##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939181_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1272964 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.537376547962076 34.0 31.0 34.0 31.0 34.0 2 32.73140324471077 34.0 31.0 34.0 31.0 34.0 3 32.819667327591354 34.0 31.0 34.0 31.0 34.0 4 36.26643094384445 37.0 37.0 37.0 35.0 37.0 5 36.23507106249666 37.0 37.0 37.0 35.0 37.0 6 36.283253886205735 37.0 37.0 37.0 35.0 37.0 7 36.23817955574549 37.0 37.0 37.0 35.0 37.0 8 36.23038593393058 37.0 37.0 37.0 35.0 37.0 9 37.97682731012032 39.0 38.0 39.0 35.0 39.0 10-11 37.91854286531277 39.0 38.0 39.0 35.0 39.0 12-13 37.85135125580928 39.0 38.0 39.0 35.0 39.0 14-15 39.20881344641325 41.0 39.0 41.0 36.0 41.0 16-17 39.16335104527701 40.0 39.0 41.0 36.0 41.0 18-19 39.208996483796874 40.5 38.5 41.0 36.0 41.0 20-21 39.27493903991001 41.0 39.0 41.0 36.0 41.0 22-23 39.229418506729175 41.0 39.0 41.0 36.0 41.0 24-25 39.16937949541385 41.0 39.0 41.0 35.5 41.0 26-27 39.0315664857765 40.5 39.0 41.0 35.0 41.0 28-29 38.8830116169821 40.0 38.0 41.0 35.0 41.0 30-31 38.68516745171112 40.0 38.0 41.0 35.0 41.0 32-33 38.52964537881668 40.0 38.0 41.0 34.5 41.0 34-35 38.46616715005295 40.0 38.0 41.0 34.0 41.0 36-37 38.34969724202727 40.0 38.0 41.0 34.0 41.0 38-39 38.215268852850514 40.0 38.0 41.0 34.0 41.0 40-41 38.04764588786486 40.0 38.0 41.0 33.5 41.0 42-43 37.868703671117174 40.0 38.0 41.0 33.0 41.0 44-45 37.617462080624435 40.0 37.0 41.0 33.0 41.0 46-47 37.266041694816195 40.0 37.0 41.0 32.5 41.0 48-49 36.915274901725425 40.0 37.0 41.0 31.0 41.0 50-51 36.646138853887464 40.0 36.0 41.0 31.0 41.0 52-53 36.41756051231614 39.5 36.0 41.0 30.5 41.0 54-55 36.1738768731873 39.0 35.0 41.0 30.0 41.0 56-57 35.90502795051549 39.0 35.0 41.0 29.5 41.0 58-59 35.6107674686794 38.5 35.0 40.0 28.5 41.0 60-61 35.24778312662416 38.0 35.0 40.0 28.0 41.0 62-63 34.87092918574288 37.5 34.0 40.0 27.5 41.0 64-65 34.46515612381811 37.0 34.0 40.0 26.5 41.0 66-67 34.105005326152195 36.5 34.0 39.0 26.0 41.0 68-69 33.63969758767726 36.0 33.5 39.0 26.0 40.0 70-71 33.20799017097106 35.5 33.0 38.5 25.5 40.0 72-73 32.75385124795359 35.0 33.0 37.5 24.5 40.0 74-75 32.274462592814885 35.0 32.0 37.0 22.5 39.0 76-77 30.308941965365868 33.5 29.5 35.0 20.0 37.5 78-79 31.271672254674918 34.5 31.5 36.0 20.0 38.0 80-81 31.296461251064443 35.0 32.0 36.0 20.0 37.0 82-83 31.01937328942531 35.0 31.5 35.0 19.5 37.0 84-85 30.6591042637498 34.5 31.0 35.0 18.0 36.5 86-87 30.145229951514732 34.0 31.0 35.0 10.0 36.0 88-89 29.63075860747044 34.0 30.0 35.0 4.5 36.0 90-91 29.20569199128962 34.0 29.5 35.0 2.0 35.0 92-93 28.94924051269321 34.0 29.0 35.0 2.0 35.0 94-95 28.658231890296975 34.0 29.0 35.0 2.0 35.0 96-97 28.146055583661436 34.0 29.0 35.0 2.0 35.0 98-99 27.438789313759067 34.0 28.5 35.0 2.0 35.0 100-101 23.92216315622437 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23350103898427932 1101 2 0.19336451237044372 1101 3 0.2408839387196906 1101 4 0.1861890527627139 1101 5 0.12043863028048207 1101 6 0.150265277815393 1101 7 0.17239841897970365 1101 8 0.1942520182209222 1101 9 0.2544968841321378 1101 10-11 0.14798449384512935 1101 12-13 0.2579250431160389 1101 14-15 0.32688594430267415 1101 16-17 0.4100505240515062 1101 18-19 0.32612721036651493 1101 20-21 0.3032598705328482 1101 22-23 0.31075675079381426 1101 24-25 0.23291986403195608 1101 26-27 0.25022423980628616 1101 28-29 0.1769197966768914 1101 30-31 0.2571215878436064 1101 32-33 0.19264258746155605 1101 34-35 0.3328082367675691 1101 36-37 0.4419105145837747 1101 38-39 0.41256525372393327 1101 40-41 0.3611614279225037 1101 42-43 0.21925073242188375 1101 44-45 0.1013163604912819 1101 46-47 0.1647841546581148 1101 48-49 0.03679189892574897 1101 50-51 0.041498350499189485 1101 52-53 0.1395927517257931 1101 54-55 0.2125723389156704 1101 56-57 0.18934496350870944 1101 58-59 0.01444775446933022 1101 60-61 0.21929221725975623 1101 62-63 0.2103310841333368 1101 64-65 0.14454253142437778 1101 66-67 0.07250599598736329 1101 68-69 0.09496459289118775 1101 70-71 0.18436488988685795 1101 72-73 0.10737500192447413 1101 74-75 0.23122349919739804 1101 76-77 0.27427750951521546 1101 78-79 0.445144813613517 1101 80-81 0.3327036682087048 1101 82-83 0.24138279712259347 1101 84-85 0.24595379329537082 1101 86-87 0.3340718645627092 1101 88-89 0.3850463988373356 1101 90-91 0.6132283572561192 1101 92-93 0.524471522823589 1101 94-95 0.3763910462341862 1101 96-97 0.35435002869905574 1101 98-99 0.2372958544687762 1101 100-101 0.007201238479762395 1102 1 0.1861364831638781 1102 2 0.2131398994305087 1102 3 0.24584002538679073 1102 4 0.12735162872526473 1102 5 0.06788349703733587 1102 6 0.09341200632577085 1102 7 0.18312916260743606 1102 8 0.1522000093738356 1102 9 0.23508396996177083 1102 10-11 0.33293413845849784 1102 12-13 0.23201510302714468 1102 14-15 0.3093674233982142 1102 16-17 0.26711423596287176 1102 18-19 0.15811114817922345 1102 20-21 0.14828553138018918 1102 22-23 0.25815738211507977 1102 24-25 0.20578593373497256 1102 26-27 0.20412103810558335 1102 28-29 0.28850346821804607 1102 30-31 0.23939434439839857 1102 32-33 0.161287823562823 1102 34-35 0.3026714504934347 1102 36-37 0.3499497681644854 1102 38-39 0.4048304945493797 1102 40-41 0.38922644971811593 1102 42-43 0.2700072442645265 1102 44-45 0.4138792241988938 1102 46-47 0.4323582919597513 1102 48-49 0.43077716809622046 1102 50-51 0.4891364788678416 1102 52-53 0.4710359864956999 1102 54-55 0.5494591090846725 1102 56-57 0.5892453264110813 1102 58-59 0.553996810708469 1102 60-61 0.4788047685277661 1102 62-63 0.26450145498089483 1102 64-65 0.2483476378256313 1102 66-67 0.2713739124124501 1102 68-69 0.41063896854881676 1102 70-71 0.48641603979344694 1102 72-73 0.5131895996182223 1102 74-75 0.5593649666972311 1102 76-77 0.4950767536475773 1102 78-79 0.4097860985885049 1102 80-81 0.5980351118729494 1102 82-83 0.486268880139189 1102 84-85 0.5515678283830923 1102 86-87 0.834833744783456 1102 88-89 0.7092367400514483 1102 90-91 0.7888292935012622 1102 92-93 0.7534375732004435 1102 94-95 0.8253038423002401 1102 96-97 0.9940571299896135 1102 98-99 0.7325351767647277 1102 100-101 0.1395339061342149 1103 1 0.20286487450837853 1103 2 0.20288507185814098 1103 3 0.2614518524372542 1103 4 0.1495148026784534 1103 5 0.1706425399535476 1103 6 0.17476814266203888 1103 7 0.2065945767147923 1103 8 0.16722219011172257 1103 9 0.2741185558544217 1103 10-11 0.347227415988705 1103 12-13 0.21322922242173803 1103 14-15 0.37605893048400674 1103 16-17 0.38477878492107465 1103 18-19 0.3703549656749132 1103 20-21 0.26654349035084834 1103 22-23 0.2616751869245846 1103 24-25 0.0981604268900611 1103 26-27 0.25039823710996245 1103 28-29 0.35819338953162827 1103 30-31 0.3631836876076804 1103 32-33 0.4094950478592594 1103 34-35 0.4373705050520229 1103 36-37 0.49498896082577204 1103 38-39 0.39898672322174633 1103 40-41 0.44805433321403854 1103 42-43 0.6030946825398473 1103 44-45 0.6011082378084325 1103 46-47 0.5470532817224907 1103 48-49 0.5790694520096693 1103 50-51 0.7098034566670393 1103 52-53 0.8547351514561612 1103 54-55 0.8607384157609701 1103 56-57 0.8829218088069979 1103 58-59 0.7642345761747649 1103 60-61 0.9527060190797769 1103 62-63 0.7374856173318562 1103 64-65 0.7687167815086369 1103 66-67 0.8497755443527168 1103 68-69 0.7478614584119114 1103 70-71 0.6805175693610721 1103 72-73 0.738819642976047 1103 74-75 0.5967623431475175 1103 76-77 0.5073664882816473 1103 78-79 0.6453247091308754 1103 80-81 0.5091942310668109 1103 82-83 0.6650283144801925 1103 84-85 0.645174807788127 1103 86-87 0.8267041530696169 1103 88-89 0.8207739856253404 1103 90-91 1.0328500289784017 1103 92-93 0.8880513172287081 1103 94-95 0.7154426977340158 1103 96-97 0.7508945180822728 1103 98-99 0.39988432767403737 1103 100-101 0.041301339591999664 1104 1 0.11585053593298511 1104 2 0.15946462782483906 1104 3 0.14053757553315194 1104 4 0.09873234816102894 1104 5 0.2034825029507772 1104 6 0.13194513927011542 1104 7 0.12861893687513515 1104 8 0.15660316451813827 1104 9 0.2248778836373333 1104 10-11 0.16914192416015084 1104 12-13 0.12093427946768287 1104 14-15 0.17517433288918483 1104 16-17 0.21208953114371099 1104 18-19 0.26579321729378336 1104 20-21 0.18974345951519211 1104 22-23 0.24995764144200905 1104 24-25 0.21968178057491627 1104 26-27 0.28293947978649214 1104 28-29 0.2657153599294091 1104 30-31 0.21766834687378633 1104 32-33 0.20685667289780696 1104 34-35 0.29958334809858655 1104 36-37 0.22233439715016345 1104 38-39 0.15935520919152424 1104 40-41 0.3014212003124115 1104 42-43 0.18349862950253737 1104 44-45 0.16717360937796855 1104 46-47 0.009973417399329776 1104 48-49 -0.11504093960304118 1104 50-51 -0.1724332840051872 1104 52-53 -0.050233242006683554 1104 54-55 -0.14813646860535812 1104 56-57 -0.06731716744955207 1104 58-59 0.0833835123449802 1104 60-61 0.05204497991849877 1104 62-63 -0.14075212549885663 1104 64-65 -0.20133409730727436 1104 66-67 -0.22161861229236735 1104 68-69 -0.3443756677058758 1104 70-71 -0.28494650711132863 1104 72-73 -0.3136298636536239 1104 74-75 -0.28634165006864265 1104 76-77 -0.23795034798107295 1104 78-79 -0.008207899564773413 1104 80-81 -0.04858406063840093 1104 82-83 -0.007429671952124295 1104 84-85 -0.013195142874842247 1104 86-87 0.08082842072302654 1104 88-89 0.263005330059503 1104 90-91 0.5474391439953585 1104 92-93 0.45337470606620656 1104 94-95 0.33812038814413015 1104 96-97 0.11028397229133802 1104 98-99 0.21338648616925582 1104 100-101 -0.08048018985604344 1105 1 0.15042039869433665 1105 2 0.1519510421197623 1105 3 0.14882715829082116 1105 4 0.12453597715614961 1105 5 0.12288703458934691 1105 6 0.15743330358806418 1105 7 0.19682608433760151 1105 8 0.14972971881490338 1105 9 0.22398445082900764 1105 10-11 0.21552098835836375 1105 12-13 0.2219037001179558 1105 14-15 0.2885953976400373 1105 16-17 0.19619771019869603 1105 18-19 0.2827385060475862 1105 20-21 0.32068662982006657 1105 22-23 0.33270379599667166 1105 24-25 0.23260777302219537 1105 26-27 0.3110054740758912 1105 28-29 0.3301242666255533 1105 30-31 0.29522890688115666 1105 32-33 0.3466927233721506 1105 34-35 0.4155949074612124 1105 36-37 0.4501920005396798 1105 38-39 0.4512947412906314 1105 40-41 0.4130611561012998 1105 42-43 0.39863863134466015 1105 44-45 0.35634303923889377 1105 46-47 0.41052260225702497 1105 48-49 0.33450658733810457 1105 50-51 0.21805326844945228 1105 52-53 0.10715139463327006 1105 54-55 0.15411668604141227 1105 56-57 0.31481763037673005 1105 58-59 0.26493803937822236 1105 60-61 0.3496133689762573 1105 62-63 0.3663181977948682 1105 64-65 0.26420426503959504 1105 66-67 0.3220640348477204 1105 68-69 0.1956169637761178 1105 70-71 0.441260087712287 1105 72-73 0.37834651103552375 1105 74-75 0.29336383947727285 1105 76-77 0.41735613631477264 1105 78-79 0.678534524677648 1105 80-81 0.41109287590024124 1105 82-83 0.37135360151200203 1105 84-85 0.4146118019533809 1105 86-87 0.6992243864594165 1105 88-89 0.8547272606112202 1105 90-91 0.8446206858577519 1105 92-93 0.9441272159676544 1105 94-95 0.7874088656240978 1105 96-97 0.47342180963130076 1105 98-99 0.8225269024905231 1105 100-101 0.6562599317245024 1106 1 0.0626775004601754 1106 2 0.02480807684191433 1106 3 0.06689327202720818 1106 4 -0.03995969924627474 1106 5 -0.0468769788049741 1106 6 0.005169137002503987 1106 7 0.02734178013223243 1106 8 0.0012531606797239192 1106 9 0.06891742515415444 1106 10-11 0.055963116310906 1106 12-13 0.07207797768607094 1106 14-15 -0.037762507568828596 1106 16-17 -0.0317479771974476 1106 18-19 0.06612140125395882 1106 20-21 4.405145697958801E-4 1106 22-23 0.11023586703415589 1106 24-25 0.10691633983699944 1106 26-27 0.06806867177189702 1106 28-29 0.02511761632330689 1106 30-31 0.13534554849655223 1106 32-33 0.19237110826785653 1106 34-35 0.03259949598953682 1106 36-37 0.153527965157906 1106 38-39 0.20050553604680488 1106 40-41 0.12237049788357979 1106 42-43 0.2232195181237202 1106 44-45 0.16966599161263218 1106 46-47 0.3291921092695773 1106 48-49 0.25779958631803623 1106 50-51 0.24308075276086782 1106 52-53 0.28437636228289165 1106 54-55 0.20163300541452145 1106 56-57 0.36603144570378987 1106 58-59 0.30595328280811884 1106 60-61 0.07176554375124056 1106 62-63 0.15194020831280142 1106 64-65 0.19658637086050845 1106 66-67 0.20147332941105844 1106 68-69 0.325220000206798 1106 70-71 0.2961775266430493 1106 72-73 0.1774882796194177 1106 74-75 0.18714860037277958 1106 76-77 0.1233432105782839 1106 78-79 0.3720828303824888 1106 80-81 0.11367766274814173 1106 82-83 0.20637708482857064 1106 84-85 0.2942968407558233 1106 86-87 0.26808150768956196 1106 88-89 0.4085024335371017 1106 90-91 0.39759061351544034 1106 92-93 0.43457240774506545 1106 94-95 0.1909978661323315 1106 96-97 0.3068012805628122 1106 98-99 0.6735330986257821 1106 100-101 0.3048543570050235 1107 1 -0.04456160471554682 1107 2 -0.04708703487617072 1107 3 0.011895208554982162 1107 4 0.009565480338004306 1107 5 0.04833203799221053 1107 6 0.07895624310128113 1107 7 0.03289293482908562 1107 8 -0.018392812354633747 1107 9 0.1846178746715097 1107 10-11 0.07350859772360252 1107 12-13 0.05851114993448192 1107 14-15 0.11789721413796883 1107 16-17 0.2103746731601035 1107 18-19 0.06585959889314807 1107 20-21 0.1467448503677531 1107 22-23 0.09459452547248759 1107 24-25 0.05682774537829971 1107 26-27 0.09804301783952951 1107 28-29 0.04441103334342955 1107 30-31 -0.0026108006830227737 1107 32-33 0.20812108431429266 1107 34-35 0.23480740754892082 1107 36-37 0.17566612123260228 1107 38-39 0.21048843792355143 1107 40-41 0.050658242969056744 1107 42-43 0.1803649007532684 1107 44-45 0.4144127383134517 1107 46-47 0.493082748755576 1107 48-49 0.3574545200105135 1107 50-51 0.37298566722301985 1107 52-53 0.3670067202908669 1107 54-55 0.32104996885800574 1107 56-57 0.24279338233952075 1107 58-59 0.3015454891947513 1107 60-61 0.44663042539363573 1107 62-63 0.2734870149312698 1107 64-65 0.2179246127631842 1107 66-67 0.23351274281183265 1107 68-69 0.08870248441430562 1107 70-71 0.19072650460172014 1107 72-73 0.1992060674704348 1107 74-75 0.20939167981629936 1107 76-77 0.2784273719403032 1107 78-79 0.5637464494357651 1107 80-81 0.5366925868322028 1107 82-83 0.3123054913293686 1107 84-85 0.46235573819002695 1107 86-87 0.5042697462859742 1107 88-89 0.45353345171887227 1107 90-91 0.44861677081007656 1107 92-93 0.42836809664289177 1107 94-95 0.4926731313421726 1107 96-97 0.654658347269784 1107 98-99 0.5023612496668477 1107 100-101 0.21488847228173213 1108 1 -0.1438085102730824 1108 2 -0.04614309828816232 1108 3 -0.09967552761281695 1108 4 -0.09033768589500824 1108 5 0.0038064849224284103 1108 6 0.031804363062136076 1108 7 -0.03677343816841727 1108 8 -0.04806324363732983 1108 9 -0.15145822588149827 1108 10-11 -0.05356196249954337 1108 12-13 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2316 88-89 -0.7780207091805416 2316 90-91 -0.7845029277549145 2316 92-93 -0.8189696020561748 2316 94-95 -0.4546851717254121 2316 96-97 -0.6660432265490606 2316 98-99 -0.7254594785250532 2316 100-101 -0.3805753133224208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 32.0 6 53.0 7 167.0 8 294.0 9 542.0 10 942.0 11 1578.0 12 2573.0 13 3680.0 14 4296.0 15 5667.0 16 7875.0 17 12072.0 18 11734.0 19 7197.0 20 6099.0 21 6534.0 22 6571.0 23 7002.0 24 8019.0 25 9059.0 26 10909.0 27 13198.0 28 16087.0 29 19772.0 30 24217.0 31 29713.0 32 37330.0 33 48997.0 34 69401.0 35 104493.0 36 172123.0 37 305166.0 38 289788.0 39 29762.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30906710243877 18.95971236519089 14.591822336157811 22.139398196212532 2 15.746399741076731 21.03649435490713 39.4650594989332 23.75204640508294 3 19.98210475708661 25.48477411772839 29.542626500042417 24.99049462514258 4 13.161487677577686 16.82137122495216 36.44015070339774 33.57699039407242 5 13.822936076746867 37.65071125342115 33.0304706181793 15.495882051652679 6 31.223978054367603 35.57484736410456 17.719039972850766 15.482134608677073 7 28.646450331666884 33.07194861755713 20.320527524737543 17.961073526038444 8 26.763679098544813 33.79962041346024 19.656722421058255 19.779978066936692 9 27.028258458212484 17.281949843043478 20.463736602134862 35.22605509660917 10-11 25.439996732036413 26.58111305582876 26.505659233096928 21.4732309790379 12-13 26.731235133122382 24.054293758503775 27.312202073271514 21.90226903510233 14-15 23.270277701402254 25.254723280568758 26.52716131819789 24.9478376998311 16-17 22.18091376404373 28.60727911333064 26.903589986515502 22.308217136110127 18-19 21.9107138929302 27.08391596305944 29.540151960306808 21.465218183703545 20-21 23.107487721569502 25.80395046521347 29.220582828736713 21.867978984480317 22-23 22.547809678828308 25.90501381028843 29.295251083298506 22.251925427584755 24-25 22.706208688739142 25.983653820471687 29.415228153639006 21.894909337150164 26-27 22.3577087278422 26.02900285833582 29.72787087565159 21.88541753817039 28-29 21.793114337876013 26.66473678752895 29.687995889907338 21.854152984687705 30-31 22.043407984382394 26.1074810131803 29.532827018141322 22.316283984295982 32-33 21.969749340908308 26.36771346243884 29.448240484412757 22.214296712240095 34-35 22.789574205145488 26.19753575264192 29.148331726693712 21.86455831551888 36-37 22.67463561020802 25.97380809629367 29.35430969655204 21.99724659694627 38-39 22.259356791887473 26.377417746622626 29.45383372416955 21.909391737320348 40-41 22.256787541400143 26.33683521613713 29.384663552514294 22.021713689948434 42-43 22.161225760453473 26.485955646515574 28.75033635347657 22.602482239554384 44-45 22.328024039122493 26.376809317123946 28.66626077734352 22.62890586641004 46-47 22.56460674267637 26.840928346445246 28.238192892659303 22.356272018219077 48-49 22.72390368061652 26.777157827962945 28.147318929810815 22.351619561609724 50-51 22.25519427697652 27.0854191813041 28.25469858326653 22.404687958452854 52-53 22.64518974817424 27.30490701422311 27.632606905068368 22.417296332534278 54-55 22.078589810866607 26.80303606386355 28.014539295690998 23.10383482957884 56-57 22.487478043369645 27.18387951269635 27.71841937399644 22.61022306993756 58-59 22.904531554728052 26.64015256411012 27.82331002056316 22.63200586059867 60-61 22.54966930341397 26.81146582435311 28.44980549454823 22.18905937768469 62-63 22.31665503332295 26.943256123806535 28.21544769257177 22.52464115029874 64-65 22.044453731770076 27.048140075565136 28.039976699636238 22.86742949302855 66-67 22.125916888662985 27.058123886908753 27.734639831756773 23.08131939267149 68-69 21.94950941940664 27.02555245553469 27.962720666996354 23.062217458062324 70-71 22.243480569756883 27.024998350306845 27.781775446909734 22.94974563302654 72-73 22.341401642151702 26.72581471274914 27.913515229024544 23.01926841607461 74-75 21.915780807626923 27.53259322337474 27.439071332732112 23.112554636266225 76-77 22.1003411838019 26.91864220892794 27.644115415941517 23.33690119132865 78-79 22.107886959705002 26.97794592454449 27.78496137355239 23.129205742198117 80-81 22.141297747164217 27.293509692999372 27.558606960796713 23.006585599039695 82-83 22.190166525525587 26.992799448214317 27.656921909442417 23.16011211681768 84-85 21.941900949280576 26.94773772078393 27.531139922260174 23.579221407675316 86-87 22.013113787171296 26.90154829196363 27.661754730892113 23.423583189972966 88-89 21.88584856322723 26.81194916611253 27.423116939870017 23.87908533079022 90-91 21.796107909700098 26.71061229302395 27.72439025640601 23.768889540869946 92-93 22.03652263536125 26.734691633070533 27.670499715624324 23.558286015943892 94-95 21.738949412552124 26.555424976668622 27.645165142140705 24.06046046863855 96-97 21.53489808038562 26.54929754494236 27.866695366090475 24.049109008581546 98-99 21.622702325199565 26.66388833134516 27.306807170304754 24.406602173150517 100-101 21.522492083405673 25.907432996663676 28.051414067538705 24.518660852391946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 660.0 1 469.0 2 511.0 3 661.5 4 486.0 5 423.0 6 649.5 7 1298.5 8 2023.0 9 2363.5 10 2189.5 11 1895.0 12 1920.5 13 2066.5 14 2198.5 15 2260.0 16 2368.0 17 2455.0 18 2496.5 19 2618.0 20 2788.5 21 3119.0 22 3573.5 23 4267.5 24 5018.0 25 5813.0 26 6957.0 27 8419.5 28 9732.0 29 11145.5 30 13078.0 31 15251.0 32 17353.0 33 19494.5 34 21757.0 35 23429.0 36 25484.5 37 28300.5 38 30508.5 39 31527.0 40 32190.5 41 34761.0 42 37927.0 43 40169.5 44 42346.0 45 44390.0 46 45084.0 47 48752.0 48 60316.5 49 61265.5 50 52614.5 51 49217.5 52 46638.0 53 45796.5 54 44704.0 55 40964.0 56 36310.5 57 31974.0 58 28775.5 59 25347.0 60 21559.0 61 17889.5 62 14648.5 63 11785.5 64 9189.5 65 7106.0 66 5294.0 67 4459.5 68 3453.5 69 2275.0 70 1722.0 71 1319.0 72 1047.5 73 846.0 74 673.0 75 600.5 76 390.5 77 180.5 78 114.5 79 72.5 80 35.5 81 15.5 82 13.0 83 9.5 84 6.5 85 6.5 86 4.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3465141198022882 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001099795438048523 16-17 0.0016104147485710514 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.7134090202079556E-4 26-27 0.0016104147485710514 28-29 0.0 30-31 0.003260107905643836 32-33 0.0 34-35 0.009505374857419377 36-37 0.028044783670237338 38-39 0.020856834914420204 40-41 0.013551050933097872 42-43 0.00954465326592111 44-45 0.0030637158631351713 46-47 9.819602125433241E-4 48-49 9.034033955398582E-4 50-51 3.927840850173297E-5 52-53 3.927840850173297E-5 54-55 0.0 56-57 0.0 58-59 0.004438460160695825 60-61 0.009466096448917643 62-63 0.003338664722647302 64-65 0.0017675283825779834 66-67 0.0035350567651559668 68-69 0.002631653369616109 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2221194000772999 78-79 1.5711363400693187E-4 80-81 0.0027494885951213077 82-83 4.7134090202079556E-4 84-85 0.0 86-87 0.1100973790303575 88-89 0.41061648247711646 90-91 0.39541573838694577 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015318579315675856 100-101 3.927840850173297E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1272964.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.068944348037746 #Duplication Level Percentage of deduplicated Percentage of total 1 80.95565490350579 47.010094192506266 2 11.238394038035535 13.052033559120096 3 3.247742086647927 5.657788634590154 4 1.4166376561474257 3.2905061286463795 5 0.7469807609303782 2.168819211776051 6 0.4840637162463024 1.6865441399766523 7 0.3134634287568131 1.2741743279737132 8 0.23420182316807886 1.0879882108604921 9 0.18331318045175976 0.9580322586523535 >10 1.0345426104520943 11.19273245310982 >50 0.0895762466720619 3.5792553740817765 >100 0.05095725255651849 5.324994302164105 >500 0.0025749585502857614 1.1001114310267441 >1k 0.001626289610706797 1.7373245547854927 >5k 2.710482684511328E-4 0.8796012207300893 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6085 0.4780182314660901 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5092 0.40001131218164854 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4950 0.38885624416715636 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3259 0.25601666661429545 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2471 0.1941138948155643 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1656 0.13009008895773957 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1533 0.12042760046631328 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1353 0.1062873734056894 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1325 0.10408778252959236 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.927840850173296E-5 0.0 16-17 0.0 0.0 0.0 7.855681700346592E-5 0.0 18-19 0.0 0.0 0.0 1.5711363400693185E-4 0.0 20-21 0.0 0.0 0.0 2.3567045101039778E-4 0.0 22-23 0.0 0.0 0.0 5.106193105225285E-4 0.0 24-25 0.0 0.0 0.0 0.001139073846550256 0.0 26-27 0.0 0.0 0.0 0.0019246420165849154 0.0 28-29 0.0 0.0 0.0 0.004752687428709689 0.0 30-31 0.0 0.0 0.0 0.012254863452540683 0.0 32-33 0.0 0.0 0.0 0.02521673825811256 0.0 34-35 3.927840850173296E-5 0.0 0.0 0.04776254473810728 0.0 36-37 7.855681700346592E-5 0.0 0.0 0.08621610666130386 0.0 38-39 7.855681700346592E-5 0.0 0.0 0.14006680471717975 0.0 40-41 7.855681700346592E-5 0.0 0.0 0.20318720717946462 0.0 42-43 7.855681700346592E-5 0.0 0.0 0.2639116267231438 0.0 44-45 7.855681700346592E-5 0.0 0.0 0.3367337960853567 0.0 46-47 7.855681700346592E-5 0.0 0.0 0.4286059935709101 0.0 48-49 7.855681700346592E-5 0.0 0.0 0.5261342818807131 0.0 50-51 1.5711363400693185E-4 0.0 0.0 0.6349354734305133 0.0 52-53 1.5711363400693185E-4 0.0 0.0 0.7434617161208015 0.0 54-55 1.5711363400693185E-4 0.0 0.0 0.8672279813097621 0.0 56-57 1.5711363400693185E-4 0.0 0.0 1.0138542802467314 0.0 58-59 1.5711363400693185E-4 0.0 0.0 1.1803947322940789 0.0 60-61 1.5711363400693185E-4 0.0 0.0 1.3621359284315975 7.855681700346592E-5 62-63 1.5711363400693185E-4 0.0 0.0 1.5527933232990092 7.855681700346592E-5 64-65 1.5711363400693185E-4 0.0 0.0 1.7458859794935284 7.855681700346592E-5 66-67 1.5711363400693185E-4 0.0 0.0 1.975861061271175 7.855681700346592E-5 68-69 1.5711363400693185E-4 0.0 0.0 2.2433077447594747 7.855681700346592E-5 70-71 1.5711363400693185E-4 0.0 0.0 2.5360890017313924 7.855681700346592E-5 72-73 1.5711363400693185E-4 0.0 0.0 2.8313055200304174 7.855681700346592E-5 74-75 1.5711363400693185E-4 0.0 0.0 3.140701543798568 7.855681700346592E-5 76-77 1.5711363400693185E-4 0.0 0.0 3.4876870045028765 7.855681700346592E-5 78-79 1.5711363400693185E-4 0.0 0.0 3.8763075782190226 7.855681700346592E-5 80-81 1.5711363400693185E-4 0.0 0.0 4.295840259426033 7.855681700346592E-5 82-83 1.5711363400693185E-4 0.0 0.0 4.730887911991227 7.855681700346592E-5 84-85 1.5711363400693185E-4 0.0 0.0 5.171984439465688 7.855681700346592E-5 86-87 1.5711363400693185E-4 0.0 0.0 5.638219148381259 7.855681700346592E-5 88-89 1.5711363400693185E-4 0.0 0.0 6.1481707259592575 7.855681700346592E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1565 0.0 30.013016 2 GTATAGG 350 0.0 27.204285 1 GTACCTG 1885 0.0 25.25597 1 GTACATG 7515 0.0 22.869337 1 TACATGG 7610 0.0 22.070255 2 GTACCCG 195 4.1312524E-7 21.972692 1 CAACGGT 110 0.0028970097 21.565825 4 ACCTGGG 2100 0.0 21.463129 3 ACATGGG 7530 0.0 21.233603 3 TGCACCG 380 0.0 21.225313 5 GAGTACT 5760 0.0 20.552797 12-13 CATGGGG 2975 0.0 20.253752 4 GCACCGT 380 0.0 19.976765 6 AGTACTT 5815 0.0 19.86882 12-13 GTAAGGT 240 1.430908E-7 19.768673 4 AATTCGC 235 0.0 19.18057 78-79 TACCCGG 225 1.6865815E-6 18.977926 2 GTACTTT 6220 0.0 18.765821 14-15 GTATCAA 13810 0.0 18.408693 1 ATTCGCG 235 0.0 18.195724 80-81 >>END_MODULE