15473539416499094 1103 100-101 -0.07277790267687223 1104 1 0.17723618853101897 1104 2 0.29433896701196716 1104 3 0.42430779240820016 1104 4 0.4343912248843864 1104 5 0.43055090693053444 1104 6 0.3969822586926739 1104 7 0.3966079673701586 1104 8 0.4012228142786398 1104 9 0.34558191876963207 1104 10-11 0.5016015593127463 1104 12-13 0.44536269802841133 1104 14-15 0.4872373779177721 1104 16-17 0.4989882353032087 1104 18-19 0.5279557374985444 1104 20-21 0.5142158061514834 1104 22-23 0.5440780793345681 1104 24-25 0.6687381097761076 1104 26-27 0.698257741551572 1104 28-29 0.6938942771063026 1104 30-31 0.6431350455670639 1104 32-33 0.6178153672259938 1104 34-35 0.3773984408426614 1104 36-37 0.5266664493714472 1104 38-39 0.5084571545181262 1104 40-41 0.6925232322918475 1104 42-43 0.5724148447992974 1104 44-45 0.6812226502055054 1104 46-47 0.831078784385241 1104 48-49 0.79965887936212 1104 50-51 0.571553652968106 1104 52-53 0.5241855336643866 1104 54-55 0.4798790955623886 1104 56-57 0.6127345112972051 1104 58-59 0.48618067512381913 1104 60-61 0.6052223270101535 1104 62-63 0.6094197261415317 1104 64-65 0.5307261245762831 1104 66-67 0.5413690883804065 1104 68-69 0.5451069365304093 1104 70-71 0.3244839525374985 1104 72-73 0.3132071114760038 1104 74-75 0.19617451888143833 1104 76-77 0.1043421293572031 1104 78-79 0.16519210903432224 1104 80-81 0.29973468751269294 1104 82-83 0.29793936604538374 1104 84-85 0.2141349998296853 1104 86-87 0.17028089758080966 1104 88-89 0.3188260265800942 1104 90-91 0.5858688569301194 1104 92-93 0.5517769581876344 1104 94-95 0.2094883325500163 1104 96-97 0.4381560081851994 1104 98-99 0.5490679171560764 1104 100-101 0.5915998506733757 1105 1 0.13939323678078352 1105 2 0.312314369093329 1105 3 0.24360769780082592 1105 4 0.22814713784559615 1105 5 0.359595372398843 1105 6 0.25790941101055154 1105 7 0.28402518591320813 1105 8 0.266880335565304 1105 9 0.26611172009413764 1105 10-11 0.3038721364177661 1105 12-13 0.3185888096651226 1105 14-15 0.2947113608884422 1105 16-17 0.2487517168547697 1105 18-19 0.4177381405259837 1105 20-21 0.3533832612129757 1105 22-23 0.5067081644812106 1105 24-25 0.4766851296436556 1105 26-27 0.49910920796595093 1105 28-29 0.4256823565765089 1105 30-31 0.5267679689350828 1105 32-33 0.37183618084946346 1105 34-35 0.5141061040403869 1105 36-37 0.5682936962967062 1105 38-39 0.510822339002523 1105 40-41 0.5789943770411412 1105 42-43 0.5118661219989207 1105 44-45 0.5568139463265993 1105 46-47 0.2610693236472983 1105 48-49 0.3923741111501968 1105 50-51 0.35868704937302454 1105 52-53 0.2947626386785842 1105 54-55 0.30343633302691586 1105 56-57 0.3946645018364734 1105 58-59 0.4880728227113309 1105 60-61 0.44344370827789703 1105 62-63 0.44622199671862717 1105 64-65 0.44226822486010775 1105 66-67 0.4387200817578929 1105 68-69 0.30906152498831574 1105 70-71 0.22467316729625253 1105 72-73 0.2573887576444065 1105 74-75 0.20374310923148897 1105 76-77 0.12468271592295466 1105 78-79 0.13822900591227594 1105 80-81 0.3224404585628342 1105 82-83 0.28469433293280133 1105 84-85 0.22548788535475595 1105 86-87 0.23792517383435907 1105 88-89 0.37180615903042735 1105 90-91 0.1582435021524482 1105 92-93 0.08252435648470069 1105 94-95 0.06946941107414517 1105 96-97 -0.023054966270809274 1105 98-99 0.21084653588834001 1105 100-101 0.04098490270743227 1106 1 0.19105918672487832 1106 2 0.31577613911138513 1106 3 0.2658799950400095 1106 4 0.25331184069241885 1106 5 0.18649139028823924 1106 6 0.16091018507092514 1106 7 0.13709992574291618 1106 8 0.2851292389797706 1106 9 0.18769596366513497 1106 10-11 0.29668756602127644 1106 12-13 0.3034860316484327 1106 14-15 0.2718082044422374 1106 16-17 0.33697825852502206 1106 18-19 0.2970088036377021 1106 20-21 0.31836433973710854 1106 22-23 0.32052385210484147 1106 24-25 0.31874456027924936 1106 26-27 0.32709192061754067 1106 28-29 0.469872173382214 1106 30-31 0.4373773264649685 1106 32-33 0.4178428893727286 1106 34-35 0.6091611483338468 1106 36-37 0.5493016949206009 1106 38-39 0.5813611710314177 1106 40-41 0.7107523541633611 1106 42-43 0.694239907154163 1106 44-45 0.5426172489842074 1106 46-47 0.6403971812201874 1106 48-49 0.703425113128354 1106 50-51 0.7341484753642504 1106 52-53 0.7349479831354486 1106 54-55 0.6593888573238118 1106 56-57 0.7339760181347401 1106 58-59 0.7334508221952944 1106 60-61 0.8478597227270228 1106 62-63 0.7300858480990371 1106 64-65 0.8663380623074275 1106 66-67 0.6904075333791155 1106 68-69 0.7026351037274523 1106 70-71 0.6059495068507275 1106 72-73 0.6889592401420401 1106 74-75 0.7575540664861542 1106 76-77 0.9630068752073804 1106 78-79 0.8709752001154243 1106 80-81 0.6985852078532808 1106 82-83 0.9551579090917457 1106 84-85 1.036842060980458 1106 86-87 1.15977818836949 1106 88-89 1.2360191976474404 1106 90-91 1.1395202717404729 1106 92-93 1.0438596106345237 1106 94-95 1.0197953764994452 1106 96-97 1.1566659419600356 1106 98-99 1.268587139655434 1106 100-101 1.2851149448507222 1107 1 0.2579436901218344 1107 2 0.17803754318774168 1107 3 0.14765611927164457 1107 4 0.2747547394306906 1107 5 0.16900190468487608 1107 6 0.20304218118869244 1107 7 0.21058747022689772 1107 8 0.2584582587144837 1107 9 0.24549926356807816 1107 10-11 0.24841192706947623 1107 12-13 0.1721935696620207 1107 14-15 0.2360139630963971 1107 16-17 0.2281607237466261 1107 18-19 0.364021097393767 1107 20-21 0.3574924536161106 1107 22-23 0.24405021897640466 1107 24-25 0.25213990159273436 1107 26-27 0.2634749681749682 1107 28-29 0.2134066282511924 1107 30-31 0.2846387305413174 1107 32-33 0.2004423717408912 1107 34-35 0.3217088675185735 1107 36-37 0.3564628078290397 1107 38-39 0.5424206986923679 1107 40-41 0.6084068349139216 1107 42-43 0.5475867204831815 1107 44-45 0.5287932438078897 1107 46-47 0.5596595558562356 1107 48-49 0.5457188691943458 1107 50-51 0.3792325905373346 1107 52-53 0.43592501322279276 1107 54-55 0.41936782853053245 1107 56-57 0.5284114759153979 1107 58-59 0.5345815242334666 1107 60-61 0.2902214179712814 1107 62-63 0.19437572192627783 1107 64-65 0.2748935459696682 1107 66-67 0.1844757960772725 1107 68-69 0.2587609529671866 1107 70-71 0.36305076857832574 1107 72-73 0.37717988071143793 1107 74-75 0.27974591378476177 1107 76-77 0.3254915080122949 1107 78-79 0.3965753295233796 1107 80-81 0.3078200832981217 1107 82-83 0.3975422507287547 1107 84-85 0.5095709513072144 1107 86-87 0.33918452935946064 1107 88-89 0.3146701196791426 1107 90-91 0.34008157876085576 1107 92-93 0.16475576074775589 1107 94-95 0.3670470749789061 1107 96-97 0.4877335576183981 1107 98-99 0.5723561464493478 1107 100-101 0.7365465203926185 1108 1 -0.039324034803385643 1108 2 -0.030337670742881073 1108 3 0.035783611355839184 1108 4 0.009674705986583376 1108 5 -0.011522334748384822 1108 6 0.08627605753603973 1108 7 -0.0943674194648878 1108 8 -0.03944937155304018 1108 9 -0.013261704620781245 1108 10-11 0.026130468326769574 1108 12-13 0.11852188637894301 1108 14-15 0.088553134660458 1108 16-17 0.05117596073799291 1108 18-19 0.03120881905909556 1108 20-21 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2316 52-53 -1.6316649758776336 2316 54-55 -1.544724822274965 2316 56-57 -1.5969270198545331 2316 58-59 -1.2664402252480684 2316 60-61 -1.3896075626825564 2316 62-63 -1.2587355619430411 2316 64-65 -1.4075283122818405 2316 66-67 -1.6582446831424704 2316 68-69 -1.6689496083265034 2316 70-71 -1.6621141352775908 2316 72-73 -2.0224358137577703 2316 74-75 -1.8983956654559684 2316 76-77 -1.8428901098620862 2316 78-79 -1.5267615843429851 2316 80-81 -1.756509909458071 2316 82-83 -1.6567507782893571 2316 84-85 -1.4900510617496998 2316 86-87 -1.6747744720328832 2316 88-89 -1.7539793611811767 2316 90-91 -1.752599691365539 2316 92-93 -1.864029073287771 2316 94-95 -1.8798610482425815 2316 96-97 -2.0965775583570228 2316 98-99 -2.5028632552255274 2316 100-101 -2.5253998415676797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4183.0 3 723.0 4 603.0 5 706.0 6 778.0 7 914.0 8 1266.0 9 1545.0 10 1982.0 11 3025.0 12 4837.0 13 8647.0 14 7801.0 15 9208.0 16 12664.0 17 20832.0 18 16217.0 19 8563.0 20 7423.0 21 7163.0 22 7348.0 23 7876.0 24 8737.0 25 9526.0 26 11017.0 27 12474.0 28 14211.0 29 16988.0 30 20285.0 31 24641.0 32 30223.0 33 38926.0 34 53868.0 35 82676.0 36 126824.0 37 235615.0 38 309992.0 39 63386.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.207252099391525 22.209943357428504 15.752101494068722 19.830703049111253 2 14.033151302827918 23.70771900484026 40.86208819826703 21.39704149406479 3 18.922600266045336 28.600712249995386 32.53871999651088 19.937967487448397 4 10.872769214637728 19.419775610779073 42.134088287721504 27.573366886861695 5 10.449736892215986 40.52237359879272 36.06745088696641 12.960438622024887 6 25.09683196008008 41.203130816205395 18.99474173113747 14.705295492577056 7 23.440904932910637 36.83194666054032 23.642219134826853 16.08492927172219 8 23.43396283365697 34.28269829930946 21.801856640272593 20.481482226760974 9 24.855789869070616 18.86805108392359 24.633857185018243 31.642301861987548 10-11 22.305109017212274 28.50385818351178 29.217141091677078 19.973891707598863 12-13 24.91475045590794 27.702056302010185 28.87060599073511 18.512587251346762 14-15 21.815345765573767 28.575680418257164 29.954123171571123 19.654850644597946 16-17 17.805591409028203 31.529196837032032 30.699175554112568 19.966036199827204 18-19 17.433816238787696 30.807537266032618 33.92373433841394 17.834912156765743 20-21 19.448566958289103 28.568979019618695 33.287804010940455 18.69465001115175 22-23 18.651506937307836 28.769439703010857 33.44276715794487 19.136286201736432 24-25 19.11657587078707 29.323469703977896 33.50617469292178 18.053779732313256 26-27 18.69797428372776 28.377221196676395 35.24346252974246 17.681341989853387 28-29 17.415415374280705 30.12263916360317 34.427652454020276 18.034293008095855 30-31 18.425760736260628 28.894665674364 33.93364849425058 18.74592509512479 32-33 18.332215271605147 29.451213376490514 33.962676304358766 18.253895047545576 34-35 19.21769572423104 28.87996600467982 33.823241988423604 18.07909628266554 36-37 18.952095997506994 28.269783483425943 34.36172564741256 18.4163948716545 38-39 18.06992326276 29.94903832146445 33.85558107007736 18.125457345698194 40-41 18.1168433480866 29.88367859345174 33.87893060913524 18.12054744932642 42-43 17.821078088764562 30.030794596588255 32.54004476458175 19.60808255006543 44-45 18.70553157050587 29.040587251706313 32.704510036174774 19.54937114161304 46-47 19.528512774779124 30.138828010491153 31.989222523561516 18.343436691168204 48-49 18.85654947811942 29.83185368563027 32.21881465080722 19.092782185443095 50-51 17.65491581207054 30.325129715858896 32.787394001867945 19.23256047020262 52-53 18.490865880197 31.42361228078449 30.951536782391344 19.133985056627164 54-55 18.32534583711234 30.009238042545523 31.502525093033068 20.16289102730907 56-57 19.274860733086552 30.130987088187084 30.829952509919373 19.764199668806988 58-59 19.87368864102699 29.676477087388857 31.034713526595464 19.415120744988688 60-61 18.981091769722347 30.401078955859482 31.925827674157688 18.692001600260483 62-63 18.304369058745273 30.151388853806193 32.037434640935615 19.50680744651292 64-65 18.278163934094867 30.957650822045522 31.375474483784384 19.388710760075227 66-67 18.659081461915132 30.016210090181 30.460776761915508 20.86393168598836 68-69 18.25726036213778 29.869922548274648 31.203298564017718 20.669518525569853 70-71 19.387942918588525 29.894832962194368 31.03112049710579 19.686103622111318 72-73 19.119764470941885 28.791751260965675 32.14023260642247 19.94825166166997 74-75 18.11823636829196 31.158821527721624 30.682587733484272 20.040354370502143 76-77 18.501252212635304 29.75849222482901 30.953610027132676 20.786645535403007 78-79 18.674385115046384 29.69426340299553 31.172033084596904 20.45931839736118 80-81 19.20779066605261 29.42708311407737 30.751122520372913 20.614003699497108 82-83 18.765446548940982 29.3018559857456 31.676466848495615 20.256230616817803 84-85 18.47825949672184 29.49857847559874 31.180073650667484 20.84308837701193 86-87 18.37855361071056 29.74943426412235 31.147954404319588 20.7240577208475 88-89 18.690315789473686 29.339747368421055 30.58345263157895 21.386484210526316 90-91 18.892745454591395 28.33162216362039 31.28454801115475 21.491084370633466 92-93 19.67894556410686 29.082312946371037 31.168627702021457 20.07011378750064 94-95 19.243811147346577 28.258075492547775 31.36623049349109 21.13188286661456 96-97 18.62655420623249 28.127953223467106 31.868071242910155 21.37742132739025 98-99 19.17173508931963 28.639851826021918 30.523948981388127 21.664464103270323 100-101 19.20361020832044 27.30784242643186 31.67559492607081 21.81295243917689 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3712.0 1 2250.0 2 946.5 3 1102.0 4 936.5 5 938.5 6 1555.5 7 2892.5 8 4447.0 9 4865.5 10 3947.5 11 3225.5 12 3227.5 13 3289.0 14 3398.5 15 3570.0 16 3684.5 17 3794.0 18 3909.5 19 4162.5 20 4633.5 21 5287.5 22 5919.5 23 6655.0 24 7506.0 25 9317.0 26 13065.5 27 16825.5 28 17751.0 29 19045.5 30 22961.5 31 26504.0 32 31063.5 33 37279.5 34 43898.0 35 48369.5 36 52506.5 37 57152.0 38 55007.5 39 50229.5 40 46967.5 41 44085.0 42 41187.5 43 39521.5 44 38818.0 45 37285.0 46 37082.5 47 43976.5 48 63414.5 49 60249.5 50 35455.5 51 22579.5 52 17220.0 53 14443.5 54 12030.0 55 10094.5 56 8471.0 57 6935.0 58 5704.0 59 4715.0 60 3732.5 61 2922.5 62 2081.0 63 1436.5 64 1046.5 65 744.5 66 523.5 67 384.5 68 301.0 69 229.0 70 185.5 71 155.0 72 133.5 73 122.0 74 115.5 75 102.5 76 72.0 77 46.5 78 40.5 79 33.0 80 21.5 81 17.0 82 15.0 83 11.0 84 4.0 85 3.0 86 3.5 87 2.5 88 2.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39105204560933493 2 0.11828737969159833 3 0.11937642780490625 4 0.1321936863692225 5 0.13453932845942418 6 0.07665223259051875 7 0.08712384906463333 8 0.08469443404263875 9 0.08368915886112374 10-11 0.08197181375936896 12-13 0.08611857388311833 14-15 0.07908164761251334 16-17 0.08063144685068228 18-19 0.0917732467791402 20-21 0.08921817235945624 22-23 0.10065317754918937 24-25 0.11531344061294979 26-27 0.11594173760139666 28-29 0.10911424366027395 30-31 0.09973167529946729 32-33 0.09742791967516208 34-35 0.3457727759752635 36-37 0.5345131913047048 38-39 0.51470089293568 40-41 0.4884380788186006 42-43 0.5157899410489879 44-45 0.5255494876028627 46-47 0.5216121598085957 48-49 0.5289841778063723 50-51 0.527769470295375 52-53 0.526680422182067 54-55 0.5212351816155275 56-57 0.49224974721517833 58-59 0.4829928382520611 60-61 0.4311373934722456 62-63 0.5054020975066662 64-65 0.53279584620295 66-67 0.5428067115522035 68-69 0.5563360400367595 70-71 0.5521892799130101 72-73 0.5376965627128356 74-75 0.5420946416319637 76-77 0.5198110417750479 78-79 0.4942184111123118 80-81 0.5004594945308842 82-83 0.5143658012085084 84-85 0.5084598095171077 86-87 0.5102190410847589 88-89 0.5196434959114621 90-91 0.5366494010654241 92-93 0.5436863273360291 94-95 0.5367331739972171 96-97 0.5395814536781762 98-99 0.5403354100643125 100-101 0.5178423778779143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1193703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.62957451755337 #Duplication Level Percentage of deduplicated Percentage of total 1 74.18338601554304 26.43122480005217 2 13.045033039928262 9.295779535601396 3 4.795705044354431 5.1260679072609845 4 2.3441852742313776 3.3408929564471257 5 1.2729489701819057 2.2677315095069517 6 0.8225969189012956 1.7585266931942112 7 0.5275128161370567 1.3156540034063782 8 0.3821991625396752 1.089407483380308 9 0.3019926440631309 0.9683882473860265 >10 1.8034338202324047 12.867033602854564 >50 0.2825383233726601 7.155652783029052 >100 0.21513958211908077 14.687883909842666 >500 0.01437405749635972 3.3287521771967685 >1k 0.0075404891784182125 5.646738427706897 >5k 9.425611473022766E-4 2.5105010997411665 >10k+ 4.712805736511383E-4 2.209764863393335 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13461 1.127667434864451 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12859 1.0772361299251154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9072 0.75998803722534 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8137 0.6816603459989629 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6453 0.5405867288596912 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6240 0.5227430943878 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4760 0.3987591553342833 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3805 0.3187560054720479 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3614 0.3027553754996008 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3519 0.2947969469792737 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3344 0.28013668391551333 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3294 0.2759480373258675 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3288 0.27544539973510995 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3065 0.25676403594528957 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2895 0.24252263754049375 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2372 0.19870939421279835 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2141 0.17935784696863458 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2112 0.17692843194664 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2092 0.17525297331078166 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.16461381097308125 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1937 0.1622681688828796 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.15749311177068334 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1793 0.15020486670469957 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1793 0.15020486670469957 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1683 0.14098984420747876 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1482 0.1241514849171025 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1467 0.12289489094020874 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1400 0.11728210451008333 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1313 0.10999385944409958 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1250 0.10471616474114583 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1215 0.10178411212839375 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6754586358583333E-4 0.0 16-17 0.0 0.0 0.0 2.9320526127520833E-4 0.0 18-19 0.0 0.0 0.0 5.026375907575E-4 0.0 20-21 0.0 0.0 0.0 9.633887156185417E-4 0.0 22-23 0.0 0.0 0.0 0.0016754586358583333 0.0 24-25 0.0 0.0 0.0 0.0033090308058202084 0.0 26-27 0.0 0.0 0.0 0.006366742816261666 0.0 28-29 0.0 0.0 0.0 0.017676088608305416 0.0 30-31 0.0 0.0 0.0 0.047289819997101454 0.0 32-33 0.0 0.0 0.0 0.10195165799197958 0.0 34-35 0.0 0.0 0.0 0.17512731391309228 0.0 36-37 0.0 0.0 0.0 0.28574947034563875 0.0 38-39 0.0 0.0 0.0 0.4411063723556027 0.0 40-41 0.0 0.0 0.0 0.6180348043022427 0.0 42-43 0.0 0.0 0.0 0.7948375768511933 0.0 44-45 0.0 0.0 0.0 1.0029714258906948 0.0 46-47 0.0 0.0 0.0 1.2591071648475374 0.0 48-49 0.0 0.0 0.0 1.513022921111868 0.0 50-51 0.0 0.0 0.0 1.7756510622826616 0.0 52-53 0.0 0.0 0.0 2.030865298989782 0.0 54-55 0.0 0.0 0.0 2.313892149052151 0.0 56-57 0.0 0.0 0.0 2.668628628729257 0.0 58-59 0.0 0.0 0.0 3.031030331665414 0.0 60-61 0.0 0.0 0.0 3.426229137398499 0.0 62-63 0.0 0.0 0.0 3.833198040048488 0.0 64-65 0.0 0.0 0.0 4.250177808047731 0.0 66-67 0.0 0.0 0.0 4.733380078629274 0.0 68-69 0.0 0.0 0.0 5.289841778063723 0.0 70-71 0.0 0.0 0.0 5.887687305803873 0.0 72-73 0.0 0.0 0.0 6.450390088656894 0.0 74-75 0.0 0.0 0.0 7.071231286174199 0.0 76-77 0.0 0.0 0.0 7.714314197082524 0.0 78-79 0.0 0.0 0.0 8.495371126653783 0.0 80-81 0.0 0.0 0.0 9.318859046178154 0.0 82-83 0.0 0.0 0.0 10.106743469690535 0.0 84-85 0.0 0.0 0.0 10.904806304415755 0.0 86-87 0.0 0.0 0.0 11.741446574231613 0.0 88-89 0.0 0.0 0.0 12.647031966913042 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 105 0.0 40.77063 94-95 CATTCCG 365 0.0 28.533358 9 GTACATG 6985 0.0 24.812025 1 CTACACG 335 0.0 24.038109 4 CGCAGTG 100 0.0016865942 23.669716 6 TACATGG 7250 0.0 23.65194 2 ACATGGG 7090 0.0 23.116714 3 GAGTACT 8425 0.0 23.039518 12-13 ACACGGA 370 0.0 23.029993 6 CATATAG 335 0.0 22.624104 3 ACGGAGG 380 0.0 22.423943 8 CCCTATA 275 5.4569682E-11 22.392721 2 TCGCCGG 85 3.1137315E-6 22.383877 94-95 CATGGGG 2035 0.0 22.346165 4 CCTATAC 425 0.0 22.291397 3 TACACGG 365 0.0 22.062374 5 GGCATTC 505 0.0 21.560534 7 AGTACTT 8740 0.0 21.369532 12-13 TTAGGCA 555 0.0 21.337486 4 GTACTTT 9070 0.0 21.323692 14-15 >>END_MODULE