FastQCFastQC Report
Fri 27 May 2016
SRR939181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272964
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.4780182314660901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50920.40001131218164854No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49500.38885624416715636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.25601666661429545No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT24710.1941138948155643No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16560.13009008895773957No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15330.12042760046631328No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.1062873734056894No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13250.10408778252959236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15650.030.0130162
GTATAGG3500.027.2042851
GTACCTG18850.025.255971
GTACATG75150.022.8693371
TACATGG76100.022.0702552
GTACCCG1954.1312524E-721.9726921
CAACGGT1100.002897009721.5658254
ACCTGGG21000.021.4631293
ACATGGG75300.021.2336033
TGCACCG3800.021.2253135
GAGTACT57600.020.55279712-13
CATGGGG29750.020.2537524
GCACCGT3800.019.9767656
AGTACTT58150.019.8688212-13
GTAAGGT2401.430908E-719.7686734
AATTCGC2350.019.1805778-79
TACCCGG2251.6865815E-618.9779262
GTACTTT62200.018.76582114-15
GTATCAA138100.018.4086931
ATTCGCG2350.018.19572480-81
Produced by FastQC (version 0.11.5)