##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939173_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731547 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53255645976437 34.0 31.0 34.0 31.0 34.0 2 32.73771968778132 34.0 31.0 34.0 31.0 34.0 3 32.82736339517497 34.0 31.0 34.0 31.0 34.0 4 36.27773199582508 37.0 37.0 37.0 35.0 37.0 5 36.23822141811947 37.0 37.0 37.0 35.0 37.0 6 36.27317596951471 37.0 37.0 37.0 35.0 37.0 7 36.22625750170142 37.0 37.0 37.0 35.0 37.0 8 36.21617676723117 37.0 37.0 37.0 35.0 37.0 9 37.96690520060611 39.0 38.0 39.0 35.0 39.0 10-11 37.91237566111804 39.0 38.0 39.0 35.0 39.0 12-13 37.844425338462045 39.0 38.0 39.0 35.0 39.0 14-15 39.2337964530722 41.0 39.0 41.0 36.0 41.0 16-17 39.179633921730066 40.0 39.0 41.0 36.0 41.0 18-19 39.19716867401513 40.5 38.5 41.0 36.0 41.0 20-21 39.2594753083143 41.0 39.0 41.0 36.0 41.0 22-23 39.219484416705995 41.0 39.0 41.0 36.0 41.0 24-25 39.16582782577052 41.0 39.0 41.0 36.0 41.0 26-27 39.04113786070677 40.5 39.0 41.0 35.0 41.0 28-29 38.89482754644163 40.0 38.0 41.0 35.0 41.0 30-31 38.71412371817142 40.0 38.0 41.0 35.0 41.0 32-33 38.587761806771034 40.0 38.0 41.0 34.5 41.0 34-35 38.547216284398544 40.0 38.0 41.0 34.5 41.0 36-37 38.44634487343619 40.0 38.0 41.0 34.0 41.0 38-39 38.320134707548505 40.0 38.0 41.0 34.0 41.0 40-41 38.159486364320294 40.0 38.0 41.0 34.0 41.0 42-43 38.00327946032047 40.0 38.0 41.0 33.0 41.0 44-45 37.79577287156326 40.0 37.5 41.0 33.0 41.0 46-47 37.516149639746274 40.0 37.0 41.0 33.0 41.0 48-49 37.21671748646463 40.0 37.0 41.0 32.0 41.0 50-51 36.97055990616306 40.0 36.5 41.0 31.0 41.0 52-53 36.73825583085336 40.0 36.0 41.0 31.0 41.0 54-55 36.49327157834004 39.0 35.5 41.0 31.0 41.0 56-57 36.22881685726074 39.0 35.0 41.0 30.0 41.0 58-59 35.93127484169227 39.0 35.0 40.0 29.5 41.0 60-61 35.57958786724153 38.0 35.0 40.0 29.0 41.0 62-63 35.20194783395637 38.0 34.5 40.0 28.0 41.0 64-65 34.80389812073525 37.0 34.0 40.0 28.0 41.0 66-67 34.451098772966375 36.5 34.0 39.0 27.5 41.0 68-69 33.99534201681318 36.0 34.0 39.0 26.5 40.5 70-71 33.567587524578556 35.5 33.0 39.0 26.0 40.0 72-73 33.10922766476286 35.0 33.0 38.0 26.0 40.0 74-75 32.62775123400769 35.0 32.5 37.0 25.0 39.0 76-77 30.645408078279452 33.5 30.0 35.5 21.5 37.5 78-79 31.62313828024925 34.5 31.5 36.0 22.5 38.5 80-81 31.658265993592643 35.0 32.0 36.0 23.0 37.5 82-83 31.37709748358714 35.0 32.0 35.5 22.5 37.0 84-85 31.01883108729229 34.5 31.5 35.0 20.5 36.5 86-87 30.484241347485508 34.0 31.0 35.0 18.5 36.0 88-89 29.965577930747667 34.0 30.5 35.0 12.0 36.0 90-91 29.540577189409518 34.0 30.0 35.0 7.0 35.0 92-93 29.284384453205455 34.0 30.0 35.0 2.0 35.0 94-95 28.971361649643956 34.0 29.5 35.0 2.0 35.0 96-97 28.45094446480328 34.0 29.0 35.0 2.0 35.0 98-99 27.72449751001905 34.0 29.0 35.0 2.0 35.0 100-101 24.13174073153418 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12130388133288506 1101 2 0.12161255142915195 1101 3 0.1364513631863744 1101 4 0.11885890792099474 1101 5 0.06612212226789183 1101 6 0.10035964393659924 1101 7 0.13533491208164605 1101 8 0.14738033726909094 1101 9 0.1521404773177224 1101 10-11 0.2546775917871429 1101 12-13 0.22678017270467876 1101 14-15 0.31477501537219865 1101 16-17 0.25975591549217114 1101 18-19 0.21282552463528503 1101 20-21 0.21161122171762514 1101 22-23 0.22953283217150044 1101 24-25 0.05580632336940283 1101 26-27 0.1347712207001095 1101 28-29 0.10780673516192962 1101 30-31 0.15405118275067053 1101 32-33 0.1697087787060667 1101 34-35 0.19874207448054904 1101 36-37 0.2096062048301377 1101 38-39 0.2831305555880377 1101 40-41 0.26073459551158606 1101 42-43 0.3579256979638288 1101 44-45 0.301867896706959 1101 46-47 0.2700842231320735 1101 48-49 0.2736388965004011 1101 50-51 0.30280143039375673 1101 52-53 0.29662890732358704 1101 54-55 0.25319618646373243 1101 56-57 0.2624066644252565 1101 58-59 0.4355970102423612 1101 60-61 0.4218271373206619 1101 62-63 0.2626212192786994 1101 64-65 0.21579773696404203 1101 66-67 0.35802105538677864 1101 68-69 0.28196944761121046 1101 70-71 0.2305832649736672 1101 72-73 0.17083498517455808 1101 74-75 0.2947169519441175 1101 76-77 0.27274440307169456 1101 78-79 0.386659883088214 1101 80-81 0.30143086390823015 1101 82-83 0.462537855202541 1101 84-85 0.4011760501574635 1101 86-87 0.5039530320318626 1101 88-89 0.6838540071875236 1101 90-91 0.66996938989573 1101 92-93 0.5422962707857479 1101 94-95 0.36127938012072747 1101 96-97 0.4999919875614225 1101 98-99 0.43787467930121693 1101 100-101 0.032834971272372115 1102 1 0.1025051516499289 1102 2 0.12837716810385302 1102 3 0.14002234571951533 1102 4 0.0561735790123592 1102 5 0.008986401737637095 1102 6 0.09669410128641687 1102 7 0.10196678776185308 1102 8 0.07026120936315294 1102 9 0.14611360797125883 1102 10-11 0.15713347860047122 1102 12-13 0.13758589626799278 1102 14-15 0.16801503749569946 1102 16-17 0.2300707293157913 1102 18-19 0.16433268801019096 1102 20-21 0.22438620432212275 1102 22-23 0.21233002748274288 1102 24-25 0.18396070614162596 1102 26-27 0.29422723240414683 1102 28-29 0.11292770074561531 1102 30-31 0.14062141593949207 1102 32-33 0.25784549836208726 1102 34-35 0.3139836488172918 1102 36-37 0.3943276093154964 1102 38-39 0.2989702672688921 1102 40-41 0.3314669011727105 1102 42-43 0.25661122312930473 1102 44-45 0.3262626227502423 1102 46-47 0.33529323667379174 1102 48-49 0.3392836894494309 1102 50-51 0.26163388057128145 1102 52-53 0.3794167358307803 1102 54-55 0.25893714526764455 1102 56-57 0.2263846122922999 1102 58-59 0.18503143369326835 1102 60-61 0.2536845299592443 1102 62-63 0.26979529910170896 1102 64-65 0.15053207655974887 1102 66-67 0.17696073316871974 1102 68-69 0.08739148903924843 1102 70-71 0.1327831258097163 1102 72-73 0.099279292833927 1102 74-75 0.14970410604142614 1102 76-77 0.30264680989429493 1102 78-79 0.28117628837644304 1102 80-81 0.29997811720445355 1102 82-83 0.15074549494633516 1102 84-85 0.08135321656542871 1102 86-87 0.0483820138156581 1102 88-89 0.2232779933424922 1102 90-91 0.30240618627032845 1102 92-93 0.23206638265215318 1102 94-95 0.1440270651820157 1102 96-97 0.10327036897768238 1102 98-99 0.18047044372163512 1102 100-101 -0.1785158110787819 1103 1 0.006300577104880745 1103 2 0.010918628396574093 1103 3 0.09183936391752212 1103 4 0.0663480461502104 1103 5 0.07958298474169823 1103 6 0.03424766575856353 1103 7 0.08567623180437067 1103 8 0.09800691029253272 1103 9 0.056048078448192484 1103 10-11 0.11131742038911341 1103 12-13 0.10630459235569134 1103 14-15 0.14181783676109916 1103 16-17 0.10022453587152569 1103 18-19 0.11943661699900332 1103 20-21 0.18047168487196785 1103 22-23 0.15642019666299944 1103 24-25 0.13191482156262424 1103 26-27 0.16863419717681438 1103 28-29 0.14072000972218746 1103 30-31 0.10363340881611549 1103 32-33 0.08582945684614174 1103 34-35 0.0899220705436008 1103 36-37 0.13587033567756635 1103 38-39 0.13761912085006145 1103 40-41 0.16751300835729666 1103 42-43 0.08454266060495996 1103 44-45 0.07456038556397715 1103 46-47 0.0070303255490742345 1103 48-49 0.11422351637357053 1103 50-51 0.0239321442476097 1103 52-53 -0.010107236009609721 1103 54-55 0.06396559704570137 1103 56-57 0.012908625871240531 1103 58-59 0.08273094396094649 1103 60-61 0.039725419825124675 1103 62-63 0.03359858531685944 1103 64-65 -0.06990207392163228 1103 66-67 -0.13703420255391308 1103 68-69 -0.10090849983047434 1103 70-71 -0.07950157283794823 1103 72-73 -0.13319573360048764 1103 74-75 -0.22645796474715496 1103 76-77 -0.15157295748285549 1103 78-79 -0.09825912817428417 1103 80-81 -0.03668850308604732 1103 82-83 0.04651111907678285 1103 84-85 -0.11818452899637322 1103 86-87 0.031332164074438396 1103 88-89 0.025163184505050396 1103 90-91 0.1006924709828887 1103 92-93 0.09887449662621961 1103 94-95 -0.2232022603230206 1103 96-97 -0.3322769625377049 1103 98-99 -0.4067022564842766 1103 100-101 -0.544133680743208 1104 1 0.08652126517176839 1104 2 0.10164934798267922 1104 3 0.1353818077412825 1104 4 0.05533205356271509 1104 5 0.10547520348558947 1104 6 0.13113310131020484 1104 7 0.12181119135426144 1104 8 0.14291392592895846 1104 9 0.18659925633492946 1104 10-11 0.141870656933186 1104 12-13 0.05926184525655742 1104 14-15 0.023653750724626832 1104 16-17 0.08628583565755577 1104 18-19 0.08888329676967288 1104 20-21 0.10487812207588121 1104 22-23 0.11130823583037852 1104 24-25 0.1021933327951885 1104 26-27 0.08561030403150482 1104 28-29 0.037716512347898856 1104 30-31 0.1550521417275803 1104 32-33 0.0918085174556893 1104 34-35 0.17337116848346312 1104 36-37 0.17843207196590072 1104 38-39 0.21010871375140283 1104 40-41 0.26673476893444814 1104 42-43 0.19518963467993444 1104 44-45 0.30555596564339993 1104 46-47 0.35972109111520467 1104 48-49 0.206007414928294 1104 50-51 0.20684518790052664 1104 52-53 0.12642484356235428 1104 54-55 0.21022474886549247 1104 56-57 0.24159901184103916 1104 58-59 0.20188352026182343 1104 60-61 0.26788181016530643 1104 62-63 0.2108018675196135 1104 64-65 0.18575797848541953 1104 66-67 0.21618553752637126 1104 68-69 0.07802648141063884 1104 70-71 0.1157398992995482 1104 72-73 0.14280100803031814 1104 74-75 0.11576980041460416 1104 76-77 0.20380452328292264 1104 78-79 0.35088935759118556 1104 80-81 0.3259761225529729 1104 82-83 0.30997390122999846 1104 84-85 0.1895861660331235 1104 86-87 0.2627146211003826 1104 88-89 0.4584004248464346 1104 90-91 0.45656946905210205 1104 92-93 0.40253950922587123 1104 94-95 0.2831291152216018 1104 96-97 0.18496086159203529 1104 98-99 0.13169747719576108 1104 100-101 -0.15116428009032745 1105 1 0.04379079976745004 1105 2 0.08418673602550797 1105 3 0.07018842790041901 1105 4 0.08719984778758771 1105 5 0.15861255141010844 1105 6 0.07124250332439885 1105 7 0.10790207526267892 1105 8 0.08886770708035385 1105 9 0.10972109821145182 1105 10-11 0.12989533925023977 1105 12-13 0.06540631577436784 1105 14-15 0.12294953097812567 1105 16-17 0.12443485291797884 1105 18-19 0.0865436983986001 1105 20-21 0.12314389637533196 1105 22-23 0.07938488092018758 1105 24-25 0.09307206722148464 1105 26-27 0.13006227508264345 1105 28-29 0.15288171384692362 1105 30-31 0.16912005949139797 1105 32-33 0.1378048049193552 1105 34-35 0.25690530566900804 1105 36-37 0.24020202207272945 1105 38-39 0.3492994540617147 1105 40-41 0.3006705668909646 1105 42-43 0.36156722127659435 1105 44-45 0.38956077665746847 1105 46-47 0.29400593435128997 1105 48-49 0.2344798781611317 1105 50-51 0.2955798211522378 1105 52-53 0.2801561654609017 1105 54-55 0.27048349609143685 1105 56-57 0.24649312813958346 1105 58-59 0.3915572723752021 1105 60-61 0.3515406802933967 1105 62-63 0.32484558693337817 1105 64-65 0.12766434679067373 1105 66-67 0.17580278967636076 1105 68-69 0.06318716163899296 1105 70-71 0.19646670947940947 1105 72-73 -7.157445511083438E-4 1105 74-75 -0.05700204098431527 1105 76-77 -0.05495591623552798 1105 78-79 0.14029095786628432 1105 80-81 0.08344433833858034 1105 82-83 0.08100752101660547 1105 84-85 0.13514465440040624 1105 86-87 0.11414327895163368 1105 88-89 0.3320568592195521 1105 90-91 0.4268106724742573 1105 92-93 0.32136367637278695 1105 94-95 0.08763852551417983 1105 96-97 0.09969232358630009 1105 98-99 0.17196783007775096 1105 100-101 -0.17417683139242257 1106 1 0.055525819376484264 1106 2 0.08614459940068997 1106 3 0.09463283856529614 1106 4 0.06333914794263507 1106 5 0.09043087141740358 1106 6 0.07896755120692944 1106 7 0.08996360811160997 1106 8 0.11158530656960153 1106 9 0.14927054642231496 1106 10-11 0.16635446429204137 1106 12-13 0.17641169685849434 1106 14-15 0.050561028865224955 1106 16-17 0.0012551490296175416 1106 18-19 0.012987014605691627 1106 20-21 0.1516119594890597 1106 22-23 0.06340883738508296 1106 24-25 0.11591051309142841 1106 26-27 0.13749318743481354 1106 28-29 0.18420296760911725 1106 30-31 0.2022555290441872 1106 32-33 0.11507352470775345 1106 34-35 0.03398705558540627 1106 36-37 0.13257425932685862 1106 38-39 0.2587931153270304 1106 40-41 0.204411484411402 1106 42-43 0.20251742344726864 1106 44-45 0.18057786235959128 1106 46-47 0.08558848991022927 1106 48-49 0.032521254745816464 1106 50-51 -0.011407227763939431 1106 52-53 0.10231589549464815 1106 54-55 0.06757330243321036 1106 56-57 0.027701895962529477 1106 58-59 0.06762284863762602 1106 60-61 -0.04666182479887482 1106 62-63 -6.22597786779977E-4 1106 64-65 0.07370377767262681 1106 66-67 0.024939384088547456 1106 68-69 -0.09328077986831573 1106 70-71 -0.008291141735135454 1106 72-73 -0.09956808395462957 1106 74-75 -0.04741717490338715 1106 76-77 3.4156347140168464E-4 1106 78-79 0.1955869174929532 1106 80-81 0.11857339315267268 1106 82-83 0.13195149085821356 1106 84-85 0.1831928713462574 1106 86-87 0.14984692410410005 1106 88-89 0.401843743967067 1106 90-91 0.4453655321708503 1106 92-93 0.4122333142896686 1106 94-95 0.2996311987273259 1106 96-97 0.4819780682736159 1106 98-99 0.43337755251109655 1106 100-101 0.15355323275496602 1107 1 -0.008282533111824364 1107 2 -0.004866042681783256 1107 3 0.03305425573812926 1107 4 0.035021537902572675 1107 5 0.03563191515172548 1107 6 0.10102014159348016 1107 7 0.09227353099318236 1107 8 0.06575218499371971 1107 9 0.07893159413526263 1107 10-11 0.07374315368524975 1107 12-13 0.050114615916811545 1107 14-15 0.1140412178061112 1107 16-17 0.08951408168494623 1107 18-19 0.0367437321570705 1107 20-21 0.08913593648276219 1107 22-23 0.049494122154086995 1107 24-25 0.02915358208854002 1107 26-27 0.019413771856335416 1107 28-29 0.07174988701011387 1107 30-31 0.048692703875495624 1107 32-33 -0.03449311064300531 1107 34-35 0.04463276818673734 1107 36-37 -0.006473064470370105 1107 38-39 0.08619709481826732 1107 40-41 0.058526815021657796 1107 42-43 0.12754512132190854 1107 44-45 0.05543799353789325 1107 46-47 -0.08075405475123887 1107 48-49 -0.01151611524116447 1107 50-51 -0.07777089976883644 1107 52-53 0.013697725398479577 1107 54-55 4.385120687544486E-5 1107 56-57 0.020997485647733072 1107 58-59 -0.05508679486931101 1107 60-61 0.09980322941345321 1107 62-63 -0.0055609509575518246 1107 64-65 -0.028542400165882498 1107 66-67 -0.05507806956561012 1107 68-69 -0.06453742844726662 1107 70-71 0.11926537572360019 1107 72-73 0.10976729773878446 1107 74-75 0.06705701561443078 1107 76-77 -0.01977174007552307 1107 78-79 0.028713430348688007 1107 80-81 -0.08479841469450378 1107 82-83 -0.09114410782971305 1107 84-85 -0.051222845073969125 1107 86-87 -0.07699082806272273 1107 88-89 0.10833753427056081 1107 90-91 0.09565078760841672 1107 92-93 0.05502242855302342 1107 94-95 -0.08199846725736037 1107 96-97 0.07753203302653233 1107 98-99 0.26157362587452226 1107 100-101 -0.12326157700456264 1108 1 -0.09682893907673673 1108 2 -0.09862812335880733 1108 3 -0.0831208571898685 1108 4 -0.01178219991934526 1108 5 0.014251931902677484 1108 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-0.45588951234476127 2316 88-89 -0.674572432666313 2316 90-91 -0.8373328544371788 2316 92-93 -0.6846154334278864 2316 94-95 -0.3608282305418342 2316 96-97 -0.5658093741272019 2316 98-99 -0.6265509747501845 2316 100-101 -0.23974329077613632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 22.0 5 47.0 6 105.0 7 230.0 8 497.0 9 1043.0 10 1773.0 11 2919.0 12 4343.0 13 6375.0 14 7903.0 15 10524.0 16 13862.0 17 19942.0 18 19678.0 19 13602.0 20 12204.0 21 12838.0 22 13183.0 23 14330.0 24 16553.0 25 19469.0 26 23354.0 27 28159.0 28 34516.0 29 42572.0 30 52529.0 31 64781.0 32 81578.0 33 107189.0 34 150604.0 35 223712.0 36 372214.0 37 657041.0 38 634758.0 39 67088.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29657145460076 18.861297683788578 14.085498368233976 23.75663249337669 2 15.93108227681969 20.319987172104305 39.24688097989893 24.502049571177064 3 19.74412301893396 23.79347673680885 29.39890838415008 27.063491860107113 4 13.391642171999967 16.323936582456756 36.00077172386197 34.283649521681305 5 14.196351005492492 37.00858890584713 33.738390736092036 15.056669352568344 6 33.126173556596314 35.22820584818786 16.892259221605926 14.7533613736099 7 29.261769978697057 32.30553968136005 20.169559593885808 18.263130746057087 8 26.657568037452766 35.03597045923061 19.331572914542566 18.974888588774053 9 26.43227445839299 16.938716412348022 20.174428629637344 36.45458049962164 10-11 25.52613958317393 26.280144548126028 26.75901238382499 21.434703484875055 12-13 26.21056858988698 23.45099681608993 27.567986199761528 22.770448394261567 14-15 22.77143901042498 25.063938853003243 25.807628437834524 26.35699369873726 16-17 22.657579547832125 28.297605826573125 26.442864148464267 22.601950477130483 18-19 22.43450135362954 26.83554545138374 28.67937376100339 22.05057943398333 20-21 23.023217246490724 26.074473549237847 28.753010656598622 22.149298547672803 22-23 22.62796503226926 26.01807693589018 28.724125925711697 22.629832106128873 24-25 22.673161431115528 26.189684292724213 28.744769192836184 22.392385083324072 26-27 22.453384342923563 26.45622159883947 28.704554710988877 22.38583934724809 28-29 22.1859444483291 26.75840833051747 28.805069068919554 22.250578152233878 30-31 22.406119776054854 26.27341113630102 28.703290990482238 22.617178097161876 32-33 22.181899121633272 26.494693300170198 28.695442545927268 22.62796503226926 34-35 22.82842502805487 26.57683006529965 28.338053385714623 22.256691520930854 36-37 22.897616965068632 26.254618481690223 28.43514424017954 22.412620313061606 38-39 22.422545691162654 26.51569537186509 28.57961786821384 22.48214106875842 40-41 22.66698277195658 26.355052756935475 28.56581870507285 22.412145766035096 42-43 22.590846681922198 26.508247139588097 28.15666819221968 22.744237986270022 44-45 22.580417231459762 26.637738277091994 28.190993270076508 22.59085122137174 46-47 22.726018844124752 26.85341776512627 27.74135008252748 22.679213308221502 48-49 22.85971546647869 26.86224372589143 27.67742982990994 22.600610977719942 50-51 22.68612135002743 26.99055424504342 27.650894942403465 22.672429462525688 52-53 23.041703504793077 26.95935816543385 27.363433514732506 22.635504815040573 54-55 22.479422832556057 26.745485250665645 27.676825622989465 23.09826629378883 56-57 22.67645403868211 27.17147096498797 27.437199506360315 22.714875489969604 58-59 22.860823293874766 26.76059302991601 27.53829907076164 22.840284605447586 60-61 22.714194311950287 26.781083037910264 27.912249050572953 22.5924735995665 62-63 22.561933410863173 26.852940858816552 27.79305574039012 22.79206998993015 64-65 22.29960781442346 26.766715937744546 27.754547659471257 23.179128588360737 66-67 22.40619982598868 27.01598044834732 27.43138533768831 23.14643438797568 68-69 22.37601125410611 26.81182779460341 27.729070392163862 23.083090559126617 70-71 22.394062262554293 26.8512276291938 27.543747669588512 23.210962438663387 72-73 22.493956721227935 26.7918875274707 27.562183627080184 23.15197212422118 74-75 22.307578818889077 27.12779608038961 27.264458565054895 23.30016653566642 76-77 22.3007781994811 26.881535405315514 27.43620501495733 23.38148138024605 78-79 22.38629971541637 26.88199618199953 27.47234201142252 23.259362091161577 80-81 22.360494147163774 27.17567771151879 27.315786265444387 23.148041875873044 82-83 22.43499850907924 27.01185982159838 27.375410186256182 23.177731483066196 84-85 22.354959425907523 26.913550055536316 27.215686647781144 23.515803870775013 86-87 22.260360639858032 26.777328957565327 27.432081558021654 23.53022884455499 88-89 22.236458591036932 26.773922828057216 27.295658889823006 23.693959691082846 90-91 22.14602053714745 26.80150303367123 27.407979986995663 23.644496442185655 92-93 22.25614276452135 26.632710328615982 27.393012091682845 23.718134815179823 94-95 22.09022945605549 26.458523320301648 27.377215182458876 24.074032041183987 96-97 22.037658513655447 26.328798296350016 27.543220014153153 24.090323175841384 98-99 21.915290912342535 26.40452513022599 27.211901585995836 24.468282371435638 100-101 21.959269608932452 25.773222484306373 27.851641739791315 24.415866166969856 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 468.0 1 319.5 2 259.5 3 347.5 4 295.5 5 294.5 6 568.0 7 1155.0 8 1834.5 9 2259.0 10 2289.0 11 2196.0 12 2315.5 13 2632.0 14 2878.5 15 2969.0 16 3159.0 17 3313.5 18 3468.5 19 3866.0 20 4349.0 21 5012.5 22 5962.0 23 7348.0 24 9059.0 25 11114.5 26 14022.5 27 17618.0 28 21064.5 29 24364.0 30 28536.0 31 34028.5 32 40279.5 33 44807.5 34 48149.5 35 51824.0 36 57190.5 37 62919.5 38 67229.5 39 70477.5 40 72917.5 41 78236.0 42 84279.0 43 89773.0 44 94576.5 45 98699.0 46 100612.5 47 103084.5 48 112578.0 49 114838.5 50 109689.5 51 106324.0 52 102458.0 53 101317.0 54 99706.5 55 92209.5 56 81598.5 57 72600.5 58 65226.0 59 56711.5 60 47785.0 61 40021.0 62 33693.5 63 26699.0 64 20383.5 65 15898.5 66 11519.5 67 9484.5 68 7312.5 69 4570.5 70 3334.0 71 2613.5 72 2166.0 73 1839.5 74 1487.5 75 1249.0 76 879.0 77 481.5 78 286.0 79 170.0 80 94.0 81 59.0 82 32.0 83 17.5 84 7.5 85 6.5 86 4.0 87 2.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3420406092225395 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011348880323128249 16-17 0.002251471418943185 18-19 7.321858272985966E-5 20-21 0.0 22-23 0.0 24-25 4.942254334265527E-4 26-27 0.0012630205520900793 28-29 0.0 30-31 0.002727392206687273 32-33 0.0 34-35 0.010689913078559512 36-37 0.029525393485815912 38-39 0.02308215820558826 40-41 0.01425931898664017 42-43 0.010872959535384162 44-45 0.0032765315771612204 46-47 0.0011898019693602198 48-49 8.603183470758511E-4 50-51 9.152322841232459E-5 52-53 7.321858272985966E-5 54-55 0.0 56-57 0.0 58-59 0.004264982444014326 60-61 0.010781436306971837 62-63 0.004594466066298694 64-65 0.0021782528362133255 66-67 0.004228373152649396 68-69 0.003642624490810519 70-71 5.491393704739476E-5 72-73 0.0 74-75 0.0 76-77 0.21820968118066428 78-79 4.57616142061623E-4 80-81 0.0034229687426209393 82-83 4.942254334265527E-4 84-85 3.660929136492983E-5 86-87 0.11112750393824451 88-89 0.41024371903540374 90-91 0.39962702453957405 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0016657227571043075 100-101 8.786229927583161E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.97924518733102 #Duplication Level Percentage of deduplicated Percentage of total 1 74.08308891183478 38.50783042783104 2 14.243975653578374 14.807822058794478 3 5.000095589972154 7.797035838937654 4 2.2709533612698345 4.72169766297753 5 1.202538836587075 3.1253530517123678 6 0.7516936435256485 2.344348092154673 7 0.48793212242310485 1.7753640398343766 8 0.3336090082156072 1.3872595547793098 9 0.23533480855930278 1.1009273143695846 >10 1.2231249709858132 11.494472989806768 >50 0.09964471194672038 3.596623208823514 >100 0.06159519542625712 6.00612843888387 >500 0.005074168886110626 1.7587460779821231 >1k 0.0011980676536506882 1.036030421716247 >5k 1.4094913572361038E-4 0.5403608213965193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7983 0.2922519729662349 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6768 0.24777168395784516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4176 0.15288040073994702 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3232 0.11832122969145323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.660929136492984E-5 0.0 5 0.0 0.0 0.0 3.660929136492984E-5 0.0 6 0.0 0.0 0.0 3.660929136492984E-5 0.0 7 3.660929136492984E-5 0.0 0.0 3.660929136492984E-5 0.0 8 3.660929136492984E-5 0.0 0.0 3.660929136492984E-5 0.0 9 3.660929136492984E-5 0.0 0.0 3.660929136492984E-5 0.0 10-11 3.660929136492984E-5 0.0 0.0 5.491393704739476E-5 0.0 12-13 3.660929136492984E-5 0.0 0.0 7.321858272985968E-5 0.0 14-15 9.15232284123246E-5 0.0 0.0 7.321858272985968E-5 0.0 16-17 1.0982787409478951E-4 0.0 0.0 9.15232284123246E-5 0.0 18-19 1.2813251977725443E-4 0.0 0.0 1.4643716545971935E-4 0.0 20-21 1.4643716545971935E-4 0.0 0.0 2.19655748189579E-4 0.0 22-23 1.4643716545971935E-4 0.0 0.0 3.2948362228436856E-4 0.0 24-25 2.928743309194387E-4 0.0 0.0 4.027022050142282E-4 0.0 26-27 2.928743309194387E-4 0.0 0.0 8.78622992758316E-4 0.0 28-29 2.928743309194387E-4 0.0 0.0 0.0027640014980522026 0.0 30-31 2.928743309194387E-4 0.0 0.0 0.00810895803733196 0.0 32-33 2.928743309194387E-4 0.0 0.0 0.017920248123133156 0.0 34-35 3.6609291364929835E-4 0.0 0.0 0.0360052380574085 0.0 36-37 4.027022050142282E-4 0.0 0.0 0.06950273965631928 0.0 38-39 5.125300791090177E-4 0.0 0.0 0.11766226244688449 0.0 40-41 5.125300791090177E-4 0.0 0.0 0.16995863516168674 0.0 42-43 5.308347247914827E-4 0.0 0.0 0.2233715912631194 0.0 44-45 5.491393704739476E-4 0.0 0.0 0.2924899333601069 0.0 46-47 5.674440161564124E-4 0.0 0.0 0.3774417939724266 0.0 48-49 6.223579532038073E-4 0.0 0.0 0.46869045269951426 0.0 50-51 6.223579532038073E-4 0.0 0.0 0.5711232499385879 0.0 52-53 6.223579532038073E-4 0.0 0.0 0.6777844203303109 0.0 54-55 6.589672445687371E-4 0.0 0.0 0.7971490148256648 0.0 56-57 6.955765359336669E-4 0.0 0.0 0.9381313958720094 0.0 58-59 6.955765359336669E-4 0.0 0.0 1.0984617874047196 0.0 60-61 6.955765359336669E-4 0.0 0.0 1.2704339335914776 0.0 62-63 6.955765359336669E-4 0.0 0.0 1.449160494035065 0.0 64-65 6.955765359336669E-4 0.0 0.0 1.6394555905499704 3.660929136492984E-5 66-67 7.687951186635266E-4 0.0 0.0 1.8605757103941465 3.660929136492984E-5 68-69 7.687951186635266E-4 0.0 0.0 2.1168590545943378 3.660929136492984E-5 70-71 8.054044100284564E-4 0.0 0.0 2.39876890274998 3.660929136492984E-5 72-73 8.237090557109213E-4 0.0 0.0 2.684852210121224 3.660929136492984E-5 74-75 8.420137013933863E-4 0.0 0.0 2.9840782530924783 3.660929136492984E-5 76-77 8.420137013933863E-4 0.0 0.0 3.31595978396125 3.660929136492984E-5 78-79 8.603183470758512E-4 0.0 0.0 3.682711664855117 3.660929136492984E-5 80-81 9.884508668531057E-4 0.0 0.0 4.101796527755152 3.660929136492984E-5 82-83 9.884508668531057E-4 0.0 0.0 4.532761105703106 3.660929136492984E-5 84-85 0.0010067555125355706 0.0 0.0 4.966965605936855 3.660929136492984E-5 86-87 0.0010250601582180354 0.0 0.0 5.421286911775635 3.660929136492984E-5 88-89 0.0010250601582180354 0.0 0.0 5.91939293008687 3.660929136492984E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9380 0.0 28.014734 1 GTATCAA 13080 0.0 27.369091 1 GTACATG 10360 0.0 26.375603 1 TACATGG 10495 0.0 25.09155 2 ACATGGG 10250 0.0 24.395163 3 ATCAACG 15235 0.0 23.264574 3 TCAACGC 15385 0.0 23.06859 4 CAACGCA 15490 0.0 22.912216 5 TATCAAC 15825 0.0 22.487154 2 AACGCAG 15825 0.0 22.397205 6 GTGTAGC 1475 0.0 21.301107 1 GAGTACT 8905 0.0 21.18088 12-13 CATGGGG 5445 0.0 20.565111 4 ACGCAGA 17665 0.0 20.010576 7 CGCAGAG 17925 0.0 19.693853 8 GTACTTT 9475 0.0 19.681314 14-15 AGAGTAC 14400 0.0 18.996675 10-11 AGTACTT 9580 0.0 18.474985 12-13 CATGGGA 4730 0.0 18.457531 4 GCAGAGT 18895 0.0 18.382517 9 >>END_MODULE