FastQCFastQC Report
Fri 27 May 2016
SRR939179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404268
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97810.4068182082862643No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT95950.3990819659039674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72870.30308601204191876No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51550.21441037355236603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47630.19810603476817062No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36750.15285317610183224No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36350.15118946806262862No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27440.11413037148936807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG6500.023.3710351
GTACATG110850.022.2694231
TACATGG110700.021.9549752
GAGTACT88450.021.89862412-13
AGTACTT92150.020.94226612-13
ACATGGG112000.020.5157263
GTACTTT93250.020.46839314-15
GTATCAA206950.020.3698981
AGAGTAC148850.020.23483510-11
ATCACGC2001.3148663E-518.950613
TACCGTG2502.2426866E-718.9442987
TATCACG2051.6266296E-518.48842
TACTTTT104950.018.20910814-15
CATGGGG54950.018.1918954
GTACCGT2352.601695E-618.1381576
TACCTGG19650.018.0826422
CAACGCA231350.018.0004165
TCAACGC231650.017.9771044
ATCAACG231400.017.955583
TATCAAC233700.017.7991392
Produced by FastQC (version 0.11.5)