##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939179_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404268 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50941367601283 34.0 31.0 34.0 31.0 34.0 2 32.70804710622942 34.0 31.0 34.0 31.0 34.0 3 32.8008699529337 34.0 31.0 34.0 31.0 34.0 4 36.25539290960908 37.0 35.0 37.0 35.0 37.0 5 36.21638810648397 37.0 37.0 37.0 35.0 37.0 6 36.260535847085265 37.0 37.0 37.0 35.0 37.0 7 36.21503384814006 37.0 37.0 37.0 35.0 37.0 8 36.20415278163666 37.0 37.0 37.0 35.0 37.0 9 37.95007586508659 39.0 38.0 39.0 35.0 39.0 10-11 37.891604014194755 39.0 38.0 39.0 35.0 39.0 12-13 37.82603291313614 39.0 38.0 39.0 35.0 39.0 14-15 39.18560846794118 40.5 39.0 41.0 36.0 41.0 16-17 39.13913257590252 40.0 38.5 41.0 36.0 41.0 18-19 39.17444997812224 40.5 38.5 41.0 36.0 41.0 20-21 39.24133249704276 41.0 39.0 41.0 36.0 41.0 22-23 39.19889421645174 41.0 39.0 41.0 36.0 41.0 24-25 39.138570242585274 41.0 39.0 41.0 35.5 41.0 26-27 39.004615334064255 40.0 38.5 41.0 35.0 41.0 28-29 38.86258395486693 40.0 38.0 41.0 35.0 41.0 30-31 38.67423265625962 40.0 38.0 41.0 35.0 41.0 32-33 38.52598067270371 40.0 38.0 41.0 34.0 41.0 34-35 38.46566272977888 40.0 38.0 41.0 34.0 41.0 36-37 38.35966144373256 40.0 38.0 41.0 34.0 41.0 38-39 38.22610104198034 40.0 38.0 41.0 34.0 41.0 40-41 38.06594231591487 40.0 38.0 41.0 33.5 41.0 42-43 37.89457123748268 40.0 38.0 41.0 33.0 41.0 44-45 37.660838558763 40.0 37.0 41.0 33.0 41.0 46-47 37.35158372527522 40.0 37.0 41.0 32.5 41.0 48-49 37.01589028344594 40.0 37.0 41.0 31.5 41.0 50-51 36.75047810809777 40.0 36.0 41.0 31.0 41.0 52-53 36.52299972382447 39.5 36.0 41.0 31.0 41.0 54-55 36.280001231143956 39.0 35.0 41.0 30.0 41.0 56-57 36.003551600736685 39.0 35.0 40.5 29.5 41.0 58-59 35.704258219133635 38.5 35.0 40.0 29.0 41.0 60-61 35.3341705250829 38.0 35.0 40.0 28.0 41.0 62-63 34.9568748159523 37.5 34.0 40.0 28.0 41.0 64-65 34.55687593895523 37.0 34.0 40.0 27.0 41.0 66-67 34.19424747989825 36.5 34.0 39.0 26.0 41.0 68-69 33.72707576692781 36.0 33.5 39.0 26.0 40.0 70-71 33.29528592486362 35.5 33.0 38.5 25.5 40.0 72-73 32.831967567675484 35.0 33.0 37.5 24.5 40.0 74-75 32.351851790233034 35.0 32.0 37.0 23.5 39.0 76-77 30.38172096455137 33.5 29.5 35.5 20.0 37.5 78-79 31.359058765495362 34.5 31.0 36.0 21.0 38.0 80-81 31.39120514019236 35.0 32.0 36.0 21.0 37.5 82-83 31.108446936863942 35.0 31.5 35.0 20.0 37.0 84-85 30.74095109197477 34.0 31.0 35.0 19.0 36.5 86-87 30.21233073850336 34.0 31.0 35.0 14.0 36.0 88-89 29.676960097626388 34.0 30.0 35.0 7.0 36.0 90-91 29.237413424792912 34.0 29.5 35.0 2.0 35.0 92-93 28.969473037115662 34.0 29.0 35.0 2.0 35.0 94-95 28.655337716094877 34.0 29.0 35.0 2.0 35.0 96-97 28.107110771344956 34.0 29.0 35.0 2.0 35.0 98-99 27.375698341449457 34.0 28.0 35.0 2.0 35.0 100-101 23.78329703676961 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1617703532247603 1101 2 0.19752307718953688 1101 3 0.19664709156784 1101 4 0.15680480832902077 1101 5 0.09907548116773057 1101 6 0.14550254010408992 1101 7 0.1165991987889683 1101 8 0.1388638936638955 1101 9 0.14600100415271555 1101 10-11 0.15092216139705528 1101 12-13 0.18356582572423719 1101 14-15 0.3280129546151116 1101 16-17 0.29979874973248144 1101 18-19 0.24002871246979396 1101 20-21 0.2655513730381358 1101 22-23 0.35882014034408627 1101 24-25 0.3267107137420098 1101 26-27 0.31114084789393104 1101 28-29 0.22245056749287073 1101 30-31 0.2535457615721697 1101 32-33 0.31265824781902296 1101 34-35 0.3159388802576828 1101 36-37 0.35142457963588924 1101 38-39 0.3824385088877804 1101 40-41 0.4450701017704546 1101 42-43 0.46631016190343644 1101 44-45 0.5579379304615841 1101 46-47 0.523426258852929 1101 48-49 0.4618235180313448 1101 50-51 0.3862868790915144 1101 52-53 0.4149306172273626 1101 54-55 0.4193828224123095 1101 56-57 0.4484630657245603 1101 58-59 0.36346836393820325 1101 60-61 0.3311198711317047 1101 62-63 0.35482862982372154 1101 64-65 0.3155629888702194 1101 66-67 0.41597176762881105 1101 68-69 0.21101366583980052 1101 70-71 0.20422853603479751 1101 72-73 0.24374522774130725 1101 74-75 0.2871121703478181 1101 76-77 0.32384714603947273 1101 78-79 0.44924619606293703 1101 80-81 0.27900177356056943 1101 82-83 0.24559760532690333 1101 84-85 0.2366912733482316 1101 86-87 0.2946633878567795 1101 88-89 0.5720025964958637 1101 90-91 0.5992206860178939 1101 92-93 0.5334773809523945 1101 94-95 0.3568013847000877 1101 96-97 0.361691244402639 1101 98-99 0.22923113873988044 1101 100-101 -0.08571896768305365 1102 1 0.15651365173589937 1102 2 0.06418122818391225 1102 3 0.14452404240478955 1102 4 0.07805279161068768 1102 5 0.11974025597422866 1102 6 0.1125600070828483 1102 7 0.1446864176854703 1102 8 0.14243357142625257 1102 9 0.22206851272017758 1102 10-11 0.21359279130364683 1102 12-13 0.15736540362308205 1102 14-15 0.2495714147053789 1102 16-17 0.20460619196975216 1102 18-19 0.23488777809340888 1102 20-21 0.23980734494102052 1102 22-23 0.26805227987222935 1102 24-25 0.264909948252118 1102 26-27 0.2106454429046707 1102 28-29 0.15870627241792334 1102 30-31 0.26159560357192646 1102 32-33 0.26077329225012136 1102 34-35 0.23767237193649038 1102 36-37 0.2900825096831028 1102 38-39 0.36955659824710096 1102 40-41 0.29047313473071057 1102 42-43 0.26158453916613666 1102 44-45 0.25855324532282253 1102 46-47 0.36627021721360364 1102 48-49 0.40582572289999774 1102 50-51 0.5379383441323782 1102 52-53 0.5378550371834976 1102 54-55 0.4980027311335036 1102 56-57 0.5154697838995403 1102 58-59 0.5567845968809664 1102 60-61 0.4517387303538243 1102 62-63 0.5880281290101266 1102 64-65 0.47145318733141295 1102 66-67 0.36656717422915364 1102 68-69 0.3415507515926208 1102 70-71 0.5606346231294097 1102 72-73 0.48990373892561223 1102 74-75 0.38215133093268605 1102 76-77 0.51598579959661 1102 78-79 0.5115753935413778 1102 80-81 0.4559726338506067 1102 82-83 0.3854251744707078 1102 84-85 0.3963536844802853 1102 86-87 0.5092598255439249 1102 88-89 0.5160784504221638 1102 90-91 0.64991689976587 1102 92-93 0.6158913669084427 1102 94-95 0.5554858284080417 1102 96-97 0.57749621709495 1102 98-99 0.6027662704911414 1102 100-101 0.024395004233639384 1103 1 0.13104299389972596 1103 2 0.06530561784409628 1103 3 0.13005527010767537 1103 4 0.06363613445000027 1103 5 0.10287217437042528 1103 6 0.11398590405591591 1103 7 0.10918952334500176 1103 8 0.1427422361827766 1103 9 0.10144769395335373 1103 10-11 0.18493930775346 1103 12-13 0.17156677829959932 1103 14-15 0.2402773623173644 1103 16-17 0.16973479110146883 1103 18-19 0.23417717814307082 1103 20-21 0.25304201738732246 1103 22-23 0.16669929935613226 1103 24-25 0.15585611756591788 1103 26-27 0.19561727165388731 1103 28-29 0.1443632027245485 1103 30-31 0.23962795521713076 1103 32-33 0.23821448378554777 1103 34-35 0.2041065335202532 1103 36-37 0.2270046299863111 1103 38-39 0.2895317932315251 1103 40-41 0.30558585328436294 1103 42-43 0.36290810706231724 1103 44-45 0.3295988136350232 1103 46-47 0.2780768678207508 1103 48-49 0.24674561097548064 1103 50-51 0.19345841069659997 1103 52-53 0.4396125250997258 1103 54-55 0.3562679009997751 1103 56-57 0.17865290038469794 1103 58-59 0.15962744402257556 1103 60-61 0.28485532734725894 1103 62-63 0.27982692879629667 1103 64-65 0.13766613917299964 1103 66-67 0.1043639565109018 1103 68-69 0.08520567781503985 1103 70-71 0.08950104062757447 1103 72-73 0.15648932517616032 1103 74-75 0.007344190487984292 1103 76-77 -0.056386915333593635 1103 78-79 0.08349808420337013 1103 80-81 0.02736198312714322 1103 82-83 0.08512948258076491 1103 84-85 0.09359404262087168 1103 86-87 0.19431978031782648 1103 88-89 0.1030599551404876 1103 90-91 0.1736610479965286 1103 92-93 0.14351004067887274 1103 94-95 -0.01881268619873211 1103 96-97 0.05765980941590598 1103 98-99 0.10820984187657601 1103 100-101 -0.23070433884723585 1104 1 0.11307872681574338 1104 2 0.14130327042624913 1104 3 0.12661891121356916 1104 4 0.045613181920003854 1104 5 0.0658025343078279 1104 6 0.08777307150506175 1104 7 0.11698164645402187 1104 8 0.08765616419529465 1104 9 0.05833997355690457 1104 10-11 0.08260493112330636 1104 12-13 0.08931260511233319 1104 14-15 0.1752049836006151 1104 16-17 0.1265096998129991 1104 18-19 0.20382702164854294 1104 20-21 0.15364517336019645 1104 22-23 0.150104359345697 1104 24-25 0.18868736430562194 1104 26-27 0.22533166914997338 1104 28-29 0.24039541451380586 1104 30-31 0.2291395618942289 1104 32-33 0.15138610611209913 1104 34-35 0.20422390441226668 1104 36-37 0.1581274781866142 1104 38-39 0.22175010309068455 1104 40-41 0.3919903916255265 1104 42-43 0.2710001780065028 1104 44-45 0.29287150534081974 1104 46-47 0.363059914247458 1104 48-49 0.2807244842149146 1104 50-51 0.2552281029240433 1104 52-53 0.1948420503996644 1104 54-55 0.18667348263775096 1104 56-57 0.24080404801119215 1104 58-59 0.29401385508152345 1104 60-61 0.23132115937647058 1104 62-63 0.18763861372065804 1104 64-65 0.31179889869308397 1104 66-67 0.24695002849838232 1104 68-69 0.16288564651613058 1104 70-71 0.269365412041239 1104 72-73 0.21954031630813375 1104 74-75 0.2634507371755106 1104 76-77 0.3100287080362527 1104 78-79 0.37977155838177623 1104 80-81 0.21407625021112864 1104 82-83 0.14272924783898233 1104 84-85 0.17641550845290155 1104 86-87 0.27158569059429993 1104 88-89 0.3328721617132544 1104 90-91 0.3425479807038805 1104 92-93 0.2350071716126223 1104 94-95 0.23615927519928448 1104 96-97 0.22274196904032095 1104 98-99 0.3454751000925391 1104 100-101 0.13095776339585186 1105 1 0.041230221684109836 1105 2 0.07145164168908025 1105 3 0.07152631862097536 1105 4 0.036978643767390906 1105 5 0.0764283709878768 1105 6 0.08275299118377433 1105 7 0.020110829854289136 1105 8 0.03937414723858268 1105 9 0.0015740208572196934 1105 10-11 0.052741031078682 1105 12-13 0.05221142260452183 1105 14-15 0.15497684883934681 1105 16-17 0.12801014604236371 1105 18-19 0.027881573188039965 1105 20-21 0.1041890153950007 1105 22-23 0.09448744725288805 1105 24-25 0.06774247363181018 1105 26-27 0.18458535054221414 1105 28-29 0.1171608094267853 1105 30-31 0.10931861142569232 1105 32-33 0.20029284417546478 1105 34-35 0.298657193122672 1105 36-37 0.29663149425087454 1105 38-39 0.36090115172967785 1105 40-41 0.3304609404876473 1105 42-43 0.3522529669400143 1105 44-45 0.34815095647869754 1105 46-47 0.300559914247458 1105 48-49 0.24599110625864995 1105 50-51 0.37688696057687565 1105 52-53 0.36207765950274506 1105 54-55 0.33208289807728875 1105 56-57 0.2690196883503333 1105 58-59 0.24542226650499543 1105 60-61 0.3242818911926193 1105 62-63 0.2979353562463203 1105 64-65 0.22544793929995421 1105 66-67 0.29048528061797185 1105 68-69 0.16301393745767712 1105 70-71 0.22683975385293564 1105 72-73 0.18371088748172326 1105 74-75 0.20277191075873446 1105 76-77 0.24532823949542504 1105 78-79 0.44390308448619464 1105 80-81 0.24347997622630402 1105 82-83 0.1568775075444684 1105 84-85 0.17349549506602102 1105 86-87 0.25306996100929524 1105 88-89 0.4473076815704644 1105 90-91 0.21239124264051767 1105 92-93 0.12766669860949875 1105 94-95 -0.09012642314966612 1105 96-97 0.012473115849783056 1105 98-99 0.20217411838794064 1105 100-101 -0.059328381182464085 1106 1 0.0498734522363975 1106 2 0.06670955230936215 1106 3 0.12535714988950275 1106 4 0.04266333657837862 1106 5 0.00899290986046708 1106 6 0.0589750342898796 1106 7 -0.023393703754074124 1106 8 0.0695052629661248 1106 9 0.17531899346005986 1106 10-11 0.12157195892942241 1106 12-13 0.14380063325852888 1106 14-15 0.07698709748100185 1106 16-17 0.05271113627350843 1106 18-19 0.021746846261088137 1106 20-21 0.06386467084193725 1106 22-23 0.09630611969809166 1106 24-25 0.11271710567795168 1106 26-27 0.09191149230500884 1106 28-29 0.13054515378083664 1106 30-31 0.0393827439700587 1106 32-33 0.14033167608084085 1106 34-35 -0.005202564032565249 1106 36-37 0.08740953133727913 1106 38-39 0.18153548423045152 1106 40-41 0.07002991453278185 1106 42-43 0.036405893078807594 1106 44-45 -0.03374420908370723 1106 46-47 0.04394847114716782 1106 48-49 -0.08191762334957531 1106 50-51 -0.06508497906919075 1106 52-53 -0.0027000150588847305 1106 54-55 -0.03195333722977267 1106 56-57 -0.12079765808930887 1106 58-59 -0.15278153836614905 1106 60-61 -0.20978841324819086 1106 62-63 -0.08031592805329524 1106 64-65 -0.00754007845634419 1106 66-67 0.14654860499354783 1106 68-69 -0.1255750977874186 1106 70-71 -0.22592519995247784 1106 72-73 -0.11121433154041682 1106 74-75 -0.17036529628251884 1106 76-77 -0.09608424520995484 1106 78-79 0.01216388457329387 1106 80-81 0.06925882401799655 1106 82-83 0.15155904670212195 1106 84-85 0.07119082870100257 1106 86-87 0.0956303107500922 1106 88-89 0.18405274763367885 1106 90-91 0.32004549078501654 1106 92-93 0.31558418500555163 1106 94-95 0.1153600517300788 1106 96-97 0.1269159232743995 1106 98-99 0.13466217194795504 1106 100-101 -0.05057969065198975 1107 1 0.047125186957373444 1107 2 0.019283057507244905 1107 3 0.04625417552406219 1107 4 0.009301060562563634 1107 5 0.10297044779748887 1107 6 0.08617857670814999 1107 7 0.06068419125173108 1107 8 0.0929024117376187 1107 9 0.13460737712793502 1107 10-11 0.0660513576966153 1107 12-13 0.08776436664943787 1107 14-15 0.12461004522244679 1107 16-17 0.1185913747118974 1107 18-19 0.04065775343773481 1107 20-21 0.02479610687895928 1107 22-23 0.09534225477241876 1107 24-25 -0.009333104371947343 1107 26-27 0.08211186864165398 1107 28-29 0.022579732517606033 1107 30-31 0.10384214265799585 1107 32-33 0.20825881827181547 1107 34-35 0.16615384255066346 1107 36-37 0.16786408682806098 1107 38-39 0.17671521602407125 1107 40-41 0.13442373454980583 1107 42-43 0.13233767540894803 1107 44-45 -0.04299353805821937 1107 46-47 0.17238491145010215 1107 48-49 0.14305184718427455 1107 50-51 0.253723323435878 1107 52-53 0.22499318772603516 1107 54-55 0.22174657363984096 1107 56-57 0.3201734233209024 1107 58-59 0.18068713550480453 1107 60-61 0.3550391695748445 1107 62-63 0.15174170040682355 1107 64-65 0.2792458287910762 1107 66-67 0.3844512273666467 1107 68-69 0.2516852197190289 1107 70-71 0.34419557236989107 1107 72-73 0.2906938473748113 1107 74-75 0.2510941018392785 1107 76-77 0.25961220121869033 1107 78-79 0.4437956196679238 1107 80-81 0.433542773812853 1107 82-83 0.46076172518036884 1107 84-85 0.40440898261329394 1107 86-87 0.4496894606353834 1107 88-89 0.5273693563615112 1107 90-91 0.6641992419133018 1107 92-93 0.5317997993720169 1107 94-95 0.48230621249048156 1107 96-97 0.5647664978850919 1107 98-99 0.7273248339437828 1107 100-101 0.25705362889881656 1108 1 -0.03693330588163235 1108 2 -0.03502644126243126 1108 3 -0.021452898346311144 1108 4 0.003634454150905242 1108 5 0.006219970278095843 1108 6 -0.0032059560951864796 1108 7 -0.012052857798842354 1108 8 0.025799420361558134 1108 9 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2316 72-73 0.0727204598024187 2316 74-75 -0.17049099251229194 2316 76-77 -0.15335601176207803 2316 78-79 -0.10337634737738455 2316 80-81 -0.01756157216621901 2316 82-83 -0.11469876263222645 2316 84-85 -0.25612036511700254 2316 86-87 -0.37651155586681995 2316 88-89 -0.7166370005904064 2316 90-91 -0.7062485579814002 2316 92-93 -0.701288155127731 2316 94-95 -0.4200919367456457 2316 96-97 -0.65004380757399 2316 98-99 -0.583691566574128 2316 100-101 -0.06301159863633998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 9.0 5 41.0 6 94.0 7 240.0 8 503.0 9 1039.0 10 1669.0 11 2655.0 12 4223.0 13 6248.0 14 7594.0 15 10222.0 16 13704.0 17 19921.0 18 20026.0 19 13020.0 20 11472.0 21 12055.0 22 12407.0 23 13384.0 24 15558.0 25 18115.0 26 21385.0 27 25640.0 28 31368.0 29 38346.0 30 47070.0 31 58486.0 32 73223.0 33 95811.0 34 134363.0 35 200726.0 36 331549.0 37 574092.0 38 532335.0 39 55658.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46586344208223 18.996631481768222 14.449069264764866 23.08843581138468 2 15.5778390761762 20.613800125443586 39.79157065684857 24.016790141531644 3 20.248990545147212 24.4021049234112 29.281677416993446 26.067227114448137 4 13.298559062467247 16.406698421307443 36.39976907732416 33.894973438901154 5 13.93563446337929 37.32038192081748 33.59118035094257 15.152803264860657 6 32.30176502785879 35.43644052992428 17.036952619258752 15.224841822958174 7 28.63287287440502 32.67356218191982 20.415402941768555 18.27816200190661 8 26.11048352346743 34.90754774426145 19.417344489050308 19.564624243220806 9 26.45478790218062 17.393402066658126 20.550121700243068 35.601688330918186 10-11 25.56667975450324 26.368898974656734 26.438712323251817 21.62570894758821 12-13 26.48153200891082 23.798178905180286 27.29618744665736 22.424101639251532 14-15 22.962522382379426 25.12171231181644 26.296035953224205 25.619729352579927 16-17 22.164472166874184 28.42433369013495 26.844160740827473 22.567033402163396 18-19 22.035879950962194 27.055433723576932 29.15948412562246 21.749202199838415 20-21 22.974289887816166 25.87488166876571 29.11002434004861 22.040804103369506 22-23 22.4546722744719 25.893078475444504 29.185910222986788 22.46633902709681 24-25 22.62058507056442 26.060441957744118 29.256377334037087 22.06259563765438 26-27 22.23395381366956 26.06574760958715 29.607553656780034 22.092744919963256 28-29 21.810567707094215 26.927322578015428 29.325141789517637 21.936967925372713 30-31 22.175750316997274 26.151209102568608 29.123902103838677 22.54913847659544 32-33 22.05895931734732 26.30761212976257 29.206041090261152 22.42738746262896 34-35 22.748665041324756 26.296824426883948 29.02275242958943 21.931758102201865 36-37 22.588891674112226 25.984587429258504 29.277893289426192 22.14862760720307 38-39 22.138789199843824 26.56527401538737 29.2262486819933 22.069688102775505 40-41 22.349267268337844 26.485643942143554 28.95295196524171 22.212136824276893 42-43 22.103340058888996 26.525817543945763 28.5639292109095 22.80691318625574 44-45 22.39121464912835 26.366867095030994 28.60864642639665 22.633271829444002 46-47 22.732101315966244 26.831253605607756 28.199044937674717 22.237600140751283 48-49 22.644007366948223 26.80821311576324 28.15728134987514 22.3904981674134 50-51 22.195339450792883 27.07235805023238 28.305457881948172 22.426844617026568 52-53 22.750015441299873 27.193137699169373 27.641008182745132 22.415838676785622 54-55 22.238972527189148 26.82161472847453 27.94002166147867 22.999391082857652 56-57 22.517435660250854 27.063081154014444 27.74104217998992 22.678441005744784 58-59 22.9074586612028 26.717233226767956 27.797320309230923 22.57798780279832 60-61 22.470285263137754 26.929804419752536 28.347093583846434 22.252816733263284 62-63 22.180914296267396 26.997345441204974 28.26086052294226 22.56087973958537 64-65 21.95872510941169 27.261084874857644 28.00282170282112 22.77736831290954 66-67 22.16582247285183 27.09983809364364 27.578964444033122 23.155374989471408 68-69 22.10366703297343 26.957342592769752 27.950613473711183 22.988376900545635 70-71 22.45324666519983 26.89620162818855 27.72630909961331 22.92424260699831 72-73 22.3815523061489 26.685918541526988 28.034208332848088 22.89832081947603 74-75 21.93068742752472 27.556890496400566 27.46110250604342 23.051319570031296 76-77 22.13370469706061 27.010771396017358 27.565232668864866 23.29029123805717 78-79 22.206599463617852 27.09709365392038 27.664608094978238 23.031698787483528 80-81 22.380755601521592 27.21113405946825 27.472531814762597 22.935578524247557 82-83 22.308663821850956 27.037402207293603 27.713120459485086 22.940813511370354 84-85 22.10677012712393 27.176733209442546 27.483978491582466 23.23251817185106 86-87 22.072290952576058 27.18411795860856 27.506645414663883 23.236945674151503 88-89 21.980124048256208 27.159568888042525 27.21164543513776 23.648661628563506 90-91 22.096051306644597 26.953379859452898 27.3105621612661 23.640006672636407 92-93 22.33230654818847 26.90841869541998 27.42915931169071 23.33011544470084 94-95 22.012770622908928 26.82128198686669 27.54044058316294 23.62550680706144 96-97 21.743046478813195 26.683740473969724 27.871794631836934 23.70141841538015 98-99 21.847029801188153 26.906231633955613 27.209259668260106 24.037478896596127 100-101 21.830258916502025 26.170801705313508 27.821129250285953 24.177810127898514 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 649.0 1 470.0 2 412.5 3 560.0 4 499.5 5 466.5 6 847.0 7 1775.0 8 2905.0 9 3445.0 10 3160.0 11 2793.0 12 2851.0 13 3067.0 14 3263.5 15 3426.0 16 3623.0 17 3850.5 18 4056.0 19 4369.0 20 4861.5 21 5638.0 22 6627.0 23 7898.0 24 9438.5 25 11481.5 26 14291.5 27 17374.0 28 19892.0 29 22562.5 30 26112.0 31 30261.5 32 35208.0 33 39306.0 34 42613.5 35 46271.5 36 50885.0 37 55851.5 38 59724.0 39 62089.5 40 64070.5 41 67879.0 42 72001.5 43 75754.5 44 78557.5 45 81183.5 46 83486.5 47 91310.0 48 108755.0 49 108916.5 50 94962.5 51 89128.5 52 86171.0 53 85116.0 54 83456.5 55 77558.0 56 69670.0 57 62351.0 58 56489.0 59 50433.0 60 42931.5 61 35798.0 62 29675.5 63 22994.5 64 17246.0 65 13297.5 66 9875.5 67 8027.0 68 6195.0 69 4264.0 70 3182.5 71 2360.5 72 1787.5 73 1423.5 74 1151.5 75 895.0 76 597.5 77 316.5 78 177.0 79 116.0 80 67.5 81 34.5 82 18.0 83 12.5 84 8.0 85 5.0 86 4.0 87 3.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34351411739456666 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013725591323429833 16-17 0.0015597262867533903 18-19 2.0796350490045202E-5 20-21 0.0 22-23 0.0 24-25 4.5751971078099443E-4 26-27 0.0011853919779325765 28-29 0.0 30-31 0.002807507316156102 32-33 0.0 34-35 0.010855694955803595 36-37 0.029551614046354233 38-39 0.022605632982679133 40-41 0.014661427095481868 42-43 0.010543749698452917 44-45 0.003493786882327594 46-47 0.001123002926462441 48-49 6.654832156814465E-4 50-51 8.318540196018081E-5 52-53 6.238905147013561E-5 54-55 0.0 56-57 0.0 58-59 0.004575197107809945 60-61 0.010065433637181879 62-63 0.004242455499969221 64-65 0.0019132642450841585 66-67 0.004180066448499086 68-69 0.003743343088208136 70-71 4.1592700980090404E-5 72-73 0.0 74-75 0.0 76-77 0.21555417282931852 78-79 4.991124117610849E-4 80-81 0.0032234343259570065 82-83 4.783160612710396E-4 84-85 0.0 86-87 0.10864013495999615 88-89 0.4041146827225584 90-91 0.3914912979751009 92-93 0.0 94-95 0.0 96-97 2.0796350490045202E-5 98-99 0.0014557445343031641 100-101 7.486686176416273E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2404268.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.57477830009457 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96267866031981 42.21609025628598 2 13.979068064972644 15.537672171055734 3 4.433449375783208 7.391638984915333 4 1.9376448676548368 4.307367357769345 5 1.0128249633532098 2.8143761397578024 6 0.600722214587822 2.0031002337396027 7 0.38359084450863673 1.4922583299059475 8 0.2738965048183992 1.2177390025962658 9 0.2124910015441578 1.062822627142346 >10 1.080845049318861 10.92953236762372 >50 0.07957383190695029 3.0647778514479174 >100 0.03916824123240052 4.0557256632892695 >500 0.0026975866661203838 1.1281313013848115 >1k 0.0010490614812688863 1.3844907067491923 >5k 1.4986592589555516E-4 0.5482409960402189 >10k+ 1.4986592589555516E-4 0.8460360102964745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10275 0.42736500257042886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10041 0.4176323105410878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7975 0.331701790316221 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5190 0.2158661180866692 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4854 0.20189097055735883 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3466 0.14416030159699336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3390 0.14099925632250648 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2870 0.11937105181285947 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.1592700980090404E-5 9 0.0 0.0 0.0 0.0 4.1592700980090404E-5 10-11 2.0796350490045202E-5 0.0 0.0 0.0 4.1592700980090404E-5 12-13 4.1592700980090404E-5 0.0 0.0 0.0 2.0796350490045204E-4 14-15 4.1592700980090404E-5 0.0 0.0 0.0 2.7035255637058764E-4 16-17 4.1592700980090404E-5 0.0 0.0 0.0 3.3274160784072323E-4 18-19 6.238905147013561E-5 0.0 0.0 0.0 4.159270098009041E-4 20-21 8.318540196018081E-5 0.0 0.0 0.0 4.159270098009041E-4 22-23 8.318540196018081E-5 0.0 0.0 1.0398175245022602E-4 4.575197107809945E-4 24-25 2.4955620588054244E-4 0.0 0.0 2.0796350490045204E-4 5.199087622511301E-4 26-27 2.4955620588054244E-4 0.0 0.0 7.278722671515822E-4 5.407051127411753E-4 28-29 2.4955620588054244E-4 0.0 0.0 0.003576972284287775 5.407051127411753E-4 30-31 2.4955620588054244E-4 0.0 0.0 0.010710120502373278 5.407051127411753E-4 32-33 2.4955620588054244E-4 0.0 0.0 0.0231879307964004 5.407051127411753E-4 34-35 2.9114890686063284E-4 0.0 0.0 0.041738275433520725 5.822978137212657E-4 36-37 2.9114890686063284E-4 0.0 0.0 0.07669694060728671 5.822978137212657E-4 38-39 2.9114890686063284E-4 0.0 0.0 0.12887498398681013 6.030941642113109E-4 40-41 3.3274160784072323E-4 0.0 0.0 0.1852122974643426 6.238905147013561E-4 42-43 3.7433430882081363E-4 0.0 0.0 0.24523056497861304 6.654832156814465E-4 44-45 3.7433430882081363E-4 0.0 0.0 0.321137244267278 7.070759166615369E-4 46-47 3.7433430882081363E-4 0.0 0.0 0.41168455430093487 7.070759166615369E-4 48-49 3.7433430882081363E-4 0.0 0.0 0.5063703380821106 7.070759166615369E-4 50-51 3.7433430882081363E-4 0.0 0.0 0.6160710869170991 7.070759166615369E-4 52-53 3.7433430882081363E-4 0.0 0.0 0.7234842371981826 7.070759166615369E-4 54-55 4.159270098009041E-4 0.0 0.0 0.8420234349914402 7.070759166615369E-4 56-57 4.159270098009041E-4 0.0 0.0 0.9873691285663662 7.070759166615369E-4 58-59 4.159270098009041E-4 0.0 0.0 1.1507452580161612 7.070759166615369E-4 60-61 4.159270098009041E-4 0.0 0.0 1.3271191065222345 7.070759166615369E-4 62-63 4.159270098009041E-4 0.0 0.0 1.5095238966704212 7.070759166615369E-4 64-65 4.575197107809945E-4 0.0 0.0 1.7038241992989134 7.070759166615369E-4 66-67 4.991124117610849E-4 0.0 0.0 1.9320017568756893 7.070759166615369E-4 68-69 5.822978137212657E-4 0.0 0.0 2.1916026000429234 7.070759166615369E-4 70-71 5.822978137212657E-4 0.0 0.0 2.4731020002761754 7.486686176416273E-4 72-73 5.822978137212657E-4 0.0 0.0 2.770011496222551 7.902613186217177E-4 74-75 5.822978137212657E-4 0.0 0.0 3.078400577639431 7.902613186217177E-4 76-77 5.822978137212657E-4 0.0 0.0 3.40914573583311 7.902613186217177E-4 78-79 5.822978137212657E-4 0.0 0.0 3.7939822016513967 7.902613186217177E-4 80-81 5.822978137212657E-4 0.0 0.0 4.219745885234092 8.526503700918534E-4 82-83 5.822978137212657E-4 0.0 0.0 4.6422237454393604 9.15039421561989E-4 84-85 5.822978137212657E-4 0.0 0.0 5.080049312306282 9.15039421561989E-4 86-87 5.822978137212657E-4 0.0 0.0 5.54387031728576 9.15039421561989E-4 88-89 5.822978137212657E-4 0.0 0.0 6.050344637120322 9.566321225420794E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10845 0.0 23.534645 1 TACATGG 10695 0.0 23.42542 2 ACATGGG 10775 0.0 22.326721 3 GAGTACT 9330 0.0 21.591135 12-13 GTATCAA 21165 0.0 20.96863 1 GTACTTT 9785 0.0 20.538656 14-15 AGAGTAC 15040 0.0 20.335484 10-11 AGTACTT 9740 0.0 20.219414 12-13 CATGGGG 5270 0.0 19.177998 4 TCAACGC 23750 0.0 18.520401 4 CAACGCA 23815 0.0 18.469852 5 ATCAACG 23830 0.0 18.458227 3 TATCAAC 24140 0.0 18.260502 2 CATGGGA 5780 0.0 18.142565 4 AACGCAG 24410 0.0 18.019644 6 TACCTGG 1675 0.0 17.846786 2 TACTTTT 10940 0.0 17.632856 14-15 ACTTTTT 11630 0.0 17.340496 16-17 GAGTACA 8755 0.0 17.132738 1 GGTATCA 16455 0.0 16.639578 1 >>END_MODULE