FastQCFastQC Report
Fri 27 May 2016
SRR939180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255912
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.4570383912248629No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.3877660218231851No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47960.38187388925338717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.23910910955544656No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23990.19101656803979897No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15390.12254043276917492No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15220.1211868347463835No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT13320.10605838625636192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.10589913943015115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15150.032.890512
GTACCTG18250.026.8884681
TATAACG950.001233215924.9770052
ACCTGGG19950.024.02553
TAACGCA1000.001662612723.7281534
ATAACGC1000.001662612723.7281533
GTACATG76850.022.8756871
TACATGG77150.022.4517862
GAGTACT53900.021.65909412-13
ACATGGG78500.020.97753
AGTACTT54500.020.24512112-13
GTACTTT58150.020.07609714-15
GTATCAA131350.019.4776211
GTATAGG2952.906745E-919.3798731
CATGGGA46750.018.8810124
TGTACCG1801.2421672E-418.455235
ACTTTTT66250.017.62151116-17
TAGACAG6550.017.388575
CATGGGG32350.017.3101834
GGTATCA105800.017.2016221
Produced by FastQC (version 0.11.5)