##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939174_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 523473 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.413996519400236 34.0 31.0 34.0 31.0 34.0 2 32.61964800476815 34.0 31.0 34.0 31.0 34.0 3 32.68893524594392 34.0 31.0 34.0 31.0 34.0 4 36.16281068937653 37.0 35.0 37.0 35.0 37.0 5 36.14618327974891 37.0 35.0 37.0 35.0 37.0 6 36.21912304932633 37.0 37.0 37.0 35.0 37.0 7 36.18900879319468 37.0 37.0 37.0 35.0 37.0 8 36.19027724448061 37.0 37.0 37.0 35.0 37.0 9 37.87841588773442 39.0 38.0 39.0 35.0 39.0 10-11 37.817522584736935 39.0 38.0 39.0 35.0 39.0 12-13 37.757802217115305 39.0 38.0 39.0 35.0 39.0 14-15 39.024721427848235 40.0 38.0 41.0 36.0 41.0 16-17 39.127634090010375 40.0 38.5 41.0 36.0 41.0 18-19 39.25931805460836 41.0 39.0 41.0 36.0 41.0 20-21 39.27772014984536 41.0 39.0 41.0 36.0 41.0 22-23 39.19894626847994 41.0 39.0 41.0 35.5 41.0 24-25 39.10099374752853 41.0 39.0 41.0 35.0 41.0 26-27 38.904027523864656 40.0 38.0 41.0 35.0 41.0 28-29 38.748262088016006 40.0 38.0 41.0 35.0 41.0 30-31 38.50497924439274 40.0 38.0 41.0 35.0 41.0 32-33 38.25416210578196 40.0 37.0 41.0 34.0 41.0 34-35 38.073508089242424 40.0 37.0 41.0 34.0 41.0 36-37 37.85790193572544 40.0 37.0 41.0 33.5 41.0 38-39 37.66121652883721 40.0 36.5 41.0 33.0 41.0 40-41 37.47142641549803 40.0 36.0 41.0 33.0 41.0 42-43 37.19869697195462 40.0 36.0 41.0 33.0 41.0 44-45 36.80386763023117 40.0 36.0 41.0 31.5 41.0 46-47 36.25848229039511 40.0 36.0 41.0 30.5 41.0 48-49 35.71346182133558 40.0 35.0 41.0 27.5 41.0 50-51 35.40639345295746 39.5 35.0 41.0 26.5 41.0 52-53 35.134920043631666 39.0 35.0 41.0 24.0 41.0 54-55 34.93471965125231 39.0 35.0 41.0 24.0 41.0 56-57 34.74275368548139 39.0 35.0 40.0 23.0 41.0 58-59 34.458781064161855 39.0 35.0 40.0 22.0 41.0 60-61 34.06724511101814 38.0 34.0 40.0 19.5 41.0 62-63 33.688516886257744 38.0 34.0 40.0 17.5 41.0 64-65 33.22298953336657 37.5 33.5 40.0 10.5 41.0 66-67 32.765265830329355 37.0 33.0 39.0 7.5 41.0 68-69 32.25652134876107 36.0 32.5 39.0 2.0 40.0 70-71 31.709461615021212 35.5 32.0 38.5 2.0 40.0 72-73 31.125814511923252 35.0 31.0 37.5 2.0 39.5 74-75 30.581861910738475 35.0 31.0 37.0 2.0 39.0 76-77 28.553426824306126 32.5 28.0 35.0 2.0 37.0 78-79 29.466567521152 34.0 29.5 36.0 2.0 38.0 80-81 29.462986629682906 34.0 30.0 36.0 2.0 37.0 82-83 29.06098213279386 34.0 29.5 35.0 2.0 37.0 84-85 28.59686937053105 34.0 29.0 35.0 2.0 36.5 86-87 27.96489599272551 34.0 28.5 35.0 2.0 36.0 88-89 27.25145805036745 33.0 26.0 35.0 2.0 35.5 90-91 26.693520964787105 33.0 25.5 35.0 2.0 35.0 92-93 26.311521320106287 33.0 25.0 35.0 2.0 35.0 94-95 25.837418548807676 32.0 24.0 34.0 2.0 35.0 96-97 25.06319237095323 32.0 19.0 34.0 2.0 35.0 98-99 24.012444767924993 32.0 2.0 34.0 2.0 35.0 100-101 18.52240421186957 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1872484503144065 1101 2 0.12901403315461835 1101 3 0.07731125798353844 1101 4 0.15303762158335843 1101 5 0.043317900363078365 1101 6 0.1685535843157595 1101 7 0.19900831377609762 1101 8 0.088517395898009 1101 9 0.08488764092837187 1101 10-11 0.10021857849791616 1101 12-13 0.15704850284075889 1101 14-15 0.24132661303137581 1101 16-17 0.17441526201984203 1101 18-19 0.242630404335209 1101 20-21 0.25009030383341724 1101 22-23 0.231987551261156 1101 24-25 0.21413818328251466 1101 26-27 0.060975347192346874 1101 28-29 0.11629851922877066 1101 30-31 0.25659490843859345 1101 32-33 0.4807222568511591 1101 34-35 0.35306994304408335 1101 36-37 0.37680473957655636 1101 38-39 0.36684295815368984 1101 40-41 0.44841228255189236 1101 42-43 0.5604798352969098 1101 44-45 0.6420462307637038 1101 46-47 1.0862927839422554 1101 48-49 0.8567275763356292 1101 50-51 0.7711712684906118 1101 52-53 0.7239471001680329 1101 54-55 0.3961899570558245 1101 56-57 0.3326800335925597 1101 58-59 0.42117956261861167 1101 60-61 0.3835619510181374 1101 62-63 0.5427846949487076 1101 64-65 0.6063960156169728 1101 66-67 0.5840393088233142 1101 68-69 0.540156131679872 1101 70-71 0.6230878242210274 1101 72-73 0.4562563357619176 1101 74-75 0.4729564392534762 1101 76-77 0.6974480891497841 1101 78-79 0.7160509149377958 1101 80-81 0.5278607991296838 1101 82-83 0.5763065322146588 1101 84-85 0.14482834035279168 1101 86-87 0.3600931455665197 1101 88-89 -0.18966360935903737 1101 90-91 0.0946713870821867 1101 92-93 0.30144399601796223 1101 94-95 -0.1319873603418351 1101 96-97 -0.2990725715451674 1101 98-99 -0.36846964276611516 1101 100-101 -0.664854699019827 1102 1 0.1431705202041087 1102 2 0.1019137342163674 1102 3 0.10826682957409872 1102 4 0.05623832254202199 1102 5 -0.030937331056364314 1102 6 0.03637648917092662 1102 7 0.2379424442781115 1102 8 0.29821742682265295 1102 9 0.29837078699578967 1102 10-11 0.22799664261501817 1102 12-13 0.153435473256188 1102 14-15 0.29218219493015596 1102 16-17 0.1841951816157632 1102 18-19 0.1741709671637821 1102 20-21 0.20916411065745422 1102 22-23 0.18062686679564166 1102 24-25 0.3008663556358826 1102 26-27 0.3992239813537708 1102 28-29 0.4204011890566264 1102 30-31 0.12341276226809583 1102 32-33 0.1934219785293365 1102 34-35 0.24646753092162044 1102 36-37 0.11656352733039199 1102 38-39 0.17009520019059465 1102 40-41 0.19293862004287377 1102 42-43 0.5739887519034426 1102 44-45 0.6781842577712354 1102 46-47 0.26667109545653034 1102 48-49 0.28946904628718784 1102 50-51 0.13223198388077861 1102 52-53 0.2547248550386598 1102 54-55 0.16308512250268592 1102 56-57 -0.12304102609200385 1102 58-59 0.10189289114145339 1102 60-61 0.2823430045177773 1102 62-63 0.0834222580865287 1102 64-65 0.2844111686939854 1102 66-67 0.40665037984691565 1102 68-69 0.3228280211758019 1102 70-71 0.3946268408172493 1102 72-73 0.29230931999034837 1102 74-75 0.23440113567652432 1102 76-77 0.410562716507787 1102 78-79 0.2394299480271691 1102 80-81 0.19117128258499605 1102 82-83 -0.036246297390533755 1102 84-85 -0.07957636019354197 1102 86-87 0.18654918102678053 1102 88-89 -0.15007748421729872 1102 90-91 0.14888229317433854 1102 92-93 -0.049831645668458435 1102 94-95 -0.023900565166083965 1102 96-97 -0.19150891831354144 1102 98-99 -0.39953345062097156 1102 100-101 -0.7936403912165098 1103 1 0.06886569882382787 1103 2 0.1695556588346676 1103 3 0.09293970838805166 1103 4 0.09575839728447733 1103 5 0.01319022284917537 1103 6 0.02870555267742958 1103 7 0.1474253936548422 1103 8 0.06387523421330599 1103 9 0.2621305293309959 1103 10-11 0.17864940980700794 1103 12-13 0.056206353201112336 1103 14-15 -0.07091617499584402 1103 16-17 0.19554177517905913 1103 18-19 0.4393837618924721 1103 20-21 0.41385026609226827 1103 22-23 0.2885239975703584 1103 24-25 0.27005230208684594 1103 26-27 0.31491006302957203 1103 28-29 0.2944748988887014 1103 30-31 0.4601963779345013 1103 32-33 0.28018223717758417 1103 34-35 0.4704539592647521 1103 36-37 0.4215520946846638 1103 38-39 0.20829498874811492 1103 40-41 0.23192762995116567 1103 42-43 0.2866507143846988 1103 44-45 0.16033211998940544 1103 46-47 0.5493696915768069 1103 48-49 0.503328363278797 1103 50-51 0.8114368618096179 1103 52-53 0.8649921084991803 1103 54-55 0.7192231010142294 1103 56-57 1.0237295588169033 1103 58-59 1.0520490143681585 1103 60-61 1.1090929369053626 1103 62-63 0.9268736877819066 1103 64-65 1.1184435734833968 1103 66-67 0.8928894081949679 1103 68-69 0.9103406216724466 1103 70-71 1.1233387627310485 1103 72-73 1.3080470329694727 1103 74-75 1.2081134263853492 1103 76-77 1.1240360280412354 1103 78-79 0.9267188128760857 1103 80-81 0.9178769595697247 1103 82-83 1.2093466020759394 1103 84-85 0.84174982671178 1103 86-87 0.9059797615101495 1103 88-89 0.8627147858390032 1103 90-91 1.1160914982981325 1103 92-93 0.74219992318557 1103 94-95 1.1588152413886341 1103 96-97 0.7505705938935741 1103 98-99 0.2633583940917319 1103 100-101 -0.2944443643682284 1104 1 -0.04175497528704142 1104 2 -0.018050580776154845 1104 3 0.10932291290340856 1104 4 0.11601553236022255 1104 5 0.11239101923538897 1104 6 -0.015414994650257086 1104 7 -0.03757474721535914 1104 8 0.12082436128761742 1104 9 0.05699064522021757 1104 10-11 0.23692271678196164 1104 12-13 0.14268657032905452 1104 14-15 0.08346347959042077 1104 16-17 0.12292788162058343 1104 18-19 0.12450129459784165 1104 20-21 0.12589748578403714 1104 22-23 0.22551040829151248 1104 24-25 0.1640239965675434 1104 26-27 0.062195963800597553 1104 28-29 0.2423764024433197 1104 30-31 0.11449151039945349 1104 32-33 0.39704111340902415 1104 34-35 0.3116674151864629 1104 36-37 0.5477107520977214 1104 38-39 0.703733142033677 1104 40-41 0.6820619262105936 1104 42-43 0.711491765839888 1104 44-45 0.7492094783913785 1104 46-47 0.8585985680899881 1104 48-49 1.0178145157379177 1104 50-51 0.9805562352189625 1104 52-53 1.0225601253284822 1104 54-55 1.1137747111606373 1104 56-57 1.2812074187167966 1104 58-59 1.276812117644802 1104 60-61 0.9651611556486088 1104 62-63 0.8069743630197408 1104 64-65 0.8203927475144468 1104 66-67 0.9385595277468113 1104 68-69 0.6398627899465978 1104 70-71 0.5918636638871213 1104 72-73 0.5406166932844592 1104 74-75 0.5809170251494464 1104 76-77 0.6251019459045146 1104 78-79 0.7464777370033921 1104 80-81 1.31200361127285 1104 82-83 1.1915711303955447 1104 84-85 0.8049850517754038 1104 86-87 0.7007436160945311 1104 88-89 1.0883465886958525 1104 90-91 0.6470594644141556 1104 92-93 0.5119757517371433 1104 94-95 0.8218654569200474 1104 96-97 0.44567582393461436 1104 98-99 0.12063340090046282 1104 100-101 -0.359129613379789 1105 1 -0.02887943880635646 1105 2 0.06349448360152365 1105 3 0.012756389298260729 1105 4 0.004427549527605379 1105 5 -0.17945447432684603 1105 6 -0.02614460838416477 1105 7 -0.140579039060853 1105 8 -0.059433149441993294 1105 9 0.0269477267652789 1105 10-11 0.0062360215029997335 1105 12-13 -0.013965790185970661 1105 14-15 -0.06138630581730098 1105 16-17 -0.10990473640516285 1105 18-19 -0.03644291141075229 1105 20-21 0.04542538277974728 1105 22-23 0.0323773610812097 1105 24-25 0.1779724944216312 1105 26-27 0.010693817877857725 1105 28-29 0.003106016177234494 1105 30-31 0.30225975074279887 1105 32-33 0.012920941735202973 1105 34-35 0.09171033364139447 1105 36-37 0.2547922971620977 1105 38-39 0.23055717636843553 1105 40-41 0.42025935110329726 1105 42-43 0.4571999203463264 1105 44-45 0.1333296500651926 1105 46-47 0.6482981389054387 1105 48-49 0.616526962089857 1105 50-51 0.5245476515279748 1105 52-53 0.6148348034400684 1105 54-55 0.4699978871263042 1105 56-57 0.4872160024077843 1105 58-59 0.41415117343878904 1105 60-61 0.41580493247264627 1105 62-63 0.5162018308309015 1105 64-65 0.5768305157547857 1105 66-67 0.3634522316094646 1105 68-69 0.20960527921698713 1105 70-71 0.2903615179643815 1105 72-73 0.061002959378878785 1105 74-75 0.13134620969880118 1105 76-77 -0.16996243177788628 1105 78-79 -0.32755659776055523 1105 80-81 -0.13864016555118752 1105 82-83 0.023116194773230347 1105 84-85 -0.34093769500571014 1105 86-87 -0.10397741394838533 1105 88-89 -0.10800534263462325 1105 90-91 -0.07182465575752062 1105 92-93 -0.3839469950439742 1105 94-95 -0.42706158170656394 1105 96-97 -0.7313628026748269 1105 98-99 -0.6014696033913864 1105 100-101 -0.4610609438518942 1106 1 -0.14674968823100443 1106 2 -0.06739791814364793 1106 3 -0.07740790930044739 1106 4 -0.060341108448746184 1106 5 0.09368639239491472 1106 6 0.1044901049152216 1106 7 0.047480211510062986 1106 8 0.10505205124398742 1106 9 0.11180275480001 1106 10-11 0.12482824286625771 1106 12-13 -0.037468753603100424 1106 14-15 -0.07023434960656516 1106 16-17 -4.071400163567773E-4 1106 18-19 -0.004627274164995754 1106 20-21 0.0953925052883875 1106 22-23 0.08448152052816482 1106 24-25 0.0955321848400601 1106 26-27 0.056022548362399505 1106 28-29 0.09934598632091252 1106 30-31 0.25463848167163405 1106 32-33 -0.06974595596248179 1106 34-35 -0.15661247000888778 1106 36-37 0.0017311361423608673 1106 38-39 -0.004787904003698884 1106 40-41 -0.08775786959352416 1106 42-43 -0.0631596883226706 1106 44-45 0.1921559236236945 1106 46-47 0.048821513024634555 1106 48-49 -0.050268548799699886 1106 50-51 0.10394901461099693 1106 52-53 0.17430751956514712 1106 54-55 0.09163794079658771 1106 56-57 -0.15775151996223968 1106 58-59 0.015524919429076078 1106 60-61 -0.21363019668257976 1106 62-63 -0.07800328917193866 1106 64-65 0.013501358751433656 1106 66-67 -0.015705216030902136 1106 68-69 -0.5447110022498833 1106 70-71 -0.4604781909365343 1106 72-73 -0.1458719906384438 1106 74-75 0.03659016957049133 1106 76-77 -0.32560403157884465 1106 78-79 0.027293720795679377 1106 80-81 -0.04261567626302565 1106 82-83 -0.08542395575236483 1106 84-85 -0.07185542130504174 1106 86-87 -0.029525446186099202 1106 88-89 0.1033991482529828 1106 90-91 0.6539815092164005 1106 92-93 0.8672525891001897 1106 94-95 0.3610274363227397 1106 96-97 -0.3885149899165832 1106 98-99 -0.23359491106250374 1106 100-101 -0.49325422795784846 1107 1 -0.07350952259952948 1107 2 -0.0641197953003072 1107 3 -0.09052040067381029 1107 4 -0.0815626405398433 1107 5 -0.050809811883901546 1107 6 -0.07917104070244108 1107 7 -0.030677138386415947 1107 8 0.022495115426181655 1107 9 -0.04606474002400773 1107 10-11 -0.07293745761683113 1107 12-13 -0.031516372650720825 1107 14-15 0.07430498786754924 1107 16-17 0.003216048389440118 1107 18-19 0.15233442355757632 1107 20-21 0.14715233965692676 1107 22-23 0.10518545427557768 1107 24-25 0.10851527662750016 1107 26-27 0.015649877554942293 1107 28-29 -0.0042599488067622815 1107 30-31 -0.036855652213809265 1107 32-33 -0.1582121395373619 1107 34-35 -0.030922338883975442 1107 36-37 -0.1593282151343871 1107 38-39 0.11594190966089712 1107 40-41 0.29681769976465233 1107 42-43 0.1302064262390914 1107 44-45 -0.007637037038833228 1107 46-47 -0.28666848007405576 1107 48-49 -0.1870870443138486 1107 50-51 -0.174993359440073 1107 52-53 -0.18779565503803042 1107 54-55 -0.0968109895001632 1107 56-57 -0.021623155573010422 1107 58-59 0.08462429831106277 1107 60-61 0.05841086611934543 1107 62-63 -0.13144531815744642 1107 64-65 -0.2197198282740942 1107 66-67 -0.3674132905650609 1107 68-69 -0.08249568133891216 1107 70-71 -0.0926814345528193 1107 72-73 -0.09842547580062799 1107 74-75 -0.032595475702112964 1107 76-77 -0.176923907355242 1107 78-79 0.04165707483294412 1107 80-81 0.34472041759480376 1107 82-83 0.0647658303069889 1107 84-85 0.1375562419109677 1107 86-87 0.28526061316517826 1107 88-89 0.28848368931578605 1107 90-91 0.6252979343371727 1107 92-93 0.5012674269193766 1107 94-95 0.7450422741419267 1107 96-97 0.7634001239550514 1107 98-99 0.6680182704419835 1107 100-101 -8.456703319019709E-4 1108 1 -0.05077798904122943 1108 2 0.057208788626702756 1108 3 0.016684948657527343 1108 4 0.008144896018087877 1108 5 0.03672612112447382 1108 6 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-0.9014987624287691 2316 98-99 -0.9629867738521583 2316 100-101 -0.04933010475607702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 5.0 5 19.0 6 41.0 7 85.0 8 172.0 9 315.0 10 580.0 11 1012.0 12 1673.0 13 2280.0 14 2843.0 15 4216.0 16 5897.0 17 9220.0 18 8876.0 19 5030.0 20 3613.0 21 3614.0 22 3626.0 23 4118.0 24 4429.0 25 5126.0 26 5740.0 27 6554.0 28 7689.0 29 9182.0 30 11185.0 31 13492.0 32 16836.0 33 21542.0 34 30376.0 35 46772.0 36 70940.0 37 124679.0 38 87659.0 39 4035.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5235760379979 22.922853218541622 16.31638314035128 18.2371876031092 2 14.784716690259097 24.67347886137394 38.753288135204684 21.788516313162283 3 19.294977964479543 29.000158556410742 33.72857816926565 17.976285309844062 4 10.9369537683892 20.532291063722486 41.33355493024473 27.19720023764358 5 10.132900837292468 41.46593998162274 35.801846513573764 12.599312667511029 6 24.28759458462996 41.45466910423269 18.51614123364529 15.741595077492057 7 22.526472234480096 38.58326981525313 22.611099330815534 16.27915861945124 8 24.05167028671966 33.49131664861416 21.05075142366464 21.406261641001542 9 24.609292169796724 19.080640262248483 24.323508566821975 31.986559001132818 10-11 22.45502633373641 28.625831704787064 28.10106729477929 20.818074666697232 12-13 26.074315198682648 28.006984123345426 27.857119660421837 18.061581017550093 14-15 22.86785164723379 28.753879173245384 29.863688072921292 18.514581106599536 16-17 17.446393110503404 32.15047467492958 30.68599816415089 19.71713405041612 18-19 16.902304416846714 31.307154332697195 34.114653477829805 17.675887772626286 20-21 19.156766442586342 28.850485125307323 33.2724896986091 18.72025873349724 22-23 18.55224624765747 28.785534306449424 33.35109929260917 19.311120153283934 24-25 19.10958158300428 29.74843019601775 33.78779803351845 17.354190187459526 26-27 18.998003648833233 28.398238626051903 35.48499871049087 17.11875901462399 28-29 17.12380581233416 29.972319489257327 35.36658051131577 17.537294187092744 30-31 17.867683720077252 29.733816261774727 34.41132589455426 17.987174123593768 32-33 17.838551367501285 29.986073780309585 34.167091712466544 18.008283139722582 34-35 18.8339205062169 28.93179751324005 34.05882937341902 18.175452607124022 36-37 19.079779570529407 28.560577977689544 34.72681344130607 17.632829010474985 38-39 18.52198602157523 29.629923305908925 34.29251898245628 17.555571690059573 40-41 17.70408148644169 29.80468052221476 34.94582313208495 17.545414859258592 42-43 17.35689476640442 30.427701336960716 33.23296893925789 18.982434957376974 44-45 18.255498074336714 29.582906681745936 32.93734334882015 19.2242518950972 46-47 19.211914887113473 30.27045769764216 31.717311814399192 18.80031560084517 48-49 18.713953581669553 29.929487999969435 32.15756021984614 19.198998198514875 50-51 17.88675972498954 30.381339176382266 33.04232708211152 18.689574016516673 52-53 18.235228942084884 31.63315013381779 31.445747918230737 18.685873005866586 54-55 17.646086808679723 30.341488481736402 31.88817761374512 20.124247095838754 56-57 19.002508247798836 30.942570103902206 30.556781343068312 19.498140305230642 58-59 19.315809079881177 30.019676578185745 31.195208848729138 19.46930549320394 60-61 18.5507789030882 30.736512115049752 31.89782228076426 18.81488670109779 62-63 18.713214013490823 30.244695463133304 31.893510001585586 19.148580521790297 64-65 18.369686173185023 31.022575853818125 31.589766122369056 19.017971850627795 66-67 18.22680707787091 30.290899539126485 30.53007617546625 20.952217207536357 68-69 17.75973926731977 30.373695190353196 31.490520548363925 20.376044993963106 70-71 18.824484571777887 30.70772581176662 30.50475430896561 19.96303530748989 72-73 18.63353028713993 29.024419597572365 31.779384992158143 20.56266512312956 74-75 17.952024268682436 31.389584563100676 30.42391871213988 20.23447245607701 76-77 18.531942558846588 29.605044266401514 31.107138076753927 20.755875097997972 78-79 17.959032774544607 29.62424101155153 31.81761732079466 20.599108893109204 80-81 18.30586765468607 30.13812595337091 30.802058946857663 20.75394744508536 82-83 17.755357009817125 30.083308976776262 31.38499979941659 20.776334213990026 84-85 17.596609567255616 29.533232850595926 30.811426759355303 22.05873082279315 86-87 17.756635814273693 29.882104337183506 31.04212881511512 21.319131033427674 88-89 18.549897799745054 28.920130332901383 30.536534552894267 21.993437314459296 90-91 17.868067338930523 28.425672895322705 31.715788352947154 21.990471412799614 92-93 18.335234099944028 29.569242348698022 31.295501391666807 20.80002215969114 94-95 17.75659871664823 28.9997764927704 31.061964991508635 22.18165979907273 96-97 17.287711114040267 28.66432461655138 31.071707614337317 22.976256655071033 98-99 17.703048109545495 28.91214811871504 30.12099978604395 23.26380398569551 100-101 17.99303496653116 27.55983128037412 31.635502949536935 22.811630803557783 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2135.0 1 1333.0 2 597.0 3 668.0 4 549.0 5 507.0 6 943.5 7 1729.5 8 2595.0 9 2928.5 10 2342.5 11 1684.0 12 1529.5 13 1517.5 14 1482.0 15 1480.0 16 1521.0 17 1539.0 18 1555.0 19 1674.5 20 1864.0 21 2139.0 22 2424.0 23 2669.5 24 2927.0 25 3561.5 26 5077.0 27 6721.0 28 7126.5 29 7495.0 30 8952.0 31 10535.5 32 12556.0 33 15487.0 34 18831.0 35 20494.5 36 22186.5 37 25024.0 38 24340.5 39 22167.5 40 20941.0 41 19438.0 42 18376.5 43 18014.0 44 17513.5 45 16580.5 46 15990.0 47 20070.0 48 35643.0 49 34385.5 50 17282.0 51 9955.0 52 6017.5 53 4236.5 54 3310.0 55 2770.5 56 2312.5 57 1872.5 58 1566.0 59 1310.5 60 1090.0 61 941.0 62 698.5 63 400.5 64 212.0 65 137.5 66 91.5 67 84.0 68 65.0 69 48.0 70 47.0 71 41.0 72 40.5 73 43.5 74 39.5 75 28.0 76 16.0 77 11.0 78 10.0 79 9.0 80 5.0 81 2.5 82 3.0 83 2.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4166403997913933 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.59643190766286E-4 16-17 0.0018148022916177147 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.003438572763065144 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011652941030387432 36-37 0.025025168442307438 38-39 0.018148022916177148 40-41 0.00926504327825886 42-43 0.00897849554800343 44-45 0.003247540942894858 46-47 0.005157859144597716 48-49 0.0030565091227245725 50-51 3.8206364034057156E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0015282545613622862 60-61 0.002387897752128572 62-63 0.0011461909210217147 64-65 0.0029609932126394294 66-67 0.0020058341117880005 68-69 0.004775795504257144 70-71 9.551591008514289E-5 72-73 0.0 74-75 0.0 76-77 0.09608900554565374 78-79 0.015091513793452575 80-81 2.8654773025542867E-4 82-83 0.0 84-85 0.0 86-87 0.10564059655416803 88-89 0.4188372657233515 90-91 0.4201744884645435 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.9103182017028578E-4 100-101 1.9103182017028578E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 523473.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.662005097987 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2825478694557 24.588789623016126 2 12.062833822369882 7.879926796048302 3 4.2592031079282 4.173423408735389 4 2.0193886322246635 2.6382912720215588 5 1.2042779138120234 1.9667065680160731 6 0.7720773735294999 1.5130557066156565 7 0.5720474350331515 1.3078951367540226 8 0.4072146891250939 1.0640358601743207 9 0.3191428643802838 0.9381461277037503 >10 2.491740254447886 16.901166735811717 >50 0.3500537838719985 7.921153475584242 >100 0.22908361148217593 13.787720189772093 >500 0.015194321169736161 3.4556148948643846 >1k 0.013441130265535835 8.52603878279951 >5k 0.0017531909042003261 3.3380354220828745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6218 1.1878358578188368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5767 1.1016805069220381 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5503 1.0512481063970827 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3773 0.7207630575024881 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3480 0.6647907341925945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3245 0.6198982564525773 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3076 0.587613878843799 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2799 0.5346980646566298 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2600 0.496682732442743 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2444 0.4668817684961784 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1934 0.36945554020933263 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1852 0.35379093095536923 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1692 0.3232258397281235 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1666 0.3182590124036961 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1602 0.3060329759127978 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1519 0.29017733483866404 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1503 0.2871208257159395 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1498 0.2861656666150881 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1469 0.28062574383014977 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1392 0.2659162936770378 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1385 0.26457907093584576 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1248 0.23840771157251664 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1222 0.2334408842480892 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1140 0.21777627499412577 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1128 0.21548389315208233 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1001 0.19122285199045605 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 997 0.1904587247097749 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 994 0.18988562924926405 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 992 0.18950356560892348 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 983 0.1877842792273909 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 930 0.17765959275836576 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 906 0.1730748290742789 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 874 0.16696181082882974 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 805 0.15378061523708006 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 727 0.13888013326379775 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 661 0.1262720331325589 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 660 0.1260810013123886 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 648 0.12378861947034518 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 621 0.11863076032574746 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 608 0.11614734666353374 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 597 0.1140459966416606 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 597 0.1140459966416606 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 582 0.11118051933910632 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 572 0.10927020113740346 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 563 0.10755091475587089 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 556 0.10621369201467888 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 555 0.10602266019450861 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 548 0.1046854374533166 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 536 0.10239305561127318 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.10220202379110288 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 530 0.10124686469025145 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 527 0.1006737692297406 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9103182017028575E-4 0.0 18-19 0.0 0.0 0.0 4.7757955042571445E-4 0.0 20-21 0.0 0.0 0.0 6.686113705960002E-4 0.0 22-23 0.0 0.0 0.0 0.0018148022916177147 0.0 24-25 0.0 0.0 0.0 0.004584763684086859 0.0 26-27 0.0 0.0 0.0 0.010029170558940002 0.0 28-29 0.0 0.0 0.0 0.022541754780093723 0.0 30-31 0.0 0.0 0.0 0.052247202816573156 0.0 32-33 0.0 0.0 0.0 0.08816118500858688 0.0 34-35 0.0 0.0 0.0 0.14222319011677775 0.0 36-37 0.0 0.0 0.0 0.23248572514723775 0.0 38-39 0.0 0.0 0.0 0.3679272856479704 0.0 40-41 0.0 0.0 0.0 0.5305908805229687 0.0 42-43 0.0 0.0 0.0 0.7048119005182694 0.0 44-45 0.0 0.0 0.0 0.8990912616314499 0.0 46-47 0.0 0.0 0.0 1.1342514322610717 0.0 48-49 0.0 0.0 0.0 1.3860313712455086 0.0 50-51 0.0 0.0 0.0 1.6416319466333507 0.0 52-53 0.0 0.0 0.0 1.889877796944637 0.0 54-55 0.0 0.0 0.0 2.167924611202488 0.0 56-57 0.0 0.0 0.0 2.5033764874215096 0.0 58-59 0.0 0.0 0.0 2.855066068355006 0.0 60-61 0.0 0.0 0.0 3.2539405088705626 0.0 62-63 0.0 0.0 0.0 3.634475894649772 0.0 64-65 0.0 0.0 0.0 4.023798744156814 0.0 66-67 0.0 0.0 0.0 4.473583164747752 0.0 68-69 0.0 0.0 0.0 4.979435424558669 0.0 70-71 0.0 0.0 0.0 5.565521048841106 9.551591008514288E-5 72-73 0.0 0.0 0.0 6.143678852586476 1.9103182017028575E-4 74-75 0.0 0.0 0.0 6.748008015695174 1.9103182017028575E-4 76-77 0.0 0.0 0.0 7.372873099472178 1.9103182017028575E-4 78-79 0.0 0.0 0.0 8.09239445014356 1.9103182017028575E-4 80-81 0.0 0.0 0.0 8.863971971811345 1.9103182017028575E-4 82-83 0.0 0.0 0.0 9.600686186298052 1.9103182017028575E-4 84-85 0.0 0.0 0.0 10.41543689932432 1.9103182017028575E-4 86-87 0.0 0.0 0.0 11.276608344651969 1.9103182017028575E-4 88-89 0.0 0.0 0.0 12.146662769617535 1.9103182017028575E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 85 0.0 41.86958 94-95 GACATAT 165 0.0 40.447918 1 CATTCCG 145 0.0 39.270782 9 CTAGATA 65 1.3350826E-4 36.501686 4 TACGCCG 70 2.0639828E-4 33.894424 5 CTTTGCG 100 1.3076497E-6 33.216534 2 CATATAG 210 0.0 31.634794 3 ACGCCGA 95 3.5113015E-5 29.969807 6 TTTACGC 80 4.516891E-4 29.657621 3 CGCCGAA 80 4.516891E-4 29.657621 7 GGTCGGG 65 0.0057396432 29.335852 1 TATATCG 65 0.005870016 29.201351 5 GCCCTGG 230 0.0 28.883944 6 AGGGGTG 135 4.2549982E-7 28.119818 8 CCCTGGC 260 0.0 27.376266 7 GGTACAT 70 0.008241895 27.240433 1 TAGACAG 70 0.008428767 27.11554 5 GTAGGGG 140 5.8471596E-7 27.11554 6 TCGTATC 35 0.008432551 27.11554 94-95 ATCGGAT 70 0.008428767 27.11554 8 >>END_MODULE