FastQCFastQC Report
Fri 27 May 2016
SRR939172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691613
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74790.2778631251966758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67400.2504074694244678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.1459719506481801No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30830.11454098341774988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88250.028.6740951
GTACATG96850.026.4231471
GTATCAA122100.026.266931
TACATGG97300.025.9930152
ACATGGG97800.024.4045853
ATCAACG143500.022.1546633
CAACGCA144150.022.0876835
TATCAAC144650.022.076942
TCAACGC144350.021.9913374
AACGCAG148650.021.4828766
GAGTACT81600.020.79105812-13
CATGGGG51700.020.7424554
GTATACG1200.004676073319.8563161
ACGCAGA166100.019.1973787
CGCAGAG169450.018.7898468
GTACTTT90300.018.68282514-15
AGAGTAC135250.017.87662310-11
CATGGGA49650.017.6805084
GTGTAAG10800.017.6500571
GCAGAGT179550.017.6017279
Produced by FastQC (version 0.11.5)