FastQCFastQC Report
Fri 27 May 2016
SRR939177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939177_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122544
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149990.7066520175789053No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146350.6895027853368411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114530.5395883430449498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80370.37864939431173156No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79750.37572837123753383No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56180.2646823811426289No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44480.20955984893599378No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43150.2032937833090857No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40500.19080876533065982No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39180.184589812979142No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37440.17639210306123218No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33850.15947843719611937No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33180.15632184774497018No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31850.1500557821180621No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31190.14694630594230318No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.12706450372760236No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26930.1268760506260412No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25600.12060998499913311No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT22320.1051568306711192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21600.10176467484301857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT118900.023.4447112-13
TATTCCG3150.022.5938015
TTTACGC1703.18176E-622.3279933
GTACTTT124100.022.25205214-15
AGTACTT122200.021.72439812-13
TACATGG140850.020.9191192
GTACATG143350.020.8235231
GTAAGCG2400.020.75903394-95
CCGTTGC1908.3367195E-619.9776789
ACATGGG144150.019.913583
GGGTACG1200.00479568319.7695777
CCTATAC5050.019.7304293
CGCCGAA1456.1947986E-419.6332347
ACTTTTT143700.019.41486216-17
TACTTTT139300.018.93836814-15
CATGGGG46200.018.5885374
CATGGGA94950.017.6395854
ATAGTAC4900.017.4295043
AGAGTAC252200.017.35562110-11
ATGGGAT35650.017.3018465
Produced by FastQC (version 0.11.5)