FastQCFastQC Report
Fri 27 May 2016
SRR939180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255912
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.49207269299122874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48730.3880048920625012No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46140.3673824280682086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31260.24890278936740792No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26370.20996694035887864No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17500.13934097293440942No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16000.12739746096860288No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.10239570925351459No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15250.034.4764982
GTACCTG16850.032.1267661
ACCTGGG20150.025.6224983
GTACATG76050.025.2881971
TACATGG76950.024.6834452
ACATGGG78350.023.1542023
GTATCAA129250.021.4925211
GAGTACT55300.021.06403212-13
AGTACTT56750.021.02646312-13
CATGGGG32950.020.7003214
CATGGGA43400.020.0815894
GTACTTT61600.018.87207414-15
ATGGGGA18650.018.7941595
ATGGGAG16050.018.5924035
TATCAAC149150.018.54642
ATCAACG149500.018.4396133
CAACGCA149350.018.4264185
TCAACGC149650.018.421134
GTACGTG1559.6447725E-418.3815291
GTGTACG1300.00757671718.2636971
Produced by FastQC (version 0.11.5)