##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939178_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2365152 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17454438446239 33.0 31.0 34.0 31.0 34.0 2 32.2676288881222 34.0 31.0 34.0 30.0 34.0 3 32.33494253223471 34.0 31.0 34.0 30.0 34.0 4 35.76525948437986 37.0 35.0 37.0 35.0 37.0 5 35.80359993776298 37.0 35.0 37.0 35.0 37.0 6 35.893097779762144 37.0 37.0 37.0 35.0 37.0 7 35.82411997199334 37.0 36.0 37.0 35.0 37.0 8 35.82318853080056 37.0 37.0 37.0 35.0 37.0 9 37.511287223823246 39.0 38.0 39.0 35.0 39.0 10-11 37.41861051636428 39.0 38.0 39.0 35.0 39.0 12-13 37.32294161220928 39.0 37.0 39.0 34.5 39.0 14-15 38.62146957151168 40.0 38.0 41.0 34.0 41.0 16-17 38.54172459106222 40.0 38.0 41.0 34.0 41.0 18-19 38.44576712194396 40.0 38.0 41.0 34.0 41.0 20-21 38.431399123608124 40.0 38.0 41.0 34.0 41.0 22-23 38.47549290700978 40.0 38.0 41.0 34.0 41.0 24-25 38.42365015018062 40.0 38.0 41.0 34.0 41.0 26-27 38.310782774214935 40.0 38.0 41.0 34.0 41.0 28-29 38.17533460851565 40.0 38.0 41.0 34.0 41.0 30-31 37.97085028784619 40.0 38.0 41.0 33.0 41.0 32-33 37.768868343345375 40.0 38.0 41.0 33.0 41.0 34-35 37.45028078533642 40.0 37.0 41.0 32.5 41.0 36-37 37.190529403607044 40.0 37.0 41.0 31.0 41.0 38-39 37.01306258540677 40.0 37.0 41.0 31.0 41.0 40-41 36.83426562013773 40.0 36.5 41.0 30.5 41.0 42-43 36.61903357585474 40.0 36.0 41.0 30.0 41.0 44-45 36.33794001400332 40.0 36.0 41.0 30.0 41.0 46-47 36.17366325716064 40.0 36.0 41.0 29.5 41.0 48-49 36.02576663148922 40.0 36.0 41.0 28.0 41.0 50-51 34.88832620482743 38.5 34.0 40.0 26.0 40.5 52-53 35.031340903248505 38.5 35.0 39.5 26.5 40.5 54-55 35.55614438310941 39.0 35.0 41.0 27.0 41.0 56-57 35.45368521769426 39.0 35.0 41.0 27.0 41.0 58-59 35.25334671936518 39.0 35.0 41.0 26.5 41.0 60-61 34.945729703630036 38.5 35.0 40.5 26.0 41.0 62-63 34.58012783110769 38.0 34.5 40.0 26.0 41.0 64-65 34.211979399209866 37.0 34.0 40.0 25.0 41.0 66-67 33.813018782725166 37.0 34.0 40.0 23.5 41.0 68-69 33.40004236514186 36.0 34.0 39.0 22.5 41.0 70-71 32.96123378116924 36.0 33.0 39.0 21.5 40.5 72-73 32.5218785092882 35.0 33.0 38.5 20.5 40.0 74-75 32.089255997077565 35.0 32.5 37.0 19.0 39.5 76-77 31.663990728714264 35.0 32.0 37.0 17.5 39.0 78-79 31.24201298690317 35.0 32.0 36.0 14.0 39.0 80-81 30.793164033432102 35.0 31.0 36.0 8.5 37.5 82-83 30.404200026044837 35.0 31.0 35.5 6.0 37.0 84-85 30.031230973738687 34.5 31.0 35.0 2.0 37.0 86-87 29.644344211281137 34.0 30.0 35.0 2.0 36.0 88-89 29.22948271400739 34.0 30.0 35.0 2.0 36.0 90-91 28.823393168811137 34.0 29.0 35.0 2.0 35.5 92-93 28.41793085602955 34.0 29.0 35.0 2.0 35.0 94-95 27.940110191649417 34.0 29.0 35.0 2.0 35.0 96-97 27.354163072817308 34.0 27.0 35.0 2.0 35.0 98-99 26.662385757870954 34.0 25.5 35.0 2.0 35.0 100-101 23.712506426648268 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18222914145444946 1101 2 0.27813009576443903 1101 3 0.19950677691426222 1101 4 0.26247935844386205 1101 5 0.08223823104964367 1101 6 0.07144285727100197 1101 7 0.06259324760603135 1101 8 0.14863731519263013 1101 9 0.21082069666219638 1101 10-11 0.18789395243632612 1101 12-13 0.16374786854520096 1101 14-15 0.3386134761582298 1101 16-17 0.3244765414665238 1101 18-19 0.37972923091317057 1101 20-21 0.47520254894133984 1101 22-23 0.4505481158305358 1101 24-25 0.49441107104929927 1101 26-27 0.3528332169725914 1101 28-29 0.27746439093042596 1101 30-31 0.33723390699900335 1101 32-33 0.2610282483264754 1101 34-35 0.2651586902089278 1101 36-37 0.2864118270467486 1101 38-39 0.1936740278244713 1101 40-41 0.28435697338024823 1101 42-43 0.2665262350286284 1101 44-45 0.22330439387699386 1101 46-47 0.23885479299196533 1101 48-49 0.15402663179439458 1101 50-51 0.10279571722257685 1101 52-53 0.04227316000233827 1101 54-55 0.024358517777358202 1101 56-57 0.08224888555537291 1101 58-59 0.09985564056295715 1101 60-61 -0.021671576289769234 1101 62-63 -0.14466184431068285 1101 64-65 -0.26589269133303617 1101 66-67 -0.0957697456581812 1101 68-69 -0.16778018048189125 1101 70-71 -0.24679420400014607 1101 72-73 -0.2961545449481875 1101 74-75 -0.24572171516974706 1101 76-77 -0.26252851127778953 1101 78-79 -0.4111629515120043 1101 80-81 -0.47934636092801597 1101 82-83 -0.6008948377513867 1101 84-85 -0.5929511938102081 1101 86-87 -0.6074814276611669 1101 88-89 -0.6812407485278946 1101 90-91 -0.6419792198413852 1101 92-93 -0.7246667406101466 1101 94-95 -0.9010223766561971 1101 96-97 -0.8155938865153942 1101 98-99 -1.012733758905707 1101 100-101 -1.3032071610790261 1102 1 0.28043117557634645 1102 2 0.3104145665923994 1102 3 0.36626030894844064 1102 4 0.255028076664388 1102 5 0.2919106161076286 1102 6 0.25292374983473564 1102 7 0.3310543466443292 1102 8 0.30672639489086606 1102 9 0.3606502049747675 1102 10-11 0.450026394148189 1102 12-13 0.4470261380692975 1102 14-15 0.4693392289423386 1102 16-17 0.4309248963247967 1102 18-19 0.4745103890788016 1102 20-21 0.5402639730622312 1102 22-23 0.5652295407759098 1102 24-25 0.6269521627850239 1102 26-27 0.5005065501881418 1102 28-29 0.5110659762969973 1102 30-31 0.47829369392856336 1102 32-33 0.4709045461539674 1102 34-35 0.5137872480082564 1102 36-37 0.4600262508201851 1102 38-39 0.3122835341347283 1102 40-41 0.4140778852604896 1102 42-43 0.36067283432310404 1102 44-45 0.3642839407636771 1102 46-47 0.5566723521634813 1102 48-49 0.4451956629658085 1102 50-51 0.21939333328354138 1102 52-53 0.31501937178893513 1102 54-55 0.3262804508409687 1102 56-57 0.25113356368237305 1102 58-59 0.25372880534803244 1102 60-61 0.16406498313497053 1102 62-63 0.1343821066206914 1102 64-65 0.0442755624669573 1102 66-67 0.09221868799728838 1102 68-69 0.021444244292709413 1102 70-71 -0.034113921555196214 1102 72-73 -0.07058432534075365 1102 74-75 -0.20002979571013668 1102 76-77 -0.3260337202552499 1102 78-79 -0.4193231430280555 1102 80-81 -0.34719837178765545 1102 82-83 -0.5008296151110336 1102 84-85 -0.5292266129724155 1102 86-87 -0.4380048871995754 1102 88-89 -0.43254004830469484 1102 90-91 -0.4975530318470618 1102 92-93 -0.8008211666214642 1102 94-95 -0.865194093292537 1102 96-97 -0.8938257198752311 1102 98-99 -1.0329726533893115 1102 100-101 -1.3095247761977227 1103 1 0.21357125919329434 1103 2 0.2444769784011882 1103 3 0.3194537885460207 1103 4 0.28837346474308134 1103 5 0.3873514927212298 1103 6 0.2323269558363208 1103 7 0.3343633275230786 1103 8 0.33444950580013 1103 9 0.3200845851085532 1103 10-11 0.3314453124357044 1103 12-13 0.3433557951725348 1103 14-15 0.4874042927951123 1103 16-17 0.45630200426990086 1103 18-19 0.458044214320509 1103 20-21 0.4835017748747461 1103 22-23 0.44312945461718556 1103 24-25 0.49848899330277163 1103 26-27 0.42657568462004747 1103 28-29 0.35885145416018815 1103 30-31 0.4025863887528658 1103 32-33 0.40983861697605306 1103 34-35 0.36763537503723853 1103 36-37 0.2727978330195313 1103 38-39 0.3692795850375248 1103 40-41 0.2890762499268149 1103 42-43 0.2632132550991315 1103 44-45 0.38237353462155 1103 46-47 0.2736187543359492 1103 48-49 0.16876329756311748 1103 50-51 0.12237669586598798 1103 52-53 0.18507599171309863 1103 54-55 0.25419073737012354 1103 56-57 0.30300004008964976 1103 58-59 0.20410600674077983 1103 60-61 0.2316633694811543 1103 62-63 0.19206219423719517 1103 64-65 0.16805023030732258 1103 66-67 0.33249826464211196 1103 68-69 0.1814081454699661 1103 70-71 0.12226789801984239 1103 72-73 0.006476335259286259 1103 74-75 -0.0130959715898058 1103 76-77 0.001299106591883259 1103 78-79 0.23410222688442062 1103 80-81 -0.03799298509198579 1103 82-83 -0.0659404253207505 1103 84-85 -0.2924174480018209 1103 86-87 -0.3138239557508946 1103 88-89 -0.21675613475497357 1103 90-91 -0.10647373031208929 1103 92-93 -0.2037617769093032 1103 94-95 -0.22500067537039925 1103 96-97 -0.31422234903201485 1103 98-99 -0.3340317422000787 1103 100-101 -0.6268537997259678 1104 1 0.2124963057219702 1104 2 0.21765547933176066 1104 3 0.28724849837625754 1104 4 0.17780688643390619 1104 5 0.2860773401071697 1104 6 0.2577204474611534 1104 7 0.28207005585198885 1104 8 0.22950314067690414 1104 9 0.34335952730019415 1104 10-11 0.35414002790578536 1104 12-13 0.38372215916779595 1104 14-15 0.41980167590943296 1104 16-17 0.286826283780357 1104 18-19 0.38767390243737054 1104 20-21 0.3429126372062541 1104 22-23 0.45685366644957526 1104 24-25 0.4550059410214615 1104 26-27 0.3985128568043592 1104 28-29 0.5427279416679482 1104 30-31 0.5157807940317554 1104 32-33 0.4684478324743395 1104 34-35 0.5122341004334388 1104 36-37 0.5008944998741924 1104 38-39 0.2691588924651924 1104 40-41 0.45238931743569566 1104 42-43 0.5181821795816788 1104 44-45 0.532369022749684 1104 46-47 0.6776780854509425 1104 48-49 0.7812570091417044 1104 50-51 0.7530196376064353 1104 52-53 0.8509637589005123 1104 54-55 0.6677174420117069 1104 56-57 0.6742356161992831 1104 58-59 0.6116947496204759 1104 60-61 0.7191895101387615 1104 62-63 0.7208721880333755 1104 64-65 0.7331566540848939 1104 66-67 0.6297244786176321 1104 68-69 0.6449941812265436 1104 70-71 0.6148412441368123 1104 72-73 0.5030785573561758 1104 74-75 0.5026748253195663 1104 76-77 0.46915089160119194 1104 78-79 0.4038921992492064 1104 80-81 0.25075564724685506 1104 82-83 0.2246659702576146 1104 84-85 0.2925356849292058 1104 86-87 0.46356637756364094 1104 88-89 0.2879671183046426 1104 90-91 0.23478293879850654 1104 92-93 0.22582256689533153 1104 94-95 0.12173148863614003 1104 96-97 0.17325167950557585 1104 98-99 0.1271079566324751 1104 100-101 0.02175925086339703 1105 1 0.08778932846853138 1105 2 0.20775473206217043 1105 3 0.0990234963233192 1105 4 0.16702780998247846 1105 5 0.16573288440957867 1105 6 0.18683834087259044 1105 7 0.10751050695099451 1105 8 0.23518217059505986 1105 9 0.21995372980203598 1105 10-11 0.18383646674190857 1105 12-13 0.15025521147666865 1105 14-15 0.18603811967260242 1105 16-17 0.1644720287232886 1105 18-19 0.2694314367214403 1105 20-21 0.19165618413642704 1105 22-23 0.1972885609285413 1105 24-25 0.26237089873092856 1105 26-27 0.2376576300230866 1105 28-29 0.1446727039376725 1105 30-31 0.170575089600149 1105 32-33 0.17997987200024568 1105 34-35 0.2061727312603665 1105 36-37 0.31687249237413084 1105 38-39 0.3020967842342728 1105 40-41 0.3523380760922521 1105 42-43 0.19701030757554605 1105 44-45 0.1976918815348938 1105 46-47 0.16607849056412505 1105 48-49 0.27287368447633753 1105 50-51 0.23971002711729028 1105 52-53 0.2535064463337875 1105 54-55 0.3421253471933241 1105 56-57 0.31343020739081595 1105 58-59 0.3778593679107942 1105 60-61 0.3741215441901673 1105 62-63 0.3611442708078485 1105 64-65 0.2937392506414227 1105 66-67 0.15686093059255768 1105 68-69 0.1942594482454041 1105 70-71 0.25836288210796 1105 72-73 0.366693042889807 1105 74-75 0.20502951833683625 1105 76-77 0.24972899906567037 1105 78-79 0.27746327197797527 1105 80-81 0.13598445407903625 1105 82-83 0.17958738377415884 1105 84-85 0.10812783449261332 1105 86-87 0.2834321427622264 1105 88-89 0.15194040273241782 1105 90-91 0.24592913538789318 1105 92-93 0.1655406300163449 1105 94-95 0.17971074027164846 1105 96-97 0.17375161107429093 1105 98-99 0.18097914158854067 1105 100-101 -0.0581149450690468 1106 1 0.1664297120395588 1106 2 0.18229492821163262 1106 3 0.2534943129072431 1106 4 0.2711869296904297 1106 5 0.19641970746718584 1106 6 0.20912382787823702 1106 7 0.2588437601589533 1106 8 0.21595968389337372 1106 9 0.3284267351184056 1106 10-11 0.34037804558221296 1106 12-13 0.18009459248064985 1106 14-15 0.31243076458530084 1106 16-17 0.24481416127362365 1106 18-19 0.33407202264777425 1106 20-21 0.41938205057305566 1106 22-23 0.4617367403327819 1106 24-25 0.4325768470052722 1106 26-27 0.4193580144770479 1106 28-29 0.43429975550468924 1106 30-31 0.5272411021677215 1106 32-33 0.41421163050376464 1106 34-35 0.48534616686691834 1106 36-37 0.5259146061463866 1106 38-39 0.40887585596416187 1106 40-41 0.4904474734261939 1106 42-43 0.5094351768314169 1106 44-45 0.43603728260924157 1106 46-47 0.4539992517749383 1106 48-49 0.5964289404653087 1106 50-51 0.5458214319510475 1106 52-53 0.40865044283418683 1106 54-55 0.5214321091767999 1106 56-57 0.4555768887954841 1106 58-59 0.505911833276457 1106 60-61 0.6606144120105384 1106 62-63 0.5207792666160529 1106 64-65 0.5207753558552 1106 66-67 0.616119287835005 1106 68-69 0.5556074091777319 1106 70-71 0.5379079713270087 1106 72-73 0.5912875771420332 1106 74-75 0.4982698161075234 1106 76-77 0.588478012372331 1106 78-79 0.4499038945310403 1106 80-81 0.4305828347718901 1106 82-83 0.44523332493439227 1106 84-85 0.5047120220096559 1106 86-87 0.40490658209756347 1106 88-89 0.4512469239173065 1106 90-91 0.4952109113493073 1106 92-93 0.37413209134060565 1106 94-95 0.642642332138319 1106 96-97 0.7419042655513408 1106 98-99 0.7677683395327968 1106 100-101 0.49521074748564686 1107 1 0.0038064425087824816 1107 2 -0.017569776818447735 1107 3 0.14558021939357957 1107 4 0.04154288268917128 1107 5 0.1583332302616398 1107 6 0.12390425148674922 1107 7 0.12430774243856035 1107 8 0.16750012408564885 1107 9 0.23725373856559884 1107 10-11 0.15864411070601392 1107 12-13 0.16371581392412082 1107 14-15 0.13284083958550497 1107 16-17 0.13517797671217835 1107 18-19 0.14122682280975596 1107 20-21 0.16935004293777212 1107 22-23 0.20308043089540462 1107 24-25 0.20548006594994916 1107 26-27 0.307988802089632 1107 28-29 0.2984080789748589 1107 30-31 0.17986775507612407 1107 32-33 0.2593460485246837 1107 34-35 0.24221225615195863 1107 36-37 0.30989517600671945 1107 38-39 0.24842398842400115 1107 40-41 0.30270824394529683 1107 42-43 0.29434497148888994 1107 44-45 0.226071108358461 1107 46-47 0.34935374988117474 1107 48-49 0.30950156107388693 1107 50-51 0.2478154975949991 1107 52-53 0.3000924841306585 1107 54-55 0.22796012157689916 1107 56-57 0.1278385703783087 1107 58-59 0.24248468044692117 1107 60-61 0.1541199725093847 1107 62-63 0.2881416528484735 1107 64-65 0.2707764595667612 1107 66-67 0.19285674054096802 1107 68-69 0.14404249869676988 1107 70-71 0.12499332194406065 1107 72-73 0.20661951538394874 1107 74-75 0.0856001906911743 1107 76-77 0.06926219341634621 1107 78-79 0.14131447001427588 1107 80-81 0.16592785299918233 1107 82-83 0.11608356010522769 1107 84-85 0.0855549807111764 1107 86-87 0.036467194123684266 1107 88-89 0.041042689116519426 1107 90-91 0.042900481836941395 1107 92-93 0.03685288883843185 1107 94-95 0.052956536745572436 1107 96-97 0.14407739334548708 1107 98-99 0.13169234317223655 1107 100-101 0.282544292731604 1108 1 -0.021639345915154706 1108 2 -0.024962695003999613 1108 3 -0.02739724868513349 1108 4 0.07489539179670146 1108 5 0.019489413462963512 1108 6 0.1387007195874972 1108 7 0.038905571168740494 1108 8 0.1293188766783402 1108 9 0.1557365946115965 1108 10-11 0.08063505092592038 1108 12-13 0.14143393158992268 1108 14-15 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2316 58-59 -1.0096323001183336 2316 60-61 -0.9434109138146098 2316 62-63 -1.1388159034194274 2316 64-65 -1.337833049904816 2316 66-67 -1.4154967341918194 2316 68-69 -1.4546512120659045 2316 70-71 -1.4298254225298521 2316 72-73 -1.5615047570506633 2316 74-75 -1.2534704544139572 2316 76-77 -1.3348630382950937 2316 78-79 -1.1238914331226617 2316 80-81 -1.152817901091396 2316 82-83 -1.084268316970789 2316 84-85 -1.005123562553571 2316 86-87 -1.0556733074995748 2316 88-89 -1.1940543821874492 2316 90-91 -1.4294106413362506 2316 92-93 -1.2438337280550797 2316 94-95 -1.0410372296619919 2316 96-97 -1.2932448758733344 2316 98-99 -1.5450740404907002 2316 100-101 -1.3755635593417672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6975.0 3 1926.0 4 1873.0 5 2135.0 6 2316.0 7 2922.0 8 3525.0 9 4336.0 10 5416.0 11 6555.0 12 8937.0 13 14395.0 14 12101.0 15 12277.0 16 15068.0 17 21792.0 18 18462.0 19 12803.0 20 12724.0 21 13383.0 22 14107.0 23 15356.0 24 17252.0 25 19487.0 26 22479.0 27 26316.0 28 30696.0 29 36426.0 30 44171.0 31 54330.0 32 68511.0 33 90506.0 34 127257.0 35 191784.0 36 314441.0 37 510424.0 38 507326.0 39 94316.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.29197694990305 18.461215868843997 14.040465008879789 23.206342172373162 2 15.347120570882392 20.248241390666525 40.39006458746229 24.01457345098879 3 20.18777057262024 24.272049327132166 29.133985593165452 26.40619450708214 4 13.064040601778784 16.019464332888525 36.66352816858476 34.25296689674793 5 13.989653757784131 37.19985927281641 33.58358555421066 15.226901415188799 6 32.29375180615126 35.05192195649497 17.35318396700418 15.301142270349585 7 28.76238627184061 32.555546481234565 20.55225095114635 18.129816295778483 8 26.10243978604322 34.53182249592833 19.734259277673708 19.631478440354744 9 26.55969669273214 17.383932500592397 20.716885007278023 35.339485799397444 10-11 25.562446949944444 26.171417158979644 26.619830290597946 21.64630560047797 12-13 26.511488857702687 23.689751016037622 27.334538043254035 22.464222083005655 14-15 23.029671940084484 25.003718168930323 26.38046673968311 25.586143151302075 16-17 22.2964461781468 28.23532174551049 26.891124140676997 22.57710793566571 18-19 22.140167295849256 27.053208069504752 29.01032491381094 21.796299720835048 20-21 23.109889200784462 25.83500359795417 28.93711503953306 22.117992161728303 22-23 22.54956022874961 25.868338205103576 29.057319864761293 22.524781701385525 24-25 22.697894846888914 25.99855838681538 29.09400579269467 22.20954097360104 26-27 22.267939217888067 26.089296424325205 29.43672720300448 22.206037154782248 28-29 21.892011427362185 26.909914294783622 29.162628293302294 22.0354459845519 30-31 22.246972279442183 26.209104480296407 28.938586495948403 22.605336744313007 32-33 22.11145178047351 26.308096293051825 29.109244835473625 22.471207091001038 34-35 22.849953885614262 26.29088462409776 28.82846975753251 22.030691732755468 36-37 22.634118827439593 25.998579067578447 29.08826761644856 22.279034488533398 38-39 22.224800365643066 26.512607015085756 29.0795666764197 22.183025942851483 40-41 22.410360237211094 26.49789051047289 28.868365771740084 22.223383480575936 42-43 22.135382082566885 26.487961360080625 28.47084741664278 22.905809140709714 44-45 22.413996815814503 26.39246488768223 28.497992671103216 22.695545625400044 46-47 22.80449958902235 26.855816970019458 28.054664702587335 22.285018738370862 48-49 22.69075127433867 26.77996199404196 28.060739828348396 22.46854690327097 50-51 22.205910791966218 27.079328378984325 28.252667549297332 22.462093279752125 52-53 22.73939439789685 27.21375813189842 27.606346094956812 22.44050137524792 54-55 22.222918737619437 26.873079831006002 27.913865081048183 22.990136350326374 56-57 22.524124789289814 27.019147939527905 27.737925921362482 22.7188013498198 58-59 22.88080959217699 26.649805119342496 27.822700128915006 22.646685159565507 60-61 22.59885326413373 26.862543493015963 28.335462383118987 22.20314085973132 62-63 22.18746507583139 26.971614060688058 28.350499912251365 22.490420951229183 64-65 21.997604467693254 27.21649431953664 28.05663079177103 22.729270420999075 66-67 22.245776581119994 27.03642712067871 27.590396169485963 23.127400128715337 68-69 22.12697426753423 26.888961022810832 28.052024855167062 22.932039854487883 70-71 22.506544886570886 26.849786031736855 27.763498111144457 22.8801709705478 72-73 22.375298295370012 26.672643200905917 28.12333424299075 22.82872426073332 74-75 22.02617029651791 27.46829034389004 27.64542572429114 22.86011363530091 76-77 22.266524947057015 26.887917269973734 27.606934627792658 23.238623155176597 78-79 22.278122391629335 27.01747811491372 27.77444600304937 22.92995349040757 80-81 22.49193265859929 27.125791464137748 27.572854641687673 22.809421235575293 82-83 22.440952996339156 26.951806867953188 27.84068055261328 22.76655958309438 84-85 22.275111106672817 27.045819272144794 27.6773908836542 23.001678737528188 86-87 22.237923984771626 27.07098598994832 27.675620708308845 23.015469316971206 88-89 22.13562595171018 27.000689757462915 27.438024888217218 23.42565940260969 90-91 22.246460562373887 26.782130807974113 27.6099726128482 23.3614360168038 92-93 22.580500493317786 26.650425367033957 27.727158918892265 23.04191522075599 94-95 22.338460701395384 26.532014984661252 27.84954893415157 23.27997537979179 96-97 22.0395957534989 26.42277977917344 28.239742404437784 23.297882062889876 98-99 22.234027494689755 26.60088492076818 27.580618628394067 23.584468956148005 100-101 22.363518142333813 25.799150892879908 28.170552387104454 23.66677857768183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2353.0 1 1379.0 2 516.5 3 666.5 4 588.5 5 562.5 6 986.0 7 1872.0 8 3011.0 9 3555.0 10 3259.5 11 3036.5 12 3200.5 13 3519.5 14 3792.0 15 4076.0 16 4337.5 17 4474.5 18 4660.0 19 4871.0 20 5089.5 21 5707.5 22 6649.5 23 7900.0 24 9347.5 25 11180.0 26 13922.5 27 16889.0 28 19432.0 29 22178.0 30 25434.0 31 29530.0 32 34555.0 33 38416.0 34 41685.0 35 45580.5 36 50011.5 37 54704.0 38 58639.5 39 60888.0 40 62886.5 41 66712.0 42 70572.0 43 74294.5 44 76915.0 45 79722.0 46 82162.5 47 89032.5 48 106489.0 49 107354.0 50 93414.5 51 86949.5 52 83274.0 53 82310.0 54 80953.0 55 75155.5 56 67826.0 57 60759.0 58 54899.0 59 49053.5 60 41953.0 61 35423.5 62 29138.0 63 22733.5 64 17376.5 65 13430.5 66 10180.0 67 8329.5 68 6518.5 69 4565.5 70 3374.0 71 2486.0 72 1916.0 73 1502.5 74 1189.5 75 961.0 76 692.5 77 426.5 78 282.0 79 191.0 80 117.0 81 77.0 82 61.0 83 51.0 84 40.0 85 24.0 86 14.5 87 13.0 88 10.5 89 12.0 90 8.5 91 3.5 92 6.5 93 7.0 94 5.0 95 6.5 96 5.0 97 4.0 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39067256565328573 2 0.11728633085738252 3 0.11842790653623952 4 0.13056243319668248 5 0.13254962048950766 6 0.07191926776799124 7 0.08248941294259311 8 0.07910696648672051 9 0.07914924706741892 10-11 0.07686609570970492 12-13 0.08149581929618055 14-15 0.07430812057745126 16-17 0.07648557048341925 18-19 0.08595642055986254 20-21 0.08470914342925952 22-23 0.09382060856976634 24-25 0.10658934394068542 26-27 0.10777320020024084 28-29 0.10155795483757493 30-31 0.09278473434265536 32-33 0.09119921256646507 34-35 0.3369762281663081 36-37 0.5273022621801897 38-39 0.5089524901570808 40-41 0.48130099038032226 42-43 0.5091216124798744 44-45 0.5189518474922542 46-47 0.5169223796187307 48-49 0.5227148191744125 50-51 0.5216155240762539 52-53 0.5201991246228572 54-55 0.5145123865189214 56-57 0.4864169406448296 58-59 0.47673468766489424 60-61 0.42417992585677367 62-63 0.5005597948884469 64-65 0.5259281433074915 66-67 0.5358640797716172 68-69 0.5515713155010756 70-71 0.5468570307532031 72-73 0.5334963672525064 74-75 0.5375553029995536 76-77 0.5136667749049533 78-79 0.48840412793765475 80-81 0.4939006034284477 82-83 0.5075995115747318 84-85 0.5029486476979069 86-87 0.5036885578601291 88-89 0.51375133606635 90-91 0.5322702304122526 92-93 0.5375130224188551 94-95 0.5332849643490144 96-97 0.533876892478792 98-99 0.5337923313173952 100-101 0.511256781805144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2365152.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.2293505949151 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36693538827322 44.276594697896314 2 13.188025466619873 15.094842661677148 3 4.194850625088434 7.202057313494542 4 1.8243857878276624 4.176336554878787 5 0.9304013167456993 2.6623131575005137 6 0.5605914506091514 1.9249370800453893 7 0.3744829703075964 1.500199203969103 8 0.2483098448151786 1.1368488932077454 9 0.19019157960036204 0.979608653023484 >10 1.007003355266675 10.516928518194266 >50 0.07409729807047839 2.9205583253241563 >100 0.036731252810360104 3.809305419755486 >500 0.002588485903526038 1.0961741929312299 >1k 0.001109351101510964 1.3791165644413568 >5k 2.958269604029238E-4 1.3241787636604556 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9479 0.4007776244402051 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9454 0.3997206099227449 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7260 0.3069570158704388 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5093 0.21533499749698962 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4533 0.19165787230588138 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3651 0.15436640012988595 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3573 0.15106851483541014 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2699 0.11411528730500195 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.45611613968151E-5 2 0.0 0.0 0.0 0.0 8.45611613968151E-5 3 0.0 0.0 0.0 0.0 8.45611613968151E-5 4 0.0 0.0 0.0 0.0 8.45611613968151E-5 5 4.228058069840755E-5 0.0 0.0 0.0 8.45611613968151E-5 6 4.228058069840755E-5 0.0 0.0 0.0 8.45611613968151E-5 7 4.228058069840755E-5 0.0 0.0 0.0 8.45611613968151E-5 8 4.228058069840755E-5 0.0 0.0 0.0 8.45611613968151E-5 9 4.228058069840755E-5 0.0 0.0 0.0 8.45611613968151E-5 10-11 8.45611613968151E-5 0.0 0.0 4.228058069840755E-5 8.45611613968151E-5 12-13 8.45611613968151E-5 0.0 0.0 4.228058069840755E-5 1.479820324444264E-4 14-15 8.45611613968151E-5 0.0 0.0 4.228058069840755E-5 2.5368348419044527E-4 16-17 1.0570145174601886E-4 0.0 0.0 8.45611613968151E-5 3.382446455872604E-4 18-19 1.2684174209522263E-4 0.0 0.0 1.2684174209522263E-4 3.382446455872604E-4 20-21 1.691223227936302E-4 0.0 0.0 1.2684174209522263E-4 4.2280580698407543E-4 22-23 2.1140290349203772E-4 0.0 0.0 2.1140290349203772E-4 4.2280580698407543E-4 24-25 2.5368348419044527E-4 0.0 0.0 4.8622667803168676E-4 4.2280580698407543E-4 26-27 2.5368348419044527E-4 0.0 0.0 0.0011204353885077999 4.2280580698407543E-4 28-29 2.7482377453964905E-4 0.0 0.0 0.0033613061655233993 4.2280580698407543E-4 30-31 2.959640648888528E-4 0.0 0.0 0.011014091271935166 4.2280580698407543E-4 32-33 2.959640648888528E-4 0.0 0.0 0.02431133390158434 4.2280580698407543E-4 34-35 3.382446455872604E-4 0.0 0.0 0.044436890314026325 4.65086387682483E-4 36-37 3.382446455872604E-4 0.0 0.0 0.0801639810041807 4.65086387682483E-4 38-39 3.805252262856679E-4 0.0 0.0 0.13377575732976146 4.65086387682483E-4 40-41 4.2280580698407543E-4 0.0 0.0 0.19098138301470688 4.65086387682483E-4 42-43 4.2280580698407543E-4 0.0 0.0 0.25034331831527107 4.65086387682483E-4 44-45 4.2280580698407543E-4 0.0 0.0 0.32323503943932563 4.65086387682483E-4 46-47 4.2280580698407543E-4 0.0 0.0 0.4136943418435686 4.8622667803168676E-4 48-49 4.2280580698407543E-4 0.0 0.0 0.5085719649307952 6.342087104761132E-4 50-51 4.2280580698407543E-4 0.0 0.0 0.6181632301010676 6.764892911745208E-4 52-53 4.65086387682483E-4 0.0 0.0 0.7256193259460703 7.399101622221321E-4 54-55 4.65086387682483E-4 0.0 0.0 0.8466263479049126 8.033310332697433E-4 56-57 4.65086387682483E-4 0.0 0.0 0.9932976823476884 8.033310332697433E-4 58-59 4.65086387682483E-4 0.0 0.0 1.1538793278402402 8.033310332697433E-4 60-61 4.65086387682483E-4 0.0 0.0 1.3294071586096792 8.033310332697433E-4 62-63 4.65086387682483E-4 0.0 0.0 1.5099029576111809 8.033310332697433E-4 64-65 5.073669683808905E-4 0.0 0.0 1.7070573054078553 8.033310332697433E-4 66-67 5.073669683808905E-4 0.0 0.0 1.9296222822042726 8.244713236189471E-4 68-69 5.073669683808905E-4 0.0 0.0 2.1889079433372567 8.456116139681509E-4 70-71 5.073669683808905E-4 0.0 0.0 2.476352471215381 8.456116139681509E-4 72-73 5.073669683808905E-4 0.0 0.0 2.767729093098456 8.878921946665584E-4 74-75 5.073669683808905E-4 0.0 0.0 3.0754471594214667 8.878921946665584E-4 76-77 5.073669683808905E-4 0.0 0.0 3.4074131387750133 9.30172775364966E-4 78-79 5.073669683808905E-4 0.0 0.0 3.7959716753933783 9.724533560633735E-4 80-81 5.073669683808905E-4 0.0 0.0 4.220109320669454 0.001014733936761781 82-83 5.073669683808905E-4 0.0 0.0 4.653273869924639 0.0010570145174601886 84-85 5.073669683808905E-4 0.0 0.0 5.089905426797094 0.0010570145174601886 86-87 5.073669683808905E-4 0.0 0.0 5.541039222849102 0.0010570145174601886 88-89 5.073669683808905E-4 0.0 0.0 6.046080759291581 0.0010992950981585962 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10850 0.0 25.602184 1 TACATGG 10785 0.0 25.122276 2 ACATGGG 10880 0.0 23.467205 3 GAGTACT 8585 0.0 21.81181 12-13 CATGGGG 5360 0.0 21.298733 4 GTACTTT 9275 0.0 20.240843 14-15 GTATCAA 20540 0.0 20.228258 1 AGTACTT 8925 0.0 19.760757 12-13 AGAGTAC 14385 0.0 19.138865 10-11 CAACGCA 22870 0.0 17.936762 5 TCAACGC 22945 0.0 17.899155 4 ATCAACG 22980 0.0 17.892506 3 TATCAAC 23225 0.0 17.78459 2 ATGGGGA 3025 0.0 17.694788 5 AACGCAG 23220 0.0 17.6578 6 GTGTAGC 1265 0.0 17.642424 1 CATGGGA 5945 0.0 17.529613 4 GAGTACA 8705 0.0 17.1282 1 GTACTAG 500 0.0 17.094381 1 GTATATG 1260 0.0 16.958712 1 >>END_MODULE