FastQCFastQC Report
Fri 27 May 2016
SRR939172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691613
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76610.2846248699200071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.23145972322172617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37140.13798417528820078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117750.031.3731331
GGTATCA78550.029.8016241
ATCAACG137350.026.4867273
GTACATG100850.026.3193451
TCAACGC139200.026.202774
CAACGCA139100.026.1870675
TATCAAC142050.025.9104842
AACGCAG144400.025.2446216
TACATGG103400.025.2421762
ACATGGG103250.024.223653
ACGCAGA162200.022.386697
CGCAGAG162700.022.288388
GAGTACT81250.021.88024112-13
CATGGGG57000.020.5266384
GCAGAGT172400.020.4302169
GTACTTT88500.020.00773414-15
AGAGTAC133900.019.37534310-11
AGTACTT87400.018.93220912-13
GAGTACA82700.018.717651
AGTACAT80000.017.763442
Produced by FastQC (version 0.11.5)