FastQCFastQC Report
Fri 27 May 2016
SRR939178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365152
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103080.43582822583918496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95230.40263796999093504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76470.3233196006007225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.206244672646832No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47170.1994374991543884No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33840.14307748508341114No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32030.13542469997699938No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28210.11927351815020769No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108050.023.9937481
TACATGG110250.022.8990172
ACATGGG111450.021.9286063
GAGTACT95700.020.85461412-13
AGTACTT100150.020.35449412-13
CATGGGG52050.020.3314324
GTATCAA212850.019.9269261
GTACTTT101950.019.57613214-15
AGAGTAC157650.019.49347910-11
GTACGCT1250.006065953518.9820823
ATGGGGA28600.018.4179275
GAGTACA92900.018.2624171
AGTACAT86850.017.9766982
GTAAGCG4000.017.79683194-95
TACTTTT114150.017.79573614-15
TGCACCG6500.017.5219215
CAACGCA241500.017.4886675
TCAACGC242350.017.427334
ATCAACG242500.017.3578413
TATCAAC243600.017.3573842
Produced by FastQC (version 0.11.5)