##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939173_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731547 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15762459880793 33.0 31.0 34.0 30.0 34.0 2 32.25423468825541 34.0 31.0 34.0 30.0 34.0 3 32.31797256280049 34.0 31.0 34.0 30.0 34.0 4 35.74679989031856 37.0 35.0 37.0 35.0 37.0 5 35.79248828594199 37.0 35.0 37.0 35.0 37.0 6 35.87294232901722 37.0 37.0 37.0 35.0 37.0 7 35.797589058507874 37.0 36.0 37.0 35.0 37.0 8 35.79042937939563 37.0 36.0 37.0 35.0 37.0 9 37.48703024330169 39.0 38.0 39.0 35.0 39.0 10-11 37.40469100476763 39.0 38.0 39.0 35.0 39.0 12-13 37.317498106384406 39.0 37.0 39.0 34.0 39.0 14-15 38.65982609854416 40.0 38.0 41.0 34.5 41.0 16-17 38.596181028552685 40.0 38.0 41.0 34.0 41.0 18-19 38.519547714170756 40.0 38.0 41.0 34.0 41.0 20-21 38.424464964359025 40.0 38.0 41.0 34.0 41.0 22-23 38.34762499052735 40.0 38.0 41.0 34.0 41.0 24-25 38.379923903926965 40.0 38.0 41.0 34.0 41.0 26-27 38.305854155172874 40.0 38.0 41.0 34.0 41.0 28-29 38.1941972808815 40.0 38.0 41.0 34.0 41.0 30-31 38.03212904628769 40.0 38.0 41.0 33.0 41.0 32-33 37.84358423999294 40.0 38.0 41.0 33.0 41.0 34-35 37.54531424866568 40.0 37.5 41.0 32.5 41.0 36-37 37.29790957285377 40.0 37.0 41.0 31.5 41.0 38-39 37.071556154808974 40.0 37.0 41.0 31.0 41.0 40-41 36.92473074781434 40.0 37.0 41.0 31.0 41.0 42-43 36.73764665224505 40.0 36.0 41.0 30.0 41.0 44-45 36.4896066221815 40.0 36.0 41.0 30.0 41.0 46-47 36.14243375640251 39.0 35.5 41.0 29.5 41.0 48-49 36.143820699405865 40.0 36.0 41.0 29.0 41.0 50-51 35.140693350691016 38.5 34.5 40.0 27.5 40.5 52-53 35.29476922784049 38.5 35.0 40.0 27.5 40.5 54-55 35.79969409276136 39.0 35.0 41.0 28.0 41.0 56-57 35.68530525010186 39.0 35.0 41.0 28.0 41.0 58-59 35.50316066316999 39.0 35.0 41.0 28.0 41.0 60-61 35.22251621517037 39.0 35.0 41.0 27.0 41.0 62-63 34.864696269183725 38.0 35.0 40.0 26.0 41.0 64-65 34.50274368334135 37.5 34.0 40.0 26.0 41.0 66-67 34.12518565486883 37.0 34.0 40.0 25.5 41.0 68-69 33.73238608012237 36.5 34.0 39.0 25.0 41.0 70-71 33.33164338742844 36.0 34.0 39.0 24.0 41.0 72-73 32.893753978972356 35.0 33.0 38.5 22.5 40.0 74-75 32.44608384918875 35.0 33.0 37.5 21.5 39.5 76-77 32.026875429930364 35.0 32.5 37.0 20.0 39.0 78-79 31.61563447379818 35.0 32.0 36.5 19.5 39.0 80-81 31.20807824284188 35.0 32.0 36.0 18.0 37.5 82-83 30.805246990075588 35.0 31.0 35.5 14.0 37.0 84-85 30.450686186252696 35.0 31.0 35.0 8.5 37.0 86-87 30.088365860078557 34.0 31.0 35.0 4.5 36.0 88-89 29.725264291626686 34.0 30.5 35.0 2.0 36.0 90-91 29.38926018845731 34.0 30.0 35.0 2.0 36.0 92-93 29.01346782610733 34.0 30.0 35.0 2.0 35.0 94-95 28.595041930451863 34.0 29.0 35.0 2.0 35.0 96-97 28.07651378504562 34.0 29.0 35.0 2.0 35.0 98-99 27.435909761025528 34.0 28.0 35.0 2.0 35.0 100-101 24.95803422016901 31.5 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09780717895480251 1101 2 0.14522829230768508 1101 3 0.15062463978146212 1101 4 0.016627368084250804 1101 5 0.1516720228178272 1101 6 0.13850443590122552 1101 7 0.11017252735290128 1101 8 0.20415028647469313 1101 9 0.32170053164166745 1101 10-11 0.3267605017662589 1101 12-13 0.21271074812533186 1101 14-15 0.21474328294029732 1101 16-17 0.3149000548569916 1101 18-19 0.3796688907149317 1101 20-21 0.3496942452879779 1101 22-23 0.3017185671758398 1101 24-25 0.2874561011350423 1101 26-27 0.21792345470607444 1101 28-29 0.31624275246684164 1101 30-31 0.28840124430576 1101 32-33 0.2742209912319922 1101 34-35 0.14216151719303127 1101 36-37 0.13466160969059615 1101 38-39 0.11492624471559054 1101 40-41 0.05855793482488991 1101 42-43 -0.08934921536103246 1101 44-45 -0.008433136077755421 1101 46-47 -0.08882279950961447 1101 48-49 0.0999069536896755 1101 50-51 -0.027508108290945188 1101 52-53 -0.09339503824367767 1101 54-55 -0.015973178230147766 1101 56-57 0.00883980130708295 1101 58-59 0.04527235649340611 1101 60-61 0.11271266620617126 1101 62-63 0.03568836815372123 1101 64-65 -0.06426923816176355 1101 66-67 -0.11018883360510046 1101 68-69 -0.07517181508048765 1101 70-71 -0.13567654870639245 1101 72-73 -0.32791431833747 1101 74-75 -0.22272752156293762 1101 76-77 -0.35605094165116213 1101 78-79 -0.2680364774135633 1101 80-81 -0.3881474250234973 1101 82-83 -0.4528281584474172 1101 84-85 -0.49963638595375315 1101 86-87 -0.5812391706340883 1101 88-89 -0.6960919621444432 1101 90-91 -0.7438307642615882 1101 92-93 -0.7901407044595956 1101 94-95 -0.8856855116585685 1101 96-97 -0.866099010360422 1101 98-99 -0.9866519691187001 1101 100-101 -1.055693475250294 1102 1 0.11886643068097413 1102 2 0.1971016531169809 1102 3 0.22611840416620055 1102 4 0.14368761893618398 1102 5 0.2138872273709893 1102 6 0.25543248100461824 1102 7 0.2072072648147696 1102 8 0.17797943596888643 1102 9 0.28858397291627114 1102 10-11 0.1426028896768372 1102 12-13 0.131411795423233 1102 14-15 0.19541376769470986 1102 16-17 0.271129871624467 1102 18-19 0.33016800755912357 1102 20-21 0.3625923344599613 1102 22-23 0.37657092850253093 1102 24-25 0.2787311461849029 1102 26-27 0.2775150620496518 1102 28-29 0.26971471450006135 1102 30-31 0.26487986123024143 1102 32-33 0.19927139355853285 1102 34-35 0.18149049719268362 1102 36-37 0.35797826119196685 1102 38-39 0.28506130510918126 1102 40-41 0.2284695300362216 1102 42-43 0.13576898478526545 1102 44-45 0.046593715427178495 1102 46-47 0.004239966998262901 1102 48-49 0.05121875229446715 1102 50-51 0.02239956178345892 1102 52-53 0.08810998414744375 1102 54-55 0.18016814454099261 1102 56-57 0.14017488311051096 1102 58-59 -0.012250525270765422 1102 60-61 -0.03314854452015936 1102 62-63 -0.020463391913359885 1102 64-65 -0.03472589216094235 1102 66-67 -0.0035317121577946864 1102 68-69 0.021232224242247355 1102 70-71 -0.0774016463887719 1102 72-73 -0.11060892484249507 1102 74-75 -0.22131581038165393 1102 76-77 -0.3053890208318819 1102 78-79 -0.2120426149004011 1102 80-81 -0.24907268684797046 1102 82-83 -0.5497821629791702 1102 84-85 -0.49936162636839043 1102 86-87 -0.603339475724276 1102 88-89 -0.4879084976758534 1102 90-91 -0.5946581652856935 1102 92-93 -0.7085330932622504 1102 94-95 -0.9431004521570578 1102 96-97 -0.9090070015823883 1102 98-99 -1.0763899662283691 1102 100-101 -0.9476964547860582 1103 1 0.14052873798799936 1103 2 0.2173636320807475 1103 3 0.19322703569828548 1103 4 0.19633940812868644 1103 5 0.20449327111298743 1103 6 0.21021989265802432 1103 7 0.21809476551040774 1103 8 0.2580826693152076 1103 9 0.305643213831182 1103 10-11 0.29018761893278366 1103 12-13 0.33372098068673495 1103 14-15 0.33037641169271126 1103 16-17 0.3218279488686093 1103 18-19 0.37123282752683906 1103 20-21 0.36313041393015055 1103 22-23 0.31585872971572826 1103 24-25 0.37154237663735046 1103 26-27 0.3575842089136998 1103 28-29 0.28250201485067095 1103 30-31 0.31016723114508693 1103 32-33 0.26678419338044534 1103 34-35 0.2794178303776107 1103 36-37 0.12377769354769441 1103 38-39 0.2603684312708978 1103 40-41 0.23923285854643694 1103 42-43 0.3506286041296889 1103 44-45 0.2010833920925421 1103 46-47 0.23831879720584226 1103 48-49 0.26650375702484297 1103 50-51 0.2939752615998046 1103 52-53 0.20258839140438312 1103 54-55 0.22504995488102253 1103 56-57 0.19647601283391936 1103 58-59 0.182330767511246 1103 60-61 0.2027187825431227 1103 62-63 0.16309544552890287 1103 64-65 0.03807518191106851 1103 66-67 -0.026389481093183065 1103 68-69 -0.22680362917196106 1103 70-71 -0.1749505500562023 1103 72-73 -0.13021734768182824 1103 74-75 -0.21267036544870876 1103 76-77 -0.16099137766865113 1103 78-79 -0.12782639529998008 1103 80-81 -0.23622903945524243 1103 82-83 -0.20377654218780705 1103 84-85 -0.2484461608444306 1103 86-87 -0.2470839105843119 1103 88-89 -0.3257347540062021 1103 90-91 -0.42177152718674193 1103 92-93 -0.41795870125379153 1103 94-95 -0.5670928140024607 1103 96-97 -0.6553023574730901 1103 98-99 -0.8375404197872776 1103 100-101 -0.9625807694390716 1104 1 0.18738712646433697 1104 2 0.23024429958114467 1104 3 0.29936170067607293 1104 4 0.21052305509204672 1104 5 0.18970709029391486 1104 6 0.22004219872915343 1104 7 0.24853460853025666 1104 8 0.294585843999144 1104 9 0.3389839226252249 1104 10-11 0.3491167689851409 1104 12-13 0.38839214114016585 1104 14-15 0.39485648857603195 1104 16-17 0.37182009919143155 1104 18-19 0.3889559424662039 1104 20-21 0.3477419503111463 1104 22-23 0.38869854542070925 1104 24-25 0.41933937896447304 1104 26-27 0.35646299383053304 1104 28-29 0.44044923556283067 1104 30-31 0.37619347685036075 1104 32-33 0.2583237811937238 1104 34-35 0.2299763290507002 1104 36-37 0.20859203278228478 1104 38-39 0.39522111509059243 1104 40-41 0.3074940027448392 1104 42-43 0.3666668837750322 1104 44-45 0.4355497345513655 1104 46-47 0.23109224045920485 1104 48-49 0.37252168106808625 1104 50-51 0.4009523860672317 1104 52-53 0.4756445167635661 1104 54-55 0.49535442528054574 1104 56-57 0.37041916845100786 1104 58-59 0.4094660303365103 1104 60-61 0.4240565817681272 1104 62-63 0.40686248384737667 1104 64-65 0.5087101923415958 1104 66-67 0.46792820622120246 1104 68-69 0.3601797482579272 1104 70-71 0.46005209774535416 1104 72-73 0.33857248650085126 1104 74-75 0.39824239300484265 1104 76-77 0.36509574404050227 1104 78-79 0.3243827113967157 1104 80-81 0.33021487937140037 1104 82-83 0.25581619848430037 1104 84-85 0.2723921378141938 1104 86-87 0.24379682319480622 1104 88-89 0.25247648365190045 1104 90-91 0.18435577348836318 1104 92-93 0.1269184387456157 1104 94-95 -0.06124413502446302 1104 96-97 -0.1588734584765099 1104 98-99 -0.42019049088627014 1104 100-101 -0.6504790275806442 1105 1 0.23957707714428267 1105 2 0.22396594213967091 1105 3 0.2710168359798715 1105 4 0.2382750602119117 1105 5 0.17763036242028107 1105 6 0.2659598415551372 1105 7 0.25441496679319897 1105 8 0.2548900427312759 1105 9 0.2941222512197186 1105 10-11 0.3044833899084267 1105 12-13 0.3365150178145484 1105 14-15 0.38788051817443403 1105 16-17 0.31522270560845556 1105 18-19 0.35151552294323096 1105 20-21 0.35274981477491707 1105 22-23 0.34171848358755597 1105 24-25 0.25833645287450935 1105 26-27 0.3433673084600244 1105 28-29 0.3770097585019201 1105 30-31 0.42385061488603526 1105 32-33 0.33161771791504435 1105 34-35 0.3238000981984328 1105 36-37 0.3420052578173909 1105 38-39 0.5744611595391405 1105 40-41 0.45677959421937686 1105 42-43 0.3880451708502406 1105 44-45 0.4035876941210148 1105 46-47 0.4717761940875249 1105 48-49 0.46246734622022956 1105 50-51 0.4306705867288656 1105 52-53 0.5277728571706675 1105 54-55 0.6199484661184513 1105 56-57 0.5592230535597906 1105 58-59 0.602046768449874 1105 60-61 0.6340088696266051 1105 62-63 0.6107101011058873 1105 64-65 0.5307376160769977 1105 66-67 0.45939589983188256 1105 68-69 0.43607540560928726 1105 70-71 0.4286295850014241 1105 72-73 0.48436229199069913 1105 74-75 0.6754469104547169 1105 76-77 0.5913339990847533 1105 78-79 0.5167918322642926 1105 80-81 0.39448418471955193 1105 82-83 0.3075443109174749 1105 84-85 0.3372293879901136 1105 86-87 0.3822920997405568 1105 88-89 0.427225823769259 1105 90-91 0.29728254800749454 1105 92-93 0.2709117555434126 1105 94-95 0.12364837335739765 1105 96-97 0.09094998908789265 1105 98-99 0.10147180840274217 1105 100-101 0.16755200680330518 1106 1 0.19870971934778936 1106 2 0.21546413324416136 1106 3 0.208596260173465 1106 4 0.2474350638601024 1106 5 0.3003510020700588 1106 6 0.2587029783927619 1106 7 0.2975320433748436 1106 8 0.32588995152646305 1106 9 0.25502730700666376 1106 10-11 0.283450039347521 1106 12-13 0.3088419566769218 1106 14-15 0.3077008446876732 1106 16-17 0.38033382347548184 1106 18-19 0.35297632007331714 1106 20-21 0.37967727654490346 1106 22-23 0.47793363574758985 1106 24-25 0.5112033242453222 1106 26-27 0.523052195822082 1106 28-29 0.46619438745032227 1106 30-31 0.44847059482097507 1106 32-33 0.42809265179155176 1106 34-35 0.366512073390723 1106 36-37 0.444947373161078 1106 38-39 0.46939017179098386 1106 40-41 0.42429623909822567 1106 42-43 0.357617724281063 1106 44-45 0.3309339380026941 1106 46-47 0.48700739829510553 1106 48-49 0.45092917700489465 1106 50-51 0.3264820055717834 1106 52-53 0.34279429030234354 1106 54-55 0.3749046878069535 1106 56-57 0.4641017511550203 1106 58-59 0.46646852991887755 1106 60-61 0.47518100351527437 1106 62-63 0.4751759449024462 1106 64-65 0.5052071080661662 1106 66-67 0.46584712045633836 1106 68-69 0.4141915701427692 1106 70-71 0.46132195115836083 1106 72-73 0.37712670995195907 1106 74-75 0.32363649486477186 1106 76-77 0.46113380451447483 1106 78-79 0.44933599059584495 1106 80-81 0.5955900431088743 1106 82-83 0.7005869035663679 1106 84-85 0.765014935066997 1106 86-87 0.69797780794595 1106 88-89 0.7033985656900335 1106 90-91 0.6192925501356115 1106 92-93 0.5594830321068152 1106 94-95 0.5050144694872749 1106 96-97 0.5399482561963964 1106 98-99 0.5748080805579114 1106 100-101 0.571182718504879 1107 1 0.06860507002539151 1107 2 0.13274862345080862 1107 3 0.05928576663582419 1107 4 0.1285887474959253 1107 5 0.07684607017851164 1107 6 0.10290878630673461 1107 7 0.14387802939739913 1107 8 0.18591585611799388 1107 9 0.20318240348819927 1107 10-11 0.220311227619014 1107 12-13 0.21736034182185904 1107 14-15 0.2189811036174234 1107 16-17 0.30836973560315073 1107 18-19 0.3181889511753795 1107 20-21 0.21378639237646269 1107 22-23 0.34008156780902254 1107 24-25 0.26206039740400655 1107 26-27 0.23528667012876525 1107 28-29 0.14078632479141362 1107 30-31 0.29452849390632707 1107 32-33 0.3858489279193975 1107 34-35 0.24324919841667736 1107 36-37 0.2570770093787189 1107 38-39 0.32484153461354026 1107 40-41 0.17987327694303445 1107 42-43 0.1408579327375179 1107 44-45 0.16305280000121058 1107 46-47 0.2782215453385106 1107 48-49 0.3181410646567997 1107 50-51 0.2288619173161095 1107 52-53 0.3704035898183733 1107 54-55 0.43547728963566357 1107 56-57 0.3064713678570925 1107 58-59 0.3347653175462355 1107 60-61 0.23631547651510942 1107 62-63 0.27187608618701375 1107 64-65 0.10226256625363561 1107 66-67 -0.008134519809622986 1107 68-69 0.20632123540143255 1107 70-71 0.08181961263007054 1107 72-73 0.09867159067322717 1107 74-75 0.14179148970273303 1107 76-77 0.21203349537279337 1107 78-79 0.24818284421893466 1107 80-81 0.26643481739479213 1107 82-83 0.2635815992237198 1107 84-85 0.33314727790787657 1107 86-87 0.20764367646853188 1107 88-89 0.25482041412203316 1107 90-91 0.34122164357655294 1107 92-93 0.41226980856790263 1107 94-95 0.43297777906021295 1107 96-97 0.35434040582735804 1107 98-99 0.37315644504016277 1107 100-101 0.3509331399627129 1108 1 -0.12004125583228387 1108 2 -0.043510785833618115 1108 3 -0.041487286162833925 1108 4 -0.07656812480142605 1108 5 -0.06921713854877964 1108 6 0.056417298149703754 1108 7 0.1507744639207118 1108 8 0.15888664087876947 1108 9 0.19106145178437828 1108 10-11 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-1.060097029252887 2316 98-99 -0.9814208491288774 2316 100-101 -0.7413913176138998 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8268.0 3 2118.0 4 2046.0 5 2336.0 6 2738.0 7 3222.0 8 3868.0 9 4779.0 10 5937.0 11 7183.0 12 9503.0 13 15044.0 14 13238.0 15 13016.0 16 15591.0 17 20773.0 18 19927.0 19 13580.0 20 13490.0 21 14501.0 22 15046.0 23 16348.0 24 18734.0 25 21215.0 26 24242.0 27 28451.0 28 33330.0 29 40320.0 30 48895.0 31 60603.0 32 76635.0 33 100275.0 34 142400.0 35 213381.0 36 355031.0 37 595335.0 38 627918.0 39 122184.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.021017871802734 18.37227198345605 13.75384994003552 23.8528602047057 2 15.710449809335733 19.814569652933855 39.88053611772071 24.594444420009705 3 19.672622014507457 23.71982255633807 29.233491464995044 27.37406396415943 4 13.10824976542341 15.903449741965753 36.318796915317854 34.669503577292986 5 14.24732197812712 36.84197792183083 33.707828097775064 15.202872002266984 6 33.103122050452285 34.875268940126745 17.20996969110724 14.811639318313723 7 29.24218561630132 32.18237702665366 20.328500560277256 18.24693679676776 8 26.65470324196106 34.675780074778125 19.601535139231473 19.067981544029347 9 26.57936064171797 16.929481227653493 20.275695137063448 36.21546299356508 10-11 25.543357685308628 26.07178889279519 26.93786353232922 21.446989889566964 12-13 26.161124121093355 23.356830692598038 27.62319899483383 22.858846191474775 14-15 22.83720040585299 24.935280079235874 25.845004904606593 26.382514610304547 16-17 22.752180367823033 28.155304767864653 26.461498609928498 22.631016254383816 18-19 22.495173521454078 26.734980935020403 28.70676025799605 22.063085285529468 20-21 23.090011954489384 26.056444100118664 28.65616073987368 22.197383205518268 22-23 22.702533614956057 25.967161852068905 28.620459135934272 22.709845397040763 24-25 22.70256400387455 26.15709163021674 28.700568078202167 22.439776287706543 26-27 22.503524449009305 26.380674949396756 28.59673412055192 22.519066481042017 28-29 22.221715176299867 26.676028078370486 28.781081151416988 22.321175593912663 30-31 22.462093905155143 26.227818094724647 28.605353307050457 22.704734693069756 32-33 22.21490493914775 26.46176319525107 28.647566358992627 22.67576550660855 34-35 22.892519911202136 26.53871977691814 28.258517263721135 22.31024304815859 36-37 22.870479443336276 26.22706473489383 28.351581969498866 22.55087385227103 38-39 22.428206447071258 26.52041275879488 28.497352537740568 22.55402825639329 40-41 22.728376692131178 26.34072019257693 28.460805729211575 22.47009738608031 42-43 22.614879722330326 26.441684144858467 28.087809798037682 22.855626334773522 44-45 22.61485554781619 26.628502418035733 28.103050828503484 22.65359120564459 46-47 22.764815816290866 26.750067139772128 27.76290279264663 22.72221425129037 48-49 22.85850104685468 26.822125951123727 27.637552253050927 22.68182074897067 50-51 22.676264762498633 26.982127271339344 27.652761567885193 22.68884639827683 52-53 23.000534008898434 26.919383051407415 27.41912193338078 22.66096100631337 54-55 22.47375554367412 26.715152491616323 27.69165575542486 23.1194362092847 56-57 22.67814757851983 27.128848944622565 27.442553147670463 22.750450329187142 58-59 22.856186333003453 26.71987413171863 27.55354191144987 22.87039762382805 60-61 22.733044765927044 26.72156357126239 27.96476547129389 22.580626191516675 62-63 22.570445173183387 26.77525293763691 27.9234581360074 22.730843753172294 64-65 22.304524084194284 26.730281417179807 27.849110486831098 23.116084011794808 66-67 22.421841467298346 26.948947641772296 27.50814604617568 23.12106484475368 68-69 22.42871345189569 26.754841692885428 27.804665593689172 23.011779261529707 70-71 22.41379532397761 26.781768756295794 27.663672282861178 23.14076363686542 72-73 22.55200985051842 26.722493139278757 27.709697436536146 23.01579957366668 74-75 22.379679129627018 27.064811208403473 27.41423209369749 23.141277568272027 76-77 22.412021886670427 26.825463423409435 27.507182402960993 23.255332286959142 78-79 22.49831638933832 26.872357117452577 27.51908061433123 23.110245878877866 80-81 22.477053000392267 27.070462014856076 27.47600567084354 22.97647931390811 82-83 22.58976151575196 26.94694879176086 27.46857158307183 22.994718109415345 84-85 22.579733234551753 26.82212381258402 27.32989375768704 23.268249195177187 86-87 22.542990153971797 26.742552021351145 27.587695499667998 23.12676232500906 88-89 22.488660830047845 26.765444744173315 27.416766371892397 23.329128053886443 90-91 22.467517175144152 26.77190083861068 27.576161231090783 23.184420755154385 92-93 22.60726786034596 26.541626230895538 27.615532855309695 23.235573053448807 94-95 22.469528478630792 26.400372673481478 27.62802785704837 23.502070990839364 96-97 22.510482421361143 26.29655455366687 27.717361907092354 23.475601117879634 98-99 22.383409947327763 26.329623961332604 27.457631421653662 23.82933466968597 100-101 22.54570190297903 25.813433262740897 27.984311593156537 23.656553241123536 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2563.0 1 1475.0 2 417.5 3 471.0 4 447.5 5 450.5 6 708.5 7 1357.0 8 2135.5 9 2580.5 10 2566.0 11 2597.0 12 2893.0 13 3185.0 14 3480.5 15 3764.0 16 3969.5 17 4137.0 18 4297.0 19 4540.0 20 4832.0 21 5338.5 22 6174.0 23 7456.0 24 9187.5 25 11210.0 26 14163.0 27 17654.5 28 20963.5 29 24319.5 30 28407.0 31 34032.0 32 40125.0 33 44239.0 34 47630.5 35 51425.5 36 56880.5 37 63146.5 38 67434.0 39 70006.0 40 72504.5 41 77967.0 42 84265.0 43 89706.5 44 94025.5 45 98396.0 46 100432.5 47 102758.0 48 112247.5 49 114488.5 50 108849.0 51 105152.0 52 101600.5 53 99989.5 54 97936.5 55 90767.0 56 80332.0 57 71701.5 58 64453.5 59 55927.5 60 47564.5 61 40182.0 62 33887.0 63 27125.5 64 20750.0 65 16141.5 66 12122.5 67 10024.0 68 7586.5 69 4895.0 70 3679.0 71 2893.0 72 2398.0 73 1909.5 74 1471.5 75 1286.0 76 964.5 77 589.5 78 351.0 79 230.0 80 159.0 81 105.0 82 78.0 83 62.0 84 46.0 85 32.5 86 27.5 87 22.5 88 17.5 89 11.0 90 9.5 91 9.0 92 8.0 93 6.5 94 5.0 95 5.0 96 4.5 97 3.5 98 3.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3332177700035914 2 0.1168202487454911 3 0.11052345063072316 4 0.11813818323462859 5 0.1352347223020508 6 0.08475050950981257 7 0.09302420935828672 8 0.09789324510982238 9 0.08687384840897851 10-11 0.08553760927415856 12-13 0.08954632667861838 14-15 0.09079104258502599 16-17 0.08021095738056126 18-19 0.09483636928085074 20-21 0.10488561976052398 22-23 0.11253696165579431 24-25 0.12774812221792267 26-27 0.12670475741402215 28-29 0.12176250307975663 30-31 0.11085293425300755 32-33 0.1042449571616377 34-35 0.3279826413384064 36-37 0.48884386759590814 38-39 0.46210078025382684 40-41 0.43969589393848985 42-43 0.47302865372625846 44-45 0.48036881664492687 46-47 0.4882581189340692 48-49 0.4994788667374202 50-51 0.4868852705078843 52-53 0.49157125980259536 54-55 0.47667127821706895 56-57 0.45487044520925324 58-59 0.43486346747831905 60-61 0.39354988217299575 62-63 0.46475495387778426 64-65 0.4968429977591453 66-67 0.505848883434918 68-69 0.5193577119485772 70-71 0.5110474028087381 72-73 0.5022977821725197 74-75 0.5091437196577617 76-77 0.48586021034966637 78-79 0.46449868883822976 80-81 0.4659447558471445 82-83 0.4798562865658178 84-85 0.47927053790397894 86-87 0.48294977168615444 88-89 0.4955067586243253 90-91 0.5109558795803257 92-93 0.5037255445357521 94-95 0.5083749245390982 96-97 0.5089423685552547 98-99 0.5016022056365862 100-101 0.48915504657250997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.40987840985585 #Duplication Level Percentage of deduplicated Percentage of total 1 75.06219270883244 40.09062585755909 2 13.955724389398425 14.907470855184593 3 4.729178387076996 7.5775452789690165 4 2.119801825316738 4.528734309726298 5 1.1482519954606876 3.0663999730714835 6 0.7002057510640851 2.243874241572875 7 0.4456234950407859 1.6660487680692366 8 0.3000619662715813 1.2821018507189939 9 0.22770793935839084 1.0945668018681438 >10 1.1547537266754582 11.08982168412946 >50 0.0909077270322969 3.3658903626266703 >100 0.05961611409637219 5.92094626402867 >500 0.0045319805609588485 1.5694539393085107 >1k 0.00130466107054766 1.0740530930904497 >5k 1.3733274426817475E-4 0.5224667200767106 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7920 0.2899455876102443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6326 0.23159037717454617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3944 0.1443870451432833 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2823 0.10334802952319694 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.660929136492984E-5 0.0 0.0 0.0 0.0 7 7.321858272985968E-5 0.0 0.0 0.0 0.0 8 7.321858272985968E-5 0.0 0.0 0.0 0.0 9 7.321858272985968E-5 0.0 0.0 0.0 0.0 10-11 1.0982787409478951E-4 0.0 0.0 3.660929136492984E-5 0.0 12-13 1.0982787409478951E-4 0.0 0.0 7.321858272985968E-5 0.0 14-15 2.5626503955450885E-4 0.0 0.0 7.321858272985968E-5 0.0 16-17 2.745696852369738E-4 0.0 0.0 7.321858272985968E-5 0.0 18-19 2.928743309194387E-4 0.0 0.0 1.0982787409478951E-4 0.0 20-21 2.928743309194387E-4 0.0 0.0 1.6474181114218428E-4 0.0 22-23 2.928743309194387E-4 0.0 0.0 2.5626503955450885E-4 0.0 24-25 2.928743309194387E-4 0.0 0.0 3.2948362228436856E-4 0.0 26-27 2.928743309194387E-4 0.0 0.0 8.054044100284564E-4 0.0 28-29 2.928743309194387E-4 0.0 0.0 0.0027273922066872727 0.0 30-31 2.928743309194387E-4 0.0 0.0 0.008035739454602099 0.0 32-33 2.928743309194387E-4 0.0 0.0 0.017737201666308506 0.0 34-35 2.928743309194387E-4 0.0 0.0 0.03567575443512412 0.0 36-37 2.928743309194387E-4 0.0 0.0 0.0690268188685752 0.0 38-39 3.2948362228436856E-4 0.0 0.0 0.11665550693434892 0.0 40-41 3.2948362228436856E-4 0.0 0.0 0.16862239602686685 0.0 42-43 3.477882679668334E-4 0.0 0.0 0.22166925921465017 0.0 44-45 4.027022050142282E-4 0.0 0.0 0.28965271327932485 0.0 46-47 4.7592078774408785E-4 0.0 0.0 0.3737442555445687 0.0 48-49 5.125300791090177E-4 0.0 0.0 0.46438886096413495 0.0 50-51 5.125300791090177E-4 0.0 0.0 0.5663091281240996 0.0 52-53 5.125300791090177E-4 0.0 0.0 0.6723845498539838 0.0 54-55 5.125300791090177E-4 0.0 0.0 0.7908522167108968 0.0 56-57 5.125300791090177E-4 0.0 0.0 0.9304800539767392 0.0 58-59 5.125300791090177E-4 0.0 0.0 1.0898402992882787 0.0 60-61 5.125300791090177E-4 0.0 0.0 1.2603114645290745 0.0 62-63 5.125300791090177E-4 0.0 0.0 1.4380312694601265 0.0 64-65 5.125300791090177E-4 0.0 0.0 1.6268986036117994 0.0 66-67 5.857486618388774E-4 0.0 0.0 1.8466092657384259 0.0 68-69 5.857486618388774E-4 0.0 0.0 2.1026363448990626 0.0 70-71 5.857486618388774E-4 0.0 0.0 2.383338086439662 0.0 72-73 5.857486618388774E-4 0.0 0.0 2.6677373664081196 0.0 74-75 6.040533075213424E-4 0.0 0.0 2.9652244680395397 0.0 76-77 6.223579532038073E-4 0.0 0.0 3.295861283001903 0.0 78-79 6.223579532038073E-4 0.0 0.0 3.6606728714534293 0.0 80-81 8.237090557109213E-4 0.0 0.0 4.077323216477696 0.0 82-83 8.420137013933863E-4 0.0 0.0 4.50658546237718 0.0 84-85 8.96927638440781E-4 0.0 0.0 4.938373749380846 0.0 86-87 9.518415754881757E-4 0.0 0.0 5.390498497737729 0.0 88-89 9.518415754881757E-4 0.0 0.0 5.886920488646178 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11480 0.0 29.19505 1 GGTATCA 8025 0.0 28.513357 1 GTACATG 10665 0.0 26.93028 1 TACATGG 10730 0.0 26.004791 2 ACATGGG 10435 0.0 25.650383 3 ATCAACG 13485 0.0 24.626623 3 TCAACGC 13645 0.0 24.337854 4 CAACGCA 13735 0.0 24.212872 5 TATCAAC 14055 0.0 23.762718 2 AACGCAG 14155 0.0 23.551012 6 CATGGGG 5490 0.0 23.125986 4 CGTATAC 130 2.996374E-4 21.86481 3 ACGCAGA 16150 0.0 20.466597 7 CGCAGAG 16390 0.0 20.196905 8 GAGTACT 7990 0.0 19.944214 12-13 TACCTGG 2065 0.0 19.041267 2 TATACCG 125 0.0061266636 18.949503 2 CATGGGA 4935 0.0 18.81511 4 AGAGTAC 13665 0.0 18.28014 10-11 GCAGAGT 17540 0.0 18.250713 9 >>END_MODULE