##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2691613 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20408840349634 33.0 31.0 34.0 31.0 34.0 2 32.30464966546082 34.0 31.0 34.0 31.0 34.0 3 32.371357249351966 34.0 31.0 34.0 30.0 34.0 4 35.79812439604059 37.0 35.0 37.0 35.0 37.0 5 35.8317087188983 37.0 35.0 37.0 35.0 37.0 6 35.91641777625535 37.0 37.0 37.0 35.0 37.0 7 35.84795956922485 37.0 37.0 37.0 35.0 37.0 8 35.8469237590991 37.0 37.0 37.0 35.0 37.0 9 37.54245205384281 39.0 38.0 39.0 35.0 39.0 10-11 37.456075780582125 39.0 38.0 39.0 35.0 39.0 12-13 37.366102779262846 39.0 37.5 39.0 35.0 39.0 14-15 38.694090309416694 40.0 38.0 41.0 35.0 41.0 16-17 38.61109750918873 40.0 38.0 41.0 34.0 41.0 18-19 38.50366787498797 40.0 38.0 41.0 34.0 41.0 20-21 38.486692180488056 40.0 38.0 41.0 34.0 41.0 22-23 38.529466531778525 40.0 38.0 41.0 34.0 41.0 24-25 38.47721050537354 40.0 38.0 41.0 34.0 41.0 26-27 38.37456109032019 40.0 38.0 41.0 34.0 41.0 28-29 38.24088455509763 40.0 38.0 41.0 34.0 41.0 30-31 38.04522306884385 40.0 38.0 41.0 33.5 41.0 32-33 37.859877330061934 40.0 38.0 41.0 33.0 41.0 34-35 37.56250991505837 40.0 37.5 41.0 32.5 41.0 36-37 37.310707371379166 40.0 37.0 41.0 31.5 41.0 38-39 37.13504708886456 40.0 37.0 41.0 31.0 41.0 40-41 36.95346322075276 40.0 37.0 41.0 31.0 41.0 42-43 36.751287982336244 40.0 36.5 41.0 30.0 41.0 44-45 36.49568084267686 40.0 36.0 41.0 30.0 41.0 46-47 36.37513788200607 40.0 36.0 41.0 30.0 41.0 48-49 36.26549396217064 40.0 36.0 41.0 29.5 41.0 50-51 35.13673288098995 38.5 34.5 40.0 27.5 40.5 52-53 35.271707708351826 38.5 35.0 40.0 27.5 40.5 54-55 35.79861313643529 39.0 35.0 41.0 28.0 41.0 56-57 35.708873266699186 39.0 35.0 41.0 28.0 41.0 58-59 35.51195844276276 39.0 35.0 41.0 28.0 41.0 60-61 35.2202194371925 39.0 35.0 41.0 27.0 41.0 62-63 34.850379122110056 38.0 35.0 40.0 26.0 41.0 64-65 34.48414203676383 37.5 34.0 40.0 26.0 41.0 66-67 34.10972156844242 37.0 34.0 40.0 25.5 41.0 68-69 33.70553549117202 36.5 34.0 39.0 24.5 41.0 70-71 33.28193113200152 36.0 33.5 39.0 23.5 41.0 72-73 32.845790052284634 35.0 33.0 38.5 22.5 40.0 74-75 32.41167415226484 35.0 33.0 37.5 21.5 39.5 76-77 31.983968163328086 35.0 32.5 37.0 20.0 39.0 78-79 31.562122972358953 35.0 32.0 36.5 19.0 39.0 80-81 31.126976277793275 35.0 31.5 36.0 17.5 37.5 82-83 30.751043705020003 35.0 31.0 35.5 12.5 37.0 84-85 30.393415212365227 35.0 31.0 35.0 7.5 37.0 86-87 30.002638195015408 34.0 31.0 35.0 4.0 36.0 88-89 29.604624067427228 34.0 30.5 35.0 2.0 36.0 90-91 29.218535688451496 34.0 30.0 35.0 2.0 36.0 92-93 28.827911181882385 34.0 29.5 35.0 2.0 35.0 94-95 28.37071674122543 34.0 29.0 35.0 2.0 35.0 96-97 27.806419422108604 34.0 29.0 35.0 2.0 35.0 98-99 27.133856724573704 34.0 27.0 35.0 2.0 35.0 100-101 24.252187257232002 31.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1595631716411532 1101 2 0.33790651878542377 1101 3 0.23330102765941518 1101 4 0.27700706121824936 1101 5 0.1731167393856481 1101 6 0.19580658617834246 1101 7 0.19157192336763273 1101 8 0.29483454030151535 1101 9 0.3238625070404737 1101 10-11 0.28376343486046096 1101 12-13 0.3346310272273172 1101 14-15 0.47982473122173985 1101 16-17 0.43346222548457547 1101 18-19 0.4845615458579928 1101 20-21 0.42943723709262116 1101 22-23 0.5050664953276893 1101 24-25 0.45531778602372697 1101 26-27 0.3577143011728694 1101 28-29 0.4081521735490483 1101 30-31 0.49719487342113666 1101 32-33 0.3943918823995176 1101 34-35 0.47178173440185844 1101 36-37 0.3643196398063253 1101 38-39 0.4397641310764513 1101 40-41 0.3982139862253291 1101 42-43 0.4933308144475461 1101 44-45 0.497108650490361 1101 46-47 0.5368883785590555 1101 48-49 0.3770456966452329 1101 50-51 0.41701172277622334 1101 52-53 0.3972255574417787 1101 54-55 0.4792501317629032 1101 56-57 0.4417340971178305 1101 58-59 0.3666212630934993 1101 60-61 0.3413606854815825 1101 62-63 0.28473351748879594 1101 64-65 0.3066369501033037 1101 66-67 0.33758229961359376 1101 68-69 0.18492794933263923 1101 70-71 0.14657550951900333 1101 72-73 0.13420063223501444 1101 74-75 0.02652256350418014 1101 76-77 -0.1359435045712587 1101 78-79 -0.20481859819336634 1101 80-81 -0.195825601021685 1101 82-83 -0.29258777192864827 1101 84-85 -0.45364668505388295 1101 86-87 -0.5129939823859857 1101 88-89 -0.5477989020882355 1101 90-91 -0.5261949596484747 1101 92-93 -0.5256957580413726 1101 94-95 -0.7302716644235154 1101 96-97 -0.6376015445406473 1101 98-99 -0.6521756449602307 1101 100-101 -0.8979272152515385 1102 1 0.113888069114779 1102 2 0.18094769340937233 1102 3 0.18956252381253336 1102 4 0.14466820716977935 1102 5 0.2441747332154236 1102 6 0.23243193233575 1102 7 0.15986396244849743 1102 8 0.24740804555665363 1102 9 0.2665970151155861 1102 10-11 0.2541880379634023 1102 12-13 0.32781863791411325 1102 14-15 0.3349937590146155 1102 16-17 0.3298889703734389 1102 18-19 0.3618945420797033 1102 20-21 0.30123757453726085 1102 22-23 0.42747401793767636 1102 24-25 0.4022669898857103 1102 26-27 0.2958574565453418 1102 28-29 0.23894128755899402 1102 30-31 0.4392854816476017 1102 32-33 0.345798625374826 1102 34-35 0.24227279281068093 1102 36-37 0.2700518566239154 1102 38-39 0.18102743894837658 1102 40-41 0.13536154159610447 1102 42-43 0.14572326309598083 1102 44-45 0.08502888567951317 1102 46-47 0.2696835820959649 1102 48-49 0.20967448333926342 1102 50-51 0.10275593574674247 1102 52-53 0.08138850300836964 1102 54-55 0.12398609920345649 1102 56-57 0.0642109953015435 1102 58-59 -0.032850245274794077 1102 60-61 -0.055471726878685956 1102 62-63 -0.2068010678965706 1102 64-65 -0.1588243503918747 1102 66-67 -0.1992768600946988 1102 68-69 -0.18374088115003673 1102 70-71 -0.27228042944125974 1102 72-73 -0.23420441352673294 1102 74-75 -0.31645674755084485 1102 76-77 -0.3269217212943225 1102 78-79 -0.3863977791427722 1102 80-81 -0.2386251042687526 1102 82-83 -0.4673187326468202 1102 84-85 -0.5641908308582444 1102 86-87 -0.5701112515051925 1102 88-89 -0.5092275432299651 1102 90-91 -0.3473696681308134 1102 92-93 -0.4085836992948053 1102 94-95 -0.5603659660203242 1102 96-97 -0.6224507338397096 1102 98-99 -0.6470056181283503 1102 100-101 -0.7647307246450019 1103 1 0.091490224034672 1103 2 0.1535815896217514 1103 3 0.2656031809860053 1103 4 0.2148593406618744 1103 5 0.17307935328643254 1103 6 0.19139371138269468 1103 7 0.17330533831162143 1103 8 0.2368426885924464 1103 9 0.11197105974002852 1103 10-11 0.23789520000662634 1103 12-13 0.25751806447100734 1103 14-15 0.3182505585159987 1103 16-17 0.3889979419844707 1103 18-19 0.39641714759195423 1103 20-21 0.4109545077796284 1103 22-23 0.2594426415685831 1103 24-25 0.3906342922307644 1103 26-27 0.417677095428715 1103 28-29 0.40189385326327454 1103 30-31 0.4897863200076742 1103 32-33 0.523137772276975 1103 34-35 0.44226183954886267 1103 36-37 0.34658735659027684 1103 38-39 0.34566219214692495 1103 40-41 0.3741532133362995 1103 42-43 0.2225192505325353 1103 44-45 0.3259868383128648 1103 46-47 0.39582132668406445 1103 48-49 0.2514192916136295 1103 50-51 0.2634916242723051 1103 52-53 0.28979960728064214 1103 54-55 0.26005674718619076 1103 56-57 0.2500855317107593 1103 58-59 0.28352286100168556 1103 60-61 0.28165104584224565 1103 62-63 0.06398673109644193 1103 64-65 0.14328449537710952 1103 66-67 0.059904280723657166 1103 68-69 0.15923299059449647 1103 70-71 0.2047086393517148 1103 72-73 0.23838648510963623 1103 74-75 0.126841867093205 1103 76-77 0.06268339230360098 1103 78-79 0.15404718646194837 1103 80-81 0.18626192979366607 1103 82-83 0.024449918895452782 1103 84-85 0.0281458149564493 1103 86-87 -0.055791390683896935 1103 88-89 -0.25196720791930005 1103 90-91 -0.10269668786317965 1103 92-93 0.12553580394233776 1103 94-95 0.10895561563068057 1103 96-97 0.07443813206771566 1103 98-99 -0.04049228230041635 1103 100-101 -0.025956381669278272 1104 1 0.229241704867718 1104 2 0.3493468722980495 1104 3 0.34911360108868195 1104 4 0.32153077193176927 1104 5 0.31104175239102716 1104 6 0.27042022400325294 1104 7 0.24791701610039496 1104 8 0.28204615688527923 1104 9 0.3730129924773635 1104 10-11 0.3785922241031088 1104 12-13 0.37867598639918043 1104 14-15 0.47469746843115956 1104 16-17 0.4561648391282489 1104 18-19 0.46854044791138705 1104 20-21 0.4887836142896589 1104 22-23 0.4474133752047109 1104 24-25 0.3880691771613698 1104 26-27 0.39450163539076755 1104 28-29 0.4278246424676482 1104 30-31 0.4751485567210523 1104 32-33 0.3703010536789506 1104 34-35 0.31840497429127623 1104 36-37 0.477857549768153 1104 38-39 0.22509676202165707 1104 40-41 0.33303948028377306 1104 42-43 0.3255290384090159 1104 44-45 0.3596302408880021 1104 46-47 0.5763642835291662 1104 48-49 0.6486189242598996 1104 50-51 0.5456256484731057 1104 52-53 0.5653365766546727 1104 54-55 0.5599257029768765 1104 56-57 0.5601371529445345 1104 58-59 0.5483678422392941 1104 60-61 0.5511234541377306 1104 62-63 0.4418281367671639 1104 64-65 0.5079268576335565 1104 66-67 0.5689721805485775 1104 68-69 0.44627240640902954 1104 70-71 0.5323929928458071 1104 72-73 0.5045747708110326 1104 74-75 0.4713457633545488 1104 76-77 0.43831887830723204 1104 78-79 0.42242976225156426 1104 80-81 0.39489261031433287 1104 82-83 0.22960229809584476 1104 84-85 0.2332927317788851 1104 86-87 0.4201766131728917 1104 88-89 0.3467784010336423 1104 90-91 0.48904289273896495 1104 92-93 0.30507570960883257 1104 94-95 0.2588133118745368 1104 96-97 0.2805878357601408 1104 98-99 0.08384941305254756 1104 100-101 -0.18043428053563915 1105 1 0.09675881169272316 1105 2 0.13779291731827215 1105 3 0.14627922576977426 1105 4 0.18769605017951108 1105 5 0.1950020226627487 1105 6 0.18081147884392124 1105 7 0.1997523743232179 1105 8 0.3001620697237257 1105 9 0.29723237609455566 1105 10-11 0.29706776295467563 1105 12-13 0.25064217692141 1105 14-15 0.2132594612017158 1105 16-17 0.24650602897909835 1105 18-19 0.3728654265614182 1105 20-21 0.317450671700918 1105 22-23 0.2928253824771261 1105 24-25 0.388963290748535 1105 26-27 0.42618048093520855 1105 28-29 0.2803628709847459 1105 30-31 0.3293041455771828 1105 32-33 0.2842521461524896 1105 34-35 0.2618962234923288 1105 36-37 0.23053082686649162 1105 38-39 0.40623541324115564 1105 40-41 0.3611301222865251 1105 42-43 0.32096850522392373 1105 44-45 0.2651417201852553 1105 46-47 0.30723105842267984 1105 48-49 0.4167163740807709 1105 50-51 0.48803429346217087 1105 52-53 0.4946336257944992 1105 54-55 0.4799460928621002 1105 56-57 0.5404428199495825 1105 58-59 0.3898571296608111 1105 60-61 0.5117644968215416 1105 62-63 0.4290512250727545 1105 64-65 0.526191649531853 1105 66-67 0.5137315033653778 1105 68-69 0.5061813736616756 1105 70-71 0.3648155958761592 1105 72-73 0.4966358411020906 1105 74-75 0.416056410943213 1105 76-77 0.44227651004588964 1105 78-79 0.26567723248246367 1105 80-81 0.348335090293709 1105 82-83 0.4980238577978575 1105 84-85 0.4203168894624518 1105 86-87 0.2774440597174248 1105 88-89 0.4660825601136871 1105 90-91 0.3822611914021081 1105 92-93 0.3506323662316184 1105 94-95 0.3836803781480427 1105 96-97 0.4564620092883409 1105 98-99 0.32416203557984247 1105 100-101 0.05622954618323561 1106 1 0.15888322659826315 1106 2 0.21347797969340832 1106 3 0.20691266761667038 1106 4 0.2718559730429817 1106 5 0.26128236171521735 1106 6 0.1937797358066078 1106 7 0.24496173454469528 1106 8 0.26991661624830954 1106 9 0.18103983144356306 1106 10-11 0.2401318439996274 1106 12-13 0.22373041150417805 1106 14-15 0.23703618447923702 1106 16-17 0.32100107008686507 1106 18-19 0.24454024383781103 1106 20-21 0.34265879038540703 1106 22-23 0.37766637706112505 1106 24-25 0.407077396059897 1106 26-27 0.35435756603208546 1106 28-29 0.40751504311014486 1106 30-31 0.3430365927433314 1106 32-33 0.26010650902634325 1106 34-35 0.35646296739227523 1106 36-37 0.5174172939520574 1106 38-39 0.39895444374588607 1106 40-41 0.5769192480348764 1106 42-43 0.6581780944041711 1106 44-45 0.49377051513655346 1106 46-47 0.43426431710255287 1106 48-49 0.3906483701014096 1106 50-51 0.35086614259446947 1106 52-53 0.20715372015131095 1106 54-55 0.21795558658911318 1106 56-57 0.2508603633509736 1106 58-59 0.28646442828592455 1106 60-61 0.287772681291365 1106 62-63 0.3300207525953667 1106 64-65 0.2322255193059135 1106 66-67 0.16609243912954952 1106 68-69 0.288278893973704 1106 70-71 0.23451354766260124 1106 72-73 0.2776192028940301 1106 74-75 0.3294848427852486 1106 76-77 0.28263452416716817 1106 78-79 0.23948644533188812 1106 80-81 0.1608626016247463 1106 82-83 0.3808375192194333 1106 84-85 0.34344744916990066 1106 86-87 0.35138242466157976 1106 88-89 0.39404044137438277 1106 90-91 0.3411578519900793 1106 92-93 0.19089621702789472 1106 94-95 0.3110939986150214 1106 96-97 0.41140187536358397 1106 98-99 0.39674390043289876 1106 100-101 0.06699463932269367 1107 1 0.04182597729844417 1107 2 0.13909234362726863 1107 3 0.044241552568507814 1107 4 0.09896987537682378 1107 5 0.12446972150323887 1107 6 0.057604461687809305 1107 7 0.10933586563910325 1107 8 0.13356526468657393 1107 9 0.17670328221894493 1107 10-11 0.13156460997230823 1107 12-13 0.17237270029298202 1107 14-15 0.1676148892280409 1107 16-17 0.07114079555551456 1107 18-19 0.13721722813406956 1107 20-21 0.25017773311119385 1107 22-23 0.2925697524236668 1107 24-25 0.27525221558441615 1107 26-27 0.20673001886403597 1107 28-29 0.17547992414500158 1107 30-31 0.21694949746067493 1107 32-33 0.1790157629779614 1107 34-35 0.10636597689983063 1107 36-37 0.07690796153621449 1107 38-39 -0.08066727776529348 1107 40-41 0.12262029624833559 1107 42-43 0.06672726920656658 1107 44-45 0.05913533483231248 1107 46-47 0.16221244664946965 1107 48-49 0.0439807641474701 1107 50-51 0.10360686350004045 1107 52-53 0.09292639810084324 1107 54-55 0.10325615592174131 1107 56-57 -0.004304030084810506 1107 58-59 0.07984424336569873 1107 60-61 0.025601386429364936 1107 62-63 0.07527304463794593 1107 64-65 0.059012643078546034 1107 66-67 0.13740722854496568 1107 68-69 0.09576732283670708 1107 70-71 0.12583912590807955 1107 72-73 0.06589284997007638 1107 74-75 -0.002283124197504094 1107 76-77 0.01134012922125649 1107 78-79 0.050995620507926276 1107 80-81 -0.10608543606921117 1107 82-83 -0.07431309627320104 1107 84-85 -0.13877604619308315 1107 86-87 -0.2108572928672352 1107 88-89 -0.18235895395295287 1107 90-91 -0.0762951434281689 1107 92-93 -0.049714465003642516 1107 94-95 -0.058741413345476445 1107 96-97 0.020269811054060227 1107 98-99 -0.2504003289441634 1107 100-101 -0.13519862240685043 1108 1 0.10902170167012315 1108 2 0.12514745735779087 1108 3 0.19189331560350809 1108 4 0.21286566742291058 1108 5 0.2528283704609322 1108 6 0.19690710512521292 1108 7 0.0264391469469345 1108 8 0.13476854578762953 1108 9 0.18843641759995933 1108 10-11 0.21565944039785023 1108 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-1.0113306884560416 2316 88-89 -1.1095648012856394 2316 90-91 -1.3130034601933964 2316 92-93 -1.2615070437469207 2316 94-95 -1.2179622157255245 2316 96-97 -1.4243926924487802 2316 98-99 -1.7157283663904863 2316 100-101 -1.4998433708060652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7589.0 3 1980.0 4 1997.0 5 2295.0 6 2568.0 7 3188.0 8 3858.0 9 4634.0 10 5863.0 11 7203.0 12 9628.0 13 15837.0 14 13057.0 15 12806.0 16 15570.0 17 21279.0 18 18728.0 19 13553.0 20 13175.0 21 14296.0 22 14902.0 23 16348.0 24 18525.0 25 21011.0 26 24029.0 27 28250.0 28 33488.0 29 39501.0 30 48507.0 31 59800.0 32 76225.0 33 99802.0 34 141073.0 35 212524.0 36 351930.0 37 592379.0 38 610948.0 39 113210.0 40 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0693006266046 18.40853844354833 13.674930258113054 23.847230671734014 2 15.713193706955714 19.86980934508521 39.83491824956805 24.58207869839103 3 19.651827163432927 23.713843590048803 29.281856326627782 27.35247291989049 4 13.142491920545865 15.850053069374114 36.334533858186276 34.67292115189374 5 14.234756818936024 36.83486634453838 33.751259321209126 15.17911751531647 6 33.09349441902332 34.85774560591735 17.18132513478855 14.867434840270777 7 29.3410601936549 32.12566745497003 20.330938675947824 18.202333675427244 8 26.641868129988556 34.696323277333356 19.653410455691702 19.008398136986383 9 26.56735875077573 16.93060902137381 20.268623673641795 36.233408554208665 10-11 25.530523334177367 26.127834994814958 26.893395605895147 21.44824606511253 12-13 26.17885138925117 23.383839444890754 27.607508074923086 22.829801090934986 14-15 22.8661683388312 24.955382505777965 25.855131458010476 26.32331769738036 16-17 22.721774860725503 28.146703833799407 26.47617209765631 22.65534920781878 18-19 22.532139526374266 26.70795385414017 28.680483353487933 22.079423265997622 20-21 23.14225463615942 26.025744810524603 28.650890716005 22.181109837310974 22-23 22.711660183415272 25.967746134221898 28.62674694493823 22.6938467374246 24-25 22.716431561151083 26.13849023781943 28.712584599768913 22.432493601260575 26-27 22.512018448434578 26.40052816082869 28.558437090280165 22.52901630045656 28-29 22.272160937837054 26.667509688553004 28.75142259943319 22.30890677417675 30-31 22.46134279402905 26.26330410335364 28.563677473577737 22.711675629039576 32-33 22.221561108251795 26.437712155605077 28.64502295222427 22.695703783918866 34-35 22.950104286881476 26.503296002314464 28.23103388946168 22.315565821342375 36-37 22.948273144215563 26.246478415516712 28.298354688834255 22.506893751433473 38-39 22.476954663733693 26.502533516457355 28.444942817315116 22.575569002493836 40-41 22.745918700704717 26.326802632193026 28.458001586783034 22.469277080319223 42-43 22.65950078736658 26.450232512628396 28.05611301030607 22.83415368969895 44-45 22.63196985200343 26.599100482925962 28.079794556171823 22.689135108898785 46-47 22.74711221384053 26.796661085375085 27.70825544362678 22.747971257157605 48-49 22.8856055894531 26.80291051981839 27.62875931248681 22.682724578241697 50-51 22.699963395138276 26.945156205644622 27.62064663608791 22.734233763129193 52-53 23.013842254069296 26.91996166996013 27.383548972490107 22.682647103480463 54-55 22.49793926518697 26.70697378058095 27.65662985169059 23.138457102541494 56-57 22.699910949480167 27.14056353857269 27.40450405207869 22.75502145986846 58-59 22.83097547512615 26.732618375296408 27.543064271742047 22.8933418778354 60-61 22.781408470990076 26.698881774682924 27.938282216211867 22.581427538115125 62-63 22.586618284668457 26.756503478249666 27.901337469478843 22.75554076760303 64-65 22.351804958878965 26.711583480165267 27.819238525910716 23.11737303504505 66-67 22.39397370106588 26.993961634421392 27.489104996804954 23.122959667707775 68-69 22.46667379060552 26.718693838266162 27.755040124464674 23.059592246663644 70-71 22.389743484364626 26.763725963626584 27.704454534161144 23.14207601784765 72-73 22.508626560429633 26.70505549875285 27.685683514593524 23.100634426223994 74-75 22.390014031700588 27.04785870869502 27.410419023339845 23.151708236264547 76-77 22.46013581498327 26.805158310133535 27.485335014524924 23.249370860358265 78-79 22.484439233667263 26.824856900691135 27.5218896203061 23.168814245335504 80-81 22.472283103769602 27.122650008056343 27.42931376958564 22.975753118588415 82-83 22.639522324916456 26.936036115439144 27.438064770566278 22.98637678907813 84-85 22.542538367014423 26.800473599763386 27.34808966428827 23.308898368933917 86-87 22.57502658014132 26.669880806691886 27.59158711919069 23.163505493976103 88-89 22.48249612461874 26.74792720090936 27.395028568856855 23.37454810561504 90-91 22.470254271763384 26.730680079000678 27.58338908731204 23.2156765619239 92-93 22.573808569835503 26.511068833828798 27.615589296438152 23.299533299897547 94-95 22.461072369483986 26.38923988273848 27.63210365634418 23.517584091433353 96-97 22.506389849161458 26.286244322728287 27.706244008932714 23.50112181917754 98-99 22.40442904919827 26.321332076891967 27.448001352324404 23.82623752158536 100-101 22.65852559053427 25.67249404582502 27.991214300626734 23.677766063013976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2401.0 1 1334.5 2 348.0 3 466.0 4 428.0 5 413.0 6 677.5 7 1343.0 8 2127.0 9 2571.0 10 2569.5 11 2571.0 12 2863.0 13 3181.0 14 3543.0 15 3812.0 16 4025.0 17 4216.0 18 4371.0 19 4567.5 20 4825.5 21 5356.0 22 6139.5 23 7369.5 24 9040.5 25 11198.0 26 14008.5 27 17165.5 28 20326.0 29 23760.5 30 27948.5 31 33440.0 32 39362.0 33 43410.5 34 47010.0 35 50806.5 36 55713.0 37 61581.5 38 65921.5 39 68778.5 40 71547.0 41 77069.0 42 83111.0 43 88352.0 44 92644.5 45 96949.0 46 99042.0 47 101078.0 48 110178.0 49 112471.5 50 107395.0 51 104198.0 52 100390.0 53 98465.5 54 96623.0 55 89648.0 56 79613.0 57 70845.5 58 63320.0 59 55098.5 60 46717.5 61 39307.0 62 33230.5 63 26731.5 64 20545.0 65 16165.5 66 12133.5 67 9990.5 68 7630.0 69 4912.0 70 3704.5 71 2909.5 72 2402.0 73 1963.0 74 1530.0 75 1325.5 76 956.5 77 558.0 78 357.5 79 216.0 80 130.0 81 85.5 82 61.0 83 55.0 84 42.5 85 29.5 86 21.5 87 15.0 88 13.5 89 10.0 90 5.5 91 5.5 92 6.5 93 8.0 94 5.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39589643830669563 2 0.12067113660098983 3 0.12234299656005525 4 0.13456615048300036 5 0.1358664860067179 6 0.07541946037561864 7 0.08519055302526775 8 0.08199544288127603 9 0.08173537577653252 10-11 0.07972914382565399 12-13 0.08428031815866545 14-15 0.07746284477003194 16-17 0.07950622916444526 18-19 0.0892030169270248 20-21 0.08812559606451596 22-23 0.09782238382709552 24-25 0.11136444949552554 26-27 0.11286912345868444 28-29 0.10673897027544452 30-31 0.096336286085704 32-33 0.09470157857017336 34-35 0.347319618384961 36-37 0.540605205874693 38-39 0.5205243101441404 40-41 0.491824047513517 42-43 0.5207472248053491 44-45 0.5308155370032765 46-47 0.5281591372905392 48-49 0.5360354553199141 50-51 0.5339920709255008 52-53 0.5327474640670854 54-55 0.526450124887939 56-57 0.4962823407376915 58-59 0.48623260476153146 60-61 0.431246988330046 62-63 0.5111247419298391 64-65 0.5393048703509754 66-67 0.5503205698590399 68-69 0.564382769736957 70-71 0.5599059002910152 72-73 0.5463638346225851 74-75 0.5504877558549465 76-77 0.5255956186866388 78-79 0.4979542006967569 80-81 0.5052175034078079 82-83 0.5192797032857249 84-85 0.5136139556466699 86-87 0.5150071722792243 88-89 0.525131213142454 90-91 0.5451749564294718 92-93 0.551360838278014 94-95 0.5457879717477959 96-97 0.5465495968402589 98-99 0.5476455939245353 100-101 0.5231064049698081 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2691613.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.64329978856301 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25190629147794 40.36760568854603 2 13.817531600860217 14.824359800057755 3 4.784031106917566 7.698936445985701 4 2.080708520066988 4.464642836582828 5 1.1199197662813192 3.003809588088312 6 0.6502216231962104 2.0928020077272222 7 0.430318560188811 1.615859527015383 8 0.3159906218793212 1.356062372787751 9 0.23672660635614884 1.1428916681422836 >10 1.1574099672837137 11.11954842418168 >50 0.09118426981512626 3.3760564428656394 >100 0.058499184286739454 5.910519047802438 >500 0.004163911042378868 1.4735078015963674 >1k 0.0012491733127018392 1.0362689968653631 >5k 1.3879703474464883E-4 0.5171293517551644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7661 0.2846248699200071 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6230 0.23145972322172617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3714 0.13798417528820078 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.7152443534787503E-5 0.0 0.0 0.0 0.0 7 7.430488706957501E-5 0.0 0.0 0.0 0.0 8 7.430488706957501E-5 0.0 0.0 0.0 0.0 9 7.430488706957501E-5 0.0 0.0 0.0 0.0 10-11 7.430488706957501E-5 0.0 0.0 0.0 0.0 12-13 9.288110883696877E-5 0.0 0.0 0.0 0.0 14-15 1.4860977413915001E-4 0.0 0.0 0.0 0.0 16-17 1.4860977413915001E-4 0.0 0.0 0.0 0.0 18-19 2.0433843944133129E-4 0.0 0.0 0.0 0.0 20-21 2.6006710474351253E-4 0.0 0.0 0.0 0.0 22-23 2.6006710474351253E-4 0.0 0.0 0.0 0.0 24-25 3.343719918130875E-4 0.0 0.0 2.4149088297611878E-4 0.0 26-27 3.343719918130875E-4 0.0 0.0 5.387104312544189E-4 0.0 28-29 4.272531006500563E-4 0.0 0.0 0.002191994168552463 0.0 30-31 4.4582932241745007E-4 0.0 0.0 0.007894894251142344 0.0 32-33 4.4582932241745007E-4 0.0 0.0 0.018799136428602477 0.0 34-35 4.4582932241745007E-4 0.0 0.0 0.03770973018780932 0.0 36-37 4.4582932241745007E-4 0.0 0.0 0.07084970982083977 0.0 38-39 4.4582932241745007E-4 0.0 0.0 0.12094977992750072 0.0 40-41 4.4582932241745007E-4 0.0 0.0 0.17567532925424273 0.0 42-43 5.015579877196313E-4 0.0 0.0 0.231924128765911 0.0 44-45 5.201342094870251E-4 0.0 0.0 0.30106482618415054 0.0 46-47 5.201342094870251E-4 0.0 0.0 0.3862925316529531 0.0 48-49 5.201342094870251E-4 0.0 0.0 0.4778547287444369 0.0 50-51 5.201342094870251E-4 0.0 0.0 0.5806741162269613 0.0 52-53 5.201342094870251E-4 0.0 0.0 0.685444006995062 0.0 54-55 5.201342094870251E-4 0.0 0.0 0.802864304786758 0.0 56-57 5.201342094870251E-4 0.0 0.0 0.9452696208555984 0.0 58-59 5.201342094870251E-4 0.0 0.0 1.1030746247696084 0.0 60-61 5.201342094870251E-4 0.0 0.0 1.2762793165287878 0.0 62-63 5.572866530218126E-4 0.0 0.0 1.4549825699311156 0.0 64-65 5.944390965566001E-4 0.0 0.0 1.6478037518766628 0.0 66-67 6.315915400913876E-4 0.0 0.0 1.8742478952211927 0.0 68-69 6.315915400913876E-4 0.0 0.0 2.124934007972171 0.0 70-71 6.501677618587812E-4 0.0 0.0 2.4024441849552667 0.0 72-73 6.873202053935689E-4 0.0 0.0 2.6917316865388896 0.0 74-75 7.244726489283562E-4 0.0 0.0 2.9931494609366203 0.0 76-77 7.4304887069575E-4 0.0 0.0 3.3271127758708254 0.0 78-79 7.4304887069575E-4 0.0 0.0 3.7002161900689288 0.0 80-81 9.102348666022938E-4 0.0 0.0 4.111345130224887 0.0 82-83 9.288110883696876E-4 0.0 0.0 4.537502233790668 0.0 84-85 9.473873101370814E-4 0.0 0.0 4.979987836289987 0.0 86-87 9.659635319044751E-4 0.0 0.0 5.435439641582947 0.0 88-89 9.659635319044751E-4 0.0 0.0 5.938000745278018 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11775 0.0 31.373133 1 GGTATCA 7855 0.0 29.801624 1 ATCAACG 13735 0.0 26.486727 3 GTACATG 10085 0.0 26.319345 1 TCAACGC 13920 0.0 26.20277 4 CAACGCA 13910 0.0 26.187067 5 TATCAAC 14205 0.0 25.910484 2 AACGCAG 14440 0.0 25.244621 6 TACATGG 10340 0.0 25.242176 2 ACATGGG 10325 0.0 24.22365 3 ACGCAGA 16220 0.0 22.38669 7 CGCAGAG 16270 0.0 22.28838 8 GAGTACT 8125 0.0 21.880241 12-13 CATGGGG 5700 0.0 20.526638 4 GCAGAGT 17240 0.0 20.430216 9 GTACTTT 8850 0.0 20.007734 14-15 AGAGTAC 13390 0.0 19.375343 10-11 AGTACTT 8740 0.0 18.932209 12-13 GAGTACA 8270 0.0 18.71765 1 AGTACAT 8000 0.0 17.76344 2 >>END_MODULE