##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939174_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 523473 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23943355244683 33.0 31.0 34.0 31.0 34.0 2 32.31945105096156 34.0 31.0 34.0 31.0 34.0 3 32.3338567605206 34.0 31.0 34.0 30.0 34.0 4 35.78324574524378 37.0 35.0 37.0 35.0 37.0 5 35.85213181959719 37.0 35.0 37.0 35.0 37.0 6 35.96741952306996 37.0 37.0 37.0 35.0 37.0 7 35.93742752730322 37.0 37.0 37.0 35.0 37.0 8 35.94735545099747 37.0 37.0 37.0 35.0 37.0 9 37.611718273912885 39.0 38.0 39.0 35.0 39.0 10-11 37.55278495739035 39.0 38.0 39.0 35.0 39.0 12-13 37.49322601165676 39.0 38.0 39.0 35.0 39.0 14-15 38.710169387914945 40.0 38.0 41.0 35.0 41.0 16-17 38.65423336065088 40.0 38.0 41.0 35.0 41.0 18-19 38.70298563631744 40.0 38.5 41.0 35.0 41.0 20-21 38.74155591596892 40.5 38.5 41.0 35.0 41.0 22-23 38.76703382982504 41.0 39.0 41.0 35.0 41.0 24-25 38.696422738135496 41.0 38.5 41.0 35.0 41.0 26-27 38.55342109335152 40.5 38.0 41.0 35.0 41.0 28-29 38.42757410601884 40.0 38.0 41.0 34.5 41.0 30-31 38.18939563263053 40.0 38.0 41.0 34.0 41.0 32-33 37.897097844587975 40.0 37.0 41.0 34.0 41.0 34-35 37.573219631193965 40.0 36.5 41.0 33.5 41.0 36-37 37.26316543546659 40.0 36.0 41.0 33.0 41.0 38-39 37.03348310228034 40.0 35.5 41.0 33.0 41.0 40-41 36.85865555625601 40.0 35.5 41.0 32.0 41.0 42-43 36.60244367904362 40.0 35.0 41.0 31.0 41.0 44-45 36.20772513577586 40.0 35.0 41.0 30.0 41.0 46-47 35.74235060834083 40.0 35.0 41.0 28.0 41.0 48-49 35.43021130029629 40.0 35.0 41.0 24.5 41.0 50-51 34.2580247691858 38.5 34.0 40.0 22.0 40.5 52-53 34.38336361187683 38.5 35.0 40.0 22.0 40.5 54-55 34.92974327233688 39.5 35.0 41.0 22.0 41.0 56-57 34.86214761792871 40.0 35.0 41.0 19.5 41.0 58-59 34.70533437254643 39.0 35.0 41.0 18.5 41.0 60-61 34.39603857314513 39.0 35.0 41.0 12.5 41.0 62-63 34.04267937410334 39.0 35.0 41.0 8.0 41.0 64-65 33.6752258473694 38.0 34.5 40.0 4.0 41.0 66-67 33.25142175432162 37.5 34.0 40.0 2.0 41.0 68-69 32.81281269521064 37.0 34.0 39.5 2.0 41.0 70-71 32.33353487190361 36.5 33.5 39.0 2.0 41.0 72-73 31.848906247313614 36.0 33.0 39.0 2.0 40.0 74-75 31.38078277198633 35.0 32.5 37.5 2.0 40.0 76-77 30.91906650390756 35.0 32.0 37.0 2.0 39.0 78-79 30.45010439888972 35.0 31.5 36.5 2.0 39.0 80-81 29.97759578813043 35.0 31.0 36.0 2.0 38.5 82-83 29.569412367017975 35.0 31.0 36.0 2.0 37.0 84-85 29.160868850924498 35.0 30.0 35.0 2.0 37.0 86-87 28.79400083671937 34.0 30.0 35.0 2.0 36.5 88-89 28.316979099208556 34.0 29.0 35.0 2.0 36.0 90-91 27.863190651666848 34.0 29.0 35.0 2.0 36.0 92-93 27.487141648184338 34.0 27.5 35.0 2.0 35.0 94-95 27.04002403180298 34.0 26.5 35.0 2.0 35.0 96-97 26.458585256546183 34.0 25.0 35.0 2.0 35.0 98-99 25.867314073505224 34.0 22.0 35.0 2.0 35.0 100-101 23.252068397032893 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24204914337373395 1101 2 0.3769619701041691 1101 3 0.22986282135152436 1101 4 0.22717502602515793 1101 5 0.12450767311118938 1101 6 0.20571374782425522 1101 7 0.12101721906542906 1101 8 0.34810889056088 1101 9 0.1824539816606645 1101 10-11 0.24764659082705265 1101 12-13 0.2644420577026523 1101 14-15 0.40362147292558603 1101 16-17 0.6171222036856534 1101 18-19 0.44898449962330034 1101 20-21 0.3705287389589884 1101 22-23 0.44024057520923776 1101 24-25 0.5788713992706533 1101 26-27 0.7744786279701401 1101 28-29 0.6223234825208976 1101 30-31 0.5213518293651092 1101 32-33 0.3594176484918492 1101 34-35 0.4393440348924287 1101 36-37 0.40506057344622093 1101 38-39 0.5898439893740672 1101 40-41 0.5852315415367286 1101 42-43 0.6428035356438215 1101 44-45 0.38030248159078894 1101 46-47 0.8660990019880117 1101 48-49 0.6017864244909603 1101 50-51 0.4614861058226012 1101 52-53 0.6438500662174533 1101 54-55 0.5954805823641536 1101 56-57 0.49517579431857683 1101 58-59 0.5212314908292157 1101 60-61 0.48817833980067604 1101 62-63 0.2857744153608195 1101 64-65 0.07663857390517848 1101 66-67 0.19789461444235457 1101 68-69 0.4299845821826196 1101 70-71 0.21518404015180437 1101 72-73 0.2239136555920851 1101 74-75 0.2349081682705183 1101 76-77 0.11153965848077618 1101 78-79 -0.09363291669025031 1101 80-81 -0.19921445379486968 1101 82-83 -0.19633534783649154 1101 84-85 -0.05930829585061659 1101 86-87 -0.2299619106802524 1101 88-89 -0.37333152738862196 1101 90-91 -0.6263259851598555 1101 92-93 -0.5369923629729989 1101 94-95 -0.4808498584366063 1101 96-97 -0.4022504215837266 1101 98-99 -0.7721623698502675 1101 100-101 -1.2948425402919632 1102 1 0.07454063909582942 1102 2 0.19170271848062015 1102 3 0.4067054148985818 1102 4 0.18125707942171232 1102 5 0.15372115625725513 1102 6 0.08037615376179019 1102 7 0.06307473890874604 1102 8 0.15495869985888788 1102 9 0.3589203253365767 1102 10-11 0.38111478998178683 1102 12-13 0.41156864259080805 1102 14-15 0.37558570341729336 1102 16-17 0.4029484071698377 1102 18-19 0.4616867983553945 1102 20-21 0.381563683810036 1102 22-23 0.3744406576749668 1102 24-25 0.4252557926321714 1102 26-27 0.24650728480964545 1102 28-29 0.37618390928102485 1102 30-31 0.358760343951225 1102 32-33 0.3253206481826041 1102 34-35 0.20305138465018757 1102 36-37 0.25678719956723484 1102 38-39 0.11204530882567809 1102 40-41 0.23432184150580326 1102 42-43 0.39452758471093574 1102 44-45 0.372236302846332 1102 46-47 0.23802715372494276 1102 48-49 0.2357803426437286 1102 50-51 0.30469556876457204 1102 52-53 0.4293670232321958 1102 54-55 0.4931019616034078 1102 56-57 0.5895346233052834 1102 58-59 0.44009294841090707 1102 60-61 0.21926791819465308 1102 62-63 0.3978453358844689 1102 64-65 0.476306133950537 1102 66-67 0.46270236622051897 1102 68-69 0.42925785297944685 1102 70-71 0.4926734742307701 1102 72-73 0.2010088004225139 1102 74-75 0.2637685950306441 1102 76-77 0.2242780359676324 1102 78-79 0.3442343134922048 1102 80-81 0.3840733515860215 1102 82-83 0.016812781222370177 1102 84-85 0.20102569033637252 1102 86-87 0.3438913003289201 1102 88-89 0.21547890452561447 1102 90-91 0.4884121861626909 1102 92-93 0.41645315810730565 1102 94-95 0.6577312156794619 1102 96-97 0.7633433316375928 1102 98-99 0.6922949025958687 1102 100-101 0.18241753598882937 1103 1 0.3042015935373499 1103 2 0.35720809059480274 1103 3 0.4872404135463313 1103 4 0.41971010722885893 1103 5 0.2601750691724618 1103 6 0.3848336562818844 1103 7 0.44305162775392404 1103 8 0.3855539350271826 1103 9 0.3850015094144652 1103 10-11 0.4418504131224452 1103 12-13 0.5294306149065875 1103 14-15 0.5295650017232916 1103 16-17 0.58856540124453 1103 18-19 0.6299237122786323 1103 20-21 0.5538794706956836 1103 22-23 0.5412859850420091 1103 24-25 0.5905325118316398 1103 26-27 0.7557837089730981 1103 28-29 0.5437496162610884 1103 30-31 0.6012406134174597 1103 32-33 0.7549594749227069 1103 34-35 0.6395252124966646 1103 36-37 0.46277643075254815 1103 38-39 0.42730358827395776 1103 40-41 0.599758371882217 1103 42-43 0.7118191540566912 1103 44-45 0.9247035415550542 1103 46-47 0.7202610681153345 1103 48-49 0.6801168803960493 1103 50-51 0.6034244552494599 1103 52-53 0.717435274897916 1103 54-55 0.8621403900448783 1103 56-57 0.9360421344306005 1103 58-59 1.0152164947438536 1103 60-61 1.1623898910021637 1103 62-63 1.0294658212187215 1103 64-65 1.0857300761234754 1103 66-67 1.0413491343467953 1103 68-69 1.303606929762573 1103 70-71 1.1242865836645421 1103 72-73 1.2787286637224895 1103 74-75 1.1079207352710974 1103 76-77 1.3607592397145858 1103 78-79 1.2735854801137982 1103 80-81 1.1858632367678332 1103 82-83 1.2277758279609934 1103 84-85 1.1510785509564414 1103 86-87 0.9732129633486153 1103 88-89 0.7066425757571402 1103 90-91 0.7448697870281329 1103 92-93 0.3109163154844339 1103 94-95 0.14583142958057493 1103 96-97 0.2781467638899464 1103 98-99 -0.10419416877826038 1103 100-101 0.0521050436720607 1104 1 0.06364224492116222 1104 2 0.18282486729674474 1104 3 0.2090828079641156 1104 4 0.19188542489116145 1104 5 0.2403875014373682 1104 6 0.27885230749665624 1104 7 0.2849342171360689 1104 8 0.2712002005581198 1104 9 0.31355214286080724 1104 10-11 0.3413043063052683 1104 12-13 0.23991652319070056 1104 14-15 0.34799974594995575 1104 16-17 0.44897242221540523 1104 18-19 0.5334088410809557 1104 20-21 0.49435241892118853 1104 22-23 0.5440963455758165 1104 24-25 0.5498374582540038 1104 26-27 0.7315798603991723 1104 28-29 0.6975587465284576 1104 30-31 0.7276950982551327 1104 32-33 0.5237726904259361 1104 34-35 0.5098001911313546 1104 36-37 0.5260788039458859 1104 38-39 0.13297408347967377 1104 40-41 0.2922786337129679 1104 42-43 0.44120984347546255 1104 44-45 0.46442659073753845 1104 46-47 0.11794469242389027 1104 48-49 0.0640703556639366 1104 50-51 0.09902695710425746 1104 52-53 0.23008072338168262 1104 54-55 0.0017145863803804673 1104 56-57 -0.15940447106709854 1104 58-59 -0.03854427008742789 1104 60-61 0.03742628247106694 1104 62-63 -0.09354203933855842 1104 64-65 -0.048883141258023954 1104 66-67 -0.10404065350267189 1104 68-69 -0.01224284625380534 1104 70-71 -0.04674158098454484 1104 72-73 0.032621376976493366 1104 74-75 0.007031844295244838 1104 76-77 0.06017040226941006 1104 78-79 -0.06107985924251835 1104 80-81 -0.04461450104454556 1104 82-83 -0.09940460798532413 1104 84-85 -0.3983739335243577 1104 86-87 -0.20482114602301138 1104 88-89 -0.26374181206791647 1104 90-91 0.06937134129602995 1104 92-93 -0.10184746396917532 1104 94-95 -0.2274980846050525 1104 96-97 -0.1594806062512042 1104 98-99 -0.35597148116262645 1104 100-101 -0.3475554591406471 1105 1 -0.06940496537926322 1105 2 -0.13691762197363744 1105 3 0.03311714272806654 1105 4 0.08673521029889031 1105 5 0.0494003769738498 1105 6 0.010611964149020991 1105 7 -0.12708295024590655 1105 8 0.011543548197607834 1105 9 0.12256501839728884 1105 10-11 -0.058910285969410836 1105 12-13 -0.04871008625136142 1105 14-15 -0.3140174214320197 1105 16-17 0.012277143245448485 1105 18-19 -0.05457399153706888 1105 20-21 -0.020669040306280806 1105 22-23 -9.6803210658436E-4 1105 24-25 0.1238717930179547 1105 26-27 0.08780303636483211 1105 28-29 -0.09106786291360436 1105 30-31 0.027051321431095232 1105 32-33 -0.04918868296461909 1105 34-35 0.1546499765390763 1105 36-37 0.11406163656391044 1105 38-39 0.07825305343676092 1105 40-41 0.08519708864858444 1105 42-43 -0.08776011360608038 1105 44-45 -0.18793392428392508 1105 46-47 -0.037634706717739164 1105 48-49 0.27007893935492433 1105 50-51 0.19452051933600956 1105 52-53 0.18287042295249734 1105 54-55 0.1540751014018369 1105 56-57 0.2805096920230312 1105 58-59 0.31982482862501627 1105 60-61 0.5374262824710669 1105 62-63 0.29379701645542866 1105 64-65 0.3395288759093589 1105 66-67 0.25647436795654954 1105 68-69 0.16694170310241674 1105 70-71 0.14961035034592385 1105 72-73 0.12704197783486393 1105 74-75 0.2581048056686335 1105 76-77 0.2500845653595398 1105 78-79 0.3863450334613425 1105 80-81 0.33521382513571396 1105 82-83 0.12806320317347186 1105 84-85 0.21535997205504032 1105 86-87 0.014062974148661311 1105 88-89 0.08282471153723137 1105 90-91 0.17988636275525494 1105 92-93 0.11059888796215134 1105 94-95 0.11584955487992943 1105 96-97 0.17421038087325869 1105 98-99 0.25668946304337936 1105 100-101 -0.21558121021361032 1106 1 0.17224459610608278 1106 2 0.2990467992492256 1106 3 0.3505582076033562 1106 4 0.33428939151606585 1106 5 0.3004911099641916 1106 6 0.16374554450782597 1106 7 0.39618009634239115 1106 8 0.3203342785222034 1106 9 0.3604603258018315 1106 10-11 0.45629333233179636 1106 12-13 0.4669156079524228 1106 14-15 0.43321242777581404 1106 16-17 0.5029181654688557 1106 18-19 0.485945295110227 1106 20-21 0.4061385552828014 1106 22-23 0.4393881999551894 1106 24-25 0.41856074529226817 1106 26-27 0.541554624703096 1106 28-29 0.42689924182342054 1106 30-31 0.38824832869079984 1106 32-33 0.5209914323731724 1106 34-35 0.5906988129424633 1106 36-37 0.45402822592404135 1106 38-39 0.510212196489384 1106 40-41 0.7947377812084966 1106 42-43 0.6343628837548323 1106 44-45 0.49769283621688487 1106 46-47 0.5733401553300865 1106 48-49 0.6552676294978923 1106 50-51 0.7107904346542568 1106 52-53 0.5961360464252508 1106 54-55 0.6272843888488566 1106 56-57 0.5965134773774423 1106 58-59 0.5559651888556942 1106 60-61 0.6468483494878328 1106 62-63 0.47923273207401706 1106 64-65 0.48670230023336813 1106 66-67 0.5427195807272298 1106 68-69 0.6776957528096261 1106 70-71 0.6345977769269311 1106 72-73 0.6724862238295799 1106 74-75 0.6515439689809064 1106 76-77 0.6178481850824831 1106 78-79 0.6203061491634543 1106 80-81 0.45824228638025843 1106 82-83 0.3861337387211492 1106 84-85 0.19612886106240524 1106 86-87 0.35196583449041086 1106 88-89 0.5109496282327797 1106 90-91 0.34911658516704236 1106 92-93 0.41822113447225107 1106 94-95 0.38053113193321764 1106 96-97 0.5887533393143336 1106 98-99 0.7154437615532316 1106 100-101 0.4903873873392399 1107 1 -0.26055388560543946 1107 2 -0.22157086810756965 1107 3 -0.1492692596154157 1107 4 -0.12257741317682758 1107 5 0.01736481596833528 1107 6 0.014418422354616212 1107 7 -0.017726045824623782 1107 8 -0.14653942748193316 1107 9 -0.028773628649510385 1107 10-11 -0.1927749906495677 1107 12-13 -0.23179902282742404 1107 14-15 -0.0996550670482037 1107 16-17 -0.08270122721575035 1107 18-19 -0.09656850751227353 1107 20-21 0.12249466294326083 1107 22-23 0.09652243045898246 1107 24-25 0.06775846785956219 1107 26-27 0.1811336447169083 1107 28-29 0.12966839296213806 1107 30-31 0.07932838390129149 1107 32-33 0.11597072843942868 1107 34-35 -0.11207033819626133 1107 36-37 -0.19607529374469124 1107 38-39 -0.28762251710619324 1107 40-41 0.00850589715052763 1107 42-43 -0.02691213349844901 1107 44-45 -0.03935857855192637 1107 46-47 0.06639571088564367 1107 48-49 0.2171206315682852 1107 50-51 0.1346603449372168 1107 52-53 -0.1407083290060811 1107 54-55 -0.061811539377508495 1107 56-57 0.11605281485156382 1107 58-59 -0.05708691604424132 1107 60-61 -0.41806710944936043 1107 62-63 -0.36044118096945965 1107 64-65 -0.40706063972522344 1107 66-67 -0.12450781747842399 1107 68-69 -0.20317036175213588 1107 70-71 -0.14585425895988635 1107 72-73 0.20250692776333068 1107 74-75 -0.024612000653320365 1107 76-77 0.005270545330926524 1107 78-79 -0.06952304338312487 1107 80-81 0.009182590037955407 1107 82-83 0.0509888111849186 1107 84-85 0.1341895926013983 1107 86-87 -0.16265631871869957 1107 88-89 -0.03373636210538322 1107 90-91 -0.10197037135469955 1107 92-93 -0.060082868288272806 1107 94-95 0.2829207109532277 1107 96-97 0.19020261467665023 1107 98-99 0.12124086300250525 1107 100-101 0.037143771052146946 1108 1 0.29061159370964873 1108 2 0.368058553214766 1108 3 0.24868099660255183 1108 4 0.3542067388041801 1108 5 0.3981297203552856 1108 6 0.3549227343156218 1108 7 0.1893730667863025 1108 8 0.3035918061138645 1108 9 0.46438347671232094 1108 10-11 0.431006411675142 1108 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2316 50-51 -1.4594348134513524 2316 52-53 -1.262037551752094 2316 54-55 -1.0353603084786371 2316 56-57 -1.1487256575129905 2316 58-59 -0.8612547213777546 2316 60-61 -0.8766552194901251 2316 62-63 -0.95558106619184 2316 64-65 -1.0421421113382578 2316 66-67 -0.8719130803966024 2316 68-69 -0.9285210711415814 2316 70-71 -0.9663064235655661 2316 72-73 -1.0473735122175398 2316 74-75 -0.7300218068522248 2316 76-77 -0.7300833523256962 2316 78-79 -0.37601942242254793 2316 80-81 -0.46201340868674023 2316 82-83 -0.5339585824066653 2316 84-85 -0.4264387237657985 2316 86-87 -0.711201956485997 2316 88-89 -0.9198184372118483 2316 90-91 -0.9266185232984014 2316 92-93 -1.2798697667875096 2316 94-95 -1.167523084451112 2316 96-97 -1.2480360954415417 2316 98-99 -1.7042698290792053 2316 100-101 -2.131225202553555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2148.0 3 376.0 4 324.0 5 340.0 6 359.0 7 389.0 8 521.0 9 687.0 10 869.0 11 1221.0 12 1962.0 13 3705.0 14 3411.0 15 4163.0 16 5848.0 17 9598.0 18 7717.0 19 4003.0 20 3310.0 21 3121.0 22 3192.0 23 3440.0 24 3872.0 25 4337.0 26 4741.0 27 5392.0 28 6242.0 29 7400.0 30 8983.0 31 10863.0 32 13474.0 33 17160.0 34 24156.0 35 38896.0 36 56580.0 37 103520.0 38 131254.0 39 25898.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27323774036479 22.26189814113419 16.161374022671527 18.30349009582949 2 14.63015836286342 24.19389076194319 39.10057696022092 22.07537391497247 3 19.30591200645628 28.833701152414793 33.62656864957487 18.233818191554057 4 10.906708745756228 19.94434084062545 41.97159661454597 27.177353799072346 5 10.244065721772728 41.22797957192862 35.77112143991125 12.756833266387407 6 24.424601809158965 41.013847789018115 18.84668027741457 15.714870124408353 7 22.66131344995698 38.383328553675554 22.874295000477964 16.081062995889496 8 23.91538336535612 33.113475380688826 21.357383881544358 21.613757372410696 9 24.79921847667952 18.93932297292389 24.68585171321459 31.575606837182 10-11 22.42023531525917 28.340395594983992 28.48043006353646 20.75893902622038 12-13 25.94355719845027 27.98777579266643 27.96101024642465 18.107656762458646 14-15 22.96340689423136 28.576439866642193 29.994322352725273 18.465830886401175 16-17 17.53221987610056 31.88905064277255 30.89045672265815 19.688272758468738 18-19 16.943531757829643 31.2177061097053 34.22691115302513 17.611850979439936 20-21 19.183868951000015 28.90413944604725 33.29088924195236 18.62110236100038 22-23 18.562446044403483 28.76943477001412 33.291236416096 19.376882769486397 24-25 19.044691466150102 29.723620829118392 33.593655880873754 17.63803182385776 26-27 18.90462066842215 28.326477770574815 35.36036165244879 17.408539908554253 28-29 17.209666532175564 29.982082725414422 35.12445526322331 17.683795479186703 30-31 17.963965376474437 29.590896314892817 34.33078966036134 18.114348648271406 32-33 17.946595105778247 29.977657936021586 34.022651812747306 18.05309514545286 34-35 18.898962116783423 29.00485878846541 33.77231736417913 18.323861730572034 36-37 19.116627587888843 28.512337179760454 34.57050790889172 17.80052732345898 38-39 18.592039566967497 29.67752959890638 34.00555066493741 17.724880169188722 40-41 17.745121185772117 29.88769177909965 34.81007798808777 17.557109047040463 42-43 17.36400307964649 30.40008524707251 33.108119783656754 19.12779188962424 44-45 18.21695713911283 29.648159189077354 32.84388414128933 19.290999530520487 46-47 19.172741402964068 30.35697656222248 31.623862697038906 18.846419337774538 48-49 18.666950857950443 29.816946249443138 32.209721646158805 19.30638124644761 50-51 17.878621463024977 30.40608006390365 33.020407693016686 18.69489078005469 52-53 18.12209214131689 31.50673883948716 31.571832630230784 18.799336388965163 54-55 17.73138294553103 30.171009342008126 32.10526871593709 19.99233899652375 56-57 18.932218681614003 30.824279187622377 30.767554804775983 19.47594732598764 58-59 19.35803052071007 29.80802912912999 31.34126246063988 19.492677889520056 60-61 18.626782348455418 30.29519958945435 32.16097610106329 18.91704196102694 62-63 18.85655979251017 29.934842664424366 32.15900386451728 19.049593678548185 64-65 18.596585895575913 30.658577075630124 31.88805025045535 18.856786778338613 66-67 18.340299803056798 30.029181394196563 30.995941107164565 20.634577695582077 68-69 17.83967132949065 30.099223460221303 31.767353061502796 20.293752148785252 70-71 18.949685305082173 30.5844663438731 30.75108083347578 19.714767517568944 72-73 18.733922949218844 28.924624736790054 32.13220940805727 20.20924290593383 74-75 17.994225209160927 31.335393975358716 30.916255930587283 19.754124884893073 76-77 18.76875666023444 29.488589998367942 31.378416520261514 20.364236821136103 78-79 18.310047058846113 29.433710181622384 32.0976267099983 20.158616049533205 80-81 18.778088135072615 29.9950086868047 31.120645799138035 20.10625737898465 82-83 18.124707804581625 29.831436243079708 31.8054532904924 20.238402661846262 84-85 18.08874703016631 29.358323933859705 31.166190693321173 21.386738342652812 86-87 18.31112868838847 29.759087841696992 31.51181330627627 20.417970163638266 88-89 19.054366577673328 28.740336254657272 31.056659092152746 21.148638075516658 90-91 18.46671896408857 28.287080200368898 32.28120778239408 20.964993053148458 92-93 19.104698035555504 29.338572364112643 31.914109086299973 19.642620514031883 94-95 18.61434451847435 28.692205543889365 31.61681890326279 21.0766310343735 96-97 18.296877400522355 28.218236288216314 31.612286833614995 21.872599477646336 98-99 18.87051706755005 28.51524317057948 30.741658264919103 21.872581496951366 100-101 19.423993548727747 27.22561692690958 32.07453523479463 21.275854289568045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2743.0 1 1639.0 2 615.5 3 699.0 4 586.0 5 532.0 6 986.0 7 1893.0 8 2777.0 9 3138.5 10 2549.5 11 1868.0 12 1722.5 13 1681.5 14 1701.0 15 1685.0 16 1692.5 17 1716.0 18 1658.0 19 1700.5 20 1857.0 21 2084.0 22 2347.5 23 2620.5 24 2968.0 25 3658.5 26 5174.5 27 6813.5 28 7274.5 29 7732.5 30 9038.0 31 10536.0 32 12610.0 33 15449.5 34 18741.5 35 20295.0 36 21796.0 37 24847.5 38 24285.0 39 21781.0 40 20485.5 41 19155.5 42 18198.5 43 17899.0 44 17355.0 45 16183.5 46 15792.5 47 19862.5 48 35957.0 49 34528.5 50 15875.5 51 8756.0 52 5862.0 53 4513.5 54 3524.0 55 2875.0 56 2436.5 57 1973.5 58 1657.5 59 1425.5 60 1213.0 61 986.0 62 759.5 63 503.5 64 283.5 65 215.0 66 182.5 67 147.0 68 104.0 69 78.5 70 70.5 71 81.0 72 81.0 73 72.5 74 61.5 75 47.0 76 32.5 77 22.0 78 23.5 79 23.0 80 19.0 81 10.0 82 5.0 83 3.5 84 3.0 85 3.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.387030467664999 2 0.10697781929536003 3 0.10907916931723316 4 0.12378861947034518 5 0.12665409677289946 6 0.06819835980079202 7 0.08080645993203088 8 0.07794098262947659 9 0.07488447350675202 10-11 0.07402483031598574 12-13 0.07880062582024287 14-15 0.07049074164283545 16-17 0.07402483031598574 18-19 0.08491364406569203 20-21 0.08252574631356345 22-23 0.09045356685063032 24-25 0.10162892833059202 26-27 0.1031571828919543 28-29 0.0981903555675269 30-31 0.09093114640105603 32-33 0.08949840774977888 34-35 0.33201330345595664 36-37 0.5215168690648802 38-39 0.5032733302386179 40-41 0.4768154231450333 42-43 0.5031778143285327 44-45 0.5126338894269619 46-47 0.5097684121244075 48-49 0.5152128189992607 50-51 0.5136845644378984 52-53 0.5131114689773876 54-55 0.5069029348218533 56-57 0.4838836004913339 58-59 0.47423649357273445 60-61 0.4232309975872681 62-63 0.4928620960393372 64-65 0.5198930985934327 66-67 0.5281074668607549 68-69 0.5406200510819087 70-71 0.536799414678503 72-73 0.5231406395363276 74-75 0.5275343714002442 76-77 0.5077625780126196 78-79 0.48321498912073785 80-81 0.49056971419729384 82-83 0.502700234778107 84-85 0.498688566554531 86-87 0.4963961847124876 88-89 0.5057567439008316 90-91 0.5221854804354762 92-93 0.5283940145910104 94-95 0.5229496077161573 96-97 0.5259106009287967 98-99 0.5249554418279453 100-101 0.505374680260491 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 523473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.95714785152734 #Duplication Level Percentage of deduplicated Percentage of total 1 76.51371832998348 25.98187646001367 2 11.652527332523768 7.913731869489463 3 3.9115107735991286 3.9847124898584307 4 1.9533658574373083 2.6532293291649665 5 1.1038873928730135 1.8742433705612989 6 0.7290702127292664 1.4854287004675315 7 0.5065741625190318 1.204126961409568 8 0.3812899737898932 1.035801601143071 9 0.2984875811455763 0.9122208232324588 >10 2.3739575309104364 16.700906654373163 >50 0.3314123090088683 7.866207753855681 >100 0.21662774018406497 13.385354636971424 >500 0.012941397465541545 3.1180141932273706 >1k 0.012941397465541545 8.568759269787847 >5k 0.0016880083650706362 3.315385886444104 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6440 1.2302449218966405 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5469 1.0447530245112928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5443 1.0397861971868654 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4115 0.7860959400007259 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3477 0.6642176387320836 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3257 0.6221906382946207 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3107 0.5935358652690779 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2811 0.5369904464986733 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2660 0.5081446416529601 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2618 0.5001213052058081 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1759 0.33602497167953266 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1744 0.3331594943769784 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1666 0.3182590124036961 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1650 0.3152025032809715 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1588 0.3033585304304138 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.29762757582530525 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1549 0.2959082894437727 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1548 0.2957172576236024 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1411 0.2695458982602732 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1395 0.2664893891375486 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1371 0.26190462545346177 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.23668842519098407 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1144 0.21854040227480692 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1066 0.2036399203015246 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1064 0.20325785666118404 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1050 0.20058341117880005 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 972 0.18568292920551777 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 951 0.18167126098194178 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 929 0.1774685609381955 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 911 0.17402998817513032 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 905 0.1728837972541086 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 905 0.1728837972541086 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 831 0.15874744256150747 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 781 0.14919585155299317 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 770 0.14709450153112005 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 740 0.14136354692601147 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 646 0.12340655583000461 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 639 0.1220693330888126 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 627 0.11977695124676918 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 616 0.11767560122489602 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 609 0.11633837848370403 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 590 0.11270877390046859 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.11232671026012804 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 576 0.11003432841808461 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 569 0.10869710567689261 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 561 0.1071688511155303 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 544 0.10392131017263545 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 532 0.10162892833059202 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 527 0.1006737692297406 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9103182017028575E-4 0.0 18-19 0.0 0.0 0.0 4.7757955042571445E-4 0.0 20-21 0.0 0.0 0.0 6.686113705960002E-4 0.0 22-23 0.0 0.0 0.0 0.0016237704714474291 0.0 24-25 0.0 0.0 0.0 0.0041071841336611445 0.0 26-27 0.0 0.0 0.0 0.009456075098429145 0.0 28-29 0.0 0.0 0.0 0.021873143409497718 0.0 30-31 0.0 0.0 0.0 0.051483075535892014 0.0 32-33 0.0 0.0 0.0 0.08663293044722459 0.0 34-35 0.0 0.0 0.0 0.1401218400949046 0.0 36-37 0.0 0.0 0.0 0.2287606046539172 0.0 38-39 0.0 0.0 0.0 0.3632470060537984 0.0 40-41 0.0 0.0 0.0 0.5244778622775196 0.0 42-43 0.0 0.0 0.0 0.697266143621543 0.0 44-45 0.0 0.0 0.0 0.8889665751624247 0.0 46-47 0.0 0.0 0.0 1.1204971412088112 0.0 48-49 0.0 0.0 0.0 1.368360927879757 0.0 50-51 0.0 0.0 0.0 1.620331898684364 0.0 52-53 0.0 0.0 0.0 1.8655212398729257 0.0 54-55 0.0 0.0 0.0 2.140702576828222 0.0 56-57 0.0 0.0 0.0 2.4735755234749455 0.0 58-59 0.0 0.0 0.0 2.8239278816672493 0.0 60-61 0.0 0.0 0.0 3.2211785517113585 0.0 62-63 0.0 0.0 0.0 3.6000901670191205 0.0 64-65 0.0 0.0 0.0 3.987884761964801 0.0 66-67 0.0 0.0 0.0 4.435854380264121 0.0 68-69 0.0 0.0 0.0 4.936644296840525 0.0 70-71 0.0 0.0 0.0 5.517285514248108 0.0 72-73 0.0 0.0 0.0 6.091145102039647 0.0 74-75 0.0 0.0 0.0 6.69480565377775 0.0 76-77 0.0 0.0 0.0 7.3160411329715185 0.0 78-79 0.0 0.0 0.0 8.036517642743753 0.0 80-81 0.0 0.0 0.0 8.806280362119917 0.0 82-83 0.0 0.0 0.0 9.54213493341586 0.0 84-85 0.0 0.0 0.0 10.357363225992554 0.0 86-87 0.0 0.0 0.0 11.219107766780713 0.0 88-89 0.0 0.0 0.0 12.088207032645428 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 40 1.066778E-5 35.67707 94-95 CATTCCG 235 0.0 34.231007 9 GTGTAGC 85 1.6334876E-5 33.52044 1 TAGGCAT 285 0.0 33.222427 5 CTTTAGG 285 0.0 33.222427 2 CCATAAC 80 4.497352E-4 29.679556 1 TTAGGCA 305 0.0 29.491713 4 CCCTATA 115 3.960715E-6 28.816845 2 GGATATG 320 0.0 28.195578 1 GGCATTC 290 0.0 27.738916 7 CCTTTAG 310 0.0 27.573263 1 CCTATAC 155 4.7279173E-8 27.488876 3 ATGGGTA 260 0.0 27.312668 5 ATACTCA 70 0.008544822 27.039616 6 ATATGAG 320 0.0 26.62985 3 CAGGTAT 125 7.3961237E-6 26.592882 1 GCATTCC 355 0.0 25.325838 8 ATAGGGT 170 1.15709554E-7 25.06339 3 CCGGAGA 95 0.0012497035 24.91682 4 GATTGGC 330 0.0 24.376625 9 >>END_MODULE