FastQCFastQC Report
Fri 27 May 2016
SRR939177_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939177_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122544
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153590.7236127967194084No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132820.6257585237337836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110750.5217795249474215No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT87370.4116286870849321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76200.3590031584739822No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59330.27952306289056905No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47350.223081358973006No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40590.19123278480917238No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37850.1783237473522339No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35660.1680059400417612No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34930.1645666709382703No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33650.15853617168831363No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30840.1452973413036432No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29780.14030333411227283No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28370.13366036228224246No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28060.13219985074514357No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.11561597780776277No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24290.1144381459230056No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22090.1040732253371426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21230.10002148365357798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT106500.023.1681512-13
GTACATG151350.022.2378231
AGTACTT111050.021.98433512-13
TACATGG149950.021.927562
GTACTTT114950.021.36507814-15
ACATGGG152050.020.721093
GTATCGC1150.003788442620.5913896
GTGTAGC10650.020.0581761
CATGGGA100000.018.9512274
ACTTTTT133450.018.83140416-17
ATGGGAT37600.018.6488135
TACTTTT131250.018.53129814-15
GTATAGG7200.018.4609491
TAGCGCG1559.795808E-418.3398974
GTAAGCG1608.163624E-917.83450994-95
AGAGTAC238850.017.53700310-11
CATGGGG49150.017.3510744
TACCTGG14400.017.1087472
GTATCAA340000.016.8801331
TATCGCT2002.8245035E-416.5760677
Produced by FastQC (version 0.11.5)