FastQCFastQC Report
Fri 27 May 2016
SRR939171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711576
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76890.20716267159826446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64840.17469667871545672No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46800.126091988955635No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA41770.11253979441617254No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40130.10812118625618874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.10475334467083525No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38250.10305595251181708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153600.025.26151
TAAGGTG18400.024.7550285
GGTATCA111700.024.5505961
GTAAGGT18900.022.3428334
GTACATG116350.022.0963291
TACATGG113050.022.0762462
TCAACGC175750.021.6245574
ATCAACG176700.021.5082973
CAACGCA177650.021.5001135
TATCAAC180300.021.1577932
AACGCAG182750.020.9260716
ACATGGG112800.020.7791563
GAGTACT103700.019.88208812-13
GGTAAGG20350.019.1187483
ACGCAGA204050.018.6254147
CGCAGAG205050.018.557728
GTACTTT110150.018.52400614-15
AGAGTAC155550.018.47119910-11
GTGTAGC23250.018.4294451
AGGTAAG19950.017.8372692
Produced by FastQC (version 0.11.5)