FastQCFastQC Report
Fri 27 May 2016
SRR939173_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939173_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731547
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79200.2899455876102443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63260.23159037717454617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.1443870451432833No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28230.10334802952319694No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114800.029.195051
GGTATCA80250.028.5133571
GTACATG106650.026.930281
TACATGG107300.026.0047912
ACATGGG104350.025.6503833
ATCAACG134850.024.6266233
TCAACGC136450.024.3378544
CAACGCA137350.024.2128725
TATCAAC140550.023.7627182
AACGCAG141550.023.5510126
CATGGGG54900.023.1259864
CGTATAC1302.996374E-421.864813
ACGCAGA161500.020.4665977
CGCAGAG163900.020.1969058
GAGTACT79900.019.94421412-13
TACCTGG20650.019.0412672
TATACCG1250.006126663618.9495032
CATGGGA49350.018.815114
AGAGTAC136650.018.2801410-11
GCAGAGT175400.018.2507139
Produced by FastQC (version 0.11.5)