##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3648721 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.154955119889955 33.0 31.0 34.0 30.0 34.0 2 32.258159503014895 34.0 31.0 34.0 30.0 34.0 3 32.32896541007109 34.0 31.0 34.0 30.0 34.0 4 35.754814906374044 37.0 35.0 37.0 35.0 37.0 5 35.79019004193524 37.0 35.0 37.0 35.0 37.0 6 35.87096218099438 37.0 37.0 37.0 35.0 37.0 7 35.79906685109659 37.0 36.0 37.0 35.0 37.0 8 35.7905424942055 37.0 36.0 37.0 35.0 37.0 9 37.48192805095265 39.0 38.0 39.0 35.0 39.0 10-11 37.39395475839342 39.0 38.0 39.0 35.0 39.0 12-13 37.302671127773266 39.0 37.0 39.0 34.0 39.0 14-15 38.63127970047587 40.0 38.0 41.0 34.0 41.0 16-17 38.5403619514893 40.0 38.0 41.0 34.0 41.0 18-19 38.42493561442489 40.0 38.0 41.0 34.0 41.0 20-21 38.39224744780431 40.0 38.0 41.0 34.0 41.0 22-23 38.43524443222708 40.0 38.0 41.0 34.0 41.0 24-25 38.391228734671685 40.0 38.0 41.0 34.0 41.0 26-27 38.28228946526742 40.0 38.0 41.0 34.0 41.0 28-29 38.14644926263203 40.0 38.0 41.0 33.5 41.0 30-31 37.95311918888838 40.0 38.0 41.0 33.0 41.0 32-33 37.76749989927977 40.0 38.0 41.0 33.0 41.0 34-35 37.47126088840446 40.0 37.5 41.0 32.0 41.0 36-37 37.2203563933773 40.0 37.0 41.0 31.0 41.0 38-39 37.03442576179434 40.0 37.0 41.0 31.0 41.0 40-41 36.848116230317416 40.0 37.0 41.0 30.5 41.0 42-43 36.64617601619855 40.0 36.0 41.0 30.0 41.0 44-45 36.40203896104964 40.0 36.0 41.0 30.0 41.0 46-47 36.32879096538212 40.0 36.0 41.0 30.0 41.0 48-49 36.22954372230708 40.0 36.0 41.0 29.0 41.0 50-51 35.099877737979966 38.5 34.5 40.0 27.5 40.5 52-53 35.23301452755637 38.5 35.0 39.5 27.5 40.5 54-55 35.76122605702108 39.0 35.0 41.0 28.0 41.0 56-57 35.65773280555022 39.0 35.0 41.0 28.0 41.0 58-59 35.43907974876676 39.0 35.0 41.0 28.0 41.0 60-61 35.13921206910586 38.5 35.0 40.5 27.0 41.0 62-63 34.7554441679701 38.0 34.5 40.0 26.0 41.0 64-65 34.38133362348067 37.0 34.0 40.0 26.0 41.0 66-67 33.99585676734395 37.0 34.0 39.5 25.5 41.0 68-69 33.5866554335067 36.0 34.0 39.0 24.5 41.0 70-71 33.1546123970564 35.5 33.0 39.0 23.5 40.5 72-73 32.721291241506265 35.0 33.0 38.0 22.0 40.0 74-75 32.286578639473944 35.0 32.5 37.0 21.5 39.5 76-77 31.859548044369518 35.0 32.0 37.0 20.0 39.0 78-79 31.43572144869394 35.0 32.0 36.0 18.5 39.0 80-81 31.008292083719198 35.0 31.0 36.0 17.0 37.5 82-83 30.624543778491148 35.0 31.0 35.0 11.5 37.0 84-85 30.26060529155285 34.5 31.0 35.0 7.0 37.0 86-87 29.874032297892878 34.0 30.5 35.0 3.5 36.0 88-89 29.456014861097906 34.0 30.0 35.0 2.0 36.0 90-91 29.054847986458817 34.0 29.5 35.0 2.0 35.5 92-93 28.655474754030248 34.0 29.0 35.0 2.0 35.0 94-95 28.180399926439975 34.0 29.0 35.0 2.0 35.0 96-97 27.598158779473685 34.0 28.0 35.0 2.0 35.0 98-99 26.897459822222636 34.0 26.5 35.0 2.0 35.0 100-101 23.975950339858816 30.0 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21932583793797988 1101 2 0.32536413212361026 1101 3 0.33776780873819945 1101 4 0.3219473167344944 1101 5 0.2871637124129762 1101 6 0.2507710011845603 1101 7 0.2082899487742651 1101 8 0.30750412200001875 1101 9 0.38180693408011734 1101 10-11 0.34346779242356007 1101 12-13 0.40124737892479345 1101 14-15 0.579667230593472 1101 16-17 0.5398732704515012 1101 18-19 0.6500165324822049 1101 20-21 0.616403749592429 1101 22-23 0.6045462967309518 1101 24-25 0.650140020863951 1101 26-27 0.5972644285313464 1101 28-29 0.6169124502819585 1101 30-31 0.6307469205638796 1101 32-33 0.5642644010068096 1101 34-35 0.6219310906867861 1101 36-37 0.5800940046339562 1101 38-39 0.5523631921466929 1101 40-41 0.5085276981981437 1101 42-43 0.44143365623302344 1101 44-45 0.5664312232129163 1101 46-47 0.7755326057099197 1101 48-49 0.7228510063464881 1101 50-51 0.772921826308071 1101 52-53 0.7229360943724075 1101 54-55 0.767867941090735 1101 56-57 0.8287227748523094 1101 58-59 0.7061927702682809 1101 60-61 0.6093693010615837 1101 62-63 0.5716762192113336 1101 64-65 0.5399338834582963 1101 66-67 0.5396265314579907 1101 68-69 0.5606625332006203 1101 70-71 0.39802484289594986 1101 72-73 0.4250481498077505 1101 74-75 0.4045129934974341 1101 76-77 0.29083160785473083 1101 78-79 0.19059297594774094 1101 80-81 0.0774996294617587 1101 82-83 0.009271803268969592 1101 84-85 -0.04280825442702252 1101 86-87 -0.056075137560167576 1101 88-89 -0.06525470343336792 1101 90-91 -0.03451429132577388 1101 92-93 -0.10703073854229928 1101 94-95 -0.24879102489390093 1101 96-97 -0.28323214059929924 1101 98-99 -0.5071215416021886 1101 100-101 -0.8001348956167327 1102 1 0.27119226681936226 1102 2 0.3076772606086635 1102 3 0.26536305466728294 1102 4 0.1702671458442495 1102 5 0.21253308791708747 1102 6 0.2140102220083122 1102 7 0.2838980910643514 1102 8 0.2860554098774557 1102 9 0.3410516676741082 1102 10-11 0.3342792558019738 1102 12-13 0.3496980629661621 1102 14-15 0.39881156536181095 1102 16-17 0.4165793540551519 1102 18-19 0.41206079382867955 1102 20-21 0.34626594842559655 1102 22-23 0.4700790112678206 1102 24-25 0.4878610627911897 1102 26-27 0.5091926580031156 1102 28-29 0.43823612615084073 1102 30-31 0.44694046205245996 1102 32-33 0.45520695045602366 1102 34-35 0.46369797294267556 1102 36-37 0.37475517166176786 1102 38-39 0.3516373949827951 1102 40-41 0.30004557319686853 1102 42-43 0.21198157872380818 1102 44-45 0.1875798664158097 1102 46-47 0.3536803145074998 1102 48-49 0.3155740511450844 1102 50-51 0.3523909980680031 1102 52-53 0.30468892135547065 1102 54-55 0.29344344250701937 1102 56-57 0.4252227848013419 1102 58-59 0.3029944168636902 1102 60-61 0.1312819497418687 1102 62-63 0.19078113972256006 1102 64-65 0.1866717456860698 1102 66-67 0.0713341556546041 1102 68-69 0.11169545405434178 1102 70-71 0.029617812003493782 1102 72-73 0.11087169975602507 1102 74-75 0.0478049723443732 1102 76-77 -0.11528894791644717 1102 78-79 -0.04365026529103133 1102 80-81 -0.05509316739684067 1102 82-83 -0.23982368877310734 1102 84-85 -0.3007126118429859 1102 86-87 -0.2561893203213259 1102 88-89 -0.2531209208726395 1102 90-91 -0.23012522939513858 1102 92-93 -0.3601109697699272 1102 94-95 -0.3750223686949603 1102 96-97 -0.3612295084263728 1102 98-99 -0.46164726709283954 1102 100-101 -0.6485030629663306 1103 1 0.1253684162651112 1103 2 0.21029171633677635 1103 3 0.23486939091134218 1103 4 0.1981042038113614 1103 5 0.1826662106281134 1103 6 0.24425537664665598 1103 7 0.22468301557970705 1103 8 0.2748839207653333 1103 9 0.2709909020750132 1103 10-11 0.3175369385883542 1103 12-13 0.3186258081525537 1103 14-15 0.343994727116538 1103 16-17 0.3750068013555108 1103 18-19 0.3186242209072603 1103 20-21 0.35177366466323434 1103 22-23 0.3021357816200876 1103 24-25 0.3733899751630787 1103 26-27 0.3498055657675394 1103 28-29 0.2945998253488682 1103 30-31 0.2877163185449447 1103 32-33 0.32076553623435444 1103 34-35 0.26492356398625816 1103 36-37 0.32552555484924284 1103 38-39 0.24122619214451646 1103 40-41 0.2770392311929868 1103 42-43 0.27546724007263634 1103 44-45 0.14022671989555846 1103 46-47 0.18622292806134766 1103 48-49 0.1650399977654402 1103 50-51 -0.008535905495591578 1103 52-53 0.11753201827136905 1103 54-55 0.0034444280760652646 1103 56-57 0.1628045902318931 1103 58-59 0.13624706350056925 1103 60-61 0.09198019341057773 1103 62-63 -0.018584542723942832 1103 64-65 -0.0498481854048336 1103 66-67 -0.026545305411424636 1103 68-69 -0.041141247000908265 1103 70-71 0.041730120795961057 1103 72-73 0.16312369548410288 1103 74-75 -0.005248524363018703 1103 76-77 -0.006479860910786783 1103 78-79 -0.08436982324411346 1103 80-81 -0.06543632560311963 1103 82-83 -0.19144603289344886 1103 84-85 -0.2046841695609487 1103 86-87 -0.27031904267146345 1103 88-89 -0.3700654323508772 1103 90-91 -0.33242498239243545 1103 92-93 -0.43388238805103896 1103 94-95 -0.4507910657815408 1103 96-97 -0.4139293423958925 1103 98-99 -0.4295586257416133 1103 100-101 -0.6646291779959235 1104 1 0.09298656570645392 1104 2 0.1989526266697652 1104 3 0.18134498642557872 1104 4 0.26608785584633665 1104 5 0.27062317014634374 1104 6 0.1876537846509052 1104 7 0.19690662774269896 1104 8 0.14535256073326508 1104 9 0.27680829891290415 1104 10-11 0.265925139715705 1104 12-13 0.2132888879239374 1104 14-15 0.3083215439363869 1104 16-17 0.35542121667150894 1104 18-19 0.3292528859336983 1104 20-21 0.3628962062702854 1104 22-23 0.4113124721155259 1104 24-25 0.3902602281030809 1104 26-27 0.4332790475352368 1104 28-29 0.3302041711235404 1104 30-31 0.46474252001210203 1104 32-33 0.40993033072147966 1104 34-35 0.4180295444726241 1104 36-37 0.4331196917681126 1104 38-39 0.37803057037312016 1104 40-41 0.40344576879587635 1104 42-43 0.4617699533406707 1104 44-45 0.478745333275576 1104 46-47 0.5619449787319013 1104 48-49 0.5508014256935283 1104 50-51 0.427111862970996 1104 52-53 0.5349840957051057 1104 54-55 0.45411274457886464 1104 56-57 0.5466894567735565 1104 58-59 0.5974206465596055 1104 60-61 0.6098873892799972 1104 62-63 0.5982919013420585 1104 64-65 0.5953771136279684 1104 66-67 0.5088778297804311 1104 68-69 0.41970454562012094 1104 70-71 0.4301079355320212 1104 72-73 0.435662009083579 1104 74-75 0.3572546242061918 1104 76-77 0.3117963147600058 1104 78-79 0.2971064408551847 1104 80-81 0.13224279370779257 1104 82-83 0.18058197926603725 1104 84-85 0.1335860920296703 1104 86-87 0.09008152600749142 1104 88-89 0.12549929021741946 1104 90-91 0.1833705871925666 1104 92-93 0.10409225434075609 1104 94-95 -0.011352151416691214 1104 96-97 0.05028312234790633 1104 98-99 0.13400077415452216 1104 100-101 -0.01015111394688617 1105 1 0.17003869527071203 1105 2 0.2221142706206365 1105 3 0.2502331466160257 1105 4 0.23840606431245703 1105 5 0.22002342316530843 1105 6 0.2653134725318651 1105 7 0.23600222788429193 1105 8 0.292106433906298 1105 9 0.33563412842012497 1105 10-11 0.30611477629353345 1105 12-13 0.31910614869256904 1105 14-15 0.3501386619616227 1105 16-17 0.347383046656212 1105 18-19 0.3888829054809122 1105 20-21 0.38855352252546993 1105 22-23 0.36678619591496187 1105 24-25 0.43656189775136056 1105 26-27 0.41828613706393014 1105 28-29 0.39358178906707053 1105 30-31 0.538268492208779 1105 32-33 0.45909262115251437 1105 34-35 0.4351691340296284 1105 36-37 0.44772900183750863 1105 38-39 0.452527476311154 1105 40-41 0.46259865865748395 1105 42-43 0.43446232388598816 1105 44-45 0.4171641877005072 1105 46-47 0.37971029312961946 1105 48-49 0.5155203012063581 1105 50-51 0.4792793602999481 1105 52-53 0.489985049425151 1105 54-55 0.4335368864420701 1105 56-57 0.4693583124119769 1105 58-59 0.5302924638897721 1105 60-61 0.5460429339170574 1105 62-63 0.4868476923463092 1105 64-65 0.4964851996817359 1105 66-67 0.3256999411278301 1105 68-69 0.4543767185143679 1105 70-71 0.4336713068577538 1105 72-73 0.4910886041915745 1105 74-75 0.46102403861129915 1105 76-77 0.44888709830079776 1105 78-79 0.37344085339330846 1105 80-81 0.4834757712433273 1105 82-83 0.4811659957092118 1105 84-85 0.3976464512720135 1105 86-87 0.17890337883473606 1105 88-89 0.36378862388801636 1105 90-91 0.3962713720834863 1105 92-93 0.24822065579990493 1105 94-95 0.20117415678427975 1105 96-97 0.2956032423889887 1105 98-99 0.3854274889943916 1105 100-101 0.12771118505953538 1106 1 0.09691833330333566 1106 2 0.14347737999678856 1106 3 0.22743137325380047 1106 4 0.2536726114233616 1106 5 0.3006934275509465 1106 6 0.19975491119584632 1106 7 0.22136591043334874 1106 8 0.18775353308691933 1106 9 0.21163051569394753 1106 10-11 0.2434055790395533 1106 12-13 0.2378413471536902 1106 14-15 0.24142630357223993 1106 16-17 0.2331167296829122 1106 18-19 0.22972516569272727 1106 20-21 0.30016461602647126 1106 22-23 0.33052254506410605 1106 24-25 0.3873861377544827 1106 26-27 0.3155150197675738 1106 28-29 0.3550424246810806 1106 30-31 0.34979439137377 1106 32-33 0.3349979520947315 1106 34-35 0.3651349173442213 1106 36-37 0.3636830524764676 1106 38-39 0.32222942640169805 1106 40-41 0.46326757748838787 1106 42-43 0.5194079810156964 1106 44-45 0.3255121470747042 1106 46-47 0.377912093413606 1106 48-49 0.5193712849360637 1106 50-51 0.3818283296742777 1106 52-53 0.4281433406751418 1106 54-55 0.4398892488262973 1106 56-57 0.45597177242231623 1106 58-59 0.4977844850624322 1106 60-61 0.5027818840553593 1106 62-63 0.42090944738437486 1106 64-65 0.42153298580139875 1106 66-67 0.35415858366334163 1106 68-69 0.3485786403804809 1106 70-71 0.40136085557093537 1106 72-73 0.40882509229172825 1106 74-75 0.379806567259088 1106 76-77 0.3577861721054951 1106 78-79 0.35461538019999495 1106 80-81 0.3977244153854578 1106 82-83 0.448161440721524 1106 84-85 0.4968166532286169 1106 86-87 0.49608792748278674 1106 88-89 0.40183827281961015 1106 90-91 0.3218424390101049 1106 92-93 0.2727655695417255 1106 94-95 0.4589084737749438 1106 96-97 0.5608788213153311 1106 98-99 0.6029026189352926 1106 100-101 0.39442508059443426 1107 1 0.09544770545837622 1107 2 0.13779153245891962 1107 3 0.16071948412480452 1107 4 0.14904479546524385 1107 5 0.125116292105254 1107 6 0.13917805545536766 1107 7 0.09663313250478467 1107 8 0.1696602938726599 1107 9 0.12737145479901812 1107 10-11 0.13921418608688185 1107 12-13 0.17963742755132728 1107 14-15 0.12081024766434467 1107 16-17 0.12139732995101582 1107 18-19 0.1428599152322505 1107 20-21 0.14134198849643553 1107 22-23 0.12943579708264963 1107 24-25 0.17723996588288315 1107 26-27 0.20460162686048022 1107 28-29 0.16480144310225597 1107 30-31 0.2728208846370137 1107 32-33 0.2550033804477252 1107 34-35 0.21965062330981056 1107 36-37 0.2080431387499857 1107 38-39 0.20032042797425476 1107 40-41 0.21523038291093854 1107 42-43 0.21722619749095173 1107 44-45 0.2528033367749174 1107 46-47 0.4861330646744122 1107 48-49 0.39521126040488497 1107 50-51 0.35909438734327637 1107 52-53 0.3645843301271512 1107 54-55 0.3240313307861342 1107 56-57 0.36502725972059125 1107 58-59 0.3778871265155672 1107 60-61 0.18562128561051594 1107 62-63 0.3262117051234057 1107 64-65 0.4642411952529457 1107 66-67 0.38071396218736453 1107 68-69 0.3917803327006055 1107 70-71 0.38714354326867806 1107 72-73 0.4028649264576174 1107 74-75 0.3689179955996522 1107 76-77 0.4567410276843695 1107 78-79 0.5125433654315579 1107 80-81 0.4717255880754543 1107 82-83 0.36097380410624424 1107 84-85 0.39421290849380597 1107 86-87 0.2458984531006756 1107 88-89 0.39153836667625086 1107 90-91 0.3850549465984052 1107 92-93 0.287503009223812 1107 94-95 0.522334178891068 1107 96-97 0.4573840393714441 1107 98-99 0.3589697150357871 1107 100-101 0.39901995349431374 1108 1 -0.015758814683643152 1108 2 0.04306292327810013 1108 3 0.0507863164942961 1108 4 0.11527045276884706 1108 5 0.09947978218292519 1108 6 0.12775339403606978 1108 7 0.029311622498688905 1108 8 0.20732563631150214 1108 9 0.15411193770142262 1108 10-11 0.12874383764521014 1108 12-13 0.21574140013059662 1108 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-1.4468076320559575 2316 50-51 -1.5213399856294956 2316 52-53 -1.521968848951552 2316 54-55 -1.440578490869079 2316 56-57 -1.3938864087785916 2316 58-59 -1.2055623768687127 2316 60-61 -1.1466391125342028 2316 62-63 -1.2019306322440286 2316 64-65 -1.465055801408674 2316 66-67 -1.4637573435600046 2316 68-69 -1.3691804991389702 2316 70-71 -1.3176974922940659 2316 72-73 -1.570596212935044 2316 74-75 -1.2886079633808691 2316 76-77 -1.3551039067513138 2316 78-79 -1.1025797248138183 2316 80-81 -1.214007039376046 2316 82-83 -1.1835024224904664 2316 84-85 -1.0486988480753787 2316 86-87 -1.159097949278376 2316 88-89 -1.2572126677522917 2316 90-91 -1.3835142684481134 2316 92-93 -1.4450568406062665 2316 94-95 -1.3534774461954235 2316 96-97 -1.3951860542267127 2316 98-99 -1.8065672966524318 2316 100-101 -1.7532536589311896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10991.0 3 2830.0 4 3026.0 5 3406.0 6 3682.0 7 4690.0 8 5583.0 9 7119.0 10 8374.0 11 10277.0 12 13388.0 13 20975.0 14 17616.0 15 17184.0 16 19858.0 17 25858.0 18 22802.0 19 18417.0 20 18203.0 21 19085.0 22 20931.0 23 23585.0 24 26640.0 25 29983.0 26 34548.0 27 40245.0 28 47568.0 29 56941.0 30 68467.0 31 85171.0 32 107120.0 33 140872.0 34 197066.0 35 300735.0 36 497425.0 37 807805.0 38 771448.0 39 138755.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.848494428307575 18.17705328304902 13.69705544013495 24.27739684850846 2 15.628434162988238 19.601464913606918 39.79596904550951 24.97413187789533 3 19.860635686758197 23.027270569544275 29.05099735073352 28.061096392964007 4 13.27751695128283 15.555361018141742 35.96822368558266 35.19889834499278 5 14.35086809343273 36.567281529019006 34.030387729647174 15.051462647901085 6 33.82398759874978 34.638944138423575 16.92492548169488 14.612142781131757 7 29.601375415005364 31.75306714474114 20.21333963841595 18.432217801837545 8 26.627346988706584 34.77737104350655 19.61149166639695 18.983790301389913 9 26.298310354720734 16.859314717484573 20.0661628249513 36.776212102843395 10-11 25.51997442623949 25.91150749281835 26.906097479528167 21.66242060141399 12-13 26.198653291930203 23.04750233077759 27.414607413120763 23.33923696417144 14-15 22.81958908505462 24.682186828889662 25.45641674049166 27.041807345564056 16-17 22.83492480611661 27.8107726083921 26.354536955680718 22.99976562981057 18-19 22.82564374687722 26.695634081776326 28.105474270903436 22.373247900443012 20-21 23.192231078429277 25.804231559001483 28.350633384298124 22.652903978271112 22-23 22.83481998651128 25.843638129811286 28.240580906454486 23.08096097722294 24-25 22.882159377292226 25.969853145234783 28.33680609713889 22.8111813803341 26-27 22.47446018595786 26.192423870989252 28.263964844781558 23.069151098271334 28-29 22.490535921249208 26.78300335544922 28.050148060345688 22.676312662955887 30-31 22.701206457611722 26.30181561864643 27.882943202989352 23.1140347207525 32-33 22.335889205986017 26.226482147136025 28.116181083780894 23.321447563097063 34-35 23.0830958450015 26.327956925671263 27.890457355364035 22.698489873963204 36-37 23.11698212918637 25.932648886365023 27.968218031117665 22.982150953330944 38-39 22.64587527229477 26.344511164171617 27.96436115153398 23.045252411999634 40-41 23.031839092789443 26.22169700446409 28.020084346654922 22.726379556091544 42-43 22.7684620934491 26.21836139149685 27.759165998855657 23.254010516198402 44-45 22.79741370463242 26.397864489352408 27.86496735722981 22.939754448785365 46-47 23.223176948238446 26.638247121932217 27.28528375969997 22.853292170129365 48-49 22.927236496711352 26.725948865315296 27.29354262566873 23.053272012304614 50-51 22.91215108383631 26.815942754509432 27.442630114220425 22.829276047433837 52-53 23.29974110158973 26.552350404685466 27.301246497901015 22.84666199582379 54-55 22.84092990965104 26.732243929787053 27.376656937061533 23.050169223500372 56-57 22.872546400469307 26.937610337961214 27.191459945082336 22.998383316487143 58-59 22.932554759963285 26.546909238316097 27.26761008653477 23.252925915185845 60-61 22.66792836920543 26.730753669093737 27.620730125019747 22.980587836681085 62-63 22.645235324968375 26.734417075438856 27.76265541055712 22.85769218903565 64-65 22.52137027075691 26.875258565500985 27.491848169373473 23.111522994368627 66-67 22.43644095235445 26.864914479831647 27.373356549841045 23.325288017972863 68-69 22.853514005048147 26.472956360681444 27.56849015401715 23.105039480253257 70-71 22.80663438068485 26.531831859721606 27.277350877594277 23.384182881999266 72-73 22.699340099096045 26.605715926417677 27.425573602090104 23.26937037239617 74-75 22.569870318682447 27.034645890173337 27.188422178947047 23.20706161219717 76-77 22.770532359196448 26.690551753776987 27.222814528303967 23.3161013587226 78-79 22.802199920790073 26.73996063740306 27.33470986747485 23.12312957433202 80-81 22.94197694546483 26.669013408517213 27.19101197871241 23.197997667305547 82-83 22.816972683483026 26.73822019023671 27.329257822846557 23.115549303433706 84-85 22.675568093310723 26.928808098660173 27.19634948218174 23.199274325847362 86-87 22.87625692603138 26.83284596825743 27.14001612292095 23.150880982790234 88-89 22.78843441311429 26.886912265776864 26.876416027892997 23.448237293215847 90-91 22.684708595008896 26.746443269767024 27.092954433733716 23.475893701490367 92-93 22.859354425546975 26.575450689238362 27.254844660447 23.310350224767664 94-95 22.715751688799372 26.31362352121989 27.49372680488431 23.476897985096436 96-97 22.825245928742664 26.014204623735914 27.54171833236891 23.618831115152517 98-99 22.646207048579537 26.299991733487644 27.245005648783444 23.80879556914938 100-101 22.964713621873102 25.57422183629774 27.744716464882767 23.716348076946385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2825.0 1 1526.5 2 307.5 3 407.5 4 379.0 5 384.0 6 621.0 7 1124.0 8 1781.5 9 2154.0 10 2169.0 11 2229.0 12 2428.5 13 2798.5 14 3239.5 15 3582.5 16 3898.5 17 4185.0 18 4452.0 19 4794.5 20 5370.5 21 6053.0 22 7094.5 23 8802.5 24 10857.0 25 13746.0 26 18235.5 27 23849.5 28 28133.5 29 32288.5 30 37730.0 31 45280.5 32 56591.0 33 62036.0 34 63082.0 35 67882.0 36 75077.0 37 82450.5 38 88202.5 39 92956.0 40 98266.5 41 105519.5 42 109811.0 43 113522.5 44 119199.0 45 126665.5 46 131972.5 47 137661.5 48 146859.0 49 145963.0 50 138149.5 51 133582.5 52 133227.0 53 134974.0 54 134316.5 55 126116.0 56 115600.0 57 106535.0 58 98445.0 59 87832.0 60 71922.5 61 59895.5 62 50750.5 63 38029.0 64 26866.0 65 20305.0 66 15304.0 67 12369.0 68 9856.0 69 7822.0 70 6280.5 71 4203.0 72 2922.0 73 2351.0 74 1735.5 75 1214.0 76 933.5 77 638.0 78 435.0 79 299.5 80 197.0 81 144.5 82 102.5 83 79.5 84 60.5 85 37.5 86 30.5 87 27.0 88 19.0 89 17.0 90 17.0 91 9.5 92 5.0 93 8.5 94 8.5 95 5.0 96 3.5 97 3.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3888485855728624 2 0.11713693647719296 3 0.11828802476264971 4 0.1301551968484299 5 0.13199145673237278 6 0.07227190020831957 7 0.08180948885924684 8 0.07890436128166554 9 0.07857547891439219 10-11 0.07664329500666124 12-13 0.08063099370985066 14-15 0.07430000813983859 16-17 0.07617737831969065 18-19 0.08579718756243626 20-21 0.08405685170228143 22-23 0.09462219775093793 24-25 0.10769527185005376 26-27 0.10881895327157105 28-29 0.10289907066065068 30-31 0.09262149668335837 32-33 0.09114152603062826 34-35 0.3398999265770115 36-37 0.5308024373472239 38-39 0.5123302110520371 40-41 0.4833063421401636 42-43 0.5125631693955224 44-45 0.5221144614784194 46-47 0.5191408167409894 48-49 0.525896608702063 50-51 0.5251155130797888 52-53 0.5237999836106954 54-55 0.5181541696391695 56-57 0.4886917909042648 58-59 0.4786334718384881 60-61 0.42576563129929634 62-63 0.5025596640576246 64-65 0.5295417216060093 66-67 0.5397507784234531 68-69 0.5553041737090887 70-71 0.5510972200943838 72-73 0.5375993396042066 74-75 0.5412307490761831 76-77 0.5163727234831055 78-79 0.4895688105503271 80-81 0.4975853182526151 82-83 0.5115354119977932 84-85 0.5054236813392967 86-87 0.5066295833526324 88-89 0.516591978394621 90-91 0.5356671556964756 92-93 0.5413266730999712 94-95 0.5368867611417809 96-97 0.5377500773558734 98-99 0.5377500773558734 100-101 0.5142898018237075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3648721.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.35011179639493 #Duplication Level Percentage of deduplicated Percentage of total 1 72.22409221075158 35.64267024993726 2 14.732324989586207 14.540837705138044 3 5.286393543656147 7.826523371375135 4 2.54238213142475 5.01867369659873 5 1.4017317486313594 3.458780925175687 6 0.8573534439653436 2.538629298522835 7 0.5871020064508756 2.0281484757966943 8 0.37718235563421887 1.4891193134541034 9 0.2899628729242527 1.2878730176054156 >10 1.533542880530439 13.697835667091521 >50 0.10793309913142639 3.6544496361774557 >100 0.0538168313533324 4.878934563441304 >500 0.0033972526583130277 1.140822190480835 >1k 0.002673247969791891 2.4401193750481056 >5k 1.1138533207466211E-4 0.35658251415685743 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7228 0.19809681255431696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5746 0.15747984019605774 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3954 0.10836674001657019 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3933 0.10779119587384182 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3906 0.10705121054747678 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3784 0.10370757314686434 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.222059181833854E-5 2 0.0 0.0 0.0 0.0 8.222059181833854E-5 3 0.0 0.0 0.0 0.0 8.222059181833854E-5 4 0.0 0.0 0.0 0.0 8.222059181833854E-5 5 0.0 0.0 0.0 0.0 8.222059181833854E-5 6 0.0 0.0 0.0 0.0 1.0962745575778471E-4 7 0.0 0.0 0.0 0.0 1.0962745575778471E-4 8 0.0 0.0 0.0 0.0 1.0962745575778471E-4 9 0.0 0.0 0.0 0.0 1.6444118363667708E-4 10-11 0.0 0.0 0.0 0.0 1.6444118363667708E-4 12-13 0.0 0.0 0.0 0.0 4.248063910614158E-4 14-15 0.0 0.0 0.0 0.0 5.207304148494773E-4 16-17 0.0 0.0 0.0 1.370343196972309E-5 6.16654438637539E-4 18-19 0.0 0.0 0.0 2.740686393944618E-5 6.851715984861545E-4 20-21 0.0 0.0 0.0 8.222059181833854E-5 7.262818943953237E-4 22-23 0.0 0.0 0.0 2.740686393944618E-4 8.222059181833853E-4 24-25 0.0 0.0 0.0 3.425857992430773E-4 8.222059181833853E-4 26-27 0.0 0.0 0.0 6.303578706072621E-4 8.496127821228316E-4 28-29 0.0 0.0 0.0 0.002644762370156556 8.496127821228316E-4 30-31 0.0 0.0 0.0 0.0077013287669843765 8.633162140925547E-4 32-33 0.0 0.0 0.0 0.01956850085276457 9.044265100017239E-4 34-35 0.0 0.0 0.0 0.03841071981113382 9.318333739411701E-4 36-37 0.0 0.0 0.0 0.07043564032437667 9.318333739411701E-4 38-39 0.0 0.0 0.0 0.11965836795962201 9.318333739411701E-4 40-41 0.0 0.0 0.0 0.17243028447502562 9.318333739411701E-4 42-43 0.0 0.0 0.0 0.22764141188103998 9.318333739411701E-4 44-45 0.0 0.0 0.0 0.2979948316135983 9.318333739411701E-4 46-47 0.0 0.0 0.0 0.3831890681693667 9.318333739411701E-4 48-49 0.0 0.0 0.0 0.4757420476928765 9.729436698503392E-4 50-51 0.0 0.0 0.0 0.5783807531461025 0.0010140539657595086 52-53 0.0 0.0 0.0 0.6781006275897774 0.0010688676936384009 54-55 0.0 0.0 0.0 0.7922776227615101 0.0010688676936384009 56-57 0.0 0.0 0.0 0.9307233959516226 0.001082571125608124 58-59 0.0 0.0 0.0 1.0832151869107012 0.0011236814215172934 60-61 0.0 0.0 0.0 1.2520962825055684 0.0011236814215172934 62-63 0.0 0.0 0.0 1.4221147629539228 0.0011510882854567394 64-65 0.0 0.0 0.0 1.6064807366745772 0.0011510882854567394 66-67 0.0 0.0 0.0 1.824433822153023 0.0011921985813659088 68-69 0.0 0.0 0.0 2.069341558315914 0.0012470123092448012 70-71 0.0 0.0 0.0 2.330090461835805 0.0012744191731842473 72-73 0.0 0.0 0.0 2.6056664787469366 0.0014662672207603705 74-75 0.0 0.0 0.0 2.8916434005230873 0.0016581152683364938 76-77 0.0 0.0 0.0 3.20623308825202 0.0018088530200034477 78-79 0.0 0.0 0.0 3.5729095208978707 0.0018636667478823402 80-81 0.0 0.0 0.0 3.9673079964184708 0.0018636667478823402 82-83 0.0 0.0 0.0 4.3723814454434855 0.0019184804757612325 84-85 0.0 0.0 0.0 4.789623542057614 0.0019184804757612325 86-87 0.0 0.0 0.0 5.235135818825281 0.0020144044995492943 88-89 0.0 0.0 0.0 5.717044410904533 0.002110328523337356 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13480 0.0 28.884075 1 GGTATCA 9300 0.0 26.4473 1 ATCAACG 15550 0.0 24.54874 3 TCAACGC 15570 0.0 24.547625 4 CAACGCA 15740 0.0 24.281832 5 TATCAAC 15925 0.0 24.08897 2 TACATGG 10860 0.0 23.680052 2 AACGCAG 16195 0.0 23.528553 6 GTACATG 11350 0.0 23.134695 1 ACATGGG 10925 0.0 22.456024 3 ACGCAGA 17980 0.0 21.113731 7 CGCAGAG 18045 0.0 21.011156 8 GAGTACT 9180 0.0 20.109957 12-13 AGAGTAC 14010 0.0 19.443817 10-11 GAGTACA 8350 0.0 18.76558 1 GTACTTT 9685 0.0 18.67114 14-15 TAAGGTG 1905 0.0 18.645712 5 GTAAGGT 1730 0.0 18.342278 4 CATGGGG 7255 0.0 18.213413 4 GCAGAGT 20430 0.0 18.071764 9 >>END_MODULE