FastQCFastQC Report
Fri 27 May 2016
SRR939179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404268
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT102750.42736500257042886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100410.4176323105410878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79750.331701790316221No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.2158661180866692No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48540.20189097055735883No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34660.14416030159699336No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33900.14099925632250648No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28700.11937105181285947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108450.023.5346451
TACATGG106950.023.425422
ACATGGG107750.022.3267213
GAGTACT93300.021.59113512-13
GTATCAA211650.020.968631
GTACTTT97850.020.53865614-15
AGAGTAC150400.020.33548410-11
AGTACTT97400.020.21941412-13
CATGGGG52700.019.1779984
TCAACGC237500.018.5204014
CAACGCA238150.018.4698525
ATCAACG238300.018.4582273
TATCAAC241400.018.2605022
CATGGGA57800.018.1425654
AACGCAG244100.018.0196446
TACCTGG16750.017.8467862
TACTTTT109400.017.63285614-15
ACTTTTT116300.017.34049616-17
GAGTACA87550.017.1327381
GGTATCA164550.016.6395781
Produced by FastQC (version 0.11.5)