FastQCFastQC Report
Fri 27 May 2016
SRR939181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272964
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.4860310268004437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49630.3898774827882014No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44790.35185598335852386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.24933933716900086No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26910.21139639455632683No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17160.13480349797794752No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16400.1288331798856841No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13190.10361644162757155No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.10322365754255422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13900.030.677162
GTATAGG3550.025.4088441
ATGGGTA4700.025.2016855
GTACCTG17000.024.8542521
GTACATG75750.024.4422761
GTACACG2602.3646862E-1123.7372071
TACATGG76550.023.6431662
ACATGGG77500.022.55863
GAGTACT55050.022.02458812-13
GTACTTT58000.020.86518514-15
GTATCAA130350.020.8326571
ACCTGGG20250.020.8234373
CATGGGG34250.020.3350034
AGTACTT56000.020.12862412-13
GTACCGG1200.00477863519.7810081
CATGGGA41000.019.2983444
GGTATCA102800.019.0267121
CAACGCA149100.018.2080925
ATCAACG148500.018.1859443
TATCAAC148800.018.181122
Produced by FastQC (version 0.11.5)