FastQCFastQC Report
Fri 27 May 2016
SRR939178_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939178_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365152
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94790.4007776244402051No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94540.3997206099227449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72600.3069570158704388No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50930.21533499749698962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.19165787230588138No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36510.15436640012988595No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35730.15106851483541014No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26990.11411528730500195No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108500.025.6021841
TACATGG107850.025.1222762
ACATGGG108800.023.4672053
GAGTACT85850.021.8118112-13
CATGGGG53600.021.2987334
GTACTTT92750.020.24084314-15
GTATCAA205400.020.2282581
AGTACTT89250.019.76075712-13
AGAGTAC143850.019.13886510-11
CAACGCA228700.017.9367625
TCAACGC229450.017.8991554
ATCAACG229800.017.8925063
TATCAAC232250.017.784592
ATGGGGA30250.017.6947885
AACGCAG232200.017.65786
GTGTAGC12650.017.6424241
CATGGGA59450.017.5296134
GAGTACA87050.017.12821
GTACTAG5000.017.0943811
GTATATG12600.016.9587121
Produced by FastQC (version 0.11.5)