##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939183_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50496 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03841888466413 33.0 31.0 34.0 30.0 34.0 2 32.19290636882129 33.0 31.0 34.0 30.0 34.0 3 32.212690114068444 34.0 31.0 34.0 30.0 34.0 4 35.82782794676806 37.0 35.0 37.0 35.0 37.0 5 35.78263624841571 37.0 35.0 37.0 35.0 37.0 6 35.92496435361217 37.0 35.0 37.0 35.0 37.0 7 35.83394724334601 37.0 35.0 37.0 35.0 37.0 8 35.87632683776933 37.0 35.0 37.0 35.0 37.0 9 37.43542062737642 39.0 37.0 39.0 34.0 39.0 10-11 37.416577550697085 39.0 37.0 39.0 34.0 39.0 12-13 37.34509664131812 39.0 37.0 39.0 34.0 39.0 14-15 38.30309331432193 40.0 37.5 41.0 33.0 41.0 16-17 38.30349928707224 40.0 37.5 41.0 33.5 41.0 18-19 38.4682846166033 40.0 38.0 41.0 34.0 41.0 20-21 38.579075570342205 40.0 38.0 41.0 34.0 41.0 22-23 38.48667221166033 40.0 38.0 41.0 34.0 41.0 24-25 38.31119296577947 40.0 37.0 41.0 34.0 41.0 26-27 37.93640090304183 40.0 36.5 41.0 33.0 41.0 28-29 37.49747504752852 39.5 36.0 41.0 33.0 41.0 30-31 37.012010852344744 39.0 35.0 41.0 32.5 41.0 32-33 36.32140169518378 38.0 35.0 41.0 31.0 41.0 34-35 35.887020754119135 38.0 35.0 41.0 30.5 41.0 36-37 35.46258119455006 38.0 35.0 40.5 30.0 41.0 38-39 34.95790755703422 37.5 35.0 40.0 27.0 41.0 40-41 34.55263783269962 37.0 35.0 40.0 25.0 41.0 42-43 33.996900744613434 37.0 34.5 40.0 22.5 41.0 44-45 33.20283784854246 37.0 33.5 40.0 10.0 41.0 46-47 32.159982968948036 36.0 33.0 40.0 2.0 41.0 48-49 31.187024714828897 35.0 32.0 40.0 2.0 41.0 50-51 30.402616048795945 35.0 30.5 40.0 2.0 41.0 52-53 29.899645516476554 35.0 29.5 40.0 2.0 41.0 54-55 29.364494217363752 35.0 27.5 40.0 2.0 41.0 56-57 28.61875198035488 35.0 24.5 39.5 2.0 41.0 58-59 27.85898883079848 35.0 19.5 39.0 2.0 41.0 60-61 26.991088403041825 35.0 4.5 39.0 2.0 40.5 62-63 26.301835788973385 35.0 2.0 38.0 2.0 40.0 64-65 25.51455560836502 34.0 2.0 37.5 2.0 40.0 66-67 24.742583570975917 34.0 2.0 37.0 2.0 39.0 68-69 24.02671498732573 33.0 2.0 36.0 2.0 39.0 70-71 23.44159933460076 33.0 2.0 35.5 2.0 38.0 72-73 22.929083491761723 32.5 2.0 35.0 2.0 37.0 74-75 22.51390209125475 32.0 2.0 35.0 2.0 37.0 76-77 21.00430727186312 29.5 2.0 33.5 2.0 35.0 78-79 21.68548993979721 31.0 2.0 35.0 2.0 35.5 80-81 21.655348938529784 31.0 2.0 35.0 2.0 35.0 82-83 21.37559410646388 31.0 2.0 35.0 2.0 35.0 84-85 21.07490692332066 31.0 2.0 35.0 2.0 35.0 86-87 20.669320342205324 30.0 2.0 35.0 2.0 35.0 88-89 20.220492712294046 29.0 2.0 34.0 2.0 35.0 90-91 19.79965739860583 29.0 2.0 34.0 2.0 35.0 92-93 19.517021150190114 29.0 2.0 34.0 2.0 35.0 94-95 19.215017031051964 27.0 2.0 34.0 2.0 35.0 96-97 18.743761882129277 27.0 2.0 34.0 2.0 35.0 98-99 18.166379515209126 24.5 2.0 34.0 2.0 35.0 100-101 15.777012040557668 10.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2294956295148438 1101 2 0.04042639775725121 1101 3 -0.0033198275243648823 1101 4 -0.14846103701604818 1101 5 -0.18575838405806167 1101 6 -0.24620201820258103 1101 7 -0.19642664742190874 1101 8 0.10080691790740559 1101 9 0.11372081400695322 1101 10-11 0.05614923005926897 1101 12-13 -0.6115470530116554 1101 14-15 -0.6244376628137331 1101 16-17 -0.9370384192367283 1101 18-19 -0.2910183599747782 1101 20-21 0.3925822136187591 1101 22-23 -0.1420750819887715 1101 24-25 -0.3212258030644648 1101 26-27 -0.00997760804605008 1101 28-29 -0.9046598146818567 1101 30-31 -0.10107479770287142 1101 32-33 -1.3528042501895357 1101 34-35 -0.7160235689864152 1101 36-37 -1.4931200591028428 1101 38-39 -1.6087587822925826 1101 40-41 -1.4441823092315147 1101 42-43 -3.2048303688656787 1101 44-45 -2.884053705121339 1101 46-47 -2.1300404222194302 1101 48-49 -3.2262919039165716 1101 50-51 -4.058465604374515 1101 52-53 -4.745560248351033 1101 54-55 -6.313255164881014 1101 56-57 -6.053479398415476 1101 58-59 -7.257175106068605 1101 60-61 -6.41778206204442 1101 62-63 -5.864931340523274 1101 64-65 -5.923436016577707 1101 66-67 -5.056149872710218 1101 68-69 -5.0462682305395035 1101 70-71 -4.698603804129839 1101 72-73 -3.8071109053269794 1101 74-75 -3.852667571503691 1101 76-77 -4.095767862768039 1101 78-79 -4.9858010091104745 1101 80-81 -4.55121971599144 1101 82-83 -4.196041327131834 1101 84-85 -3.7506128228198534 1101 86-87 -3.329914817021116 1101 88-89 -4.0029744271118695 1101 90-91 -3.0204620037689835 1101 92-93 -3.3648755523942278 1101 94-95 -3.7034162453184507 1101 96-97 -3.4975887331438464 1101 98-99 -4.58433198398226 1101 100-101 -2.9356695691123935 1102 1 0.19813674449741825 1102 2 -0.013580570883860332 1102 3 0.5129403350772606 1102 4 -0.4272066816153526 1102 5 0.10866670304995552 1102 6 -0.476167174997002 1102 7 0.07418891587658294 1102 8 -0.11347879637830971 1102 9 -0.5181026470848025 1102 10-11 -0.6709123262705745 1102 12-13 -0.2610824769373252 1102 14-15 -0.9275735513154757 1102 16-17 -0.6620674552413703 1102 18-19 -0.36796377228604626 1102 20-21 -0.037441015184960236 1102 22-23 0.31088659048509726 1102 24-25 0.24846060808536663 1102 26-27 0.7403127520004134 1102 28-29 -1.026901394240511 1102 30-31 -0.388240883649452 1102 32-33 0.39545358953171217 1102 34-35 0.44396481661172515 1102 36-37 0.9342899292827553 1102 38-39 0.8822400562672357 1102 40-41 0.7799756466337584 1102 42-43 1.7039965765466363 1102 44-45 2.0253539603838817 1102 46-47 1.0212371619849847 1102 48-49 1.808551301658337 1102 50-51 2.301871213279373 1102 52-53 2.0831273242389727 1102 54-55 1.374898145223515 1102 56-57 1.0498887781234352 1102 58-59 1.6191315141404523 1102 60-61 1.5891865790705637 1102 62-63 1.1330361391515211 1102 64-65 0.47580904730149953 1102 66-67 0.24756673588444045 1102 68-69 1.6328839181248398 1102 70-71 2.1660884142209156 1102 72-73 1.4759901399691877 1102 74-75 2.1786913135137347 1102 76-77 1.9530126250368376 1102 78-79 1.9956159479162388 1102 80-81 1.3851914338343434 1102 82-83 0.49966134418059127 1102 84-85 1.7058331702115055 1102 86-87 1.7685172387280126 1102 88-89 0.9685702883352825 1102 90-91 1.626750539785025 1102 92-93 2.128155806490792 1102 94-95 1.1017521635084968 1102 96-97 2.076453078702844 1102 98-99 2.4946459486542096 1102 100-101 1.339882114975877 1103 1 0.07908912544979785 1103 2 0.08165752435423457 1103 3 0.03674985888677895 1103 4 -0.3795876339963087 1103 5 0.03723813162137901 1103 6 -0.1904528892827173 1103 7 0.19323653492420334 1103 8 0.3627116798121648 1103 9 0.45808782910567203 1103 10-11 0.2695638642056153 1103 12-13 0.11986990401505437 1103 14-15 0.1200454963035682 1103 16-17 0.27840873523481946 1103 18-19 0.0010838467615741365 1103 20-21 0.27208279433884996 1103 22-23 0.28707706667557176 1103 24-25 0.7484606080853595 1103 26-27 -0.09302058133292235 1103 28-29 0.08024146290235024 1103 30-31 0.31414006873150413 1103 32-33 0.4906916847698142 1103 34-35 -0.008416135769223843 1103 36-37 0.7438137388065726 1103 38-39 0.9417638657910459 1103 40-41 0.5180708847289992 1103 42-43 0.08494895749901588 1103 44-45 -0.5103603253303959 1103 46-47 -1.6930485523007306 1103 48-49 -0.9771629840559477 1103 50-51 -1.0790811676730065 1103 52-53 -0.9287774376657865 1103 54-55 -1.053673283347912 1103 56-57 -0.5453493171146597 1103 58-59 -0.9880113430024018 1103 60-61 -0.494146754262772 1103 62-63 -0.9264876703722855 1103 64-65 -0.11942904793659537 1103 66-67 -0.0857665974488917 1103 68-69 0.3828839181248398 1103 70-71 -0.5124830143505115 1103 72-73 -0.5954384314593852 1103 74-75 0.15488178970420918 1103 76-77 0.2387269107511223 1103 78-79 0.4241873764876658 1103 80-81 0.34947714812005515 1103 82-83 0.05918515370440147 1103 84-85 0.6582141225924616 1103 86-87 0.8637553339661075 1103 88-89 1.3733321930971876 1103 90-91 1.555321968356452 1103 92-93 1.4733939017288868 1103 94-95 1.5660378777942086 1103 96-97 1.1597864120361798 1103 98-99 1.6136935677018265 1103 100-101 2.423215448309209 1104 1 -0.18281563645496135 1104 2 -0.18024723755052463 1104 3 -0.510869188732265 1104 4 -0.16530191971059338 1104 5 0.20390479828805042 1104 6 0.3333566345268082 1104 7 0.12180796349562684 1104 8 -0.3515740344735434 1104 9 -0.08953121851337187 1104 10-11 -0.11138851674676431 1104 12-13 -0.011082476937325225 1104 14-15 0.012902639160714102 1104 16-17 -0.6739722171461366 1104 18-19 0.036798132475858836 1104 20-21 0.23636850862456527 1104 22-23 0.08469611429461565 1104 24-25 -0.07296796334320987 1104 26-27 -0.7477824860948274 1104 28-29 -0.49118710852621916 1104 30-31 -0.37633612174468567 1104 32-33 -0.15216545808733173 1104 34-35 -0.913178040531136 1104 36-37 -4.02999578500296 1104 38-39 -3.0939504199232424 1104 40-41 -3.600976734318621 1104 42-43 -5.11743199488194 1104 44-45 -5.474646039616111 1104 46-47 -5.824000933253114 1104 48-49 -5.453353460246422 1104 50-51 -5.198128786720627 1104 52-53 -4.440682199570546 1104 54-55 -4.375101854776485 1104 56-57 -3.8548731266384735 1104 58-59 -3.9046780096690696 1104 60-61 -4.041765801881819 1104 62-63 -4.986011479896096 1104 64-65 -4.90514333365088 1104 66-67 -4.7762427879250815 1104 68-69 -4.67663989139897 1104 70-71 -4.869625871493369 1104 72-73 -4.702581288602239 1104 74-75 -4.154642019819601 1104 76-77 -3.630320708296498 1104 78-79 -4.337717385417093 1104 80-81 -4.257665709022799 1104 82-83 -3.833671989152741 1104 84-85 -3.4846430202646843 1104 86-87 -4.1362446660338925 1104 88-89 -3.507620187855192 1104 90-91 -3.5518208887864056 1104 92-93 -2.967082288747303 1104 94-95 -4.219676407920076 1104 96-97 -4.2687850165352526 1104 98-99 -4.219639765631506 1104 100-101 -2.6958321707384094 1105 1 -0.32162009440835604 1105 2 0.2614954169580841 1105 3 0.3179048740843484 1105 4 0.25060486853661956 1105 5 -0.37309621488317646 1105 6 0.3505805453677411 1105 7 0.16942701111467784 1105 8 0.036977130673363945 1105 9 -0.03988582844245059 1105 10-11 0.14722343867369858 1105 12-13 0.20370982296135764 1105 14-15 -0.03699604371669807 1105 16-17 -0.2818749527084492 1105 18-19 0.7751466633775763 1105 20-21 0.5337342634371325 1105 22-23 0.5844428214881319 1105 24-25 0.499220486504818 1105 26-27 0.7362600670966586 1105 28-29 0.9186406523653687 1105 30-31 0.7634815074346477 1105 32-33 0.4357271457627121 1105 34-35 0.9715737325185145 1105 36-37 0.45809945309228084 1105 38-39 0.9280860542408931 1105 40-41 0.41726034774824683 1105 42-43 0.8063268703662843 1105 44-45 -0.7953147326252292 1105 46-47 -0.7551052898893857 1105 48-49 -0.5075581208340623 1105 50-51 -0.2753830926983376 1105 52-53 -0.664086454889695 1105 54-55 -1.3895395447460892 1105 56-57 -0.9566968348451574 1105 58-59 -0.7820842913306727 1105 60-61 -1.391056582023662 1105 62-63 -1.2056163431179812 1105 64-65 -0.6338667379061995 1105 66-67 -0.800052311734607 1105 68-69 -0.37294181642430146 1105 70-71 -0.08365829297259708 1105 72-73 0.16823938009076755 1105 74-75 0.6133417694407868 1105 76-77 0.218970071845348 1105 78-79 0.5954133136710524 1105 80-81 0.4908145341382948 1105 82-83 0.4596410807560751 1105 84-85 0.31373590577381805 1105 86-87 0.8680613116763389 1105 88-89 0.42956319613670146 1105 90-91 0.67310312337165 1105 92-93 0.5445691803509725 1105 94-95 0.6562101169026171 1105 96-97 -0.632513486646701 1105 98-99 -2.182152430271831 1105 100-101 -0.9476052203837977 1106 1 -0.02507754121686645 1106 2 -0.061199618502911335 1106 3 0.03377366841058915 1106 4 0.10255522314655252 1106 5 0.1592619411451892 1106 6 0.3660947297649031 1106 7 -0.39307298888532216 1106 8 0.07997358457406989 1106 9 0.01463544815329243 1106 10-11 0.2903971975389439 1106 12-13 -0.08995152455637623 1106 14-15 0.0501050201130937 1106 16-17 -0.19034126476518765 1106 18-19 -0.41855901038128707 1106 20-21 0.39410660386266017 1106 22-23 0.007315161913666657 1106 24-25 -0.11314653477177927 1106 26-27 0.38763418057183685 1106 28-29 0.16952717718805843 1106 30-31 0.15044959254102963 1106 32-33 1.0115250181031428 1106 34-35 0.47223862613553536 1106 36-37 1.0101827864256165 1106 38-39 1.0340257705529439 1106 40-41 1.3350351704432768 1106 42-43 0.9242346717847241 1106 44-45 0.9777349127648449 1106 46-47 1.296534781032605 1106 48-49 2.976706063563096 1106 50-51 2.554847403755563 1106 52-53 2.76765113376279 1106 54-55 3.516267192842559 1106 56-57 3.1942340162186724 1106 58-59 1.9643696093785508 1106 60-61 1.926984198118184 1106 62-63 1.5288694724848533 1106 64-65 1.803189999682452 1106 66-67 1.5020310215987287 1106 68-69 1.988538680029599 1106 70-71 1.8372193666018681 1106 72-73 1.9164663304453775 1106 74-75 2.2977389325613515 1106 76-77 2.7550959583701733 1106 78-79 0.8378778526781403 1106 80-81 1.5905485766914857 1106 82-83 0.6068042013234489 1106 84-85 0.9513688844972208 1106 86-87 1.3979815244422973 1106 88-89 1.7810702883352825 1106 90-91 1.891631492165974 1106 92-93 1.3945248541098394 1106 94-95 1.363656925413256 1106 96-97 1.4246673644171288 1106 98-99 1.2327411867494469 1106 100-101 1.1479178292615888 1107 1 0.07568776490558093 1107 2 0.09866432707531914 1107 3 0.29185189970311143 1107 4 0.26667086940505413 1107 5 0.22091160100913498 1107 6 0.27893486581932336 1107 7 0.0061617049922304545 1107 8 0.039582428111486934 1107 9 0.3322374889696178 1107 10-11 0.10119651726683543 1107 12-13 0.026332489049075036 1107 14-15 0.31732440786819893 1107 16-17 0.44847676244570067 1107 18-19 0.36502942499286206 1107 20-21 0.290790277332043 1107 22-23 0.2700702639544801 1107 24-25 0.13621571012618006 1107 26-27 0.2981358812521151 1107 28-29 0.2162958846710552 1107 30-31 0.5318271435614363 1107 32-33 1.5740250181031428 1107 34-35 1.7075702587885928 1107 36-37 1.0091198612555488 1107 38-39 1.0131924372196153 1107 40-41 0.5214722452732161 1107 42-43 1.0883503180432328 1107 44-45 1.7192315114042955 1107 46-47 2.5195364817128763 1107 48-49 3.0534492608420116 1107 50-51 2.3613950228031833 1107 52-53 2.120542290225373 1107 54-55 2.823877737060254 1107 56-57 1.757371771320713 1107 58-59 1.1701519223037202 1107 60-61 1.0687784158052551 1107 62-63 2.2231721935732907 1107 64-65 2.487713809206266 1107 66-67 2.2101517698980437 1107 68-69 1.620979156220077 1107 70-71 1.3633673257855428 1107 72-73 1.3076227930304114 1107 74-75 0.8113443747382227 1107 76-77 0.708114665853163 1107 78-79 1.8969764921339234 1107 80-81 1.767844495058835 1107 82-83 0.9928586230921574 1107 84-85 0.7874658232727327 1107 86-87 0.6511702999525006 1107 88-89 -0.4327902558824057 1107 90-91 0.5400158459074724 1107 92-93 0.3832578473071173 1107 94-95 0.8075344764336627 1107 96-97 1.2754326705395762 1107 98-99 2.447026901035162 1107 100-101 1.157909325860233 1108 1 -0.2172071708464962 1108 2 0.06843001112672198 1108 3 0.4018292239661463 1108 4 -0.05948181389048557 1108 5 -0.22731213292887986 1108 6 0.37303917420934596 1108 7 0.4472047888924564 1108 8 0.3971032142037032 1108 9 -0.42286455184670757 1108 10-11 0.12141571605746293 1108 12-13 0.10796514211029518 1108 14-15 0.3475587238167961 1108 16-17 0.4728531796792623 1108 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2316 82-83 1.3068042013234482 2316 84-85 0.8701188844972201 2316 86-87 -0.5791018088910356 2316 88-89 -1.0814297116647147 2316 90-91 -1.0208685078340238 2316 92-93 -0.890891812556827 2316 94-95 -0.25300974125341114 2316 96-97 -1.2544993022495383 2316 98-99 -1.150592146583886 2316 100-101 -0.42083217073840906 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 4.0 6 13.0 7 40.0 8 70.0 9 129.0 10 220.0 11 338.0 12 545.0 13 701.0 14 827.0 15 1076.0 16 1476.0 17 1947.0 18 1873.0 19 1327.0 20 1177.0 21 1362.0 22 1331.0 23 1173.0 24 1150.0 25 1065.0 26 852.0 27 763.0 28 805.0 29 994.0 30 1084.0 31 1326.0 32 1466.0 33 1880.0 34 2492.0 35 4161.0 36 5330.0 37 7232.0 38 4005.0 39 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.63710414431597 20.773234791592166 15.120991774943379 15.468669289148487 2 15.40914131812421 24.77621989860583 36.169201520912544 23.64543726235741 3 23.76425855513308 31.87183143219265 30.62816856780735 13.73574144486692 4 11.438529784537389 20.183776932826365 34.386882129277566 33.99081115335868 5 9.610662230671736 42.22512674271229 30.610345373891 17.55386565272497 6 25.093076679340935 37.468314321926485 14.35757287705957 23.081036121673005 7 19.276774397972115 41.086422686945504 18.918330164765525 20.718472750316856 8 26.386248415716096 28.477503168567807 18.567807351077313 26.568441064638783 9 24.09695817490494 19.55204372623574 21.18979721166033 35.16120088719899 10-11 23.55830164765526 28.13688212927757 22.101750633713564 26.203065589353614 12-13 30.342997465145753 27.32592680608365 24.06923320659062 18.261842522179975 14-15 25.880267743979722 27.491286438529784 28.680489543726235 17.94795627376426 16-17 16.936498034439396 32.36788165047678 28.92988345265326 21.76573686243056 18-19 16.410210709759188 29.656804499366284 34.185876108998734 19.74710868187579 20-21 20.044161913814957 26.694193599493026 32.85804816223067 20.403596324461343 22-23 18.305410329531053 28.365613117870726 33.60959283903676 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17.710313688212928 28.655735107731306 33.72049271229404 19.913458491761723 50-51 15.488355513307987 30.026140684410645 34.705719264892274 19.7797845373891 52-53 16.760733523447403 31.386644486692017 32.089670468948036 19.762951520912548 54-55 15.217046894803548 29.409260139416983 32.186707858048166 23.186985107731306 56-57 18.353929024081115 29.449857414448672 29.88553548795944 22.310678073510772 58-59 18.562050083670822 29.733342575080947 30.82551910566288 20.87908823558535 60-61 17.090657028271526 31.70470862009209 30.41441798286874 20.790216368767638 62-63 15.88474106347163 31.842756708585007 29.95445093573621 22.31805129220715 64-65 15.370524397972115 33.33234315589353 28.93595532319392 22.361177122940433 66-67 16.70445881316157 30.191799269241816 28.356982305353945 24.746759612242673 68-69 16.49041508238276 29.703342839036758 29.689480354879592 24.11676172370089 70-71 18.268773764258555 28.546815589353614 28.516120088719898 24.668290557667934 72-73 17.601394169835235 26.24960392902408 30.98463244613435 25.164369455006337 74-75 16.982533269961976 28.364622940430923 29.01913022813688 25.633713561470216 76-77 17.830388061478622 25.397140390740503 30.364247938640442 26.40822360914043 78-79 17.673677122940433 25.841650823827628 31.263862484157162 25.220809569074774 80-81 18.1469819391635 25.75154467680608 30.033071926489225 26.068401457541192 82-83 16.940945817490494 25.648566223067178 30.874722750316856 26.535765209125472 84-85 17.476631812420788 25.844621356147023 29.447877059569077 27.23086977186312 86-87 17.885921006987463 25.803062589821103 28.827989494028444 27.483026909162987 88-89 18.092474635556925 24.970933987856867 28.00671747836197 28.92987389822424 90-91 18.676536340603107 23.407024690744695 28.713796015698744 29.20264295295345 92-93 19.26093155893536 24.824738593155892 28.204214195183773 27.71011565272497 94-95 18.670785804816223 23.713759505703422 28.345809569074774 29.269645120405574 96-97 17.55683618504436 24.10884030418251 28.764654626109 29.56966888466413 98-99 19.392625158428388 24.273209759188845 26.60507762991128 29.729087452471482 100-101 19.169835234474018 22.70971958174905 28.002217997465145 30.118227186311785 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 521.0 1 329.5 2 153.0 3 163.5 4 130.5 5 121.5 6 193.5 7 330.5 8 461.5 9 460.0 10 343.0 11 243.5 12 209.0 13 208.5 14 223.5 15 231.5 16 237.0 17 232.5 18 230.5 19 241.0 20 252.5 21 280.5 22 327.5 23 389.5 24 413.5 25 432.0 26 506.5 27 608.0 28 675.0 29 752.5 30 821.0 31 870.5 32 942.5 33 971.5 34 1030.5 35 1156.5 36 1181.5 37 1054.5 38 893.0 39 760.5 40 717.5 41 707.5 42 695.0 43 710.0 44 692.5 45 722.5 46 996.0 47 1963.5 48 3826.5 49 3832.0 50 2596.0 51 2308.0 52 2124.5 53 1835.0 54 1183.0 55 662.0 56 477.5 57 428.5 58 427.0 59 409.0 60 426.0 61 437.0 62 399.5 63 364.0 64 316.0 65 282.5 66 254.0 67 229.5 68 217.0 69 181.5 70 146.5 71 129.5 72 117.5 73 99.0 74 71.5 75 52.5 76 38.0 77 30.5 78 23.5 79 14.0 80 8.0 81 8.0 82 7.0 83 2.5 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3208174904942966 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002970532319391635 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.001980354879594423 32-33 0.0 34-35 0.00594106463878327 36-37 0.01782319391634981 38-39 0.012872306717363752 40-41 0.007921419518377692 42-43 0.006931242078580481 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 9.901774397972115E-4 60-61 0.006931242078580481 62-63 0.001980354879594423 64-65 0.0 66-67 9.901774397972115E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.20694708491761724 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.09604721166032953 88-89 0.355473700887199 90-91 0.34359157160963244 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 50496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.960155259822564 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4299329481719 40.11802915082382 2 7.540167840425083 7.081749049429657 3 2.6019482984017204 3.6656368821292777 4 1.2229578712098848 2.297211660329531 5 0.8729388942774006 2.049667300380228 6 0.4259267068696495 1.2000950570342206 7 0.31206511196390163 1.0258238276299114 8 0.2530257664572176 0.9505703422053232 9 0.17711803652005229 0.748574144486692 >10 0.8560705098469195 7.6204055766793415 >50 0.1138615949057479 3.7210868187579216 >100 0.16024965208957112 15.458650190114067 >500 0.025302576645721754 9.260139416983524 >1k 0.008434192215240586 4.802360583016477 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 2.6972433460076046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1063 2.105117237008872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 979 1.9387674271229405 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 926 1.8338086185044358 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 832 1.6476552598225602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 774 1.5327946768060836 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 662 1.3109949302915083 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 503 0.9961185044359949 TruSeq Adapter, Index 10 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 418 0.8277883396704689 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 381 0.7545152091254753 TruSeq Adapter, Index 10 (96% over 28bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 344 0.6812420785804817 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.6158903675538656 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 298 0.5901457541191382 RNA PCR Primer, Index 10 (95% over 23bp) GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 293 0.580243979721166 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 292 0.5782636248415716 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.5644011406844106 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 274 0.542617237008872 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 268 0.5307351077313055 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 265 0.5247940430925222 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 247 0.48914765525982257 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 237 0.46934410646387836 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 226 0.44756020278833963 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 225 0.4455798479087452 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 212 0.4198352344740177 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 199 0.3940906210392902 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.3901299112801014 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 194 0.38418884664131814 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 191 0.37824778200253484 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 181 0.35844423320659063 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 177 0.3505228136882129 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.34062103929024085 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 169 0.33467997465145755 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 156 0.30893536121673004 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 155 0.3069550063371356 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.2970532319391635 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.271308618504436 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 135 0.2673479087452471 TruSeq Adapter, Index 10 (96% over 26bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 125 0.24754435994930293 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 123 0.24358365019011408 No Hit CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 115 0.2277408111533587 RNA PCR Primer, Index 10 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 113 0.22378010139416982 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 112 0.2217997465145754 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 110 0.21783903675538654 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 109 0.21585868187579213 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 107 0.21189797211660333 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 103 0.20397655259822559 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 96 0.19011406844106463 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 90 0.1782319391634981 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.1782319391634981 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 88 0.17427122940430925 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 87 0.17229087452471484 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 83 0.16436945500633715 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 81 0.1604087452471483 TruSeq Adapter, Index 10 (95% over 24bp) TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 77 0.1524873257287706 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 74 0.14654626108998733 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 70 0.13862484157160965 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 69 0.1366444866920152 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 69 0.1366444866920152 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 69 0.1366444866920152 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 68 0.1346641318124208 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 67 0.13268377693282637 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 65 0.12872306717363752 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 61 0.12080164765525983 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 60 0.1188212927756654 No Hit TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT 60 0.1188212927756654 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.11684093789607099 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 59 0.11684093789607099 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.11684093789607099 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 58 0.11486058301647656 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 56 0.1108998732572877 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 56 0.1108998732572877 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 54 0.10693916349809886 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 54 0.10693916349809886 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.003960709759188847 0.0 22-23 0.0 0.0 0.0 0.015842839036755388 0.0 24-25 0.0 0.0 0.0 0.026734790874524714 0.0 26-27 0.0 0.0 0.0 0.05743029150823828 0.0 28-29 0.0 0.0 0.0 0.21684885931558934 0.0 30-31 0.0 0.0 0.0 0.5386565272496832 0.0 32-33 0.0 0.0 0.0 1.0000792141951838 0.0 34-35 0.0 0.0 0.0 1.5516080481622305 0.0 36-37 0.0 0.0 0.0 2.257604562737643 0.0 38-39 0.0 0.0 0.0 3.284418567807351 0.0 40-41 0.0 0.0 0.0 4.25083174904943 0.0 42-43 0.0 0.0 0.0 4.991484474017744 0.0 44-45 0.0 0.0 0.0 5.627178390367554 0.0 46-47 0.0 0.0 0.0 6.401497148288973 0.0 48-49 0.0 0.0 0.0 7.107493662864385 0.0 50-51 0.0 0.0 0.0 7.7877455640050695 0.0 52-53 0.0 0.0 0.0 8.29867712294043 0.0 54-55 0.0 0.0 0.0 8.812579214195184 0.0 56-57 0.0 0.0 0.0 9.459165082382762 0.0 58-59 0.0 0.0 0.0 9.988910012674271 0.0 60-61 0.0 0.0 0.0 10.583016476552597 0.0 62-63 0.0 0.0 0.0 11.116722116603295 0.0 64-65 0.0 0.0 0.0 11.634584917617236 0.0 66-67 0.0 0.0 0.0 12.221760139416983 0.0 68-69 0.0 0.0 0.0 12.931717363751584 0.0 70-71 0.0 0.0 0.0 13.669399556400506 0.0 72-73 0.0 0.0 0.0 14.320936311787072 0.0 74-75 0.0 0.0 0.0 15.080402408111533 0.0 76-77 0.0 0.0 0.0 15.743821292775666 0.0 78-79 0.0 0.0 0.0 16.50922845373891 0.0 80-81 0.0 0.0 0.0 17.29047845373891 0.0 82-83 0.0 0.0 0.0 18.020239226869457 0.0 84-85 0.0 0.0 0.0 18.81832224334601 0.0 86-87 0.0 0.0 0.0 19.569866920152094 0.0 88-89 0.0 0.0 0.0 20.342205323193916 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 20 0.0020296436 71.539085 1 AGCTGGC 20 0.0020700938 71.184586 2 GAGTAAA 30 0.00395177 31.637594 12-13 CACCACT 35 0.008192349 27.252987 84-85 ATGGGAG 35 0.008393495 27.117939 18-19 GAGTACT 875 0.0 21.151993 12-13 GTACTTT 850 0.0 20.657488 14-15 AGTACTT 800 0.0 20.168966 12-13 ACTTTTT 930 0.0 19.135643 16-17 TACTTTT 880 0.0 18.605062 14-15 GGGCTTG 155 9.510408E-4 18.370216 7 TGGGCTT 155 9.510408E-4 18.370216 6 CATGGGG 130 0.007505547 18.25246 6 GGCTTGA 160 0.0011769519 17.796146 8 ATGGGCT 185 0.0031069699 15.391264 5 CATGGGC 190 0.0037106944 14.986229 4 GCTTGAT 195 0.004410297 14.601967 9 CTTGAAA 405 0.0 13.475272 56-57 TGCTTGA 420 0.0 12.994013 54-55 GTACATG 845 0.0 12.981453 1 >>END_MODULE