##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193703 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45463402538152 34.0 31.0 34.0 31.0 34.0 2 32.657800977295025 34.0 31.0 34.0 31.0 34.0 3 32.74625765370448 34.0 31.0 34.0 31.0 34.0 4 36.2090402721615 37.0 35.0 37.0 35.0 37.0 5 36.184285370816696 37.0 35.0 37.0 35.0 37.0 6 36.25596651763462 37.0 37.0 37.0 35.0 37.0 7 36.21987965180619 37.0 37.0 37.0 35.0 37.0 8 36.209684486006985 37.0 37.0 37.0 35.0 37.0 9 37.92641553217174 39.0 38.0 39.0 35.0 39.0 10-11 37.85857830632913 39.0 38.0 39.0 35.0 39.0 12-13 37.8012039008028 39.0 38.0 39.0 35.0 39.0 14-15 39.1209647625917 40.5 38.5 41.0 36.0 41.0 16-17 39.23527669780506 41.0 39.0 41.0 36.0 41.0 18-19 39.34675166268326 41.0 39.0 41.0 36.0 41.0 20-21 39.34687941640425 41.0 39.0 41.0 36.0 41.0 22-23 39.26294982922887 41.0 39.0 41.0 36.0 41.0 24-25 39.18090597074817 41.0 39.0 41.0 35.0 41.0 26-27 39.00833875763067 40.5 38.5 41.0 35.0 41.0 28-29 38.86122594983844 40.0 38.0 41.0 35.0 41.0 30-31 38.61647746550021 40.0 38.0 41.0 35.0 41.0 32-33 38.37506104952405 40.0 38.0 41.0 34.0 41.0 34-35 38.19137381744035 40.0 38.0 41.0 34.0 41.0 36-37 37.995941201454634 40.0 37.5 41.0 33.5 41.0 38-39 37.82639232706963 40.0 37.0 41.0 33.0 41.0 40-41 37.64463480446979 40.0 37.0 41.0 33.0 41.0 42-43 37.35938001328638 40.0 37.0 41.0 33.0 41.0 44-45 36.958219925726915 40.0 37.0 41.0 31.5 41.0 46-47 36.43712841468941 40.0 36.5 41.0 30.5 41.0 48-49 35.928289532655945 40.0 36.0 41.0 28.5 41.0 50-51 35.62980448235449 40.0 36.0 41.0 27.0 41.0 52-53 35.40255155595655 39.5 35.0 41.0 26.5 41.0 54-55 35.203582884519854 39.0 35.0 41.0 26.0 41.0 56-57 34.993841851783905 39.0 35.0 41.0 24.5 41.0 58-59 34.70703432930972 39.0 35.0 40.0 23.0 41.0 60-61 34.3320955882661 38.5 35.0 40.0 22.0 41.0 62-63 33.98032173832184 38.0 34.0 40.0 20.0 41.0 64-65 33.56716201601236 38.0 34.0 40.0 17.5 41.0 66-67 33.12269467363322 37.0 33.5 39.5 11.5 41.0 68-69 32.61581188955712 36.5 33.0 39.0 7.5 40.0 70-71 32.10009148004152 36.0 32.0 39.0 4.0 40.0 72-73 31.5200657114877 35.0 31.5 38.0 2.0 40.0 74-75 30.98593913226322 35.0 31.0 37.0 2.0 39.0 76-77 28.964289274635313 33.0 28.5 35.5 2.0 37.5 78-79 29.88733545949034 34.0 30.0 36.0 2.0 38.0 80-81 29.83656613077122 34.0 30.0 36.0 2.0 37.5 82-83 29.4321749212325 34.0 30.0 35.5 2.0 37.0 84-85 28.954791937357953 34.0 29.0 35.0 2.0 36.5 86-87 28.32403537563364 34.0 29.0 35.0 2.0 36.0 88-89 27.64026604607679 33.5 27.0 35.0 2.0 36.0 90-91 27.089781126461105 33.0 26.0 35.0 2.0 35.0 92-93 26.69518590470159 33.0 25.0 35.0 2.0 35.0 94-95 26.211687077941498 32.5 25.0 34.5 2.0 35.0 96-97 25.48411330121479 32.0 22.0 34.0 2.0 35.0 98-99 24.43990423078437 32.0 10.0 34.0 2.0 35.0 100-101 18.853608896015174 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15598561996505822 1101 2 0.20354860752994597 1101 3 0.2382312749842086 1101 4 0.17389382364520145 1101 5 0.2558756214768323 1101 6 0.23737728183870388 1101 7 0.18576303373684766 1101 8 0.19067051073488983 1101 9 0.253936625319902 1101 10-11 0.26432212221290285 1101 12-13 0.2829892052122176 1101 14-15 0.25148317635196804 1101 16-17 0.3353347646936271 1101 18-19 0.35029404335525527 1101 20-21 0.44081755911535225 1101 22-23 0.2934012424244372 1101 24-25 0.4099439017377975 1101 26-27 0.42315841297006074 1101 28-29 0.408459038418151 1101 30-31 0.5174212055121714 1101 32-33 0.5917800734022052 1101 34-35 0.6214549380286272 1101 36-37 0.7104119563702014 1101 38-39 0.685532004910641 1101 40-41 0.7429408193164946 1101 42-43 0.9179045794790852 1101 44-45 0.7611251485760704 1101 46-47 0.7430888919609302 1101 48-49 0.7751652240664058 1101 50-51 0.9463780719546122 1101 52-53 0.8022114000569474 1101 54-55 0.7162987745777727 1101 56-57 0.7677545412974283 1101 58-59 0.7628381740914563 1101 60-61 0.6561455559985205 1101 62-63 0.5433291562028728 1101 64-65 0.5295292052141249 1101 66-67 0.6815024775475464 1101 68-69 0.8173330846814224 1101 70-71 0.7559185758005711 1101 72-73 0.8861060964629672 1101 74-75 0.8289059212933658 1101 76-77 0.9624645944236043 1101 78-79 0.9321032917374481 1101 80-81 0.8214209463079705 1101 82-83 0.7765188496406559 1101 84-85 0.8278034010131883 1101 86-87 0.9643187219047142 1101 88-89 0.7538773797761706 1101 90-91 1.019300649050269 1101 92-93 1.1235704354526206 1101 94-95 1.1171405120199474 1101 96-97 0.9333287121056024 1101 98-99 0.967790125392149 1101 100-101 0.19465397236577076 1102 1 0.11364523890162559 1102 2 0.1507021319116646 1102 3 0.15203849924922963 1102 4 0.15320663746052787 1102 5 0.1452656259868732 1102 6 0.14014240672482714 1102 7 0.19331010166045814 1102 8 0.24633801174240233 1102 9 0.24914858222751235 1102 10-11 0.24864848115013416 1102 12-13 0.2585379851512357 1102 14-15 0.28300646006152164 1102 16-17 0.23809038949425343 1102 18-19 0.33056886582865985 1102 20-21 0.36049083617484 1102 22-23 0.29792028309579877 1102 24-25 0.3214171049966268 1102 26-27 0.31204691296655795 1102 28-29 0.2677447719897543 1102 30-31 0.3749119229286251 1102 32-33 0.2527240218977411 1102 34-35 0.22173884058375393 1102 36-37 0.17321812162568762 1102 38-39 0.2534841164796475 1102 40-41 0.34118820354294854 1102 42-43 0.2427035026693929 1102 44-45 0.28514186472651915 1102 46-47 0.3194455791711164 1102 48-49 0.43747118482004765 1102 50-51 0.4529061307071487 1102 52-53 0.4741329473508742 1102 54-55 0.4847691908115124 1102 56-57 0.4921175605646013 1102 58-59 0.27227075898472464 1102 60-61 0.3973752270655595 1102 62-63 0.1538731510988285 1102 64-65 0.3341039463867972 1102 66-67 0.2642397221827508 1102 68-69 0.31103902803491223 1102 70-71 0.51432940149801 1102 72-73 0.531452904584242 1102 74-75 0.5144575912583953 1102 76-77 0.3920319605298985 1102 78-79 0.4588250322331149 1102 80-81 0.7427617383750977 1102 82-83 0.7874574480880412 1102 84-85 0.7052812983142616 1102 86-87 0.7365916723612678 1102 88-89 0.6350903106925472 1102 90-91 0.7382861199195041 1102 92-93 0.6940160694633235 1102 94-95 0.6701264888937395 1102 96-97 0.6472761376324314 1102 98-99 0.8688677350906318 1102 100-101 0.027489967889732014 1103 1 0.17048604760612562 1103 2 0.15630927911833936 1103 3 0.16013683175909676 1103 4 0.05843664139136706 1103 5 0.20697493951482215 1103 6 0.1968099414118072 1103 7 0.22998082458936153 1103 8 0.10659598209966958 1103 9 0.18874030903676697 1103 10-11 0.1528160678193231 1103 12-13 0.1723901041934539 1103 14-15 0.04018223219529915 1103 16-17 0.19030785135664274 1103 18-19 0.34316341155864194 1103 20-21 0.39127123264560026 1103 22-23 0.30935141756535245 1103 24-25 0.2670699569384354 1103 26-27 0.21285595410774505 1103 28-29 0.3256885935828677 1103 30-31 0.4018926677296619 1103 32-33 0.42353210260580454 1103 34-35 0.4839375789508722 1103 36-37 0.40205765107285885 1103 38-39 0.46153026875681036 1103 40-41 0.3425580584185326 1103 42-43 0.40206955739824224 1103 44-45 0.45420730388703845 1103 46-47 0.5087558594690691 1103 48-49 0.7591746961437593 1103 50-51 0.7806846290848029 1103 52-53 0.6069073812151515 1103 54-55 0.6043129157672311 1103 56-57 0.5981429417426938 1103 58-59 0.46980709949688304 1103 60-61 0.39996592116167307 1103 62-63 0.41612882235742177 1103 64-65 0.597543326719304 1103 66-67 0.749305977216359 1103 68-69 0.62627545795646 1103 70-71 0.5757020683633627 1103 72-73 0.41817130262138846 1103 74-75 0.3646166568064686 1103 76-77 0.5279817645322318 1103 78-79 0.38267654729161293 1103 80-81 0.5531143163459298 1103 82-83 0.7139887695945184 1103 84-85 0.5379951357792727 1103 86-87 0.5884448627319863 1103 88-89 0.7703806832910942 1103 90-91 0.7331398815994099 1103 92-93 0.6687591536022168 1103 94-95 0.7041312672960736 1103 96-97 0.4992951759913389 1103 98-99 0.5084706504507324 1103 100-101 0.037797296588561125 1104 1 0.09819965902102012 1104 2 0.1441270613831449 1104 3 0.06832377892932584 1104 4 0.0893395394778338 1104 5 0.12200948101458664 1104 6 0.21607977417248492 1104 7 0.1585919452992286 1104 8 0.21762936925865972 1104 9 0.24822528252708764 1104 10-11 0.2601968443096254 1104 12-13 0.206736412030736 1104 14-15 0.20100216904277346 1104 16-17 0.2185644512253333 1104 18-19 0.3342051293970343 1104 20-21 0.3862023130842829 1104 22-23 0.36336401258132867 1104 24-25 0.29390884701549425 1104 26-27 0.3763310615333566 1104 28-29 0.5041346416934758 1104 30-31 0.4755439989105881 1104 32-33 0.31585760429108944 1104 34-35 0.2701328399897278 1104 36-37 0.2565238571861954 1104 38-39 0.3182337105788804 1104 40-41 0.3365323248377692 1104 42-43 0.38202876321925316 1104 44-45 0.62819989406524 1104 46-47 0.5348121139694584 1104 48-49 0.3292131087944341 1104 50-51 0.42170799514624946 1104 52-53 0.21171750803386402 1104 54-55 0.278280756729707 1104 56-57 0.3777740570170707 1104 58-59 0.3904270253239446 1104 60-61 0.4810709689743504 1104 62-63 0.5183421493898237 1104 64-65 0.5291042737643821 1104 66-67 0.5808389996746399 1104 68-69 0.5230819685878814 1104 70-71 0.5031084159301358 1104 72-73 0.30077316432277 1104 74-75 0.32942140417301147 1104 76-77 0.41278817429892456 1104 78-79 0.48435099517396907 1104 80-81 0.6391211097721659 1104 82-83 0.582429877201303 1104 84-85 0.27046265126621094 1104 86-87 0.42448411031847044 1104 88-89 0.6591530381369424 1104 90-91 0.7766737520685538 1104 92-93 0.7833540929946565 1104 94-95 0.8749993857299465 1104 96-97 0.6019661300315811 1104 98-99 0.6164472759838908 1104 100-101 0.31808123079860806 1105 1 0.02251375552054924 1105 2 0.022083541988635602 1105 3 0.02480438441655508 1105 4 0.02122222632741 1105 5 -0.01990158804879627 1105 6 0.018350351277497623 1105 7 -0.1280684142088191 1105 8 -0.052001661961774914 1105 9 0.0400890479007856 1105 10-11 0.03503128139571743 1105 12-13 -0.14236481786519306 1105 14-15 -0.027474652149287238 1105 16-17 0.03975650420546373 1105 18-19 0.09437542267991006 1105 20-21 0.10474535944189967 1105 22-23 0.07481150548577631 1105 24-25 0.012451893373111034 1105 26-27 0.016823019906105685 1105 28-29 0.07461713932828928 1105 30-31 0.12502365832402518 1105 32-33 -0.02378288766728076 1105 34-35 0.18404012475794218 1105 36-37 0.18083795368572453 1105 38-39 0.28039075882863784 1105 40-41 0.17711889058990238 1105 42-43 0.10293699406125967 1105 44-45 0.10975145508700024 1105 46-47 0.13320378852007053 1105 48-49 0.009440166504937508 1105 50-51 0.0025973045123777183 1105 52-53 0.16204863386168 1105 54-55 0.15623723733519768 1105 56-57 0.09631710337468036 1105 58-59 -0.019222927372361198 1105 60-61 -0.10644085694806904 1105 62-63 -0.10133618552029588 1105 64-65 0.057486487577058654 1105 66-67 -0.020863933154124936 1105 68-69 0.07416995345070632 1105 70-71 0.002162342136379891 1105 72-73 -0.08380583283900478 1105 74-75 0.27975253000082745 1105 76-77 -0.02051362324128192 1105 78-79 -0.20628287426784553 1105 80-81 -0.13713243800456 1105 82-83 -0.043870974265107776 1105 84-85 -0.029915778251289993 1105 86-87 0.20404891637334188 1105 88-89 0.06312654807071993 1105 90-91 0.16077971233345423 1105 92-93 0.16556790567204516 1105 94-95 0.16165974523798354 1105 96-97 -0.2215911074329462 1105 98-99 -0.004177132719988208 1105 100-101 -0.38816285624017866 1106 1 0.0812425763685738 1106 2 0.1718410030705968 1106 3 0.14999144900759376 1106 4 0.08080853405941468 1106 5 0.1395092659978161 1106 6 0.14699660568534512 1106 7 0.1368434289149505 1106 8 0.09664803271440547 1106 9 0.1540952403837963 1106 10-11 0.0973951757794822 1106 12-13 0.23339118110272494 1106 14-15 0.29669753328118276 1106 16-17 0.2163811709294663 1106 18-19 0.14841558781279218 1106 20-21 0.13132014227495858 1106 22-23 0.23869943874456823 1106 24-25 0.2695405662895993 1106 26-27 0.2208829665819465 1106 28-29 0.24290388569450982 1106 30-31 0.2266530554169961 1106 32-33 0.26189699536359257 1106 34-35 0.21821919072508678 1106 36-37 0.27821173884096595 1106 38-39 0.2773379507096152 1106 40-41 0.15507623126246983 1106 42-43 0.16790775178035489 1106 44-45 0.48807475430434266 1106 46-47 0.34787997326248643 1106 48-49 0.26987020004755635 1106 50-51 0.2752686728791147 1106 52-53 0.2342779277466036 1106 54-55 0.29985854979756965 1106 56-57 0.24155405185666012 1106 58-59 0.4378812267516565 1106 60-61 0.4769667288436281 1106 62-63 0.5383898831130551 1106 64-65 0.6107233500523321 1106 66-67 0.4254515394527445 1106 68-69 0.25274095198859214 1106 70-71 0.36680455422892777 1106 72-73 0.580220571220508 1106 74-75 0.40322161058911377 1106 76-77 0.32571756279122965 1106 78-79 0.5710831702836288 1106 80-81 0.6465047856988875 1106 82-83 0.49631265005758607 1106 84-85 0.6038624964541377 1106 86-87 0.630569514980035 1106 88-89 0.34566202583798855 1106 90-91 0.4780330880967618 1106 92-93 0.43800576582513884 1106 94-95 0.21521311240062246 1106 96-97 0.11073150373072593 1106 98-99 0.35600004109956984 1106 100-101 -0.24810910204735137 1107 1 0.12185402897291198 1107 2 0.04062456825985805 1107 3 0.09826061756145776 1107 4 -0.09825325824081688 1107 5 0.03978102271085504 1107 6 0.07488078556240652 1107 7 0.11377969549534583 1107 8 0.0950951372113309 1107 9 0.12784160078496143 1107 10-11 0.13076948829969837 1107 12-13 0.029389239983345306 1107 14-15 0.1020356115729939 1107 16-17 0.13022944009802018 1107 18-19 0.12929556193119396 1107 20-21 0.08663233897505762 1107 22-23 0.1878016710318562 1107 24-25 0.08485599818866518 1107 26-27 0.13855847612578742 1107 28-29 0.2354677808740533 1107 30-31 0.1655369117741614 1107 32-33 0.28820725094431054 1107 34-35 0.10989664138830335 1107 36-37 0.0968966304617993 1107 38-39 0.022714851388997204 1107 40-41 0.05658706917900247 1107 42-43 0.08579193165742538 1107 44-45 0.030578798303046995 1107 46-47 -0.09386865177796011 1107 48-49 -0.14885674915982605 1107 50-51 -0.3536701818604513 1107 52-53 -0.23813553068108462 1107 54-55 -0.2745769079830822 1107 56-57 -0.3728862587224384 1107 58-59 -0.3933627072502759 1107 60-61 -0.2852429120492914 1107 62-63 -0.29188672639843105 1107 64-65 -0.2954979375568527 1107 66-67 -0.20115958963169334 1107 68-69 -0.06474044561736036 1107 70-71 -0.11068978417288733 1107 72-73 -0.17938958730423948 1107 74-75 -0.30831187372017155 1107 76-77 -0.2555393120065723 1107 78-79 -0.16904300247599835 1107 80-81 -0.15341433432699247 1107 82-83 -0.3483476540511141 1107 84-85 -0.30940020170180205 1107 86-87 -0.27095588133184023 1107 88-89 -0.3802988282545421 1107 90-91 -0.04093811863245023 1107 92-93 0.15287797546603343 1107 94-95 0.06471327416057093 1107 96-97 0.26599840764531635 1107 98-99 0.26141414656705564 1107 100-101 -0.47917995290467985 1108 1 -0.06290731953704665 1108 2 -0.06941198482360988 1108 3 -0.032277808452505496 1108 4 -0.061613395156406625 1108 5 -0.10022317289492833 1108 6 -0.05665749254890784 1108 7 -0.06533946876413665 1108 8 -0.017427840521769156 1108 9 3.555534380055292E-4 1108 10-11 0.06659596227034115 1108 12-13 0.02790078091766901 1108 14-15 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sequence quality scores pass #Quality Count 3 1.0 4 12.0 5 28.0 6 77.0 7 187.0 8 352.0 9 703.0 10 1383.0 11 2347.0 12 3887.0 13 5571.0 14 6473.0 15 9125.0 16 12942.0 17 19872.0 18 19107.0 19 10256.0 20 7556.0 21 7589.0 22 8136.0 23 8387.0 24 9425.0 25 10707.0 26 12030.0 27 13826.0 28 16333.0 29 20209.0 30 24192.0 31 28750.0 32 36425.0 33 47163.0 34 65514.0 35 98465.0 36 158687.0 37 292012.0 38 224595.0 39 11379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57496783985637 22.658240947413855 16.090840942534093 19.675950270195685 2 14.188453911902709 24.074748911580183 40.44088018543976 21.295916991077345 3 18.85552771501789 28.594298581808037 32.742063980738926 19.808109722435145 4 11.010862836065588 19.902438043633968 41.55196057980922 27.534738540491226 5 10.32333838484112 40.80964863119218 35.99270505309947 12.874307930867227 6 24.93777765491081 41.681724851156446 18.570113336399423 14.810384157533324 7 23.441760638952903 37.032243363717775 23.325986447215094 16.200009550114224 8 23.45683976667563 34.70570150196489 21.455085561483887 20.38237316987559 9 24.62153483739255 18.94742662119472 24.355472006018246 32.075566535394486 10-11 22.34998990536172 28.727874521551843 28.920803583470928 20.001331989615505 12-13 25.02050342505632 27.750244407528506 28.719664774236136 18.509587393179043 14-15 21.7121389568851 28.762605826953973 29.849651585749783 19.675603630411143 16-17 17.699016812044892 31.75016001099133 30.642942593753038 19.90788058321074 18-19 17.352850750982444 30.835056961404973 33.908099418364536 17.903992869248047 20-21 19.358458511036662 28.5096041477654 33.468626618178895 18.66331072301904 22-23 18.575642349897755 28.84415135088041 33.517926988539024 19.062279310682808 24-25 19.169399062747654 29.35908627111289 33.5932669962855 17.87824766985395 26-27 18.701470061554506 28.41170938899978 35.39997863708811 17.486841912357605 28-29 17.335593527033105 30.208184112798577 34.53023909632463 17.925983263843687 30-31 18.420452993751375 28.86496892443095 34.07560339548447 18.638974686333203 32-33 18.321600934235736 29.42930527945393 34.09277684650202 18.156316939808313 34-35 19.19327791641549 28.833651639194898 33.95216908094576 18.020901363443855 36-37 18.909946073812385 28.19274160469687 34.650895913785135 18.24641640770561 38-39 18.000315884684497 29.84754165048491 34.03623959811424 18.115902866716354 40-41 18.10965997941503 29.79550339543034 34.04554078265601 18.049295842498616 42-43 17.793825523148215 29.948868582286565 32.71711164041954 19.540194254145682 44-45 18.650664828055355 29.04169506335376 32.78360698175226 19.524033126838628 46-47 19.575919925773423 30.017404708696283 32.12373051395204 18.282944851578257 48-49 18.885502476000266 29.8739422230302 32.16173965931644 19.0788156416531 50-51 17.615136194756616 30.360854322083952 32.777216960781445 19.246792522377994 52-53 18.4840785354481 31.479605898619674 30.94500893438318 19.091306631549052 54-55 18.281389926975137 30.06635653927317 31.356878553543048 20.295374980208646 56-57 19.298962093957325 30.144680223657254 30.74579365109284 19.81056403129258 58-59 19.81705414277534 29.829314480812176 30.895381660092497 19.45824971631999 60-61 18.9978746273058 30.619817990354143 31.667382943234873 18.714924439105182 62-63 18.240471615801006 30.38565080274629 31.82661065516948 19.547266926283225 64-65 18.132596333318812 31.291741087136455 31.01038144609724 19.565281133447492 66-67 18.573581573651946 30.283861195862883 30.18182396986802 20.960733260617147 68-69 18.14325807397478 30.200980186821937 30.75997989360365 20.895781845599632 70-71 19.2699251361 30.147369585058886 30.621986521733486 19.96071875710763 72-73 18.96585666618916 29.125712174636405 31.710777303902226 20.197653855272208 74-75 17.971890830466204 31.52354480134506 30.060283001718187 20.444281366470555 76-77 18.21937662962747 29.99135057990526 30.622972071554976 21.166300718912296 78-79 18.26756994189819 30.060615404079655 30.742721983127897 20.92909267089426 80-81 18.78837863513217 29.8392055612056 30.19838351073722 21.17403229292501 82-83 18.330815956732955 29.850766899304098 31.017011769259188 20.80140537470376 84-85 18.009504876841223 30.007631714086337 30.484802333578788 21.498061075493652 86-87 17.741477624137847 30.277260512002968 30.428086406423162 21.553175457436026 88-89 18.06441979073558 29.908701824491295 29.758998895841 22.26787948893212 90-91 18.17231932273288 28.978105168048547 30.433568233796137 22.41600727542244 92-93 18.771964215554455 29.880631949488272 30.2245198344982 21.122884000459074 94-95 18.284162092313622 29.04433893704671 30.423870269183485 22.247628701456186 96-97 17.512103094320782 29.177735165279806 30.81214506455961 22.498016675839803 98-99 17.930670214178516 29.69513709678433 29.357615247710793 23.016577441326362 100-101 17.810024893220525 28.49350149975057 30.535097430381292 23.16137617664762 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2441.0 1 1581.5 2 867.5 3 1002.0 4 862.5 5 874.0 6 1426.5 7 2665.0 8 4112.0 9 4522.0 10 3643.0 11 2846.0 12 2744.5 13 2802.5 14 2911.0 15 3025.0 16 3137.5 17 3334.5 18 3625.0 19 4011.0 20 4492.5 21 5200.5 22 5898.0 23 6662.5 24 7680.5 25 9418.0 26 13013.5 27 16867.5 28 17904.0 29 19145.5 30 23006.0 31 26799.5 32 31615.0 33 37751.5 34 44088.0 35 48444.0 36 52743.5 37 57232.0 38 55055.5 39 50369.0 40 47146.0 41 44408.0 42 41384.0 43 39588.0 44 39480.0 45 38485.0 46 37317.0 47 44598.5 48 63510.5 49 60022.5 50 37404.5 51 24570.0 52 17771.0 53 14178.5 54 11725.5 55 9917.0 56 8275.5 57 6687.0 58 5558.5 59 4616.0 60 3520.0 61 2599.5 62 1841.5 63 1285.5 64 874.5 65 595.0 66 412.0 67 276.0 68 197.0 69 162.0 70 131.5 71 101.0 72 84.5 73 85.0 74 89.5 75 71.5 76 43.5 77 24.0 78 17.5 79 14.0 80 15.0 81 10.5 82 5.0 83 2.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42891741077973333 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.5395638613625E-4 16-17 0.0029320526127520835 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.377293179291667E-5 26-27 0.003811668396577708 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012105188644076456 36-37 0.026179041185286456 38-39 0.019225887846474374 40-41 0.009508227758496041 42-43 0.009382568360806667 44-45 0.004062987191956458 46-47 0.005068262373471458 48-49 0.0036860089988883333 50-51 6.701834543433333E-4 52-53 0.0 54-55 0.0 56-57 1.25659397689375E-4 58-59 0.001382253374583125 60-61 0.0025131879537875 62-63 0.0016335721699618749 64-65 0.0029320526127520835 66-67 0.0017592315676512498 68-69 0.004439965385024583 70-71 2.9320526127520833E-4 72-73 0.0 74-75 0.0 76-77 0.0952917099144427 78-79 0.01629383523372229 80-81 4.1886465896458333E-4 82-83 0.0 84-85 0.0 86-87 0.10626596397931479 88-89 0.42016313940737354 90-91 0.42250878149757515 92-93 0.0 94-95 4.188646589645833E-5 96-97 0.0 98-99 2.0943232948229167E-4 100-101 3.76978193068125E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1193703.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.49653729376483 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15425783872499 25.235685837312648 2 13.496456107076723 9.311620028628646 3 5.046134476898033 5.222224985149965 4 2.4120998107105738 3.328363643058416 5 1.3395677558124055 2.310522452295375 6 0.8421958104758873 1.7431703510840366 7 0.5812791453870807 1.4036482401853223 8 0.3970788014077225 1.095827494505995 9 0.29863673646101574 0.9271739984954083 >10 1.8814097993411014 12.995000036420882 >50 0.29926875063908803 7.2976221239083205 >100 0.22681812974858465 15.14133982405105 >500 0.015072587388679684 3.3867329862425963 >1k 0.008265612438953376 5.85809873435034 >5k 9.724249928180442E-4 2.5138005463208657 >10k+ 4.862124964090221E-4 2.229168717990156 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14276 1.1959423742756783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12305 1.0308259257118397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9614 0.8053929662571008 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7954 0.6663298994808591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6418 0.5376546762469392 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5989 0.5017160885077779 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4422 0.3704439043882775 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4004 0.3354268188988383 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3957 0.33148949110457127 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3745 0.3137296295644729 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3599 0.30149878152270704 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3376 0.28281741773288666 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3335 0.2793827275293771 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3078 0.25785308405859747 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2888 0.24193622701794332 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2498 0.20926478361870585 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.17433147106105956 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2064 0.17290733122058 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 0.16352476285977333 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1939 0.1624357147464654 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1856 0.15548256140765332 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1840 0.15414219449896666 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1776 0.14878072686422 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1719 0.14400566975202375 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1668 0.139733250230585 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1377 0.11535532707884624 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1374 0.1151040082834675 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1371 0.11485268948808874 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1358 0.11376364137478084 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1358 0.11376364137478084 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1299 0.10882103839899875 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1266 0.1060565316498325 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1214 0.10170033919660083 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6754586358583333E-4 0.0 16-17 0.0 0.0 0.0 2.9320526127520833E-4 0.0 18-19 0.0 0.0 0.0 5.864105225504167E-4 0.0 20-21 0.0 0.0 0.0 0.0010471616474114582 0.0 22-23 0.0 0.0 0.0 0.0018430044994441667 0.0 24-25 0.0 0.0 0.0 0.003644122532991875 0.0 26-27 0.0 0.0 0.0 0.006618061611640417 0.0 28-29 0.0 0.0 0.0 0.018262499130855835 0.0 30-31 0.0 0.0 0.0 0.04816943578092708 0.0 32-33 0.0 0.0 0.0 0.10396220835500958 0.0 34-35 0.0 0.0 0.0 0.17860389058249831 0.0 36-37 0.0 0.0 0.0 0.29035698159424916 0.0 38-39 0.0 0.0 0.0 0.447808206899036 0.0 40-41 0.0 0.0 0.0 0.6267053027428096 0.0 42-43 0.0 0.0 0.0 0.8061050361773405 0.0 44-45 0.0 0.0 0.0 1.0172965972272836 0.0 46-47 0.0 0.0 0.0 1.2764062752627747 0.0 48-49 0.0 0.0 0.0 1.5332540841398572 0.0 50-51 0.0 0.0 0.0 1.7977671162759914 0.0 52-53 0.0 0.0 0.0 2.055075676277935 0.0 54-55 0.0 0.0 0.0 2.3401549631692307 0.0 56-57 0.0 0.0 0.0 2.69815858718626 0.0 58-59 0.0 0.0 0.0 3.0645813908484776 0.0 60-61 0.0 0.0 0.0 3.4639269567053113 0.0 62-63 0.0 0.0 0.0 3.874079230763431 0.0 64-65 0.0 0.0 0.0 4.292818230330325 0.0 66-67 0.0 8.377293179291667E-5 0.0 4.778240483604381 0.0 68-69 0.0 8.377293179291667E-5 0.0 5.3396447860146115 0.0 70-71 0.0 8.377293179291667E-5 0.0 5.941595187412615 0.0 72-73 0.0 8.377293179291667E-5 0.0 6.50873793565066 0.0 74-75 0.0 8.377293179291667E-5 0.0 7.135652670722952 0.0 76-77 0.0 8.377293179291667E-5 0.0 7.781709520709926 0.0 78-79 0.0 8.377293179291667E-5 0.0 8.563143428474252 0.0 80-81 0.0 8.377293179291667E-5 0.0 9.388181147236793 0.0 82-83 0.0 8.377293179291667E-5 0.0 10.174306339181522 0.0 84-85 0.0 8.377293179291667E-5 0.0 10.970777488202678 0.0 86-87 0.0 8.377293179291667E-5 0.0 11.806831347495985 0.0 88-89 0.0 8.377293179291667E-5 0.0 12.709359028166972 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAGAT 35 2.742977E-4 54.464893 1 GTAAGCG 245 0.0 39.705715 94-95 ATGGGCG 130 3.0633782E-7 29.20195 5 GTACAGG 360 0.0 27.799788 1 CATTCCG 380 0.0 27.472889 9 GATTGGC 450 0.0 27.417389 9 GCAACGG 35 0.008432106 27.117231 60-61 GTGCGAA 35 0.008432106 27.117231 12-13 CTCCGAC 315 0.0 25.609648 94-95 GAGTACT 8545 0.0 23.29718 12-13 TCAATAC 265 3.274181E-11 23.278915 3 AGTACTT 8805 0.0 22.690088 12-13 ATATGAG 695 0.0 22.531721 3 GTACATG 7430 0.0 22.321077 1 TGCGAAG 65 2.9591314E-4 21.905132 42-43 GTACTTT 9025 0.0 21.900358 14-15 CCCTATA 220 5.6988938E-8 21.569622 2 TACATGG 7680 0.0 21.255066 2 CATGGGT 670 0.0 21.247688 4 GGATATG 565 0.0 21.08707 1 >>END_MODULE