FastQCFastQC Report
Fri 27 May 2016
SRR939171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711576
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74320.20023838929877766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59380.15998594667063262No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42230.11377916011958261No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41820.11267450807958668No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39210.10564245484936856No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA38730.10434920368059283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100800.028.211851
GTATCAA139450.026.3504281
TACATGG122150.023.6600672
GTACATG128050.023.5057931
ACATGGG122250.022.7880973
TCAACGC160650.022.4726284
ATCAACG161550.022.2887783
TATCAAC163800.022.156162
CAACGCA162900.022.074985
AACGCAG167250.021.63436
GAGTACT91200.020.17478412-13
CATGGGG77400.019.6491494
TAAGGTG18850.019.604825
GAGTACA91900.019.2689741
ACGCAGA188350.019.0346937
CGCAGAG188950.018.974768
GTACTTT97950.018.71309314-15
AGAGTAC145500.018.35777310-11
GTAAGGT18200.017.1811494
GTACTAG7050.016.8351171
Produced by FastQC (version 0.11.5)