##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1272964 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.198900361675584 33.0 31.0 34.0 31.0 34.0 2 32.28014067954789 34.0 31.0 34.0 30.0 34.0 3 32.33381383919734 34.0 31.0 34.0 30.0 34.0 4 35.7662329806656 37.0 35.0 37.0 35.0 37.0 5 35.818759210786794 37.0 35.0 37.0 35.0 37.0 6 35.911016336675665 37.0 37.0 37.0 35.0 37.0 7 35.84073940818436 37.0 37.0 37.0 35.0 37.0 8 35.83869221753325 37.0 37.0 37.0 35.0 37.0 9 37.53806313454269 39.0 38.0 39.0 35.0 39.0 10-11 37.44704170738528 39.0 38.0 39.0 35.0 39.0 12-13 37.36353659647877 39.0 38.0 39.0 35.0 39.0 14-15 38.67930554202633 40.0 38.0 41.0 34.5 41.0 16-17 38.631357995984175 40.0 38.0 41.0 34.0 41.0 18-19 38.57912399722223 40.0 38.0 41.0 34.0 41.0 20-21 38.49151547097954 40.0 38.0 41.0 34.0 41.0 22-23 38.4044089227975 40.0 38.0 41.0 34.0 41.0 24-25 38.42978120355328 40.0 38.0 41.0 34.0 41.0 26-27 38.34943407669031 40.0 38.0 41.0 34.0 41.0 28-29 38.233431974509884 40.0 38.0 41.0 34.0 41.0 30-31 38.05754993856857 40.0 38.0 41.0 33.5 41.0 32-33 37.840145911431904 40.0 38.0 41.0 33.0 41.0 34-35 37.518283313589386 40.0 37.0 41.0 32.5 41.0 36-37 37.25947748718738 40.0 37.0 41.0 31.5 41.0 38-39 37.037305846826776 40.0 36.5 41.0 31.0 41.0 40-41 36.89032604221329 40.0 36.5 41.0 31.0 41.0 42-43 36.67159283373293 40.0 36.0 41.0 30.5 41.0 44-45 36.3823745211962 40.0 36.0 41.0 30.0 41.0 46-47 35.9638143733837 39.0 35.0 41.0 28.5 41.0 48-49 35.88585969438256 39.5 35.5 41.0 28.0 41.0 50-51 34.86983449649794 38.5 34.0 40.0 25.5 40.5 52-53 35.02322060953806 38.5 35.0 40.0 26.5 40.5 54-55 35.51829116927109 39.0 35.0 41.0 27.0 41.0 56-57 35.39297065745771 39.0 35.0 41.0 26.5 41.0 58-59 35.20891557027536 39.0 35.0 41.0 26.0 41.0 60-61 34.913999531801366 38.5 35.0 41.0 26.0 41.0 62-63 34.56331483058437 38.0 34.5 40.0 25.5 41.0 64-65 34.1890190924488 37.5 34.0 40.0 24.5 41.0 66-67 33.7953535999447 37.0 34.0 40.0 23.0 41.0 68-69 33.404274590640426 36.0 34.0 39.0 22.0 41.0 70-71 32.994111380997424 36.0 33.5 39.0 21.5 41.0 72-73 32.54403659490764 35.0 33.0 38.5 20.0 40.0 74-75 32.103572057025964 35.0 33.0 37.0 18.5 39.5 76-77 31.686387046295103 35.0 32.0 37.0 17.5 39.0 78-79 31.278021216625135 35.0 32.0 36.0 12.5 39.0 80-81 30.86944642582194 35.0 31.5 36.0 7.5 37.5 82-83 30.4739701986859 35.0 31.0 35.5 4.5 37.0 84-85 30.11725665454797 35.0 31.0 35.0 2.0 37.0 86-87 29.773925264186573 34.0 31.0 35.0 2.0 36.0 88-89 29.404646164384854 34.0 30.0 35.0 2.0 36.0 90-91 29.059732246944925 34.0 30.0 35.0 2.0 36.0 92-93 28.68919977312791 34.0 29.0 35.0 2.0 35.0 94-95 28.276104430290253 34.0 29.0 35.0 2.0 35.0 96-97 27.76419718075295 34.0 29.0 35.0 2.0 35.0 98-99 27.131351711438814 34.0 27.0 35.0 2.0 35.0 100-101 24.679250945038508 31.5 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17920022119866985 1101 2 0.11548444624853005 1101 3 0.10258732856632236 1101 4 0.0322161207961571 1101 5 0.22336830298225152 1101 6 0.21346722525461814 1101 7 0.16887035960961327 1101 8 0.32022186390230445 1101 9 0.2605278964565656 1101 10-11 0.2822779272616387 1101 12-13 0.2356647498454194 1101 14-15 0.2322632730313714 1101 16-17 0.42117183633301636 1101 18-19 0.41397899340327626 1101 20-21 0.2897309132488317 1101 22-23 0.1507350787900279 1101 24-25 0.3249008817570811 1101 26-27 0.3674691611780645 1101 28-29 0.30816916834834274 1101 30-31 0.35214982906060754 1101 32-33 0.2689028769978492 1101 34-35 0.19625008673872202 1101 36-37 0.19184694419245574 1101 38-39 0.3149197394496852 1101 40-41 0.09762891397631535 1101 42-43 0.07679371579177285 1101 44-45 0.0026124680459673755 1101 46-47 -0.07372347305568638 1101 48-49 0.04331204170494374 1101 50-51 -0.12427646256505653 1101 52-53 -0.07503605105689104 1101 54-55 -0.2501522786966106 1101 56-57 -0.24732477588481316 1101 58-59 -0.24895084065686746 1101 60-61 -0.2996374523262446 1101 62-63 -0.33570376317452855 1101 64-65 -0.3058128082916127 1101 66-67 -0.30881511719439203 1101 68-69 -0.37957711622416923 1101 70-71 -0.4663064619349413 1101 72-73 -0.5338750662461678 1101 74-75 -0.57880225019818 1101 76-77 -0.5629126473127535 1101 78-79 -0.6763879366861403 1101 80-81 -0.6145065106229453 1101 82-83 -0.5431483762726899 1101 84-85 -0.5819247847272457 1101 86-87 -0.7509414058084083 1101 88-89 -0.6644670339612802 1101 90-91 -0.6936319676049258 1101 92-93 -0.8134189054042054 1101 94-95 -0.9042942005842427 1101 96-97 -0.852015137868456 1101 98-99 -0.935075967749313 1101 100-101 -0.9499408523635964 1102 1 0.20201024503426623 1102 2 0.21110256130361194 1102 3 0.27464513526176404 1102 4 0.3659591687465067 1102 5 0.24236720783311938 1102 6 0.30657809299925276 1102 7 0.42814240753849475 1102 8 0.34847757048822814 1102 9 0.4658550454183157 1102 10-11 0.26508408586499854 1102 12-13 0.2397503003189101 1102 14-15 0.3988049638557314 1102 16-17 0.4200642325622681 1102 18-19 0.4483988864651849 1102 20-21 0.3192161931560662 1102 22-23 0.41455650506072317 1102 24-25 0.19482679813921777 1102 26-27 0.2833694381863694 1102 28-29 0.36488157359647033 1102 30-31 0.4004841287020042 1102 32-33 0.17353127786537215 1102 34-35 0.19290666085454689 1102 36-37 0.19825893500179603 1102 38-39 0.3733650571503091 1102 40-41 0.2566613175065626 1102 42-43 0.08847616549007142 1102 44-45 0.1910970822743181 1102 46-47 0.04229791456268117 1102 48-49 -0.05467202494883594 1102 50-51 -0.04089610223952178 1102 52-53 0.17407086556146112 1102 54-55 0.11293432397899039 1102 56-57 -0.01486885026906748 1102 58-59 -0.07373685710557965 1102 60-61 -0.23499960311325196 1102 62-63 -0.017135616982855595 1102 64-65 0.04911385966345705 1102 66-67 0.07668811456922953 1102 68-69 -0.003575484237231308 1102 70-71 -0.051072293281976044 1102 72-73 -0.10601747858055433 1102 74-75 0.03179836394427582 1102 76-77 -0.2024337879857363 1102 78-79 -0.32213553702112563 1102 80-81 -0.3042108203568894 1102 82-83 -0.432236396200107 1102 84-85 -0.4224410079658796 1102 86-87 -0.5045002110221795 1102 88-89 -0.3834990080212748 1102 90-91 -0.37021767912975534 1102 92-93 -0.40916242577183226 1102 94-95 -0.45937891359075067 1102 96-97 -0.5495997971911848 1102 98-99 -0.8139262758942181 1102 100-101 -0.9507160461620465 1103 1 0.2553318356240126 1103 2 0.30797517762702853 1103 3 0.27897088097616063 1103 4 0.315942106640442 1103 5 0.3261421486175706 1103 6 0.25585094618485016 1103 7 0.23068616682228793 1103 8 0.2929830705083063 1103 9 0.29400952773394806 1103 10-11 0.35140077862664754 1103 12-13 0.38702513070150957 1103 14-15 0.47349684229325106 1103 16-17 0.48635678746209976 1103 18-19 0.43906114709387367 1103 20-21 0.4334745937341751 1103 22-23 0.4996186311998656 1103 24-25 0.5064297830041369 1103 26-27 0.4462372771200904 1103 28-29 0.4077124780888184 1103 30-31 0.44114839663728844 1103 32-33 0.5590596009614401 1103 34-35 0.5026917786834559 1103 36-37 0.3678412144991583 1103 38-39 0.29488940577931544 1103 40-41 0.2693035853615626 1103 42-43 0.23982708282918708 1103 44-45 0.25467726413629777 1103 46-47 0.29480955694094035 1103 48-49 0.30181684453608426 1103 50-51 0.35491590346123303 1103 52-53 0.3638453948480702 1103 54-55 0.3737110512663193 1103 56-57 0.16116064710136868 1103 58-59 0.15497189274387324 1103 60-61 0.12870051531783844 1103 62-63 0.17904598404488326 1103 64-65 0.07118921395307609 1103 66-67 0.024971114689662954 1103 68-69 -0.06893589613654427 1103 70-71 0.11377274264881265 1103 72-73 0.012345863986389816 1103 74-75 0.03596603426423428 1103 76-77 0.05530061524201457 1103 78-79 0.046382117240387544 1103 80-81 -0.04889773071057846 1103 82-83 0.018662375328254655 1103 84-85 -0.1847432882661728 1103 86-87 -0.4020987195933792 1103 88-89 -0.09173573972467608 1103 90-91 -0.20173063629383492 1103 92-93 -0.33719797517164807 1103 94-95 -0.44562635427483954 1103 96-97 -0.5592775241171921 1103 98-99 -0.7051791864887811 1103 100-101 -1.0476363360171206 1104 1 0.22898663056697188 1104 2 0.387800956227089 1104 3 0.44367177520989287 1104 4 0.33324861296174646 1104 5 0.37158618192007253 1104 6 0.4220743504401696 1104 7 0.4167600181944735 1104 8 0.372288497581998 1104 9 0.49995309850175573 1104 10-11 0.4856900169861902 1104 12-13 0.5582496912935468 1104 14-15 0.6014262286638896 1104 16-17 0.5575774935983873 1104 18-19 0.7322599599214996 1104 20-21 0.8092262126055871 1104 22-23 0.7077592417456557 1104 24-25 0.6270260673087904 1104 26-27 0.6987812179156521 1104 28-29 0.6119408160475004 1104 30-31 0.6032957139360846 1104 32-33 0.593788247276585 1104 34-35 0.5676586303639937 1104 36-37 0.6516255191553455 1104 38-39 0.7832489494117567 1104 40-41 0.6904703291173391 1104 42-43 0.6857876132023009 1104 44-45 0.5196036440314487 1104 46-47 0.7922463438666298 1104 48-49 0.5486658269597697 1104 50-51 0.4434816604763441 1104 52-53 0.36767094218078 1104 54-55 0.49312209042758326 1104 56-57 0.4049067926456189 1104 58-59 0.4631067216060316 1104 60-61 0.4892752146702932 1104 62-63 0.37556541050187064 1104 64-65 0.30222529165890677 1104 66-67 0.45093665585525144 1104 68-69 0.2831686367034223 1104 70-71 0.25222479321927693 1104 72-73 0.159952246965112 1104 74-75 0.31396865529414697 1104 76-77 0.4221966220258544 1104 78-79 0.16572570342601622 1104 80-81 0.24904206269059515 1104 82-83 0.250816245201829 1104 84-85 0.31670213264039404 1104 86-87 0.29853533837763635 1104 88-89 0.4132461443332929 1104 90-91 0.21047449784122207 1104 92-93 0.05464599645955559 1104 94-95 0.20244256339398348 1104 96-97 0.1486318190835476 1104 98-99 0.21512030472306165 1104 100-101 0.14857473397979604 1105 1 0.17143620227186318 1105 2 0.3159556029987556 1105 3 0.36093022597898283 1105 4 0.35120937560336074 1105 5 0.3002151770384245 1105 6 0.28220376003766745 1105 7 0.27009657525443487 1105 8 0.20940740704133276 1105 9 0.33385518931874003 1105 10-11 0.3608845919148038 1105 12-13 0.32548856943667914 1105 14-15 0.36312605366158834 1105 16-17 0.3545091966471148 1105 18-19 0.4055525361396164 1105 20-21 0.3843850962751105 1105 22-23 0.3643160714407898 1105 24-25 0.3576261409939647 1105 26-27 0.4780319317408299 1105 28-29 0.6074725006749304 1105 30-31 0.5179164655249195 1105 32-33 0.4867605232275807 1105 34-35 0.43762731416246936 1105 36-37 0.4515368666730737 1105 38-39 0.6183507270667263 1105 40-41 0.6455258232685779 1105 42-43 0.5889330493264495 1105 44-45 0.6335980715897094 1105 46-47 0.3916545597641985 1105 48-49 0.4739030011331167 1105 50-51 0.5791947948596814 1105 52-53 0.6222175480571792 1105 54-55 0.5937679870841279 1105 56-57 0.5128463707979591 1105 58-59 0.6710209704777341 1105 60-61 0.6237633329350061 1105 62-63 0.7522555643196895 1105 64-65 0.7533697146118428 1105 66-67 0.6634792550999791 1105 68-69 0.6347931646577365 1105 70-71 0.4553264787677662 1105 72-73 0.6498458639863927 1105 74-75 0.6392104347912451 1105 76-77 0.7054735401431991 1105 78-79 0.6037449536793105 1105 80-81 0.3396080569799942 1105 82-83 0.42020811220007914 1105 84-85 0.584057295208325 1105 86-87 0.43516953751183607 1105 88-89 0.4830513391384912 1105 90-91 0.6458284169717388 1105 92-93 0.5996526741790014 1105 94-95 0.5107989233460906 1105 96-97 0.43167156302754606 1105 98-99 0.2961371602080014 1105 100-101 0.43586404041803917 1106 1 0.0968324269187093 1106 2 0.05447869522709681 1106 3 0.07205695658947775 1106 4 0.13607840610103494 1106 5 0.12288459754298486 1106 6 0.10581972220483493 1106 7 0.14781968478437335 1106 8 0.07123323039921559 1106 9 0.14276479206681358 1106 10-11 0.20700344669311477 1106 12-13 0.13038852654313615 1106 14-15 0.16502202058669724 1106 16-17 0.24047435555149832 1106 18-19 0.2665882531286954 1106 20-21 0.2822894400578875 1106 22-23 0.2870323504434893 1106 24-25 0.1252079901744807 1106 26-27 0.1341136241256038 1106 28-29 0.009133093759338351 1106 30-31 0.13187525167354863 1106 32-33 0.1820775570125619 1106 34-35 0.16635445712773844 1106 36-37 -0.004971598494840634 1106 38-39 -0.04308076400673144 1106 40-41 -0.07865712672619196 1106 42-43 -0.07223170875753482 1106 44-45 -0.1377670606413659 1106 46-47 0.07636463181815856 1106 48-49 -0.026183146413480074 1106 50-51 -0.09025094119720478 1106 52-53 -0.08048654773601527 1106 54-55 -0.046460394026858864 1106 56-57 -0.08761716788372809 1106 58-59 -0.11111260507799159 1106 60-61 -0.20988395233592883 1106 62-63 -0.0799614554077408 1106 64-65 -0.2213432770869943 1106 66-67 -0.32754950520438086 1106 68-69 -0.27680539498412315 1106 70-71 -0.3045637281665776 1106 72-73 -0.27746471053484356 1106 74-75 -0.15328824055505663 1106 76-77 -0.2488801550098323 1106 78-79 -0.25699014802165365 1106 80-81 -0.20127490130512982 1106 82-83 -0.22738786661988186 1106 84-85 -0.1130616280554797 1106 86-87 -0.2514750696157044 1106 88-89 -0.09990262058482813 1106 90-91 0.11220347959992338 1106 92-93 0.2891030239957111 1106 94-95 0.1678592374422081 1106 96-97 0.3028550284657996 1106 98-99 0.15993284273806907 1106 100-101 0.03249239125121761 1107 1 0.16824171982292313 1107 2 0.2828418771783845 1107 3 0.14734402827609472 1107 4 0.1543343975290341 1107 5 0.14198200016667784 1107 6 0.29444494942634236 1107 7 0.20441217861380068 1107 8 0.18990864540319308 1107 9 0.3093243986615306 1107 10-11 0.27283425759218716 1107 12-13 0.3313920122931293 1107 14-15 0.2215237607627003 1107 16-17 0.283892050422196 1107 18-19 0.31070734891192586 1107 20-21 0.23541332250592717 1107 22-23 0.2884739502988296 1107 24-25 0.30779523176273216 1107 26-27 0.32302831839037793 1107 28-29 0.3053030871602971 1107 30-31 0.36906852794116674 1107 32-33 0.30853990537973885 1107 34-35 0.37400252727997696 1107 36-37 0.3963774933613493 1107 38-39 0.5793654821657626 1107 40-41 0.4146267655461813 1107 42-43 0.41661166460637133 1107 44-45 0.46776493120227514 1107 46-47 0.681902021648618 1107 48-49 0.6840067007830086 1107 50-51 0.5911153432470115 1107 52-53 0.7018038720519257 1107 54-55 0.8173035277185292 1107 56-57 0.8569310003769175 1107 58-59 0.7474857806540527 1107 60-61 0.6915946912039672 1107 62-63 0.6119744246058048 1107 64-65 0.6468621756893853 1107 66-67 0.5988023868574714 1107 68-69 0.7839007021769433 1107 70-71 0.6957760076289645 1107 72-73 0.5474957829491132 1107 74-75 0.6980508650255217 1107 76-77 0.676641459204145 1107 78-79 0.6973661622924148 1107 80-81 0.6300221539581123 1107 82-83 0.7135584949345777 1107 84-85 0.6858657244883908 1107 86-87 0.7348197792204232 1107 88-89 0.6543995749113591 1107 90-91 0.757742650766037 1107 92-93 0.7259098044069603 1107 94-95 0.3073729058195873 1107 96-97 0.03225496126086114 1107 98-99 0.07607815384429273 1107 100-101 0.25863025313562815 1108 1 -0.1893111855753702 1108 2 -0.08621164338401854 1108 3 0.033185493162939395 1108 4 -0.05514760603095681 1108 5 0.1053688189591071 1108 6 0.09370752304697305 1108 7 0.07864001279946109 1108 8 0.1207739591368906 1108 9 -0.025520089390930423 1108 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-0.7303061671022277 2316 90-91 -0.7803399402185143 2316 92-93 -0.9596128170207976 2316 94-95 -1.0435648394118324 2316 96-97 -1.1070335275023595 2316 98-99 -1.1501602771478296 2316 100-101 -0.6386722505416103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4066.0 3 974.0 4 963.0 5 1077.0 6 1248.0 7 1588.0 8 1806.0 9 2196.0 10 2711.0 11 3400.0 12 4762.0 13 7580.0 14 6586.0 15 6824.0 16 8693.0 17 12497.0 18 11913.0 19 6986.0 20 6907.0 21 7001.0 22 7365.0 23 7629.0 24 8558.0 25 9796.0 26 11317.0 27 12953.0 28 15579.0 29 18265.0 30 22462.0 31 27772.0 32 35199.0 33 46519.0 34 65687.0 35 99619.0 36 164541.0 37 276007.0 38 287572.0 39 56312.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18022190252843 18.49122046390635 14.19537670220523 22.13318093135999 2 15.534513581983077 20.723722242802772 40.04004948770161 23.701714687512535 3 19.92890370935459 25.59372087627751 29.3956422063444 25.0817332080235 4 12.93453177770472 16.530966017684083 36.74307431183569 33.79142789277551 5 13.832043461775068 37.531565010942536 33.076198133082016 15.560193394200377 6 31.173864328818823 35.21042596269141 18.068287696813634 15.547422011676135 7 28.534122398397127 32.98244262083673 20.593748918783753 17.889686061982392 8 26.69067214824158 33.61794953658778 19.90053898308817 19.79083933208247 9 27.203155812337044 17.301456504577718 20.623724261132935 34.871663421952306 10-11 25.4763337837365 26.440539901959664 26.62739759093931 21.455728723364523 12-13 26.802812931116843 23.99176894869707 27.340017196512417 21.86540092367367 14-15 23.3779504186169 25.0911442719565 26.669484311940856 24.86142099748574 16-17 22.211280478815933 28.51667305481027 26.956708042181404 22.31533842419239 18-19 22.011953483562486 26.97295191123235 29.593384182099342 21.42171042310582 20-21 23.193330709258987 25.73528804589954 29.25647600446538 21.81490524037609 22-23 22.67271117095492 25.792004140128892 29.34564098853442 22.189643700381765 24-25 22.749785942993384 25.977061584308963 29.349781931268133 21.923370541429517 26-27 22.345487411192426 26.00272322243268 29.714627999320374 21.937161367054514 28-29 21.857487878057327 26.583010284724562 29.738156599492743 21.821345237725364 30-31 22.127463957385128 26.105541490592465 29.510271879800015 22.25672267222239 32-33 22.048494033109762 26.275590151770444 29.43380111886319 22.2421146962566 34-35 22.82176040507351 26.18025581930594 29.107091623919768 21.890892151700786 36-37 22.750756530876647 25.857131917037 29.32158147914517 22.07053007294118 38-39 22.304833300578302 26.344515032190696 29.424079761259247 21.926571905971755 40-41 22.33497150558762 26.316140415666485 29.343523774803398 22.005364303942503 42-43 22.168795006443233 26.424172098521314 28.74910455802168 22.65792833701377 44-45 22.34348288932562 26.384687920987638 28.669532030486167 22.602297159200578 46-47 22.59943006312412 26.744084605506814 28.295640839502255 22.360844491866814 48-49 22.77906414394141 26.705158953404595 28.127360120016974 22.38841678263702 50-51 22.24822691955917 26.98698102832547 28.324523359424436 22.440268692690925 52-53 22.68115060974358 27.17464420439634 27.718028924725918 22.426176261134156 54-55 22.06132408362002 26.744030919387612 28.074532396363054 23.120112600629312 56-57 22.499282783691115 27.162352911464133 27.798396513480313 22.53996779136444 58-59 22.85970280685918 26.576708087276813 27.922180910327587 22.64140819553642 60-61 22.59238460009528 26.645499632449216 28.586301871196323 22.175813896259186 62-63 22.38377863627233 26.73747470789019 28.434941806935022 22.44380484890246 64-65 22.099299851758857 26.94439674145837 28.22479752549807 22.731505881284704 66-67 22.222959202608276 26.932303350602364 27.79611016488094 23.04862728190842 68-69 21.96556077170596 26.931407029917644 28.149048437543804 22.953983760832593 70-71 22.345950718128034 26.904650222529668 27.90642856465995 22.842970494682348 72-73 22.422834059373418 26.629484254918633 28.099685816911563 22.847995868796385 74-75 22.035852392567985 27.3628173369479 27.669168063716533 22.932162206767593 76-77 22.264643096239595 26.8477948513901 27.704528086197534 23.183033966172776 78-79 22.35408427480892 26.924708657918284 27.833000575797485 22.888206491475316 80-81 22.37705108892166 27.24910876271836 27.64988434536239 22.72395580299759 82-83 22.368287577664514 26.983772996412466 27.74698805167411 22.900951374248912 84-85 22.200688534192643 26.9460248241759 27.698699584527002 23.154587057104443 86-87 22.29430727627044 26.83941554266217 27.861154081013584 23.0051231000538 88-89 22.211769368911284 26.778565272849082 27.62982581984606 23.379839538393576 90-91 22.185269782858903 26.69431848018999 27.87318596667173 23.247225770279368 92-93 22.458545028305178 26.640778324224645 27.882700225707076 23.017976421763105 94-95 22.247508466987632 26.517404999569642 27.90516973852426 23.329916794918464 96-97 22.148513518475454 26.444442952898246 28.067117999773373 23.33992552885293 98-99 22.17282390911693 26.630002862303463 27.534964517307063 23.66220871127254 100-101 22.234209397129316 25.850849280157647 28.255021290722677 23.65992003199036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1746.0 1 1069.0 2 615.0 3 752.5 4 561.0 5 495.5 6 777.5 7 1439.0 8 2189.5 9 2583.0 10 2393.5 11 2124.0 12 2147.5 13 2315.5 14 2582.5 15 2702.0 16 2744.0 17 2810.5 18 2851.0 19 2923.5 20 3016.0 21 3317.0 22 3762.0 23 4315.5 24 5003.5 25 5864.0 26 7104.0 27 8487.5 28 9723.0 29 11158.5 30 12953.0 31 15114.5 32 17399.5 33 19299.0 34 21453.0 35 23405.0 36 25354.0 37 28211.5 38 30466.0 39 31536.0 40 32319.0 41 34763.5 42 37944.0 43 40239.5 44 42151.0 45 44087.5 46 45040.0 47 48651.5 48 59853.5 49 61027.5 50 52237.5 51 48162.5 52 45851.0 53 45215.0 54 44080.0 55 40331.0 56 35835.5 57 31905.5 58 28573.5 59 24920.5 60 21207.5 61 17875.5 62 14773.5 63 11814.0 64 9225.5 65 7255.5 66 5462.0 67 4535.5 68 3576.0 69 2372.0 70 1776.5 71 1426.5 72 1177.0 73 946.0 74 704.5 75 582.5 76 427.0 77 233.5 78 149.0 79 98.5 80 58.0 81 43.5 82 35.0 83 24.5 84 20.5 85 17.0 86 10.5 87 7.0 88 7.0 89 9.0 90 7.5 91 4.5 92 4.0 93 3.0 94 3.0 95 3.5 96 2.0 97 2.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34478586982821197 2 0.12074182773432712 3 0.11382882783802213 4 0.1208203845513306 5 0.13967402063216242 6 0.0894762145669477 7 0.09803890762032548 8 0.10290943027454036 9 0.09049745318799275 10-11 0.08959404979245289 12-13 0.09383611791064005 14-15 0.0955643678847163 16-17 0.08456641350423107 18-19 0.100906231440952 20-21 0.11033304948136789 22-23 0.11830656640721968 24-25 0.13280029914435915 26-27 0.1316612252978089 28-29 0.1266728674180888 30-31 0.11649975961613997 32-33 0.11041160629837135 34-35 0.334337813166751 36-37 0.4970289811809289 38-39 0.4700054361317366 40-41 0.4476167432857488 42-43 0.48167112345675134 44-45 0.4890554642550771 46-47 0.497461043674448 48-49 0.5087732253229471 50-51 0.4958899073343787 52-53 0.5000926970440641 54-55 0.48595246998344016 56-57 0.4644671805329923 58-59 0.4430604478995478 60-61 0.4015824485217178 62-63 0.4727156463183562 64-65 0.506416520812843 66-67 0.5133687991176498 68-69 0.5266841795997373 70-71 0.5197319012949305 72-73 0.5110120946075458 74-75 0.5188677763078925 76-77 0.4942794925858076 78-79 0.473344100854384 80-81 0.4749152371944533 82-83 0.4884270097190494 84-85 0.48823061767654075 86-87 0.49101938468016376 88-89 0.5051596117407876 90-91 0.5201639637884496 92-93 0.5105800321140268 94-95 0.5165110717977884 96-97 0.5159218956702625 98-99 0.5107371457480336 100-101 0.497461043674448 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1272964.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.22415608567728 #Duplication Level Percentage of deduplicated Percentage of total 1 81.69289528105638 48.38192781216171 2 10.867726721823438 12.872638873395143 3 3.1095149021013246 5.524751877383651 4 1.336896592325147 3.167062898170983 5 0.7193576344542205 2.1301674412170164 6 0.4749682437590102 1.6877756042474177 7 0.29114283542491803 1.2069882109902397 8 0.233125701361409 1.104533834000886 9 0.16203143893265623 0.8636557707121066 >10 0.9754225523442517 10.82048258638556 >50 0.08494164832114638 3.466750032699564 >100 0.04758970187602768 5.125418021951909 >500 0.002791565776478281 1.2281518025740572 >1k 0.0014622487400600518 1.9326070801017918 >5k 1.3293170364182293E-4 0.4870881540080571 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6187 0.4860310268004437 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4963 0.3898774827882014 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4479 0.35185598335852386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3174 0.24933933716900086 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2691 0.21139639455632683 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1716 0.13480349797794752 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1640 0.1288331798856841 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1319 0.10361644162757155 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1314 0.10322365754255422 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.855681700346592E-5 0.0 4 0.0 0.0 0.0 7.855681700346592E-5 0.0 5 0.0 0.0 0.0 7.855681700346592E-5 0.0 6 0.0 0.0 0.0 7.855681700346592E-5 0.0 7 0.0 0.0 0.0 7.855681700346592E-5 0.0 8 0.0 0.0 0.0 7.855681700346592E-5 0.0 9 0.0 0.0 0.0 7.855681700346592E-5 0.0 10-11 0.0 0.0 0.0 7.855681700346592E-5 0.0 12-13 0.0 0.0 0.0 7.855681700346592E-5 0.0 14-15 0.0 0.0 0.0 1.1783522550519888E-4 0.0 16-17 0.0 0.0 0.0 1.5711363400693185E-4 0.0 18-19 0.0 0.0 0.0 2.3567045101039778E-4 0.0 20-21 0.0 0.0 0.0 3.142272680138637E-4 0.0 22-23 0.0 0.0 0.0 5.891761275259944E-4 0.0 24-25 3.927840850173296E-5 0.0 0.0 0.001178352255051989 0.0 26-27 7.855681700346592E-5 0.0 0.0 0.0019246420165849154 0.0 28-29 7.855681700346592E-5 0.0 0.0 0.00463485220320449 0.0 30-31 7.855681700346592E-5 0.0 0.0 0.012019193001530287 0.0 32-33 7.855681700346592E-5 0.0 0.0 0.0247846757645935 0.0 34-35 7.855681700346592E-5 0.0 0.0 0.04721264701908302 0.0 36-37 7.855681700346592E-5 0.0 0.0 0.08558765212527614 0.0 38-39 7.855681700346592E-5 0.0 0.0 0.1385742251941139 0.0 40-41 2.3567045101039778E-4 0.0 0.0 0.20141967879688666 0.0 42-43 2.3567045101039778E-4 0.0 0.0 0.26088718926851034 0.0 44-45 2.3567045101039778E-4 0.0 0.0 0.3330809040946955 0.0 46-47 2.3567045101039778E-4 0.0 0.0 0.42397114136770564 0.0 48-49 2.3567045101039778E-4 0.0 0.0 0.5208316967329791 0.0 50-51 2.3567045101039778E-4 0.0 0.0 0.6290829905637552 0.0 52-53 2.3567045101039778E-4 0.0 0.0 0.7369415003095139 0.0 54-55 2.3567045101039778E-4 0.0 0.0 0.8602364245964536 0.0 56-57 2.3567045101039778E-4 0.0 0.0 1.0066270530824124 0.0 58-59 2.3567045101039778E-4 0.0 0.0 1.1728532778617464 0.0 60-61 2.3567045101039778E-4 0.0 0.0 1.3531411728847007 0.0 62-63 2.3567045101039778E-4 0.0 0.0 1.5428951643565725 0.0 64-65 2.3567045101039778E-4 0.0 0.0 1.7351629739725554 0.0 66-67 2.3567045101039778E-4 0.0 0.0 1.964077538720655 0.0 68-69 2.3567045101039778E-4 0.0 0.0 2.230267313136899 0.0 70-71 2.3567045101039778E-4 0.0 0.0 2.521281041726239 0.0 72-73 2.3567045101039778E-4 0.0 0.0 2.8153584861787135 0.0 74-75 2.3567045101039778E-4 0.0 0.0 3.1229477031557846 0.0 76-77 2.3567045101039778E-4 0.0 0.0 3.4666730559544496 0.0 78-79 2.3567045101039778E-4 0.0 0.0 3.854390226275056 0.0 80-81 2.3567045101039778E-4 0.0 0.0 4.2732158961290345 0.0 82-83 2.3567045101039778E-4 0.0 0.0 4.707556537341198 0.0 84-85 2.3567045101039778E-4 0.0 0.0 5.145628627361026 0.0 86-87 2.3567045101039778E-4 0.0 0.0 5.610684984021543 0.0 88-89 2.3567045101039778E-4 0.0 0.0 6.116630163932365 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1390 0.0 30.67716 2 GTATAGG 355 0.0 25.408844 1 ATGGGTA 470 0.0 25.201685 5 GTACCTG 1700 0.0 24.854252 1 GTACATG 7575 0.0 24.442276 1 GTACACG 260 2.3646862E-11 23.737207 1 TACATGG 7655 0.0 23.643166 2 ACATGGG 7750 0.0 22.5586 3 GAGTACT 5505 0.0 22.024588 12-13 GTACTTT 5800 0.0 20.865185 14-15 GTATCAA 13035 0.0 20.832657 1 ACCTGGG 2025 0.0 20.823437 3 CATGGGG 3425 0.0 20.335003 4 AGTACTT 5600 0.0 20.128624 12-13 GTACCGG 120 0.004778635 19.781008 1 CATGGGA 4100 0.0 19.298344 4 GGTATCA 10280 0.0 19.026712 1 CAACGCA 14910 0.0 18.208092 5 ATCAACG 14850 0.0 18.185944 3 TATCAAC 14880 0.0 18.18112 2 >>END_MODULE