FastQCFastQC Report
Fri 27 May 2016
SRR939170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648721
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72280.19809681255431696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57460.15747984019605774No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39540.10836674001657019No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39330.10779119587384182No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39060.10705121054747678No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37840.10370757314686434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134800.028.8840751
GGTATCA93000.026.44731
ATCAACG155500.024.548743
TCAACGC155700.024.5476254
CAACGCA157400.024.2818325
TATCAAC159250.024.088972
TACATGG108600.023.6800522
AACGCAG161950.023.5285536
GTACATG113500.023.1346951
ACATGGG109250.022.4560243
ACGCAGA179800.021.1137317
CGCAGAG180450.021.0111568
GAGTACT91800.020.10995712-13
AGAGTAC140100.019.44381710-11
GAGTACA83500.018.765581
GTACTTT96850.018.6711414-15
TAAGGTG19050.018.6457125
GTAAGGT17300.018.3422784
CATGGGG72550.018.2134134
GCAGAGT204300.018.0717649
Produced by FastQC (version 0.11.5)