##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939175_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533345 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20652485726875 33.0 31.0 34.0 31.0 34.0 2 32.284504401466215 34.0 31.0 34.0 31.0 34.0 3 32.29643476548951 34.0 31.0 34.0 30.0 34.0 4 35.748687997450055 37.0 35.0 37.0 35.0 37.0 5 35.819709568853185 37.0 35.0 37.0 35.0 37.0 6 35.94238438534157 37.0 37.0 37.0 35.0 37.0 7 35.90039092894843 37.0 37.0 37.0 35.0 37.0 8 35.911646307737016 37.0 37.0 37.0 35.0 37.0 9 37.570979384825954 39.0 38.0 39.0 35.0 39.0 10-11 37.519801441843455 39.0 38.0 39.0 35.0 39.0 12-13 37.46404953641639 39.0 38.0 39.0 35.0 39.0 14-15 38.68889461793023 40.0 38.0 41.0 35.0 41.0 16-17 38.64625617564616 40.0 38.0 41.0 34.5 41.0 18-19 38.72259325577252 40.0 38.5 41.0 35.0 41.0 20-21 38.69628476877068 40.0 38.5 41.0 35.0 41.0 22-23 38.61956050961385 40.0 38.0 41.0 35.0 41.0 24-25 38.61304127722206 40.5 38.0 41.0 35.0 41.0 26-27 38.49308983865978 40.0 38.0 41.0 34.5 41.0 28-29 38.37596771320627 40.0 38.0 41.0 34.0 41.0 30-31 38.169672538413224 40.0 38.0 41.0 34.0 41.0 32-33 37.86894036692948 40.0 37.0 41.0 34.0 41.0 34-35 37.543982787876516 40.0 36.5 41.0 33.0 41.0 36-37 37.235831403688046 40.0 36.0 41.0 33.0 41.0 38-39 36.96971285003141 40.0 35.5 41.0 32.5 41.0 40-41 36.83705387694644 40.0 35.0 41.0 31.5 41.0 42-43 36.587244654023195 40.0 35.0 41.0 31.0 41.0 44-45 36.20298024730709 40.0 35.0 41.0 30.0 41.0 46-47 35.64223626358174 39.5 35.0 41.0 28.0 41.0 48-49 35.34032661785523 40.0 35.0 41.0 24.0 41.0 50-51 34.27609896033525 38.5 34.0 40.0 22.5 40.5 52-53 34.4293571703119 38.5 35.0 40.0 22.5 40.5 54-55 34.95163262053643 39.5 35.0 41.0 22.0 41.0 56-57 34.85285415631533 40.0 35.0 41.0 20.5 41.0 58-59 34.715340914417496 39.0 35.0 41.0 18.5 41.0 60-61 34.401239347889266 39.0 35.0 41.0 14.0 41.0 62-63 34.07938576343642 39.0 35.0 41.0 10.0 41.0 64-65 33.71482811313503 38.0 34.5 40.0 6.0 41.0 66-67 33.27892921092351 37.5 34.0 40.0 2.0 41.0 68-69 32.84939016959004 37.0 34.0 39.5 2.0 41.0 70-71 32.40279837628552 36.5 33.5 39.0 2.0 41.0 72-73 31.90690359898377 36.0 33.0 39.0 2.0 40.0 74-75 31.43626358173415 35.0 32.5 37.5 2.0 40.0 76-77 30.967647582709127 35.0 32.0 37.0 2.0 39.0 78-79 30.516541825647565 35.0 31.5 36.5 2.0 39.0 80-81 30.063458924336032 35.0 31.0 36.0 2.0 38.5 82-83 29.61737993231398 35.0 31.0 36.0 2.0 37.0 84-85 29.205323946038682 35.0 30.0 35.0 2.0 37.0 86-87 28.86613355332852 34.0 30.0 35.0 2.0 36.5 88-89 28.42231857428119 34.0 29.0 35.0 2.0 36.0 90-91 28.020980791045197 34.0 29.0 35.0 2.0 36.0 92-93 27.640699734693307 34.0 29.0 35.0 2.0 35.5 94-95 27.21654088816807 34.0 27.0 35.0 2.0 35.0 96-97 26.676721446718354 34.0 25.5 35.0 2.0 35.0 98-99 26.09655382538507 34.0 24.5 35.0 2.0 35.0 100-101 23.822751689806786 31.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13568935239563729 1101 2 0.19475627991047162 1101 3 0.36665774708570353 1101 4 0.1338740997474872 1101 5 0.06517791690649233 1101 6 0.2746861718256426 1101 7 0.16089742644459903 1101 8 0.2684701883981475 1101 9 0.4451206466660764 1101 10-11 0.4207584102371271 1101 12-13 0.25241139462725926 1101 14-15 0.3212378663626083 1101 16-17 0.41654086277212343 1101 18-19 0.46279133658195803 1101 20-21 0.5032242310221662 1101 22-23 0.5230560618912392 1101 24-25 0.6367797435711822 1101 26-27 0.4209225744116054 1101 28-29 0.4718129765910035 1101 30-31 0.6469596447049923 1101 32-33 0.323896165953208 1101 34-35 0.5391624765446039 1101 36-37 0.5917081030082443 1101 38-39 0.3555566427213037 1101 40-41 0.395336928788673 1101 42-43 0.3248497028036823 1101 44-45 0.3019047835178199 1101 46-47 0.2873052992920009 1101 48-49 0.598850006279072 1101 50-51 0.21730721414896692 1101 52-53 0.2986352018314804 1101 54-55 0.2176183293655356 1101 56-57 0.20026501900584037 1101 58-59 -0.15055861481788924 1101 60-61 0.0248757395201622 1101 62-63 -0.020680555145894175 1101 64-65 0.15996276595751624 1101 66-67 0.04204651778930213 1101 68-69 0.07576065068548132 1101 70-71 -0.2563541653916346 1101 72-73 -0.372927164477975 1101 74-75 -0.3584041149180166 1101 76-77 -0.39117064638991295 1101 78-79 -0.2446005408367995 1101 80-81 -0.4306315005736643 1101 82-83 -0.3613029418218012 1101 84-85 -0.35237953268278943 1101 86-87 -0.40380114561055436 1101 88-89 -0.3737984973146844 1101 90-91 -0.24702756291729955 1101 92-93 -0.48848524826868456 1101 94-95 -0.6972081270288975 1101 96-97 -0.9050165156238776 1101 98-99 -0.5625966369142787 1101 100-101 -0.5178346554069044 1102 1 0.041200855087758725 1102 2 0.19039015166769246 1102 3 0.2706225047949573 1102 4 0.24850943993349262 1102 5 -0.006833830523753193 1102 6 0.1701536216543289 1102 7 0.08160227225958039 1102 8 0.04342858291601459 1102 9 0.20761697559412795 1102 10-11 0.26820334415783975 1102 12-13 0.23566641175892755 1102 14-15 0.5083450616636327 1102 16-17 0.5242158505352208 1102 18-19 0.43587014225987986 1102 20-21 0.4165673538807013 1102 22-23 0.27038352150944434 1102 24-25 0.3683705414174909 1102 26-27 0.5384849826348059 1102 28-29 0.575454679968395 1102 30-31 0.477052645194469 1102 32-33 0.6573763421646674 1102 34-35 0.8260004599024029 1102 36-37 0.6264413384463197 1102 38-39 0.7136917381691745 1102 40-41 0.8292380545840672 1102 42-43 0.7471796098031973 1102 44-45 0.8172107061805747 1102 46-47 1.0586905171089356 1102 48-49 0.9913952828331603 1102 50-51 1.1325152415596378 1102 52-53 1.187029719697371 1102 54-55 1.1742654169817897 1102 56-57 1.2405929681003016 1102 58-59 1.3953444688825556 1102 60-61 1.3961630620850158 1102 62-63 1.4450365275755956 1102 64-65 1.4185383900397426 1102 66-67 1.5824234145098117 1102 68-69 1.6925447916546403 1102 70-71 1.6582371219309309 1102 72-73 1.4919039662121847 1102 74-75 1.2023056256595694 1102 76-77 1.3062547084803775 1102 78-79 1.3408718037544887 1102 80-81 1.1791727089221773 1102 82-83 1.1784767938610194 1102 84-85 1.5826669675619485 1102 86-87 1.2435214192450488 1102 88-89 1.3340233333265274 1102 90-91 1.362810909916771 1102 92-93 1.2836978158527081 1102 94-95 1.1084893766421722 1102 96-97 1.084170953783854 1102 98-99 0.8145546112501805 1102 100-101 0.7293949089592253 1103 1 0.18213119974912217 1103 2 0.11544679182194528 1103 3 0.19990666965395576 1103 4 0.14412284075296355 1103 5 0.1404691340460218 1103 6 0.11485836978641117 1103 7 0.09678665403832554 1103 8 0.18760308279550486 1103 9 0.3730688917184892 1103 10-11 0.35842026389752846 1103 12-13 0.25091827967891334 1103 14-15 0.3877304557344985 1103 16-17 0.2651437849772549 1103 18-19 0.3791697325225911 1103 20-21 0.32262182483756163 1103 22-23 0.4050617572288999 1103 24-25 0.318715202781668 1103 26-27 0.3713989378047273 1103 28-29 0.3137482446827846 1103 30-31 0.3189808206584388 1103 32-33 0.2471811143989342 1103 34-35 0.18122340519042268 1103 36-37 0.3230477496297368 1103 38-39 0.3405753245755392 1103 40-41 0.43829324860671903 1103 42-43 0.18149872284248403 1103 44-45 0.31453295250499025 1103 46-47 0.3139520259062323 1103 48-49 0.2935259167208386 1103 50-51 0.1780418744832346 1103 52-53 0.32632352543369336 1103 54-55 0.434029215487449 1103 56-57 0.3507351710407747 1103 58-59 0.2753661608565281 1103 60-61 0.42587624598475315 1103 62-63 0.3155402634155493 1103 64-65 0.2071356423897086 1103 66-67 0.19839834822877833 1103 68-69 0.07230617994098054 1103 70-71 0.5237637548545351 1103 72-73 0.320826598171692 1103 74-75 0.1547544084988033 1103 76-77 0.1555268222427273 1103 78-79 0.17284095294706603 1103 80-81 0.18955111335697694 1103 82-83 0.28088460297164985 1103 84-85 0.20488918978416848 1103 86-87 0.3412823254875157 1103 88-89 0.20557310435568965 1103 90-91 0.1228253712327465 1103 92-93 0.0197161335196121 1103 94-95 -0.0778736023889195 1103 96-97 -0.2279235268138784 1103 98-99 -0.46120099250975954 1103 100-101 -0.7555098873772437 1104 1 0.3866979311111507 1104 2 0.36594570722324704 1104 3 0.2516751363739047 1104 4 0.23318780250659188 1104 5 0.36228897649378666 1104 6 0.3756506976777203 1104 7 0.435286482785898 1104 8 0.531966594611923 1104 9 0.5005994317344786 1104 10-11 0.5603086073157328 1104 12-13 0.42701144099869737 1104 14-15 0.6737836581548606 1104 16-17 0.7020521078451623 1104 18-19 0.6730046451838589 1104 20-21 0.5889650146994185 1104 22-23 0.7384536969480422 1104 24-25 0.6012360384935107 1104 26-27 0.6409411229856872 1104 28-29 0.6705423992666368 1104 30-31 0.7530760370073395 1104 32-33 0.6384289737436077 1104 34-35 0.8485735593176145 1104 36-37 0.9568506951714681 1104 38-39 0.8172005100989992 1104 40-41 0.8246181715431291 1104 42-43 0.928524639042962 1104 44-45 1.1765674313267738 1104 46-47 0.9954156633077673 1104 48-49 1.0435590255232157 1104 50-51 1.1871936041327373 1104 52-53 1.123462468235381 1104 54-55 1.100347288326816 1104 56-57 1.0173181142991368 1104 58-59 1.3421865741457069 1104 60-61 1.2545256351844358 1104 62-63 1.3719371123709152 1104 64-65 1.3678366356537808 1104 66-67 1.6141193209425566 1104 68-69 1.219386896917797 1104 70-71 1.5276523266092923 1104 72-73 1.3859390539314873 1104 74-75 1.1325395437882264 1104 76-77 1.4942664043868028 1104 78-79 1.3371291136960117 1104 80-81 1.1392896679864997 1104 82-83 1.187424162282074 1104 84-85 1.1743628739946956 1104 86-87 1.148726097607625 1104 88-89 1.04887713449612 1104 90-91 1.0898284537764162 1104 92-93 0.6238147749170331 1104 94-95 0.7081384994491913 1104 96-97 0.3432352812692372 1104 98-99 0.5273616287940399 1104 100-101 0.55647093235104 1105 1 0.3990465215281276 1105 2 0.48431637932100813 1105 3 0.5056530546931199 1105 4 0.4451376363580195 1105 5 0.4923650832576456 1105 6 0.5009931781150669 1105 7 0.5154108262310686 1105 8 0.4848361411871167 1105 9 0.4794567583504019 1105 10-11 0.47856994293584165 1105 12-13 0.6201832707768062 1105 14-15 0.685120350190445 1105 16-17 0.6289703198721739 1105 18-19 0.7241140888541295 1105 20-21 0.7857277974199661 1105 22-23 0.7595620686920697 1105 24-25 0.7146887129495099 1105 26-27 0.8188787368778421 1105 28-29 0.712834177592292 1105 30-31 0.630132527520793 1105 32-33 0.7376207413500069 1105 34-35 0.5048689821925194 1105 36-37 0.4540765500326529 1105 38-39 0.4668349832472458 1105 40-41 0.6069185252787932 1105 42-43 0.6131424973343158 1105 44-45 0.6387970379298409 1105 46-47 0.8562646288989342 1105 48-49 0.6134228908892752 1105 50-51 0.5988175724037106 1105 52-53 0.5515533675815121 1105 54-55 0.48305926174304403 1105 56-57 0.42533612266016263 1105 58-59 0.4389086236687021 1105 60-61 0.6160499143140328 1105 62-63 0.5734185144504522 1105 64-65 0.7277916147512187 1105 66-67 0.6888004228827427 1105 68-69 0.7875957081515921 1105 70-71 0.8233581900458944 1105 72-73 0.8903425269725389 1105 74-75 0.797238868474679 1105 76-77 0.8974586007636951 1105 78-79 0.9946486763500992 1105 80-81 0.8281566419386941 1105 82-83 0.891328117689941 1105 84-85 0.8973535482363069 1105 86-87 1.0682351553844462 1105 88-89 0.9290357795813371 1105 90-91 1.0315821251238297 1105 92-93 0.77915832728063 1105 94-95 0.8094805511160352 1105 96-97 0.686393176006078 1105 98-99 0.8403726696344336 1105 100-101 0.8126784572733214 1106 1 0.08829059011336682 1106 2 0.19897175932745625 1106 3 0.3920653905665006 1106 4 0.29551456128731246 1106 5 0.15447545471338486 1106 6 0.3211712123913557 1106 7 0.2298988654459535 1106 8 0.10368687728252723 1106 9 0.29203915328283614 1106 10-11 0.2507885144985238 1106 12-13 0.35290533404794644 1106 14-15 0.291718475157289 1106 16-17 0.3712410119691967 1106 18-19 0.3756942348895862 1106 20-21 0.5877942521216326 1106 22-23 0.43594130374057016 1106 24-25 0.45505947483988507 1106 26-27 0.4180975410850394 1106 28-29 0.25851413220620145 1106 30-31 0.38774959464893755 1106 32-33 0.5052721337478303 1106 34-35 0.16843644297966875 1106 36-37 0.22229526852871118 1106 38-39 0.3742261403067815 1106 40-41 0.2678330222972747 1106 42-43 0.1854806563407152 1106 44-45 0.12983818335715114 1106 46-47 0.07310156141977586 1106 48-49 -0.18999861607577628 1106 50-51 -0.26739346474185766 1106 52-53 -0.42154966128682503 1106 54-55 -0.6173533761600538 1106 56-57 -0.73718035632632 1106 58-59 -0.7294851904360868 1106 60-61 -0.6051684899078538 1106 62-63 -0.670866389369408 1106 64-65 -0.7496847228527059 1106 66-67 -0.5166369283982277 1106 68-69 -0.462505308545758 1106 70-71 -0.5489260933885944 1106 72-73 -0.35305261361413187 1106 74-75 -0.3959152605573095 1106 76-77 -0.3069338909406909 1106 78-79 -0.0791771831081256 1106 80-81 -0.21471729330449918 1106 82-83 -0.2345692983233505 1106 84-85 -0.20793646151843603 1106 86-87 -0.30624700649532244 1106 88-89 -0.18511716990215277 1106 90-91 -0.37840485494629306 1106 92-93 -0.34830841883890074 1106 94-95 -0.15881857258962384 1106 96-97 -0.05508978992578761 1106 98-99 -0.11084953036638368 1106 100-101 -0.3900283820672712 1107 1 0.3334252602394727 1107 2 0.3519574950424982 1107 3 0.18535727886175124 1107 4 0.2118580537732626 1107 5 0.32759969927323596 1107 6 0.3744801879062365 1107 7 0.43130757677824505 1107 8 0.4098158346077838 1107 9 0.6264643888226757 1107 10-11 0.4572210258867173 1107 12-13 0.5397091971769612 1107 14-15 0.6862167054095352 1107 16-17 0.6793326372630091 1107 18-19 0.4919168569510006 1107 20-21 0.5615531648390117 1107 22-23 0.3973907251767699 1107 24-25 0.46739258900534963 1107 26-27 0.5424011581423329 1107 28-29 0.2880545489926263 1107 30-31 0.43536742362712744 1107 32-33 0.4797731987461944 1107 34-35 0.5933176395575046 1107 36-37 0.6007220631789139 1107 38-39 0.7114782476649211 1107 40-41 0.6079828701265271 1107 42-43 0.5769787802899913 1107 44-45 0.8042026293414537 1107 46-47 0.8969398076568496 1107 48-49 0.8228207357910264 1107 50-51 0.9860210263227813 1107 52-53 1.0097038083243106 1107 54-55 1.0847522102583653 1107 56-57 1.2358167183731723 1107 58-59 0.8994898519866794 1107 60-61 0.6632051926242042 1107 62-63 0.6632596229696119 1107 64-65 0.6401952335236984 1107 66-67 0.7857458331956835 1107 68-69 0.6637235735799862 1107 70-71 0.6824305294838737 1107 72-73 0.6137769197157823 1107 74-75 0.4888129384733588 1107 76-77 0.5969052214688055 1107 78-79 0.7099964490284449 1107 80-81 0.5378585853683653 1107 82-83 0.38867325751524007 1107 84-85 0.7412349658156039 1107 86-87 0.75914901147382 1107 88-89 0.7044970290261574 1107 90-91 0.6505646754701395 1107 92-93 0.6136607060513519 1107 94-95 0.5891311579126395 1107 96-97 0.4928764765954696 1107 98-99 0.5172199681591536 1107 100-101 0.3994385675490548 1108 1 -0.0833030752287911 1108 2 0.00908146248137598 1108 3 -0.004329694057815914 1108 4 0.1934212733730476 1108 5 -0.02088099432235424 1108 6 0.06327875443529507 1108 7 0.12175523593070636 1108 8 0.20993378792982043 1108 9 0.25940792524358613 1108 10-11 0.3750293479819291 1108 12-13 0.2989848736242351 1108 14-15 0.25925009671780685 1108 16-17 0.3499498637070886 1108 18-19 0.16781193108503345 1108 20-21 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-0.7808687287558271 2316 98-99 -0.9549096744641048 2316 100-101 -1.0960415989772834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2239.0 3 367.0 4 331.0 5 338.0 6 405.0 7 436.0 8 565.0 9 715.0 10 872.0 11 1240.0 12 2059.0 13 3432.0 14 3504.0 15 4102.0 16 5809.0 17 9061.0 18 8597.0 19 3882.0 20 3384.0 21 3149.0 22 3216.0 23 3498.0 24 3926.0 25 4292.0 26 4841.0 27 5689.0 28 6492.0 29 7679.0 30 9326.0 31 11117.0 32 13640.0 33 17512.0 34 24429.0 35 38954.0 36 57726.0 37 103904.0 38 134903.0 39 27712.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18428123094986 22.415012831801796 16.139471525441586 18.26123441180676 2 14.560724751129609 24.186091040665882 39.186081654643175 22.067102553561337 3 19.25616701141615 29.037229190207864 33.578537186018316 18.128066612357674 4 10.814281128508755 19.94094270165552 41.9291800496712 27.31559612016452 5 10.24864487323674 41.24711090916943 35.80501195065029 12.699232266943545 6 24.369637508726363 41.00058551085822 18.908810437106375 15.720966543309038 7 22.662472692876502 38.36228576576306 22.834948613811587 16.140292927548852 8 24.086802127635615 33.09321731688182 21.248578260428904 21.57140229505366 9 24.833024623589928 18.850248562951453 24.69433982092958 31.622386992529037 10-11 22.434512208967913 28.37080206768487 28.44211448833807 20.752571235009142 12-13 26.026448616810345 28.020557592318205 27.942673706869453 18.010320084001993 14-15 22.939813077096314 28.584847233691164 29.988270400120108 18.48706928909241 16-17 17.505364519951698 31.91277154510372 30.83226762269434 19.749596312250244 18-19 16.91561150154228 31.274615697178483 34.15732087373584 17.6524519275434 20-21 19.25612675745708 28.84599321840472 33.21085918664027 18.687020837497922 22-23 18.645326115113587 28.745171063548614 33.25086442186039 19.358638399477403 24-25 19.142896568038008 29.69645426819824 33.583213884422264 17.577435279341486 26-27 18.956968717321963 28.300469906638202 35.37610366106646 17.366457714973368 28-29 17.189303957513193 29.926522903865905 35.15871540860137 17.725457730019542 30-31 17.95309068383609 29.66399489422409 34.32667580200101 18.056238619938807 32-33 17.980347808133043 29.973158897450098 34.00476758045292 18.041725713963945 34-35 18.986876215774878 29.00297994183093 33.705888771413626 18.30425507098056 36-37 19.12879448154217 28.522836378780585 34.55848474198421 17.789884397693044 38-39 18.649101818969662 29.63648866323157 34.06060720240394 17.65380231539483 40-41 17.725129820107096 29.842238198471733 34.84160722660554 17.591024754815635 42-43 17.393479317634558 30.442757497993377 33.00153370187398 19.162229482498088 44-45 18.25939081033719 29.694745668497564 32.801273777333925 19.24458974383132 46-47 19.240571926601184 30.291475278543327 31.621605069135278 18.846347725720207 48-49 18.728261822129287 29.8885516425134 32.16444640028496 19.218740135072355 50-51 18.05373003826284 30.425036957892026 32.91056708340282 18.610665920442308 52-53 18.121745717963165 31.592094179369713 31.536690116028172 18.749469986638953 54-55 17.684804154325036 30.254143396567706 32.07948010642067 19.981572342686594 56-57 18.979538398939056 30.8375467177045 30.678838386882873 19.504076496473566 58-59 19.374264892143376 29.822519420031096 31.28769708424089 19.515518603584642 60-61 18.592882518059156 30.385506738884466 32.17297254556442 18.848638197491958 62-63 18.841690985717545 29.976676318927787 32.13110244898652 19.050530246368155 64-65 18.454972828877995 30.66091740110833 31.98277104925667 18.901338720757003 66-67 18.337917977743036 30.030504612444414 30.955632139064093 20.67594527074846 68-69 17.82070905087915 30.132965889555752 31.850834665379857 20.19549039418524 70-71 19.01035261077105 30.395397164223414 30.89639378560624 19.697856439399295 72-73 18.709302303666302 28.918428388074386 32.21354004700437 20.158729261254944 74-75 18.059529329277257 31.199369724933185 31.034167543095815 19.706933402693746 76-77 18.72692205862539 29.640674273966432 31.248345266260202 20.38405840114797 78-79 18.290565764027768 29.457201502273474 32.144582989117275 20.107649744581483 80-81 18.730270873662942 30.038292520947845 31.097860234653552 20.13357637073566 82-83 18.167095181073638 29.810282273070293 31.77029994752308 20.252322598332988 84-85 18.132329886339654 29.372399680424493 31.215705930236066 21.27956450299979 86-87 18.312258390327226 29.6536272425005 31.51207259290107 20.5220417742712 88-89 19.05106063804125 28.816638523700398 31.066725214087565 21.065575624170787 90-91 18.42169249240872 28.2311707062387 32.369006847701314 20.97812995365126 92-93 19.02400180932367 29.263454488913183 31.929474071072494 19.783069630690655 94-95 18.62493509088219 28.702128642096543 31.717869868881227 20.95506639814004 96-97 18.27191115852406 28.213520354792927 31.74081117605261 21.77375731063041 98-99 18.89311160761823 28.505707266646624 30.75977839992989 21.84140272580525 100-101 19.28145405206773 27.36024347268941 32.14075058182812 21.217551893414743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2821.0 1 1688.0 2 663.5 3 757.5 4 614.0 5 587.5 6 1025.0 7 1899.0 8 2913.0 9 3304.5 10 2586.0 11 1836.0 12 1718.0 13 1735.5 14 1694.0 15 1616.5 16 1683.5 17 1717.0 18 1714.0 19 1804.5 20 1976.0 21 2202.0 22 2453.0 23 2701.0 24 2994.5 25 3738.0 26 5218.5 27 6843.0 28 7254.5 29 7678.5 30 9223.0 31 10695.0 32 12688.0 33 15561.5 34 18935.5 35 20684.0 36 22271.5 37 25375.0 38 24843.0 39 22299.0 40 21020.0 41 19833.5 42 18770.5 43 18242.5 44 17568.0 45 16394.5 46 15990.0 47 20041.0 48 36627.0 49 35214.0 50 16210.5 51 8987.5 52 5937.5 53 4519.5 54 3560.5 55 2956.0 56 2470.5 57 2047.0 58 1757.0 59 1496.0 60 1225.5 61 1040.5 62 807.0 63 491.5 64 280.5 65 206.0 66 152.5 67 121.0 68 107.0 69 97.0 70 84.0 71 76.0 72 69.0 73 60.5 74 64.5 75 58.5 76 37.5 77 23.0 78 22.5 79 22.5 80 15.0 81 9.5 82 9.5 83 7.0 84 4.0 85 2.5 86 1.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34742989997093815 2 0.11962238326036617 3 0.11081007602958684 4 0.12037236685447507 5 0.13837197311308816 6 0.0894355435974838 7 0.09693537953857259 8 0.10256025649438918 9 0.09037302309011991 10-11 0.08971678744527464 12-13 0.09384169721287347 14-15 0.09468542875624596 16-17 0.08352942279387639 18-19 0.09927907827016284 20-21 0.10865387319652381 22-23 0.116059961188349 24-25 0.1294659179330452 26-27 0.12862218638967274 28-29 0.12412228482501944 30-31 0.11530997759424014 32-33 0.10874762114578744 34-35 0.3358051542622505 36-37 0.5042702190889574 38-39 0.4781145412444103 40-41 0.45336508263881725 42-43 0.48805182386635293 44-45 0.49498917211186 46-47 0.5029577477992669 48-49 0.5138325099138457 50-51 0.5012702847125219 52-53 0.5058639342264388 54-55 0.4914267500398428 56-57 0.4681772586224676 58-59 0.4472714659366826 60-61 0.4076160833981757 62-63 0.4788645248385192 64-65 0.5112075673344646 66-67 0.5192698909711351 68-69 0.5311758805276134 70-71 0.5254572556225332 72-73 0.5167386963410175 74-75 0.5218010856012525 76-77 0.5007077970169402 78-79 0.4780207932951467 80-81 0.48045823997600057 82-83 0.4941454405684876 84-85 0.49517666801038723 86-87 0.4986453421331408 88-89 0.5101763398925648 90-91 0.5253635076732697 92-93 0.5174886799351264 94-95 0.5241447843328427 96-97 0.5216135897027252 98-99 0.5155199730005906 100-101 0.5043639670382211 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 533345.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.63304086236614 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24364189389036 25.643055233128248 2 11.66236736406699 7.844817562151807 3 4.14662657305764 4.183909829178626 4 1.9308917691304697 2.597670470878861 5 1.122423398107687 1.8875256006715846 6 0.715894484796064 1.4446625076173145 7 0.5016704968391458 1.1810893023744158 8 0.3750665263246521 1.0091702244786198 9 0.2979732830113164 0.9019572843071696 >10 2.4205204866934484 16.863720760088334 >50 0.33493036154246897 7.803212897481372 >100 0.22068622823763331 13.601454418032535 >500 0.011703057558056314 2.803811052397766 >1k 0.013932211378638468 8.885451350355538 >5k 0.0016718653654366163 3.3484915068578176 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6708 1.2577224873205899 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5579 1.0460396178833589 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5578 1.0458521219848316 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4053 0.7599208767308215 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3631 0.6807976075523349 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3382 0.6341111288190571 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3010 0.5643626545669314 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2959 0.5548003637420431 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2756 0.5167386963410175 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2644 0.49573915570596894 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1838 0.34461746149302985 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1797 0.33693012965341385 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1744 0.3269928470314712 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1678 0.3146181177286747 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1628 0.3052433228023137 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1557 0.2919311140068811 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1493 0.27993137650113903 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1490 0.2793688888055574 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1402 0.26286924973516207 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1355 0.2540569425043827 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1344 0.2519944876205833 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.22968247569584416 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1176 0.2204951766680104 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1060 0.1987456524388529 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1054 0.19762067704768957 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1049 0.19668319755505348 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1048 0.19649570165652624 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1033 0.19368326317861798 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 970 0.18187102157140314 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 959 0.1798085666876037 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 945 0.17718362410822264 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 930 0.17437118563031434 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 926 0.17362120203620546 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 824 0.15449662038642906 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 761 0.1426843787792142 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 734 0.13762198951897928 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 714 0.13387207154843486 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 710 0.133122087954326 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 653 0.12243482173827447 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 641 0.12018487095594783 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 619 0.116059961188349 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 604 0.11324752271044071 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 591 0.11081007602958684 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 578 0.10837262934873301 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 577 0.10818513345020578 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 572 0.10724765395756967 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.1064976703634608 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 551 0.10331024008849807 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.8749589852721973E-4 0.0 7 0.0 0.0 0.0 1.8749589852721973E-4 0.0 8 0.0 0.0 0.0 1.8749589852721973E-4 0.0 9 0.0 0.0 0.0 1.8749589852721973E-4 0.0 10-11 0.0 0.0 0.0 1.8749589852721973E-4 0.0 12-13 0.0 0.0 0.0 1.8749589852721973E-4 0.0 14-15 0.0 0.0 0.0 2.812438477908296E-4 0.0 16-17 0.0 0.0 0.0 3.7499179705443946E-4 0.0 18-19 0.0 0.0 0.0 3.7499179705443946E-4 0.0 20-21 0.0 0.0 0.0 6.56235644845269E-4 0.0 22-23 0.0 0.0 0.0 0.0022499507823266368 0.0 24-25 0.0 0.0 0.0 0.004968641310971323 0.0 26-27 0.0 0.0 0.0 0.0133122087954326 0.0 28-29 0.0 0.0 0.0 0.02596818194601993 0.0 30-31 0.0 0.0 0.0 0.058311224441965334 0.0 32-33 0.0 0.0 0.0 0.09524791645182762 0.0 34-35 0.0 0.0 0.0 0.15149668600999353 0.0 36-37 0.0 0.0 0.0 0.237651051383251 0.0 38-39 0.0 0.0 0.0 0.3615858403097432 0.0 40-41 0.0 0.0 0.0 0.5125200386241551 0.0 42-43 0.0 0.0 0.0 0.6661729274672117 0.0 44-45 0.0 0.0 0.0 0.8476689572415603 0.0 46-47 0.0 0.0 0.0 1.082226326299112 0.0 48-49 0.0 0.0 0.0 1.3318771151881053 0.0 50-51 0.0 0.0 0.0 1.5909026990034594 0.0 52-53 0.0 0.0 0.0 1.825272572162484 0.0 54-55 0.0 0.0 0.0 2.0902042767814453 0.0 56-57 0.0 0.0 0.0 2.4141034414872173 0.0 58-59 0.0 0.0 0.0 2.7589083988787744 0.0 60-61 0.0 0.0 0.0 3.1590246463358618 0.0 62-63 0.0 0.0 0.0 3.5335477036439826 0.0 64-65 0.0 0.0 0.0 3.929351545434944 0.0 66-67 0.0 0.0 0.0 4.377091751117944 0.0 68-69 0.0 0.0 0.0 4.886705603314928 0.0 70-71 0.0 0.0 0.0 5.458943085620002 0.0 72-73 0.0 0.0 0.0 6.024805707375151 0.0 74-75 0.0 0.0 0.0 6.6317299309077615 0.0 76-77 0.0 0.0 0.0 7.252997590677704 0.0 78-79 0.0 0.0 0.0 7.9909814472808405 0.0 80-81 0.0 0.0 0.0 8.778839212892219 0.0 82-83 0.0 0.0 0.0 9.543166243238428 0.0 84-85 0.0 0.0 0.0 10.364492026736915 0.0 86-87 0.0 0.0 0.0 11.222754502245262 0.0 88-89 0.0 0.0 0.0 12.089641789085864 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACAC 60 8.439857E-5 39.458916 3 GACATAT 195 0.0 38.93231 1 GGGTCGC 25 0.0016041718 38.055714 92-93 CCCTTAG 50 0.0016236214 37.958996 1 GTAAGCG 35 2.0356303E-4 33.978313 94-95 CAGGGTA 100 1.3084409E-6 33.214123 1 CATTCCG 205 0.0 32.318844 9 ACTGCGA 60 0.0040214676 31.549349 8 GACTGCG 60 0.0040214676 31.549349 7 CATATAG 255 0.0 29.710243 3 CTACACT 80 4.573989E-4 29.594183 4 ATGGGAT 645 0.0 28.630653 5 ACCCTTG 85 6.543097E-4 27.837662 7 GATAAGC 70 0.008431863 27.11357 1 TTAGGCA 245 0.0 27.05754 4 GGATATG 320 0.0 26.689919 1 TGACCGT 90 9.1070624E-4 26.305943 5 CCTTTAG 285 0.0 24.973028 1 GAGTACT 3880 0.0 24.941517 12-13 CTGACCG 95 0.0012483901 24.92142 4 >>END_MODULE