FastQCFastQC Report
Fri 27 May 2016
SRR939170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3648721
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74760.20489371481129964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62710.17186844376426696No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45110.12363236323084172No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40890.11206666664839542No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA39960.10951782830202694No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38840.10644825954080897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37350.10236463681383148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150000.026.5916581
GGTATCA107450.024.7035851
ATCAACG170950.023.0658993
TACATGG111200.022.957042
GTACATG113950.022.8762171
TCAACGC172200.022.8709074
CAACGCA174400.022.6096085
TATCAAC176850.022.4305382
AACGCAG180600.021.8859636
ACATGGG112350.021.66623
GAGTACT103800.020.70975712-13
AGAGTAC152200.019.87635210-11
GTATAGG10100.019.8170551
ACGCAGA199850.019.6591437
GTACTTT109300.019.55909514-15
TAAGGTG18200.019.553675
CATGGGG74350.019.528954
CGCAGAG201750.019.4269648
GTAAGGT17500.019.2512424
AGTACTT111350.018.64498312-13
Produced by FastQC (version 0.11.5)