##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939176_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2086846 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50279943992034 34.0 31.0 34.0 31.0 34.0 2 32.70251134966356 34.0 31.0 34.0 31.0 34.0 3 32.800419388876804 34.0 31.0 34.0 31.0 34.0 4 36.24925030404735 37.0 35.0 37.0 35.0 37.0 5 36.22162488271775 37.0 37.0 37.0 35.0 37.0 6 36.28089279228079 37.0 37.0 37.0 35.0 37.0 7 36.237828761681506 37.0 37.0 37.0 35.0 37.0 8 36.22388331482055 37.0 37.0 37.0 35.0 37.0 9 37.953892141538 39.0 38.0 39.0 35.0 39.0 10-11 37.88093347568532 39.0 38.0 39.0 35.0 39.0 12-13 37.801513384312976 39.0 38.0 39.0 35.0 39.0 14-15 39.15919957677758 40.5 38.5 41.0 36.0 41.0 16-17 39.274481681925735 41.0 39.0 41.0 36.0 41.0 18-19 39.359324070870585 41.0 39.0 41.0 36.0 41.0 20-21 39.3409226171936 41.0 39.0 41.0 36.0 41.0 22-23 39.26641041073467 41.0 39.0 41.0 36.0 41.0 24-25 39.18232179087484 41.0 39.0 41.0 36.0 41.0 26-27 39.02259318608081 40.5 39.0 41.0 35.0 41.0 28-29 38.87180917997782 40.0 38.0 41.0 35.0 41.0 30-31 38.65808833042783 40.0 38.0 41.0 35.0 41.0 32-33 38.4953465660619 40.0 38.0 41.0 35.0 41.0 34-35 38.36065239121622 40.0 38.0 41.0 34.0 41.0 36-37 38.18313258381309 40.0 38.0 41.0 34.0 41.0 38-39 38.034519557264886 40.0 38.0 41.0 34.0 41.0 40-41 37.84616042582921 40.0 37.0 41.0 33.0 41.0 42-43 37.60599296737756 40.0 37.0 41.0 33.0 41.0 44-45 37.26784750767426 40.0 37.0 41.0 32.5 41.0 46-47 36.82904129006165 40.0 36.5 41.0 31.0 41.0 48-49 36.381339111750464 40.0 36.0 41.0 30.0 41.0 50-51 36.07800958959118 39.5 36.0 41.0 29.5 41.0 52-53 35.80350514604336 39.0 35.0 41.0 28.5 41.0 54-55 35.56802035224449 39.0 35.0 40.0 28.0 41.0 56-57 35.32441253451381 39.0 35.0 40.0 27.5 41.0 58-59 34.9983422351242 38.0 35.0 40.0 26.5 41.0 60-61 34.60284132130498 38.0 34.0 40.0 26.0 41.0 62-63 34.21213472388475 37.5 34.0 40.0 25.5 41.0 64-65 33.76566526710644 37.0 34.0 39.5 24.0 41.0 66-67 33.332371674766605 36.0 33.0 39.0 22.5 40.5 68-69 32.83566276572397 36.0 33.0 39.0 21.5 40.0 70-71 32.312383616232346 35.0 32.0 38.0 20.0 40.0 72-73 31.745427070325263 35.0 31.5 37.0 18.0 39.5 74-75 31.224300691090768 35.0 31.0 37.0 14.5 39.0 76-77 29.232613714667973 32.5 28.5 35.0 9.0 37.0 78-79 30.202958915032543 34.0 30.0 36.0 7.5 37.5 80-81 30.217230212483337 34.0 30.5 35.0 7.0 37.0 82-83 29.845500338788774 34.0 30.0 35.0 2.0 37.0 84-85 29.388574672017008 34.0 29.5 35.0 2.0 36.0 86-87 28.725917724642834 34.0 29.0 35.0 2.0 36.0 88-89 28.052019650707336 33.5 28.0 35.0 2.0 35.0 90-91 27.505141730630818 33.0 26.5 35.0 2.0 35.0 92-93 27.079587808587696 33.0 26.0 35.0 2.0 35.0 94-95 26.568077376097712 32.5 25.0 34.0 2.0 35.0 96-97 25.798156404449585 32.0 24.5 34.0 2.0 35.0 98-99 24.65054560806116 32.0 11.0 34.0 2.0 35.0 100-101 18.855827646122428 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09094208319681485 1101 2 0.08483486232749016 1101 3 0.2052461321982335 1101 4 0.1259862382893857 1101 5 0.1658418099331911 1101 6 0.11821370214538263 1101 7 0.17422752312972278 1101 8 0.11471063896577505 1101 9 0.1467131997621891 1101 10-11 0.15556279354082392 1101 12-13 0.20581673594116978 1101 14-15 0.21124907042997876 1101 16-17 0.21316898783656768 1101 18-19 0.16746642606410944 1101 20-21 0.21373680360763814 1101 22-23 0.2131294177329579 1101 24-25 0.11475042659976253 1101 26-27 0.174005838644824 1101 28-29 0.19267215351339217 1101 30-31 0.18978699951265554 1101 32-33 0.29744372547828846 1101 34-35 0.38325367148247835 1101 36-37 0.442272764405665 1101 38-39 0.3785365806746128 1101 40-41 0.3930159534837472 1101 42-43 0.393773683729556 1101 44-45 0.4012228780423186 1101 46-47 0.4617376218254279 1101 48-49 0.5635551664703584 1101 50-51 0.4029526384964939 1101 52-53 0.46273799317913955 1101 54-55 0.4072315536002762 1101 56-57 0.4794586411149879 1101 58-59 0.40009088611344623 1101 60-61 0.46978186349551976 1101 62-63 0.3993607921172142 1101 64-65 0.2701080458531351 1101 66-67 0.3349830434033265 1101 68-69 0.5182711811861722 1101 70-71 0.2515822097978102 1101 72-73 0.33652054088037175 1101 74-75 0.27517040688922023 1101 76-77 0.534126256231719 1101 78-79 0.4337774833035546 1101 80-81 0.6766293136495705 1101 82-83 0.47340898901420303 1101 84-85 0.5059946240454956 1101 86-87 0.762601191509706 1101 88-89 0.602479810091733 1101 90-91 0.6495037729263409 1101 92-93 0.6784781401389459 1101 94-95 0.4625723882449826 1101 96-97 0.5272196620264218 1101 98-99 0.30487030510405333 1101 100-101 -0.7667763569442521 1102 1 0.0945748092699219 1102 2 0.09257339118468622 1102 3 0.0465100306405688 1102 4 0.06779877027527448 1102 5 0.12265058342619284 1102 6 0.1335021096376039 1102 7 0.17529170387017956 1102 8 0.14212296740291208 1102 9 0.14636298755487331 1102 10-11 0.1979786192628481 1102 12-13 0.14144105692130893 1102 14-15 0.12919880046667487 1102 16-17 0.20033158207702684 1102 18-19 0.23563397454963564 1102 20-21 0.27984467864186513 1102 22-23 0.1928325887013358 1102 24-25 0.23059810944294412 1102 26-27 0.2599437543404264 1102 28-29 0.23355362409508018 1102 30-31 0.23185938508662218 1102 32-33 0.1785524857174039 1102 34-35 0.2733450846080885 1102 36-37 0.3655937048705553 1102 38-39 0.29122345323123255 1102 40-41 0.395373597542374 1102 42-43 0.30665162510093324 1102 44-45 0.35621625593580575 1102 46-47 0.3893776849410244 1102 48-49 0.4194883127013682 1102 50-51 0.34736225520761366 1102 52-53 0.41570168816945596 1102 54-55 0.3632228427971924 1102 56-57 0.5294049967313015 1102 58-59 0.2775117763683568 1102 60-61 0.21867293879211758 1102 62-63 0.13887498393482645 1102 64-65 0.12573403082496526 1102 66-67 0.14099208506987537 1102 68-69 0.20576676725469412 1102 70-71 0.15861392915593342 1102 72-73 0.30496806564433854 1102 74-75 0.29220987541800625 1102 76-77 0.1955939162627338 1102 78-79 0.20924115168935842 1102 80-81 0.4739715706221297 1102 82-83 0.2723330055418991 1102 84-85 0.25607903292695156 1102 86-87 0.5146572370721216 1102 88-89 0.5070097372727496 1102 90-91 0.5645062060304902 1102 92-93 0.5132021642126539 1102 94-95 0.4049242663788739 1102 96-97 0.3436736376605545 1102 98-99 0.14152146375501928 1102 100-101 -0.3957037885246848 1103 1 0.17841428247038493 1103 2 0.15971667942812218 1103 3 0.1307764368185147 1103 4 0.11724614145349932 1103 5 0.14940400437959767 1103 6 0.13756879525118393 1103 7 0.09489059390718069 1103 8 0.1936061909131439 1103 9 0.1620908094689284 1103 10-11 0.17077552275969055 1103 12-13 0.14585970344651855 1103 14-15 0.055050486271603916 1103 16-17 0.23956926360196462 1103 18-19 0.26127881273858833 1103 20-21 0.2675988229659154 1103 22-23 0.211198392154067 1103 24-25 0.19427163401464753 1103 26-27 0.22129283118020737 1103 28-29 0.3016163396397715 1103 30-31 0.3170492965442975 1103 32-33 0.2891494578497529 1103 34-35 0.27534376459782095 1103 36-37 0.27343593980352665 1103 38-39 0.4427398024743283 1103 40-41 0.3797100822120001 1103 42-43 0.3164793963853185 1103 44-45 0.2803584845322291 1103 46-47 0.34888273128254355 1103 48-49 0.3637012534445816 1103 50-51 0.36756550165491575 1103 52-53 0.39067011520533157 1103 54-55 0.31793453912958825 1103 56-57 0.26381833561067936 1103 58-59 0.23012645069157855 1103 60-61 0.3154300361999631 1103 62-63 0.35135083759688257 1103 64-65 0.43723167155756926 1103 66-67 0.5051292130562857 1103 68-69 0.3330550621389605 1103 70-71 0.23057958379381915 1103 72-73 0.14520416177672146 1103 74-75 0.1647082708903227 1103 76-77 0.10546306334412847 1103 78-79 0.2105423405141913 1103 80-81 0.5539630005935159 1103 82-83 0.48905419164389485 1103 84-85 0.25647161677803254 1103 86-87 0.3784213868732067 1103 88-89 0.5990117549310234 1103 90-91 0.7937651369602001 1103 92-93 0.6315352183613037 1103 94-95 0.5671145674403419 1103 96-97 0.46061955174570457 1103 98-99 0.4991079466993469 1103 100-101 0.01585646486207537 1104 1 0.07332891161293276 1104 2 0.08962102706859554 1104 3 0.1245832252187995 1104 4 0.06281703163946872 1104 5 0.0647310320412231 1104 6 0.0012873074099175597 1104 7 0.051884387058059644 1104 8 0.10274949394352006 1104 9 0.1419855515538302 1104 10-11 0.18442104455481 1104 12-13 0.15097851586780564 1104 14-15 0.10269937831228049 1104 16-17 0.2097683239177428 1104 18-19 0.21915785863416914 1104 20-21 0.22521015847018333 1104 22-23 0.16386108773385644 1104 24-25 0.15993829514724922 1104 26-27 0.16672586385569588 1104 28-29 0.17712092777489374 1104 30-31 0.15773700688817627 1104 32-33 0.228283736401373 1104 34-35 0.2410133904080638 1104 36-37 0.29812962930927966 1104 38-39 0.2449104208586519 1104 40-41 0.3679154086704486 1104 42-43 0.3718551277712905 1104 44-45 0.359096765161496 1104 46-47 0.4123004472474463 1104 48-49 0.4079120088207233 1104 50-51 0.29830084843918314 1104 52-53 0.21221134438440004 1104 54-55 0.23471277649827726 1104 56-57 0.3443078513246576 1104 58-59 0.33496427096569903 1104 60-61 0.3521235549402846 1104 62-63 0.3291682300561618 1104 64-65 0.2584844376018154 1104 66-67 0.4277194044795962 1104 68-69 0.3868761165680752 1104 70-71 0.3508123821411857 1104 72-73 0.08043040225714293 1104 74-75 0.08984245220052145 1104 76-77 0.12464097009197062 1104 78-79 0.3795603918434338 1104 80-81 0.5505266427355799 1104 82-83 0.45793989976308325 1104 84-85 0.09603008140705782 1104 86-87 0.015082353452989139 1104 88-89 0.2998502843560402 1104 90-91 0.4570622498385255 1104 92-93 0.3812953759398532 1104 94-95 0.5319534905745513 1104 96-97 0.1445517104342251 1104 98-99 0.012616796855049017 1104 100-101 -0.5908639257164161 1105 1 0.005286885947313635 1105 2 0.07811985000120103 1105 3 0.04090641542813245 1105 4 0.04198254237574872 1105 5 -0.04099696631008953 1105 6 -0.0045901032003357045 1105 7 -0.01614178584798509 1105 8 -0.030211563752594373 1105 9 -0.016129872388589206 1105 10-11 -0.011054379704667383 1105 12-13 0.052039461801967946 1105 14-15 0.08145469889471002 1105 16-17 0.12237998679295004 1105 18-19 0.11416899519776535 1105 20-21 0.07751891474192973 1105 22-23 0.13430658990964162 1105 24-25 0.1190282674841896 1105 26-27 0.1845453564088615 1105 28-29 0.18536337195336472 1105 30-31 0.20560045121725068 1105 32-33 0.1758200194048385 1105 34-35 0.17754686747309023 1105 36-37 0.26539269000156906 1105 38-39 0.16009023078406415 1105 40-41 0.2118806594214675 1105 42-43 0.31018293906004857 1105 44-45 0.44659012681842114 1105 46-47 0.4155254930222938 1105 48-49 0.4396462610856915 1105 50-51 0.40409721181614344 1105 52-53 0.5503645771583976 1105 54-55 0.44724577005413124 1105 56-57 0.3658844478957093 1105 58-59 0.41034513351435464 1105 60-61 0.19502171681281055 1105 62-63 0.2335850070316141 1105 64-65 0.27635545162358 1105 66-67 0.35499189756382776 1105 68-69 0.22663939523581433 1105 70-71 0.22182937629944632 1105 72-73 0.14871517330366402 1105 74-75 0.28663860949160025 1105 76-77 0.01272445072386219 1105 78-79 0.1401460720457166 1105 80-81 0.3760046034365878 1105 82-83 0.4301262330990596 1105 84-85 0.09511557533940973 1105 86-87 0.17807250096291938 1105 88-89 -0.033273660444653075 1105 90-91 -0.0634740093858035 1105 92-93 -0.08166960531426426 1105 94-95 -0.04546346078125296 1105 96-97 -0.42425917407049596 1105 98-99 -0.5793292086458592 1105 100-101 -1.1159717244814864 1106 1 0.038489288410943345 1106 2 0.0673803947591125 1106 3 0.02079929216403542 1106 4 0.007211600150299091 1106 5 0.10060594760053476 1106 6 0.09142705537509244 1106 7 0.04489753890167236 1106 8 0.07336193373483724 1106 9 0.013772129500011943 1106 10-11 0.11185183619589623 1106 12-13 0.13769244731626884 1106 14-15 0.11856762663281728 1106 16-17 0.09626848564779067 1106 18-19 0.13471661945192892 1106 20-21 0.21807010684859307 1106 22-23 0.16519756105059713 1106 24-25 0.13674928662946684 1106 26-27 0.12116007273171192 1106 28-29 0.30134858427347666 1106 30-31 0.16185507202467875 1106 32-33 0.1300654624460762 1106 34-35 0.09812214121397034 1106 36-37 0.03766916075421989 1106 38-39 0.14964151989685348 1106 40-41 0.17438505072993848 1106 42-43 0.062185266110994064 1106 44-45 0.1464696090358899 1106 46-47 0.06603118541860198 1106 48-49 0.023557520756845918 1106 50-51 0.013898831544445045 1106 52-53 0.12666935068197205 1106 54-55 -0.01006262777183764 1106 56-57 -0.08481780522232896 1106 58-59 -0.05959461307494962 1106 60-61 0.10241758457098626 1106 62-63 0.11823951450081438 1106 64-65 0.04558965675153104 1106 66-67 0.04524516576604043 1106 68-69 -0.12204296559739447 1106 70-71 -0.17404756183640302 1106 72-73 -0.055757576469304126 1106 74-75 -0.01673544997370513 1106 76-77 1.528401974368876E-4 1106 78-79 0.16117146518086045 1106 80-81 0.271465018085717 1106 82-83 0.24972566355799586 1106 84-85 -0.10596497427428986 1106 86-87 0.0751920218293769 1106 88-89 -0.026029714147679073 1106 90-91 0.20448034512062563 1106 92-93 0.2196184113598285 1106 94-95 0.21239581675875385 1106 96-97 -0.18213968882775688 1106 98-99 -0.22782462716153162 1106 100-101 -1.1072088376010463 1107 1 0.04912924396236917 1107 2 0.04562645999547499 1107 3 0.056294141911394036 1107 4 0.01654989250039307 1107 5 -0.06496681831047368 1107 6 0.00897763057801626 1107 7 0.07465373806611098 1107 8 0.11812634633636065 1107 9 0.06292736005075028 1107 10-11 0.1094119390907693 1107 12-13 0.050862041807000935 1107 14-15 0.042939079653059764 1107 16-17 0.012168372480218181 1107 18-19 0.05010733089664399 1107 20-21 -0.00525956965584129 1107 22-23 0.08863762447059997 1107 24-25 0.0478708995375996 1107 26-27 0.10124714408394198 1107 28-29 0.040754273501782734 1107 30-31 0.04545904742266771 1107 32-33 0.07919409310792247 1107 34-35 -0.06991544280260342 1107 36-37 0.031038863008596707 1107 38-39 -0.016052178447814924 1107 40-41 0.1096161575948642 1107 42-43 0.10893011126763952 1107 44-45 0.04402304884222019 1107 46-47 0.09885148716732317 1107 48-49 -0.003464320007928734 1107 50-51 -0.12275193290068387 1107 52-53 -0.029703762339231332 1107 54-55 -0.1134849107138578 1107 56-57 -0.009442271978500116 1107 58-59 -0.09043067853690445 1107 60-61 -0.10476302132936155 1107 62-63 -0.19664860594684086 1107 64-65 -0.17925600355792426 1107 66-67 -0.2590099507776742 1107 68-69 -0.2129890087458719 1107 70-71 -0.3120625543102591 1107 72-73 -0.4271736086997677 1107 74-75 -0.4104762390407828 1107 76-77 -0.3751579509105376 1107 78-79 -0.27835680913691974 1107 80-81 -0.049435228133766884 1107 82-83 -0.22220791226978776 1107 84-85 -0.23131419618819038 1107 86-87 -0.314436802166977 1107 88-89 -0.5763780048031428 1107 90-91 -0.2456358129740046 1107 92-93 -0.3253237273236991 1107 94-95 -0.27236249937908497 1107 96-97 -0.5529173499577631 1107 98-99 -0.6118093692134394 1107 100-101 -1.0644855860977813 1108 1 -0.010039172501421945 1108 2 -0.0039850038508646435 1108 3 0.01741679342550384 1108 4 0.03201439337414769 1108 5 0.016879468518020246 1108 6 -0.00900957602129182 1108 7 0.046209968697077386 1108 8 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-1.008411490790646 2316 96-97 -1.111554054728419 2316 98-99 -0.8885383396642013 2316 100-101 0.38242254173691137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 10.0 5 45.0 6 93.0 7 230.0 8 550.0 9 1045.0 10 1777.0 11 3098.0 12 4802.0 13 6938.0 14 8575.0 15 11970.0 16 17075.0 17 26185.0 18 25618.0 19 15349.0 20 12540.0 21 12783.0 22 13703.0 23 14449.0 24 15875.0 25 18385.0 26 21372.0 27 25416.0 28 30810.0 29 37117.0 30 45179.0 31 55741.0 32 70412.0 33 92513.0 34 130469.0 35 199577.0 36 325589.0 37 504992.0 38 322466.0 39 14091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58835288988048 19.883863851396548 15.69503144030577 20.832751818417194 2 15.09723285762342 21.889013372333178 39.760049375948206 23.253704394095205 3 19.63273763373052 26.46740583636742 30.625403120306917 23.27445340959515 4 12.48760090586464 17.866627436811342 38.16889219424912 31.476879463074898 5 12.780483083083274 38.9173422475832 33.60430046107859 14.697874208254946 6 29.34030589703313 37.598605742829136 17.52625732804433 15.534831032093408 7 26.84615922784911 34.71061113278124 20.962591393902567 17.480638245467084 8 26.021182205107614 33.664918254629235 19.88867410436611 20.425225435897044 9 26.66157445254705 17.612080623102997 21.192411898146773 34.53393302620318 10-11 24.6955213753195 27.248033635447943 26.624437069146456 21.4320079200861 12-13 26.812831421197348 25.083738809667793 27.265524145049515 20.837905624085344 14-15 23.306763553558696 25.949166008571844 27.547282196171036 23.19678824169842 16-17 20.726513235796578 29.329313787645507 27.951224911982585 21.992948064575334 18-19 20.733010485680303 27.842519285083807 30.90728784011853 20.51718238911736 20-21 22.55101718095154 26.10171042808142 30.398673404745725 20.948598986221313 22-23 21.72836423962286 26.26216788397419 30.61723289595878 21.392234980444172 24-25 21.890964590086952 26.532900143518084 30.75496742690521 20.821167839489757 26-27 21.51996152918149 26.185914016187166 31.50979513139045 20.784329323240893 28-29 20.78115030556886 27.304273363792387 31.07738450211096 20.837191828527796 30-31 21.235658980106823 26.58511936194621 30.75068788017899 21.42853377776798 32-33 21.237461038682547 26.66330593232705 30.802591758709497 21.296641270280908 34-35 22.003387318928443 26.450729078704843 30.467299528516644 21.07858407385007 36-37 21.911634586164883 26.092093519959125 31.05251654353084 20.943755350345153 38-39 21.453432808065084 26.738475268233813 30.83526566482588 20.972826258875223 40-41 21.413946333345713 26.86330302598269 30.642348901543244 21.08040173912835 42-43 21.118075972904133 27.068815550172165 29.76400140894681 22.04910706797689 44-45 21.601016305307546 26.6504358760374 29.763182365895307 21.98536545275975 46-47 22.186070583950528 27.231248816931714 29.207494003790615 21.37518659532715 48-49 21.972072682523358 27.133292824234918 29.084438468181027 21.81019602506069 50-51 21.47882886314965 27.561700131347735 29.389331267038425 21.570139738464185 52-53 21.863712032416384 28.07368152705087 28.29219309905954 21.77041334147321 54-55 21.338565471529762 27.414480991889196 28.70825159115718 22.53870194542386 56-57 21.94371840176956 27.530859376029515 28.269126775317098 22.256295446883826 58-59 22.470604161600143 27.07944034396875 28.41170825910747 22.038247235323645 60-61 21.853323538417968 27.408583585274464 29.25346835365395 21.48462452265362 62-63 21.54806467356888 27.52932274656373 29.076941765659065 21.845670814208326 64-65 21.35006047571675 27.933359785469342 28.704329332926964 22.01225040588694 66-67 21.55014814498897 27.460972672604605 28.235241687420064 22.75363749498636 68-69 21.330933760726257 27.481184352492775 28.671761804960365 22.516120081820603 70-71 21.903955948802125 27.505499331646416 28.27271030496043 22.317834414591026 72-73 21.697767827621202 27.028467840942742 28.75315188566861 22.52061244576744 74-75 21.16914712441646 28.421215556873864 27.941520361349138 22.468116957360536 76-77 21.403171918622483 27.342011433565332 28.268947080248648 22.985869567563537 78-79 21.41477695766376 27.473611890926353 28.47294162924153 22.63866952216836 80-81 21.614291262656987 27.623060975162705 28.07402958010946 22.688618182070847 82-83 21.357613355273937 27.336612284758914 28.530064029640904 22.775710330326245 84-85 21.14506772421156 27.41884163948849 28.306185506740793 23.129905129559152 86-87 21.075217286601482 27.59112893089907 28.23831152072318 23.095342261776267 88-89 21.08962962053965 27.29377251724683 27.863650002249766 23.75294785996375 90-91 21.117566730233595 26.882457332956346 28.289239543040022 23.71073639377004 92-93 21.35591222351817 27.265907498684616 28.16101427704776 23.217166000749458 94-95 20.882044214581292 26.957769513842784 28.35662726349411 23.803559008081816 96-97 20.336023836929034 26.859073453431638 28.735134264818775 24.069768444820557 98-99 20.599354671610655 27.113663715720076 27.86292893409164 24.42405267857763 100-101 20.847409626603262 25.74028124793348 28.75768745309899 24.65462167236427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1472.0 1 1019.0 2 680.5 3 792.5 4 672.0 5 663.5 6 1221.0 7 2674.5 8 4271.0 9 4911.5 10 4406.5 11 3780.5 12 3824.5 13 3986.5 14 4146.0 15 4326.5 16 4480.5 17 4583.0 18 4824.5 19 5113.0 20 5410.5 21 6098.0 22 7036.5 23 8214.0 24 9531.5 25 11189.0 26 14100.0 27 17321.0 28 19629.5 29 22658.0 30 26483.5 31 30194.0 32 34595.5 33 38981.5 34 43695.5 35 47612.5 36 52705.0 37 58289.5 38 59276.5 39 58758.5 40 59115.5 41 60584.5 42 61927.0 43 63576.5 44 66805.0 45 69687.5 46 71108.5 47 81457.5 48 107954.0 49 105655.0 50 79248.0 51 67626.0 52 62381.0 53 60648.0 54 59227.0 55 55388.5 56 50076.0 57 45685.0 58 42562.5 59 38439.0 60 32437.0 61 26445.5 62 21268.0 63 16507.0 64 12318.0 65 9133.5 66 6717.5 67 5251.5 68 4027.5 69 2840.5 70 2060.0 71 1469.0 72 1134.0 73 989.5 74 757.5 75 531.5 76 368.5 77 215.0 78 130.5 79 87.0 80 55.0 81 26.0 82 10.0 83 7.5 84 5.0 85 3.0 86 1.5 87 1.5 88 1.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4283976872275194 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 6.7086886143012E-4 16-17 0.0020605257886782257 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.791920438786571E-5 26-27 0.003330384704956667 28-29 2.3959602193932854E-5 30-31 0.0 32-33 2.3959602193932854E-5 34-35 0.011500609053087769 36-37 0.025493016734344558 38-39 0.020126065842903596 40-41 0.008074385939355371 42-43 0.008673375994203694 44-45 0.003426223113732398 46-47 0.005319031687053093 48-49 0.0034501827159263312 50-51 9.104648833694485E-4 52-53 0.0 54-55 0.0 56-57 2.3959602193932854E-5 58-59 0.0013656973250541727 60-61 0.0023480410150054196 62-63 0.001317778120666307 64-65 0.002779313854496211 66-67 0.0018688489711267625 68-69 0.004097091975162518 70-71 7.187880658179856E-5 72-73 0.0 74-75 0.0 76-77 0.0967728332612948 78-79 0.016651923524783334 80-81 2.156364197453957E-4 82-83 0.0 84-85 0.0 86-87 0.1088724323692309 88-89 0.42434851445674476 90-91 0.43048217261839156 92-93 0.0 94-95 2.3959602193932854E-5 96-97 0.0 98-99 7.187880658179856E-5 100-101 1.9167681755146283E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2086846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.46438302018121 #Duplication Level Percentage of deduplicated Percentage of total 1 68.23277513587217 28.974626978995726 2 15.679030860950565 13.316007437292926 3 6.284836581260341 8.006451234176561 4 3.1190937965841403 5.298015746160805 5 1.7732259512188722 3.764947298694167 6 1.1235626557459575 2.8626836976461005 7 0.743398997292701 2.2097585830499136 8 0.5219832217188022 1.7732556365740306 9 0.38041079201959815 1.4538518619597638 >10 1.9085286597208329 14.900766280197095 >50 0.14923483125388057 4.355215824663106 >100 0.07567521924622035 5.775495907674481 >500 0.003952265740483899 1.060640974014239 >1k 0.0036135001055852783 3.288854943022497 >5k 3.387656348986199E-4 1.010052058259231 >10k+ 3.387656348986199E-4 1.94937553761928 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14583 0.6988057575882456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14498 0.694732625215277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11572 0.5545210331763819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7837 0.3755428047877036 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7734 0.37060712673575336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5493 0.26322018970254635 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4262 0.20423164910108363 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4159 0.1992959710491335 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3828 0.1834347143967499 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3789 0.18156586542562317 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3774 0.18084707735980518 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3213 0.15396440369821252 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3212 0.15391648449382464 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3136 0.15027462496034685 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3113 0.14917248325942595 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2760 0.13225700411050936 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2685 0.12866306378141942 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2439 0.11687493950200446 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2148 0.10293045102513555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.791920438786571E-5 0.0 0.0 0.0 0.0 7 4.791920438786571E-5 0.0 0.0 0.0 0.0 8 4.791920438786571E-5 0.0 0.0 0.0 0.0 9 4.791920438786571E-5 0.0 0.0 0.0 0.0 10-11 4.791920438786571E-5 0.0 0.0 0.0 0.0 12-13 4.791920438786571E-5 0.0 0.0 2.3959602193932854E-5 0.0 14-15 4.791920438786571E-5 0.0 0.0 7.187880658179856E-5 0.0 16-17 4.791920438786571E-5 0.0 0.0 9.583840877573142E-5 0.0 18-19 4.791920438786571E-5 0.0 0.0 9.583840877573142E-5 0.0 20-21 4.791920438786571E-5 0.0 0.0 2.1563641974539567E-4 0.0 22-23 4.791920438786571E-5 0.0 0.0 5.750304526543885E-4 0.0 24-25 9.583840877573142E-5 0.0 0.0 0.0011021417009209112 0.0 26-27 9.583840877573142E-5 0.0 0.0 0.002300121810617554 0.0 28-29 9.583840877573142E-5 0.0 0.0 0.005965940946289281 0.0 30-31 9.583840877573142E-5 0.0 0.0 0.01679568113794693 0.0 32-33 9.583840877573142E-5 0.0 0.0 0.03359136227589386 0.0 34-35 1.1979801096966427E-4 0.0 0.0 0.05901250020365662 0.0 36-37 2.3959602193932854E-4 0.0 0.0 0.09873752064119728 0.0 38-39 2.3959602193932854E-4 0.0 0.0 0.15739062681194493 0.0 40-41 2.3959602193932854E-4 0.0 0.0 0.22306389642551488 0.0 42-43 2.3959602193932854E-4 0.0 0.0 0.29225922756159295 0.0 44-45 2.3959602193932854E-4 0.0 0.0 0.37324268297708596 0.0 46-47 2.3959602193932854E-4 0.0 0.0 0.47310630492139816 0.0 48-49 2.3959602193932854E-4 0.0 0.0 0.5833444346156832 0.0 50-51 2.3959602193932854E-4 0.0 0.0 0.7027351323480506 0.0 52-53 2.3959602193932854E-4 0.0 0.0 0.8233717293945025 0.0 54-55 2.3959602193932854E-4 0.0 0.0 0.9554370566874604 0.0 56-57 2.3959602193932854E-4 0.0 0.0 1.119656170124676 0.0 58-59 2.3959602193932854E-4 0.0 0.0 1.2972926607904944 0.0 60-61 2.635556241332614E-4 0.0 0.0 1.5049744926075044 0.0 62-63 2.8751522632719425E-4 0.0 0.0 1.7171367700347797 0.0 64-65 2.8751522632719425E-4 0.0 0.0 1.9349055943754354 0.0 66-67 2.8751522632719425E-4 0.0 0.0 2.1874158418972938 0.0 68-69 2.8751522632719425E-4 0.0 0.0 2.481328282010268 0.0 70-71 3.3543443071505993E-4 0.0 0.0 2.8069871950302034 0.0 72-73 3.3543443071505993E-4 0.0 0.0 3.1412955244421488 0.0 74-75 3.3543443071505993E-4 0.0 0.0 3.4949392528245973 0.0 76-77 3.3543443071505993E-4 0.0 0.0 3.8596762770228374 0.0 78-79 3.3543443071505993E-4 0.0 0.0 4.29473473366027 0.0 80-81 4.3127283949079135E-4 0.0 0.0 4.779102051612817 0.0 82-83 4.3127283949079135E-4 0.0 0.0 5.264212117233376 0.0 84-85 4.3127283949079135E-4 0.0 0.0 5.762476004458403 0.0 86-87 4.3127283949079135E-4 0.0 0.0 6.2814170283767945 0.0 88-89 4.3127283949079135E-4 0.0 0.0 6.840490385970023 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 315 0.0 30.12717 94-95 GAGTACT 11940 0.0 23.109722 12-13 AGTACTT 12160 0.0 22.672108 12-13 GTACTTT 12590 0.0 21.784693 14-15 GTACATG 14640 0.0 21.061012 1 TACATGG 14585 0.0 20.91912 2 GTAAGGT 710 0.0 20.717735 4 ACATGGG 14490 0.0 20.073866 3 ACTACGC 120 0.0047937203 19.770956 8 ACTTTTT 14240 0.0 19.460186 16-17 TACTTTT 14195 0.0 19.23797 14-15 ATGGGAT 3795 0.0 19.13016 5 TAAGGTG 930 0.0 18.878075 5 CATGGGG 4240 0.0 18.577238 4 ATCGCGT 65 0.007610093 18.250988 24-25 CATGGGA 9980 0.0 18.067245 4 AGAGTAC 25535 0.0 17.718794 10-11 CGTATAG 270 4.7134745E-7 17.650307 1 TATAACG 190 1.8815993E-4 17.481688 2 TACCTGG 1365 0.0 17.03344 2 >>END_MODULE