FastQCFastQC Report
Fri 27 May 2016
SRR939173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731547
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79830.2922519729662349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67680.24777168395784516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.15288040073994702No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32320.11832122969145323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.028.0147341
GTATCAA130800.027.3690911
GTACATG103600.026.3756031
TACATGG104950.025.091552
ACATGGG102500.024.3951633
ATCAACG152350.023.2645743
TCAACGC153850.023.068594
CAACGCA154900.022.9122165
TATCAAC158250.022.4871542
AACGCAG158250.022.3972056
GTGTAGC14750.021.3011071
GAGTACT89050.021.1808812-13
CATGGGG54450.020.5651114
ACGCAGA176650.020.0105767
CGCAGAG179250.019.6938538
GTACTTT94750.019.68131414-15
AGAGTAC144000.018.99667510-11
AGTACTT95800.018.47498512-13
CATGGGA47300.018.4575314
GCAGAGT188950.018.3825179
Produced by FastQC (version 0.11.5)