##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939177_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122544 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.215858893855675 33.0 31.0 34.0 31.0 34.0 2 32.29972947557271 34.0 31.0 34.0 31.0 34.0 3 32.34322586481128 34.0 31.0 34.0 30.0 34.0 4 35.78459857604837 37.0 35.0 37.0 35.0 37.0 5 35.84491346233576 37.0 35.0 37.0 35.0 37.0 6 35.944372884613934 37.0 37.0 37.0 35.0 37.0 7 35.88448720026534 37.0 37.0 37.0 35.0 37.0 8 35.87951910537544 37.0 37.0 37.0 35.0 37.0 9 37.57138226580933 39.0 38.0 39.0 35.0 39.0 10-11 37.49356032195328 39.0 38.0 39.0 35.0 39.0 12-13 37.41900733271018 39.0 38.0 39.0 35.0 39.0 14-15 38.70342075358626 40.0 38.0 41.0 35.0 41.0 16-17 38.66372734793719 40.0 38.0 41.0 35.0 41.0 18-19 38.666346846048896 40.0 38.5 41.0 35.0 41.0 20-21 38.59433326234934 40.0 38.0 41.0 34.0 41.0 22-23 38.509215827799096 40.0 38.0 41.0 34.0 41.0 24-25 38.529267002238825 40.0 38.0 41.0 34.0 41.0 26-27 38.42910889008661 40.0 38.0 41.0 34.0 41.0 28-29 38.313437789746644 40.0 38.0 41.0 34.0 41.0 30-31 38.13246415622008 40.0 38.0 41.0 34.0 41.0 32-33 37.89008260841707 40.0 37.5 41.0 33.5 41.0 34-35 37.56899856964096 40.0 37.0 41.0 33.0 41.0 36-37 37.30054076617493 40.0 37.0 41.0 32.5 41.0 38-39 37.058260511914 40.0 36.5 41.0 31.5 41.0 40-41 36.911111618887524 40.0 36.0 41.0 31.0 41.0 42-43 36.678348952954565 40.0 36.0 41.0 30.5 41.0 44-45 36.342449673599226 40.0 36.0 41.0 30.0 41.0 46-47 35.87146556208022 39.0 35.0 41.0 28.5 41.0 48-49 35.7269161440234 39.5 35.0 41.0 27.0 41.0 50-51 34.682970294137604 38.5 34.0 40.0 25.5 40.5 52-53 34.834613322503564 38.5 35.0 40.0 25.5 40.5 54-55 35.33914844639263 39.0 35.0 41.0 26.0 41.0 56-57 35.21846190232098 39.0 35.0 41.0 26.0 41.0 58-59 35.039026517235925 39.0 35.0 41.0 26.0 41.0 60-61 34.73281001477473 38.5 35.0 41.0 25.0 41.0 62-63 34.395702044339245 38.0 34.5 40.0 23.5 41.0 64-65 34.03060761049005 37.5 34.0 40.0 22.5 41.0 66-67 33.61921613874671 37.0 34.0 40.0 21.5 41.0 68-69 33.21384527246549 36.0 34.0 39.0 20.5 41.0 70-71 32.78875490920329 36.0 33.0 39.0 18.5 41.0 72-73 32.34472218243768 35.0 33.0 38.5 17.0 40.0 74-75 31.91367905682992 35.0 32.5 37.0 12.5 39.5 76-77 31.496524218108082 35.0 32.0 37.0 9.0 39.0 78-79 31.079553592293024 35.0 32.0 36.0 6.5 39.0 80-81 30.663278358422723 35.0 31.0 36.0 2.0 37.5 82-83 30.255831916794186 35.0 31.0 35.5 2.0 37.0 84-85 29.888668739022606 34.5 31.0 35.0 2.0 37.0 86-87 29.55123215349128 34.0 30.5 35.0 2.0 36.0 88-89 29.162909697042792 34.0 30.0 35.0 2.0 36.0 90-91 28.8129588833023 34.0 29.5 35.0 2.0 36.0 92-93 28.440073327101818 34.0 29.0 35.0 2.0 35.0 94-95 28.028872428557428 34.0 29.0 35.0 2.0 35.0 96-97 27.515496969674125 34.0 28.0 35.0 2.0 35.0 98-99 26.907144445533284 34.0 26.5 35.0 2.0 35.0 100-101 24.530242247039403 31.5 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24385614552467416 1101 2 0.3491548301111038 1101 3 0.32293776896622717 1101 4 0.19520425352545345 1101 5 0.31098358176824803 1101 6 0.3686813975606711 1101 7 0.21042707146614958 1101 8 0.36180716158257553 1101 9 0.4036815882926561 1101 10-11 0.4158566397700554 1101 12-13 0.3912129618952278 1101 14-15 0.41297434223088914 1101 16-17 0.5487729899823464 1101 18-19 0.5082662464403356 1101 20-21 0.5327627075354187 1101 22-23 0.5288041339546012 1101 24-25 0.5180205032708827 1101 26-27 0.46442676583372844 1101 28-29 0.5246565455283729 1101 30-31 0.5421182353855372 1101 32-33 0.5188043381715559 1101 34-35 0.5640525047233282 1101 36-37 0.4317934732303641 1101 38-39 0.5255206317773045 1101 40-41 0.3689978393021178 1101 42-43 0.37311094232718744 1101 44-45 0.2977688363891673 1101 46-47 0.4206452862656107 1101 48-49 0.5571422452539352 1101 50-51 0.45730438793806627 1101 52-53 0.40756435798868296 1101 54-55 0.3438434403820736 1101 56-57 0.2629486515814108 1101 58-59 0.17018729135141797 1101 60-61 0.22569180740323702 1101 62-63 0.3601321005772675 1101 64-65 0.3050175470718912 1101 66-67 0.2616031993894339 1101 68-69 0.24834534529848185 1101 70-71 0.2776903360011289 1101 72-73 0.08820229012449943 1101 74-75 0.04432773885964636 1101 76-77 0.08459327110019288 1101 78-79 0.12050401395245203 1101 80-81 -0.12675327733784414 1101 82-83 -0.18686787309332686 1101 84-85 -0.06147057264886513 1101 86-87 -0.27581307611089656 1101 88-89 -0.3296503287006658 1101 90-91 -0.4023822508967072 1101 92-93 -0.4968933835019911 1101 94-95 -0.52046966385468 1101 96-97 -0.6560404097330483 1101 98-99 -0.6688788488039208 1101 100-101 -0.8059264119765395 1102 1 0.1729045407224561 1102 2 0.2179798647330884 1102 3 0.2516966317071123 1102 4 0.29928172387732843 1102 5 0.18992427089206387 1102 6 0.15523593694196336 1102 7 0.2189847365788964 1102 8 0.23403770743964003 1102 9 0.24508424199476764 1102 10-11 0.2107730288919285 1102 12-13 0.15544637747610324 1102 14-15 0.3006183770089237 1102 16-17 0.40146100931215045 1102 18-19 0.4032996066747856 1102 20-21 0.5257954899240431 1102 22-23 0.41758359312015614 1102 24-25 0.37019010711733813 1102 26-27 0.42353838272925515 1102 28-29 0.4982545379407881 1102 30-31 0.4834735886273265 1102 32-33 0.46631606575991924 1102 34-35 0.3901771402172045 1102 36-37 0.40177291947363614 1102 38-39 0.3818508170293171 1102 40-41 0.32301886606344965 1102 42-43 0.4854792507749508 1102 44-45 0.48117576176171895 1102 46-47 0.5452304929247376 1102 48-49 0.5283544902215525 1102 50-51 0.5089644451374866 1102 52-53 0.5747567038476973 1102 54-55 0.4533709782712094 1102 56-57 0.3762030817327755 1102 58-59 0.3301617454455865 1102 60-61 0.3513775421695655 1102 62-63 0.34184096380578666 1102 64-65 0.34393390937390933 1102 66-67 0.3865012534875092 1102 68-69 0.24828027668843333 1102 70-71 0.14302071386840964 1102 72-73 0.2917687509285116 1102 74-75 0.31095010198088957 1102 76-77 0.0829999282742584 1102 78-79 0.21606907768616423 1102 80-81 0.26647355275109774 1102 82-83 0.08173519680407537 1102 84-85 0.09673880015678549 1102 86-87 -0.022388211916855028 1102 88-89 -0.07337174118018552 1102 90-91 0.0056765650313579386 1102 92-93 0.12951770626160553 1102 94-95 -0.13522051965167137 1102 96-97 -0.3693322657641005 1102 98-99 -0.6214468797881629 1102 100-101 -0.505205201864964 1103 1 0.24519128428534742 1103 2 0.2901157333536588 1103 3 0.3153605635081149 1103 4 0.26948701085294147 1103 5 0.242001712970648 1103 6 0.21124230368806707 1103 7 0.27062190616497617 1103 8 0.28072129680451496 1103 9 0.3403251522206503 1103 10-11 0.3599348200627688 1103 12-13 0.27679129965198257 1103 14-15 0.37175752987994315 1103 16-17 0.4293794909631927 1103 18-19 0.5111437213903329 1103 20-21 0.468719469834447 1103 22-23 0.5329821897185028 1103 24-25 0.5692882690605572 1103 26-27 0.43501855414437074 1103 28-29 0.384967014122104 1103 30-31 0.39449810595250057 1103 32-33 0.43263845521902056 1103 34-35 0.37794068241969114 1103 36-37 0.4215012151720501 1103 38-39 0.7123278200706409 1103 40-41 0.5820767299860208 1103 42-43 0.6852150284588063 1103 44-45 0.45179691066410754 1103 46-47 0.42101821158679087 1103 48-49 0.39181252037125347 1103 50-51 0.367454778112311 1103 52-53 0.46781313313484674 1103 54-55 0.4654956768521856 1103 56-57 0.34935043008360367 1103 58-59 0.2803230169360731 1103 60-61 0.3391638773701615 1103 62-63 0.2270401319642943 1103 64-65 0.10375732392972026 1103 66-67 0.16591870563030398 1103 68-69 0.11729244579660048 1103 70-71 0.20958759131885074 1103 72-73 -0.00798314361078667 1103 74-75 -0.056026284767170864 1103 76-77 -0.0182764207991859 1103 78-79 0.13035828759850077 1103 80-81 0.15892104135974705 1103 82-83 0.16074675711861275 1103 84-85 0.2547036883519809 1103 86-87 0.17735594770824648 1103 88-89 0.32323613131548967 1103 90-91 0.20707358464833447 1103 92-93 0.172338985334072 1103 94-95 0.11190440097722743 1103 96-97 7.201463674242348E-4 1103 98-99 -0.03672310004440149 1103 100-101 0.016475792039155834 1104 1 0.21583800342740744 1104 2 0.17251871688921483 1104 3 0.19864778036440356 1104 4 0.23471318209688263 1104 5 0.196607631920223 1104 6 0.24904091503787384 1104 7 0.1738460494296774 1104 8 0.2541252656859214 1104 9 0.2883177964993422 1104 10-11 0.3042198141621526 1104 12-13 0.33256459563383345 1104 14-15 0.4066364812886434 1104 16-17 0.3831076214413187 1104 18-19 0.31731473618197725 1104 20-21 0.3255189290399798 1104 22-23 0.2824764838786038 1104 24-25 0.21713239536814655 1104 26-27 0.2899278106665477 1104 28-29 0.4210067544949325 1104 30-31 0.3871837748992135 1104 32-33 0.30864571355149195 1104 34-35 0.31359301574156717 1104 36-37 0.3929684352364404 1104 38-39 0.49611214963445605 1104 40-41 0.3913382648340331 1104 42-43 0.5075037519776444 1104 44-45 0.3160106676961547 1104 46-47 0.21442140708627733 1104 48-49 0.3779890347676158 1104 50-51 0.2994740782876093 1104 52-53 0.4312191357242412 1104 54-55 0.45816358665867085 1104 56-57 0.39889327544159414 1104 58-59 0.46692575069791786 1104 60-61 0.5644123621144814 1104 62-63 0.5152232859875951 1104 64-65 0.4506874273910384 1104 66-67 0.29734479323442287 1104 68-69 0.35545139583039287 1104 70-71 0.4069040992989983 1104 72-73 0.42272570387829944 1104 74-75 0.3983420340724102 1104 76-77 0.42882769359259143 1104 78-79 0.34245856372448813 1104 80-81 0.39990183284452385 1104 82-83 0.3032572693838773 1104 84-85 0.3675591575942967 1104 86-87 0.18395017426904658 1104 88-89 0.30603783619294944 1104 90-91 0.17241590639812898 1104 92-93 0.13604259674117003 1104 94-95 -0.04320665093674947 1104 96-97 0.013281873701604496 1104 98-99 0.05091954595291526 1104 100-101 0.08075671872255086 1105 1 0.17149979692695183 1105 2 0.16096670018121984 1105 3 0.2579100611806524 1105 4 0.2347643068412637 1105 5 0.22764796608025506 1105 6 0.2547222882267519 1105 7 0.2353697843641953 1105 8 0.21619179311924341 1105 9 0.26889256915739423 1105 10-11 0.36063107465904665 1105 12-13 0.3932784928622226 1105 14-15 0.4172845690056519 1105 16-17 0.3489220277416294 1105 18-19 0.3802210147356888 1105 20-21 0.5098530020504697 1105 22-23 0.40803504890492803 1105 24-25 0.3470789863266788 1105 26-27 0.397235385830804 1105 28-29 0.4051216437609071 1105 30-31 0.2695571595748376 1105 32-33 0.28570973913562625 1105 34-35 0.23901724234384147 1105 36-37 0.23296158234516895 1105 38-39 0.26188413327453475 1105 40-41 0.14289865729802642 1105 42-43 0.0036416582561926703 1105 44-45 -0.100851251983606 1105 46-47 -0.06799657577061424 1105 48-49 -0.02212735560788559 1105 50-51 -0.1028499220604715 1105 52-53 -0.2218902277183048 1105 54-55 -0.16180824034390184 1105 56-57 -0.06202784016559093 1105 58-59 -0.18733500725361552 1105 60-61 -0.16640987606155733 1105 62-63 -0.06462323100321754 1105 64-65 -0.06890803447207361 1105 66-67 -0.09861798360062579 1105 68-69 -0.12660273113301201 1105 70-71 -0.25352371691298003 1105 72-73 -0.255981927849497 1105 74-75 -0.1978078375718475 1105 76-77 -0.2694318895775787 1105 78-79 -0.3630487677814074 1105 80-81 -0.5954724655499426 1105 82-83 -0.40174642900188573 1105 84-85 -0.37609430365967356 1105 86-87 -0.4450696447276066 1105 88-89 -0.5370809907661069 1105 90-91 -0.5139517349019656 1105 92-93 -0.5243797806682196 1105 94-95 -0.4677894947778505 1105 96-97 -0.5274765129319938 1105 98-99 -0.5331877289894322 1105 100-101 -0.64965815313926 1106 1 0.22324471785059075 1106 2 0.25522480049875185 1106 3 0.322813823345264 1106 4 0.2373513730516592 1106 5 0.2156015376189302 1106 6 0.2344707236554342 1106 7 0.22148851708711703 1106 8 0.19522705120927952 1106 9 0.26097362885259656 1106 10-11 0.25437484463365934 1106 12-13 0.2585709572290469 1106 14-15 0.36947728103204014 1106 16-17 0.3634013774554319 1106 18-19 0.327814041217799 1106 20-21 0.34066914822098937 1106 22-23 0.4011830486171917 1106 24-25 0.46669804275401106 1106 26-27 0.43898426314169825 1106 28-29 0.3418356329296586 1106 30-31 0.38382789977892884 1106 32-33 0.37466062855202864 1106 34-35 0.34102806331988944 1106 36-37 0.28927844272066494 1106 38-39 0.32620008933294287 1106 40-41 0.3175531156840279 1106 42-43 0.2349107336947398 1106 44-45 0.17014971399147072 1106 46-47 0.3968000556482352 1106 48-49 0.47921858678300566 1106 50-51 0.3567404634046838 1106 52-53 0.30872501619040804 1106 54-55 0.30317721864840763 1106 56-57 0.3635997867214016 1106 58-59 0.4987444185050407 1106 60-61 0.5466279613874434 1106 62-63 0.5747857258464677 1106 64-65 0.41088869971007824 1106 66-67 0.36069211494938 1106 68-69 0.3832365984391899 1106 70-71 0.33941104894083196 1106 72-73 0.26095808920920405 1106 74-75 0.24456918044256426 1106 76-77 0.32861920433780867 1106 78-79 0.2854501172367314 1106 80-81 0.4133854744568417 1106 82-83 0.45954134935821855 1106 84-85 0.34552478359665173 1106 86-87 0.2886585566062614 1106 88-89 0.5003551675304898 1106 90-91 0.4838093095842737 1106 92-93 0.3576011875676457 1106 94-95 0.16568488119198221 1106 96-97 0.4352828359597005 1106 98-99 0.5042353269857394 1106 100-101 0.16237491609237864 1107 1 0.12421317864028225 1107 2 0.08540915392764958 1107 3 0.14344944985539598 1107 4 0.013547990356471473 1107 5 0.049762509637325536 1107 6 0.03311537266251463 1107 7 0.03251464082280364 1107 8 0.12350067102912732 1107 9 0.20632131121722352 1107 10-11 0.1417477959190947 1107 12-13 0.16046711451497941 1107 14-15 0.15804969077001374 1107 16-17 0.1803536412325215 1107 18-19 0.27628772724873585 1107 20-21 0.24400885560748264 1107 22-23 0.2284039614408968 1107 24-25 0.2084253617481977 1107 26-27 0.16125095550221857 1107 28-29 0.2222994070608877 1107 30-31 0.2073659439822464 1107 32-33 0.1249276964381707 1107 34-35 0.18081722545306178 1107 36-37 0.2580715231671462 1107 38-39 0.3808343718566718 1107 40-41 0.1821123919078218 1107 42-43 0.13358824119290347 1107 44-45 0.25176847643692923 1107 46-47 0.27017701912836856 1107 48-49 0.24840473384081463 1107 50-51 0.18223914659447615 1107 52-53 0.3272030998239117 1107 54-55 0.5311763588923952 1107 56-57 0.3389485067225806 1107 58-59 0.28155053364316984 1107 60-61 0.29885319769682184 1107 62-63 0.39833093468318026 1107 64-65 0.25025514847307306 1107 66-67 0.07836034795314362 1107 68-69 0.35201460844991317 1107 70-71 0.22768303818893543 1107 72-73 0.22966544111130105 1107 74-75 0.27183703396780956 1107 76-77 0.1830160657342681 1107 78-79 0.26461906917439393 1107 80-81 0.18429978050033569 1107 82-83 0.2166827594568943 1107 84-85 0.11353370727106693 1107 86-87 0.15829508734254105 1107 88-89 0.1326199822629519 1107 90-91 0.055622406534112656 1107 92-93 0.20155991927929762 1107 94-95 0.04974900713141395 1107 96-97 0.17699607274342455 1107 98-99 -0.02182491522827945 1107 100-101 0.06496925454965918 1108 1 0.09050205717880999 1108 2 0.1902780572129501 1108 3 0.20856073974544387 1108 4 0.08866055051793609 1108 5 0.15122943788581011 1108 6 0.17827587380811138 1108 7 0.29937796432329833 1108 8 0.23884225642194679 1108 9 0.2553594334737852 1108 10-11 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1412.0 4 1287.0 5 1499.0 6 1777.0 7 2051.0 8 2609.0 9 3167.0 10 3980.0 11 5281.0 12 7803.0 13 12723.0 14 11368.0 15 12722.0 16 17022.0 17 25323.0 18 23806.0 19 12825.0 20 11525.0 21 11865.0 22 12356.0 23 13242.0 24 14674.0 25 16456.0 26 18867.0 27 22163.0 28 25998.0 29 30872.0 30 37294.0 31 46036.0 32 57998.0 33 77181.0 34 108737.0 35 166454.0 36 272430.0 37 453931.0 38 475635.0 39 95437.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24605819603082 19.436116634096027 15.329237657901428 20.98858751197172 2 14.83720787628506 21.4743624515633 40.3503715740542 23.338058098097434 3 19.577202717713007 26.512181834904357 30.396171996047478 23.514443451335158 4 12.289682992659483 17.463328072361787 38.605263927502364 31.641725007476367 5 12.879332916602781 38.69178057956998 33.594301013127016 14.834585490700224 6 29.288137671198967 37.20866409991017 17.961526945048934 15.541671283841929 7 26.826549210922817 34.648186879130776 21.119727990606055 17.40553591934035 8 25.90465285071347 33.32042695737925 20.23090937130229 20.544010820604992 9 26.859026925374852 17.558198260052748 21.395909440503548 34.18686537406885 10-11 24.63314131100332 27.05100525847095 26.87665027171792 21.439203158807803 12-13 26.779743101130293 25.016540122352104 27.34745951737267 20.856257259144932 14-15 23.427580050448725 25.750234960522388 27.656657246239096 23.165527742789788 16-17 20.825901966548887 29.218621400025885 27.95725568200438 21.998220951420848 18-19 20.789272079436525 27.878096784910817 30.78476603589131 20.54786509976135 20-21 22.681616494331912 26.10289054526452 30.229232577399717 20.98626038300385 22-23 21.819147879935034 26.2682738909848 30.42911644090672 21.483461788173443 24-25 21.984007102503238 26.47755748612144 30.551260109367984 20.987175302007344 26-27 21.62850508952065 26.19897860504961 31.27453598044386 20.89798032498587 28-29 20.891762108719032 27.303392516839942 30.896691447008433 20.908153927432593 30-31 21.31231357574225 26.573675432655207 30.5898741795446 21.524136812057932 32-33 21.29464013052848 26.68889147237648 30.600336225450743 21.416132171644296 34-35 22.05923573050862 26.521247958631417 30.256072111419137 21.163444199440825 36-37 21.95089445921033 26.129093193626062 30.826152125766843 21.093860221396763 38-39 21.53004572175049 26.72956957184373 30.606049849014095 21.134334857391682 40-41 21.429088580891445 26.925295555930973 30.492456775699278 21.15315908747831 42-43 21.125119790659536 27.069813369170138 29.613079424077043 22.191987416093284 44-45 21.590808493716818 26.70119320105 29.67465684522471 22.033341460008472 46-47 22.205362796560518 27.193982684238872 29.154320951124102 21.446333568076508 48-49 21.955439227627167 27.099761983336467 29.072407348924283 21.872391440112082 50-51 21.474375513964446 27.53450456076131 29.41502856896675 21.576091356307494 52-53 21.831861788229123 28.04078158148528 28.339335823336942 21.788020806948655 54-55 21.3496262517743 27.383776621617113 28.76243549260184 22.504161634006746 56-57 22.027963836561703 27.53494887968356 28.22442534753651 22.21266193621822 58-59 22.494254336633176 27.104389133184405 28.372788386875143 22.02856814330728 60-61 21.917492019239912 27.287104444487543 29.30032774447567 21.49507579179688 62-63 21.587829762459794 27.438129971434588 29.179578689777745 21.794461576327866 64-65 21.37078102672928 27.84936644972269 28.826703332187538 21.953149191360495 66-67 21.623626999335666 27.44753630517513 28.258019242488597 22.67081745300061 68-69 21.392099216324397 27.485167561096198 28.693831372407875 22.428901850171528 70-71 21.989019187417973 27.48742822683009 28.33313878920311 22.190413796548828 72-73 21.762571533281502 27.02267663026752 28.860264001962165 22.354487834488822 74-75 21.309456697948832 28.39511989656215 28.1415446873626 22.153878718126414 76-77 21.62346860226314 27.343124936679974 28.299505843432186 22.7339006176247 78-79 21.604878814176846 27.48010686664838 28.573054770695595 22.34195954847918 80-81 21.90549669791059 27.669225426029293 28.12144778367735 22.30383009238277 82-83 21.70122599538157 27.42039172393943 28.53885569346044 22.339526587218554 84-85 21.555460246361644 27.444645319616384 28.34280823490371 22.657086199118265 86-87 21.51341253730439 27.69670963930254 28.276040167547833 22.513837655845236 88-89 21.576359764290796 27.39669730608581 27.922620868199992 23.104322061423403 90-91 21.574090757451742 26.992480010266263 28.43307147577283 23.000357756509167 92-93 21.916247281205557 27.275708188688064 28.401863802100415 22.406180728005967 94-95 21.62379215672533 27.045153426756613 28.50694802876379 22.82410638775427 96-97 21.29452167499873 26.923190751116632 28.83301035006493 22.94927722381971 98-99 21.537340443363608 27.19385344132852 28.024177701513203 23.244628413794672 100-101 21.679843955648558 26.280453740614142 28.745821931427596 23.2938803723097 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3403.0 1 2044.0 2 803.5 3 896.5 4 769.0 5 805.5 6 1419.5 7 3061.0 8 4957.5 9 5568.0 10 4902.0 11 4301.5 12 4400.5 13 4673.0 14 4757.0 15 4807.5 16 5001.5 17 5147.0 18 5333.0 19 5618.0 20 5952.0 21 6491.5 22 7411.0 23 8619.0 24 9818.5 25 11506.0 26 14369.0 27 17620.5 28 19945.0 29 22625.5 30 26506.5 31 30583.0 32 35079.0 33 39486.0 34 44283.5 35 48375.5 36 53246.0 37 58566.0 38 59943.5 39 59582.5 40 59564.5 41 61039.0 42 62252.5 43 64069.5 44 67621.0 45 70420.5 46 72054.5 47 82424.0 48 110533.5 49 107424.5 50 78589.5 51 67092.0 52 62513.5 53 61362.0 54 59738.5 55 56214.0 56 50827.5 57 45854.5 58 42676.0 59 38594.0 60 33049.5 61 27294.5 62 21969.5 63 16850.0 64 12540.0 65 9658.5 66 7193.5 67 5638.5 68 4365.5 69 3051.5 70 2212.5 71 1644.0 72 1321.5 73 1072.5 74 809.0 75 618.5 76 438.0 77 278.0 78 182.0 79 121.0 80 85.5 81 63.5 82 53.0 83 44.5 84 27.5 85 20.0 86 17.0 87 13.0 88 12.5 89 12.0 90 7.5 91 6.5 92 8.5 93 5.5 94 4.5 95 5.0 96 2.5 97 2.5 98 2.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33648301283742527 2 0.1182072079542285 3 0.11208248215349129 4 0.11919658673742453 5 0.1351679870947316 6 0.08664131344273664 7 0.09559283576689105 8 0.10105797571216427 9 0.08975078961849554 10-11 0.08812538161753067 12-13 0.09154109408332642 14-15 0.09304871889581559 16-17 0.08233044874452544 18-19 0.09698267739090449 20-21 0.1060990961789249 22-23 0.11347232377750473 24-25 0.12852501526470123 26-27 0.12711161700299264 28-29 0.12174070360849998 30-31 0.11212959542888157 32-33 0.10555729351193661 34-35 0.3264478851792943 36-37 0.4861147754769748 38-39 0.4600140209107561 40-41 0.43923706646363986 42-43 0.47134476364212 44-45 0.4786237646899193 46-47 0.48557297280998646 48-49 0.4950191845257389 50-51 0.48465426393987593 52-53 0.48832909942031827 54-55 0.4754907318764652 56-57 0.4540070782984946 58-59 0.4339603796199277 60-61 0.39561017345223465 62-63 0.46401864931893044 64-65 0.4936528995394206 66-67 0.5019919492835012 68-69 0.5161730451759775 70-71 0.5073864193156891 72-73 0.49892958638313273 74-75 0.5062085874309319 76-77 0.4832879789535576 78-79 0.4625345811441365 80-81 0.46484313163826046 82-83 0.4776343859067233 84-85 0.4768334602250884 86-87 0.48017850277779867 88-89 0.4923337278284926 90-91 0.50752775914186 92-93 0.5005549943840976 94-95 0.5051485387346505 96-97 0.5051249820969553 98-99 0.499306492586255 100-101 0.4863974551293165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.569835064353214 #Duplication Level Percentage of deduplicated Percentage of total 1 69.74018314393396 30.385682769389888 2 14.838041010486219 12.929819990099869 3 5.935967740934712 7.758874062595403 4 3.085642948556283 5.3776381734432706 5 1.7297032029770318 3.7681441631996373 6 1.0398556208412602 2.718380273447659 7 0.6962134783633894 2.1233734495310763 8 0.4785801880170362 1.6681327885575536 9 0.37443288931914515 1.4682581307273939 >10 1.8628945154514773 14.863117113786291 >50 0.13923364501998464 4.193419684821941 >100 0.07188123344149064 5.665409396302864 >500 0.0032516394079369917 0.9070402844820373 >1k 0.0034684153684661216 3.2445095905166794 >5k 3.251639407936989E-4 1.0526333077057637 >10k+ 3.251639407936989E-4 1.8755668213926473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15359 0.7236127967194084 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13282 0.6257585237337836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11075 0.5217795249474215 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8737 0.4116286870849321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7620 0.3590031584739822 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5933 0.27952306289056905 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4735 0.223081358973006 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4059 0.19123278480917238 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3785 0.1783237473522339 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3566 0.1680059400417612 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3493 0.1645666709382703 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3365 0.15853617168831363 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3084 0.1452973413036432 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2978 0.14030333411227283 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2837 0.13366036228224246 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2806 0.13219985074514357 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2454 0.11561597780776277 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2429 0.1144381459230056 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2209 0.1040732253371426 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2123 0.10002148365357798 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 2.3556637695143187E-5 0.0 0.0 0.0 0.0 18-19 4.7113275390286375E-5 0.0 0.0 0.0 0.0 20-21 4.7113275390286375E-5 0.0 0.0 4.7113275390286375E-5 0.0 22-23 7.066991308542957E-5 0.0 0.0 1.648964638660023E-4 0.0 24-25 9.422655078057275E-5 0.0 0.0 8.009256816348684E-4 0.0 26-27 9.422655078057275E-5 0.0 0.0 0.0015547380878794502 0.0 28-29 9.422655078057275E-5 0.0 0.0 0.0049468939159800695 0.0 30-31 9.422655078057275E-5 0.0 0.0 0.014958464936415923 0.0 32-33 9.422655078057275E-5 0.0 0.0 0.03133032813454044 0.0 34-35 1.4133982617085914E-4 0.0 0.0 0.05483985255429334 4.7113275390286375E-5 36-37 1.884531015611455E-4 0.0 0.0 0.09066949848860612 4.7113275390286375E-5 38-39 1.884531015611455E-4 0.0 0.0 0.14628672008683918 4.7113275390286375E-5 40-41 1.884531015611455E-4 0.0 0.0 0.21231597554632553 4.7113275390286375E-5 42-43 2.3556637695143186E-4 0.0 0.0 0.277874098251909 4.7113275390286375E-5 44-45 2.3556637695143186E-4 0.0 0.0 0.35784888322692016 4.7113275390286375E-5 46-47 2.3556637695143186E-4 0.0 0.0 0.4602495872877076 4.7113275390286375E-5 48-49 2.3556637695143186E-4 0.0 0.0 0.5666784763943645 4.7113275390286375E-5 50-51 2.3556637695143186E-4 0.0 0.0 0.6830011533329816 4.7113275390286375E-5 52-53 2.3556637695143186E-4 0.0 0.0 0.798240224937622 4.7113275390286375E-5 54-55 2.3556637695143186E-4 0.0 0.0 0.9303222924942899 4.7113275390286375E-5 56-57 2.3556637695143186E-4 0.0 0.0 1.0950538598964261 4.7113275390286375E-5 58-59 2.3556637695143186E-4 0.0 0.0 1.2739900798287338 4.7113275390286375E-5 60-61 2.3556637695143186E-4 0.0 0.0 1.4826547765323124 4.7113275390286375E-5 62-63 2.3556637695143186E-4 0.0 0.0 1.6913194732358905 4.7113275390286375E-5 64-65 2.826796523417182E-4 0.0 0.0 1.9021278239697268 4.7113275390286375E-5 66-67 2.826796523417182E-4 0.0 0.0 2.153359364988429 4.7113275390286375E-5 68-69 2.826796523417182E-4 0.0 0.0 2.437805765157283 4.7113275390286375E-5 70-71 2.826796523417182E-4 0.0 0.0 2.757657791781937 4.7113275390286375E-5 72-73 2.826796523417182E-4 0.0 0.0 3.088911231051041 4.7113275390286375E-5 74-75 2.826796523417182E-4 0.0 0.0 3.432013659080801 4.7113275390286375E-5 76-77 2.826796523417182E-4 0.0 0.0 3.7928306786573094 4.7113275390286375E-5 78-79 2.826796523417182E-4 0.0 0.0 4.220265869635682 4.7113275390286375E-5 80-81 4.711327539028637E-4 0.0 0.0 4.695803714787538 4.7113275390286375E-5 82-83 4.711327539028637E-4 0.0 0.0 5.183638124816258 4.7113275390286375E-5 84-85 4.711327539028637E-4 0.0 0.0 5.686430999781394 4.7113275390286375E-5 86-87 4.711327539028637E-4 0.0 0.0 6.216054885081299 4.7113275390286375E-5 88-89 4.711327539028637E-4 0.0 0.0 6.781037283561613 4.7113275390286375E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 10650 0.0 23.16815 12-13 GTACATG 15135 0.0 22.237823 1 AGTACTT 11105 0.0 21.984335 12-13 TACATGG 14995 0.0 21.92756 2 GTACTTT 11495 0.0 21.365078 14-15 ACATGGG 15205 0.0 20.72109 3 GTATCGC 115 0.0037884426 20.591389 6 GTGTAGC 1065 0.0 20.058176 1 CATGGGA 10000 0.0 18.951227 4 ACTTTTT 13345 0.0 18.831404 16-17 ATGGGAT 3760 0.0 18.648813 5 TACTTTT 13125 0.0 18.531298 14-15 GTATAGG 720 0.0 18.460949 1 TAGCGCG 155 9.795808E-4 18.339897 4 GTAAGCG 160 8.163624E-9 17.834509 94-95 AGAGTAC 23885 0.0 17.537003 10-11 CATGGGG 4915 0.0 17.351074 4 TACCTGG 1440 0.0 17.108747 2 GTATCAA 34000 0.0 16.880133 1 TATCGCT 200 2.8245035E-4 16.576067 7 >>END_MODULE