##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939170_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3648721 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52962147558007 34.0 31.0 34.0 31.0 34.0 2 32.745096706489754 34.0 31.0 34.0 31.0 34.0 3 32.855319987469585 34.0 31.0 34.0 31.0 34.0 4 36.29194476639896 37.0 37.0 37.0 35.0 37.0 5 36.250354850370854 37.0 37.0 37.0 35.0 37.0 6 36.288030244022494 37.0 37.0 37.0 35.0 37.0 7 36.24311423098669 37.0 37.0 37.0 35.0 37.0 8 36.213413138466876 37.0 37.0 37.0 35.0 37.0 9 37.972348118696935 39.0 38.0 39.0 35.0 39.0 10-11 37.89139016110029 39.0 38.0 39.0 35.0 39.0 12-13 37.8095824536872 39.0 38.0 39.0 35.0 39.0 14-15 39.254108357421686 40.5 39.0 41.0 36.0 41.0 16-17 39.339422224938545 41.0 39.0 41.0 36.0 41.0 18-19 39.35638597744251 41.0 39.0 41.0 36.0 41.0 20-21 39.31224626382779 41.0 39.0 41.0 36.0 41.0 22-23 39.24668890825032 41.0 39.0 41.0 36.0 41.0 24-25 39.17511245721445 41.0 39.0 41.0 36.0 41.0 26-27 39.03617733446871 40.5 39.0 41.0 35.0 41.0 28-29 38.891327399381865 40.0 38.0 41.0 35.0 41.0 30-31 38.714205196834726 40.0 38.0 41.0 35.0 41.0 32-33 38.63943666835584 40.0 38.0 41.0 35.0 41.0 34-35 38.546423527586796 40.0 38.0 41.0 34.0 41.0 36-37 38.404535589320204 40.0 38.0 41.0 34.0 41.0 38-39 38.272811349511244 40.0 38.0 41.0 34.0 41.0 40-41 38.08985121087636 40.0 38.0 41.0 33.0 41.0 42-43 37.910898366852386 40.0 38.0 41.0 33.0 41.0 44-45 37.681279138635155 40.0 37.0 41.0 33.0 41.0 46-47 37.41581461010584 40.0 37.0 41.0 32.5 41.0 48-49 37.097890466275715 40.0 36.5 41.0 31.5 41.0 50-51 36.839518560065294 39.5 36.0 41.0 31.0 41.0 52-53 36.55908070252563 39.0 36.0 41.0 31.0 41.0 54-55 36.297830116361325 39.0 35.0 40.0 30.0 41.0 56-57 36.03419211827925 39.0 35.0 40.0 30.0 41.0 58-59 35.6831773380316 38.0 35.0 40.0 29.0 41.0 60-61 35.29112434192694 38.0 34.5 40.0 28.0 41.0 62-63 34.87865446549627 37.0 34.0 40.0 28.0 41.0 64-65 34.414026586302434 37.0 34.0 39.5 26.5 41.0 66-67 33.982796574470896 36.0 33.5 39.0 26.0 40.5 68-69 33.4840690751636 35.5 33.0 39.0 26.0 40.0 70-71 32.96223183411392 35.0 32.5 38.0 25.0 40.0 72-73 32.390065998469055 35.0 32.0 37.0 23.5 39.5 74-75 31.85403926471769 35.0 31.0 37.0 22.0 39.0 76-77 29.86139307444992 32.5 28.5 35.0 19.5 37.0 78-79 30.85855262707124 34.0 30.5 35.5 20.0 37.5 80-81 30.888475167051688 34.0 31.0 35.0 20.0 37.0 82-83 30.511769603650155 34.0 31.0 35.0 18.0 37.0 84-85 30.045049347428865 34.0 30.0 35.0 14.5 36.0 86-87 29.350665068663787 34.0 29.0 35.0 7.0 36.0 88-89 28.657286210702324 33.5 29.0 35.0 2.0 35.0 90-91 28.095224052483047 33.0 27.5 35.0 2.0 35.0 92-93 27.64293693598387 33.0 27.0 35.0 2.0 35.0 94-95 27.097406186989907 33.0 26.0 34.0 2.0 35.0 96-97 26.283972794850577 32.0 25.0 34.0 2.0 35.0 98-99 25.046850663561287 32.0 19.5 34.0 2.0 35.0 100-101 19.105993579668052 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1632901962851463 1101 2 0.15232641631875765 1101 3 0.18299502455154482 1101 4 0.08590068521850469 1101 5 0.13356787937381398 1101 6 0.142400978885604 1101 7 0.18511644236840397 1101 8 0.20772998207341686 1101 9 0.2505240555544077 1101 10-11 0.23129564617584464 1101 12-13 0.26488736158560755 1101 14-15 0.3293012358723004 1101 16-17 0.2767654808080877 1101 18-19 0.27926461785353496 1101 20-21 0.28769097244563824 1101 22-23 0.3633238223113935 1101 24-25 0.34611064770523825 1101 26-27 0.2660369494654802 1101 28-29 0.34260683569066686 1101 30-31 0.2915292419786937 1101 32-33 0.3265750173276203 1101 34-35 0.3427775117051013 1101 36-37 0.3756897141833875 1101 38-39 0.33697538751314227 1101 40-41 0.4245984049491014 1101 42-43 0.3841929250090601 1101 44-45 0.3500398091417054 1101 46-47 0.42054993546873476 1101 48-49 0.3254846617221574 1101 50-51 0.2782609177135029 1101 52-53 0.2884915650458524 1101 54-55 0.2003698748461673 1101 56-57 0.19125558655265706 1101 58-59 0.2457518311748288 1101 60-61 0.24463370810865825 1101 62-63 0.2347896557990765 1101 64-65 0.20626573693462547 1101 66-67 0.1730501671189515 1101 68-69 0.33721659567020623 1101 70-71 0.34463638617668124 1101 72-73 0.3161559674754244 1101 74-75 0.03316999868975046 1101 76-77 0.3370144860770594 1101 78-79 0.3415688974513742 1101 80-81 0.5628122413654957 1101 82-83 0.4721588187468271 1101 84-85 0.4098160494452969 1101 86-87 0.5365026828793447 1101 88-89 0.5140771369894672 1101 90-91 0.6738020182961186 1101 92-93 0.4142927284463518 1101 94-95 0.30073736868530077 1101 96-97 0.27933480898632723 1101 98-99 0.1514518799838065 1101 100-101 -0.5357751269407593 1102 1 0.18675976841836217 1102 2 0.11985286718449117 1102 3 0.13489375262271608 1102 4 0.1257909885675872 1102 5 0.1374066993821046 1102 6 0.16290121635600485 1102 7 0.1870146981116605 1102 8 0.1645353932228062 1102 9 0.21898883104690015 1102 10-11 0.27444757477111636 1102 12-13 0.20370526952668655 1102 14-15 0.226672168249614 1102 16-17 0.2608760180016958 1102 18-19 0.2008600430662355 1102 20-21 0.2504356056448316 1102 22-23 0.23601637482571647 1102 24-25 0.23262185685063486 1102 26-27 0.3067630722341619 1102 28-29 0.3306104101075462 1102 30-31 0.17023573479920628 1102 32-33 0.22151863644882752 1102 34-35 0.32705034881005446 1102 36-37 0.31895016765436424 1102 38-39 0.323272350726036 1102 40-41 0.2985983008359696 1102 42-43 0.4406842481442652 1102 44-45 0.34464687954891104 1102 46-47 0.37767019839660065 1102 48-49 0.3570313426940004 1102 50-51 0.3354962578107106 1102 52-53 0.3565909496729276 1102 54-55 0.30958595722980675 1102 56-57 0.38947658858326406 1102 58-59 0.15613564885865827 1102 60-61 0.1085227215000586 1102 62-63 0.07041687812703401 1102 64-65 0.30754153483214 1102 66-67 0.3422931480748943 1102 68-69 0.30802954436916963 1102 70-71 0.17412118495595053 1102 72-73 0.25592335502168595 1102 74-75 0.259224179324427 1102 76-77 0.21984314540743455 1102 78-79 0.2628557518419221 1102 80-81 0.42986670599989196 1102 82-83 0.3872312949530574 1102 84-85 0.3803518823513592 1102 86-87 0.517409831853783 1102 88-89 0.44646234070449964 1102 90-91 0.6097083763725344 1102 92-93 0.4318700600830603 1102 94-95 0.4816983027449062 1102 96-97 0.5711191975236716 1102 98-99 0.466330138971049 1102 100-101 -0.1986391184091083 1103 1 0.0719441254586215 1103 2 0.07144281847611467 1103 3 0.09366683829761513 1103 4 0.07245033780255028 1103 5 0.10344926749738192 1103 6 0.0785416260258458 1103 7 0.0901462575979437 1103 8 0.09981826286116302 1103 9 0.10482173387067917 1103 10-11 0.11805012401782022 1103 12-13 0.14300224077750556 1103 14-15 0.011381601129947683 1103 16-17 0.194091014128837 1103 18-19 0.23891570930549477 1103 20-21 0.22586741731272042 1103 22-23 0.2251027483835557 1103 24-25 0.23950341301394218 1103 26-27 0.2670182528960581 1103 28-29 0.1483814014728395 1103 30-31 0.23254732840921832 1103 32-33 0.22234824731446423 1103 34-35 0.29326064088801473 1103 36-37 0.21759655109350007 1103 38-39 0.34622591900288313 1103 40-41 0.32802526578840485 1103 42-43 0.17011773104582062 1103 44-45 0.19097948458512093 1103 46-47 0.3296923330367534 1103 48-49 0.13345525120089974 1103 50-51 0.11012472732635814 1103 52-53 0.18161409479210988 1103 54-55 0.11537051892578631 1103 56-57 0.08766699126748279 1103 58-59 0.0792667770969544 1103 60-61 0.1187948911668073 1103 62-63 0.07817028382930147 1103 64-65 0.22613638708982364 1103 66-67 0.27260755838411654 1103 68-69 0.16751531165927958 1103 70-71 0.08624652964665813 1103 72-73 0.16852036489775912 1103 74-75 0.04625865917682148 1103 76-77 0.024725040070876503 1103 78-79 0.06996310275934547 1103 80-81 0.09686271616828535 1103 82-83 -0.04427717015951771 1103 84-85 -0.15894411416084608 1103 86-87 -0.3639383379858572 1103 88-89 -0.15831255916572573 1103 90-91 0.07736925673478723 1103 92-93 0.216330540782792 1103 94-95 0.17263276102505998 1103 96-97 -0.048631822727358553 1103 98-99 -0.2925859871341352 1103 100-101 -1.019608827766671 1104 1 -0.011399455743088538 1104 2 0.021593228913843632 1104 3 0.04793024837546511 1104 4 0.025586906409131416 1104 5 0.013970534863283035 1104 6 0.017291883636048055 1104 7 0.0411450501870263 1104 8 0.0363411286118307 1104 9 0.07487421143773787 1104 10-11 0.15036582281925348 1104 12-13 0.13520424902047523 1104 14-15 0.10192879772307606 1104 16-17 0.1325556035737847 1104 18-19 0.19119188101850426 1104 20-21 0.1793627319873039 1104 22-23 0.16590455856702846 1104 24-25 0.23651895104399046 1104 26-27 0.18819588987047808 1104 28-29 0.1870096458419468 1104 30-31 0.1181378642978217 1104 32-33 0.1690994751504391 1104 34-35 0.21226770866614686 1104 36-37 0.15299497832133824 1104 38-39 0.16193899678123813 1104 40-41 0.24268158338016832 1104 42-43 0.16371712448509612 1104 44-45 0.2329196986623856 1104 46-47 0.17310815534974466 1104 48-49 0.163927605844421 1104 50-51 0.06431279790292876 1104 52-53 0.046781265088498 1104 54-55 0.10650689020788917 1104 56-57 0.22505924376590514 1104 58-59 0.14932374959111172 1104 60-61 0.19176451987734566 1104 62-63 0.1307285616898639 1104 64-65 0.1667934549668999 1104 66-67 0.2580879544239494 1104 68-69 0.17744372923856844 1104 70-71 0.18433884301035874 1104 72-73 -0.002506663749059612 1104 74-75 -0.0988086106581747 1104 76-77 -0.1145676619485343 1104 78-79 0.03357836413935189 1104 80-81 0.1477571642548483 1104 82-83 0.08620858445534196 1104 84-85 -0.14858556880971818 1104 86-87 -0.19224314832113265 1104 88-89 0.019876810699212655 1104 90-91 0.2879341164124938 1104 92-93 0.07161466682054751 1104 94-95 0.061151360270077504 1104 96-97 -0.24428497379846448 1104 98-99 -0.5087518369396768 1104 100-101 -0.8219519003914044 1105 1 0.1054340355248442 1105 2 0.11127203649952122 1105 3 0.09056099476342183 1105 4 0.09093542591383397 1105 5 0.02660368487742204 1105 6 0.09734845604353382 1105 7 0.12125672442142843 1105 8 0.08636891323613582 1105 9 0.1289672164900253 1105 10-11 0.17883490530103074 1105 12-13 0.17538098655725776 1105 14-15 0.2167994324763427 1105 16-17 0.2128948762188827 1105 18-19 0.21754417243651147 1105 20-21 0.2159205469274994 1105 22-23 0.24374986679477928 1105 24-25 0.20167661867444764 1105 26-27 0.2405820866201367 1105 28-29 0.22076400926842865 1105 30-31 0.31603800828168005 1105 32-33 0.3124526757036534 1105 34-35 0.22433321098537107 1105 36-37 0.3815765476321502 1105 38-39 0.35961127268803494 1105 40-41 0.32386297913371465 1105 42-43 0.3811562144941547 1105 44-45 0.3780294609622743 1105 46-47 0.40286964983353357 1105 48-49 0.4103726391203537 1105 50-51 0.2338308268072069 1105 52-53 0.29649353107877374 1105 54-55 0.2856192887661493 1105 56-57 0.24617528857295667 1105 58-59 0.11946772805806916 1105 60-61 0.01552640519759052 1105 62-63 0.11955956519570066 1105 64-65 0.13544954927390052 1105 66-67 0.11262012013788336 1105 68-69 0.07483365547403764 1105 70-71 0.011058024504627895 1105 72-73 -0.1203874043391906 1105 74-75 -0.1288348815552922 1105 76-77 -0.22637587559044903 1105 78-79 -0.15914611827651015 1105 80-81 0.11503148811587849 1105 82-83 0.02775188325519906 1105 84-85 -0.06441195508809017 1105 86-87 0.0326031823016919 1105 88-89 0.23189470791641398 1105 90-91 0.29180791866686917 1105 92-93 0.24090581642443354 1105 94-95 0.22715284990707474 1105 96-97 -0.08080374104924459 1105 98-99 0.11074132872589715 1105 100-101 -0.7473320265916676 1106 1 -0.002562508068862712 1106 2 0.012591810530452108 1106 3 0.02120472872434931 1106 4 0.02299993391871169 1106 5 -0.0017362413917041408 1106 6 0.02754624411719675 1106 7 0.05945609306279209 1106 8 0.0482482256023431 1106 9 0.04951042808201578 1106 10-11 0.0871250263186738 1106 12-13 0.12061731182419066 1106 14-15 0.05423635089039891 1106 16-17 0.011625558772280442 1106 18-19 -0.06893932496033273 1106 20-21 0.044823441884879855 1106 22-23 0.0545565208111185 1106 24-25 0.07548200910506608 1106 26-27 0.055003203699385494 1106 28-29 0.0382281099731685 1106 30-31 -0.07437020372282177 1106 32-33 0.008689621907898015 1106 34-35 -0.06311612617491136 1106 36-37 0.01347140514223355 1106 38-39 0.09625716907400772 1106 40-41 0.08509499691944455 1106 42-43 0.06372541860883985 1106 44-45 0.14262991168460104 1106 46-47 0.06372732492265953 1106 48-49 0.004591929862314714 1106 50-51 -0.0037209432983544843 1106 52-53 0.15196394536988578 1106 54-55 0.09732304521977397 1106 56-57 0.09561475152730736 1106 58-59 -0.0077020570455133 1106 60-61 -0.051190747001520265 1106 62-63 0.10314107081670443 1106 64-65 0.14566483648997064 1106 66-67 0.160050264690355 1106 68-69 -0.12837424562758315 1106 70-71 -0.14338258913775093 1106 72-73 -0.12608218108400138 1106 74-75 -0.2365637707722641 1106 76-77 -0.3233312419217498 1106 78-79 -0.14338903068961173 1106 80-81 0.028279120520991796 1106 82-83 0.017189739588545194 1106 84-85 -0.12737617086163056 1106 86-87 -0.25035486752092595 1106 88-89 -0.2561827746387202 1106 90-91 -0.06543455518211871 1106 92-93 0.18332486660746028 1106 94-95 -0.03673734405984774 1106 96-97 -0.42209038394950227 1106 98-99 -0.5748067678624231 1106 100-101 -1.2246118435237143 1107 1 -0.03946859549020587 1107 2 -0.026253897859255915 1107 3 -0.02375255925392139 1107 4 -0.02915220085361625 1107 5 0.005268405858586789 1107 6 0.03573211755369954 1107 7 0.025178737375142646 1107 8 0.07615170036982022 1107 9 0.04686520410029971 1107 10-11 0.04938570743193793 1107 12-13 0.04504672355233197 1107 14-15 0.04914223526647277 1107 16-17 0.04298001571943644 1107 18-19 0.14811983372783288 1107 20-21 0.10161851741565187 1107 22-23 0.07876403307152913 1107 24-25 0.054028453637450014 1107 26-27 0.02313873019782875 1107 28-29 0.043444632885176304 1107 30-31 0.05119406528315551 1107 32-33 0.06809229588971988 1107 34-35 0.011499403472804204 1107 36-37 0.0726074682603084 1107 38-39 0.12500315737612055 1107 40-41 0.1182160305441542 1107 42-43 0.11260002987039286 1107 44-45 0.06716436825880834 1107 46-47 0.11183181450208934 1107 48-49 0.18723972298819547 1107 50-51 0.14884515518689057 1107 52-53 0.15971559200482233 1107 54-55 -0.0022520514857191642 1107 56-57 0.04847742041228287 1107 58-59 0.09093144832379352 1107 60-61 0.0840240976792117 1107 62-63 0.039676041219749436 1107 64-65 -0.035373636443885914 1107 66-67 -0.10588653021942918 1107 68-69 -0.1202558329443022 1107 70-71 -0.18882084576625147 1107 72-73 -0.3391752441875724 1107 74-75 -0.25176809024948454 1107 76-77 -0.22559242028872717 1107 78-79 -0.11121460237598768 1107 80-81 -0.10635236268322856 1107 82-83 -0.21747891333043867 1107 84-85 -0.24322124318096883 1107 86-87 -0.33761539324240886 1107 88-89 -0.3690547255929282 1107 90-91 -0.13734891980583086 1107 92-93 0.01245659947447919 1107 94-95 -0.022301530190201646 1107 96-97 -0.16399847074513474 1107 98-99 -0.16222840378568648 1107 100-101 -0.7835138658959906 1108 1 -0.0976651740236747 1108 2 -0.03705114158213263 1108 3 -0.004052188679082747 1108 4 -0.03476914034584411 1108 5 -0.01556671940009835 1108 6 -0.01963486889265198 1108 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-0.4825185194320696 2316 80-81 -0.474964965218426 2316 82-83 -0.48990598424288123 2316 84-85 -0.5444382532369048 2316 86-87 -0.39243338482776124 2316 88-89 -0.6230777299634802 2316 90-91 -0.8340583418520744 2316 92-93 -0.7743808786742505 2316 94-95 -0.6879856272653555 2316 96-97 -0.716361768650355 2316 98-99 -0.4509119205606815 2316 100-101 0.6986398038274046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 23.0 5 50.0 6 112.0 7 289.0 8 775.0 9 1477.0 10 2596.0 11 3995.0 12 6042.0 13 8490.0 14 10702.0 15 14283.0 16 18071.0 17 25142.0 18 25502.0 19 19949.0 20 19683.0 21 20588.0 22 22059.0 23 24366.0 24 27865.0 25 32384.0 26 38281.0 27 45637.0 28 55519.0 29 67599.0 30 83020.0 31 102918.0 32 129360.0 33 170745.0 34 240323.0 35 364831.0 36 599439.0 37 895153.0 38 546967.0 39 24475.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10940009842574 18.622061677927643 14.102129364889757 24.166408858756864 2 15.929088576517634 20.038336721278498 39.20321668880685 24.829358013397023 3 19.896341759208227 23.075839451687315 29.230078156153894 27.797740632950557 4 13.57275604245981 15.954028822702531 35.7363854347866 34.73682970005106 5 14.30410820668393 36.72878797803394 34.01506993820574 14.95203387707638 6 33.85827526960817 34.99922301540732 16.579727526440085 14.562774188544426 7 29.614541643496445 31.831702122469764 20.088655723471323 18.46510051056247 8 26.652955926199894 35.062066954420466 19.358619088716292 18.926358030663348 9 26.165798919676238 16.862264886791838 19.96285821798926 37.00907797554267 10-11 25.536139924099434 26.06032360380528 26.749496604426593 21.654039867668697 12-13 26.1967275656319 23.160512957828235 27.364342190044127 23.278417286495735 14-15 22.701561161336084 24.8282125056253 25.40330869072605 27.066917642312564 16-17 22.719716342885366 27.909704750864744 26.42664144807209 22.943937458177803 18-19 22.736076559430003 26.735710951865048 28.19244058397449 22.335771904730453 20-21 23.111989105223447 25.836190270508485 28.50046632778993 22.551354296478134 22-23 22.784847073810248 25.863638244743843 28.339752477649014 23.01176220379689 24-25 22.8646864017126 26.005486032706532 28.445313912096037 22.68451365348483 26-27 22.442845294887963 26.2644829285486 28.347415423863247 22.945256352700184 28-29 22.44141473702905 26.8418203038572 28.14468798034818 22.57207697876557 30-31 22.649525683109232 26.296803729306788 28.006210943505955 23.04745964407802 32-33 22.3401600643294 26.25503926650452 28.223592352442452 23.181208316723627 34-35 23.00514008989161 26.353373128314487 28.010084197153045 22.631402584640856 36-37 23.130792121686472 25.963577398029187 28.044005389645903 22.861625090638437 38-39 22.621751994054772 26.34590644821817 28.114273997394168 22.918067560332887 40-41 22.99431902511933 26.253222126761948 28.08425488888228 22.66820395923644 42-43 22.740858366993024 26.27966553598977 27.815939816980272 23.16353628003693 44-45 22.783865766863936 26.42527121871469 27.957662952205347 22.833200062216026 46-47 23.223139544508463 26.68720820535984 27.35657147430398 22.733080775827712 48-49 22.962618235078804 26.75230748801496 27.314932629832644 22.970141647073593 50-51 22.95577765000008 26.82377385028851 27.483173449816896 22.73727504989451 52-53 23.295108066634857 26.646515313174124 27.265403411222728 22.79297320896829 54-55 22.86634138373419 26.754402433071757 27.352982044941225 23.026274138252827 56-57 22.83926847731176 26.986355207500278 27.223479589269438 22.95089672591853 58-59 22.94205003544439 26.586867802778187 27.26904723045238 23.20203493132504 60-61 22.623865778191206 26.787189881619135 27.63213322637131 22.956811113818354 62-63 22.662430971479218 26.752890708484976 27.70618249429313 22.87849582574267 64-65 22.490906170757885 26.944799524643237 27.439341582644218 23.124952721954664 66-67 22.390884923724247 26.93647339218363 27.31845170106696 23.35418998302517 68-69 22.82997099278088 26.501761167272313 27.510552408892842 23.157715431053962 70-71 22.746298730689947 26.62709017107258 27.183724569296942 23.442886528940534 72-73 22.64065956262482 26.64652901660609 27.330042499823907 23.382768920945175 74-75 22.521741179991565 27.07541903039449 27.047217367400794 23.355622422213155 76-77 22.633397540327575 26.684816355788772 27.20382174953292 23.477964354350732 78-79 22.623458245297023 26.82450338750193 27.22336680083638 23.32867156636467 80-81 22.76723516431353 26.71157385602393 27.080402788875684 23.440788190786854 82-83 22.67686402988883 26.75553981792524 27.245286773091177 23.322309379094758 84-85 22.46888704288434 26.898028103546423 27.115570086065777 23.51751476750346 86-87 22.653537354298418 26.80822641108762 26.997261731472992 23.540974503140962 88-89 22.501646181993074 26.835071485926086 26.816741584862143 23.846540747218693 90-91 22.347016994824074 26.728830097174228 26.985809893027152 23.938343014974546 92-93 22.49674612007879 26.55566155921486 27.104114016939086 23.843478303767267 94-95 22.290060310182707 26.261192108302073 27.39535681069569 24.053390770819526 96-97 22.31768949110921 25.98856585323072 27.420054155977986 24.27369049968208 98-99 22.12087601275408 26.28316442450683 27.06391895884413 24.532040603894963 100-101 22.480998434379252 25.122656279441035 27.821553493184243 24.574791792995473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 244.0 1 183.0 2 188.5 3 276.5 4 247.5 5 223.0 6 408.0 7 849.0 8 1412.5 9 1709.5 10 1574.0 11 1545.0 12 1753.5 13 1960.0 14 2153.5 15 2403.5 16 2689.0 17 2945.5 18 3278.5 19 3741.5 20 4413.5 21 5385.5 22 6659.0 23 8351.5 24 10664.0 25 13700.5 26 18045.5 27 23635.5 28 28328.0 29 32763.5 30 38213.0 31 45807.5 32 57332.0 33 62634.5 34 63263.5 35 68102.0 36 75437.0 37 83059.5 38 88803.5 39 93392.0 40 98395.5 41 105140.0 42 109901.5 43 114280.0 44 119898.0 45 126560.5 46 131901.5 47 138794.0 48 147486.0 49 146276.5 50 139639.0 51 135446.0 52 134473.5 53 136122.0 54 136576.5 55 128702.0 56 117701.0 57 107644.0 58 98731.5 59 88254.5 60 72555.5 61 60584.0 62 50641.5 63 37142.0 64 25995.0 65 19535.5 66 14617.5 67 11907.0 68 9539.0 69 7405.0 70 5716.0 71 3809.5 72 2715.5 73 2110.0 74 1552.0 75 1147.0 76 847.0 77 528.0 78 305.0 79 195.0 80 123.5 81 73.0 82 39.0 83 20.0 84 12.5 85 9.5 86 7.0 87 3.0 88 3.0 89 3.0 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4254915626599019 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.399853263650468E-4 16-17 0.002480321186519879 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.3703431969723088E-5 26-27 0.0031517893530363106 28-29 1.3703431969723088E-5 30-31 0.0 32-33 1.3703431969723088E-5 34-35 0.01201790983744715 36-37 0.02702316784429393 38-39 0.020609961682463526 40-41 0.00864686557289527 42-43 0.008674272436834716 44-45 0.003932884975310526 46-47 0.004905828645160866 48-49 0.0030284584653088027 50-51 6.303578706072621E-4 52-53 0.0 54-55 0.0 56-57 8.222059181833854E-5 58-59 0.0013018260371236935 60-61 0.0020281079315190172 62-63 0.0013155294690934166 64-65 0.0025488383463684945 66-67 0.002315880002883202 68-69 0.004015105567128865 70-71 1.7814461560640015E-4 72-73 0.0 74-75 0.0 76-77 0.09536218307730297 78-79 0.01658115268336494 80-81 2.8777207136418485E-4 82-83 0.0 84-85 0.0 86-87 0.10687306593187038 88-89 0.4196950109367091 90-91 0.42634117544202477 92-93 0.0 94-95 1.3703431969723088E-5 96-97 2.7406863939446175E-5 98-99 1.9184804757612327E-4 100-101 2.3295834348529252E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3648721.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.897306642925514 #Duplication Level Percentage of deduplicated Percentage of total 1 71.1809812591655 34.093772865145844 2 14.897523840882085 14.27102535254045 3 5.632835475628742 8.09392944136017 4 2.7266038744317664 5.223879274697884 5 1.4811500625071237 3.547154936404599 6 0.9473839244393872 2.7226283002470897 7 0.5983696456049358 2.0062206080950795 8 0.4154692713744702 1.5919887273388666 9 0.3087475262988846 1.3309357448144143 >10 1.6301429222023354 14.134616905572159 >50 0.11524436081123113 3.788192263941899 >100 0.05942895882522093 5.238859208967968 >500 0.0032024033571658585 1.077731863352599 >1k 0.0028021029231641036 2.5025280532161873 >5k 1.1437154788424914E-4 0.37653645430485333 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7476 0.20489371481129964 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6271 0.17186844376426696 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4511 0.12363236323084172 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4089 0.11206666664839542 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3996 0.10951782830202694 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3884 0.10644825954080897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3735 0.10236463681383148 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0962745575778471E-4 2 0.0 0.0 0.0 0.0 1.0962745575778471E-4 3 0.0 0.0 0.0 0.0 1.0962745575778471E-4 4 0.0 0.0 0.0 0.0 1.0962745575778471E-4 5 0.0 0.0 0.0 0.0 1.0962745575778471E-4 6 0.0 0.0 0.0 0.0 1.370343196972309E-4 7 0.0 0.0 0.0 0.0 1.370343196972309E-4 8 0.0 0.0 0.0 0.0 1.370343196972309E-4 9 0.0 0.0 0.0 0.0 1.370343196972309E-4 10-11 0.0 0.0 0.0 0.0 1.370343196972309E-4 12-13 0.0 0.0 0.0 0.0 3.8369609515224654E-4 14-15 0.0 0.0 0.0 0.0 4.3850982303113886E-4 16-17 0.0 0.0 0.0 1.370343196972309E-5 5.207304148494774E-4 18-19 0.0 0.0 0.0 2.740686393944618E-5 5.755441427283698E-4 20-21 0.0 0.0 0.0 9.592402378806162E-5 6.440613025769852E-4 22-23 0.0 0.0 0.0 3.0147550333390797E-4 6.577647345467083E-4 24-25 0.0 0.0 0.0 3.8369609515224654E-4 6.577647345467083E-4 26-27 0.0 0.0 0.0 6.851715984861545E-4 6.851715984861545E-4 28-29 0.0 0.0 0.0 0.002658465802126279 6.988750304558776E-4 30-31 0.0 0.0 0.0 0.007783549358802716 7.262818943953237E-4 32-33 0.0 0.0 0.0 0.019760348900340695 7.399853263650468E-4 34-35 0.0 0.0 0.0 0.03876700904234662 7.947990542439392E-4 36-37 0.0 0.0 0.0 0.07113451535483256 7.947990542439392E-4 38-39 0.0 0.0 0.0 0.12087797340492737 8.359093501531085E-4 40-41 0.0 0.0 0.0 0.17440357867866574 8.496127821228316E-4 42-43 0.0 0.0 0.0 0.23058764975453044 8.496127821228316E-4 44-45 0.0 0.0 0.0 0.30159883422163547 8.907230780320008E-4 46-47 0.0 0.0 0.0 0.3871767668725562 0.0010003505337897855 48-49 0.0 0.0 0.0 0.48051084201834016 0.0010688676936384009 50-51 0.0 0.0 0.0 0.5838210156380825 0.0011784951493961856 52-53 0.0 0.0 0.0 0.6843905028638801 0.0012059020133356319 54-55 0.0 0.0 0.0 0.7993348902259175 0.0013155294690934166 56-57 0.0 0.0 0.0 0.9392058203408811 0.0013292329010631395 58-59 0.0 0.0 0.0 1.0927390721296586 0.0013429363330328627 60-61 0.0 0.0 0.0 1.2636619790880146 0.001370343196972309 62-63 0.0 0.0 0.0 1.4353933885325845 0.0013977500609117551 64-65 0.0 0.0 0.0 1.6214585878174845 0.0014388603568209245 66-67 0.0 0.0 0.0 1.8411246022921457 0.0014662672207603705 68-69 0.0 0.0 0.0 2.0879782257947372 0.0014799706527300937 70-71 0.0 0.0 0.0 2.3503304308550863 0.0014936740846998168 72-73 0.0 0.0 0.0 2.6276741904903114 0.0015758946765181553 74-75 0.0 0.0 0.0 2.9165699432760137 0.0016581152683364938 76-77 0.0 0.0 0.0 3.2330644080487385 0.0016992255642456632 78-79 0.0 0.0 0.0 3.6006863775004994 0.0016992255642456632 80-81 0.0 0.0 0.0 3.9957426177556465 0.001712928996215386 82-83 0.0 0.0 0.0 4.4023371477293 0.0018088530200034477 84-85 0.0 0.0 0.0 4.8210592149961595 0.0018088530200034477 86-87 0.0 0.0 0.0 5.265461513774278 0.0018499633159126169 88-89 0.0 2.740686393944618E-5 0.0 5.747274181829742 0.0019047770437915093 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15000 0.0 26.591658 1 GGTATCA 10745 0.0 24.703585 1 ATCAACG 17095 0.0 23.065899 3 TACATGG 11120 0.0 22.95704 2 GTACATG 11395 0.0 22.876217 1 TCAACGC 17220 0.0 22.870907 4 CAACGCA 17440 0.0 22.609608 5 TATCAAC 17685 0.0 22.430538 2 AACGCAG 18060 0.0 21.885963 6 ACATGGG 11235 0.0 21.6662 3 GAGTACT 10380 0.0 20.709757 12-13 AGAGTAC 15220 0.0 19.876352 10-11 GTATAGG 1010 0.0 19.817055 1 ACGCAGA 19985 0.0 19.659143 7 GTACTTT 10930 0.0 19.559095 14-15 TAAGGTG 1820 0.0 19.55367 5 CATGGGG 7435 0.0 19.52895 4 CGCAGAG 20175 0.0 19.426964 8 GTAAGGT 1750 0.0 19.251242 4 AGTACTT 11135 0.0 18.644983 12-13 >>END_MODULE