##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3711576 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1121714872604 33.0 31.0 34.0 30.0 34.0 2 32.211062093299454 34.0 31.0 34.0 30.0 34.0 3 32.27862368977491 34.0 31.0 34.0 30.0 34.0 4 35.708160630416835 37.0 35.0 37.0 35.0 37.0 5 35.751620605370874 37.0 35.0 37.0 35.0 37.0 6 35.833507113959136 37.0 37.0 37.0 35.0 37.0 7 35.75594868594904 37.0 36.0 37.0 35.0 37.0 8 35.738768113599185 37.0 36.0 37.0 35.0 37.0 9 37.43396794245894 39.0 38.0 39.0 35.0 39.0 10-11 37.34804554722845 39.0 37.5 39.0 34.5 39.0 12-13 37.25933242374668 39.0 37.0 39.0 34.0 39.0 14-15 38.601094117431515 40.0 38.0 41.0 34.0 41.0 16-17 38.533315093103305 40.0 38.0 41.0 34.0 41.0 18-19 38.45102956264401 40.0 38.0 41.0 34.0 41.0 20-21 38.33614386449314 40.0 38.0 41.0 34.0 41.0 22-23 38.26087637704307 40.0 38.0 41.0 34.0 41.0 24-25 38.298155419692335 40.0 38.0 41.0 34.0 41.0 26-27 38.22021319245518 40.0 38.0 41.0 34.0 41.0 28-29 38.104779613835206 40.0 38.0 41.0 33.0 41.0 30-31 37.949851087516464 40.0 38.0 41.0 33.0 41.0 32-33 37.76482564279972 40.0 38.0 41.0 33.0 41.0 34-35 37.46373238753564 40.0 37.5 41.0 31.5 41.0 36-37 37.21885110260439 40.0 37.0 41.0 31.0 41.0 38-39 36.979407130555856 40.0 37.0 41.0 30.5 41.0 40-41 36.83407883874666 40.0 37.0 41.0 30.5 41.0 42-43 36.64875756282507 40.0 36.0 41.0 30.0 41.0 44-45 36.411183146997395 39.5 36.0 41.0 30.0 41.0 46-47 36.09998555869528 39.0 35.5 41.0 29.0 41.0 48-49 36.1142433834037 39.0 36.0 41.0 29.0 41.0 50-51 35.11869391870192 38.0 34.5 40.0 27.0 40.5 52-53 35.27323406552904 38.5 35.0 40.0 27.5 40.5 54-55 35.77527363039313 39.0 35.0 41.0 28.0 41.0 56-57 35.645084460078415 39.0 35.0 41.0 28.0 41.0 58-59 35.443086979762775 39.0 35.0 41.0 28.0 41.0 60-61 35.14575937553212 38.5 35.0 40.5 27.0 41.0 62-63 34.776904743429746 38.0 34.5 40.0 26.0 41.0 64-65 34.404567224273464 37.0 34.0 40.0 26.0 41.0 66-67 34.02059260001681 37.0 34.0 39.5 25.5 41.0 68-69 33.618596386009614 36.0 34.0 39.0 24.5 41.0 70-71 33.21018402964131 35.5 33.5 39.0 23.5 40.5 72-73 32.772216438515606 35.0 33.0 38.5 22.5 40.0 74-75 32.33581664500471 35.0 33.0 37.0 21.5 39.5 76-77 31.912956248235254 35.0 32.0 37.0 20.0 39.0 78-79 31.503264246778187 35.0 32.0 36.0 19.0 39.0 80-81 31.105793873007045 35.0 31.5 36.0 18.0 37.5 82-83 30.696175829351198 35.0 31.0 35.0 14.0 37.0 84-85 30.340923774698403 34.5 31.0 35.0 8.5 37.0 86-87 29.983547285573568 34.0 31.0 35.0 4.5 36.0 88-89 29.60378919898178 34.0 30.0 35.0 2.0 36.0 90-91 29.248558967942458 34.0 30.0 35.0 2.0 35.5 92-93 28.867219612369517 34.0 29.5 35.0 2.0 35.0 94-95 28.43304892584713 34.0 29.0 35.0 2.0 35.0 96-97 27.895307950046018 34.0 29.0 35.0 2.0 35.0 98-99 27.22590174632016 34.0 27.0 35.0 2.0 35.0 100-101 24.704657940454403 31.5 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25113627624649126 1101 2 0.278991553588412 1101 3 0.23575838899462553 1101 4 0.17408087842532893 1101 5 0.2758425331963963 1101 6 0.2512735376855062 1101 7 0.1451151995651827 1101 8 0.23678999149363733 1101 9 0.3165177002783395 1101 10-11 0.2850909864382132 1101 12-13 0.1997921009187209 1101 14-15 0.28361424265379753 1101 16-17 0.429048955816846 1101 18-19 0.4147471115983592 1101 20-21 0.355977584303929 1101 22-23 0.3078142575882552 1101 24-25 0.27170226868079794 1101 26-27 0.38569149456198204 1101 28-29 0.3150552123885859 1101 30-31 0.2712543055287 1101 32-33 0.3025412669295875 1101 34-35 0.26726123466733753 1101 36-37 0.2130231704836234 1101 38-39 0.26033131059578096 1101 40-41 0.14017621163810645 1101 42-43 0.010386880717824454 1101 44-45 -0.11353838705932873 1101 46-47 -0.00796431155905708 1101 48-49 0.10712983905037277 1101 50-51 0.019861926864599866 1101 52-53 0.05366795411362091 1101 54-55 0.06685479260772098 1101 56-57 0.11463579451003625 1101 58-59 0.03684546316402759 1101 60-61 -0.08187824101985797 1101 62-63 0.04232592175043237 1101 64-65 0.06977034344317445 1101 66-67 0.055953146413656896 1101 68-69 -0.0131693914830322 1101 70-71 -0.03420982052148247 1101 72-73 -0.07970166750982344 1101 74-75 -0.2528204396889464 1101 76-77 -0.3014942096112385 1101 78-79 -0.23613299086091288 1101 80-81 -0.2780270126137161 1101 82-83 -0.33503690311006196 1101 84-85 -0.4078369774264736 1101 86-87 -0.4307400223884237 1101 88-89 -0.32079565461603465 1101 90-91 -0.5719668408362253 1101 92-93 -0.5477841312233132 1101 94-95 -0.5812956673541976 1101 96-97 -0.8013466974908816 1101 98-99 -0.7961704696627514 1101 100-101 -0.8221599625405389 1102 1 0.2173373399746268 1102 2 0.2905483521617427 1102 3 0.39577297815105794 1102 4 0.39262789588132563 1102 5 0.34603731712921615 1102 6 0.3431152610111994 1102 7 0.36981983807095986 1102 8 0.36652849323296977 1102 9 0.4011297091594841 1102 10-11 0.30849019190890203 1102 12-13 0.439634971964999 1102 14-15 0.4617822385085617 1102 16-17 0.5654976953069308 1102 18-19 0.5544122728967125 1102 20-21 0.6320480065359462 1102 22-23 0.508833138139579 1102 24-25 0.5085013575288784 1102 26-27 0.5428901744755876 1102 28-29 0.5397316205178768 1102 30-31 0.5769575119329744 1102 32-33 0.5736932777821337 1102 34-35 0.466604090040569 1102 36-37 0.5112432499667747 1102 38-39 0.5947524575837022 1102 40-41 0.38427271118472106 1102 42-43 0.3441075083801195 1102 44-45 0.4231923068565422 1102 46-47 0.4422753539515796 1102 48-49 0.4632000969985981 1102 50-51 0.548404348150946 1102 52-53 0.4786006921731101 1102 54-55 0.4779495949327668 1102 56-57 0.4941513801326778 1102 58-59 0.446140366551937 1102 60-61 0.5611912298767905 1102 62-63 0.5600254708575036 1102 64-65 0.48875138169410803 1102 66-67 0.538351270632603 1102 68-69 0.41380107935432875 1102 70-71 0.3668373281727213 1102 72-73 0.2936626229333541 1102 74-75 0.22094948066551723 1102 76-77 0.3230459373830996 1102 78-79 0.3042851661619146 1102 80-81 0.32987982487788514 1102 82-83 0.17153591218925257 1102 84-85 0.14126348381444487 1102 86-87 0.06449050743248463 1102 88-89 0.05090002230307178 1102 90-91 -0.08825713710222516 1102 92-93 -0.10012070000327356 1102 94-95 -0.26959152321046176 1102 96-97 -0.18465062968963508 1102 98-99 -0.45039490474364996 1102 100-101 -0.6505354658248912 1103 1 0.24000119819248056 1103 2 0.2029626597994607 1103 3 0.39616799674130476 1103 4 0.2826541685263777 1103 5 0.2742665320235602 1103 6 0.1952134029430752 1103 7 0.22781051691983834 1103 8 0.31118781430132003 1103 9 0.4247187110325825 1103 10-11 0.3943898584745824 1103 12-13 0.3679177144075467 1103 14-15 0.40477107700307613 1103 16-17 0.4532513924326125 1103 18-19 0.48085162590869146 1103 20-21 0.4083861814452092 1103 22-23 0.4385267269556721 1103 24-25 0.45125654183746633 1103 26-27 0.4109528156866915 1103 28-29 0.42488375923774413 1103 30-31 0.4293667022635077 1103 32-33 0.41221409273261855 1103 34-35 0.33764482192954404 1103 36-37 0.21202158834292106 1103 38-39 0.3167463293232373 1103 40-41 0.1956686250278139 1103 42-43 0.1426461419340157 1103 44-45 0.09104678844591518 1103 46-47 0.09645064986602137 1103 48-49 0.16459639344409283 1103 50-51 0.2633538289981061 1103 52-53 0.34265226219496725 1103 54-55 0.3145453955402573 1103 56-57 0.3148065481254818 1103 58-59 0.1812758032052173 1103 60-61 0.2161342598266529 1103 62-63 0.13829166758540623 1103 64-65 -0.019752048759535512 1103 66-67 0.11830128583552835 1103 68-69 -0.002183708889035074 1103 70-71 0.15870920676709943 1103 72-73 -0.041180150470552235 1103 74-75 -0.08422850027168494 1103 76-77 -0.09855303532943793 1103 78-79 -0.1248696522463355 1103 80-81 -0.13467555709635448 1103 82-83 -0.2770890460325859 1103 84-85 -0.09462855829462669 1103 86-87 -0.25435654228909144 1103 88-89 -0.1518958079928936 1103 90-91 -0.30024319676281763 1103 92-93 -0.3435119792068271 1103 94-95 -0.32865268864314245 1103 96-97 -0.3164721040958369 1103 98-99 -0.5897154323008635 1103 100-101 -0.57446628983827 1104 1 0.15270976606095132 1104 2 0.2007710762331385 1104 3 0.2052756462865304 1104 4 0.15591620377489335 1104 5 0.22478925898262503 1104 6 0.2424513265603636 1104 7 0.1517160111405218 1104 8 0.23315310062133676 1104 9 0.2835849413600826 1104 10-11 0.3022617076083165 1104 12-13 0.2702056183180588 1104 14-15 0.3266707636841417 1104 16-17 0.3504533079420682 1104 18-19 0.3260967006035571 1104 20-21 0.29127971341042525 1104 22-23 0.2776450463000941 1104 24-25 0.29284627380916817 1104 26-27 0.36304469138648443 1104 28-29 0.29666173708047694 1104 30-31 0.3063290231572324 1104 32-33 0.15899070391620995 1104 34-35 0.20958437921084538 1104 36-37 0.22738669066132644 1104 38-39 0.33192139880777205 1104 40-41 0.29342826293213875 1104 42-43 0.20453182864842745 1104 44-45 0.17683122122143402 1104 46-47 0.284087170152894 1104 48-49 0.3738228571987747 1104 50-51 0.3755628627853298 1104 52-53 0.3956776960353139 1104 54-55 0.35796568005986984 1104 56-57 0.3299146737161749 1104 58-59 0.33274299469288593 1104 60-61 0.46374959644868596 1104 62-63 0.48955093868738686 1104 64-65 0.5103572893490664 1104 66-67 0.4072510266542224 1104 68-69 0.4420163151641674 1104 70-71 0.37216241119296 1104 72-73 0.37459717209272014 1104 74-75 0.39847797414382313 1104 76-77 0.366201676687254 1104 78-79 0.4615167378718503 1104 80-81 0.4196188981748179 1104 82-83 0.29208890983606395 1104 84-85 0.40853223305209596 1104 86-87 0.27939258999437655 1104 88-89 0.37335297326080763 1104 90-91 0.2687142564943059 1104 92-93 0.25194629431463156 1104 94-95 0.25615227520625794 1104 96-97 0.13842693429590724 1104 98-99 0.2653261239950062 1104 100-101 0.10417685153128176 1105 1 -0.008643157240669552 1105 2 0.06360645006687093 1105 3 0.13307113782651925 1105 4 0.18199666121549996 1105 5 0.19138135746024432 1105 6 0.1498433553074321 1105 7 0.05653655473226138 1105 8 0.11128432881823613 1105 9 0.16744821914272734 1105 10-11 0.19700347297787602 1105 12-13 0.2291384447007303 1105 14-15 0.25930132886718127 1105 16-17 0.19928666126813965 1105 18-19 0.13378419370563677 1105 20-21 0.14735891306403204 1105 22-23 0.18644748323885096 1105 24-25 0.12206615561144218 1105 26-27 0.12332037877478541 1105 28-29 0.09442915231449689 1105 30-31 0.1304840647125758 1105 32-33 0.14732286722508547 1105 34-35 0.008710829443963064 1105 36-37 0.09941491201094266 1105 38-39 0.1628300458836165 1105 40-41 0.20122301469816506 1105 42-43 0.1049736637913341 1105 44-45 0.023072968225065438 1105 46-47 0.03756186025052699 1105 48-49 -0.0030013941675264277 1105 50-51 -0.05718055217744222 1105 52-53 -0.04655070866522948 1105 54-55 -0.048577877600500585 1105 56-57 -0.07522780854915823 1105 58-59 -0.01994895631797533 1105 60-61 -0.03566958679802923 1105 62-63 0.02013982188811525 1105 64-65 -0.046539848590434474 1105 66-67 0.03559359132215434 1105 68-69 -0.036968110453145187 1105 70-71 -0.22857057295678374 1105 72-73 -0.05546798709610101 1105 74-75 0.005202528123909644 1105 76-77 0.042895534328536655 1105 78-79 -0.1147751930349763 1105 80-81 -0.308881056830824 1105 82-83 -0.30609699142483393 1105 84-85 -0.17521099414165064 1105 86-87 -0.21842297904550279 1105 88-89 -0.24645813093490077 1105 90-91 -0.3515700376161952 1105 92-93 -0.2494275104773429 1105 94-95 -0.3440489964001294 1105 96-97 -0.4521714889066928 1105 98-99 -0.48988091296791936 1105 100-101 -0.5761829115115802 1106 1 0.18008969807455344 1106 2 0.21186148562070883 1106 3 0.2723707718748969 1106 4 0.21618685688557093 1106 5 0.18698299581276245 1106 6 0.25355444195565013 1106 7 0.19600974307338248 1106 8 0.21079044288803317 1106 9 0.2579443860722037 1106 10-11 0.2929046164527236 1106 12-13 0.2829030852449961 1106 14-15 0.3510247725810416 1106 16-17 0.4063319738146234 1106 18-19 0.4572621225582907 1106 20-21 0.526158512401544 1106 22-23 0.47702199753477004 1106 24-25 0.5860164496620044 1106 26-27 0.4569289196294619 1106 28-29 0.4385674800444974 1106 30-31 0.41578252850915476 1106 32-33 0.39681212173501734 1106 34-35 0.42492129625541963 1106 36-37 0.40751345117966764 1106 38-39 0.46768807847215754 1106 40-41 0.49904218104251186 1106 42-43 0.39995893646992187 1106 44-45 0.29109782119978433 1106 46-47 0.39710189178781974 1106 48-49 0.39433380363477966 1106 50-51 0.34253729812950695 1106 52-53 0.2751594457484998 1106 54-55 0.241037129391259 1106 56-57 0.2950527543617909 1106 58-59 0.2893198346532344 1106 60-61 0.3238208577984736 1106 62-63 0.30396059484935023 1106 64-65 0.16214679169598156 1106 66-67 0.21445616607446993 1106 68-69 0.24525844236190153 1106 70-71 0.2713525672849997 1106 72-73 0.23025692439121315 1106 74-75 0.2088313004504272 1106 76-77 0.25741607819810497 1106 78-79 0.23739126040785052 1106 80-81 0.3655881149680873 1106 82-83 0.4108905027487033 1106 84-85 0.4417050948371575 1106 86-87 0.30087377896030887 1106 88-89 0.3631959505661939 1106 90-91 0.45519709219249904 1106 92-93 0.473686450229966 1106 94-95 0.3614244101235293 1106 96-97 0.48956633501999747 1106 98-99 0.4697949867871216 1106 100-101 0.26920748647320636 1107 1 0.0580582994916341 1107 2 0.08521840253608559 1107 3 0.07526962113279012 1107 4 0.13998323043169592 1107 5 0.14535869462766016 1107 6 0.11926566315692355 1107 7 0.032964012181430746 1107 8 0.05829336418590003 1107 9 0.2001425063459692 1107 10-11 0.12781311485075264 1107 12-13 0.23766153224008946 1107 14-15 0.32769036698168463 1107 16-17 0.2633438098131009 1107 18-19 0.18450327612842443 1107 20-21 0.14488077888348272 1107 22-23 0.14734502864898502 1107 24-25 0.19791806956752822 1107 26-27 0.10854584663416489 1107 28-29 0.047212405420111736 1107 30-31 0.15108401867675525 1107 32-33 0.049852779742664666 1107 34-35 0.04235598130235729 1107 36-37 0.03366743046235854 1107 38-39 0.17314443896924558 1107 40-41 -0.014488671844780754 1107 42-43 0.04260433589998769 1107 44-45 0.12972162732405934 1107 46-47 0.16101634627874972 1107 48-49 0.25374091576841806 1107 50-51 0.11461884552851842 1107 52-53 0.19525101475900897 1107 54-55 0.1753220361182244 1107 56-57 0.15291785702499539 1107 58-59 0.1331355873698712 1107 60-61 -0.03663038121745643 1107 62-63 0.01232210651871668 1107 64-65 0.06914621198256299 1107 66-67 0.01016573811804733 1107 68-69 0.05594971847447994 1107 70-71 -0.08807194565697074 1107 72-73 0.05624210015661646 1107 74-75 -0.009894900808887996 1107 76-77 0.015810008670747777 1107 78-79 0.05127307662584357 1107 80-81 0.04138252457433467 1107 82-83 0.05934142322011127 1107 84-85 -0.09500888635178839 1107 86-87 -0.1344573709634851 1107 88-89 -0.1012727745377191 1107 90-91 -0.055655525469102685 1107 92-93 -0.1086482177923962 1107 94-95 -0.18277191865079345 1107 96-97 -0.25019242707082157 1107 98-99 -0.1596662434280809 1107 100-101 -0.07347172696008997 1108 1 -9.749910800564976E-4 1108 2 0.08736767392491629 1108 3 0.15269071925412447 1108 4 0.07500262437768868 1108 5 0.14489947574431739 1108 6 0.1767378816997507 1108 7 0.20828371138625812 1108 8 0.22717386808373874 1108 9 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#Quality Count 2 11766.0 3 3050.0 4 3092.0 5 3478.0 6 4005.0 7 4756.0 8 5745.0 9 7080.0 10 8481.0 11 10257.0 12 13465.0 13 20044.0 14 17367.0 15 17247.0 16 19912.0 17 25031.0 18 24108.0 19 18702.0 20 18416.0 21 19403.0 22 21293.0 23 23717.0 24 26476.0 25 30078.0 26 34491.0 27 40520.0 28 47941.0 29 58104.0 30 69350.0 31 85738.0 32 107949.0 33 142189.0 34 199168.0 35 302194.0 36 502421.0 37 818679.0 38 795218.0 39 150589.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81619175092126 18.14681615903944 13.720547163062951 24.316444926976345 2 15.631253064996539 19.619970101279087 39.77233920535868 24.976437628365698 3 19.87625822746843 23.017032430831545 29.035485264867706 28.071224076832323 4 13.250612877329582 15.547823853876233 36.01811548853229 35.18344778026189 5 14.332605503215879 36.61049343428525 34.02402999527041 15.032871067228463 6 33.77846343687793 34.688570724369896 16.888193521977676 14.644772316774505 7 29.635947289204147 31.695490326744256 20.248208808667297 18.420353575384297 8 26.670818298501608 34.68785491260402 19.62700581668972 19.014320972204654 9 26.28135549981784 16.882290012973804 20.050719465104887 36.78563502210348 10-11 25.569698148869502 25.917094389439477 26.874729576330296 21.638477885360725 12-13 26.245243765808883 23.02462987366104 27.42599124983702 23.304135110693053 14-15 22.775302858156856 24.69904309200777 25.423971754723258 27.101682295112116 16-17 22.82685970675915 27.799488286639296 26.397568411023098 22.97608359557845 18-19 22.824658757838616 26.646797507176522 28.12568434520233 22.40285938978253 20-21 23.215651411895806 25.827510643482803 28.334381419754095 22.622456524867292 22-23 22.86188800130339 25.81131262174629 28.20556809180717 23.121231285143146 24-25 22.866605499092604 25.97268987419461 28.35332879927117 22.807375827441614 26-27 22.483563946163663 26.206852829537148 28.241645736607683 23.067937487691506 28-29 22.511789977884856 26.819913449342074 28.038404494030424 22.629892078742646 30-31 22.69193109847545 26.267249657434483 27.910404281259776 23.130414962830294 32-33 22.350967633700243 26.233248655354423 28.090581027952094 23.32520268299324 34-35 23.05843016820032 26.342854764053797 27.88229194458794 22.71642312315795 36-37 23.184933018278127 25.929603736041358 27.94394259258808 22.941520653092436 38-39 22.647979121329325 26.345882091488864 27.981322018955435 23.024816768226373 40-41 23.06041169699337 26.207007322223998 27.972294814201593 22.760286166581036 42-43 22.772254310535615 26.251788259273333 27.7332905960404 23.24266683415065 44-45 22.8076808482499 26.425437750439713 27.859149884727174 22.90773151658322 46-47 23.23434830085291 26.660868863604424 27.256195115928534 22.848587719614127 48-49 22.9640531282579 26.717651200259958 27.278490095993718 23.03980557548843 50-51 22.936054173852405 26.83313349317838 27.44334880204886 22.787463530920352 52-53 23.309929267934084 26.579287036588266 27.23843851714744 22.872345178330207 54-55 22.8384323632062 26.745517499879185 27.344709291472796 23.071340845441817 56-57 22.872482382224263 26.95217893099159 27.20120696244537 22.974131724338786 58-59 22.989815565258453 26.56520568444895 27.244519794498213 23.200458955794385 60-61 22.699530465633753 26.700432313529287 27.62252715416946 22.9775100666675 62-63 22.656487070091472 26.708028045142274 27.768477881601555 22.8670070031647 64-65 22.50713238177143 26.88967499734634 27.505323562096674 23.097869058785555 66-67 22.456374428573326 26.858739507426886 27.33302263284274 23.35186343115705 68-69 22.816295152158514 26.49399695551617 27.57849783526393 23.111210057061392 70-71 22.802129094030114 26.51692668751311 27.29340769735006 23.387536521106718 72-73 22.70990867743127 26.61685681821382 27.42722140442434 23.24601309993057 74-75 22.60912577111817 27.012449329518684 27.191658687889603 23.186766211473543 76-77 22.776650780403376 26.67572325817987 27.20899269448489 23.338633266931865 78-79 22.791694142079557 26.78356033708948 27.335017589678124 23.089727931152844 80-81 22.9331394062241 26.686349023048876 27.16706574126296 23.213445829464064 82-83 22.818894244019607 26.768655104132655 27.315417199337027 23.09703345251071 84-85 22.708685625608812 26.91661888216206 27.188381407523043 23.18631408470609 86-87 22.853045348980135 26.858193264228785 27.134947180667623 23.153814206123457 88-89 22.766183169893228 26.87074747041507 26.901114945292516 23.46195441439919 90-91 22.655846631004604 26.747929774484042 27.133099031626667 23.463124562884687 92-93 22.883360756154968 26.558849303019834 27.24539020382432 23.312399737000877 94-95 22.69560464096965 26.369148197350214 27.516608404272997 23.418638757407145 96-97 22.860688898403286 26.036227651206488 27.547283806286487 23.555799644103733 98-99 22.61044815626944 26.30447601143019 27.246322456691672 23.838753375608693 100-101 22.858169031493127 25.70118642271154 27.735001710516215 23.705642835279118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3157.0 1 1779.5 2 429.0 3 447.5 4 398.5 5 389.5 6 627.5 7 1187.0 8 1802.5 9 2177.5 10 2217.0 11 2177.0 12 2443.5 13 2825.0 14 3137.0 15 3429.5 16 3737.0 17 4078.5 18 4347.5 19 4742.5 20 5278.5 21 5991.5 22 7217.0 23 8866.5 24 11131.5 25 14202.5 26 18618.5 27 24086.0 28 28420.0 29 32767.0 30 38269.0 31 46158.0 32 57709.5 33 62786.0 34 63831.5 35 68794.5 36 76035.5 37 83741.5 38 89872.5 39 94873.0 40 100255.5 41 107508.5 42 111749.0 43 115490.0 44 121266.5 45 128609.5 46 134074.0 47 140440.5 48 149615.0 49 148738.0 50 141042.0 51 136549.5 52 135517.5 53 136779.5 54 136856.0 55 128510.5 56 117945.0 57 108485.5 58 100064.0 59 89664.5 60 73222.5 61 60771.0 62 51269.0 63 38377.0 64 27169.5 65 20636.0 66 15673.0 67 12678.5 68 10153.5 69 7989.0 70 6340.0 71 4162.5 72 2871.5 73 2270.5 74 1707.0 75 1329.5 76 984.5 77 641.0 78 439.5 79 300.0 80 195.5 81 139.5 82 100.0 83 73.0 84 63.5 85 50.0 86 29.5 87 27.0 88 24.5 89 18.5 90 20.5 91 18.5 92 16.0 93 13.5 94 11.5 95 8.0 96 4.0 97 5.5 98 5.5 99 3.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3386432070904651 2 0.11838636740834621 3 0.11256673714885536 4 0.12048790055760679 5 0.13808150499949348 6 0.0868094847040718 7 0.09615861294501311 8 0.1023015559966979 9 0.08985401349723136 10-11 0.08822397816992027 12-13 0.09204984621088184 14-15 0.09394930886502122 16-17 0.08229657697969811 18-19 0.09839485975768783 20-21 0.1084579704147241 22-23 0.11659467568493816 24-25 0.1298370287985481 26-27 0.12894791862001478 28-29 0.12431376859856837 30-31 0.11466827029811594 32-33 0.1077844020976534 34-35 0.3296982198397662 36-37 0.49183958512502507 38-39 0.46555694939292636 40-41 0.44335613766227605 42-43 0.4766304125255687 44-45 0.4835546948250554 46-47 0.4909774176791746 48-49 0.5018353389503543 50-51 0.4905598053225907 52-53 0.4942105456011139 54-55 0.48094124975482117 56-57 0.45834976840026986 58-59 0.4373074941749812 60-61 0.3966509105565937 62-63 0.46850717862169605 64-65 0.5000571185932876 66-67 0.5077357974078935 68-69 0.5206548377293096 70-71 0.512437304261047 72-73 0.504691268614734 74-75 0.51103628216154 76-77 0.4885660431040615 78-79 0.4678336103046253 80-81 0.4689247909782798 82-83 0.4828676551416433 84-85 0.48254434234944943 86-87 0.48610078306358273 88-89 0.4979016999786614 90-91 0.5130839298454349 92-93 0.5062000616449723 94-95 0.5103627138444693 96-97 0.5099451014878855 98-99 0.50446225538693 100-101 0.491610571897221 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3711576.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.926403705229774 #Duplication Level Percentage of deduplicated Percentage of total 1 72.14409117322573 35.297509296881465 2 14.52352182850486 14.211673844062913 3 5.3928178196307055 7.915535452560267 4 2.58443957952133 5.057893368777395 5 1.4195147480094044 3.4725875813317812 6 0.8861799884085833 2.6014559921024514 7 0.5820063409145371 1.9932834036211762 8 0.4328918630260051 1.6943873640895488 9 0.3007167350923332 1.3241689543841517 >10 1.5616687995293743 13.76976680127199 >50 0.11011797006934985 3.6741982640735147 >100 0.05563511025956752 4.9607983888118445 >500 0.003585110772943641 1.1865310407193501 >1k 0.002702621935723377 2.47941241544163 >5k 1.1031109941728065E-4 0.36079783187077125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7432 0.20023838929877766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5938 0.15998594667063262 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4223 0.11377916011958261 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4182 0.11267450807958668 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3921 0.10564245484936856 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3873 0.10434920368059283 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.2901322780403797E-4 14-15 0.0 0.0 0.0 0.0 3.6372689121817796E-4 16-17 0.0 0.0 0.0 0.0 4.3108372292524795E-4 18-19 0.0 0.0 0.0 2.6942732682827997E-5 4.5802645560807595E-4 20-21 0.0 0.0 0.0 6.735683170706999E-5 4.8496918829090394E-4 22-23 0.0 0.0 0.0 1.8859912877979598E-4 5.388546536565599E-4 24-25 0.0 0.0 0.0 2.8289869316969397E-4 5.927401190222159E-4 26-27 0.0 0.0 0.0 5.25383287315146E-4 6.33154218046458E-4 28-29 0.0 0.0 0.0 0.0022227754463333095 6.466255843878719E-4 30-31 0.0 0.0 0.0 0.0080828198048484 6.466255843878719E-4 32-33 0.0 0.0 0.0 0.01938529616529474 6.735683170706999E-4 34-35 0.0 0.0 0.0 0.03687112967645011 7.005110497535279E-4 36-37 0.0 0.0 0.0 0.0669796334495104 7.005110497535279E-4 38-39 0.0 0.0 0.0 0.11594805010055029 7.139824160949419E-4 40-41 0.0 0.0 0.0 0.16829777970328508 7.274537824363559E-4 42-43 0.0 0.0 0.0 0.2239614654260077 7.4092514877777E-4 44-45 0.0 0.0 0.0 0.2909680416082009 7.813392478020119E-4 46-47 0.0 0.0 0.0 0.3723620370430243 8.082819804848399E-4 48-49 0.0 0.0 0.0 0.4626336628968395 8.082819804848399E-4 50-51 0.0 0.0 0.0 0.5628471571106183 8.352247131676679E-4 52-53 2.6942732682827997E-5 0.0 0.0 0.6595985101746535 9.295242775575658E-4 54-55 2.6942732682827997E-5 0.0 0.0 0.7715455644718039 9.968811092646358E-4 56-57 2.6942732682827997E-5 0.0 0.0 0.9042789370337561 0.001064237940971706 58-59 2.6942732682827997E-5 0.0 0.0 1.0538784602551585 0.0011046520399959478 60-61 2.6942732682827997E-5 0.0 0.0 1.2220415262950293 0.0011046520399959478 62-63 2.6942732682827997E-5 0.0 0.0 1.395471896574393 0.0011585375053616038 64-65 2.6942732682827997E-5 0.0 0.0 1.5773757562825064 0.0011854802380444319 66-67 2.6942732682827997E-5 0.0 0.0 1.7939414415870778 0.0012124229707272598 68-69 2.6942732682827997E-5 0.0 0.0 2.035523454187655 0.0012393657034100879 70-71 2.6942732682827997E-5 0.0 0.0 2.2963425779237716 0.001266308436092916 72-73 2.6942732682827997E-5 0.0 0.0 2.5726133588534896 0.0013606080004828138 74-75 2.6942732682827997E-5 0.0 0.0 2.855296510161721 0.0014683789312141258 76-77 2.6942732682827997E-5 0.0 0.0 3.169071574985936 0.0015492071292626097 78-79 2.6942732682827997E-5 0.0 0.0 3.5289052413314455 0.0015761498619454378 80-81 2.6942732682827997E-5 0.0 0.0 3.921784169312443 0.0016300353273110938 82-83 2.6942732682827997E-5 0.0 0.0 4.328592490090463 0.0017647489907252336 84-85 2.6942732682827997E-5 0.0 0.0 4.753102186241101 0.0018051630897494757 86-87 2.6942732682827997E-5 0.0 0.0 5.196646923032157 0.0018455771887737177 88-89 2.6942732682827997E-5 0.0 0.0 5.680740472510869 0.0018859912877979598 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10080 0.0 28.21185 1 GTATCAA 13945 0.0 26.350428 1 TACATGG 12215 0.0 23.660067 2 GTACATG 12805 0.0 23.505793 1 ACATGGG 12225 0.0 22.788097 3 TCAACGC 16065 0.0 22.472628 4 ATCAACG 16155 0.0 22.288778 3 TATCAAC 16380 0.0 22.15616 2 CAACGCA 16290 0.0 22.07498 5 AACGCAG 16725 0.0 21.6343 6 GAGTACT 9120 0.0 20.174784 12-13 CATGGGG 7740 0.0 19.649149 4 TAAGGTG 1885 0.0 19.60482 5 GAGTACA 9190 0.0 19.268974 1 ACGCAGA 18835 0.0 19.034693 7 CGCAGAG 18895 0.0 18.97476 8 GTACTTT 9795 0.0 18.713093 14-15 AGAGTAC 14550 0.0 18.357773 10-11 GTAAGGT 1820 0.0 17.181149 4 GTACTAG 705 0.0 16.835117 1 >>END_MODULE