##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939171_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3711576 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.526802899900204 34.0 31.0 34.0 31.0 34.0 2 32.73254003151222 34.0 31.0 34.0 31.0 34.0 3 32.82938460643134 34.0 31.0 34.0 31.0 34.0 4 36.27760498505217 37.0 37.0 37.0 35.0 37.0 5 36.235830547454775 37.0 37.0 37.0 35.0 37.0 6 36.27039726520486 37.0 37.0 37.0 35.0 37.0 7 36.22282044069689 37.0 37.0 37.0 35.0 37.0 8 36.20465160891222 37.0 37.0 37.0 35.0 37.0 9 37.96137031816134 39.0 38.0 39.0 35.0 39.0 10-11 37.90326575557122 39.0 38.0 39.0 35.0 39.0 12-13 37.83994952548459 39.0 38.0 39.0 35.0 39.0 14-15 39.237312128325 41.0 39.0 41.0 36.0 41.0 16-17 39.179652659678794 40.0 39.0 41.0 36.0 41.0 18-19 39.188475865777775 40.5 38.5 41.0 36.0 41.0 20-21 39.24272748288059 41.0 39.0 41.0 36.0 41.0 22-23 39.20526725035403 41.0 39.0 41.0 36.0 41.0 24-25 39.15214749745122 41.0 39.0 41.0 36.0 41.0 26-27 39.02351117153468 40.0 39.0 41.0 35.0 41.0 28-29 38.883042675133154 40.0 38.0 41.0 35.0 41.0 30-31 38.708612729471255 40.0 38.0 41.0 35.0 41.0 32-33 38.58374299758377 40.0 38.0 41.0 34.5 41.0 34-35 38.54666440886567 40.0 38.0 41.0 34.0 41.0 36-37 38.45097298290537 40.0 38.0 41.0 34.0 41.0 38-39 38.32167426990583 40.0 38.0 41.0 34.0 41.0 40-41 38.161216960126914 40.0 38.0 41.0 33.5 41.0 42-43 38.01050591446868 40.0 38.0 41.0 33.0 41.0 44-45 37.82367436366654 40.0 37.5 41.0 33.0 41.0 46-47 37.58252855390809 40.0 37.0 41.0 33.0 41.0 48-49 37.29875732034047 40.0 37.0 41.0 32.0 41.0 50-51 37.05311193951033 40.0 36.5 41.0 31.5 41.0 52-53 36.81792074310212 39.5 36.0 41.0 31.0 41.0 54-55 36.56628949535184 39.0 35.5 41.0 31.0 41.0 56-57 36.28413320918122 39.0 35.0 40.5 30.5 41.0 58-59 35.97419910032827 38.5 35.0 40.0 30.0 41.0 60-61 35.61267302622929 38.0 35.0 40.0 29.0 41.0 62-63 35.220178571043675 37.5 34.5 40.0 28.0 41.0 64-65 34.810547460162475 37.0 34.0 40.0 28.0 41.0 66-67 34.453621722955425 36.5 34.0 39.0 28.0 41.0 68-69 33.989867377092644 36.0 33.5 39.0 26.5 40.0 70-71 33.55206076879471 35.5 33.0 38.5 26.0 40.0 72-73 33.096163058495904 35.0 33.0 37.5 26.0 40.0 74-75 32.61644002439934 35.0 32.5 37.0 25.0 39.0 76-77 30.644887778129828 33.5 29.5 35.0 21.5 37.5 78-79 31.62598920243045 34.5 31.5 36.0 23.0 38.0 80-81 31.677259336734586 35.0 32.0 36.0 24.0 37.0 82-83 31.400428820533378 35.0 32.0 35.0 23.0 37.0 84-85 31.043436130635612 34.5 31.5 35.0 21.0 36.5 86-87 30.514584101201216 34.0 31.0 35.0 19.0 36.0 88-89 29.97976439119123 34.0 30.5 35.0 14.0 36.0 90-91 29.546549363397112 34.0 30.0 35.0 8.5 35.0 92-93 29.28353467637467 34.0 30.0 35.0 4.5 35.0 94-95 28.97687505253833 34.0 29.5 35.0 2.0 35.0 96-97 28.446136223534154 34.0 29.0 35.0 2.0 35.0 98-99 27.69560922368288 34.0 29.0 35.0 2.0 35.0 100-101 24.06450642530289 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10480812301919684 1101 2 0.12115600799624104 1101 3 0.17589783081782429 1101 4 0.1028401913953374 1101 5 0.12524551119525995 1101 6 0.09189800540691095 1101 7 0.15304407169428202 1101 8 0.1417365689322878 1101 9 0.21240716203880083 1101 10-11 0.22657373237245793 1101 12-13 0.18806740065883787 1101 14-15 0.25671585382881545 1101 16-17 0.2527065498634897 1101 18-19 0.265110958175768 1101 20-21 0.2669557486299041 1101 22-23 0.22829397629210746 1101 24-25 0.1832636212640395 1101 26-27 0.15610739612714752 1101 28-29 0.11678797776880856 1101 30-31 0.17192426071259348 1101 32-33 0.23701597081329595 1101 34-35 0.23915572891977632 1101 36-37 0.3056691750253293 1101 38-39 0.24549954332210433 1101 40-41 0.2820694358895395 1101 42-43 0.1830765699942276 1101 44-45 0.2501983192921742 1101 46-47 0.2919221043998661 1101 48-49 0.24608384435654784 1101 50-51 0.2463306078610188 1101 52-53 0.22434963995632984 1101 54-55 0.15737066610322614 1101 56-57 0.21573337915530288 1101 58-59 0.2790569628264379 1101 60-61 0.2641832450849009 1101 62-63 0.22693298912609805 1101 64-65 0.13622172128386723 1101 66-67 0.2574312060166122 1101 68-69 0.18695609522294632 1101 70-71 0.14851887336344305 1101 72-73 0.15812227203336704 1101 74-75 0.23931125159536748 1101 76-77 0.2232256079012771 1101 78-79 0.31902210975145806 1101 80-81 0.31540692122326064 1101 82-83 0.48490841118326955 1101 84-85 0.4236086440961344 1101 86-87 0.3773332612273599 1101 88-89 0.5186894339535648 1101 90-91 0.38223236003423366 1101 92-93 0.3352652951516255 1101 94-95 0.2388154981014381 1101 96-97 0.1961272120321773 1101 98-99 0.2878140606302573 1101 100-101 -0.07972350000995476 1102 1 0.09562484763838341 1102 2 0.08410198657439594 1102 3 0.1340522930867465 1102 4 0.10418070940669821 1102 5 0.12860086611057397 1102 6 0.14630483326808275 1102 7 0.14895491582219478 1102 8 0.11717878503383616 1102 9 0.24560938934941845 1102 10-11 0.18635819203171877 1102 12-13 0.2039581757271307 1102 14-15 0.2446437800725647 1102 16-17 0.2554966363371989 1102 18-19 0.25167032486362473 1102 20-21 0.23946568779958 1102 22-23 0.28303116564939046 1102 24-25 0.2573676042208177 1102 26-27 0.26668195698901087 1102 28-29 0.19518680179895398 1102 30-31 0.23212983450302005 1102 32-33 0.2742119466533879 1102 34-35 0.3048657068377665 1102 36-37 0.28111960531669666 1102 38-39 0.33339652000928766 1102 40-41 0.409438002243391 1102 42-43 0.35391377910332267 1102 44-45 0.36539038782155586 1102 46-47 0.35420001442295046 1102 48-49 0.24352040343054426 1102 50-51 0.29701975950327864 1102 52-53 0.3230249555039535 1102 54-55 0.2963487623523804 1102 56-57 0.23143914612082028 1102 58-59 0.21842340142188021 1102 60-61 0.15533286154832382 1102 62-63 0.24320293874951204 1102 64-65 0.08126742239235085 1102 66-67 0.21510957573219258 1102 68-69 0.10818664128340316 1102 70-71 0.1561750647665221 1102 72-73 0.0019625927153370526 1102 74-75 0.02970594624844125 1102 76-77 0.14787269042876972 1102 78-79 0.11558273406010144 1102 80-81 0.17812326126730582 1102 82-83 0.2045568854022335 1102 84-85 0.28180585791848145 1102 86-87 0.4081449232619825 1102 88-89 0.38385605737771655 1102 90-91 0.5490081243598759 1102 92-93 0.4574963578562965 1102 94-95 0.33361560437605675 1102 96-97 0.37598199648617125 1102 98-99 0.2670001061757681 1102 100-101 0.14595385907703573 1103 1 0.16804835612820312 1103 2 0.1121180477819479 1103 3 0.12796514398419845 1103 4 0.0733506810775637 1103 5 0.13681017096430992 1103 6 0.1519914454764475 1103 7 0.12040330321742942 1103 8 0.1745198491431026 1103 9 0.19794743034783835 1103 10-11 0.26538306357939945 1103 12-13 0.21376751280775608 1103 14-15 0.29198015914177944 1103 16-17 0.2146665215546335 1103 18-19 0.25989802406381557 1103 20-21 0.21459924455064083 1103 22-23 0.2197702490186373 1103 24-25 0.20590661485881867 1103 26-27 0.16519754242678886 1103 28-29 0.21589828408345113 1103 30-31 0.2356195639277061 1103 32-33 0.21043111381991508 1103 34-35 0.37650308694571066 1103 36-37 0.48546428557052934 1103 38-39 0.2959621855268537 1103 40-41 0.39446468232321763 1103 42-43 0.31023796695154715 1103 44-45 0.42301454092284985 1103 46-47 0.342972933088852 1103 48-49 0.3523813188104796 1103 50-51 0.43303335145375854 1103 52-53 0.4905880706453871 1103 54-55 0.4099194121860137 1103 56-57 0.5329497082465267 1103 58-59 0.5028800131133622 1103 60-61 0.49713076727839933 1103 62-63 0.4731956840192666 1103 64-65 0.3804947402777401 1103 66-67 0.3832215321861625 1103 68-69 0.36882823056964753 1103 70-71 0.2628227723802894 1103 72-73 0.2611738836066664 1103 74-75 0.3069538135619325 1103 76-77 0.30500483155093505 1103 78-79 0.40431175461816693 1103 80-81 0.3513033200298885 1103 82-83 0.31165079387527683 1103 84-85 0.289170218467536 1103 86-87 0.5279329081852389 1103 88-89 0.6115948054180258 1103 90-91 0.6340890125787233 1103 92-93 0.5765995212608139 1103 94-95 0.37686034501853527 1103 96-97 0.26675517156926176 1103 98-99 0.25060780038373665 1103 100-101 0.03246235091904737 1104 1 0.03664116680209162 1104 2 0.04276901307346748 1104 3 0.07045191517175908 1104 4 0.016631010739054375 1104 5 0.10631126778668687 1104 6 0.06531388974767083 1104 7 0.056343732292837956 1104 8 0.006139197738690427 1104 9 0.08160503679624753 1104 10-11 0.07230136998312275 1104 12-13 0.09976322660621406 1104 14-15 0.09658966295220495 1104 16-17 0.08725938671565103 1104 18-19 0.08416169948253582 1104 20-21 0.04861598213720697 1104 22-23 0.1179453602646916 1104 24-25 0.05264806929601917 1104 26-27 0.07858670654938038 1104 28-29 0.14829848346333563 1104 30-31 0.14487711581728746 1104 32-33 0.1287776659584594 1104 34-35 0.16825268667141557 1104 36-37 0.2276485187510744 1104 38-39 0.31659918669851095 1104 40-41 0.3787512835611082 1104 42-43 0.36127851882015705 1104 44-45 0.4123356007907617 1104 46-47 0.3917811256592856 1104 48-49 0.22739675533754422 1104 50-51 0.2752475794419027 1104 52-53 0.22015445523958022 1104 54-55 0.3267193542575839 1104 56-57 0.3789360050913544 1104 58-59 0.34829975132779367 1104 60-61 0.32881092100380727 1104 62-63 0.3848181387398313 1104 64-65 0.25712364114862396 1104 66-67 0.34699856761659476 1104 68-69 0.28218289214989056 1104 70-71 0.2784016560357543 1104 72-73 0.25077687853487163 1104 74-75 0.18443492722871468 1104 76-77 0.22427963650402916 1104 78-79 0.3499762995726563 1104 80-81 0.3243138631106568 1104 82-83 0.46640518147845 1104 84-85 0.2638215142665281 1104 86-87 0.300565948644266 1104 88-89 0.30086329595778594 1104 90-91 0.3759879052532291 1104 92-93 0.47839556230468006 1104 94-95 0.40638493224714267 1104 96-97 0.3085984210527535 1104 98-99 0.3004001667724161 1104 100-101 -0.0712914091859389 1105 1 -0.05875088900153003 1105 2 -0.004021433875877278 1105 3 -0.00881769159984458 1105 4 0.010180250188902562 1105 5 0.029672910317799506 1105 6 -0.022485122568454585 1105 7 0.010194165921113552 1105 8 0.0010591595889550831 1105 9 -0.0063843988204297375 1105 10-11 0.054401879408260356 1105 12-13 0.021268841310863706 1105 14-15 0.01251366590342684 1105 16-17 0.06241980593117802 1105 18-19 0.010815336195122427 1105 20-21 0.04446301605884173 1105 22-23 0.0486914283103701 1105 24-25 -0.00257378883296866 1105 26-27 0.0719282698681667 1105 28-29 -0.024748733723598093 1105 30-31 -0.01001858247411036 1105 32-33 0.022217763060638163 1105 34-35 0.15223760761193006 1105 36-37 0.06762590427170068 1105 38-39 0.12489635116370579 1105 40-41 0.09166324198170628 1105 42-43 0.1094086416371951 1105 44-45 0.09930478192690373 1105 46-47 0.1364632938443222 1105 48-49 0.19961224909857123 1105 50-51 0.23007123539289154 1105 52-53 0.19953715153248197 1105 54-55 0.20225208356204405 1105 56-57 0.14774683469734384 1105 58-59 0.15686923652472728 1105 60-61 0.2602429067926124 1105 62-63 0.21834467808831448 1105 64-65 0.1489146587685113 1105 66-67 0.10902736520219491 1105 68-69 -0.07624294931945741 1105 70-71 0.006373580243504762 1105 72-73 -0.13526642067552785 1105 74-75 -0.13822528285510316 1105 76-77 -0.17792678218252433 1105 78-79 -0.12218268367651675 1105 80-81 -0.240364130146272 1105 82-83 -0.16452004201420323 1105 84-85 -0.07058132331464151 1105 86-87 0.10886264218853015 1105 88-89 0.13774497570364375 1105 90-91 0.12067615259939757 1105 92-93 0.011294697976399704 1105 94-95 -0.16320348200196477 1105 96-97 -0.041949298521192446 1105 98-99 -0.0666846263087777 1105 100-101 -0.03905687138241731 1106 1 0.05750569366486502 1106 2 0.10689463526815501 1106 3 0.10923889701697931 1106 4 0.052735762769110295 1106 5 0.08075241801865474 1106 6 0.11601616173331308 1106 7 0.1430375802795112 1106 8 0.07582634890197681 1106 9 0.0991167763900691 1106 10-11 0.1450542452012087 1106 12-13 0.10645672622948865 1106 14-15 0.09016433582299754 1106 16-17 0.04078998236151676 1106 18-19 0.08440235631750426 1106 20-21 0.14328730969287307 1106 22-23 0.16269924321223073 1106 24-25 0.1809053301062633 1106 26-27 0.20231217196371176 1106 28-29 0.16118245943737008 1106 30-31 0.1583340401791844 1106 32-33 0.1545653080112075 1106 34-35 0.05193562314255473 1106 36-37 0.2258032866762676 1106 38-39 0.2805125680768228 1106 40-41 0.17058829627940497 1106 42-43 0.19926265768352636 1106 44-45 0.2041166986605134 1106 46-47 0.19642878133354458 1106 48-49 0.14866414679079298 1106 50-51 0.215579451948976 1106 52-53 0.22992402884447927 1106 54-55 0.15930979284372881 1106 56-57 0.15453528689057094 1106 58-59 0.041802740437141495 1106 60-61 0.21197993732824472 1106 62-63 0.29308707393094835 1106 64-65 0.31532843203718386 1106 66-67 0.2990668364070572 1106 68-69 0.04016487351632492 1106 70-71 0.16138483647906554 1106 72-73 -0.029725575912401325 1106 74-75 0.13383842644641675 1106 76-77 0.1788289426297922 1106 78-79 0.3423874471338344 1106 80-81 0.21028198769213802 1106 82-83 0.1940902343834061 1106 84-85 0.33368612880916615 1106 86-87 0.38781149821780403 1106 88-89 0.47675849310369856 1106 90-91 0.5407494420978765 1106 92-93 0.44699283251569 1106 94-95 0.4257877212747374 1106 96-97 0.48243755895601836 1106 98-99 0.5406636624458976 1106 100-101 0.06452033052568851 1107 1 -0.06423475086151598 1107 2 -0.0476676528848472 1107 3 -0.01034855019665315 1107 4 0.01045947994546026 1107 5 -0.015957825915599244 1107 6 0.04135321225554378 1107 7 0.014431184369421146 1107 8 0.031981641867318444 1107 9 0.05508849866563992 1107 10-11 0.0455546987195703 1107 12-13 0.0412579353191731 1107 14-15 0.1280084289696859 1107 16-17 0.10387669137328004 1107 18-19 0.0730943391223775 1107 20-21 0.08760197964029715 1107 22-23 0.10046486028095103 1107 24-25 0.013525230297737778 1107 26-27 0.139636341498381 1107 28-29 0.10028590081562072 1107 30-31 0.09788888360796477 1107 32-33 0.12398887409838011 1107 34-35 0.13160929998351634 1107 36-37 0.07799102290007198 1107 38-39 0.05813627293152024 1107 40-41 0.04781352262315863 1107 42-43 0.08393676249031756 1107 44-45 0.050180682843652846 1107 46-47 0.07779453785955326 1107 48-49 0.12436963983407878 1107 50-51 0.04681755551795419 1107 52-53 0.03387351069230249 1107 54-55 0.04817851142625784 1107 56-57 0.11688417027600195 1107 58-59 -0.06260595031036331 1107 60-61 -7.011290559901795E-4 1107 62-63 -0.15187581531807837 1107 64-65 -0.0881693499084264 1107 66-67 -0.022016092710202884 1107 68-69 0.09596213309280444 1107 70-71 0.08462179755173338 1107 72-73 7.161976908562906E-4 1107 74-75 0.053958604894049245 1107 76-77 0.08490031980500845 1107 78-79 0.22291263488228807 1107 80-81 0.18110459444609717 1107 82-83 0.16338721350901864 1107 84-85 0.2945999941808388 1107 86-87 0.12593717093148626 1107 88-89 0.20266158721824468 1107 90-91 0.392789976888988 1107 92-93 0.2661090783806479 1107 94-95 0.200492558103754 1107 96-97 0.2641103221761689 1107 98-99 0.44221208356552566 1107 100-101 -0.1805499107373052 1108 1 -0.0028473019774040154 1108 2 0.011596229980924022 1108 3 0.03870757884067899 1108 4 0.052782767620009 1108 5 0.08416897716493565 1108 6 0.038299111825566 1108 7 0.019745089281322237 1108 8 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-0.04522871839453302 2316 74-75 -0.24175898625118464 2316 76-77 -0.2974487069584164 2316 78-79 -0.19858814799681923 2316 80-81 -0.15394235507651288 2316 82-83 -0.31757528296721915 2316 84-85 -0.33235742689021563 2316 86-87 -0.3529827655521096 2316 88-89 -0.5714335463785538 2316 90-91 -0.6398825271605801 2316 92-93 -0.6969762989716095 2316 94-95 -0.29493524808046345 2316 96-97 -0.45990031859611946 2316 98-99 -0.480444170993632 2316 100-101 -0.2034850172230378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 22.0 5 48.0 6 120.0 7 304.0 8 686.0 9 1428.0 10 2452.0 11 3803.0 12 5654.0 13 8045.0 14 9971.0 15 13229.0 16 16947.0 17 23055.0 18 23284.0 19 17604.0 20 16622.0 21 17064.0 22 18146.0 23 20140.0 24 22921.0 25 27240.0 26 32237.0 27 39571.0 28 48346.0 29 58828.0 30 73309.0 31 90920.0 32 113969.0 33 148668.0 34 209317.0 35 314084.0 36 527544.0 37 905579.0 38 817101.0 39 83302.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.155579647160124 18.662339189665964 14.0659584275632 24.116122735610713 2 15.909144794556273 20.061558755633726 39.204047013990824 24.825249435819178 3 19.94155582426441 23.057563687231518 29.264549614503384 27.736330874000693 4 13.566096989526821 15.956564004078052 35.69133435500176 34.78600465139337 5 14.26127337820915 36.71968457603994 34.01296376525767 15.006078280493245 6 33.84904417961535 34.99852892679552 16.61202680478589 14.540400088803246 7 29.61291914809235 31.85609024306656 20.082789628987793 18.4482009798533 8 26.655900350686608 35.07752501902157 19.345878947379767 18.920695682912054 9 26.203585754407293 16.85265235037623 19.911487734590374 37.0322741606261 10-11 25.539865006132167 26.079568355868233 26.766392497418884 21.614174140580715 12-13 26.213891349658475 23.14879178009557 27.389241120214162 23.248075750031795 14-15 22.707037847375695 24.797458619104194 25.422727123927885 27.07277640959223 16-17 22.736388125346558 27.909078319235714 26.382728609603333 22.97180494581439 18-19 22.7443366689797 26.732873070444903 28.185935645370986 22.33685461520441 20-21 23.137017805913178 25.871556988190463 28.455055211047814 22.53636999484855 22-23 22.815644890472402 25.85945970121587 28.3196006224849 23.00529478582683 24-25 22.860674678299148 26.020369054911257 28.430352022996253 22.688604243793346 26-27 22.43459803690043 26.276344099203335 28.33751647064006 22.951541393256175 28-29 22.456848519335182 26.830637443501086 28.15188211153429 22.560631925629437 30-31 22.689413442810142 26.280334670538984 28.024036830679805 23.00621505597107 32-33 22.339512918501466 26.211789816509214 28.200661929056555 23.24803533593277 34-35 23.03027910582449 26.376013729351875 27.991756245262618 22.601950919561013 36-37 23.11686691154841 25.94466754264959 28.075836710350377 22.862628835451623 38-39 22.612483488776544 26.369490021312497 28.081200118521043 22.936826371389916 40-41 23.020908390787934 26.255616341053166 28.080304043657772 22.643171224501124 42-43 22.757853393971434 26.265103613442342 27.809214167500627 23.167828825085593 44-45 22.805211853115605 26.419002425728078 27.965953048703433 22.809832672452885 46-47 23.196210090179562 26.70491584422044 27.352059704605058 22.746814360994946 48-49 22.95753066980929 26.750057725224696 27.320048852918283 22.97236275204773 50-51 22.931672654243187 26.854590532210864 27.4825451412258 22.731191672320154 52-53 23.316354009472533 26.610873610730547 27.286274579538112 22.78649780025881 54-55 22.871604946254635 26.737280874755093 27.37260398278252 23.018510196207757 56-57 22.866512769777582 26.999985989779006 27.191629647351963 22.941871593091452 58-59 22.996502676880215 26.576597573428966 27.239283722358888 23.18761602733193 60-61 22.64846236133229 26.81920451820296 27.573352087324622 22.958981033140134 62-63 22.641824626049818 26.75637797734481 27.692905431029363 22.90889196557601 64-65 22.491925777777897 26.938426292528927 27.438412416748687 23.131235512944485 66-67 22.411231582892512 26.913960587127267 27.323237126851748 23.351570703128473 68-69 22.814610701387384 26.511568004259257 27.519962580602055 23.153858713751305 70-71 22.777042091295474 26.561863898749184 27.237668644712826 23.42342536524252 72-73 22.688582963140185 26.65754385738026 27.30482953871886 23.34904364076069 74-75 22.494756944219922 27.105426374133252 27.059044459819763 23.34077222182706 76-77 22.669232152529403 26.71128995588524 27.20846534730994 23.411012544275422 78-79 22.644138898187897 26.82310102133398 27.250858296393638 23.28190178408449 80-81 22.795765269796664 26.70897714574011 27.10766202719825 23.38759555726498 82-83 22.666113081007303 26.797717563811773 27.237263140396067 23.29890621478486 84-85 22.498205614003325 26.91724485771004 27.11741589017711 23.467133638109523 86-87 22.680546536741804 26.81357049947708 27.02354871272027 23.48233425106085 88-89 22.537092935366882 26.886825630335075 26.796632838508373 23.779448595789667 90-91 22.373702599041888 26.786532767408666 27.001736697882865 23.838027935666574 92-93 22.531951386688565 26.587789122464418 27.12054124716832 23.759718243678694 94-95 22.320895490217634 26.33307252768096 27.365962599176196 23.980069382925205 96-97 22.348501330499676 26.059364816908616 27.41015237329808 24.181981479293633 98-99 22.126874955964812 26.375861321525772 27.086567351574644 24.41069637093477 100-101 22.26899665584279 25.609368382141362 27.71516089782484 24.406474064191006 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 201.0 2 194.0 3 270.0 4 242.5 5 246.5 6 430.5 7 871.0 8 1443.5 9 1782.0 10 1724.0 11 1647.0 12 1812.0 13 2006.0 14 2224.0 15 2436.0 16 2704.0 17 3036.5 18 3324.5 19 3784.0 20 4414.0 21 5376.0 22 6771.0 23 8517.5 24 10686.5 25 13850.0 26 18375.5 27 24090.0 28 28737.5 29 33337.5 30 39062.5 31 46910.0 32 58590.0 33 63957.5 34 64611.5 35 69089.5 36 76644.5 37 84306.5 38 90201.0 39 95105.0 40 99951.0 41 106975.0 42 112029.5 43 115774.5 44 121632.0 45 129283.5 46 134121.0 47 140970.5 48 150424.5 49 148739.0 50 141590.0 51 137823.5 52 137373.0 53 139362.5 54 139148.0 55 130142.0 56 118928.0 57 109479.5 58 100897.5 59 90086.0 60 73536.5 61 61199.0 62 51557.5 63 37906.5 64 26321.5 65 19780.0 66 15017.0 67 12128.0 68 9573.0 69 7516.5 70 5882.0 71 3744.5 72 2655.5 73 2149.0 74 1519.5 75 1082.5 76 797.5 77 513.0 78 324.5 79 205.5 80 122.0 81 71.0 82 41.5 83 24.0 84 12.5 85 8.5 86 4.5 87 3.5 88 1.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3430079297850832 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013606080004828138 16-17 0.0019937622185292716 18-19 4.0414099024241995E-5 20-21 0.0 22-23 0.0 24-25 3.906696239010059E-4 26-27 0.0012797798024343297 28-29 0.0 30-31 0.0028289869316969396 32-33 0.0 34-35 0.010857921271179682 36-37 0.02903079446574717 38-39 0.022699252285282585 40-41 0.014279648321898838 42-43 0.010184352954108982 44-45 0.0030445287931595635 46-47 9.834097429232218E-4 48-49 7.274537824363559E-4 50-51 5.388546536565599E-5 52-53 8.082819804848399E-5 54-55 0.0 56-57 0.0 58-59 0.004472493625349447 60-61 0.009861040161915045 62-63 0.0041087667341312695 64-65 0.0019668194858464437 66-67 0.004014467169741371 68-69 0.0035564407141332956 70-71 2.6942732682827994E-5 72-73 0.0 74-75 0.0 76-77 0.21699676902749665 78-79 2.2901322780403797E-4 80-81 0.0033274274863292572 82-83 4.1761235658383395E-4 84-85 0.0 86-87 0.10907765326642915 88-89 0.4054881268765614 90-91 0.39383539499123826 92-93 0.0 94-95 0.0 96-97 1.3471366341413997E-5 98-99 0.0014953216638969537 100-101 7.543965151191839E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3711576.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.634499550901914 #Duplication Level Percentage of deduplicated Percentage of total 1 70.88039407638146 33.76352099799143 2 15.229838171409934 14.509314390726708 3 5.602932714123627 8.006786875639667 4 2.6532624436892887 5.055473147293694 5 1.5093584235424864 3.59487665741923 6 0.905945445023066 2.5892554736455726 7 0.6316255631678875 2.106101732354124 8 0.42313193820860595 1.612454249645608 9 0.32982088899965545 1.4139767690038942 >10 1.6478245453423306 14.158629121689323 >50 0.12015502229572755 3.9098169500337754 >100 0.059268780866238026 5.192272875237107 >500 0.003390519460409368 1.1075791552842436 >1k 0.002938450174873563 2.598438231553679 >5k 1.1301731441821392E-4 0.3815033724820558 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7689 0.20716267159826446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6484 0.17469667871545672 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4680 0.126091988955635 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4177 0.11253979441617254 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4013 0.10812118625618874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3888 0.10475334467083525 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3825 0.10305595251181708 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3471366341413998E-4 2 0.0 0.0 0.0 0.0 1.3471366341413998E-4 3 0.0 0.0 0.0 0.0 1.3471366341413998E-4 4 0.0 0.0 0.0 0.0 1.3471366341413998E-4 5 0.0 0.0 0.0 0.0 1.3471366341413998E-4 6 0.0 0.0 0.0 0.0 1.6165639609696798E-4 7 0.0 0.0 0.0 0.0 1.6165639609696798E-4 8 0.0 0.0 0.0 0.0 1.6165639609696798E-4 9 0.0 0.0 0.0 0.0 1.6165639609696798E-4 10-11 0.0 0.0 0.0 0.0 1.6165639609696798E-4 12-13 0.0 0.0 0.0 0.0 3.3678415853534996E-4 14-15 0.0 0.0 0.0 0.0 3.7719825755959196E-4 16-17 0.0 0.0 0.0 0.0 4.4455508926666195E-4 18-19 0.0 0.0 0.0 2.6942732682827997E-5 4.8496918829090394E-4 20-21 0.0 0.0 0.0 6.735683170706999E-5 5.119119209737319E-4 22-23 0.0 0.0 0.0 1.8859912877979598E-4 5.388546536565599E-4 24-25 0.0 0.0 0.0 2.8289869316969397E-4 5.657973863393879E-4 26-27 0.0 0.0 0.0 5.25383287315146E-4 6.33154218046458E-4 28-29 0.0 0.0 0.0 0.0022766609116989657 6.466255843878719E-4 30-31 0.0 0.0 0.0 0.008231004834603952 6.466255843878719E-4 32-33 0.0 0.0 0.0 0.01977596578919575 6.466255843878719E-4 34-35 0.0 0.0 0.0 0.03749081252815516 6.466255843878719E-4 36-37 0.0 0.0 0.0 0.06793610045975079 6.466255843878719E-4 38-39 0.0 0.0 0.0 0.11755114269517855 6.735683170706999E-4 40-41 0.0 0.0 0.0 0.17031848465449717 6.735683170706999E-4 42-43 0.0 0.0 0.0 0.22638631136746223 6.735683170706999E-4 44-45 0.0 0.0 0.0 0.2942415836291645 7.274537824363559E-4 46-47 0.0 0.0 0.0 0.3758645922917919 7.813392478020119E-4 48-49 0.0 0.0 0.0 0.4667424296309708 7.94810614143426E-4 50-51 0.0 0.0 0.0 0.5676833776271859 8.621674458504959E-4 52-53 0.0 0.0 0.0 0.665013999443902 0.0010238238419474639 54-55 0.0 0.0 0.0 0.7780387630483654 0.0010238238419474639 56-57 0.0 0.0 0.0 0.9120923295117762 0.001064237940971706 58-59 0.0 0.0 0.0 1.0629851039019544 0.0011046520399959478 60-61 0.0 0.0 0.0 1.232737791170112 0.0011315947726787759 62-63 0.0 0.0 0.0 1.4072189280241063 0.0011854802380444319 64-65 0.0 0.0 0.0 1.5906315807624578 0.0012124229707272598 66-67 0.0 0.0 0.0 1.8076552925226372 0.0012258943370686737 68-69 0.0 0.0 0.0 2.0498839307076024 0.0012393657034100879 70-71 0.0 0.0 0.0 2.3126698739295652 0.001266308436092916 72-73 0.0 0.0 0.0 2.5904090337904977 0.001387550733165642 74-75 0.0 0.0 0.0 2.8745740353962845 0.0014818502975555397 76-77 0.0 0.0 0.0 3.190720060696588 0.0014818502975555397 78-79 0.0 0.0 0.0 3.5521568196367257 0.0014818502975555397 80-81 0.0 2.6942732682827997E-5 0.0 3.946207756489427 0.0014818502975555397 82-83 0.0 2.6942732682827997E-5 0.0 4.355696879169388 0.0015626784956040238 84-85 0.0 2.6942732682827997E-5 0.0 4.78282002039026 0.0015626784956040238 86-87 0.0 2.6942732682827997E-5 0.0 5.228291162568138 0.0016300353273110938 88-89 0.0 2.6942732682827997E-5 0.0 5.715644782701473 0.0016973921590181637 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15360 0.0 25.2615 1 TAAGGTG 1840 0.0 24.755028 5 GGTATCA 11170 0.0 24.550596 1 GTAAGGT 1890 0.0 22.342833 4 GTACATG 11635 0.0 22.096329 1 TACATGG 11305 0.0 22.076246 2 TCAACGC 17575 0.0 21.624557 4 ATCAACG 17670 0.0 21.508297 3 CAACGCA 17765 0.0 21.500113 5 TATCAAC 18030 0.0 21.157793 2 AACGCAG 18275 0.0 20.926071 6 ACATGGG 11280 0.0 20.779156 3 GAGTACT 10370 0.0 19.882088 12-13 GGTAAGG 2035 0.0 19.118748 3 ACGCAGA 20405 0.0 18.625414 7 CGCAGAG 20505 0.0 18.55772 8 GTACTTT 11015 0.0 18.524006 14-15 AGAGTAC 15555 0.0 18.471199 10-11 GTGTAGC 2325 0.0 18.429445 1 AGGTAAG 1995 0.0 17.837269 2 >>END_MODULE