##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939177_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2122544 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.500456998771284 34.0 31.0 34.0 31.0 34.0 2 32.69425133236343 34.0 31.0 34.0 31.0 34.0 3 32.77561077650216 34.0 31.0 34.0 31.0 34.0 4 36.237235129165754 37.0 35.0 37.0 35.0 37.0 5 36.208567643356275 37.0 35.0 37.0 35.0 37.0 6 36.263224696402055 37.0 37.0 37.0 35.0 37.0 7 36.22068800458318 37.0 37.0 37.0 35.0 37.0 8 36.21702306289057 37.0 37.0 37.0 35.0 37.0 9 37.94658014156597 39.0 38.0 39.0 35.0 39.0 10-11 37.890925464913806 39.0 38.0 39.0 35.0 39.0 12-13 37.83186120052164 39.0 38.0 39.0 35.0 39.0 14-15 39.14230352821897 40.5 38.5 41.0 36.0 41.0 16-17 39.10915180085784 40.0 38.5 41.0 36.0 41.0 18-19 39.19512198569264 40.5 38.5 41.0 36.0 41.0 20-21 39.27309327863168 41.0 39.0 41.0 36.0 41.0 22-23 39.22473574163834 41.0 39.0 41.0 36.0 41.0 24-25 39.16088147053724 41.0 39.0 41.0 35.5 41.0 26-27 39.007063693379266 40.5 38.5 41.0 35.0 41.0 28-29 38.859601968204196 40.0 38.0 41.0 35.0 41.0 30-31 38.64500264776608 40.0 38.0 41.0 35.0 41.0 32-33 38.449874772914015 40.0 38.0 41.0 34.0 41.0 34-35 38.36249543001229 40.0 38.0 41.0 34.0 41.0 36-37 38.23328515215704 40.0 38.0 41.0 34.0 41.0 38-39 38.07985629508741 40.0 38.0 41.0 34.0 41.0 40-41 37.90532869989974 40.0 37.5 41.0 33.0 41.0 42-43 37.692807310472716 40.0 37.0 41.0 33.0 41.0 44-45 37.38980511122502 40.0 37.0 41.0 33.0 41.0 46-47 36.97558354502898 40.0 37.0 41.0 31.5 41.0 48-49 36.55813095040668 40.0 36.0 41.0 30.5 41.0 50-51 36.26972585727316 40.0 36.0 41.0 30.0 41.0 52-53 36.036960600110056 39.5 35.5 41.0 29.5 41.0 54-55 35.80284177854499 39.0 35.0 41.0 28.5 41.0 56-57 35.526316062234756 39.0 35.0 40.5 28.0 41.0 58-59 35.24130760069049 38.5 35.0 40.0 27.5 41.0 60-61 34.86752712782397 38.0 35.0 40.0 26.5 41.0 62-63 34.49184445646357 37.5 34.0 40.0 26.0 41.0 64-65 34.088199113893516 37.0 34.0 40.0 26.0 41.0 66-67 33.71710574668888 36.5 34.0 39.0 25.0 41.0 68-69 33.247221494583854 36.0 33.0 39.0 23.5 40.0 70-71 32.8101125347696 35.5 33.0 38.5 22.5 40.0 72-73 32.35253733255942 35.0 32.5 37.5 21.0 40.0 74-75 31.8776767407413 35.0 32.0 37.0 19.5 39.0 76-77 29.912601811788118 33.5 29.5 35.0 14.0 37.5 78-79 30.869016378459058 34.5 31.0 36.0 16.0 38.0 80-81 30.893533655839406 35.0 31.0 36.0 16.0 37.0 82-83 30.60943683617395 35.0 31.0 35.0 10.0 37.0 84-85 30.247754345728524 34.0 31.0 35.0 6.5 36.5 86-87 29.734065583563876 34.0 30.5 35.0 2.0 36.0 88-89 29.207638569565578 34.0 30.0 35.0 2.0 36.0 90-91 28.77240259801446 34.0 29.0 35.0 2.0 35.0 92-93 28.522070920555713 34.0 29.0 35.0 2.0 35.0 94-95 28.22834179173671 34.0 29.0 35.0 2.0 35.0 96-97 27.712006441327013 34.0 29.0 35.0 2.0 35.0 98-99 27.014489687846282 34.0 27.0 35.0 2.0 35.0 100-101 23.508796519648122 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1826928361500535 1101 2 0.17812533131752417 1101 3 0.2477790012348251 1101 4 0.17536921480770218 1101 5 0.13422555807728287 1101 6 0.12086436272570467 1101 7 0.1826906799639758 1101 8 0.2315854209669297 1101 9 0.27917290950141194 1101 10-11 0.28743645803427853 1101 12-13 0.25454295284838224 1101 14-15 0.3200199347754875 1101 16-17 0.2959279959740968 1101 18-19 0.3798020520025247 1101 20-21 0.4025491529029708 1101 22-23 0.3965530779203732 1101 24-25 0.3091519689050912 1101 26-27 0.3699555280136835 1101 28-29 0.3465992344901778 1101 30-31 0.3123418195197871 1101 32-33 0.348281007588497 1101 34-35 0.5523585003094027 1101 36-37 0.49652065645672394 1101 38-39 0.5879207507977569 1101 40-41 0.6713896755877897 1101 42-43 0.6551904055835607 1101 44-45 0.7879651521208615 1101 46-47 0.6574637520744844 1101 48-49 0.5211956284859767 1101 50-51 0.47376178594169716 1101 52-53 0.39443702929668234 1101 54-55 0.3694338911115551 1101 56-57 0.46931007936900215 1101 58-59 0.5563712695352621 1101 60-61 0.5214194379693069 1101 62-63 0.35450291773356923 1101 64-65 0.3448951352489473 1101 66-67 0.34820976189440245 1101 68-69 0.2457497715719157 1101 70-71 0.22371760359020954 1101 72-73 0.3301268052394519 1101 74-75 0.3552633581493545 1101 76-77 0.2885879598785195 1101 78-79 0.42115350924207107 1101 80-81 0.30472662024282116 1101 82-83 0.32288338828908536 1101 84-85 0.2133724007779847 1101 86-87 0.28302756678345986 1101 88-89 0.43781557444647845 1101 90-91 0.48875113109601287 1101 92-93 0.3058986684790703 1101 94-95 0.2627428354708776 1101 96-97 0.04563795536136794 1101 98-99 -0.014471957194718499 1101 100-101 -0.4317230913117349 1102 1 0.05300119126435732 1102 2 0.04107986679774456 1102 3 0.11792491340225553 1102 4 0.03414381592340732 1102 5 0.03100662765284312 1102 6 0.049048122575904074 1102 7 0.10879620631569509 1102 8 0.152525383516668 1102 9 0.25974314824682665 1102 10-11 0.16771813959002912 1102 12-13 0.18529212265215733 1102 14-15 0.2180920143377918 1102 16-17 0.24678199481353147 1102 18-19 0.22984587807336965 1102 20-21 0.16612685366172997 1102 22-23 0.16470295332997864 1102 24-25 0.16799605781044846 1102 26-27 0.20835085380169716 1102 28-29 0.15837387949290616 1102 30-31 0.20706598956738276 1102 32-33 0.17514958911107925 1102 34-35 0.16806024164903732 1102 36-37 0.43021650474415196 1102 38-39 0.29566638403876766 1102 40-41 0.37340881398795744 1102 42-43 0.24103373134231987 1102 44-45 0.1805405341817945 1102 46-47 0.23500084880148364 1102 48-49 0.3566116652925686 1102 50-51 0.41578595915890304 1102 52-53 0.49934621972736437 1102 54-55 0.35841687635576847 1102 56-57 0.4764447686488609 1102 58-59 0.32242706335402005 1102 60-61 0.4300359848406501 1102 62-63 0.35705217482376383 1102 64-65 0.3713008000886191 1102 66-67 0.3128974691386901 1102 68-69 0.3280926499268304 1102 70-71 0.2585032520453794 1102 72-73 0.19720519721885665 1102 74-75 0.2638776192381478 1102 76-77 0.3497803398718844 1102 78-79 0.5275853356250018 1102 80-81 0.4488983928397623 1102 82-83 0.45057604013818775 1102 84-85 0.5936999503703255 1102 86-87 0.7952962760660505 1102 88-89 0.7175406527116763 1102 90-91 0.8814310496124378 1102 92-93 0.5882251196766966 1102 94-95 0.6384985151833718 1102 96-97 0.43689306941887196 1102 98-99 0.532519160559044 1102 100-101 0.21961019041370733 1103 1 0.1047307092556764 1103 2 0.1198501154355327 1103 3 0.12880320261569977 1103 4 0.04739963541697989 1103 5 0.05381462502474221 1103 6 0.08380141557591969 1103 7 0.12821142915622374 1103 8 0.15371885430795373 1103 9 0.09771904766588335 1103 10-11 0.16716699019060144 1103 12-13 0.1448694022387116 1103 14-15 0.22422921289303588 1103 16-17 0.2785286707856187 1103 18-19 0.26629482308106844 1103 20-21 0.22045506754063382 1103 22-23 0.23040913776151228 1103 24-25 0.17887331766268488 1103 26-27 0.27544991099506433 1103 28-29 0.3392032328746666 1103 30-31 0.23734626697728345 1103 32-33 0.2859909095521118 1103 34-35 0.23226326461820435 1103 36-37 0.3019389819048399 1103 38-39 0.28957418327333784 1103 40-41 0.4281631834057009 1103 42-43 0.2692445515755608 1103 44-45 0.2675069978878426 1103 46-47 0.2066204780726295 1103 48-49 0.2842065451911182 1103 50-51 0.16138183724371657 1103 52-53 0.17884622663878957 1103 54-55 0.15181393597077175 1103 56-57 0.1923074012783772 1103 58-59 0.13781818973104265 1103 60-61 0.10675142264120296 1103 62-63 0.12776417220506886 1103 64-65 -0.018371089556801223 1103 66-67 -0.029780552264377036 1103 68-69 0.027180224154804478 1103 70-71 -0.10486566483428561 1103 72-73 -0.07660400097640974 1103 74-75 -0.19753442317035663 1103 76-77 -0.018409663736761672 1103 78-79 0.10485183459402236 1103 80-81 0.1200905725179986 1103 82-83 0.17794104897451746 1103 84-85 0.09057554501023546 1103 86-87 0.25930113229811624 1103 88-89 0.1393741979463634 1103 90-91 0.40294690456897087 1103 92-93 0.2554945508546389 1103 94-95 0.017013746935219842 1103 96-97 0.13824979689725936 1103 98-99 0.11929283961657333 1103 100-101 -0.43756473658252304 1104 1 0.013026232806588212 1104 2 0.004818435420872902 1104 3 0.10949300199470002 1104 4 -0.025385914371632623 1104 5 0.07274901932348143 1104 6 0.05067059372873928 1104 7 0.047168738474617555 1104 8 0.01293040576936022 1104 9 0.1267223015986758 1104 10-11 0.019147134326075843 1104 12-13 0.058692601404608524 1104 14-15 -0.005599841060373478 1104 16-17 0.07208324863367466 1104 18-19 0.05470469258003163 1104 20-21 0.06595269583610985 1104 22-23 0.14784130930335238 1104 24-25 0.0993637850145106 1104 26-27 0.03518277915350154 1104 28-29 -0.03245661657364707 1104 30-31 0.08211290614593025 1104 32-33 0.0829397746857623 1104 34-35 0.06308940836411381 1104 36-37 0.029513627581351898 1104 38-39 0.2566351314968429 1104 40-41 0.2578863986121007 1104 42-43 0.30601914798477736 1104 44-45 0.184444777880735 1104 46-47 0.2426437482750856 1104 48-49 0.16111536632792678 1104 50-51 0.12739614194473603 1104 52-53 0.21416299814167417 1104 54-55 0.24233828890790932 1104 56-57 0.4138627009495437 1104 58-59 0.3230589120124634 1104 60-61 0.40681314997538465 1104 62-63 0.23530292723204838 1104 64-65 0.2177223578933294 1104 66-67 0.3964121745083844 1104 68-69 0.2524497620734394 1104 70-71 0.15412461346862472 1104 72-73 0.08622482955556166 1104 74-75 0.08615122859009361 1104 76-77 0.15509513123109997 1104 78-79 0.22401967565544467 1104 80-81 0.21032003779601283 1104 82-83 0.21879666716446167 1104 84-85 0.13895774462296728 1104 86-87 0.1737986059171952 1104 88-89 0.2606748544616764 1104 90-91 0.3420957834516365 1104 92-93 0.10723795419335502 1104 94-95 0.19146980165628946 1104 96-97 0.3491179610604611 1104 98-99 0.3166189432612079 1104 100-101 0.077674467578845 1105 1 0.042868942635593044 1105 2 0.09894017757811469 1105 3 0.09010367074986902 1105 4 0.08092309663650354 1105 5 0.06477464696130397 1105 6 0.12333843517676968 1105 7 0.12924135736683695 1105 8 0.06610557872328116 1105 9 0.1159480336615033 1105 10-11 0.10318424871496745 1105 12-13 0.11825727964505006 1105 14-15 0.19223660326804293 1105 16-17 0.11830545635241663 1105 18-19 0.09242278856401498 1105 20-21 0.16319576680159997 1105 22-23 0.13438299228642592 1105 24-25 0.024991966714026148 1105 26-27 0.11031657369728975 1105 28-29 0.1853326189482587 1105 30-31 0.1628590010856712 1105 32-33 0.1528914781187325 1105 34-35 0.20050728244495986 1105 36-37 0.1661292782796906 1105 38-39 0.08248304082112412 1105 40-41 0.09170868029856649 1105 42-43 0.2467938510261547 1105 44-45 0.1739255897851919 1105 46-47 0.03652291983667766 1105 48-49 0.07006654763585374 1105 50-51 0.09190958666474813 1105 52-53 0.07781167324935723 1105 54-55 0.14327593847497866 1105 56-57 0.11218319870875604 1105 58-59 0.12122494777803183 1105 60-61 0.18847296375077605 1105 62-63 0.14643833166140752 1105 64-65 -0.15506948825382239 1105 66-67 0.06626706725520393 1105 68-69 -0.04835844399191558 1105 70-71 -0.02490499537269386 1105 72-73 -0.0038912344917818587 1105 74-75 -0.02256390868528868 1105 76-77 0.10639500505088506 1105 78-79 -0.07076124937609762 1105 80-81 0.019686199741798305 1105 82-83 -0.07978217371792695 1105 84-85 -0.09292016513641599 1105 86-87 0.1356519322888623 1105 88-89 0.19252121916602505 1105 90-91 0.15410030853539425 1105 92-93 0.1881428621688137 1105 94-95 -0.16587366960507666 1105 96-97 -0.15529997828601338 1105 98-99 -0.13632344894172022 1105 100-101 -0.6716755955112639 1106 1 0.10960754361595804 1106 2 0.0829805224517699 1106 3 0.11865851038773201 1106 4 0.06069876434108323 1106 5 0.026664875198605387 1106 6 0.09087961757136043 1106 7 0.11698323649663678 1106 8 0.11642928313491296 1106 9 0.16479297411017058 1106 10-11 0.08612297095603338 1106 12-13 0.14608969324680032 1106 14-15 0.1664412152851824 1106 16-17 0.1689238345419355 1106 18-19 0.14755992619491565 1106 20-21 0.13769839240404735 1106 22-23 0.17441700694047313 1106 24-25 0.15398663329848716 1106 26-27 0.18693355430585967 1106 28-29 0.15066644554545405 1106 30-31 0.20191804888087717 1106 32-33 0.2364627085037867 1106 34-35 0.13471615913927337 1106 36-37 0.23982983628443577 1106 38-39 0.32600458514808395 1106 40-41 0.3400485391017867 1106 42-43 0.4126052700846401 1106 44-45 0.386889180746131 1106 46-47 0.3179217339481326 1106 48-49 0.5133365787731279 1106 50-51 0.5503312964406177 1106 52-53 0.722108309806373 1106 54-55 0.673459319593249 1106 56-57 0.6256958026281509 1106 58-59 0.5640163588209788 1106 60-61 0.503374413741021 1106 62-63 0.6372461540042096 1106 64-65 0.7664072718246828 1106 66-67 0.6873535836819116 1106 68-69 0.5533959825374595 1106 70-71 0.8602737100151856 1106 72-73 0.7519457212480702 1106 74-75 0.6522637058701086 1106 76-77 0.7340403894370269 1106 78-79 0.8811403855880862 1106 80-81 0.6864653922277455 1106 82-83 0.9236814634864992 1106 84-85 0.9261115990012421 1106 86-87 0.9458851022285408 1106 88-89 1.05154409427778 1106 90-91 1.2425314725353545 1106 92-93 1.125014336102904 1106 94-95 0.838064477161474 1106 96-97 0.9777838971237536 1106 98-99 0.9023695045784343 1106 100-101 0.6848606645210005 1107 1 0.01630820523278942 1107 2 -0.04151412383769326 1107 3 0.027061884188888996 1107 4 0.03382348331451368 1107 5 0.00795529978960019 1107 6 0.029344310752108527 1107 7 0.07543972740743499 1107 8 0.04761470878825236 1107 9 0.13884860131205556 1107 10-11 0.10341197250260592 1107 12-13 0.0366612827577768 1107 14-15 0.1210502980638779 1107 16-17 0.18855221241952336 1107 18-19 0.08297149732489828 1107 20-21 0.15060535593359958 1107 22-23 0.05444892661752476 1107 24-25 0.004712484778103487 1107 26-27 0.08964573862964187 1107 28-29 0.12672767598477463 1107 30-31 0.17374374570324136 1107 32-33 0.219131954438474 1107 34-35 0.19123696375230992 1107 36-37 0.08056621236345052 1107 38-39 0.14108119731412927 1107 40-41 0.1421065182798813 1107 42-43 0.13873740568891435 1107 44-45 0.012424181215536123 1107 46-47 0.13085636778595955 1107 48-49 0.157323969018357 1107 50-51 0.10566425424833881 1107 52-53 0.13576360694101908 1107 54-55 0.13875087076108628 1107 56-57 0.2132073525112972 1107 58-59 0.19705470690357885 1107 60-61 0.24222105599852028 1107 62-63 0.19366764197503272 1107 64-65 0.23932322820441954 1107 66-67 0.19762297389749506 1107 68-69 0.04037314959199989 1107 70-71 0.03472018032149293 1107 72-73 0.10859375986790809 1107 74-75 0.14106241920077522 1107 76-77 0.07089724006183218 1107 78-79 0.3526029096143155 1107 80-81 0.2766626914080099 1107 82-83 0.1643271698946478 1107 84-85 0.3520898185157293 1107 86-87 0.2786820762826885 1107 88-89 0.3478733314172615 1107 90-91 0.4378252966213694 1107 92-93 0.5205245815286546 1107 94-95 0.3960483493246514 1107 96-97 0.44816579678923674 1107 98-99 0.5146528979464513 1107 100-101 0.2815686390050267 1108 1 0.007638367592299744 1108 2 0.03032489984110498 1108 3 0.025544615554714767 1108 4 0.028614900043514524 1108 5 0.028040172738933222 1108 6 0.016365900661448052 1108 7 0.073812839054888 1108 8 0.05182636423418785 1108 9 0.042728104306604564 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-0.07112130493435842 2316 84-85 -0.138062752610292 2316 86-87 -0.2808990014399839 2316 88-89 -0.4129671064143281 2316 90-91 -0.47066822558896604 2316 92-93 -0.292166807711407 2316 94-95 -0.047658091726923146 2316 96-97 -0.30063751396097516 2316 98-99 -0.3548193701983813 2316 100-101 0.052850798340283944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 26.0 6 104.0 7 223.0 8 477.0 9 967.0 10 1801.0 11 3007.0 12 4619.0 13 6852.0 14 8315.0 15 11818.0 16 16466.0 17 25478.0 18 24804.0 19 14079.0 20 11213.0 21 11599.0 22 11787.0 23 12341.0 24 14124.0 25 15891.0 26 18752.0 27 22572.0 28 27027.0 29 33213.0 30 40489.0 31 49575.0 32 62643.0 33 81349.0 34 114598.0 35 172813.0 36 284107.0 37 498008.0 38 471884.0 39 49511.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4534508695607 19.92259855541257 15.7367651188584 20.887185456168336 2 15.042656359538364 21.952100875176203 39.75950557444274 23.245737190842686 3 19.642466775718194 26.496741645874007 30.602663596137464 23.25812798227033 4 12.550175638290655 17.859323528746636 38.15807823065152 31.43242260231119 5 12.821312538161752 38.93026481429832 33.56462810664938 14.683794540890554 6 29.33654143329891 37.584474102774784 17.51440723961435 15.564577224311957 7 26.845474110312907 34.71942159974069 20.93633865776163 17.498765632184774 8 26.045019561431943 33.551907522294 19.93913907085083 20.463933845423227 9 26.650095357269386 17.56378195222337 21.23084374222631 34.55527894828093 10-11 24.705141565969893 27.2160671345329 26.635184005608366 21.443607293888842 12-13 26.853035791013045 25.08094060712051 27.242544795302244 20.8234788065642 14-15 23.295095838727853 25.93716254798532 27.5874147948386 23.180326818448226 16-17 20.76379798177772 29.341866934525445 27.931862795560825 21.962472288136013 18-19 20.770876834590943 27.916712209499543 30.796252044716148 20.51615891119336 20-21 22.55189998416994 26.082356832178743 30.406955050166218 20.9587881334851 22-23 21.78908894232581 26.254131834251726 30.53745882299731 21.41932040042515 24-25 21.947997981656822 26.5204828381938 30.735046286677285 20.796472893472092 26-27 21.577999390348186 26.19043468551342 31.454476582821485 20.777089341316916 28-29 20.766071280501134 27.332743161036944 31.056340881508227 20.844844676953695 30-31 21.240717563031524 26.565855802820227 30.70806710704576 21.485359527102492 32-33 21.237062694577826 26.66286305490016 30.773779012354986 21.326295238167027 34-35 22.04327876762093 26.454224452890074 30.45689321284963 21.04560356663937 36-37 21.9402524014757 26.094893133830656 30.983943507385685 20.980910957307962 38-39 21.48780289545019 26.737664767231863 30.79508840795006 20.979443929367886 40-41 21.415606873771953 26.887907759144692 30.615967808054585 21.080517559028774 42-43 21.15907216366121 27.050553505795854 29.732457474324864 22.057916856218068 44-45 21.632610754349844 26.650420025536043 29.720705397905288 21.99626382220882 46-47 22.21345905184852 27.22914913149208 29.184466856488246 21.372924960171154 48-49 22.002746250289455 27.11091784075659 29.09256192078276 21.793773988171196 50-51 21.50535101433824 27.568012215544428 29.359920642305465 21.566716127811873 52-53 21.869657794999252 28.046515890015915 28.294567343378358 21.789258971606472 54-55 21.33041764976368 27.43123346324034 28.70666049796848 22.531688389027508 56-57 21.991770253054824 27.562231925463028 28.235268621050963 22.21072920043118 58-59 22.503394642636714 27.122652843725824 28.360931785822313 22.01302072781515 60-61 21.877442764798648 27.40358632621838 29.21425370781953 21.504717201163434 62-63 21.553527510530234 27.55088435117741 29.079440837518728 21.81614730077363 64-65 21.33767977296622 27.949965689102513 28.70574771725936 22.00660682067191 66-67 21.547967136464333 27.470513184142657 28.218084179123043 22.763435500269967 68-69 21.321925606348614 27.545542842698833 28.58986388325541 22.542667667697145 70-71 21.932171959686112 27.563268417521613 28.212159559472028 22.29240006332024 72-73 21.679597690318786 27.0676367604158 28.739734017292456 22.513031531972956 74-75 21.197016410496083 28.48553433992417 27.910540370423416 22.406908879156333 76-77 21.452241749914126 27.370560676508816 28.267260642159275 22.909936931417786 78-79 21.384244420709745 27.504608871364788 28.464380126235728 22.646766581689743 80-81 21.608206641676986 27.65611955093956 28.12114247699385 22.6145313303896 82-83 21.35949957856917 27.386431576264375 28.49755786841276 22.756510976753695 84-85 21.192493536058617 27.43903071031743 28.301533442887404 23.06694231073655 86-87 21.128123713265726 27.650572377137365 28.15123434517518 23.07006956442173 88-89 21.135342402504985 27.364715193428907 27.870684237329307 23.6292581667368 90-91 21.10002568363158 26.893860382265146 28.32180880877614 23.684305125327135 92-93 21.324976066456102 27.303320920555713 28.249779509871175 23.121923503117014 94-95 20.887152398254173 27.03171288793071 28.394841284797867 23.686293429017255 96-97 20.425371629516277 26.91739252519618 28.754504029127308 23.90273181616023 98-99 20.59832251088625 27.193368252558574 27.90714328621554 24.30116595033963 100-101 20.63137963177374 26.174561307008414 28.759004893460695 24.43505416775715 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1637.0 1 1087.0 2 657.5 3 824.0 4 730.5 5 710.5 6 1295.0 7 2822.0 8 4502.0 9 5111.5 10 4503.5 11 3869.5 12 3976.5 13 4129.0 14 4212.0 15 4344.5 16 4468.0 17 4648.0 18 4902.0 19 5192.5 20 5594.5 21 6185.0 22 7084.5 23 8379.0 24 9545.5 25 11305.0 26 14436.0 27 17616.0 28 19767.0 29 22546.5 30 26524.0 31 30750.0 32 35494.5 33 39867.5 34 44411.5 35 48493.0 36 53550.0 37 58835.5 38 59995.0 39 59870.0 40 60108.0 41 61283.0 42 62602.5 43 64784.5 44 68431.5 45 71008.0 46 72178.5 47 82883.5 48 111162.0 49 108107.5 50 80253.5 51 68620.5 52 63041.0 53 61782.5 54 60401.0 55 56360.0 56 50860.5 57 46067.0 58 43055.5 59 39094.0 60 32964.5 61 27020.5 62 21858.5 63 16871.5 64 12389.0 65 9258.0 66 6910.0 67 5349.5 68 4017.5 69 2809.0 70 2045.5 71 1497.5 72 1204.5 73 1002.5 74 760.5 75 564.0 76 373.5 77 203.5 78 114.5 79 74.0 80 44.0 81 28.0 82 17.0 83 12.0 84 7.5 85 2.5 86 1.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3457172148139214 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.422655078057274E-4 16-17 0.001531181450184307 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.182460292931501E-4 26-27 9.893787831960139E-4 28-29 0.0 30-31 0.0025676735087706076 32-33 0.0 34-35 0.009893787831960137 36-37 0.026736783783987517 38-39 0.019858245577005707 40-41 0.012909037456938464 42-43 0.00982311791887471 44-45 0.0025441168710754643 46-47 0.0011542752470620162 48-49 6.360292177688661E-4 50-51 1.1778318847571593E-4 52-53 2.3556637695143187E-5 54-55 0.0 56-57 0.0 58-59 0.003863288582003483 60-61 0.009658221455008706 62-63 0.0039575151327840554 64-65 0.0019080876533065982 66-67 0.003297929277320046 68-69 0.0030388062626734713 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21726286946230558 78-79 3.2979292773200464E-4 80-81 0.0032037027265394732 82-83 6.124725800737229E-4 84-85 0.0 86-87 0.11076331044256327 88-89 0.40891967374999055 90-91 0.3937256424366232 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012485017978425888 100-101 8.244823193300115E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2122544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.27713051142447 #Duplication Level Percentage of deduplicated Percentage of total 1 68.35226627445181 28.897376820366365 2 15.4136087830369 13.032863003449787 3 6.293761891390506 7.982465786704383 4 3.2070380009552597 5.4233745648593334 5 1.7944622080412624 3.793235648358969 6 1.1025467099774189 2.7967506691594197 7 0.7446735255408331 2.2037861879384666 8 0.5034023384331999 1.7025925089357332 9 0.39787323884401227 1.5138844961050317 >10 1.9546414112348156 15.070540252356412 >50 0.15083183237377157 4.381598114880261 >100 0.07697525168852765 5.900795185096115 >500 0.0036804453949931277 1.0242164594999725 >1k 0.0035689167466600013 3.3193439976005457 >5k 3.345859449993751E-4 1.0198785571763374 >10k+ 3.345859449993751E-4 1.9372977475129067 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14999 0.7066520175789053 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14635 0.6895027853368411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11453 0.5395883430449498 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8037 0.37864939431173156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7975 0.37572837123753383 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5618 0.2646823811426289 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4448 0.20955984893599378 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4315 0.2032937833090857 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4050 0.19080876533065982 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3918 0.184589812979142 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3744 0.17639210306123218 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3385 0.15947843719611937 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3318 0.15632184774497018 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3185 0.1500557821180621 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3119 0.14694630594230318 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2697 0.12706450372760236 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2693 0.1268760506260412 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2560 0.12060998499913311 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2232 0.1051568306711192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2160 0.10176467484301857 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.3556637695143187E-5 0.0 0.0 0.0 0.0 14-15 4.7113275390286375E-5 0.0 0.0 4.7113275390286375E-5 0.0 16-17 4.7113275390286375E-5 0.0 0.0 4.7113275390286375E-5 0.0 18-19 4.7113275390286375E-5 0.0 0.0 4.7113275390286375E-5 0.0 20-21 4.7113275390286375E-5 0.0 0.0 4.7113275390286375E-5 0.0 22-23 9.422655078057275E-5 0.0 0.0 2.1200973925628865E-4 0.0 24-25 1.413398261708591E-4 0.0 0.0 8.480389570251547E-4 0.0 26-27 1.413398261708591E-4 0.0 0.0 0.0015547380878794502 0.0 28-29 1.413398261708591E-4 0.0 0.0 0.004899780640589783 0.0 30-31 1.413398261708591E-4 0.0 0.0 0.01500557821180621 0.0 32-33 1.413398261708591E-4 0.0 0.0 0.03151878123610158 0.0 34-35 1.648964638660023E-4 0.0 0.0 0.0553109853081962 0.0 36-37 1.884531015611455E-4 0.0 0.0 0.09161176399641185 0.0 38-39 1.884531015611455E-4 0.0 0.0 0.14758233516007208 0.0 40-41 1.884531015611455E-4 0.0 0.0 0.21405916673576614 0.0 42-43 1.884531015611455E-4 0.0 0.0 0.2802062053837282 0.0 44-45 1.884531015611455E-4 0.0 0.0 0.36076990630111794 0.0 46-47 1.884531015611455E-4 0.0 0.0 0.46392442276814994 0.0 48-49 1.884531015611455E-4 0.0 0.0 0.5707302180779291 0.0 50-51 1.884531015611455E-4 0.0 0.0 0.6880187171620471 0.0 52-53 1.884531015611455E-4 0.0 0.0 0.8043178374629689 0.0 54-55 1.884531015611455E-4 0.0 0.0 0.9371537174258814 0.0 56-57 2.3556637695143186E-4 0.0 0.0 1.1030160034373846 0.0 58-59 2.3556637695143186E-4 0.0 0.0 1.2832713950806203 0.0 60-61 2.3556637695143186E-4 0.0 0.0 1.4937735095244198 0.0 62-63 2.3556637695143186E-4 0.0 0.0 1.7038044912143164 0.0 64-65 2.3556637695143186E-4 0.0 0.0 1.9161675800360323 0.0 66-67 2.826796523417182E-4 0.0 0.0 2.1681293768232837 0.0 68-69 2.826796523417182E-4 0.0 0.0 2.4538007221522853 0.0 70-71 3.0623629003686143E-4 0.0 0.0 2.7743358912700984 0.0 72-73 3.76906203122291E-4 0.0 0.0 3.1066493792354835 0.0 74-75 4.2401947851257736E-4 0.0 0.0 3.452908396716393 0.0 76-77 4.2401947851257736E-4 0.0 0.0 3.8165757694540137 0.0 78-79 4.2401947851257736E-4 0.0 0.0 4.245306575505619 0.0 80-81 4.711327539028637E-4 0.0 0.0 4.723883226920149 0.0 82-83 4.711327539028637E-4 0.0 0.0 5.212824798920541 0.0 84-85 4.711327539028637E-4 0.0 0.0 5.71773777127824 0.0 86-87 4.946893915980069E-4 0.0 0.0 6.249481753970707 0.0 88-89 5.182460292931501E-4 0.0 0.0 6.816183787002766 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 11890 0.0 23.44471 12-13 TATTCCG 315 0.0 22.593801 5 TTTACGC 170 3.18176E-6 22.327993 3 GTACTTT 12410 0.0 22.252052 14-15 AGTACTT 12220 0.0 21.724398 12-13 TACATGG 14085 0.0 20.919119 2 GTACATG 14335 0.0 20.823523 1 GTAAGCG 240 0.0 20.759033 94-95 CCGTTGC 190 8.3367195E-6 19.977678 9 ACATGGG 14415 0.0 19.91358 3 GGGTACG 120 0.004795683 19.769577 7 CCTATAC 505 0.0 19.730429 3 CGCCGAA 145 6.1947986E-4 19.633234 7 ACTTTTT 14370 0.0 19.414862 16-17 TACTTTT 13930 0.0 18.938368 14-15 CATGGGG 4620 0.0 18.588537 4 CATGGGA 9495 0.0 17.639585 4 ATAGTAC 490 0.0 17.429504 3 AGAGTAC 25220 0.0 17.355621 10-11 ATGGGAT 3565 0.0 17.301846 5 >>END_MODULE