##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939178_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2365152 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.511229722233494 34.0 31.0 34.0 31.0 34.0 2 32.71786337622275 34.0 31.0 34.0 31.0 34.0 3 32.82572621125408 34.0 31.0 34.0 31.0 34.0 4 36.26908968218533 37.0 37.0 37.0 35.0 37.0 5 36.232572367441925 37.0 37.0 37.0 35.0 37.0 6 36.27829120496273 37.0 37.0 37.0 35.0 37.0 7 36.235208984454275 37.0 37.0 37.0 35.0 37.0 8 36.21125280743056 37.0 37.0 37.0 35.0 37.0 9 37.95724672240939 39.0 38.0 39.0 35.0 39.0 10-11 37.87689036476303 39.0 38.0 39.0 35.0 39.0 12-13 37.79469353343887 39.0 38.0 39.0 35.0 39.0 14-15 39.20372496144011 40.5 38.5 41.0 36.0 41.0 16-17 39.30530617059706 41.0 39.0 41.0 36.0 41.0 18-19 39.34125798257364 41.0 39.0 41.0 36.0 41.0 20-21 39.3100297570727 41.0 39.0 41.0 36.0 41.0 22-23 39.23568083573487 41.0 39.0 41.0 36.0 41.0 24-25 39.16024466926439 41.0 39.0 41.0 36.0 41.0 26-27 39.017160207885155 40.5 39.0 41.0 35.0 41.0 28-29 38.86845898276306 40.0 38.0 41.0 35.0 41.0 30-31 38.680447599139505 40.0 38.0 41.0 35.0 41.0 32-33 38.5759156282556 40.0 38.0 41.0 35.0 41.0 34-35 38.4604321413592 40.0 38.0 41.0 34.0 41.0 36-37 38.30842098097712 40.0 38.0 41.0 34.0 41.0 38-39 38.17712540251113 40.0 38.0 41.0 34.0 41.0 40-41 37.99769866799259 40.0 38.0 41.0 33.0 41.0 42-43 37.795349728051306 40.0 37.5 41.0 33.0 41.0 44-45 37.519430463665756 40.0 37.0 41.0 33.0 41.0 46-47 37.18982606614712 40.0 37.0 41.0 32.0 41.0 48-49 36.82175331648875 40.0 36.5 41.0 31.0 41.0 50-51 36.542805282704876 39.5 36.0 41.0 30.5 41.0 52-53 36.275023338880544 39.0 35.5 41.0 30.0 41.0 54-55 36.02131173810393 39.0 35.0 40.0 30.0 41.0 56-57 35.76442909377494 39.0 35.0 40.0 29.0 41.0 58-59 35.42413151459188 38.0 35.0 40.0 28.0 41.0 60-61 35.031575349068476 38.0 34.0 40.0 27.5 41.0 62-63 34.63227543092368 37.5 34.0 40.0 26.5 41.0 64-65 34.176179163115094 37.0 34.0 39.5 26.0 41.0 66-67 33.742769597894764 36.0 33.0 39.0 26.0 40.5 68-69 33.23882101446334 35.5 33.0 39.0 24.5 40.0 70-71 32.71978608563001 35.0 32.0 38.0 23.5 40.0 72-73 32.14410384617987 35.0 31.5 37.0 21.5 39.5 74-75 31.602743925126163 35.0 31.0 37.0 20.0 39.0 76-77 29.617018906184466 32.5 28.5 35.0 17.5 37.0 78-79 30.60690919653367 34.0 30.5 35.5 18.0 37.5 80-81 30.621197073169082 34.0 31.0 35.0 18.0 37.0 82-83 30.236652232076416 34.0 30.0 35.0 13.0 37.0 84-85 29.757133579575434 34.0 30.0 35.0 7.0 36.0 86-87 29.061119327637293 34.0 29.0 35.0 2.0 36.0 88-89 28.38175791661593 33.5 28.5 35.0 2.0 35.0 90-91 27.815819448390634 33.0 27.0 35.0 2.0 35.0 92-93 27.362002738090403 33.0 26.0 35.0 2.0 35.0 94-95 26.813570967109094 32.5 25.5 34.0 2.0 35.0 96-97 25.987813468225298 32.0 24.5 34.0 2.0 35.0 98-99 24.76776883684431 32.0 11.0 34.0 2.0 35.0 100-101 18.902845144836355 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.176842974496644 1101 2 0.17519292896772498 1101 3 0.20144752883614103 1101 4 0.1340976077172158 1101 5 0.05776092886322459 1101 6 0.059106054784479056 1101 7 0.10370020343654573 1101 8 0.1235577854084795 1101 9 0.1647419060395805 1101 10-11 0.17303912484670292 1101 12-13 0.22764930044365883 1101 14-15 0.28739699495438487 1101 16-17 0.2752735133682407 1101 18-19 0.2614885791878123 1101 20-21 0.24788905954976315 1101 22-23 0.23798567729785702 1101 24-25 0.2587183129307178 1101 26-27 0.2595593611746594 1101 28-29 0.25848927132440025 1101 30-31 0.34124107676374393 1101 32-33 0.4028671825378609 1101 34-35 0.414344600714891 1101 36-37 0.35330980190138206 1101 38-39 0.21319370282180472 1101 40-41 0.4044488746057695 1101 42-43 0.3906023059538555 1101 44-45 0.4596732132007588 1101 46-47 0.5263348059355195 1101 48-49 0.530481112927653 1101 50-51 0.4106630359209973 1101 52-53 0.34277243929297185 1101 54-55 0.2783768099371784 1101 56-57 0.38796982185473894 1101 58-59 0.46184733164813707 1101 60-61 0.5264310630582045 1101 62-63 0.24937146609805438 1101 64-65 0.22952857722652453 1101 66-67 0.1823564525279977 1101 68-69 0.32465067721965113 1101 70-71 0.30954755110099796 1101 72-73 0.3832209109567728 1101 74-75 0.2968009615302165 1101 76-77 0.354045048034461 1101 78-79 0.3696282020449466 1101 80-81 0.48271091669911215 1101 82-83 0.4035161687188271 1101 84-85 0.45326818258038415 1101 86-87 0.5322879323394041 1101 88-89 0.5640388675588248 1101 90-91 0.7002600525756151 1101 92-93 0.6928761518567512 1101 94-95 0.2559009670990733 1101 96-97 0.28128526379857277 1101 98-99 0.3185544710825283 1101 100-101 -0.4465772367168981 1102 1 0.08293856762515617 1102 2 0.12989484825066455 1102 3 0.07638642862000466 1102 4 0.07158104440561885 1102 5 0.13038626528248187 1102 6 0.1438704582402437 1102 7 0.19324072981122242 1102 8 0.23798450342876976 1102 9 0.24651313505202666 1102 10-11 0.2233952728535087 1102 12-13 0.1659086362622233 1102 14-15 0.2195131825364669 1102 16-17 0.22323747749137368 1102 18-19 0.1926087596322077 1102 20-21 0.2632156438857294 1102 22-23 0.2116772492959953 1102 24-25 0.25029016384029745 1102 26-27 0.2561532081620541 1102 28-29 0.18451899400303517 1102 30-31 0.22315791568884435 1102 32-33 0.12824968461379882 1102 34-35 0.15626170465298372 1102 36-37 0.12545458144002453 1102 38-39 0.11415246091205944 1102 40-41 0.11707983518950726 1102 42-43 0.09989042218266064 1102 44-45 0.04207311190690888 1102 46-47 0.06547427932527228 1102 48-49 -0.1340867981222047 1102 50-51 -0.2767512029322603 1102 52-53 -0.21841464220734963 1102 54-55 -0.1979405990602956 1102 56-57 -0.17903061394439135 1102 58-59 -0.3952296929038397 1102 60-61 -0.5564083155157888 1102 62-63 -0.4408931346451581 1102 64-65 -0.33021786423800137 1102 66-67 -0.3000643527581275 1102 68-69 -0.31815966770948023 1102 70-71 -0.38592308251559615 1102 72-73 -0.3907509858883955 1102 74-75 -0.4456925456516352 1102 76-77 -0.5008463555332021 1102 78-79 -0.2387540114612925 1102 80-81 -0.08774278553229209 1102 82-83 -0.43921408339685897 1102 84-85 -0.5287094621019826 1102 86-87 -0.3446238418930889 1102 88-89 -0.3932793188100838 1102 90-91 -0.001618742263111983 1102 92-93 -0.14765890936124748 1102 94-95 -0.0474571891172495 1102 96-97 -0.16008134927657736 1102 98-99 -0.0349802132281134 1102 100-101 -0.44616793888352646 1103 1 0.015411902341512018 1103 2 0.053028091500472385 1103 3 0.09886946320123968 1103 4 0.01984095592938928 1103 5 0.08400166096782158 1103 6 0.07111726653615591 1103 7 0.08344769764094195 1103 8 0.03959516692569309 1103 9 0.12737487179507667 1103 10-11 0.0579506676791226 1103 12-13 0.10656658239526706 1103 14-15 -0.039644452415942055 1103 16-17 0.10196230036137166 1103 18-19 0.22882392468876844 1103 20-21 0.22108711226584887 1103 22-23 0.09861868939886875 1103 24-25 0.1511508633145624 1103 26-27 0.16785897332065502 1103 28-29 0.0891082542528423 1103 30-31 0.18327843510820685 1103 32-33 0.1870088588357035 1103 34-35 0.25721038507888494 1103 36-37 0.18118908922328103 1103 38-39 0.17003975558423434 1103 40-41 0.3288501726663071 1103 42-43 0.32815076356557427 1103 44-45 0.34072335421662814 1103 46-47 0.2277890894676986 1103 48-49 0.17637919721662598 1103 50-51 0.04617393242664747 1103 52-53 0.03130501487719073 1103 54-55 -0.051763027988698695 1103 56-57 -0.029041780937191675 1103 58-59 0.02729660793401223 1103 60-61 0.10946194365188688 1103 62-63 -0.03155836969400383 1103 64-65 0.021801959744031763 1103 66-67 -0.03844273719797542 1103 68-69 -0.13576132472300628 1103 70-71 -0.35893914242674896 1103 72-73 -0.35759109137208966 1103 74-75 -0.416923054810443 1103 76-77 -0.2814231237026945 1103 78-79 -0.17224569181803773 1103 80-81 -0.18123995126541814 1103 82-83 -0.204151454789546 1103 84-85 -0.204579319444818 1103 86-87 -0.16091419491957737 1103 88-89 0.03198032545344276 1103 90-91 0.1519907194971779 1103 92-93 0.03586407595438956 1103 94-95 -0.05036224257864319 1103 96-97 -0.2593930606953201 1103 98-99 -0.31523295171116317 1103 100-101 -0.7693351036261085 1104 1 0.10609669169349445 1104 2 0.05930500763605551 1104 3 0.13437496204507227 1104 4 0.05011561612904103 1104 5 3.190711533704871E-4 1104 6 0.011777578983277692 1104 7 0.08440940312850387 1104 8 0.08409756385761824 1104 9 0.09067733640508635 1104 10-11 0.1514703789193277 1104 12-13 0.12451086077776097 1104 14-15 0.018371226338778968 1104 16-17 0.07770749239791286 1104 18-19 0.16663329810256755 1104 20-21 0.15391808626618797 1104 22-23 0.12179030972485094 1104 24-25 0.12168484334952012 1104 26-27 0.15715985877550764 1104 28-29 0.16878949050573056 1104 30-31 0.24431684241743312 1104 32-33 0.1840037265814587 1104 34-35 0.21387780372419485 1104 36-37 0.17140114720083233 1104 38-39 0.22100236344616775 1104 40-41 0.13087171685445043 1104 42-43 0.16562783761667532 1104 44-45 0.1700850371448368 1104 46-47 0.13243586629923954 1104 48-49 0.2636798570778822 1104 50-51 0.16318504291110258 1104 52-53 0.20720902480331205 1104 54-55 0.17036567315619067 1104 56-57 0.17734308173270819 1104 58-59 0.11800158791258042 1104 60-61 0.1641022910660226 1104 62-63 0.3154692091227531 1104 64-65 0.28029251301231994 1104 66-67 0.24166182543245185 1104 68-69 0.1666327872842146 1104 70-71 0.25938571234737395 1104 72-73 0.007823718847298267 1104 74-75 -0.109187901688788 1104 76-77 0.009884867950344045 1104 78-79 0.34949171723954464 1104 80-81 0.37110757566481567 1104 82-83 0.36668718939295886 1104 84-85 0.09856929033466244 1104 86-87 0.21685626359940002 1104 88-89 0.3347094439464797 1104 90-91 0.36736176455950087 1104 92-93 0.11413366029819727 1104 94-95 0.2423092367513462 1104 96-97 -0.07220576161461523 1104 98-99 -0.35530999192321744 1104 100-101 -0.664953399266512 1105 1 -0.0019912288167276415 1105 2 0.011366760024301925 1105 3 0.01601994055764777 1105 4 0.022704782088588615 1105 5 0.022708910476723076 1105 6 0.04795681419528108 1105 7 0.05369919591858263 1105 8 -0.014780053638887125 1105 9 0.028522900689431196 1105 10-11 0.006202703119363662 1105 12-13 -0.04975788255018898 1105 14-15 0.024498070561811858 1105 16-17 0.08054372265549148 1105 18-19 0.16209318368546377 1105 20-21 0.09586186312546374 1105 22-23 0.007074628012425421 1105 24-25 0.015652379549671025 1105 26-27 0.03300383545413155 1105 28-29 0.01690204627723091 1105 30-31 0.05126135833454981 1105 32-33 0.1547182039033359 1105 34-35 0.20417260568407158 1105 36-37 0.13205042319788163 1105 38-39 0.08267197722002351 1105 40-41 0.18383501031515692 1105 42-43 0.23021306193441404 1105 44-45 0.24025677228841147 1105 46-47 0.28331983421865203 1105 48-49 0.3321329269051674 1105 50-51 0.19963066741497926 1105 52-53 0.11319342247116992 1105 54-55 0.017267049993570538 1105 56-57 0.08834464196592506 1105 58-59 0.09817786689323071 1105 60-61 -0.09715202694783187 1105 62-63 -0.0018090872120595236 1105 64-65 0.042389028491470526 1105 66-67 0.023362972340727595 1105 68-69 -0.19799645203366367 1105 70-71 -0.1748196362415726 1105 72-73 -0.3489238792147269 1105 74-75 -0.34564907377977505 1105 76-77 -0.5202598231508553 1105 78-79 -0.5211367009029573 1105 80-81 -0.18813015460644777 1105 82-83 -0.12503864752160965 1105 84-85 -0.262674078674177 1105 86-87 -0.35814343530296355 1105 88-89 -0.33930911461701285 1105 90-91 0.01981592928728304 1105 92-93 0.005359018193797027 1105 94-95 0.041629194050226914 1105 96-97 -0.10868850324546742 1105 98-99 -0.19009202460599894 1105 100-101 -1.1109954981907713 1106 1 0.007293669077277798 1106 2 0.008096334970439045 1106 3 -0.012638682450401006 1106 4 0.014848200038684922 1106 5 0.047017316861094116 1106 6 0.05282833864256986 1106 7 0.0925856334027273 1106 8 0.08439897102965688 1106 9 0.09063098250265256 1106 10-11 0.07732795281588523 1106 12-13 0.09380887685210126 1106 14-15 0.10418212591422815 1106 16-17 0.02360351653612014 1106 18-19 0.05725549777680783 1106 20-21 0.04782222767762789 1106 22-23 0.1622678403648834 1106 24-25 0.1253470152541496 1106 26-27 0.0708517762142975 1106 28-29 0.15990620301007397 1106 30-31 0.14338730782557008 1106 32-33 0.059383930047324895 1106 34-35 0.08165650122226253 1106 36-37 0.11813955204983273 1106 38-39 0.2244594673953273 1106 40-41 0.3539919539642753 1106 42-43 0.27795391702709793 1106 44-45 0.2177913200211279 1106 46-47 0.12503067017630798 1106 48-49 0.09514529191273624 1106 50-51 0.0899532466973838 1106 52-53 0.20533500274782313 1106 54-55 -0.08123626003651196 1106 56-57 -0.1866888533291018 1106 58-59 -0.04453986625419759 1106 60-61 -0.11281122259182297 1106 62-63 -0.2166238191007892 1106 64-65 -0.07698275333571303 1106 66-67 -0.07558908909587103 1106 68-69 -0.16809792751645602 1106 70-71 -0.14749068614666072 1106 72-73 -0.2109640304784861 1106 74-75 -0.17075346736126917 1106 76-77 -0.09550008839260826 1106 78-79 -0.05804869556652292 1106 80-81 0.18109118090900012 1106 82-83 0.1644805727472125 1106 84-85 -0.007088449111545714 1106 86-87 0.18578878876561689 1106 88-89 -0.07610041426825376 1106 90-91 0.1517594041418917 1106 92-93 0.0011244482611338924 1106 94-95 -0.12535966394556297 1106 96-97 -0.4258392560473361 1106 98-99 -0.3195412274042475 1106 100-101 -0.7600598583730758 1107 1 0.03039876690023391 1107 2 0.017497549374233756 1107 3 0.01312796563377816 1107 4 0.011154779805124804 1107 5 -0.06713856654361905 1107 6 0.09118070999695504 1107 7 0.07484035959597435 1107 8 0.11073593697874884 1107 9 -0.016732659954200813 1107 10-11 0.0530012469694654 1107 12-13 0.07081605659614354 1107 14-15 0.031943702544118935 1107 16-17 0.09868861269749374 1107 18-19 0.08448496502928293 1107 20-21 0.14324341002964047 1107 22-23 0.09061789593174296 1107 24-25 0.07569243683784777 1107 26-27 0.06654780281254347 1107 28-29 0.11108542850957548 1107 30-31 0.08402277678057146 1107 32-33 0.15279906646807717 1107 34-35 0.07938516316664135 1107 36-37 0.14544025886672074 1107 38-39 0.18800678431265538 1107 40-41 0.09859169396997203 1107 42-43 0.10270840140717752 1107 44-45 0.09882566230736956 1107 46-47 0.1317304000278554 1107 48-49 0.08478164102753993 1107 50-51 -0.028706983926156227 1107 52-53 0.12090237269950421 1107 54-55 -0.07635966100930602 1107 56-57 -0.0527224511117268 1107 58-59 -0.1425138331099376 1107 60-61 -0.08464821603336503 1107 62-63 -0.06354374141554331 1107 64-65 -0.04784406575712552 1107 66-67 -0.07960659917877422 1107 68-69 -0.05018470894503935 1107 70-71 -0.3907524267278788 1107 72-73 -0.5176815601873912 1107 74-75 -0.578126581670066 1107 76-77 -0.3739474511842076 1107 78-79 -0.17019632561685327 1107 80-81 -0.052003570119758535 1107 82-83 -0.07468067870918915 1107 84-85 -0.13005847270688875 1107 86-87 -0.24304991001062604 1107 88-89 -0.3997774754897314 1107 90-91 -0.16742514967568667 1107 92-93 -0.1052825772843704 1107 94-95 -0.13358070967815294 1107 96-97 -0.31516909442784424 1107 98-99 -0.2330027156970793 1107 100-101 -0.7624628391167221 1108 1 -0.14888640972372258 1108 2 -0.06941394089657393 1108 3 -0.05258540216182439 1108 4 -0.03399662955629168 1108 5 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-0.2858235001980134 2316 88-89 -0.5968644829215641 2316 90-91 -0.9069921607944238 2316 92-93 -0.7940045030752607 2316 94-95 -0.6748731389016207 2316 96-97 -0.6307587936382326 2316 98-99 -0.2841494952921302 2316 100-101 0.7803461692095297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 10.0 5 39.0 6 98.0 7 202.0 8 539.0 9 1017.0 10 1866.0 11 2859.0 12 4460.0 13 6366.0 14 7926.0 15 10710.0 16 14492.0 17 20823.0 18 21021.0 19 14552.0 20 13230.0 21 14012.0 22 14985.0 23 16481.0 24 18151.0 25 21238.0 26 25126.0 27 29582.0 28 36264.0 29 43996.0 30 54017.0 31 66419.0 32 83749.0 33 109870.0 34 154605.0 35 233206.0 36 379350.0 37 571427.0 38 356031.0 39 16419.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57293534974727 18.954494809500517 14.405334529050492 23.067235311701722 2 15.59396605376737 20.58353966256714 39.8310129750646 23.991481308600886 3 20.251341140019754 24.374627930889854 29.29807471147732 26.07595621761308 4 13.30764365250098 16.35675001014734 36.399394203839755 33.93621213351193 5 13.941810082396398 37.33641643327786 33.515055269175086 15.206718215150655 6 32.24900556074197 35.421740336350474 17.08173512738293 15.247518975524619 7 28.744833313038654 32.69400867259271 20.35044682117682 18.210711193191813 8 26.18499783523427 34.80609280080096 19.46898127477642 19.53992808918835 9 26.4576653001583 17.41287663541286 20.557072019049937 35.5723860453789 10-11 25.57381090094844 26.338032397072155 26.46237958490617 21.625777117073238 12-13 26.50353973021607 23.731286614982885 27.30274840686772 22.462425247933325 14-15 22.92360384258679 25.133148325532833 26.32703845948133 25.616209372399055 16-17 22.191568081894335 28.377679303994576 26.86926305121165 22.561489562899443 18-19 22.025941673093318 27.079232117005585 29.128043356198674 21.766782853702427 20-21 22.993448201214974 25.8549556223025 29.075382892938805 22.076213283543723 22-23 22.457943506379294 25.85751359743475 29.207890232847618 22.47665266333834 24-25 22.595555209794217 26.05171931940075 29.2641991991209 22.088526271684135 26-27 22.245446717541633 26.089249853968965 29.55632146826354 22.10898196022586 28-29 21.851945182358335 26.908641351017337 29.280477229572234 21.958936237052097 30-31 22.1843035880992 26.147558381025824 29.10388000432953 22.56425802654544 32-33 22.07133454241726 26.24580285871751 29.24461709958115 22.438245499284086 34-35 22.804385056134638 26.270926274393723 28.99086622830201 21.93382244116963 36-37 22.62712692328676 25.966173921244472 29.250093148381463 22.156606007087312 38-39 22.14187102829863 26.545497066229895 29.22757762929723 22.085054276174244 40-41 22.352968039522413 26.472824957456574 28.958303954461357 22.215903048559653 42-43 22.12932792204772 26.514925997934824 28.54018336241096 22.815562717606497 44-45 22.39619493475424 26.36030490584163 28.581370943122746 22.662129216281375 46-47 22.79445435506656 26.88030622906208 28.11872760860658 22.206511807264782 48-49 22.693294405785856 26.799967442927347 28.11476579520121 22.39197235608558 50-51 22.206345763480087 27.095470579505545 28.271596951889045 22.426586705125327 52-53 22.761370093761414 27.25334354832163 27.578460073602034 22.406826284314917 54-55 22.22730293866948 26.835843954215203 27.888271028669614 23.048582078445698 56-57 22.536836987083273 27.043866139568312 27.732596241253198 22.686700632095217 58-59 22.897717170867278 26.653350885697453 27.8168257041364 22.632106239298874 60-61 22.52427340456816 26.87719719579204 28.364076016605722 22.23445338303408 62-63 22.17120300770954 26.94844071468618 28.333739232056526 22.546617045547755 64-65 21.923561129215877 27.24791947883645 28.03708523028169 22.791434161665983 66-67 22.190999596633368 27.050573888395135 27.588776598308566 23.169649916662934 68-69 22.077844102496886 26.888825700118073 27.987683122643414 23.045647074741627 70-71 22.45150305541004 26.90016569780593 27.694576536192855 22.953754710591173 72-73 22.356914058800452 26.715238597773 28.009087788015314 22.91875955541124 74-75 21.94994232928793 27.56700203623277 27.430858566383893 23.052197068095413 76-77 22.123886127535926 26.933881084942623 27.56504109512515 23.377191692396305 78-79 22.254510886640993 27.054769177162008 27.637783488435918 23.05293644776108 80-81 22.36929809967133 27.167421342510504 27.49712649570734 22.966154062110817 82-83 22.327317652311564 26.93169403065849 27.697099383041767 23.043888933988175 84-85 22.07443749915439 27.14442877244253 27.50476502144471 23.27636870695837 86-87 22.088793080825567 27.132846354473088 27.46121424163441 23.317146323066932 88-89 21.943461862296402 27.084726533624266 27.18157651042018 23.790235093659152 90-91 22.079915792298426 26.840886422781935 27.32916116272187 23.750036622197765 92-93 22.308481653610425 26.805592198725492 27.434790660388845 23.451135487275238 94-95 22.038266462366902 26.666848473163668 27.530196790734802 23.764688273734627 96-97 21.722303682807702 26.576579433372572 27.86114803615159 23.839968847668143 98-99 21.811554511128385 26.830865162418128 27.156236265379867 24.20134406107362 100-101 22.049803743409228 25.60838274717792 27.942511733431818 24.399301775981034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 644.0 1 475.0 2 404.0 3 529.5 4 472.5 5 464.5 6 795.5 7 1645.0 8 2725.5 9 3246.5 10 2983.0 11 2671.0 12 2734.0 13 2971.5 14 3226.5 15 3391.5 16 3558.5 17 3751.0 18 4008.0 19 4317.5 20 4718.0 21 5423.5 22 6441.0 23 7770.0 24 9319.0 25 11259.5 26 13815.0 27 16857.0 28 19475.5 29 22111.0 30 25555.0 31 29648.0 32 34639.0 33 38535.5 34 42027.0 35 45945.0 36 50099.0 37 54591.5 38 58458.0 39 60872.5 40 62861.5 41 66695.5 42 70812.0 43 74285.5 44 76916.0 45 79856.5 46 82056.0 47 89917.5 48 107335.5 49 107413.5 50 93758.0 51 87831.0 52 84783.5 53 83463.5 54 81776.5 55 76328.5 56 68498.0 57 61345.5 58 55748.5 59 49878.0 60 42724.5 61 35710.5 62 29267.5 63 22729.5 64 17278.0 65 13324.5 66 9823.0 67 7856.0 68 6159.0 69 4254.5 70 3126.5 71 2321.0 72 1817.5 73 1473.5 74 1140.0 75 897.0 76 614.5 77 331.0 78 190.0 79 123.0 80 72.5 81 39.0 82 24.0 83 13.5 84 10.5 85 7.5 86 3.5 87 3.0 88 3.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42618825343994804 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011204353885078 16-17 0.002346572228761619 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.228058069840755E-5 26-27 0.0030653421006345467 28-29 4.228058069840755E-5 30-31 0.0 32-33 2.1140290349203774E-5 34-35 0.012768735370919078 36-37 0.027270974550472865 38-39 0.022514409221902017 40-41 0.009111465140506826 42-43 0.009217166592252845 44-45 0.00397437458565031 46-47 0.005285072587300943 48-49 0.0031499032620313622 50-51 6.342087104761132E-4 52-53 0.0 54-55 0.0 56-57 8.45611613968151E-5 58-59 0.0016278023568886905 60-61 0.002684816874348879 62-63 0.0012684174209522264 64-65 0.0025156945515552487 66-67 0.0024522736805076377 68-69 0.004418320682983589 70-71 1.2684174209522263E-4 72-73 0.0 74-75 0.0 76-77 0.09642086428271841 78-79 0.015770656600506014 80-81 3.593849359364641E-4 82-83 0.0 84-85 0.0 86-87 0.10815372542652649 88-89 0.42124142549823435 90-91 0.4237782603401389 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.7482377453964905E-4 100-101 3.593849359364641E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2365152.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.01856078086856 #Duplication Level Percentage of deduplicated Percentage of total 1 76.41726284267004 42.807850832597204 2 13.725182550126394 15.377299458255441 3 4.358175266200128 7.324161181299299 4 1.8592785351194026 4.166164305126021 5 0.9862287169697703 2.762355666270455 6 0.5930290504132372 1.993238034323682 7 0.395418531475354 1.5505543927533738 8 0.2750981205379546 1.2328480628846494 9 0.20364960875200377 1.0267342187286805 >10 1.0640771639101223 10.82072808913284 >50 0.07952404373856935 3.065945786211708 >100 0.038999667457266594 3.995267957767406 >500 0.002566309063239264 1.0448037745202627 >1k 0.001283154531619475 1.670206147200373 >5k 1.509593566611147E-4 0.7259927482198022 >10k+ 7.547967833055735E-5 0.4358493447087781 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10308 0.43582822583918496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9523 0.40263796999093504 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7647 0.3233196006007225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4878 0.206244672646832 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4717 0.1994374991543884 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3384 0.14307748508341114 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3203 0.13542469997699938 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2821 0.11927351815020769 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 4.228058069840755E-5 9 0.0 0.0 0.0 0.0 4.228058069840755E-5 10-11 0.0 0.0 0.0 4.228058069840755E-5 4.228058069840755E-5 12-13 0.0 0.0 0.0 4.228058069840755E-5 1.479820324444264E-4 14-15 0.0 0.0 0.0 4.228058069840755E-5 2.325431938412415E-4 16-17 6.342087104761132E-5 0.0 0.0 8.45611613968151E-5 2.959640648888528E-4 18-19 8.45611613968151E-5 0.0 0.0 1.2684174209522263E-4 3.382446455872604E-4 20-21 8.45611613968151E-5 0.0 0.0 1.2684174209522263E-4 3.382446455872604E-4 22-23 8.45611613968151E-5 0.0 0.0 2.1140290349203772E-4 4.2280580698407543E-4 24-25 8.45611613968151E-5 0.0 0.0 4.8622667803168676E-4 5.073669683808905E-4 26-27 8.45611613968151E-5 0.0 0.0 0.0011204353885077999 5.073669683808905E-4 28-29 8.45611613968151E-5 0.0 0.0 0.003319025584824992 5.073669683808905E-4 30-31 8.45611613968151E-5 0.0 0.0 0.01109865243333198 5.285072587300943E-4 32-33 1.0570145174601886E-4 0.0 0.0 0.024459315934028763 5.496475490792981E-4 34-35 2.959640648888528E-4 0.0 0.0 0.04445803060437553 5.919281297777056E-4 36-37 2.959640648888528E-4 0.0 0.0 0.08043880477872035 5.919281297777056E-4 38-39 2.959640648888528E-4 0.0 0.0 0.13481163155687245 6.130684201269094E-4 40-41 2.959640648888528E-4 0.0 0.0 0.19260918537159558 6.342087104761132E-4 42-43 3.805252262856679E-4 0.0 0.0 0.2520134012528582 6.342087104761132E-4 44-45 4.2280580698407543E-4 0.0 0.0 0.3257718742812301 6.55349000825317E-4 46-47 4.439460973332792E-4 0.0 0.0 0.4174573135257269 7.187698718729282E-4 48-49 4.65086387682483E-4 0.0 0.0 0.5133073899690168 7.610504525713358E-4 50-51 4.65086387682483E-4 0.0 0.0 0.6237442667532573 7.610504525713358E-4 52-53 4.65086387682483E-4 0.0 0.0 0.7319614130508314 7.821907429205395E-4 54-55 4.65086387682483E-4 0.0 0.0 0.853919748075388 8.033310332697433E-4 56-57 4.65086387682483E-4 0.0 0.0 1.0021131834233064 8.033310332697433E-4 58-59 4.8622667803168676E-4 0.0 0.0 1.1641535089499533 8.033310332697433E-4 60-61 5.073669683808905E-4 0.0 0.0 1.3418165090446617 8.033310332697433E-4 62-63 5.073669683808905E-4 0.0 0.0 1.5235595851767667 8.033310332697433E-4 64-65 5.073669683808905E-4 0.0 0.0 1.7217075266198536 8.033310332697433E-4 66-67 5.073669683808905E-4 0.0 0.0 1.945836884902112 8.033310332697433E-4 68-69 6.342087104761132E-4 0.0 0.0 2.207469118263858 8.033310332697433E-4 70-71 6.342087104761132E-4 0.0 0.0 2.4982115314364575 8.033310332697433E-4 72-73 6.342087104761132E-4 0.0 0.0 2.792420952226326 8.456116139681509E-4 74-75 6.342087104761132E-4 0.0 0.0 3.1025278713587965 8.878921946665584E-4 76-77 6.342087104761132E-4 0.0 0.0 3.436206214230629 8.878921946665584E-4 78-79 6.342087104761132E-4 0.0 0.0 3.8263502726251843 9.30172775364966E-4 80-81 6.764892911745208E-4 0.0 0.0 4.251756335322211 0.001035874227110985 82-83 6.764892911745208E-4 0.0 0.0 4.685660794739619 0.0010992950981585962 84-85 6.764892911745208E-4 0.0 0.0 5.123307085548836 0.0010992950981585962 86-87 6.764892911745208E-4 0.0 0.0 5.57693543586205 0.0011204353885078 88-89 6.764892911745208E-4 0.0 0.0 6.0823363572404645 0.0011415756788570037 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10805 0.0 23.993748 1 TACATGG 11025 0.0 22.899017 2 ACATGGG 11145 0.0 21.928606 3 GAGTACT 9570 0.0 20.854614 12-13 AGTACTT 10015 0.0 20.354494 12-13 CATGGGG 5205 0.0 20.331432 4 GTATCAA 21285 0.0 19.926926 1 GTACTTT 10195 0.0 19.576132 14-15 AGAGTAC 15765 0.0 19.493479 10-11 GTACGCT 125 0.0060659535 18.982082 3 ATGGGGA 2860 0.0 18.417927 5 GAGTACA 9290 0.0 18.262417 1 AGTACAT 8685 0.0 17.976698 2 GTAAGCG 400 0.0 17.796831 94-95 TACTTTT 11415 0.0 17.795736 14-15 TGCACCG 650 0.0 17.521921 5 CAACGCA 24150 0.0 17.488667 5 TCAACGC 24235 0.0 17.42733 4 ATCAACG 24250 0.0 17.357841 3 TATCAAC 24360 0.0 17.357384 2 >>END_MODULE